Publications by authors named "Tao Liu"

4,139 Publications

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Electrosynthesis of 2-Substituted Benzoxazoles via Intramolecular Shono-Type Oxidative Coupling of Glycine Derivatives.

J Org Chem 2022 Aug 11. Epub 2022 Aug 11.

College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, People's Republic of China.

Herein, an atom-economical and eco-friendly electrochemical oxidation/cyclization of glycine derivatives through intramolecular Shono-type oxidative coupling is disclosed, leading to a variety of 2-substituted benzoxazoles in 51-85% yields. This oxidative cyclization proceeded in transition metal- and oxidant-free conditions and generated H as only a byproduct. Additionally, gram-scale reactions and a broad substrate scope demonstrated the synthetic usefulness of this protocol.
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http://dx.doi.org/10.1021/acs.joc.2c00856DOI Listing
August 2022

Evaluation of the clinical efficacy and safety of TST33 mega hemorrhoidectomy for severe prolapsed hemorrhoids.

World J Clin Cases 2022 Jun;10(18):6060-6068

Department of Anorectal Surgery, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu 215500, Jiangsu Province, China.

Background: The pathogenesis of hemorrhoids is mainly anal cushion prolapse. Although the traditional treatment has a certain curative effect, it is not ideal. The remission rate of postoperative symptoms is low. Even if temporary remission is achieved, patients with hemorrhoids easily relapse after 1-2 years. The new technique of using staplers to treat prolapsed hemorrhoids has good therapeutic effects in clinical practice.

Aim: To explore the effect of TST33 mega stapler prolapse and hemorrhoid mucosal resection in the treatment of patients with severe prolapsed hemorrhoids.

Methods: A total of 204 patients with severe prolapse hemorrhoids who were admitted to the department of anorectal in our hospital from April 2018 to June 2020 were selected, and the patients were randomly divided into group A and group B with 102 cases in each group using a randomized controlled clinical research program. Patients in Group A were treated with a TST33 mega stapler and hemorrhoid mucosal resection to treat prolapse, and patients in Group B were treated according to the Procedure for Prolapse and Hemorrhoids; the operation time, intraoperative blood loss, hospital stay, the difference in operation time, intraoperative blood loss, hospitalization time, pain degree before and after operation, degree of anal edema, anal Wexner score, and surgical complications were compared between the two groups of patients.

Results: The operation time, intraoperative blood loss and hospitalization time in Group A were significantly lower than those in Group B ( < 0.05). The cure rate of Group A was 98.04%, compared with 95.10% cure rate of Group B, and the difference was not statistically significant ( > 0.05). The visual analogue scale (VAS) at 12 h and 24 h postoperatively in Group A were significantly lower than those in Group B ( < 0.05). The comparison of the VAS scores between Group A and Group B at 48 h, 72 h and 96 h postoperatively revealed that the difference was not statistically significant ( > 0.05). One day postoperatively, the degree of perianal edema in Group A was compared with that in Group B, and the difference was not statistically significant ( > 0.05). Seven days postoperatively, the degree of perianal edema in Group A was significantly lower than that in Group B ( < 0.05). The comparison of anal Wexner scores between the two groups preoperatively and at 1 mo, 3 mo and 6 mo postoperatively showed that the difference was not statistically significant ( > 0.05). The Wexner scores of the two groups at 1 mo, 3 mo and 6 mo postoperatively were significantly lower than the scores preoperatively ( < 0.05). The postoperative complication rate of Group A was 2.94% lower than that of Group B (11.76%), which was statistically significant ( < 0.05).

Conclusion: TST33 mega anastomotic hemorrhoidectomy treatment for patients with severe prolapse hemorrhoids, leads to less postoperative pain, the rapid recovery of perianal edema and has fewer complications.
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http://dx.doi.org/10.12998/wjcc.v10.i18.6060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254172PMC
June 2022

Transport and retention of nano emulsified vegetable oil in porous media: Effect of pore straining, roughness wedging, and interfacial effects.

J Environ Manage 2022 Aug 6;320:115912. Epub 2022 Aug 6.

School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing, 100083, PR China.

Emulsified vegetable oil (EVO), as one of the novel green substrates, has been widely used in subsurface remediation. In these applications, the retention behavior of EVO presents a challenge to remediation efficiency as mechanism insights into the retention of EVO is limited. Herein, Brinell funnels experiments with X-ray microtomography (XMT) were conducted to examine the drainage and retention of nanoscale EVO in porous media, with a specific focus on investigating the impact of pore straining, grain surface roughness, and interfacial effects on Nano-EVO (NEVO) retention. This study demonstrated that the retention of NEVO in porous media is the synergistic result of pore straining, roughness wedging, and interface attachment. With the action of these effects, three residual states of NEVO, incorporating retention at porous ganglia, grain-grain contacts, and grain surface, were identified by XMT in porous media. After multiple periods of drainage and imbibition, the NEVO arrived at stable retention proportions of 46.3%, 72.2%, and 85.9% in three independent systems with coarse, medium, and fine sand as porous media, respectively. The interfacial effects, including the attachment of solid-phase and air-liquid interface, are confirmed as the dominant factors for the retention of NEVO in porous media, which contributed 35.63-47.33% of total retention for the conditions employed. Correspondingly, the contributions of pore straining and roughness wedging only ranged 3.78-24.06% and 3.87-9.94%, respectively. The consistency of the contributions between the actual measurement of XMT and computational evaluation further confirmed the rationality and reliability of the results. In such the dominant factor, interfacial tension, contact angle, and capillary radius play an essential role in NEVO retention, which could be reflected by capillary rise height. These findings advance our understanding on NEVO retention caused by substrate-media interaction and also offer a promising direction for subsurface remediation.
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http://dx.doi.org/10.1016/j.jenvman.2022.115912DOI Listing
August 2022

Bilateral percutaneous kyphoplasty achieves more satisfactory outcomes compared to unilateral percutaneous kyphoplasty in osteoporotic vertebral compression fractures: A comprehensive comparative study.

