Publications by authors named "Tao Xu"

2,030 Publications

  • Page 1 of 1

Health benefits and phenolic compounds of Moringa oleifera leaves: A comprehensive review.

Phytomedicine 2021 Oct 1;93:153771. Epub 2021 Oct 1.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China. Electronic address:

Background: Moringa oleifera Lam (MO) is native to India and is a cash crop widely cultivated in tropical and sub-tropical areas. The health improving properties of MO has been studied from a long time ago for the numerous phenolic compounds, including vitamins, flavonoids, phenolic acids, isothiocyanates, tannins and saponins, which are present in considerable amounts in the plant. A growing spectrum of therapeutic characteristics of MO leaves has been found and used in the remission or treatment of oxidative stress, liver disease, neurological disease, hyperglycemia and cancer.

Hypothesis: This review focused on researches applying MO or MO leaf extract as a functional food or cure against various disease and cellular injuries. We believed it would help the discovery of therapeutic application of MO and understanding of MO phytochemistry.

Methods: The data collected in this review were extracted from researches indexed in Web of Science, google scholar, PubMed, Science Direct and Scopus to find out health benefits and biological activities of MO leaves polyphenols. The studies reporting mechanistic route of phenolic compounds of MO leaves were also considered in the present study.

Results: It has been reported that polyphenols of MO leaf have protective characteristics against neurodegenerative disorders through reducing DNA damage, activation of AchE activity and inhibition of caspase-3 activity. It has been reported that, they protected the kidney from damage caused by melamine through suppressed the pro-inflammatory cytokine, metallopeptidase inhibitor 1 (TIMP-1), and kidney injury molecule 1 (KIM-1). Similarly, methanol extract of MO leaves has low hypoglycemic attributes and attenuate the risk of diabetes caused by alloxan by enhancing lipid metabolism and stimulating insulin release, glucose uptake, and glycogen synthesis. In addition, MO leaves are becoming the best phytomedicine to reduce hypertension, which are naturally known as angiotensin-1converting enzyme (ACE), acetylcholinesterase, arginase and phosphodiesterase 5 (PDE5) inhibitors.

Conclusion: MO leaves extract as a health promoting food additives for human and animals due to its great protective effect against many diseases and the widely persistent environmental toxins which disrupted cellular metabolic function. More studies are required to use the phenolic compounds of MO leaves to develop and produce drugs for controlling and treatment of various diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2021.153771DOI Listing
October 2021

Prevalence of cognitive impairment in Chinese older inpatients and its relationship with 1-year adverse health outcomes: a multi-center cohort study.

BMC Geriatr 2021 Oct 25;21(1):595. Epub 2021 Oct 25.

Department of Nursing, Chinese Academy of Medical Sciences - Peking Union Medical College, Peking Union Medical College Hospital, Beijing, 100730, China.

Background: Previous studies on the relationship between cognitive impairment and adverse outcomes among geriatric inpatients are not representative of older inpatients in China because of insufficient sample sizes or single-center study designs. The purpose of our study was to examine the prevalence of cognitive impairment and the relationship between cognitive impairment and 1-year adverse health outcomes in older inpatients.

Methods: This study was a large-scale multi-center cohort study conducted from October 2018 to February 2020. Six tertiary hospitals across China were selected using a two-stage cluster sampling method, and eligible older inpatients were selected for the baseline survey and follow-up. The Mini Cognitive Scale and the FRAIL scale were used to screen for cognitive impairment and frailty, respectively. The EuroQol-5 Dimension-5 Level questionnaire was used to assess health-related quality of life (HRQoL). We used a generalized estimating model to evaluate the relationship between cognitive impairment and adverse outcomes.

Results: The study included 5008 men (58.02%) and 3623 women (41.98%), and 70.64% were aged 65-75 years, and 26.27% were aged 75-85 years. Cognitive impairment was observed in 1756 patients (20.35%). There were significant differences between participants with cognitive impairment and those with normal cognitive function for age, gender, surgery status, frailty, depression, handgrip strength and so on. After adjusting for multiple covariates, compared with patients with normal cognitive function, the odds ratio for 1-year mortality was 1.216 (95% confidence interval [CI]: 1.076-1.375) and for 1-year incidence of frailty was 1.195 (95% CI: 1.037-1.376) in patients with cognitive impairment. Similarly, the regression coefficient of 1-year HRQoL was - 0.013 (95% CI: - 0.024-- 0.002). In the stratified analysis, risk of adverse outcome within 1 year was higher in older patients with cognitive impairment aged over 75 years than those aged 65-74 years.

Conclusions: We revealed that cognitive impairment was highly correlated with occurrence of 1-year adverse health outcomes (death, frailty, and decreased HRQoL) in older inpatients, which provides a basis for formulating effective intervention measures.

Trial Registration: Chinese Clinical Trial Registry, ChiCTR1800017682 , registered 09 August 2018.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12877-021-02556-5DOI Listing
October 2021

Study of Auditory Brain Cognition Laws-Based Recognition Method of Automobile Sound Quality.

Front Hum Neurosci 2021 8;15:663049. Epub 2021 Oct 8.

Hubei Key Laboratory of Advanced Technology for Automotive Components, Wuhan University of Technology, Wuhan, China.

The research shows that subjective feelings of people, such as emotions and fatigue, can be objectively reflected by electroencephalography (EEG) physiological signals Thus, an evaluation method based on EEG, which is used to explore auditory brain cognition laws, is introduced in this study. The brain cognition laws are summarized by analyzing the EEG power topographic map under the stimulation of three kinds of automobile sound, namely, quality of comfort, powerfulness, and acceleration. Then, the EEG features of the subjects are classified through a machine learning algorithm, by which the recognition of diversified automobile sound is realized. In addition, the Kalman smoothing and minimal redundancy maximal relevance (mRMR) algorithm is used to improve the recognition accuracy. The results show that there are differences in the neural characteristics of diversified automobile sound quality, with a positive correlation between EEG energy and sound intensity. Furthermore, by using the Kalman smoothing and mRMR algorithm, recognition accuracy is improved, and the amount of calculation is reduced. The novel idea and method to explore the cognitive laws of automobile sound quality from the field of brain-computer interface technology are provided in this study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnhum.2021.663049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533456PMC
October 2021

The air quality changes and related mortality benefits during the coronavirus disease 2019 pandemic in China: Results from a nationwide forecasting study.

