Publications by authors named "Tao Ma"

706 Publications

Platelet-Rich Plasma versus Corticosteroid Injection for the Treatment of Lateral Epicondylitis: A Systematic Review of Systematic Reviews.

Int J Sports Phys Ther 2021 Jun 1;16(3):597-605. Epub 2021 Jun 1.

University of Nebraska at Omaha.

Background: Lateral epicondylitis (LE) is one of the most commonly reported musculoskeletal disorders in the upper extremity. The mechanism of LE is repetitive motion that causes a strain of the extensor tendons. This consequently causes pain and tendinosis at the tendinous attachment site on the lateral epicondyle. Most cases of LE are treated nonoperatively with a variety of interventions, such as injections.

Purpose: The aim of this systematic review (SR) is to synthesize the current evidence on the efficacy of platelet rich plasma (PRP) injections versus corticosteroid (CS) injections as treatment interventions for LE.

Study Design: Systematic Review.

Methods: Online databases were searched from database inception to February 24th, 2020 for relevant SR's evaluating PRP vs. CS injections as treatment methods for LE. Two independent researchers searched and screened for articles that were systematic reviews that directly compared PRP to CS injections for LE.

Results: A total of five SR's were included in this review that were published between 2016 and 2020. CS injections were more efficacious for short-term pain relief, and PRP injections were more efficacious for long-term pain relief and improved function.

Conclusion: PRP injections appear to be a more effective long-term treatment option than CS injections for those with LE who did not respond to conservative management.

Level Of Evidence: 1.
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http://dx.doi.org/10.26603/001c.24148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169032PMC
June 2021

A chromosome-level Camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis.

Nat Commun 2021 06 10;12(1):3531. Epub 2021 Jun 10.

Key Laboratory of Bio-resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.

Camptothecin and its derivatives are widely used for treating malignant tumors. Previous studies revealed only a limited number of candidate genes for camptothecin biosynthesis in Camptotheca acuminata, and it is still poorly understood how its biosynthesis of camptothecin has evolved. Here, we report a high-quality, chromosome-level C. acuminata genome assembly. We find that C. acuminata experiences an independent whole-genome duplication and numerous genes derive from it are related to camptothecin biosynthesis. Comparing with Catharanthus roseus, the loganic acid O-methyltransferase (LAMT) in C. acuminata fails to convert loganic acid into loganin. Instead, two secologanic acid synthases (SLASs) convert loganic acid to secologanic acid. The functional divergence of the LAMT gene and positive evolution of two SLAS genes, therefore, both contribute greatly to the camptothecin biosynthesis in C. acuminata. Our results emphasize the importance of high-quality genome assembly in identifying genetic changes in the evolutionary origin of a secondary metabolite.
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http://dx.doi.org/10.1038/s41467-021-23872-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192753PMC
June 2021

Facile extraction and characterization of cellulose nanocrystals from agricultural waste sugarcane straw.

J Sci Food Agric 2021 Jun 6. Epub 2021 Jun 6.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.

Background: Sugarcane straw is an available but largely ignored lignocellulosic biomass to obtain cellulose nanocrystals (CNCs) with highly crystalline, tunable surface chemistries and a wide-ranging adaptability. Herein, we utilized sugarcane straw to obtain pure cellulose via purification processes, followed by subsequent preparation of CNCs via sulfuric acid hydrolysis. The properties of the purified fibers and obtained CNCs were assessed by their composition, morphology, chemical structure, crystallinity and thermal stability.

Results: After purification process, alkali-treated fibers (ATFs) contained 886.33±1.25 g kg cellulose, and its morphological analysis revealed a smooth and slender fibrous structure. The CNCs obtained by treatment with 64 wt% sulfuric acid at 45°C for 60 min were isolated in a yield of 21.8%, with a diameter and length of 6 ~ 10 nm and 160 ~ 200 nm, respectively. Moreover, crystallinity index of these CNCs reached 62.66%, and thermal stability underwent a two-step degradation. Short-term ultrasonication after hydrolysis was employed to enhance isolation of the CNC particles and improve the anionic charge with higher value -38.00 mV.

Conclusion: Overall, isolation and characterization results indicated the potential for CNCs preparation using sugarcane straw, in addition to offering a fundamental understanding of this material and indicating potential applications. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jsfa.11360DOI Listing
June 2021

Associations Between Single-Child Status and Metabolic Syndrome in Children and Adolescents in China.

Front Pediatr 2021 20;9:661164. Epub 2021 May 20.

Menzies Health Institute Queensland, Griffith University, Brisbane, QLD, Australia.

To evaluate the associations between single-child status and metabolic syndrome (MS) and to identify the highest risk group of MS among single children. Differences in participants' characteristics by sex were examined by Student's -test for continuous variables and Pearson's chi-squared test for categorical variables. Multivariate logistic regression analysis was performed to estimate the odds ratios () and 95% confidence intervals () for MS and its components according to the single-child status. Radar maps were used to compare the composition of different components in MS. In total, 11,784 (5,880 boys) children and adolescents were included in this study, with a mean age of (11.3 ± 3.1) years. MS was observed in 7.1% of participants, with a higher prevalence in boys (8.2%) than girls (5.9%) ( < 0.05). The prevalence of MS, elevated blood pressure and abdominal obesity in single children were higher than that in children with siblings, particularly in boys ( < 0.001). Elevated risk of abdominal obesity was observed in single children [boys (1.56, 95% CI: 1.31, 1.85), girls (1.40, 95% CI: 1.19, 1.63)], however, increased ORs of elevated blood pressure and metabolic syndrome were observed in single-child boys only (1.19, 95% CI: 1.01, 1.40 and 1.76, 95% CI: 1.34, 2.31, respectively). Results showed that a statistically significant association between single child status and MS was mainly observed in urban boys (2.04, 95% CI: 1.33, 3.12) and rural boys (1.50, 95% CI: 1.05, 2.15), but not in girls. Among all the combinations of MS, two combinations were significantly associated with the single-child status, including the combination of elevated blood pressure, abdominal and low HDL-C (1.45, 1.04, 2.04) and the combination of elevated blood pressure, abdominal obesity, low HDL-C and hypertriglyceridemia (2.04, 1.40, 3.06) ( < 0.05). The present study found that single children and adolescents had a higher risk of MS, elevated blood pressure and abdominal obesity. The associations were stronger in urban boys. Further attention should be directed to the prevention and control strategies targeting the high-risk population of MS.
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http://dx.doi.org/10.3389/fped.2021.661164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173115PMC
May 2021

Analysis of Serum miRNAs in Alzheimer's Disease.