J Back Musculoskelet Rehabil 2022 Jul 27. Epub 2022 Jul 27.

Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

Background: Osteoporotic vertebral compression fractures (OVCFs) are the most common complication of osteoporosis, a worldwide disease that disturbs the elderly.

Objective: The purpose of the study was to comprehensively compare the clinical efficacy of unilateral percutaneous kyphoplasty (UPKP) and bilateral percutaneous kyphoplasty (BPKP) when treating OVCFs and evaluate their ability to maintain the outcomes in a 2-year follow-up.

Methods: From January 2015 to December 2016 a total of 79 patients with OVCFs were included in the study. They were divided into UPKP group and BPKP group. Subsequently, perioperative data, radiological outcomes, clinical outcomes, and complications were compared between two groups. The follow-up consultation was 3 months, 1 year, and 2 years after the operation.

Results: 37 of patients (14 males, 23 females) were allocated to the UPKP group and 42 patients (13 males, 29 females) were treated with BPKP. The duration of operation and injected cement volume were significantly higher in the BPKP group than those of the UPKP group. BPKP achieved significantly higher improvement in middle height and volume of the fractured vertebral body than UPKP did. There were significantly higher losses of anterior, middle height and volume of the fractured vertebral body in the UPKP group at 2-year follow-up than in the BPKP group. At the final follow-up, Oswestry Disability Index (ODI) of BPKP group was lower than that of UPKP group.

Conclusion: Both UPKP and BPKP achieve satisfactory radiological and clinical outcomes when treating OVCFs. However, in a 2-year follow-up, BPKP maintains vertebral height restoration, volume of vertebral body, and ODI better than UPKP do.
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http://dx.doi.org/10.3233/BMR-210225DOI Listing
July 2022

Social policy reform driven by crises: Promoting and reshaping social policy during the SARS and COVID-19 pandemics in China.

Authors:
Tao Liu

Soc Policy Adm 2022 May 17. Epub 2022 May 17.

School of Public Affairs and Academy of Social Governance Zhejiang University Hangzhou China.

This article focuses on one particular and under-investigated dimension in the study of social policy - crisis events and their special function in promoting and shaping social protection programs. Crises are usually regarded as negative social events that tempestuously challenge the pre-existing socio-economic order, triggering social conflicts and disruption, either temporarily or in a more enduring fashion. However, social crises may also foster new opportunities for social investment, public finance expansion and welfare state building after the outbreak of a crisis and even during the post-crisis period. This article first examines the SARS pandemic and analyzes its developmental trajectory, reconstructing the national debates and discourses on the negative consequences and lessons learned in the SARS and post-SARS period. I argue that this crisis event revealed many loopholes in the Chinese public health system, constituting a powerful driving force for rebuilding the medical insurance system and health governance in China, and strengthening public discourse on welfare state responsibility. Second, I explore how, during the COVID-19 pandemic, scholars and experts have constructed the 'short board' of Chinese social policy, calling for further social policy reforms to narrow the gaps in health protection resources among social classes and to eliminate the fragmentation of health resource distribution.
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http://dx.doi.org/10.1111/spol.12818DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347754PMC
May 2022

Altered Regional Homogeneity in Patients With Diabetic Erectile Dysfunction: A Resting-State fMRI Study.

Front Endocrinol (Lausanne) 2022 22;13:817523. Epub 2022 Jul 22.

Department of Andrology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

Introduction: Erectile dysfunction (ED) is a common complication of Type-2 Diabetes Mellitus (T2DM) for male patients and it is considered to be associated with different causes including hyperglicemia-induced vascular endothelial cell damages. However, the possible central neural mechanisms shared by these two diseases remain unclear. This study aimed to explore the changes of brain activity and their relationships with the clinical characteristics in patients with diabetic ED.

Methods: The data of resting-state functional magnetic resonance imaging were acquired in 31 T2DM patients with ED (DM-ED) and 31 matched healthy controls (HCs). The whole-brain regional homogeneity (ReHo) values were calculated and compared between groups. In addition, correlation analysis was performed to evaluate the relationships between brain regions with altered ReHo values and clinical characteristics in the patient group.

Results: The DM-ED group exhibited increased ReHo values in the right middle frontal gyrus (orbital part) and decreased ReHo values in the left superior frontal gyrus (dorsolateral), paracentral lobule, precuneus and bilateral supplementary motor area when compared with the HCs group. Moreover, significantly negative correlations were found between ReHo values of the left superior frontal gyrus (dorsolateral) and IIEF-5 scores, as well as the level of HbA1c in the DM-ED group.

Conclusion: The altered spontaneous brain activity in cognitive-related regions revealed by ReHo values might provide new insights into the neurological pathophysiology underlying DM-ED and serve as potential neuroimaging biomarkers for detecting and evaluating ED in diabetes patients.
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http://dx.doi.org/10.3389/fendo.2022.817523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355575PMC
August 2022

Two -Methyltransferases Mediate Multiple Methylation Steps in the Biosynthesis of Coumarins in .

J Nat Prod 2022 Aug 5. Epub 2022 Aug 5.

Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

Coumarins with methoxy groups such as osthole (), xanthotoxin (), bergapten (), and isopimpinellin () are typical bioactive ingredients of many medicinal plants. The methylation steps remain widely unknown. Herein, we report the discovery of two methyltransferases in the biosynthesis of -methyl coumarins in by transcriptome mining, heterologous expression, and in vitro enzymatic assays. The results reveal that (i) CmOMT1 catalyzes the methylation of osthenol () as the final step in the biosynthesis of , (ii) CmOMT2 shows the highest efficiency and preference for methylating xanthotoxol () to form , and (iii) CmOMT1 and CmOMT2 also efficiently transform bergaptol () and 8-hydroxybergapten () into or , suggesting the CmOMTs mediate multistep methylations in the biosynthesis of linear furanocoumarins in . .
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http://dx.doi.org/10.1021/acs.jnatprod.2c00410DOI Listing
August 2022

Construction of a N,P doped 3D dendrite-free lithium metal anode by using silicon-containing lithium metal.

Dalton Trans 2022 Aug 4. Epub 2022 Aug 4.

School of Materials Science and Engineering, Beijing Key Laboratory of Environment Science and Engineering, Beijing Higher Institution Engineering Research Center of Power Battery and Chemical Energy Materials, Beijing Institute of Technology, Beijing, 100081, PR China.

Lithium is thought to be an excellent anode material for next-generation Li metal batteries (LMBs). However, some problems with lithium anodes often lead to serious safety concerns and catastrophic failures due to the huge volume change, Li dendritic growth, and related side reactions. Therefore, in order to manufacture stable rechargeable batteries, the abovementioned serious problems must be effectively solved. In this paper, a three-dimensional N,P-doped silicon-containing lithium anode is designed and prepared by metallurgy using low-cost SiN. The 3D stable composite anode (DLi/LiSi CA) was prepared by adding a small amount of SiN to molten lithium to form N-doped silicon-containing lithium metal which was supported on a polyaniline modified carbon cloth (PMCC). The results show that the DLi/LiSi CA not only has high Li affinity but can also effectively inhibit lithium nucleation and lithium dendritic growth, so as to maintain good structural stability in the process of Li plating/stripping. The new lithium metal anode based on doping and 3D carbon cloth shows good cycling stability and low polarizability in both symmetrical and full cells.
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http://dx.doi.org/10.1039/d2dt01387hDOI Listing
August 2022

Ruminal Microbiota Determines the High-Fiber Utilization of Ruminants: Evidence from the Ruminal Microbiota Transplant.

Microbiol Spectr 2022 Aug 4:e0044622. Epub 2022 Aug 4.

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.

The rumen, which contains a series of prokaryotes and eukaryotes with high abundance, determines the high ability to degrade complex carbohydrates in ruminants. Using 16S rRNA gene sequencing, we compared the ruminal microbiota of dairy goats with that in the foregut and colon of mice and found more identified in the rumen, which helps ruminants to utilize plant-derived polysaccharides, cellulose, and other structural carbohydrates. Furthermore, high-fiber diets did not significantly increase intestinal fiber-degrading bacteria in mice, but did produce higher levels of ruminal fiber-degrading bacteria in dairy goats. Through rumen microbe transplantation (RMT), we found that rumen-derived fiber-degrading bacteria can colonize the intestines of mice to exert their fiber-degrading function, but their colonization efficiency is affected by diet. Additionally, the colonization of these fiber-degrading bacteria in the colon may involve higher content of butyrate in the colon, protecting the colonic epithelial barrier and promoting energy metabolism. Overall, the fiber degradation function of rumen bacteria through RMT was verified, and our results provide new insights into isolating the functional and beneficial fiber-degrading bacteria in the rumen, providing a theoretical basis for the role of dietary fiber in intestinal health. Ruminants have a powerful progastric digestive system that converts structural carbohydrates into nutrients useful to humans. It is well known that this phenomenon is due to the fact that the rumen of ruminants is a natural microbial fermenter, which can ferment structural carbohydrates such as cellulose and hemicellulose and transform them into volatile fatty acids to supply energy for host. However, monogastric animals have an inherent disadvantage in utilizing fiber, so screening rumen-derived fiber-degrading bacteria as a fermentation strain for biological feed is needed in an attempt at improving the fiber digestibility of monogastric animals. In this study, a ruminal microbiota transplant experiment from goats to mice proves that ruminal microbiota could serve as a key factor in utilization of high-fiber diets and provides a new perspective for the development of probiotics with fiber degradation function from the rumen and the importance of the use of prebiotics during the intake of probiotics.
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http://dx.doi.org/10.1128/spectrum.00446-22DOI Listing
August 2022

Dietary emulsifier glycerol monodecanoate affects the gut microbiota contributing to regulating lipid metabolism, insulin sensitivity and inflammation.

Food Funct 2022 Aug 4. Epub 2022 Aug 4.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

Glycerol monodecanoate (GMD) is a medium-chain monoacylglycerol that possesses emulsifying and antibacterial properties. The common emulsifiers carboxymethylcellulose and polysorbate-80 have been reported to cause intestinal microbiota dysbiosis and metabolic disturbances. Glycerol monolaurate (GML), another medium-chain monoacylglycerol, is often used as an emulsifier and could improve metabolism by regulating the gut microbiota. However, research on the effects of GMD on the metabolism and gut microbiota remains scarce. Mice were fed a normal chow diet with or without GMD (150, 800, and 1600 mg kg) for 22 weeks. Metabolism indicators and related genes, gut microbiota, and fecal SCFAs were analyzed. The results demonstrated that GMD significantly improved insulin sensitivity, reduced the serum LPS level, and decreased pro-inflammation cytokines including IL-1β, tumor necrosis factor (TNF), and monocyte chemotactic protein 1 (MCP-1). Additionally, 150 and 1600 mg kg GMD could significantly lower the blood glucose content. 1600 mg kg GMD improved cholesterol metabolism and related gene expression compared to 150 and 800 mg kg GMD. Moreover, 150 and 800 mg kg GMD up-regulated the abundance of and , while 1600 mg kg GMD significantly up-regulated the abundance of . Our findings indicated that different doses of GMD had inconsistent effects on lipid metabolism by differentially altering the gut microbiota composition. Meanwhile, all doses of GMD showed excellent effects on increasing insulin sensitivity and improving inflammation.
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http://dx.doi.org/10.1039/d2fo01689cDOI Listing
August 2022

Hydrogen evolution-enabled rhodaelectro-catalyzed [4+2] annulations of purines and 7-deazapurines with alkynes.