J Clean Prod 2021 Jul 1;308:127327. Epub 2021 May 1.

Department of Occupational & Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.

Air quality changes during the coronavirus disease 2019 (COVID-19) pandemic in China has attracted increasing attention. However, more details in the changes, future air quality trends, and related death benefits on a national scale are still unclear. In this study, a total of 352 Chinese cities were included. We collected air pollutants (including fine particulate matter [PM], inhalable particulate matter [PM], nitrogen dioxide [NO], and ozone [O]) data for each city from January 2015 to July 2020. Convolutional neural network-quantile regression (CNN-QR) forecasting model was used to predict pollutants concentrations from February 2020 to January 2021 and the changes in air pollutants were compared. The relationships between the socioeconomic factors and the changes and the avoided mortality due to the changes were further estimated. We found sharp declines in all air pollutants from February 2020 to January 2021. Specifically, PM, PM, NO, and O would drop by 3.86 μg/m (10.81%), 4.84 μg/m (7.65%), 0.55 μg/m (2.18%), and 3.14 μg/m (3.36%), respectively. The air quality changes were significantly related to many of the socioeconomic factors, including the size of built-up area, gross regional product, population density, gross regional product per capita, and secondary industry share. And the improved air quality would avoid a total of 7237 p.m.-related deaths (95% confidence intervals [CI]: 4935, 9209), 9484 p.m.-related deaths (95%CI: 5362, 13604), 4249 NO-related deaths (95%CI: 3305, 5193), and 6424 O-related deaths (95%CI: 3480, 9367), respectively. Our study shows that the interventions to control COVID-19 would improve air quality, which had significant relationships with some socioeconomic factors. Additionally, improved air quality would reduce the number of non-accidental deaths.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jclepro.2021.127327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525877PMC
July 2021

Hedgehog artificial macrophage with atomic-catalytic centers to combat Drug-resistant bacteria.

Nat Commun 2021 Oct 22;12(1):6143. Epub 2021 Oct 22.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, 610065, Chengdu, China.

Pathogenic drug-resistant bacteria represent a threat to human health, for instance, the methicillin-resistant Staphylococcus aureus (MRSA). There is an ever-growing need to develop non-antibiotic strategies to fight bacteria without triggering drug resistance. Here, we design a hedgehog artificial macrophage with atomic-catalytic centers to combat MRSA by mimicking the "capture and killing" process of macrophages. The experimental studies and theoretical calculations reveal that the synthesized materials can efficiently capture and kill MRSA by the hedgehog topography and substantial generation of •O and HClO with its FeNO catalytic centers. The synthesized artificial macrophage exhibits a low minimal inhibition concentration (8 μg/mL Fe-Art M with HO (100 μM)) to combat MRSA and rapidly promote the healing of bacteria-infected wounds on rabbit skin. We suggest that the application of this hedgehog artificial macrophage with "capture and killing" capability and high ROS-catalytic activity will open up a promising pathway to develop antibacterial materials for bionic and non-antibiotic disinfection strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-26456-9DOI Listing
October 2021

RNF8-mediated regulation of Akt promotes lung cancer cell survival and resistance to DNA damage.

Cell Rep 2021 Oct;37(3):109854

Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. Electronic address:

Despite the tremendous success of targeted and conventional therapies for lung cancer, therapeutic resistance is a common and major clinical challenge. RNF8 is a ubiquitin E3 ligase that plays essential roles in the DNA damage response; however, its role in the pathogenesis of lung cancer is unclear. Here, we report that RNF8 is overexpressed in lung cancer and positively correlates with the expression of p-Akt and poor survival of patients with non-small-cell lung cancer. In addition, we identify RNF8 as the E3 ligase for regulating the activation of Akt by K63-linked ubiquitination under physiological and genotoxic conditions, which leads to lung cancer cell proliferation and resistance to chemotherapy. Together, our study suggests that RNF8 could be a very promising target in precision medicine for lung cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.celrep.2021.109854DOI Listing
October 2021

G-alpha interacting protein interacting protein, C terminus 1 regulates epileptogenesis by increasing the expression of metabotropic glutamate receptor 7.

CNS Neurosci Ther 2021 Oct 22. Epub 2021 Oct 22.

Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Chonqing, China.

Aims: It has been reported that the G-alpha interacting protein (GAIP) interacting protein, C terminus 1 (GIPC1/GIPC) engages in vesicular trafficking, receptor transport and expression, and endocytosis. However, its role in epilepsy is unclear. Therefore, in this study, we aimed to explore the role of GIPC1 in epilepsy and its possible underlying mechanism.

Methods: The expression patterns of GIPC1 in patients with temporal lobe epilepsy (TLE) and in mice with kainic acid (KA)-induced epilepsy were detected. Behavioral video monitoring and hippocampal local field potential (LFP) recordings were carried out to determine the role of GIPC1 in epileptogenesis after overexpression of GIPC1. Coimmunoprecipitation (Co-IP) assay and high-resolution immunofluorescence staining were conducted to investigate the relationship between GIPC1 and metabotropic glutamate receptor 7 (mGluR7). In addition, the expression of mGluR7 after overexpression of GIPC1 was measured, and behavioral video monitoring and LFP recordings after antagonism of mGluR7 were performed to explore the possible mechanism mediated by GIPC1.

Results: GIPC1 was downregulated in the brain tissues of patients with TLE and mice with KA-induced epilepsy. After overexpression of GIPC1, prolonged latency period, decreased epileptic seizures and reduced seizure severity in behavioral analyses, and fewer and shorter abnormal brain discharges in LFP recordings of KA-induced epileptic mice were observed. The result of the Co-IP assay showed the interaction between GIPC1 and mGluR7, and the high-resolution immunofluorescence staining also showed the colocalization of these two proteins. Additionally, along with GIPC1 overexpression, the total and cell membrane expression levels of mGluR7 were also increased. And after antagonism of mGluR7, increased epileptic seizures and aggravated seizure severity in behavioral analyses and more and longer abnormal brain discharges in LFP recordings were observed.

