Publications by authors named "Tao Song"

683 Publications

Commentary: Heparin Attenuates Histone-Mediated Cytotoxicity in Septic Acute Kidney Injury.

Front Med (Lausanne) 2021 20;8:647741. Epub 2021 Apr 20.

Department of Nephrology of West China Hospital, Institute for Disaster Management and Reconstruction, Sichuan University, Chengdu, China.

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http://dx.doi.org/10.3389/fmed.2021.647741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093565PMC
April 2021

observation of the crystal structure transition of Pt-Sn intermetallic nanoparticles during deactivation and regeneration.

Chem Commun (Camb) 2021 May 6. Epub 2021 May 6.

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.

The mechanism of the deactivation and regeneration of PtSn intermetallic compound nanoparticle (iNP) catalysts was studied by in situ TEM investigation. Our study reveals the reversible dynamic structural transition of the iNPs during deactivation and regeneration, which provides a direct correlation between the atomic structure and the catalytic activity of the iNPs.
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http://dx.doi.org/10.1039/d1cc01181bDOI Listing
May 2021

Study on the Appropriate Timing of Postoperative Adaptive Radiotherapy for High-Grade Glioma.

Cancer Manag Res 2021 28;13:3561-3572. Epub 2021 Apr 28.

Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

Purpose: To investigate the appropriate timing of adaptive radiotherapy (ART) for high-grade glioma.

Methods: Ten patients with high-grade gliomas were selected and underwent CT/MRI (CT/MRI, CT/MRI, CT/MRI, and CT/MRI) scans before RT and during 10-, 20- and 30-fraction RT, and the corresponding RT plans (plan, plan, plan and plan) were made. The dose of the initial plan (plan) was projected to CT2 and CT3 using the image registration technique to obtain the projection plans (plan and plan) and by superimposing the doses to obtain the ART plans (plan and plan), respectively. The dosimetric differences in the target volume and organs at risk (OARs) were compared between the projection and adaptive plans. The tumor control probability (TCP) for the planning target volume (PTV) and normal tissue complication probability (NTCP) for the OARs were compared between the two adaptive plans.

Results: Compared with the projection plan, the D to the PTV of ART decreased, the conformity index (CI) to the PTV increased, and the D/D to the brainstem, optic chiasm and pituitary, as well as the V, V, V and V to the normal brain decreased. The D to the pituitary and optic chiasm as well as the V, V, V and V to the normal brain in plan were lower than those in plan, while the CI to the PTV was higher than that in plan. The TCP of the PTV in plan was higher than that in plan.

Conclusion: ART can improve the precision of target volume irradiation and reduce the irradiation dose to the OARs in high-grade glioma. The time point after 10 fractions of RT is appropriate for ART.
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http://dx.doi.org/10.2147/CMAR.S300094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8089024PMC
April 2021

The Clinical Efficacy of High-Voltage Long-Duration Pulsed Radiofrequency Treatment in Pudendal Neuralgia: A Retrospective Study.

Neuromodulation 2021 May 4. Epub 2021 May 4.

Department of Pain Medicine, the First Affiliated Hospital, China Medical University, Shenyang, China.

Background: Patients with pudendal neuralgia (PN) experience long-lasting chronic pain, hyperalgesia, and comorbid emotional disorders, such as depression and anxiety. Treatment via conventional pulsed radiofrequency (PRF) current carries a significantly high rate of failure.

Objective: To determine the safety and clinical efficacy of high-voltage, long-duration PRF application to the pudendal nerve in patients with PN.

Study Design: Observational retrospective design, self before-after controlled clinical trial.

Materials And Methods: We analyzed the records of 70 patients of our hospital with diagnosed PN. Treatment consisted of PRF application to the pudendal nerve, using computed tomography guidance to target the pudendal nerve at the level of the ischial spine or ischial tuberosity of the affected side. PRF was applied with the following parameters: temperature 42°C, frequency 2 Hz, pulse width 20 ms, field intensity ramped gradually from 40 to 90 V, duration 900 sec. The therapeutic effect was evaluated by collecting patient scores for the visual analog scale (VAS), SF-36 health survey questionnaire (SF-36), and patient health questionnaire (PHQ-9) before treatment and at 1-, 4-, and 12-week follow-ups after PRF treatment. Data were analyzed by paired t-test with p < 0.05 considered to be statistically significant.

Results: VAS, SF-36, and PHQ-9 scores at 1, 4, and 12 weeks after high-voltage long-duration PRF treatment were significantly improved relative to their respective pretreatment baseline scores (p < 0.05 for all). The effective rate at 12 weeks after high-voltage long-duration PRF was up to 88.6%.

Limitations: A small sample size and lack of a control group.

Conclusions: High-voltage long-duration PRF provided significant short-term (at least 12 weeks) pain relief to most patients with PN; it also improved subjective measures of depression and quality of life over the same duration of time.
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http://dx.doi.org/10.1111/ner.13401DOI Listing
May 2021

A correlation study of ischiocavernosus muscle injury with different types of pelvic fractures and erectile dysfunction after pelvic fracture.

OTA Int 2020 Dec 16;3(4):e081. Epub 2020 Sep 16.

Department of Urology, Beijing Shijitan Hospital, Capital Medical University, Beijing.

Objective: To explore the correlation between ischiocavernosus muscle injury (ICMI) with different types of pelvic fractures and erectile dysfunction (ED) after pelvic fracture.

Design: Retrospective analysis of a prospective database.

Setting: The study was carried out at the affiliated hospital of Zunyi Medical University.