Am J Alzheimers Dis Other Demen 2021 Jan-Dec;36:15333175211021712

Department of Neurology, The Affiliated WuXi No.2 People's Hospital of Nanjing Medical University, Wuxi, China.

This paper was aimed to analyze the microRNA (miRNA) signatures in Alzheimer disease (AD) and find the significant expressions of miRNAs, their target genes, the functional enrichment analysis of the confirmed genes, and potential drug treatment. The miRNA expression information of the gene expression profile data was downloaded from the Gene Expression Omnibus database. The total data sample size is 1309, including 1021 AD samples and 288 normal samples. A total of 21 differentially expressed miRNAs were obtained, of which 16 (hsa-miR-6761-3p, hsa-miR-6747-3p, hsa-miR-6875-3p, hsa-miR-6754-3p, hsa-miR-6736-3p, hsa-miR-6762-3p, hsa-miR-6787-3p, hsa-miR-208a-5p, hsa-miR-6740-3p, hsa-miR-6778-3p, hsa-miR-595, hsa-miR-6753-3p, hsa-miR-4747-3p, hsa-miR-3646, hsa-miR-6716-3p and hsa-miR-4435) were up-regulated and 5 (hsa-miR-125a-3p, hsa-miR-22-3p, hsa-miR-24-3p, hsa-miR-6131 and hsa-miR-125b-1-3p) were down-regulated in AD. A total of 6 miRNAs (hsa-miR-595, hsa-miR-3646, hsa-miR-4435 hsa-miR-125a-3p, hsa-miR-22-3p and hsa-miR-24-3p) and 78 miRNA-disease-related gene sub-networks were predicted, and 116 ceRNA regulatory relationship pairs, and the ceRNA regulatory network were obtained. The results of enrichment analysis suggested that the main target pathways of several miRNAs differentially expressed in AD were mitogen-activated protein kinase signal pathway. According to the prediction results of Drug-Gene Interaction database 2.0, we obtained 53 pairs of drug-gene interaction, including 7 genes (PTGS2, EGFR, CALM1, PDE4D, FGFR2, HMGCR, cdk6) and 53 drugs. We hope our results are helpful to find a viable way to prevent, delay the onset, diagnose, and treat AD.
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http://dx.doi.org/10.1177/15333175211021712DOI Listing
June 2021

ER stress and its PERK branch enhance TCR-induced activation in regulatory T cells.

Biochem Biophys Res Commun 2021 Jul 28;563:8-14. Epub 2021 May 28.

Department of Microbiology and Immunology, Trauma Research Center, Fourth Medical Center of the Chinese PLA General Hospital, Beijing, PR China. Electronic address:

Although accumulating evidence indicates participation of endoplasmic reticulum (ER) stress pathway or the unfolded protein response (UPR) in immunity, there still exists little information linking ER stress to regulatory T cells (Tregs). To evaluate the potential contribution of the UPR, we tested the effects of thapsigargin (TG), an ER stress inducer, on the function of Tregs. Here we reported that TG stimulation induced the up-regulation of the endoplasmic reticulum (ER)-stress chaperon Glucose-Regulated Protein 78 (GRP78), which is a master regulator of the UPR, the phosphorylation of eukaryotic initiation factor 2 alpha (elF2α) and the induction of activating transcription factor 4 (ATF4), which are both protein kinase R (PKR)-like ER kinase (PERK) downstream targets in Tregs. Simultaneously, we demonstrated that, under ER stress conditions, Tregs presented enhanced functional activity upon TCR stimulation, as illustrated with forkhead box transcription factor (Foxp3) expression, interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) production and suppressive functional analysis. Notably, pretreatment with GSK2656157, a potent and selective PERK inhibitor, markedly diminished the TG-induced hyperresponsiveness of Tregs upon T cell antigen receptor (TCR) stimulation. Therefore, our findings illustrated the inter-connection and coordination of the evolutionarily conserved ER stress response and TCR signaling in Tregs and uncover a critical new role of the PERK branch of UPR in the regulation of Tregs function.
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http://dx.doi.org/10.1016/j.bbrc.2021.05.061DOI Listing
July 2021

A two-photon "turn-on" fluorescent probe for both exogenous and endogenous selenocysteine detection and imaging in living cells and zebrafish.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 20;260:119983. Epub 2021 May 20.

National Engineering Laboratory for Deep Process of Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, PR China. Electronic address:

Selenocysteine (Sec) is recognized as the 21st amino acid employing as an essential building block for selenoproteins (SePs), which plays a significant role in various physiological processes. Therefore, there is an urgent need to reasonably develop some reliable and rapid methods for Sec detection in biological systems. In this work, we reported a new two-photon (TP) fluorescent probe BNT-Sec for Sec detection and imaging in living cells and zebrafish with two part: (1) a D-π-A-structured naphthalene derivative as a TP fluorophore; (2) a well-know Sec responsive site with strong intromolecular charge transfer effect (ICT) to selectively detect endogenous and exogenous. In the presence of Sec, probe BNT-Sec can initiate a Se-dependent specific aromatic nucleophilic substitution reaction, which exhibited BNT-Sec had a large fluorescence intensity enhancement with ~18.9-fold at 510 nm, a high sensitivity low LOD value' 10.6 nM, good light stability, strong specificity, pH stability and low cytotoxicity. In addition, BNT-Sec can be conveniently used to detect Sec in living cells and zebrafish for TP imaging due to the great TP measurement properties of fluorophore, exhibiting it has the potential to reveal the role of selenocysteine in physiological and pathological processes in further biological applications.
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http://dx.doi.org/10.1016/j.saa.2021.119983DOI Listing
November 2021

Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning.