Conclusion: GIPC1 regulates epileptogenesis by interacting with mGluR7 and increasing its expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/cns.13746DOI Listing
October 2021

Regulatory Effects of Acupuncture on Emotional Disorders in Patients With Menstrual Migraine Without Aura: A Resting-State fMRI Study.

Front Neurosci 2021 4;15:726505. Epub 2021 Oct 4.

College of Acupuncture, Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Menstrual migraine without aura (MMoA) refers to a specific type of migraine that is associated with the female ovarian cycle. It is particularly serious and has brought huge life pressure and mental burden to female patients. Acupuncture has been commonly used to prevent migraines and relieve concomitant emotional disorders; however, the physiological mechanism underlying this intervention remains unclear. This study aimed to use resting-state functional magnetic resonance imaging (rsfMRI) to investigate whether acupuncture can modulate brain function and if the potential influence on brain activity correlates with improving emotional symptoms in MMoA patients. Overall, 44 patients were randomly divided into a true acupuncture (TA) group and the sham acupuncture (SA) group. Patients underwent rsfMRI before and after 3-month treatment, the amplitude of low-frequency fuctuations (ALFF) and regional homogeneity (ReHo) in rsfMRI were calculated. Zung self-rating anxiety scale (SAS), Zung self-rating depression scale (SDS), frequency of migraine attacks, visual analog scale, and intensity of the migraine were used for evaluate the clinical effect. The clinical changes of variables were also used to further assess the correlation with brain activity in MMoA patients. After acupuncture treatment, the emotional symptoms of both groups of patients improved, and the clinical symptoms of migraine were alleviated. The major finding of our study was that patients with MMoA showed lower ALFF value in the left anterior cingulate and the value was positively correlated with the decreases in the SAS and SDS scores. In the SA group, common brain regions responded both in ALFF and regional homogeneity values mainly in the insula, and no significant correlations were observed between brain regions and clinical variables. These results indicated that both two acupuncture treatments were helpful in treating migraine and could improve emotion symptoms. TA had a relatively better effect in reducing the frequency of migraine attack than SA. The two therapies have different modulation effects as TA regulates emotional disorders by modulating the frontal-limbic regions, and SA may modulate pain perception through the placebo effect on insula and by indirectly regulating emotional disorders. These findings provided evidence that acupuncture is a complementary and alternative therapy to relieve clinical symptoms in female patients with migraines and could help enhance clinical diagnosis and treatment. [http://www.chictr.org.cn/index.aspx], identifier [ChiCTR-IOR-15006648. Registered 23 June 2015].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnins.2021.726505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521095PMC
October 2021

Effects of RPA1 gene polymorphisms on the sensitivity to oxaliplatin in the treatment of gastric cancer.

Minerva Gastroenterol (Torino) 2021 Oct 19. Epub 2021 Oct 19.

Department of Gestrointestin, Affiliated hospital of Jianghan University, Wuhan, China - [email protected]

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S2724-5985.21.02965-XDOI Listing
October 2021

Adipogenic differentiation was inhibited by downregulation of PPARγ signaling pathway in aging tendon stem/progenitor cells.

J Orthop Surg Res 2021 Oct 18;16(1):614. Epub 2021 Oct 18.

Department of Sports Medicine Center, Southwest Hospital, Army Medical University, Chongqing, 400038, China.

Background: Tendon stem/progenitor cells (TSPCs) play a vital role in tendon repair and regeneration. Previously we found more adipocytes accumulated in the patellar tendon injury sites in aging rats compared with the young ones, of which the mechanism is still unknown. Here, we want to identify whether erroneous differentiation of TSPCs by aging accounts for the adipocyte accumulation.

Methods: TSPCs from young and aging rats were isolated and propagated. Both young and aging TSPCs were induced to differentiate into adipocytes, and Oil red O staining, quantitative real-time polymerase chain reaction (qRT-PCR), western-blot and immunofluorescent staining were used to evaluate the capability of TSPCs. RNA sequencing was utilized to screen out different genes and signaling pathways related to adipogenesis between young and aging TSPCs.

Results: The Oil red O staining showed there were more adipocytes formed in young TSPCs. Besides, adipogenic markers perilipin, peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding proteins alpha (C/EBPα) and Fatty acid-binding protein 4 (FABP4) were elevated both at gene and protein level. PPARγ signaling pathway was selected as our target via RNA sequencing. After adding the signaling activators, Rosiglitazone maleate (RM), inhibited adipogenesis of aging TSCs was reversed.

Conclusions: In conclusion, aging inhibited adipogenesis of TSPCs by down-regulating PPARγ signaling. It is not likely that the adipocyte accumulation in aging tendon during repair was due to the aging of TSPCs. This may provide new targets for curing aging tendon injuries or tendinopathies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-021-02720-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522149PMC
October 2021

Career choice regret during COVID-19 among healthcare students and professionals in mainland China: a cross-sectional study.

BMC Med Educ 2021 Oct 18;21(1):534. Epub 2021 Oct 18.

Urology Department, Peking University People's Hospital, No. 11 Xizhimen South Street Xicheng District, Beijing, 100044, China.

Background: The COVID-19 epidemic affected the career choice of healthcare professionals and students. Career choice regret of healthcare professionals and students during COVID-19 outbreak and its affected factors are largely unexplored.

Methods: Convenience sample of nurses, doctors, and medical students were recruited from hospitals and universities nationwide. The data collected including demographic information, professional value before and after the COVID-19 outbreak, the Connor-Davidson Resilience Scale, and career choice regret level by an online questionnaire. Multinominal logistic regression was employed to explore the factors associated with career choice regret.