Patients/participants: A total of 776 male patients with pelvic fracture, aged 18 to 67 years, were recruited for this study by retrospective analysis, and based on the diagnosis of ED and the presence of ICMI, the participants were divided into ED and non-ED groups as well as ICMI and non-ICMI groups.

Intervention: No.

Main Outcome Measurements: ICMI, the type of pelvic fracture, International Index of Erectile Function-5 scores. Computed tomography/magnetic resonance imaging scans, electromyography (motor unit potential) was used to diagnose ICMI.

Results: The International Index of Erectile Function-5 score was 19.7 ± 5.9. The incidence of ED was 27.3%, the duration time of ED was 30 ± 23 months, and the incidence of reversible ED was 39.6% and of irreversible ED was 60.4%. The incidence of ICMI was 29.4%, among which the incidence of unilateral injury was 57.9%, and the incidence of bilateral injury was 42.1%. Among all pelvic fractures, the incidence of pubic ramus fracture was 88.1%. Bilateral pubic ramus fractures, bilateral fractures of the ischial ramus, and ICMI were independent risk factors for ED after pelvic fracture. Bilateral pubic ramus fractures and pubic symphysis separation were independent risk factors for ICMI. Unilateral ICMI was an independent risk factor for reversible ED, while bilateral ICMI was an independent risk factor for irreversible ED.

Conclusions: ICMI is associated with ED and may be a cause for ED, while pubic ramus fracture, ischial ramus fracture, and pubic symphysis separation may be the main causes of ICMI. Unilateral ICMI may be the main risk factor for transient ED, and bilateral ICMI may be the main risk factor for permanent ED.
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http://dx.doi.org/10.1097/OI9.0000000000000081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022899PMC
December 2020

CSConv2d: A 2-D Structural Convolution Neural Network with a Channel and Spatial Attention Mechanism for Protein-Ligand Binding Affinity Prediction.

Biomolecules 2021 Apr 27;11(5). Epub 2021 Apr 27.

College of Computer Science and Technology, China University of Petroleum, Qingdao 266580, China.

The binding affinity of small molecules to receptor proteins is essential to drug discovery and drug repositioning. Chemical methods are often time-consuming and costly, and models for calculating the binding affinity are imperative. In this study, we propose a novel deep learning method, namely CSConv2d, for protein-ligand interactions' prediction. The proposed method is improved by a DEEPScreen model using 2-D structural representations of compounds as input. Furthermore, a channel and spatial attention mechanism (CS) is added in feature abstractions. Data experiments conducted on ChEMBLv23 datasets show that CSConv2d performs better than the original DEEPScreen model in predicting protein-ligand binding affinity, as well as some state-of-the-art DTIs (drug-target interactions) prediction methods including DeepConv-DTI, CPI-Prediction, CPI-Prediction+CS, DeepGS and DeepGS+CS. In practice, the docking results of protein (PDB ID: 5ceo) and ligand (Chemical ID: 50D) and a series of kinase inhibitors are operated to verify the robustness.
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http://dx.doi.org/10.3390/biom11050643DOI Listing
April 2021

Usefulness of New Shear Wave Elastography Technique for Noninvasive Assessment of Liver Fibrosis in Patients with Chronic Hepatitis B: A Prospective Multicenter Study.

Ultraschall Med 2021 Apr 28. Epub 2021 Apr 28.

Department of Ultrasound, Guangdong Key Laboratory of Liver Disease Research, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Purpose:  To explore the usefulness of liver stiffness measurements (LSMs) by sound touch elastography (STE) and sound touch quantification (STQ) in chronic hepatitis B (CHB) patients for staging fibrosis.

Methods:  This prospective multicenter study recruited normal volunteers and CHB patients between May 2018 and October 2019. The volunteers underwent LSM by STE and supersonic shear imaging (SSI) or by STQ and acoustic radiation force impulse imaging (ARFI). CHB patients underwent liver biopsy and LSM by both STE/STQ. The areas under the receiver operating characteristic curves (AUCs) for staging fibrosis were calculated.

Results:  Overall, 97 volunteers and 524 CHB patients were finally eligible for the study. The successful STE and STQ measurement rates were both 100 % in volunteers and 99.4 % in CHB patients. The intraclass correlation coefficients (ICCs) for the intra-observer stability of STE and STQ (0.94; 0.90) were similar to those of SSI and ARFI (0.95; 0.87), respectively. STE and STQ showed better accuracy than the aspartate aminotransferase-to-platelet ratio index (APRI) and fibrosis-4 index (FIB-4) (AUC: 0.87 vs 0.86 vs 0.73 vs 0.77) in staging cirrhosis. However, both STE and STQ were not superior to APRI and FIB-4 in staging significant fibrosis (AUC: 0.76 vs 0.73 vs 0.70 vs 0.71, all P-values > 0.05).

Conclusion:  STE and STQ are convenient techniques with a reliable LSM value. They have a similar diagnostic performance and are superior to serum biomarkers in staging cirrhosis in CHB patients.
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http://dx.doi.org/10.1055/a-1376-6734DOI Listing
April 2021

Sirt2 in the Spinal Cord Regulates Chronic Neuropathic Pain Through Nrf2-Mediated Oxidative Stress Pathway in Rats.

Front Pharmacol 2021 9;12:646477. Epub 2021 Apr 9.

Department of Pain Medicine, The First Hospital of China Medical University, Shenyang, China.