Nat Commun 2021 05 28;12(1):3238. Epub 2021 May 28.

Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, Qingdao, China.

The design of CRISPR gRNAs requires accurate on-target efficiency predictions, which demand high-quality gRNA activity data and efficient modeling. To advance, we here report on the generation of on-target gRNA activity data for 10,592 SpCas9 gRNAs. Integrating these with complementary published data, we train a deep learning model, CRISPRon, on 23,902 gRNAs. Compared to existing tools, CRISPRon exhibits significantly higher prediction performances on four test datasets not overlapping with training data used for the development of these tools. Furthermore, we present an interactive gRNA design webserver based on the CRISPRon standalone software, both available via https://rth.dk/resources/crispr/ . CRISPRon advances CRISPR applications by providing more accurate gRNA efficiency predictions than the existing tools.
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http://dx.doi.org/10.1038/s41467-021-23576-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163799PMC
May 2021

Lipolysis drives expression of the constitutively active receptor GPR3 to induce adipose thermogenesis.

Cell 2021 May 22. Epub 2021 May 22.

Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.

Thermogenic adipocytes possess a therapeutically appealing, energy-expending capacity, which is canonically cold-induced by ligand-dependent activation of β-adrenergic G protein-coupled receptors (GPCRs). Here, we uncover an alternate paradigm of GPCR-mediated adipose thermogenesis through the constitutively active receptor, GPR3. We show that the N terminus of GPR3 confers intrinsic signaling activity, resulting in continuous Gs-coupling and cAMP production without an exogenous ligand. Thus, transcriptional induction of Gpr3 represents the regulatory parallel to ligand-binding of conventional GPCRs. Consequently, increasing Gpr3 expression in thermogenic adipocytes is alone sufficient to drive energy expenditure and counteract metabolic disease in mice. Gpr3 transcription is cold-stimulated by a lipolytic signal, and dietary fat potentiates GPR3-dependent thermogenesis to amplify the response to caloric excess. Moreover, we find GPR3 to be an essential, adrenergic-independent regulator of human brown adipocytes. Taken together, our findings reveal a noncanonical mechanism of GPCR control and thermogenic activation through the lipolysis-induced expression of constitutively active GPR3.
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http://dx.doi.org/10.1016/j.cell.2021.04.037DOI Listing
May 2021

Quercetin Alleviates Neuropathic Pain in the Rat CCI Model by Mediating AMPK/MAPK Pathway.

J Pain Res 2021 19;14:1289-1301. Epub 2021 May 19.

Department of Anesthesiology and Pharmacology, Xuzhou Medical University, Xuzhou, 221000, People's Republic of China.

Context: Quercetin (que) is one abundant flavonol with a variety of biological activities. Previous studies have shown quercetin can reduce neuropathic pain in rats with chronic constriction injury (CCI).

Objective: To evaluate the effects of quercetin on neuropathic pain in CCI model and explore its underlying mechanism in vivo.

Materials And Methods: CCI model was established by ligating the sciatic nerve of right leg on the SD rats. They were divided into ten groups: sham group, CCI model, sham+ que, CCI+ que group (30, 60, 120 mg/kg), CCI+ AICAR, CCI+ que+ compound C, CCI+etoricoxib, and the control group. They were administered for 28 days, and were performed the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) during the experiment. At the end of the experiment, sciatic nerves and spinal cord segments of rats were collected, ELISA detected the expression of inflammatory factors, detected the microglia and astrocytes with fluorescence, and Western blot detected AMPK/MAPK pathway.

Results: Que could increase the MWT of CCI rats, improve the TWL of plantar, and reduce the inflammatory cells at the ligation site of the sciatic nerve. Also, que could reduce the levels of TNF-α, IL-6, and IL-1β. Western blotting results showed that p-38 MAPK, p-ERK, and p-JNK were activated in the spinal dorsal horn of CCI model group. After treatment with que and AMPK agonists, the phosphorylation levels of related proteins were inhibited. In addition, the analgesic effect of que was abolished when the AMPK inhibitor was added.

Discussion And Conclusion: Quercetin alleviated the inflammatory response of sciatic nerve and spinal dorsal horn in rats induced by CCI. Quercetin alleviates neuralgia in CCI rats by activating AMPK pathway and inhibiting MAPK pathway and its downstream targets, p-38, p-ERK, and p-JNK.
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http://dx.doi.org/10.2147/JPR.S298727DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141401PMC
May 2021

[China's Reuse Water Development and Utilization Potential Based on the RDA-REM Model].

Huan Jing Ke Xue 2021 Jun;42(6):2758-2768

Nanjing Hydraulic Research Institute, Nanjing 210029, China.