Results: In total, 9322 participants of convenience sampling were enrolled in, including 5786 nurses, 1664 doctors, and 1872 medical students. 6.7% participants had career choice regret. Multinominal logistic regression analysis showed, compared to participants with no regret, that as levels of psychological resilience increased, the odds of experiencing career choice regret decreased (OR = 0.95, 95% CI 0.94-0.96), while participants with lower professional value evaluation after the COVID-19 outbreak had higher probability to experience career choice regret (OR = 1.55,95% CI 1.50-1.61). Medical students were more likely to regret than nurses (OR = 1.65,95% CI 1.20-2.28), participants whose career/major choice was not their personal ideal had higher risk of experience career choice regret (OR = 1.59,95% CI 1.29-1.96), while participants who were very afraid of the coronavirus had higher risk to experience career choice regret then participants with no fear at all (OR = 2.00,95% CI 1.24-3.21). As for the medical students, results indicated that medical students major in nursing and undergraduates had higher risk to experience career choice regret compared to medical students major in clinical medicine and postgraduate (Master or PhD), with an odds ratios of 2.65(95% CI 1.56-4.49) and 6.85 (95% CI 2.48-18.91)respectively.

Conclusions: A minority of healthcare professionals and medical students regretted their career choices during the COVID-19 outbreak. Enhance personal psychological resilience and professional value would helpful to reduce career choice regret among healthcare professionals and students during pandemic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12909-021-02972-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521507PMC
October 2021

Adsorption Motifs and Molecular Orientation at the Ionic Liquid/Noble Metal Interface: [CCIm][NTf] on Pt(111).

Langmuir 2021 Oct 18. Epub 2021 Oct 18.

Interface Research and Catalysis, Erlangen Center for Interface Research and Catalysis, Friedrich-Alexander-Universität Erlangen-Nürnberg, Egerlandstraße 3, 91058 Erlangen, Germany.

In solid catalysts with ionic liquid layers (SCILLs), ionic liquid (IL) thin films are used to modify the activity and selectivity of catalytic materials. In this work, we investigated the adsorption behavior of the IL 1-ethyl-3-methyl-imidazolium bis(trifluoromethylsulfonyl)imide [CCIm][NTf] on Pt(111) by combining experimental and theoretical studies. Under ultrahigh vacuum (UHV) conditions, the IL was deposited onto a Pt(111) single crystal surface by physical vapor deposition (PVD) at different surface temperatures (200, 300, and 400 K). The adsorption process was monitored by infrared reflection absorption spectroscopy (IRAS). Complementary to the IRAS studies, we performed density functional theory (DFT) calculations and analyzed the adsorption motifs and orientation of the IL ions. In total, we calculated four different systems: (a) [CCIm] and [NTf] ions in the gas phase; [NTf] anions in (b) small (4 × 4) and (c) large (6 × 6) Pt(111) supercells; and (d) a complete ion pair of [CCIm][NTf] in a (6 × 6) Pt(111) supercell. Based on DFT, we simulated IR spectra and compared them to the experimental data. Our results suggest that the binding motif and orientation of the IL is strongly dependent on the actual IL coverage. In the monolayer (ML), [NTf] interacts strongly with the metal surface and adopts a specific orientation in which it interacts with the Pt surface via the SO groups. Also the [CCIm] cations adopt a preferential orientation up to coverages of 1 ML. Upon transition to the multilayer region, the specific orientation of the ions is gradually lost.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.1c01900DOI Listing
October 2021

Coaxially Bioprinted Cell-Laden Tubular-Like Structure for Studying Glioma Angiogenesis.

Front Bioeng Biotechnol 2021 1;9:761861. Epub 2021 Oct 1.

Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Glioblastomas are the most frequently diagnosed and one of the most lethal primary brain tumors, and one of their key features is a dysplastic vascular network. However, because the origin of the tumor blood vessels remains controversial, an optimal preclinical tumor model must be established to elucidate the tumor angiogenesis mechanism, especially the role of tumor cells themselves in angiogenesis. Therefore, shell-glioma cell (U118)-red fluorescent protein (RFP)/core-human umbilical vein endothelial cell (HUVEC)-green fluorescent protein (GFP) hydrogel microfibers were coaxially bioprinted. U118-RFP and HUVEC-GFP cells both exhibited good proliferation in a three-dimensional (3D) microenvironment. The secretability of both vascular endothelial growth factor A and basic fibroblast growth factor was remarkably enhanced when both types of cells were cocultured in 3D models. Moreover, U118 cells promoted the vascularization of the surrounding HUVECs by secreting vascular growth factors. More importantly, U118-HUVEC-fused cells were found in U118-RFP/HUVEC-GFP hydrogel microfibers. Most importantly, our results indicated that U118 cells can not only recruit the blood vessels of the surrounding host but also directly transdifferentiate into or fuse with endothelial cells to participate in tumor angiogenesis . The coaxially bioprinted U118-RFP/HUVEC-GFP hydrogel microfiber is a model suitable for mimicking the glioma microenvironment and for investigating tumor angiogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2021.761861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517394PMC
October 2021

Application of the Willis covered stent in the treatment of internal carotid artery blood blister-like aneurysms.

Neurosurg Rev 2021 Oct 18. Epub 2021 Oct 18.

Department of Neurosurgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

The optimal treatment for blood blister-like aneurysm (BBA) has not yet been determined, and BBA has a high recurrence rate after stent-assisted embolization. The purpose of the present study was to evaluate the safety and feasibility of patients with BBA rupture in the acute phase or patients with BBA who have recurrence after stent-assisted coil embolization. Eight patients (8 women, mean age 50.3 ± 3.7 years) who presented with ruptured BBA or recurrence BBA that had been treated by stent-assisted embolization (5 patients after primary treatment of stent-assisted embolization) were retrospectively reviewed. Clinical follow-up was performed at 1 year after endovascular treatment. All patients were successfully treated with the WCS, and immediate postoperative angiography showed that the aneurysms were completely isolated. The ophthalmic artery was covered by WCS in one patient; however, this patient did not show any clinical visual field or vision symptoms. Procedure-related complications such as aneurysm rupture, vasospasm, acute thrombosis, or thromboembolism did not occur in any case. All patients were followed up for 1 year after endovascular treatment, and they were in good condition without recurrence. One patient developed delayed bleeding at the right temporal lobe. All patients had good clinical prognosis (modified Rankin Scale score ≤ 2). WCS implantation may be a safe and feasible strategy for patients with BBA rupture in the acute phase and patients with BBA who have recurrence after stent-assisted coil embolization and is a promising option worth exploring.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10143-021-01666-3DOI Listing
October 2021

Enzyme- and Relative Humidity-Responsive Antimicrobial Fibers for Active Food Packaging.