Chem Commun (Camb) 2022 Aug 3. Epub 2022 Aug 3.

College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, P. R. China.

A rhodium-catalyzed electrochemical regioselective annulation of 6-phenylpurines and 6-phenyl-7-deazapurines with alkynes has been developed. Electricity is used to recycle the active rhodium-based catalyst, promote the evolution of H and help in reducing the reaction temperature. This mild and green method enables a broad substrate scope and wide functional group tolerance, providing a new series of polycyclic purinium and 7-deazapurinium salts in high yields. Mechanistic studies show that the five-member rhodium(III) species and purinoisoquinolinium-coordinated rhodium(I) complex are the two key intermediates in this transformation.
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http://dx.doi.org/10.1039/d2cc03625hDOI Listing
August 2022

Detection of trh Vibrio parahaemolyticus in seafood by lac dye coloration-based label-free lateral flow immunoassay strip.

J Fish Dis 2022 Aug 3. Epub 2022 Aug 3.

School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, China.

Vibrio parahaemolyticus (V. parahaemolyticus) is an important foodborne pathogen known to cause severe enteric disease. Thus, timely detection of V. parahaemolyticus in seafood is crucial to prevent food poisoning and reduce economic losses. Traditional lateral flow immunoassay strips (LFIS) required good labelling materials and pairing of two antibodies, which made them costly and difficult to manufacture. In this study, a label-free and lac dye coloration-based LFIS (LD-LFIS) to detect trh V. parahaemolyticus was developed for the first time. Lac dye was used to stain V. parahaemolyticus, and LFIS was used to detect stained bacteria. Dimethyl sulphoxide (DMSO) and simultaneous mordanting were chosen as the best solvent and the best staining method for lac dye. In addition, three mordants [KAl(SO ) ·12H O, NH Fe(SO ) ·12H O, and AlCl ·6H O] were selected to improve dyeing efficiency. The detection limit of LD-LFIS was 3.9 × 10  CFU/ml when NH Fe(SO ) ·12H O was used as mordant. Feasibility of the LD-LFIS method was verified by detecting trh V. parahaemolyticus in true and spiked seafood samples. The method developed in this study is expected to reduce restrictions on labelling materials and pairing of two antibodies on LFIS, and proposes a novel idea for the rapid detection of V. parahaemolyticus in seafood.
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http://dx.doi.org/10.1111/jfd.13692DOI Listing
August 2022

The evaluation of coronary microvascular obstruction in patients with STEMI by cardiac magnetic resonance T2-STIR image and layer-specific analysis of 2-dimensional speckle tracking echocardiography combined with low-dose dobutamine stress echocardiography.

Heart Vessels 2022 Aug 1. Epub 2022 Aug 1.

Department of Cardiology, The Affiliated Hospital of Xuzhou Medical University, NO. 99 Huaihai west road, Xuzhou, Jiangsu, China.

This study was designed to assess coronary microvascular obstruction (MVO) in patients with acute ST-segment elevation myocardial infarction (STEMI) by cardiac magnetic resonance T2-weighted short tau inversion recovery (T2-STIR) image and layer-specific analysis of 2-dimensional speckle tracking echocardiography combined with low-dose dobutamine stress echocardiography (LDDSE-LS2D-STE). 32 patients were enrolled to perform cardiac magnetic resonance and echocardiography 5-7 days after primary percutaneous coronary intervention. Infarcted myocardium was categorized into MVO group and MVO group by late gadolinium enhancement as gold standard. At T2-weighted image, the area of hyper-intense region and hypo-intense core inside were marked as A1, A2 and A2/A1 > 0 represented MVO. Strain parameters were composed of longitudinal strain (LS), circumferential strain and radial strain at rest and dobutamine stress. There were 94 MVO segments, 136 MVO segments according to gold standard. 96 segments had hypo-intense core at T2-STIR image. The sensitivity and specificity of T2-STIR in detecting MVO were 91.49 and 92.65%. Endocardial LS was superior to other parameters, and stress endocardial LS was higher than that of resting endocardial LS (sensitivity: 77.11% vs 72.29%, specificity: 93.28% vs 83.19%, AUC: 0.87 vs 0.82, P < 0.05). The combination of T2-STIR and stress endocardial LS in parallel test could improve sensitivity significantly (98.05% vs 91.49%). T2-STIR has higher diagnostic value in detecting MVO with some limitations. However, LDDSE-LS2D-STE with cost-effective and handling may be a good alternative to T2-STIR. It provides additional and reliable diagnostic tools to identify MVO in STEMI patients after reperfusion.
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http://dx.doi.org/10.1007/s00380-022-02131-xDOI Listing
August 2022

Reliability of using generic flow conditions to quantify aneurysmal haemodynamics: A comparison against simulations incorporating boundary conditions measured in vivo.

Comput Methods Programs Biomed 2022 Jul 21;225:107034. Epub 2022 Jul 21.