Reduction in Nrf2-mediated antioxidant response in the central nervous system plays an important role in the development and maintenance of neuropathic pain (NP). However, the mechanisms regulating Nrf2 activity in NP remain unclear. A recent study revealed that Sirt2, a member of the sirtuin family of proteins, affects antioxidant capacity by modulating Nrf2 activity. Here we examined whether central Sirt2 regulates NP through Nrf2-mediated oxidative stress pathway. In a rat model of spared nerve injury (SNI)-induced NP, mechanical allodynia and thermal hyperalgesia were observed on day 1 and up to day 14 post-SNI. The expression of Sirt2, Nrf2 and its target gene NQO1 in the spinal cord in SNI rats, compared with sham rats, was significantly decreased from day 7 and remained lower until the end of the experiment (day 14). The mechanical allodynia and thermal hyperalgesia in SNI rats were ameliorated by intrathecal injection of Nrf2 agonist tBHQ, which normalized expression of Nrf2 and NQO1 and reversed SNI-induced decrease in antioxidant enzyme superoxide dismutase (SOD) and increase in oxidative stress marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the spinal cord. Moreover, intrathecal injection of a recombinant adenovirus expressing Sirt2 (Ad-Sirt2) that upregulated expression of Sirt2, restored expression of Nrf2 and NQO1 and attenuated oxidative stress in the spinal cord, leading to improvement of thermal hyperalgesia and mechanical allodynia in SNI rats. These findings suggest that peripheral nerve injury downregulates Sirt2 expression in the spinal cord, which inhibits Nrf2 activity, leading to increased oxidative stress and the development of chronic NP.
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http://dx.doi.org/10.3389/fphar.2021.646477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063033PMC
April 2021

Analysis of Resibufogenin on Cardiac conduction reveals a species difference in the cardiac electrophysiology: Rats versus guinea pigs.

Biomed Pharmacother 2021 Apr 23;139:111581. Epub 2021 Apr 23.

National Key Laboratory of Collateral Disease Research and Innovative Chinese Medicine, No.238, the South of Tianshan street, Shijiazhuang 050035, Hebei, China; Shijiazhuang Compound Traditional Chinese Medicine Technology Innovation Center, No.238, the South of Tianshan street, Shijiazhuang 050035, Hebei, China; College of Integrated Traditional Chinese and Western Medicine, Hebei Medical University of Chinese Medicine, No.3, Xingyuan Road, Shijiazhuang 050200, Hebei, China. Electronic address:

Resibufogenin (RBG) is a chemical ingredient of Chan Su. In our research, we found RBG affected cardiac rhythm in a negative chronotropic way in vivo. The cardiac Mapping system ex vivo and the patch clamp in vitro were used to explore how RBG influenced the cardiac electrophysiological properties. The negative chronotropic action of RBG at 100 μM might be attribute to prolongation in the atrioventricular conduction time and reduction in the ventricular conduction velocity. Using whole-cell patch clamp in ventricular myocytes of adult rats, we found that RBG prolonged the action potential duration (APD) in APD, APD, and APD at 100 μM and inhibited calcium currents (I), total outward potassium currents (I), and transient outward potassium current (I) in a concentration-dependent manner, but not on the inward rectifying potassium current (I). Notably, RBG had a potent proarrhythmic action ex vivo in the isolated perfused guinea pig hearts at 10 μM, but not in rats. To avoid the potential cardiotoxicity derived from the distributional differences of ion channels among species, the effect of RGB on I in hERG-HEK293 cells was detected. The IC of RGB on I was more than 100 μM. In summary, all these results indicated that the negative chronotropic action of RBG relied on the blocking activities on multiple ion channels, and the species-difference of proarrhythmic effects might result from lack of the I on the myocardial membrane of guinea pigs. Anyhow, the cardiotoxicity observed in guinea pigs required further detailed studies to mitigate the potential risks in the clinical application of Chan Su.
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http://dx.doi.org/10.1016/j.biopha.2021.111581DOI Listing
April 2021

Sulfatase 2-Induced Cancer-Associated Fibroblasts Promote Hepatocellular Carcinoma Progression via Inhibition of Apoptosis and Induction of Epithelial-to-Mesenchymal Transition.

Front Cell Dev Biol 2021 6;9:631931. Epub 2021 Apr 6.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: Sulfatase 2 (SULF2) removes the 6--sulfate groups from heparan sulfate proteoglycans (HSPG) and consequently alters the binding sites for various signaling molecules. Here, we elucidated the role of SULF2 in the differentiation of hepatic stellate cells (HSCs) into carcinoma-associated fibroblasts (CAFs) in the hepatocellular carcinoma (HCC) microenvironment and the mechanism underlying CAF-mediated HCC growth.

Methods: The clinical relevance of SULF2 and CAFs was examined using and immunohistochemical (IHC) analyses. Functional studies were performed to evaluate the role of SULF2 in the differentiation of HSCs into CAFs and elucidate the mechanism underlying CAF-mediated HCC growth. Mechanistic studies were performed using the chromatin immunoprecipitation, luciferase reporter, and RNA immunoprecipitation assays. The findings were verified using the nude HCC xenograft mouse model.

Results: The Cancer Genome Atlas (TCGA) database and IHC analyses revealed that the expression of CAF markers, which was positively correlated with that of SULF2 in the HCC tissues, predicted unfavorable postsurgical outcomes. Co-culturing HSCs with HCC cells expressing SULF2 promoted CAF differentiation. Additionally, CAFs repressed HCC cell apoptosis by activating the SDF-1/CXCR4/PI3K/AKT signaling pathway. Meanwhile, SULF2-induced CAFs promoted epithelial-to-mesenchymal transition (EMT) of HCC cells by modulating the SDF-1/CXCR4/OIP5-AS1/miR-153-3p/SNAI1 axis. Studies using HCC xenograft mouse models demonstrated that OIP5-AS1 induced EMT by upregulating SNAI1 and promoted HCC growth .