To promote the efficient utilization of China's reuse water resources and optimize the allocation of water resources, an analysis of factors influencing the development and utilization of reuse water resources was conducted. The uniqueness and competitiveness of reuse water resources were analyzed, and the driving and constraint mechanisms were revealed. A potential indicator system for the bilateral coordination of the supply and demand of reuse water was also established. Based on redundancy analysis (RDA), key indicators for the prediction of reuse water development and utilization potential were screened. On this basis, a national-scale reuse water development and utilization potential prediction model was constructed (the random effects model, REM). Given some uncertainty in the parameters of the REM model, the confidence interval ranges of the parameters at the 10%-90% quartile levels were identified. The results show that four indicators (ecological water consumption, density of water supply pipelines in built-up areas, fixed asset investment in the construction of reuse water treatment facilities, and total wastewater treatment) are closely related to the development and utilization of reuse water and, hence, are key indicators. The REM for the potential prediction has a high fitting accuracy, which can effectively reflect the fluctuations in the observed values with a maximum fitting error of -8.5%. China's reuse water development and utilization will continue to maintain rapid growth long into the future, reaching 12.9 billion m by 2025. This will help optimize national urban water supply structures and improve the reuse rate of regional water resources.
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http://dx.doi.org/10.13227/j.hjkx.202009068DOI Listing
June 2021

Genome-wide (ChIP-seq) identification of target genes regulated by WRKY33 during submergence stress in Arabidopsis.

BMC Genom Data 2021 05 24;22(1):16. Epub 2021 May 24.

Key Laboratory for Bio-resources and Eco-environment & State Key Lab of Hydraulics & Mountain River Engineering, College of Life Science, Sichuan University, Chengdu, 610065, China.

Background: Hypoxia induced by flooding causes significant losses to crop production almost every year. However, the molecular network of submergence signaling pathway is still poorly understood. According to previous studies, transgenic plants overexpressing the WRKY33 gene showed enhanced resistance to submergence stress. Thus, this transcription factor may regulate a series of target genes in response to submergence. Here, to determine putative downstream targets of WRKY33 at a genome-wide scale in Arabidopsis thaliana, we performed the chromatin immunoprecipitation sequencing (ChIP-seq) using 35S:FLAG-WRKY33 overexpression transgenic lines (WRKY33-OE) after 24 h of submergence treatment.

Results: Using ChIP-seq data, we identified a total of 104 WRKY33-binding genes under submergence stress (WRKY33BGSs). Most WRKY33BGSs are involved in the oxidation-reduction process, programmed cell death in response to reactive oxygen species, lipid biosynthesis process, and other processes related to stress responses. Moreover, the major motif identified in the WRKY33BGSs promoters is a new cis-element, TCTCTC (named here as "TC box"). This cis-element differs from the previously known W box for WRKY33. Further qPCR experiments verified that genes carrying this motif in their promoters could be regulated by WRKY33 upon submergence treatment.

Conclusions: Our study has identified a new putative binding motif of WRKY33 and recovered numerous previously unknown target genes of WRKY33 during submergence stress. The WRKY33 gene positively participates in flooding response probably by transcriptional regulation of the downstream submergence-related target genes via a "TC box".
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http://dx.doi.org/10.1186/s12863-021-00972-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142642PMC
May 2021

Highly efficient blackberry-like trimetallic PdAuCu nanoparticles with optimized Pd content for ethanol electrooxidation.

Nanoscale 2021 Jun 21;13(22):9960-9970. Epub 2021 May 21.

The State Key Laboratory of Refractories and Metallurgy, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, P. R. China.

The rational design of highly efficient catalysts for ethanol electrooxidation is extremely challenging for developing direct ethanol fuel cells (DEFCs). Herein, a facile one-pot method has been developed to prepare blackberry-like PdAuCu nanoparticles (NPs) with tunable composition and surface structures. Among PdAuCu NPs with different Pd contents (1.6-22 mass%), PdAuCu NPs-0.5 (contained Pd at 2.5 mass%) delivered one of the highest catalytic activities of Pd-based catalysts towards ethanol electrooxidation, exhibiting a mass activity of 23.0 A mg. Kinetic analysis, electrochemical impedance spectroscopy and CO stripping test results suggested that the excellent electrocatalytic activity may originate from the optimized balance between Pd content and surface structure of PdAuCu NPs-0.5. The optimization of the balance between composition and surface structure would contribute to the further design of multimetallic nanoparticles for fuel cells and other applications.
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http://dx.doi.org/10.1039/d1nr00841bDOI Listing
June 2021

[Short-term effectiveness of femoral neck system in the treatment of femoral neck fracture].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 May;35(5):539-543

Orthopedics Center, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan Ningxia, 750001, P.R.China.

Objective: To investigate the short-term effectiveness of femoral neck system (FNS) in the treatment of femoral neck fracture.

Methods: The clinical data of 34 patients with femoral neck fracture admitted between January 2019 and April 2020 who met the selection criteria were retrospectively analyzed and divided into group A (19 patients were treated with conventional cannulated screw internal fixation) and group B (15 patients were treated with FNS internal fixation) according to the different methods of internal fixation. There was no significant difference between the two groups in general data such as gender, age, affected side, cause of injury, fracture type, and time from injury to operation ( >0.05). The operation time, intraoperative blood loss, fluoroscopy frequency, and fracture healing time were recorded and compared between the two groups. X-ray film and CT examinations were performed postoperatively to evaluate fracture reduction and internal fixation, and the shortening of the femoral neck on the affected side was measured compared with that on the healthy side. The Harris score was used to evaluate hip function.

Results: There was 1 unsatisfied reduction case in groups A and B respectively, the rest of the patients in both groups were obtained satisfied reduction. There was no significant difference in the quality of reduction between the two groups ( =-0.195, =0.854). There was no significant difference in operation time between the two groups ( =0.649, =0.064). The intraoperative blood loss in group A was significantly less than that in group B, and the fluoroscopy frequency was significantly more than that in group B, with significant differences ( <0.05). Except for 1 case in group A with screw out at 3 months after operation and no obvious callus formation, all fractures in the two groups reached clinical healing, and there was no significant difference in fracture healing time between the two groups ( =-0.127, =0.899). There was no necrosis of femoral head in the two groups. At last follow-up, there were 4 cases of femoral neck shortening in group A and 2 cases in group B. The hip function of both groups recovered well, and there was no significant difference in Harris score at last follow-up ( =0.956, =0.346).