ACS Appl Mater Interfaces 2021 Oct 14. Epub 2021 Oct 14.

Center for Nanotechnology and Nanotoxicology, Department of Environmental Health, Harvard T. H. Chan School of Public Health, Harvard University, Boston, Massachusetts 02115, United States.

Active food packaging materials that are sustainable, biodegradable, and capable of precise delivery of antimicrobial active ingredients (AIs) are in high demand. Here, we report the development of novel enzyme- and relative humidity (RH)-responsive antimicrobial fibers with an average diameter of 225 ± 50 nm, which can be deposited as a functional layer for packaging materials. Cellulose nanocrystals (CNCs), zein (protein), and starch were electrospun to form multistimuli-responsive fibers that incorporated a cocktail of both free nature-derived antimicrobials such as thyme oil, citric acid, and nisin and cyclodextrin-inclusion complexes (CD-ICs) of thyme oil, sorbic acid, and nisin. The multistimuli-responsive fibers were designed to release the free AIs and CD-ICs of AIs in response to enzyme and RH triggers, respectively. Enzyme-responsive release of free AIs is achieved due to the degradation of selected polymers, forming the backbone of the fibers. For instance, protease enzyme can degrade zein polymer, further accelerating the release of AIs from the fibers. Similarly, RH-responsive release is obtained due to the unique chemical nature of CD-ICs, enabling the release of AIs from the cavity at high RH. The successful synthesis of CD-ICs of AIs and incorporation of antimicrobials in the structure of the multistimuli-responsive fibers were confirmed by X-ray diffraction and Fourier transform infrared spectrometry. Fibers were capable of releasing free AIs when triggered by microorganism-exudated enzymes in a dose-dependent manner and releasing CD-IC form of AIs in response to high relative humidity (95% RH). With 24 h of exposure, stimuli-responsive fibers significantly reduced the populations of foodborne pathogenic bacterial surrogates (by ∼5 log unit) and (by ∼5 log unit), as well as fungi (by >1 log unit). More importantly, the fibers released more AIs at 95% RH than at 50% RH, which resulted in a higher population reduction of at 95% RH. Such biodegradable, nontoxic, and multistimuli-responsive antimicrobial fibers have great potential for broad applications as active and smart packaging systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c12319DOI Listing
October 2021

Vezatin regulates seizures by controlling AMPAR-mediated synaptic activity.

Cell Death Dis 2021 Oct 12;12(10):936. Epub 2021 Oct 12.

Department of Neurology, the Second Affiliated Hospital of Chongqing Medical University, 400010, Chongqing, China.

Although many studies have explored the mechanism of epilepsy, it remains unclear and deserves further investigation. Vezatin has been reported to be a synaptic regulatory protein involved in regulating neuronal synaptic transmission (NST). However, the role of vezatin in epilepsy remains unknown. Therefore, the aims of this study are to investigate the underlying roles of vezatin in epilepsy. In this study, vezatin expression was increased in hippocampal tissues from pilocarpine (PILO)-induced epileptic mice and a Mg-free medium-induced in vitro seizure-like model. Vezatin knockdown suppressed seizure activity in PILO-induced epileptic mice. Mechanistically, vezatin knockdown suppressed AMPAR-mediated synaptic events in epileptic mice and downregulated the surface expression of the AMPAR GluA1 subunit (GluA1). Interestingly, vezatin knockdown decreased the phosphorylation of GluA1 at serine 845 and reduced protein kinase A (PKA) phosphorylation; when PKA phosphorylation was suppressed by H-89 (a selective inhibitor of PKA phosphorylation) in vitro, the effects of vezatin knockdown on reducing the phosphorylation of GluA1 at serine 845 and the surface expression of GluA1 were blocked. Finally, we investigated the pattern of vezatin in brain tissues from patients with temporal lobe epilepsy (TLE), and we found that vezatin expression was also increased in patients with TLE. In summary, the vezatin expression pattern is abnormal in individuals with epilepsy, and vezatin regulates seizure activity by affecting AMPAR-mediated NST and the surface expression of GluA1, which is involved in PKA-mediated phosphorylation of GluA1 at serine 845, indicating that vezatin-mediated regulation of epileptic seizures represents a novel target for epilepsy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-021-04233-2DOI Listing
October 2021

Molecular Analysis of Prognosis and Immune Pathways of Pancreatic Cancer Based on TNF Family Members.

J Oncol 2021 30;2021:2676996. Epub 2021 Sep 30.

Department of Hepatobiliary and Pancreatic Surgery, Zhuzhou Central Hospital, Zhuzhou, China.

Background: Tumor necrosis factor (TNF) family members play a vital role in anticancer therapy. This study aimed to screen the critical markers for the prognostic analysis of pancreatic adenocarcinoma (PAAD) by analyzing the clustering patterns of TNF family members in PAAD.

Methods: In this study, the NMF clustering method was adopted to cluster samples from The Cancer Genome Atlas (TCGA) to acquire the clustering pattern of the TNF family in PAAD. Differential gene analysis was performed according to TNF family gene clusters. The support vector machine (SVM) method was conducted for further gene screening, and the risk score model of the screened genes was constructed by Lasso. The single sample gene set enrichment analysis (ssGSEA) method was adopted for immunoenrichment analysis and tumor immune cycle analysis. Genes associated with risk scores were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis.