Conclusion: These data indicated that SULF2 secreted by the HCC cells induced the differentiation of HSCs into CAFs through the TGFβ1/SMAD3 signaling pathway. SULF2-induced CAFs attenuated HCC apoptosis by activating the SDF-1/CXCR4/PI3K/AKT signaling pathway and induced EMT through the SDF-1/CXCR4/OIP5-AS1/miR-153-3p/SNAI1 axis. This study revealed a novel mechanism involved in the crosstalk between HCC cells and CAFs in the tumor microenvironment, which can aid in the development of novel and efficient therapeutic strategies for primary liver cancer.
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http://dx.doi.org/10.3389/fcell.2021.631931DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056031PMC
April 2021

Genomic analysis and functional characterization of immune genes from the RIG-I- and MAVS-mediated antiviral signaling pathway in lamprey.

Genomics 2021 Apr 19. Epub 2021 Apr 19.

College of Life Sciences, Liaoning Normal University, Dalian 116081, China; Lamprey Research Center, Liaoning Normal University, Dalian 116081, China. Electronic address:

Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) are well-known viral RNA sensors in the cytoplasm. RIG-I-mediated antiviral signals are activated by interacting with the adapter protein mitochondrial antiviral signaling (MAVS), which triggers interferon (IFN) responses via a signaling cascade. Although the complete RIG-I receptor signaling pathway has been traced back to teleosts, definitive evidence of its presence in lampreys is lacking. Here, we identified 13 pivotal molecules in the RIG-I signaling pathway in lamprey, and demonstrated that the original RIG-I/MAVS signaling pathway was activated and mediated the expression of unique immunity factors such as RRP4, to inhibit viral proliferation after viral infection in vivo and in vitro. This study confirmed the conservation of the RIG-I pathway, and the uniqueness of the RRP4 effector molecule in lamprey, and further clarified the evolutionary process of the RIG-I antiviral signaling pathway, providing evidence on the origins of innate antiviral immunity in vertebrates.
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http://dx.doi.org/10.1016/j.ygeno.2021.04.030DOI Listing
April 2021

Synergistic Effect of Dielectric Property and Energy Transfer on Charge Separation in Non-Fullerene-Based Solar Cells.

Angew Chem Int Ed Engl 2021 Apr 19. Epub 2021 Apr 19.

Soochow University, Institute of Functional Nano & Soft Materials (FUNSOM), 199 Ren'ai Road, Suzhou, 215123, Suzhou, CHINA.

In non-fullerene-based photovoltaic devices, it still remains unclear how excitons efficiently dissociate into free charge carriers even under a tiny driving force (tens of meV) with reduced energy loss. Here, dielectric constants of different non-fullerene-based solar cells consisting of fluorinated-thienyl benzodithiophene (BDT-2F)-based polymer PM6 as the typical donor, and a selected series of non-fullerene acceptors were precisely measured by a newly developed method. It was found that most of the non-fullerene acceptors exhibited higher dielectric constants than fullerene derivatives (PC61BM and PC71BM). The corresponding photoactive films exhibited not only higher dielectric constants but also the larger dielectric constant differences between donor and non-fullerene acceptors. These would result in lower exciton binding energy and increased charge dissociation possibility with low geminate losses. Additionally, the overlap between the emission spectrum of donor and absorption spectra of non-fullerene acceptors would allow the resonance energy transfer from donor to the acceptor in these non-fullerene-based devices, which was confirmed by investigating the emission spectra of pristine donor (and acceptor) films and corresponding blend films. Such an energy transfer process enhanced the efficient exciton diffusion, promising improved device performance. Therefore, based on the synergistic effect of higher dielectric property and energy transfer on charge separation of selected non-fullerene-based photovoltaic devices, these results provided strong hints to interpret efficient charge separation for the high device performance with a tiny driving force. Our work paves another path to elucidate the intrinsic physical working mechanism on non-fullerene organic solar cells.
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http://dx.doi.org/10.1002/anie.202103357DOI Listing
April 2021

Circulating Histones in Sepsis: Potential Outcome Predictors and Therapeutic Targets.

Front Immunol 2021 24;12:650184. Epub 2021 Mar 24.

Department of Nephrology of West China Hospital, Institute for Disaster Management and Reconstruction, Sichuan University, Chengdu, China.

Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection and is associated with high morbidity and mortality. Circulating histones (CHs), a group of damage-associated molecular pattern molecules mainly derived from neutrophil extracellular traps, play a crucial role in sepsis by mediating inflammation response, organ injury and death through Toll-like receptors or inflammasome pathways. Herein, we first elucidate the molecular mechanisms of histone-induced inflammation amplification, endothelium injury and cascade coagulation activation, and discuss the close correlation between elevated level of CHs and disease severity as well as mortality in patients with sepsis. Furthermore, current state-of-the-art on anti-histone therapy with antibodies, histone-binding proteins (namely recombinant thrombomodulin and activated protein C), and heparin is summarized to propose promising approaches for sepsis treatment.
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http://dx.doi.org/10.3389/fimmu.2021.650184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044749PMC
March 2021

The Feasibility and Positive Effects of Wuqinxi Exercise on the Cognitive and Motor Functions of Patients with Parkinson's Disease: A Pilot Study.