Conclusion: The treatment of femoral neck fracture using FNS has less trauma. Compared with cannulated screw internal fixation, it can reduce the intraoperative fluoroscopy frequency and obtain satisfactory short-term effectiveness.
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http://dx.doi.org/10.7507/1002-1892.202012097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175206PMC
May 2021

Case report of a rare giant bone island in a vertebral body combined with hemangioma.

J Int Med Res 2021 May;49(5):3000605211010699

Department of Orthopaedics, The First People's Hospital of Longquanyi District, Chengdu, Sichuan, China.

This case report describes a rare giant bone island combined with hemangioma diagnosed in a patient with osteolytic vertebral metastases. The bone island's greatest diameter was 3.15 cm, and bone islands of this size are rare in the literature. This article aims to provide clinicians with information about the diagnosis and relevant literature of bone islands.
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http://dx.doi.org/10.1177/03000605211010699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127800PMC
May 2021

Cold-induction of afadin in brown fat supports its thermogenic capacity.

Sci Rep 2021 May 7;11(1):9794. Epub 2021 May 7.

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

The profound energy-expending nature of brown adipose tissue (BAT) thermogenesis makes it an attractive target tissue to combat obesity-associated metabolic disorders. While cold exposure is the strongest inducer of BAT activity, the temporal mechanisms tuning BAT adaptation during this activation process are incompletely understood. Here we show that the scaffold protein Afadin is dynamically regulated by cold in BAT, and participates in cold acclimation. Cold exposure acutely increases Afadin protein levels and its phosphorylation in BAT. Knockdown of Afadin in brown pre-adipocytes does not alter adipogenesis but restricts β-adrenegic induction of thermogenic genes expression and HSL phosphorylation in mature brown adipocytes. Consistent with a defect in thermogenesis, an impaired cold tolerance was observed in fat-specific Afadin knockout mice. However, while Afadin depletion led to reduced Ucp1 mRNA induction by cold, stimulation of Ucp1 protein was conserved. Transcriptomic analysis revealed that fat-specific ablation of Afadin led to decreased functional enrichment of gene sets controlling essential metabolic functions at thermoneutrality in BAT, whereas it led to an altered reprogramming in response to cold, with enhanced enrichment of different pathways related to metabolism and remodeling. Collectively, we demonstrate a role for Afadin in supporting the adrenergic response in brown adipocytes and BAT function.
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http://dx.doi.org/10.1038/s41598-021-89207-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105362PMC
May 2021

Circular RNA hsa_circ_0013958 Functions as an Oncogenic Gene Through Modulating miR-532-3p/WEE1 Axis in Hepatocellular Carcinoma.

Front Oncol 2021 15;11:585172. Epub 2021 Apr 15.

Department of Emergency Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: circ0013958 was identified as a biomarker, which can be used for the diagnosis and screening of lung cancer. However, the role of circ0013958 in hepatocellular carcinoma (HCC) remains unclear.

Methods: In our study, quantitative real-time polymerase chain reaction was performed to determine the levels of circ0013958 in HCC tissues and cell lines. EdU, CCK-8, transwell, flow cytometry and tumorigenesis assays were applied to assess the functions of circ0013958 in HCC and . Western blot assay was to detect the expression of WEE1. Luciferase reporter assay, bioinformatics analysis and rescue experiments were used to examine the interaction among circ0013958, miR-532-3p and WEE1.

Results: It revealed that circ0013958 was significantly up-regulated in HCC, which was positively correlated with poor prognosis of HCC patients. Circ0013958 promoted HCC cell proliferation and invasion, inhibited cell apoptosis , and promoted tumorigenesis . Circ0013958 acted as a miR-532-3p sponge to regulate WEE1 expression, thus promoting the progression of HCC.

Conclusions: Circ0013958 promotes HCC progression through miR-532-3p/WEE1 axis. Circ0013958 may serve as a potential diagnostic biomarker and therapeutic target of HCC.
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http://dx.doi.org/10.3389/fonc.2021.585172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082454PMC
April 2021

One-Dimensional Superlattice Heterostructure Library.

J Am Chem Soc 2021 May 30;143(18):7013-7020. Epub 2021 Apr 30.

Division of Nanomaterials and Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Institute of Energy, Hefei Comprehensive National Science Center, CAS Center for Excellence in Nanoscience, Department of Chemistry, Institute of Biomimetic Materials and Chemistry, Anhui Engineering Laboratory of Biomimetic Materials, University of Science and Technology of China, Hefei, Anhui 230026, China.

Axially, epitaxially organizing nano-objects of distinct compositions and structures into superlattice nanowires enables full utilization of sunlight, readily engineered band structures, and tunable geometric parameters to fit carrier transport, thus holding great promise for optoelectronics and solar-to-fuel conversion. To maximize their efficiency, the general and high-precision synthesis of colloidal axial superlattice nanowires (ASLNWs) with programmable compositions and structures is the prerequisite; however, it remains challenging. Here, we report an axial encoding methodology toward the ASLNW library with precise control over their compositions, dimensions, crystal phases, interfaces, and periodicity. Using a predesigned, editable nanoparticle framework that offers the synthetic selectivity, we are able to chemically decouple adjacent sub-objects in ASLNWs and thus craft them in a controlled approach, yielding a library of distinct ASLNWs. We integrate therein plasmonic, metallic, or near-infrared-active chalcogenides, which hold great potential in solar energy conversion. Such synthetic capability enables a performance boost in target applications, as we report order-of-magnitude enhanced photocatalytic hydrogen production rates using optimized ASLNWs compared to corresponding solo objects. Furthermore, it is expected that such unique superlattice nanowires could bring out new phenomena.
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http://dx.doi.org/10.1021/jacs.1c01514DOI Listing
May 2021

Clinical features and outcomes after endovascular therapy for penetrating aortic ulcer and intramural hematoma.

Vascular 2021 Apr 28:17085381211012573. Epub 2021 Apr 28.