Macquarie Medical School, Faculty of Medicine, Health, and Human Sciences, Macquarie University, Sydney, NSW 2109, Australia. Electronic address:

Background And Objectives: Initiation, growth, and rupture of intracranial aneurysms are believed to be closely related to their local haemodynamic environment. While haemodynamics can be characterised by use of computational fluid dynamics (CFD), its reliability depends heavily upon accurate assumption of the boundary conditions. Herein, we compared the simulated aneurysmal haemodynamics obtained by use of generic boundary conditions against those obtained under flow conditions measured in vivo.

Methods: We prospectively recruited 19 patients with intracranial aneurysms requiring 3-dimensional rotational angiography, during which blood pressure at the internal carotid artery was probed by catheter and flowrate measured by a dedicated software tool. Using these flow conditions measured in vivo, we quantified the aneurysmal haemodynamics for each patient by CFD, and then compared the results with those derived from a generic condition reported in the literature, in terms of the time-averaged wall shear stress (TAWSS), oscillatory shear index (OSI), relative residence time (RRT), and percentage of the intra-aneurysmal flow (PIAF). In addition, the effects on aneurysmal haemodynamics of different outflow strategies (splitting method vs. Murray's Law) and simulation schemes (transient vs. steady-state) relative to each flow condition were also assessed.

Results: Differences in the simulated TAWSS (-6.08 ± 10.64 Pa, p = 0.001), OSI (0.06 ± 0.13, p = 0.001), and PIAF (-0.05 ± 0.20, p = 0.012) between the patient-specific and generic boundary conditions were found to be statistically significant, in contrast to that in the RRT (49 ± 307 Pa, p = 0.062). Outflow strategies did not yield statistically significant differences in any of the investigated parameters (all p > 0.05); rather, the resulting parameters were found to be in good correlations (all r > 0.71, p < 0.001). Difference between the aneurysmal TAWSS and the WSS derived from cycle-averaged flowrate condition was found to be minor (0.66 ± 1.36 Pa, p = 0.000), so was that between PIAFs obtained respectively from the transient and steady-state simulations (0.02 ± 0.05, p = 0.000).

Conclusions: Incorporating into simulation the patient-specific boundary conditions is critical for CFD to characterise aneurysmal haemodynamics, while outflow strategies may not introduce significant uncertainties. Steady-state simulation incorporating the cycle-averaged flow condition may produce unbiased WSS and PIAF compared to the transient analysis.
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http://dx.doi.org/10.1016/j.cmpb.2022.107034DOI Listing
July 2022

Utilization of 3D Laser Scanning for Stability Evaluation and Deformation Monitoring of Landslides.

J Environ Public Health 2022 21;2022:8225322. Epub 2022 Jul 21.

School of Environmental and Safety Engineering, Changzhou University, Changzhou Jiangsu 213164, China.

Three-dimensional laser scanning technology can comprehensively and accurately monitor slope deformation. To conduct deformation monitoring and stability evaluation of the Changzhou Shunguoshan landslide, in this paper, the causes of the Changzhou Shunguoshan landslide were analyzed. Consequently, 3D laser scanning technology and the traditional monitoring methods such as data from the total station were compared. The point cloud data provides big data support for landslide deformation monitoring and landslide stability early warning. Meanwhile, the landslide stability was evaluated by analogy with existing studies on slope deformation monitoring data. Results show that the three-dimensional laser scanning monitoring data is similar to the total station monitoring data. The overall deformation of the Shunguoshan landslide is no more than ± 0.0015 m; the deformation of the Liyang slope is less than ±0.09 m, which is far less than the analog slope deformation monitoring data. The slope construction and monitoring process are in a stable state.
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http://dx.doi.org/10.1155/2022/8225322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334046PMC
August 2022

Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Atopic Dermatitis Symptoms in Mice through Modulating Immune Balance and Skin Barrier Function.

Mediators Inflamm 2022 21;2022:6206883. Epub 2022 Jul 21.

Department of Dermatology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Atopic dermatitis (AD) is a chronic skin inflammatory disease associated with immune abnormalities and disrupted skin barrier function. Mesenchymal stem cells (MSCs) have been suggested as an alternative therapeutic option in AD. Stem cells from human exfoliated deciduous teeth (SHEDs) are a unique postnatal stem cell population with high immunomodulatory properties. The aim of this study was to explore the effects of SHEDs on AD in the BALB/c mouse model induced by 2,4-dinitrochlorobenzene (DNCB). SHEDs were administrated intravenously or subcutaneously, and clinical severity, histopathological findings, skin barrier function, and organ indexes were evaluated. Skin tissue cytokine mRNA levels and serum cytokine protein levels were further analysed. SHED administration significantly alleviated AD clinical severity, including dermatitis scores, ear thickness, scratching behaviour, and infiltration of mast cells. In addition, disrupted skin barrier function and enlarged spleens were restored by SHED administration. Further, SHED treatment reduced the levels of IgE, IgG1, and thymic stromal lymphopoietin (TSLP) in the serum and the modulated expression of Th1-, Th2-, and Th17-associated cytokines in skin lesions. In conclusion, SHEDs attenuated AD-like skin lesions in mice by modulating the immune balance and skin barrier function. SHEDs could be a potential new treatment agent for AD.
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http://dx.doi.org/10.1155/2022/6206883DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334056PMC
August 2022

Electrospun kaolin-loaded chitosan/PEO nanofibers for rapid hemostasis and accelerated wound healing.

Int J Biol Macromol 2022 Jul 28;217:998-1011. Epub 2022 Jul 28.

Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Tianjin 300457, PR China. Electronic address:

Development of chitosan-based hemostatic products and their application in wound healing has always been a research hotspot in the field of emergency treatment and biomedicine. In this study, the classic hemostatic chitosan and the most well-known inorganic hemostatic agent-kaolin were tried to combine to form a more excellent dressing. Together with the aid of non-toxic, harmless and good hydrophilic polyethylene oxide, chitosan/polyethylene oxide (PEO)/kaolin nanofiber membranes (CPKs) were prepared by electrospinning technology. Such membranes exhibited adjustable mechanical properties and good biocompatibility. Furthermore, a series of in vitro coagulation experiments proved that CPKs with 10 % ratio of kaolin (CPK10) has excellent hemostatic ability. Especially, in the whole blood coagulation time (WBCT) assay, the hemostatic time of CPK10 (43 ± 1.4 s) was significantly lower than that of chitosan/polyethylene oxide (CPK0) nanofiber membrane (61 ± 2.2 s) and QuikClot® Combat Gauze (55.7 ± 1.2 s). The further rat liver injury test reconfirmed that CPK10 can stop bleeding better and faster compared to other groups. In addition, CPKs could promote back wound healing in rats within 14 days without significant inflammatory response. This safe and effective hemostatic CPKs is expected to be a promising candidate hemostat in pre-hospital medical care.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.186DOI Listing
July 2022

The value of urinary gonadotropins in the diagnosis of central precocious puberty: a meta-analysis.

BMC Pediatr 2022 07 28;22(1):453. Epub 2022 Jul 28.

Department of Pediatrics, the First Affiliated Hospital of Nanchang University, Jiangxi, 330006, Nanchang, China.

Background: The gonadotropin-releasing hormone (GnRH) stimulation test is time-consuming, invasive, and costly. However, it is the diagnostic gold standard for central precocious puberty (CPP), which in girls is defined as the onset of secondary sexual characteristics before the age of 8 years accompanied by breast buds, accelerated growth, and advanced bone age. This meta-analysis was performed to compare the diagnostic value of urinary gonadotropins and the GnRH stimulation test for CPP.

Methods: We searched six databases for relevant literature. In accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, we estimated the sensitivity, specificity, area under the summary receiver operating characteristic curve (AUC), and publication bias.

Results: Six eligible trials fulfilled the inclusion criteria. In the meta-analysis of urinary luteinizing hormone (ULH), after excluding the data of one study, we obtained an AUC of 0.90 (sensitivity = 0.81, specificity = 0.85). The meta-analysis of the ULH to urinary follicle-stimulating hormone (UFSH) ratio revealed an AUC of 0.8116 (sensitivity = 0.79, specificity = 0.84).

Conclusion: Both the ULH level and ULH:UFSH ratio are effective and available approaches for CPP diagnosis.

Trial Registration: INPLASY 2021120076 .
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http://dx.doi.org/10.1186/s12887-022-03481-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331156PMC
July 2022

New prediction method of horizontal principal stress of deep tight reservoirs.

Sci Rep 2022 Jul 28;12(1):12908. Epub 2022 Jul 28.

Oilfield Technology Service Company of Xinjiang Oilfield Company, PetroChina, Karamay, Xinjiang, 834000, China.

As the tight reservoir of Lucaogou Formation in Jimsar sag is characterized as large burial depth and poor physical properties, hydraulic fracturing technology is greatly needed to increase the production. Firstly, based on elastic theory, the borehole displacement formula under stress is derived, then the borehole quasi-ellipse characteristic model is proposed, on this basis, the in-situ stress prediction model based on borehole deformation is constructed. Secondly, the force analysis of diamond bit during drilling is performed, and the continuous prediction formula of elastic modulus based on weight on bit (WOB) and torque parameters is derived. Combined with these two steps, a new model of continuous in-situ stress prediction based on borehole deformation and drilling parameters is established. Compared with the values measured by the acoustic emission method in the laboratory, the deviation between the predicted in-situ stress values and those measured by the acoustic emission method is less than 4%, which meets the accuracy requirements of the fracturing in the oil field. The prediction results show that the minimum horizontal principal stress of Lucaogou Formation is 51.1-62.7 MPa, and the maximum horizontal principal stress is 58.9-69.1 MPa, respectively. The maximum horizontal principal stress direction is NW-SE direction with an angle of about 159°. The results show that the fracture direction in the study area is in accordance with the present in-situ stress direction, which is beneficial to the secondary reconstruction of natural fractures and keeps good opening property of fractures. This study provides a theoretical basis for in-situ stress prediction and compressibility evaluation of tight reservoir.
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http://dx.doi.org/10.1038/s41598-022-16954-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334574PMC
July 2022

Photoreceptor and Retinal Pigment Epithelium Relationships in Eyes With Vitelliform Macular Dystrophy Revealed by Multimodal Adaptive Optics Imaging.

Invest Ophthalmol Vis Sci 2022 Jul;63(8):27

National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.

Purpose: To assess the structure of cone photoreceptors and retinal pigment epithelial (RPE) cells in vitelliform macular dystrophy (VMD) arising from various genetic etiologies.

Methods: Multimodal adaptive optics (AO) imaging was performed in 11 patients with VMD using a custom-assembled instrument. Non-confocal split detection and AO-enhanced indocyanine green were used to visualize the cone photoreceptor and RPE mosaics, respectively. Cone and RPE densities were measured and compared across BEST1-, PRPH2-, IMPG1-, and IMPG2-related VMD.