Evid Based Complement Alternat Med 2021 29;2021:8833736. Epub 2021 Mar 29.

School of Martial Arts, Shanghai University of Sport, Shanghai, China.

Introduction: Parkinson's disease (PD) is a chronic degenerative disease of the central nervous system common in middle-aged and elderly people, which has a serious impact on patients' cognitive and motor functions. Exercise can improve the nonmotor symptoms of PD patients, but the optimal type of exercise for the cognitive function of patients is unclear. Therefore, the purpose of this study is the impact of 12 weeks of Wuqinxi exercise on the cognitive and motor function in PD patients.

Methods: Thirty PD patients participated in the study and were randomly assigned to two groups: Wuqinxi group ( = 15) or stretching group ( = 15). All the participants performed a 12-week exercise program twice a week, 90 min/session. The assessments were conducted before and after exercise intervention, included cognitive function (frontal assessment battery (FAB); Stroop test I and II), motor functions (Unified Parkinson's Disease Rating Scale Part III (UPDRS-III); timed up and go (TUG)).

Results: We found the FAB and Stroop I scores were significantly higher in the Wuqinxi group than in the stretching group. Participants in the Wuqinxi group significantly improved their UPDRS-III (17.73 ± 9.88) and TUG (10.50 ± 1.79) score after 12 weeks of training intervention.

Conclusion: The results show that the use of Wuqinxi for rehabilitation therapy for cognition is feasible, widely accepted, and effective in patients with Parkinson's disease. This study provides preliminary evidence for further large-scale and controlled studies.
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http://dx.doi.org/10.1155/2021/8833736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024081PMC
March 2021

Expert consensus of Chinese Association for the Study of Pain on the radiofrequency therapy technology in the Department of Pain.

World J Clin Cases 2021 Mar;9(9):2123-2135

Department of Algology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

On the basis of continuous improvement in recent years, radiofrequency therapy technology has been widely developed, and has become an effective method for the treatment of various intractable pain. Radiofrequency therapy is a technique that uses special equipment and puncture needles to output ultra-high frequency radio waves and accurately act on local tissues. In order to standardize the application of radiofrequency technology in the treatment of painful diseases, Chinese Association for the Study of Pain (CASP) has developed a consensus proposed by many domestic experts and scholars.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017496PMC
March 2021

Expert consensus of the Chinese Association for the Study of Pain on ion channel drugs for neuropathic pain.

World J Clin Cases 2021 Mar;9(9):2100-2109

Department of Algology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

Neuropathic pain (NPP) is a kind of pain caused by disease or damage impacting the somatosensory system. Ion channel drugs are the main treatment for NPP; however, their irregular usage leads to unsatisfactory pain relief. To regulate the treatment of NPP with ion channel drugs in clinical practice, the Chinese Association for the Study of Pain organized first-line pain management experts from China to write an expert consensus as the reference for the use of ion channels drugs . Here, we reviewed the mechanism and characteristics of sodium and calcium channel drugs, and developed recommendations for the therapeutic principles and clinical practice for carbamazepine, oxcarbazepine, lidocaine, bulleyaconitine A, pregabalin, and gabapentin. We hope this guideline provides guidance to clinicians and patients on the use of ion channel drugs for the management of NPP.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017507PMC
March 2021

Chinese Association for the Study of Pain: Expert consensus on chronic postsurgical pain.

World J Clin Cases 2021 Mar;9(9):2090-2099

Department of Algology, The Affiliated T.C.M Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.

Chronic postsurgical pain is a common surgical complication that severely reduces a patient's quality of life. Many perioperative interventions and management strategies have been developed for reducing and managing chronic postsurgical pain. Under the leadership of the Chinese Association for the Study of Pain, an editorial committee was formed for chronic postsurgical pain diagnosis and treatment by experts in relevant fields. The editorial committee composed the main content and framework of this consensus and established a working group. The working group conducted literature review (1989-2020) using key words such as "surgery", "post-surgical", "post-operative", "pain", "chronic", and "persistent" in different databases including MEDLINE, EMBASE, PubMed, Cochrane Central Register of Controlled Trials and Cochrane Database of Systematic Reviews. Only publications in the English language were included. The types of literature included systematic reviews, randomized controlled studies, cohort studies and case reports. This consensus was written based on clinical practice combined with literature evidence. The first draft of the consensus was rigorously reviewed and edited by all the editorial committee experts before being finalized. The level of evidence was assessed by methodological experts based on the Oxford Centre for Evidence-Based Medicine Levels of Evidence. The strength of recommendation was evaluated by all editorial committee experts, and the opinions of most experts were adopted as the final decision. The recommendation level "strong" generally refers to recommendations based on high-level evidence and consistency between clinical behavior and expected results. The recommendation level "weak" generally refers to the uncertainty between clinical behavior and expected results based on low-level evidence.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017506PMC
March 2021

Expert consensus on the diagnosis and treatment of myofascial pain syndrome.

World J Clin Cases 2021 Mar;9(9):2077-2089

Department of Algology, The Third Xiangya Hospital of Central South University, Changsha 410001, Hunan Province, China.