Department of Vascular Surgery, Institute of Vascular Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Objectives: To identify the differences between clinical features and outcomes after endovascular therapy for penetrating aortic ulcer (PAU) and intramural hematoma (IMH).

Methods: From January 2009 to March 2020, patients who underwent endovascular therapy for PAU and IMH were enrolled. Information on patient demographics, presentation, PAU and IMH morphology, laboratory examination, and clinical follow-up information was collected and analyzed. Univariate analysis was performed to identify the differences between IMH and PAU, and Kaplan-Meier was used to calculate the cumulative survival rate and freedom from reintervention.

Results: A total of 114 patients were enrolled; 80 (70.2%) of them were diagnosed with PAU. Compared with PAU, patients with IMH were younger ( = 0.006), more likely to be admitted emergently ( = 0.001), had longer hospital stay ( = 0.028), and had higher levels of C-reactive protein ( = 0.030). Meanwhile, patients with IMH were more likely to be associated with hypertension ( = 0.020) and pleural effusion ( < 0.001) and less likely to have a history of acute coronary syndrome ( = 0.019) and prior cardiovascular intervention ( = 0.017). The five-year freedom from reintervention and cumulative survival rate were 94.2% (95% confidential interval, 88.9%-99.9%) and 87.8% (95% confidential interval, 79.5%-96.9%) in PAU patients and 89.6% (95% confidential interval, 75.8%-99.9%) and 85.1% (95% confidential interval, 68.0%-99.9%) in IMH patients, respectively. There was no significant difference in freedom from reintervention ( = 0.795) or cumulative survival rate ( = 0.817).

Conclusions: IMH appeared to occur in younger patients with hypertension and usually had an acute onset, while PAU was more likely to be found incidentally in older patients with atherosclerosis. Endovascular therapy was effective in both IMH and PAU patients with encouraging outcomes.
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http://dx.doi.org/10.1177/17085381211012573DOI Listing
April 2021

Continuous-flow electrosynthesis of selenium-substituted iminoisobenzofuran via oxidative cyclization of olefinic amides and diselenides.

Org Biomol Chem 2021 Apr 24;19(14):3207-3212. Epub 2021 Mar 24.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhu Rd S., Nanjing 211816, China.

A green and efficient approach for the synthesis of selenium-substituted iminoisobenzofuran using 2-vinylbenzamides and diselenides in a continuous electrochemical microreactor has been developed. This strategy enabled the preparation of a series of iminoisobenzofuran derivatives in moderate to good yields under metal-free and oxidant-free conditions. The application of the electrochemical flow system successfully overcomes the difficulty of process control in traditional electrochemistry and achieves efficient transformation of electricity. Moreover, the continuous-flow system combined with electrosynthesis overcomes the difficulty in realizing a scale-up reaction in conventional batch-type electrolysis.
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http://dx.doi.org/10.1039/d1ob00236hDOI Listing
April 2021

The proportion and effect of corticosteroid therapy in patients with COVID-19 infection: A systematic review and meta-analysis.

PLoS One 2021 21;16(4):e0249481. Epub 2021 Apr 21.

Department of Orthopedics, Honghui Hospital, Xi'anJiaotong University, Xi'an, PR China.

Objectives: Coronavirus disease 2019 (COVID-19) remains a global challenge. Corticosteroids constitute a group of anti-inflammatory and immunosuppressive drugs that are widely used in the treatment of COVID-19. Comprehensive reviews investigating the comparative proportion and efficacy of corticosteroid use are scarce. Therefore, we conducted a systematic review and meta-analysis of clinical trials to evaluate the proportion and efficacy of corticosteroid use for the treatment of COVID-19.

Methods: We conducted a comprehensive literature review and meta-analysis of research articles, including observational studies and clinical trials, by searching the PubMed, EMBASE, Cochrane Controlled Trials Registry, and China Academic Journal Network Publishing databases. Patients treated between December 1, 2019, and January 1, 2021, were included. The outcome measures were the proportion of patients treated with corticosteroids, viral clearance and mortality. The effect size with the associated 95% confidence interval is reported as the weighted mean difference for continuous outcomes and the odds ratio for dichotomous outcomes.

Results: Fifty-two trials involving 15710 patients were included. The meta-analysis demonstrated that the proportion of COVID-19 patients who received corticosteroids was significantly lower than that of patients who did not receive corticosteroids (35.19% vs. 64.49%). In addition, our meta-analysis demonstrated no significant difference in the proportions of severe and nonsevere cases treated with corticosteroids (27.91% vs. 20.91%). We also performed subgroup analyses stratified by whether patients stayed in the intensive care unit (ICU) and found that the proportion of patients who received corticosteroids was significantly higher among those who stayed in the ICU than among those who did not. The results of our meta-analysis indicate that corticosteroid treatment significantly delayed the viral clearance time. Finally, our meta-analysis demonstrated no significant difference in the use of corticosteroids for COVID-19 between patients who died and those who survived. This result indicates that mortality is not correlated with corticosteroid therapy.

Conclusion: The proportion of COVID-19 patients who received corticosteroids was significantly lower than that of patients who did not receive corticosteroids. Corticosteroid use in subjects with severe acute respiratory syndrome coronavirus 2 infections delayed viral clearance and did not convincingly improve survival; therefore, corticosteroids should be used with extreme caution in the treatment of COVID-19.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249481PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059814PMC
May 2021

Large-Area Crystalline Zeolitic Imidazolate Framework Thin Films.

Angew Chem Int Ed Engl 2021 Jun 14;60(25):14124-14130. Epub 2021 May 14.

Division of Nanomaterials & Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Institute of Energy, Hefei Comprehensive National Science Center, CAS Center for Excellence in Nanoscience, Institute of Biomimetic Materials & Chemistry, Anhui Engineering Laboratory of Biomimetic Materials, Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China.