Results: We clustered PAAD into two groups based on TNF family genes. Nineteen TNF family genes were significantly associated with the clinical characteristics of PAAD patients. The risk score formula was composed of RHOD, UBE2C, KLHDC7b, MSLN, ADAM8, NME3, GNG2, and MCOLN3. GSE57495 and GSE62452 datasets verified that patients in the high-risk group had a worse prognosis than those in the low-risk group. The risk score-related genes analyzed by GO and KEGG were mainly involved in the modulation of chemical synaptic transmission and synaptic vesicle cycle pathway. There were significant differences in the expression of 15 immune cells between the high-risk group and the low-risk group. The risk score was positively correlated with HCK, interferon, MHC-I, and STAT1. The expression of genes relevant to chemokine, immunostimulator, MHC, and receptor was strongly associated with the risk score.

Conclusion: The risk score model based on the TNF family can predict the prognosis and immune status of PAAD patients. Further research is needed to verify the clinical prognostic value of risk scores.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/2676996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497127PMC
September 2021

Sustainable phosphorus recovery from wastewater and fertilizer production in microbial electrolysis cells using the biochar-based cathode.

Sci Total Environ 2021 Oct 8;807(Pt 2):150881. Epub 2021 Oct 8.

School of Resource and Environmental Sciences, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Wuhan University, No. 129 Luoyu Road, Wuhan 430079, China. Electronic address:

Reducing the energy consumption and electrode cost for electrochemical recovery of phosphorus (P) from wastewater is crucial for the large-scale application. In this study, biochar electrodes were investigated as the low-cost cathode in a microbial electrolysis cell (MEC) and this P-enriched biochar electrode was directly retrieved as P fertilizer after wastewater treatment. The Fe salt modified biochar significantly increased the electrochemical performance of MECs due to the improved electrical conductivity and cathodic activity. Compared to the pristine biochar cathode, the current density of the MEC increased from 16.8 ± 0.2 A/m to 20.7 ± 0.8 A/m, and the P removal increased from 28.8% ± 1% to 62.4% ± 3.5%. The power consumption was 0.25 ± 0.01 kWh/kg P which was more than one order of magnitude lower than the previous report. It was also demonstrated that the P enriched biochar amended soil improved the Pakchoi cultivation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.150881DOI Listing
October 2021

Interaction between Ionic Liquids and a Pt(111) Surface Probed by Coadsorbed CO as a Test Molecule.

J Phys Chem Lett 2021 Oct 8;12(41):10079-10085. Epub 2021 Oct 8.

Interface Research and Catalysis, ECRC, Friedrich-Alexander-Universität Erlangen-Nürnberg, Egerlandstraße 3, 91058 Erlangen, Germany.

We used temperature-programmed infrared reflection absorption spectroscopy (TP-IRAS) to study the desorption behavior of CO on Pt(111) coadsorbed with four kinds of ionic liquids (ILs), namely 1-butyl-1-methyl-pyrrolidinium-bis(trifluoromethylsulfonyl)imide ([CCPyr][NTf]), 1-ethyl-3-methyl-imidazolium-bis(trifluoromethylsulfonyl)imide ([CCIm][NTf]), 1-butyl-1-methyl-pyrrolidinium-trifluoro-methanesulfonate ([CCPyr][OTf]), and 1-butyl-1-methyl-pyrrolidinium-hexafluorophosphate ([CCPyr][PF]). We found that CO desorbs earlier from a Pt(111) surface with coadsorbed ILs than without. In addition, the CO desorption temperature varies between different types of coadsorbed ILs, which follows the order: [CCPyr][PF] (365 K) > [CCPyr][NTf] (362 K) > [CCIm][NTf] (352 K) > [CCPyr][OTf] (348 K). We ascribe the difference in CO desorption temperature to the different interaction strength between ILs and the Pt(111) surface. A stronger IL-Pt(111) interaction leads to a lower CO desorption temperature. We suggest that TP-IRAS experiments of CO coadsorbed with ILs can be a useful method to aid the characterization of the interaction strength between ILs and metal surfaces such as Pt(111).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpclett.1c02983DOI Listing
October 2021

Ridge preservation in maxillary molar extraction sites with severe periodontitis: a prospective observational clinical trial.

Clin Oral Investig 2021 Oct 8. Epub 2021 Oct 8.

Department of Restorative Dentistry, University of Washington, Seattle, WA, USA.

Objectives: To assess alveolar bone changes and treatment modality alterations after ridge preservation on maxillary molar extraction sockets with severe periodontitis, compared to natural healing.

Material And Methods: Thirty-six maxillary infected-molar teeth either receiving ridge preservation (RG group) or undergoing natural healing (NT group) were investigated. Cone-beam computed tomography (CBCT) scanning was performed immediately after surgery (the baseline) and repeated 6 months later to measure the linear and volumetric changes of the sockets.

Results: Based on radiographic measurements, alveolar bone width decreased by 1.58 ± 4.61 mm in the NT group but increased by 3.74 ± 4.17 mm in the RG group (p < 0.05). Significant increases in ridge height at the center of both the NT (7.54 ± 4.54 mm) and RG (9.20 ± 3.26 mm) groups were observed. Mean sinus pneumatization was 0.19 ± 0.45 mm in the RG group and 0.59 ± 0.63 mm in the NT group (p < 0.05). The relative increase in total ridge volume was 8.0% and 35.5% in the NT and RG group, respectively (p < 0.05). Implant placement with additional sinus augmentation procedure was performed in 16.7% of the RG group cases, whereas 50% in the NT group cases.

Conclusions: Ridge preservation in the maxillary molar extraction sockets with severe periodontitis can improve alveolar ridge dimensions and decrease the necessity of advanced regenerative procedures at implant placement compared to natural healing.

Clinical Relevance: Ridge preservation on maxillary molar extraction sockets with severe periodontitis maintained the vertical bone height more efficiently and resulted in less need for sinus augmentation procedures at 6 months compared to natural healing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00784-021-04204-zDOI Listing
October 2021

Total synthesis of bioactive tetracyclic norditerpene dilactones.

Org Biomol Chem 2021 Oct 8. Epub 2021 Oct 8.