Myofascial pain syndrome (MPS) is characterized by myofascial trigger points and fascial constrictions. At present, domestic and foreign scholars have not reached a consensus on the etiology and pathogenesis of MPS. Due to the lack of specific laboratory indicators and imaging evidence, there is no unified diagnostic criteria for MPS, making it easy to confuse with other diseases. The Chinese Association for the Study of Pain organized domestic experts to formulate this Chinese Pain Specialist Consensus on the diagnosis and treatment of MPS. This article reviews relevant domestic and foreign literature on the definition, epidemiology, pathogenesis, clinical manifestation, diagnostic criteria and treatments of MPS. The consensus is intended to normalize the diagnosis and treatment of MPS and be used by first-line doctors, including pain physicians to manage patients with MPS.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017503PMC
March 2021

Expert consensus of Chinese Association for the Study of Pain on the non-opioid analgesics for chronic musculoskeletal pain.

World J Clin Cases 2021 Mar;9(9):2068-2076

Department of Algology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang Province, China.

Chronic musculoskeletal pain (CMP) is a common occurrence in clinical practice and there are a variety of options for the treatment of it. However, the pharmacological therapy is still considered to be a primary treatment. The recent years have witnessed the emergence of opioid crisis, yet there are no relevant guidelines on how to treat CMP with non-opioid analgesics properly. The Chinese Medical Association for the Study of Pain convened a panel meeting to develop clinical practice consensus for the treatment of CMP with non-opioid analgesics. The purpose of this consensus is to present the application of nonsteroidal anti-inflammatory drugs, serotonin norepinephrine reuptake inhibitors, serotonin and norepinephrine reuptake inhibitors, muscle relaxants, ion channel drugs and topical drugs in CMP.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017502PMC
March 2021

Expert consensus of Chinese Association for the Study of Pain on the application of ozone therapy in pain medicine.

World J Clin Cases 2021 Mar;9(9):2037-2046

Department of Algology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

This consensus was compiled by first-line clinical experts in the field of pain medicine and was organized by the Chinese Association for the Study of Pain. To reach this consensus, we consulted a wide range of opinions and conducted in-depth discussions on the mechanism, indications, contraindications, operational specifications and adverse reactions of ozone iatrotechnique in the treatment of pain disorders. We also referred to related previous preclinical and clinical studies published in recent years worldwide. The purpose of this consensus is to standardize the rational application of ozone iatrotechnique in pain treatment, to improve its efficacy and safety and to reduce and prevent adverse reactions and complications in this process.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017497PMC
March 2021

Expert panel's guideline on cervicogenic headache: The Chinese Association for the Study of Pain recommendation.

World J Clin Cases 2021 Mar;9(9):2027-2036

Department of Algology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

Cervicogenic headache (CEH) has been recognized as a unique category of headache that can be difficult to diagnose and treat. In China, CEH patients are managed by many different specialties, and the treatment plans remain controversial. Therefore, there is a great need for comprehensive evidence-based Chinese experts' recommendations for the management of CEH. The Chinese Association for the Study of Pain asked an expert panel to develop recommendations for a series of questions that are essential for daily clinical management of patients with CEH. A group of multidisciplinary Chinese Association for the Study of Pain experts identified the clinically relevant topics in CEH. A systematic review of the literature was performed, and evidence supporting the benefits and harms for the management of CEH was summarized. Twenty-four recommendations were finally developed through expert consensus voting for evidence quality and recommendation strength. We hope this guideline provides direction for clinicians and patients making treatment decisions for the management of CEH.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017501PMC
March 2021

Asymmetric Charged Conductive Porous Films for Electricity Generation from Water Droplets Capillary Infiltrating.

ACS Appl Mater Interfaces 2021 Apr 12;13(15):17902-17909. Epub 2021 Apr 12.

Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123, China.

Hydrovoltaic devices are proposed as an alternative way to directly generate electricity due to the ubiquity of water and its interaction with specific porous structures. At present, the output power density of the reported device is limited by its low current density arising from the low surface charge density and inferior charge transport capability of the active materials. In this work, an asymmetric structure consisting of positively charged conductive polyaniline (PANI) and negatively charged TiCT MXene is proposed to build a hydrovoltaic device to achieve high conductivity and surface charge density simultaneously. An extra polyvinyl alcohol layer is utilized between PANI and MXene to reserve the asymmetric structure and maintain a constant voltage output. As a result, a peak current density of 1.8 mA/cm is achieved, which is 18 times higher than the previous peak current density of the device with an inert electrode. Our work of incorporating an asymmetric structure provides an alternative way to target high-efficiency hydrovoltaic devices with a large current density.
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http://dx.doi.org/10.1021/acsami.0c21935DOI Listing
April 2021

Bioinspired Hierarchical Nanofabric Electrode for Silicon Hydrovoltaic Device with Record Power Output.

ACS Nano 2021 Apr 9;15(4):7472-7481. Epub 2021 Apr 9.

Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123, China.

Direct electricity generation from water flow/evaporation, coined hydrovoltaic effect, has recently attracted intense interest as a facile approach to harvest green energy from ubiquitous capillary water flow or evaporation. However, the current hydrovoltaic device is inferior in output power efficiency compared to other renewable energy devices. Slow water evaporation rate and inefficient charge collection at device electrodes are two fundamental drawbacks limiting energy output efficiency. Here, we report a bioinspired hierarchical porous fabric electrode that enables high water evaporation rate, efficient charge collection, and rapid charge transport in nanostructured silicon-based hydrovoltaic devices. Such an electrode can efficiently collect charges generated in nanostructured silicon as well as induce a prompt water evaporation rate. At room temperature, the device can generate an open-circuit voltage () of 550 mV and a short-current density () of 22 μA·cm. It can output a power density over 10 μW·cm, which is 3 orders of magnitude larger than all those reported for analogous hydrovoltaic devices. Our results could supply an effective strategy for the development of high-performance hydrovoltaic devices through optimizing electrode structures.
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http://dx.doi.org/10.1021/acsnano.1c00891DOI Listing
April 2021

Three-Dimensional Computed Tomography Reconstruction and Measurement of Nasal End Deformity in Complete Unilateral Cleft Lip and Palate.