We report that continuous MOF films with highly controlled thickness (from 44 to 5100 nm) can be deposited over length scales greater than 80 centimeters by a facile, fast, and cost-effective spray-coating method. Such success relies on our discovery of unprecedented perfectly dispersed colloidal solutions consisting of amorphous MOF nanoparticles, which we adopted as precursors that readily converted to the crystalline films upon low-temperature in situ heating. The colloidal solutions allow for the fabrication of compact and uniform MOF films on a great deal of substrates such as fluorine-doped tin oxide, glass, SiO , Al O , Si, Cu, and even flexible polycarbonate, widening their technological applications where substrates are essential. Despite the present work focuses on the fabrication of uniform cobalt-(2-methylimidazole) and zinc-(2-methylimidazole) films, our findings mark a great possibility in producing other high-quality MOF thin films on a large scale.
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http://dx.doi.org/10.1002/anie.202104366DOI Listing
June 2021

Removal of inorganic arsenic from aqueous solution by Fe-modified ceramsite: batch studies and remediation trials.

Water Sci Technol 2021 Apr;83(7):1522-1534

School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao, Shandong Province 266033, China E-mail:

During sediment remediation, adsorbent addition is an effective technology for the removal of contaminants but the cost is often high. In this study, a low-cost adsorbent, ceramsite, made from contaminated riverbed sediment was synthesized. The Fe-modified ceramsite (FMC) was used as adsorbent to remove arsenate from aqueous solutions and reduce the inorganic arsenic release from contaminated sediments. Kinetic studies showed that chemisorption mainly governed the adsorption process while batch studies yielded the theoretical adsorption capacity for arsenate of 10.63 mg/g at pH = 7 condition. Co-existing anions and pH have no significant impact on the adsorption process. In the regeneration studies, 91, 86, and 80% of the adsorption capacity were recovered in 3 cycles. In-situ remediation trials revealed that the addition of the adsorbent to sediment surface significantly reduced the release of inorganic arsenic into aqueous system, with a reduction efficiency of 86%. Furthermore, the species of the arsenic in the surface layer was significantly inactivated from an active state to a stable state. These findings highlight the application of the FMC as a facile and cost-effective adsorbent for containment of arsenic in solutions and sediments, demonstrating that they are highly applicable for practical cases.
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http://dx.doi.org/10.2166/wst.2021.076DOI Listing
April 2021

Prognostic value of serum pentraxin 3 for intracerebral hemorrhage mortality.

J Integr Neurosci 2021 Mar;20(1):137-142

Department of Neurosurgery, The First Affiliated Hospital of Xi'an Medical University, 710077 Xi'an, P. R. China.

Pentraxin 3 is considered an important inflammatory marker is known to increase in patients with ischemic stroke, but the relationship between pentraxin 3 and intracerebral hemorrhage mortality is unclear. The purpose of this study is to investigate the level of pentraxin 3 in serum and its impact on prognosis in 307 patients with intracerebral hemorrhage. During the 5-year follow-up, the mortality rate of patients with intracerebral hemorrhage was 22.5%. The serum pentraxin 3 level of the brain-dead patients was higher than that of the control group ( < 0.05). Logistic regression analysis indicated a high correlation between the pentraxin 3 level and the mortality rate 95% (hazard ratio: 3.671; confidence interval: 1.558-4.297). The receiver operating characteristic curve showed that pentraxin 3 (Area Under Curve = 0.801) had a higher diagnostic value than C-reactive protein (Area Under Curve = 0.701). The pentraxin 3 level increased significantly after intracerebral hemorrhage and has an important predictive value for a prognosis for intracerebral hemorrhage mortality.
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http://dx.doi.org/10.31083/j.jin.2021.01.228DOI Listing
March 2021

Metal-Free -Selective C-H Borylation of 2-Phenylthiopyridines Using BBr.

J Org Chem 2021 Apr 8;86(8):5933-5942. Epub 2021 Apr 8.

Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education; School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.

A novel route for -selective C-H borylation of 2-phenylthiopyridines using BBr as the boron source under metal-free conditions has been reported. The reaction exhibited site exclusivity, and the synthesized aryl boronates were freely converted to various useful intermediates. Thus, this facile method would be beneficial to synthesize structurally diversified phenylthioethers derivatives and other materials containing boron-nitrogen coordination.
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http://dx.doi.org/10.1021/acs.joc.1c00520DOI Listing
April 2021

Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.

Oncogene 2021 Apr 6;40(17):3136-3151. Epub 2021 Apr 6.

Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Therapeutic strategies to treat pancreatic ductal adenocarcinoma (PDAC) remain unsatisfying and limited. Therefore, it is imperative to fully determine the mechanisms underlying PDAC progression. In the present study, we report a novel role of regulator of calcineurin 1, isoform 4 (RCAN1.4) in regulating PDAC progression. We demonstrated that RCAN1.4 expression was decreased significantly in PDAC tissues compared with that in para-cancerous tissues, and correlated with poor prognosis of patients with pancreatic cancer. In vitro, stable high expression of RCAN1.4 could suppress the metastasis and proliferation and angiogenesis of pancreatic tumor cells. In addition, interferon alpha inducible protein 27 (IFI27) was identified as having a functional role in RCAN1.4-mediated PDAC migration and invasion, while VEGFA play a vital role in RCAN1.4-mediated PDAC angiogenesis. Analysis of mice with subcutaneously/orthotopic implanted xenograft tumors and liver metastasis model confirmed that RCAN1.4 could modulate the growth, metastasis, and angiogenesis of tumors via IFI27/VEGFA in vivo. In conclusion, our results suggested that RCAN1.4 suppresses the growth, metastasis, and angiogenesis of PDAC, functioning partly via IFI27 and VEGFA. Importantly, our results provided possible diagnostic criteria and therapeutic targets for PDAC.
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http://dx.doi.org/10.1038/s41388-021-01763-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084734PMC
April 2021

The Essential Involvement of the Omentum in the Peritoneal Defensive Mechanisms During Intra-Abdominal Sepsis.