Molecular Synthesis Center & Key Laboratory of Marine Drugs, Ministry of Education; School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266071, China.

Tetracyclic norditerpene dilactones are an important class of terpenoids that have been isolated from both terrestrial and marine sources, typically from Podocarpus plants and from filamentous fungi. This class of molecules shares a common 6/6/6/5 tetracyclic ring skeleton, which possesses a densely oxygenated carbon framework and contiguous stereocenters. What's more challenging for synthetic chemists are the consecutive sp-hybridized carbon centers, which exacerbates the strain/rigidity of the whole molecule. In addition, many of these molecules display promising biological activities, such as antitumor, insecticidal, anti-feedant, allelopathic, and antibiotic activities. The unique structures and interesting biological profiles of norditerpene dilactones have attracted considerable attention from synthetic chemists. Herein we summarize the synthetic efforts with respect to tetranorditerpene dilactones.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1ob01535dDOI Listing
October 2021

MicroRNA-142-3p inhibits autophagy and promotes intracellular survival of Mycobacterium tuberculosis by targeting ATG16L1 and ATG4c.

Int Immunopharmacol 2021 Oct 4;101(Pt A):108202. Epub 2021 Oct 4.

Institute of Clinical Laboratory Medicine, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, School of Medical Technology, Guangdong Medical University, Dongguan, 523808, Guangdong, PR China; School of Clinical Medicine, Ningxia Medical University, Yinchuan, 750004, Ningxia, PR China. Electronic address:

Background: Mycobacterium tuberculosis (M. tuberculosis), which parasitizes host macrophages and lead to cellular immunologic responses, such as autophagy and apoptosis. Several studies had indicated that autophagy played important roles in alleviating intracellular survival of M. tuberculosis by accelerating the maturation of phagosome. Previously, we found miR-142-3p was significantly down-regulated in the macrophages after infection with M. tuberculosis. However, the role of miR-142-3p in the regulation of autophagy and M. tuberculosis survival is elusive.

Methods: Bioinformatics analysis was used to obtain target genes of miR-142-3p; the binding sites of ATG16L1 and ATG4c were further confirmed with dual luciferase reporter assay; RAW264.7 cells were infected with H37Ra and the expression of miR-142-3p was measured by qRT-PCR; the autophagic marker protein was detected by western blot as well as immunofluorescence microscopy and transmission electron microscopes analysis.

Results: Overexpression of miR-142-3p significantly inhibited H37Ra-induced activation of autophagy, blocked the maturation of phagosome in macrophages and promoted M. tuberculosis survival in macrophages. Furthermore, miR-142-3p negatively-regulated expression of ATG16L1 and ATG4c by directly targeting its 3'-UTR, and meaningfully abated the level of autophagy.

Conclusion: These findings suggested that miR-142-3p inhibited M. tuberculosis-induced activation of autophagy and promoted H37Ra survival in RAW264.7 cells by targeting ATG16L1 and ATG4c.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2021.108202DOI Listing
October 2021

Long‑chain non‑coding RNA promotes cell autophagy by modulating the miR‑181c‑5p/ and miR‑1192/ axes.

Int J Mol Med 2021 Dec 5;48(6). Epub 2021 Oct 5.

Institute of Clinical Laboratory Medicine, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, School of Medical Technology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.

The main aim of the present study was to explore the role of long‑chain non‑coding RNA (lncRNA) growth arrest‑specific transcript 5 () in macrophage autophagy. Firstly, the expression of lncRNA during cell starvation or following treatment with 3‑methyladenine was determined using reverse transcription‑quantitative PCR (RT‑qPCR). Additionally, fluorescent hybridization (FISH) assay was utilized to determine the localization of the expression of lncRNA in RAW264.7 cells. cell models were established through the transfection of LV5‑lncRNA (LV5‑) or LV3‑shRNA‑lnc (sh‑), in order to overexpress or knockdown lncRNA expression in RAW264.7 cells. The potential target microRNAs (miRNAs/miRs) of lncRNA were analyzed using bioinformatics. The formation of autophagic bodies was detected with the use of laser confocal and transmission electron microscopy. Dual‑luciferase reporter assay was performed to determine the target specificities of miR‑181c‑5p or miR‑1192 to lncRNA and autophagy‑related gene () or . The mRNA levels of miR181c‑5p, miR‑1192, as well as and were detected using RT‑qPCR. The protein levels of microtubule‑associated proteins 1A/1B light chain 3B (LC3), p62, and were measured using western blot analysis. It was revealed that lncRNA expression in RAW264.7 macrophages increased significantly during starvation‑induced autophagy, and that lncRNA overexpression was able to markedly promote the formation of autophagic bodies. Bioinformatics analysis demonstrated that miR‑181c‑5p and miR‑1192 were potential targets of lncRNA , which was further confirmed by RT‑qPCR, western blot analysis and the dual‑luciferase reporter assay. Finally, it was confirmed that lncRNA promoted autophagy by sponging miR‑181c‑5p and miR‑1192, and upregulating the expression levels of the key autophagic regulators, and . On the whole, the present study demonstrates that total, lncRNA promotes macrophage autophagy by targeting the miR‑181c‑5p/ and miR‑1192/ axes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ijmm.2021.5042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510682PMC
December 2021

Iron homeostasis in arthropathies: From pathogenesis to therapeutic potential.

Ageing Res Rev 2021 Oct 1;72:101481. Epub 2021 Oct 1.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address:

Iron is an essential element for proper functioning of cells within mammalian organ systems; in particular, iron homeostasis is critical for joint health. Excess iron can induce oxidative stress damage, associated with the pathogenesis of iron-storage and ageing-related diseases. Therefore, iron levels in body tissues and cells must be tightly regulated. In the past decades, excess iron content within joints has been found in some patients with joint diseases including hemophilic arthropathy, hemochromatosis arthropathy, and osteoarthritis (OA). Currently, increased evidence has shown that iron accumulation is closely associated with multiple pathological changes of these arthropathies. This review summarizes system-level and intracellular regulation of iron homeostasis, and emphasizes the role of iron in synovial alterations, cartilage degeneration, and subchondral bone of several arthropathies. Of note, we discuss the potential link between iron homeostasis and OA pathogenesis. Finally, we discuss the therapeutic potential of maintaining iron homeostasis in these arthropathies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.arr.2021.101481DOI Listing
October 2021

Beyond Mutual Information: Generative Adversarial Network for Domain Adaptation using Information Bottleneck Constraint.