Ann Plast Surg 2021 Apr 5. Epub 2021 Apr 5.

From the Center for Cleft Lip and Palate Treatment, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing China.

Background: The repair of nasal deformities secondary to cleft lip and palate is complex and requires reliable preoperative nasal 3-dimensional assessment. This study explored nasal end (defined as the lower third of the external nasal and vestibular parts of the nasal cavity) deformities secondary to unilateral complete cleft lip and palate.

Methods: Three-dimensional nasal end morphometric measurements were obtained from 48 patients who had undergone Millard cleft lip repair and reached skeletal maturity (cleft group) and from 36 age- and ethnicity-matched normal subjects (control group). For the cleft group, paired t tests and 1-way analysis of covariance were used to evaluate the internal and external morphological characteristics of the cleft and noncleft sides of the nasal end, and correlation analysis was done to evaluate the relationship between cleft-side measurements.

Results: In the cleft group, the cleft side showed significantly smaller nasal vestibular volume and skin area, nostril area, nasal column length, and nostril height and greater nostril base length and nasal alar length than the noncleft side (all P < 0.05). Controlling for sex, there were significant differences in the nasal vestibular volume and skin area, internal nasal valve area, long nostril diameter, nostril base length, columella length, nostril height, and nasal alar length between the cleft and control groups (all P < 0.05). On the cleft side, the area of the skin lining of the nasal vestibule positively correlated with the alar length (r = 0.67, P < 0.05).

Conclusions: Three-dimensional nasal end reconstruction provides a more detailed preoperative nasal end morphological evaluation than previously available techniques.

Level Of Evidence: Level III, case-control study.
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http://dx.doi.org/10.1097/SAP.0000000000002837DOI Listing
April 2021

Cobalt-Catalyzed Markovnikov-Selective Radical Hydroacylation of Unactivated Alkenes with Acylphosphonates.

J Am Chem Soc 2021 Apr 30;143(13):4955-4961. Epub 2021 Mar 30.

School of Materials and Chemical Engineering, Ningbo University of Technology, No. 201 Fenghua Road, Ningbo 315211, China.

Acylphosphonates having the 5,5-dimethyl-1,3,2-dioxophosphinanyl skeleton are developed as efficient intermolecular radical acylation reagents, which enable the cobalt-catalyzed Markovnikov hydroacylation of unactivated alkenes at room temperature under mild conditions. The protocol exhibits broad substrate scope and wide functional group compatibility, providing branched ketones in satisfactory yields. A mechanism involving the Co-H mediated hydrogen atom transfer and subsequent trapping of alkyl radicals by acylphosphonates is proposed.
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http://dx.doi.org/10.1021/jacs.1c02629DOI Listing
April 2021

Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice.

Rice (N Y) 2021 Mar 20;14(1):32. Epub 2021 Mar 20.

Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, 518057, Guangdong, China.

Background: Carotenoid biosynthesis is essential for the generation of photosynthetic pigments, phytohormone production, and flower color development. The light harvesting like 3 (LIL3) protein, which belongs to the light-harvesting complex protein family in photosystems, interacts with geranylgeranyl reductase (GGR) and protochlorophyllide oxidoreductase (POR) both of which are known to regulate terpenoid and chlorophyll biosynthesis, respectively, in both rice and Arabidopsis.

Results: In our study, a CRISPR-Cas9 generated 4-bp deletion mutant oslil3 showed aberrant chloroplast development, growth defects, low fertility rates and reduced pigment contents. A comparative transcriptomic analysis of oslil3 suggested that differentially expressed genes (DEGs) involved in photosynthesis, cell wall modification, primary and secondary metabolism are differentially regulated in the mutant. Protein-protein interaction assays indicated that LIL3 interacts with phytoene synthase (PSY) and in addition the gene expression of PSY genes are regulated by LIL3. Subcellular localization of LIL3 and PSY suggested that both are thylakoid membrane anchored proteins in the chloroplast. We suggest that LIL3 directly interacts with PSY to regulate carotenoid biosynthesis.

Conclusion: This study reveals a new role of LIL3 in regulating pigment biosynthesis through interaction with the rate limiting enzyme PSY in carotenoid biosynthesis in rice presenting it as a putative target for genetic manipulation of pigment biosynthesis pathways in crop plants.
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http://dx.doi.org/10.1186/s12284-021-00474-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981378PMC
March 2021

SE-OnionNet: A Convolution Neural Network for Protein-Ligand Binding Affinity Prediction.

Front Genet 2020 19;11:607824. Epub 2021 Feb 19.

Trinity Earth Technology Co. Ltd, Beijing, China.