Front Immunol 2021 18;12:631609. Epub 2021 Mar 18.

Department of Microbiology and Immunology, Trauma Research Center, Fourth Medical Center of the Chinese PLA General Hospital, Beijing, China.

Although the abilities of the omentum to alleviate inflammation and prevent infection have been revealed over the past decades, the underlying mechanisms remain largely unelucidated. Here, we demonstrated that the mortality of mice exposed to cecal ligation and puncture (CLP) and omentectomy was remarkably increased compared to those treated with CLP alone. Moreover, the efficacy of the omentum was associated with an impairment in intraperitoneal bacterial clearance together with an increase in the expression of proinflammatory cytokines. Besides, in response to peritoneal infections, the size and quantity of the omental milky spots (MSs) were increased tremendously and they also support innate-like B1 cell responses and local IgM production in the peritoneal cavity. Furthermore, not only the migration but also the functional activities of neutrophils were diminished in the absence of the omentum. These data collectively show that the omentum contributes more to peritoneal immune responses during septic peritonitis than has heretofore been recognized. Thus, harnessing the function of MS-containing omentum to increase its protective effectiveness may exert important biological and therapeutic implications for the control of intra-abdominal infections.
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http://dx.doi.org/10.3389/fimmu.2021.631609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012523PMC
March 2021

Two-point calibration method for a zoom camera with an approximate focal-invariant radial distortion model.

J Opt Soc Am A Opt Image Sci Vis 2021 Apr;38(4):504-514

A zoom camera can change its focal length and track moving objects with an adjustable resolution. To extract precise geometric information for the tracked objects, a zoom camera requires an accurate calibration method. High-precision camera calibration methods, however, usually require a number of control points that are not guaranteed in some practical situations. Most zoom cameras suffer radial distortion. Athough a traditional method can recover an undistorted image with known intrinsic parameters, it fails to work for a zoom camera with an unknown focal length. Motivated by these problems, we propose a two-point calibration method (TPCM). In this scheme, we first propose an approximate focal-invariant radial distortion (AFRD) model. With the AFRD model, an RGB image can be undistorted with an unknown focal length. After that, the TPCM method is presented to estimate the focal length and rotation matrix with only two control points for one image. Synthetic experiments demonstrate that the AFRD model is efficient. In the real data experiment, the mean reprojection error of the TPCM method is less than one pixel, which is smaller than current state-of-the-art methods, and we believe meets the demand for high-precision calibration.
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http://dx.doi.org/10.1364/JOSAA.414504DOI Listing
April 2021

Mid-infrared electro-optic modulator based on a graphene-embedded plasmonic rib waveguide with ultrahigh electro-optic wavelength tuning.

Appl Opt 2021 Apr;60(10):2795-2802

A graphene-embedded plasmonic rib waveguide (GEPRW) is designed for the mid-infrared electro-optic modulator. The mode characteristics and electro-optic (EO) modulation performances are simulated and optimized by using the finite element method. The results show that propagation length of 10 and figure of merit of 10 are obtained by adjusting the bias voltage applied to the GEPRW. The EO wavelength tunings are -66.69 and -78.87/ for peak and peak in the loss spectra when =3µ and =2µ. For a 100 µm long GEPRW, the modulation depths of ∼96.4,∼97.1,∼93.7, and ∼94.9, and FWHMs of ∼30,∼74,∼34, and ∼59 can be achieved when =1.55, 1.87. 1.89, and 2.23 µm. The EO modulator based on the GEPRW has a wide wavelength tuning range from 1.05 to 2.23 µm. It has high modulation depth, low insertion loss, and broad bandwidth, which can be used as EO tunable devices such as optical interconnects and optical switches.
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http://dx.doi.org/10.1364/AO.419388DOI Listing
April 2021

Ablation of Nampt in AgRP neurons leads to neurodegeneration and impairs fasting- and ghrelin-mediated food intake.

FASEB J 2021 May;35(5):e21450

Integrative Metabolism and Environmental Influences, Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Agouti-related protein (AgRP) neurons in the arcuate nucleus of the hypothalamus regulates food intake and whole-body metabolism. NAD regulates multiple cellular processes controlling energy metabolism. Yet, its role in hypothalamic AgRP neurons to control food intake is poorly understood. Here, we aimed to assess whether genetic deletion of nicotinamide phosphoribosyltransferase (Nampt), a rate-limiting enzyme in NAD production, affects AgRP neuronal function to impact whole-body metabolism and food intake. Metabolic parameters during fed and fasted states, and upon systemic ghrelin and leptin administration were studied in AgRP-specific Nampt knockout (ARNKO) mice. We monitored neuropeptide expression levels and density of AgRP neurons in ARNKO mice from embryonic to adult age. NPY cells were used to determine effects of NAMPT inhibition on neuronal viability, energy status, and oxidative stress in vitro. In these cells, NAD depletion reduced ATP levels, increased oxidative stress, and promoted cell death. Agrp expression in the hypothalamus of ARNKO mice gradually decreased after weaning due to progressive AgRP neuron degeneration. Adult ARNKO mice had normal glucose and insulin tolerance, but exhibited an elevated respiratory exchange ratio (RER) when fasted. Remarkably, fasting-induced food intake was unaffected in ARNKO mice when evaluated in metabolic cages, but fasting- and ghrelin-induced feeding and body weight gain decreased in ARNKO mice when evaluated outside metabolic cages. Collectively, deletion of Nampt in AgRP neurons causes progressive neurodegeneration and impairs fasting and ghrelin responses in a context-dependent manner. Our data highlight an essential role of Nampt in AgRP neuron function and viability.
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http://dx.doi.org/10.1096/fj.202002740RDOI Listing
May 2021