Results: Within macular lesions associated with VMD, both cone and RPE densities were reduced below normal, to 37% of normal cone density (eccentricity 0.2 mm) and to 8.4% of normal RPE density (eccentricity 0.5 mm). Outside of lesions, cone and RPE densities were slightly reduced (both to 92% of normal values), but with high degree of variability in the individual measurements. Comparison of juxtalesional cone and RPE measurements (<1 mm from the lesion edge) revealed significant differences in RPE density across the four genes (P < 0.05). Overall, cones were affected to a greater extent than RPE in patients with IMPG1 and IMPG2 pathogenic variants, but RPE was affected more than cones in BEST1 and PRPH2 VMD. This trend was observed even in contralateral eyes from a subset of five patients who presented with macular lesions in only one eye.

Conclusions: Assessment of cones and RPE in retinal locations outside of the macular lesions reveals a pattern of cone and RPE disruption that appears to be gene dependent in VMD. These findings provide insight into the cellular pathogenesis of disease in VMD.
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http://dx.doi.org/10.1167/iovs.63.8.27DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344216PMC
July 2022

Bacillus coagulans in Combination with Chitooligosaccharides Regulates Gut Microbiota and Ameliorates the DSS-Induced Colitis in Mice.

Microbiol Spectr 2022 Jul 28:e0064122. Epub 2022 Jul 28.

Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu Universitygrid.411292.d, Chengdu, People's Republic of China.

Ulcerative colitis (UC) are chronic inflammatory disorders, which may be caused by intestinal barrier dysfunction, immune system disorders and intestinal microbiota dysbiosis. Synbiotic, the combination of probiotics and prebiotics, is thought to be a pragmatic approach in mitigating inflammation in UC. Bacillus coagulans has been recognized as a potential probiotic for treating intestinal diseases because of its favorable industrial and probiotic properties, including sporulation and lactic acid production. In this study, we evaluated the treatment effects of the FCYS01 spores with or without the chitooligosaccharides (COSs) on UC generated using dextran sulfate sodium (DSS) in mice. Supplementation of spores, prebiotic COSs or the synbiotic (the spores + COSs) had a significant positive effect on DSS-induced UC. The disease activity index and histological damage score were significantly reduced after these supplementations. Compared to DSS group, these supplementations also significantly modulated the cytokines IL-4, IL-6, IL-8, IL-10, and C-reactive protein (CRP) levels and significantly maintained expressions of tight junction proteins and mucin protein and promotes recovery of the intestinal barrier. In addition, these supplementations regulate the composition of gut microbiota and improve the production of short-chain fatty acids (SCFAs), through enrichment of SCFA-producing bacteria, such as and species. In summary, the synbiotic ameliorated the overall inflammatory status of the experimental UC model and showed a better treatment effect than or COSs did alone as revealed by the markers such as, colon length, IL-4 and Occludin levels. Probiotic and prebiotic are believed to be useful in alleviating the inflammatory, thereby resolving or preventing the severity of UC. Spore-forming bacteria Bacillus coagulans show advantages of stability and probiotic effects, being suggested as the important probiotics for UC treatment. Here, we demonstrate that administration of spores, chitooligosaccharides (COSs), or the synbiotic attenuates DSS-induced colitis and significantly correlates with altered gut immune responses. The treatment effect of the synbiotic is inferred to be relied on the enrichment of probiotic bacteria, such as and species, which are reported to be crucial important for gut health. Our findings facilitate the development of therapeutic and preventive strategies for UC using spore-forming lactic acid bacteria in combination with COSs.
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http://dx.doi.org/10.1128/spectrum.00641-22DOI Listing
July 2022

A Novel Circularly Polarized Folded Transmitarray Antenna with Integrated Radiation and Scattering Performance.

Sensors (Basel) 2022 Jul 23;22(15). Epub 2022 Jul 23.

Information and Navigation College, Air Force Engineering University, Xi'an 710077, China.

In this paper, a novel design method of circularly polarized folded transmitarray antenna (CPFTA) is presented by applying sequential rotation technology. Compared with the general design method, the novel design method can reduce the design difficulty and improve the axis ratio (AR) bandwidth significantly without adding any additional structure. To verify the proposed method, both a general CPFTA (GCPFTA) and a novel CPFTA (NCPFTA) are designed, fabricated, measured and compared. Good agreements between simulated and measured results are obtained. Thanks to the creative design, the integrated radiation and scattering control of the CPFTA is realized for the first time. The proposed NCPFTA has the advantages of broadband, high gain, planar structure, low profile, convenience in installation and low radar cross section (RCS), which has potential application in mobile satellite communication.
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http://dx.doi.org/10.3390/s22155503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332254PMC
July 2022

A Differentiation-Related Gene Prognostic Index Contributes to Prognosis and Immunotherapy Evaluation in Patients with Hepatocellular Carcinoma.

Cells 2022 Jul 26;11(15). Epub 2022 Jul 26.

School of Clinical Medicine, Guizhou Medical University, Guiyang 550000, China.

Hepatocellular carcinoma (HCC) is the most common gastrointestinal tumor with a poor prognosis, which is associated with poor differentiation of tumor cells. However, the potential value of cell differentiation-related molecules in predicting the benefit and prognosis of immune checkpoint inhibitors (ICI) therapy remains unknown. Herein, to investigate the differentiation trajectory of HCC cells and their clinical significance, a differentiation-related gene prognostic index (DRGPI) based on HCC differentiation-related genes (HDRGs) was constructed to elucidate the immune characteristics and therapeutic benefits of ICI in the HCC subgroup defined by DRGPI. Single-cell RNA sequencing (scRNA-seq) and bulk RNA-seq data from four HCC samples were integrated for bioinformatics analysis. Then, PON1, ADH4, SQSTM1, HSP90AA1, and STMN1 were screened out to construct a DRGPI. More intriguingly, RT-qPCR validation of the expression of these genes yielded consistent results with the TCGA database. Next, the risk scoring (RS) constructed based on DRGPI suggested that the overall survival (OS) of the DRGPI-high patients was significantly worse than that of the DRGPI-low patients. A nomogram was constructed based on DRGPI-RS and clinical characteristics, which showed strong predictive performance and high accuracy. The comprehensive results indicated that a low DRGPI score was associated with low TP53 mutation rates, high CD8 T cell infiltration, and more benefit from ICI therapy. Homoplastically, the high DRGPI score reflected the opposite results. Taken together, our study highlights the significance of HCC cell differentiation in predicting prognosis, indicating immune characteristics, and understanding the therapeutic benefits of ICI, and suggests that DRGPI is a valuable prognostic biomarker for HCC.
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http://dx.doi.org/10.3390/cells11152302DOI Listing
July 2022