IEEE Trans Med Imaging 2021 Oct 4;PP. Epub 2021 Oct 4.

Medical images from multicentres often suffer from the domain shift problem, which makes the deep learning models trained on one domain usually fail to generalize well to another. One of the potential solutions for the problem is the generative adversarial network (GAN), which has the capacity to translate images between different domains. Nevertheless, the existing GAN-based approaches are prone to fail at preserving image-objects in image-to-image (I2I) translation, which reduces their practicality on domain adaptation tasks. In this regard, a novel GAN (namely IB-GAN) is proposed to preserve image-objects during cross-domain I2I adaptation. Specifically, we integrate the information bottleneck constraint into the typical cycle-consistency-based GAN to discard the superfluous information (e.g., domain information) and maintain the consistency of disentangled content features for image-object preservation. The proposed IB-GAN is evaluated on three tasks-polyp segmentation using colonoscopic images, the segmentation of optic disc and cup in fundus images and the whole heart segmentation using multi-modal volumes. We show that the proposed IB-GAN can generate realistic translated images and remarkably boost the generalization of widely used segmentation networks (e.g., U-Net).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TMI.2021.3117996DOI Listing
October 2021

Achieving the Sustainable Development Goal of Eliminating Preventable Newborn Deaths - China in Action.

Authors:
Tao Xu

China CDC Wkly 2021 Jul;3(31):665-668

National Center for Women and Children's Health, Chinese Center for Disease Control and Prevention, Beijing, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.46234/ccdcw2021168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392906PMC
July 2021

The Trend and Cause of Mortality Burden in Infancy - China, 1990-2019.

Authors:
Chenran Wang Tao Xu

China CDC Wkly 2021 Apr;3(16):340-345

National Center for Women and Children's Health, Chinese Center for Disease Control and Prevention, Beijing, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.46234/ccdcw2021.091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393072PMC
April 2021

Inadequate Nutrition and Associated Factors in Children Aged 6 to 24 Months - 4 Counties, Liangshan Yi Autonomous Prefecture, China, 2018.

China CDC Wkly 2020 Nov;2(45):873-877

National Center for Women and Children's Health, China CDC, Beijing, China.

Symptoms of malnutrition including anemia, stunting, wasting, and being underweight among children remained one of the major public health problems in poorer areas in China. More research is needed to guide interventions to improve nutrition and health among children in low-income regions.



The prevalences of anemia, stunting, wasting, and being underweight were 51.9%, 25.6%, 14.6%, and 9.5%, respectively, among children aged 6 to 24 months in the poorest areas of Liangshan. Associated factors were gender, age, education level and occupation of mother, breastfeeding, and caregiver knowledge.



Improving caregiver knowledge of nutrition and child feeding practices is crucial to address malnutrition among children. These findings can help more precisely understand the child health needs in poorer areas in order to develop effective interventions. They also provide evidence-based information to formulate child health promotion strategies in other countries with similar situations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.46234/ccdcw2020.155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422359PMC
November 2020

Regulation of fruit ripening by the brassinosteroid biosynthetic gene SlCYP90B3 via an ethylene-dependent pathway in tomato.

Hortic Res 2020 Oct 1;7(1):163. Epub 2020 Oct 1.

State Agricultural Ministry Laboratory of Horticultural Crop Growth and Development, Department of Horticulture, Zhejiang University, Hangzhou, 310058, China.

The essential role of ethylene in fruit ripening has been thoroughly studied. However, the involvement of brassinosteroids (BRs) in the regulation of fruit ripening and their relationship with the ethylene pathway are poorly understood. In the current study, we found that BRs were actively synthesized during tomato fruit ripening. We then generated transgenic lines overexpressing or silencing SlCYP90B3, which encodes a cytochrome P450 monooxygenase that catalyzes the rate-limiting step of BR synthesis. The expression level of SlCYP90B3 was positively related to the contents of bioactive BRs as well as the ripening process in tomato fruit, including enhanced softening and increased soluble sugar and flavor volatile contents. Both carotenoid accumulation and ethylene production were strongly correlated with the expression level of SlCYP90B3, corroborated by the altered expression of carotenoid biosynthetic genes as well as ethylene pathway genes in transgenic tomato fruits. However, the application of the ethylene perception inhibitor 1-methycyclopropene (1-MCP) abolished the promotion effect of SlCYP90B3 overexpression on carotenoid accumulation. Taken together, these results increase our understanding of the involvement of SlCYP90B3 in bioactive BR biosynthesis as well as fruit ripening in tomato, thus making SlCYP90B3 a target gene for improvement of visual, nutritional and flavor qualities of tomato fruits with no yield penalty.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41438-020-00383-0DOI Listing
October 2020

The fate of antibiotic resistance genes and their influential factors during excess sludge composting in a full-scale plant.

Bioresour Technol 2021 Sep 27:126049. Epub 2021 Sep 27.

College of Resources, Sichuan Agricultural University, Chengdu 611130, PR China.

The alteration of antibiotic resistance genes (ARGs) during sludge composting has been less studied in a full-scale plant, causing the miss of practical implications for understanding/managing ARGs. Therefore, this study tracked the changes of ARGs and microbial communities in a full-scale plant engaged in excess sludge composting and then explored the key factors regulating ARGs through a series of analyses. After composting, the absolute and relative abundance of ARGs decreased by 91.90% and 66.57%, respectively. Additionally, PLS-PM analysis showed that MGEs, composting physicochemical properties were the most vital factors directly influencing ARGs. Finally, network analysis indicated that Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were the main hosts of ARGs. Based on these findings, it can be known that full-scale composting could reduce ARGs risk to an extent.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2021.126049DOI Listing
September 2021
-->