Deep learning methods, which can predict the binding affinity of a drug-target protein interaction, reduce the time and cost of drug discovery. In this study, we propose a novel deep convolutional neural network called SE-OnionNet, with two squeeze-and-excitation (SE) modules, to computationally predict the binding affinity of a protein-ligand complex. The OnionNet is used to extract a feature map from the three-dimensional structure of a protein-drug molecular complex. The SE module is added to the second and third convolutional layers to improve the non-linear expression of the network to improve model performance. Three different optimizers, stochastic gradient descent (SGD), Adam, and Adagrad, were also used to improve the performance of the model. A majority of protein-molecule complexes were used for training, and the comparative assessment of scoring functions (CASF-2016) was used as the benchmark. Experimental results show that our model performs better than OnionNet, Pafnucy, and AutoDock Vina. Finally, we chose the macrophage migration inhibitor factor (PDB ID: 6cbg) to test the stability and robustness of the model. We found that the prediction results were not affected by the docking position, and thus, our model is of acceptable robustness.
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http://dx.doi.org/10.3389/fgene.2020.607824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962986PMC
February 2021

Overexpression of Annexin A2 promotes proliferation by forming a Glypican 1/c-Myc positive feedback loop: prognostic significance in human glioma.

Cell Death Dis 2021 Mar 12;12(3):261. Epub 2021 Mar 12.

Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha, China.

In order to set up a reliable prediction system for the tumor grade and prognosis in glioma patients, we clarify the complicated crosstalk of Annexin A2 (ANXA2) with Glypican 1 (GPC1) and demonstrate whether combined indexes of ANXA2 and GPC1 could improve the prognostic evaluation for glioma patients. We found that ANXA2-induced glioma cell proliferation in a c-Myc-dependent manner. ANXA2 increased the expression of GPC1 via c-Myc and the upregulated GPC1 further promoted the c-Myc level, forming a positive feedback loop, which eventually led to enhanced proliferation of glioma cells. Both mRNA and protein levels of ANXA2 were upregulated in glioma tissues and coincided with the overexpression of GPC1. Besides, we utilized tissue microarrays (TMAs) and immunohistochemistry to demonstrate that glioma patients with both high expression of ANXA2 and GPC1 tended to have higher rate of tumor recurrence and shorter overall survival (OS). In conclusion, the overexpression of ANXA2 promotes proliferation of glioma cells by forming a GPC1/c-Myc positive feedback loop, and ANXA2 together with its downstream target GPC1 could be a potential "combination biomarker" for predicting prognosis of glioma patients.
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http://dx.doi.org/10.1038/s41419-021-03547-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954792PMC
March 2021

Automatic Classification of Hepatic Cystic Echinococcosis Using Ultrasound Images and Deep Learning.

J Ultrasound Med 2021 Mar 12. Epub 2021 Mar 12.

Ultrasonography Department, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.

Background: Hepatic cystic echinococcosis is the main form of hepatic echinococcosis, which is a life-threatening liver disease caused by parasites that requires a precise diagnosis and proper treatment.

Objective: This study focuses on the automatic classification system of five different subtypes of hepatic cystic echinococcosis based on ultrasound images and deep learning algorithms.

Methods: Three popular deep convolutional neural networks (VGG19, Inception-v3, and ResNet18) with and without pretrained weights were selected to test their performance on the classification task, and the experiments were followed by a 5-fold cross-validation process.

Results: A total of 1820 abdominal ultrasound images covering five subtypes of hepatic cystic echinococcosis from 967 patients were used in the study. The classification accuracy for the models with pretrained weights (fine-tuning) ranged from 88.2 to 90.6%. The best accuracy of 90.6% was obtained by VGG19. For comparison, the models without pretrained weights (from scratch) achieved a lower accuracy, ranging from 69.4 to 75.1%.

Conclusion: Deep convolutional neural networks with pretrained weights are capable of recognizing different subtypes of hepatic cystic echinococcosis from ultrasound images, which are expected to be applied in the computer-aided diagnosis systems in future work.
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http://dx.doi.org/10.1002/jum.15691DOI Listing
March 2021

Surgical Planning for Cleft Lip and/or Palate-Induced Moderate-to-Severe Midfacial Hypoplasia.

J Craniofac Surg 2021 Mar-Apr 01;32(2):492-495

Cleft Lip and Palate Department.

Background: Distraction osteogenesis and conventional bimaxillary orthognathic surgery have been performed for the treatment of midfacial hypoplasia for a long time. However, the effect of these 2 techniques on the maxilla, mandible, and whole-facial profile is significantly different. In this study, we aimed to measure the pre- to post-treatment changes in maxillary prominence, mandible size, and facial length and compare them between these 2 techniques to inform selection of the best technique.

Methods: This single-center, retrospective study included 35 patients with a cleft lip and/or palate-induced midfacial hypoplasia; 25 were treated using rigid external distraction osteogenesis and 10 using bimaxillary orthognathic surgery. Three-dimensional measures of changes in facial structure were obtained from reconstructed computed tomography images and used to compare the effects of the 2 techniques.

Results: Satisfactory appearance and occlusion were achieved in all patients. Three-dimensional reconstruction of the craniofacial skeleton revealed significant maxillary advancement (P < 0.001), mandibular (clockwise) rotation (P < 0.001), and increased facial length (P < 0.001) after rigid external distraction osteogenesis and obvious shortening of the mandibular body (P < 0.001) after bimaxillary orthognathic surgery.

Conclusion: Distraction osteogenesis can be selected as the first choice of treatment for cleft lip and/or palate-induced midfacial hypoplasia. A mandibular setback procedure can be performed as a second-stage surgery when severe temporomandibular joint complications develop with distraction osteogenesis. Bimaxillary orthognathic surgery results in an obvious shortening of the mandibular body, which is not a natural change in facial morphology.

Level Of Evidence: Therapeutic III.
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http://dx.doi.org/10.1097/SCS.0000000000006932DOI Listing
March 2021