Correlation between Irradiation Treatment and Metabolite Changes in (Diptera: Tephritidae) Larvae Using Solid-Phase Microextraction (SPME) Coupled with Gas Chromatography-Mass Spectrometry (GC-MS).

Molecules 2022 Jul 20;27(14). Epub 2022 Jul 20.

Institute of Equipment Technology, Chinese Academy of Inspection and Quarantine, No. A3, Gaobeidianbeilu, Chaoyang District, Beijing 100123, China.

The metabolites produced by the larvae of (Diptera: Tephritidae) exposed to different doses of irradiation were analyzed using solid phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS), and a metabonomic analysis method of irradiated insects based on GC-MS was established. The analysis revealed 67 peaks, of which 23 peaks were identified. The metabolites produced by larvae treated with different irradiation doses were compared by multivariate statistical analysis, and eight differential metabolites were selected. Irradiation seriously influenced the fatty acid metabolic pathway in larvae. Using the R platform combined with the method of multivariate statistical analysis, changes to metabolite production under four irradiation doses given to larvae were described. Differential metabolites of larvae carried chemical signatures that indicated irradiation dose, and this method is expected to provide a reference for the detection of irradiated insects.
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http://dx.doi.org/10.3390/molecules27144641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320597PMC
July 2022

Source/Drain Trimming Process to Improve Gate-All-Around Nanosheet Transistors Switching Performance and Enable More Stacks of Nanosheets.

Micromachines (Basel) 2022 Jul 8;13(7). Epub 2022 Jul 8.

Primarius Technologies Co., Ltd., Shanghai 201306, China.

A new S/D trimming process was proposed to significantly reduce the parasitic RC of gate-all-around (GAA) nanosheet transistors (NS-FETs) while retaining the channel stress from epitaxy S/D stressors at most. With optimized S/D trimming, the 7-stage ring oscillator (RO) gained up to 27.8% improvement of delay with the same power consumption, for a 3-layer stacked GAA NS-FETs. Furthermore, the proposed S/D trimming technology could enable more than 4-layer vertical stacking of nanosheets for GAA technology extension beyond 3 nm CMOS technology.
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http://dx.doi.org/10.3390/mi13071080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321815PMC
July 2022

The Value of Cardiopulmonary Exercise Testing in Predicting the Severity of Coronary Artery Disease.

J Clin Med 2022 Jul 18;11(14). Epub 2022 Jul 18.

Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

There have been a limited number of quantitative studies on the relationship between coronary artery disease (CAD) and cardiorespiratory fitness (CRF), as measured by cardiopulmonary exercise testing (CPET). Thus, we aimed to investigate the association between CRF and the severity of coronary artery disease from the most comprehensive perspective possible, and to affirm the predictive value of CPET in the severity assessment of CAD. Our study included 280 patients with coronary angiography, who had undergone CPET in Tongji Hospital. The patients' CRF was measured through their peak oxygen uptake ([email protected]), their oxygen uptake at the anaerobic threshold ([email protected]) and through other parameters of CPET on a bicycle ergometer. The severity of the coronary artery disease was assessed in the following three layers: functionally significant lesions (quantitative flow ratio [QFR] ≤ 0.8), the number of stenotic coronary arteries (SCA, stenosis ≥ 50%) and the Gensini score. The correlation analyses were carried out between the CRF and the severity of the coronary artery disease. A ROC curve was plotted, and the AUC was calculated to distinguish the severe CAD and the non-severe CAD patients, as measured by the QFR, the number of SCA, and the Gensini score. The [email protected] and [email protected] were inversely associated with the QFR. The [email protected], [email protected] and VO/[email protected] were associated with the number of SCA. Meanwhile, the [email protected], VO/[email protected], [email protected] and VO/[email protected] were associated with the Gensini score. An ROC analysis proved that a combination of traditional clinical risk factors and the [email protected]/VO is valuable in predicting CAD severity. Our study demonstrated a strong and inverse association between CRF and the severity of CAD. A combination of traditional clinical risk factors and CRF is valuable in predicting CAD severity.
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http://dx.doi.org/10.3390/jcm11144170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320309PMC
July 2022

Percutaneous Kyphoplasty in Patients with Severe Osteoporotic Vertebral Compression Fracture with and without Intravertebral Cleft: A Retrospective Comparative Study.

Int J Gen Med 2022 18;15:6199-6209. Epub 2022 Jul 18.

Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, 215006, People's Republic of China.

Purpose: To comparatively analyze the clinical and radiographic outcomes of percutaneous kyphoplasty (PKP) in patients with severe osteoporotic vertebral compression fracture (sOVCF) with or without intravertebral cleft (IVC).

Methods: We enrolled a total of 75 patients with sOVCF receiving PKP between January 2016 and December 2018. The patients were divided into the following two groups based on their radiographic findings: with IVC (IVC group) and without IVC (NIVC group). The following radiographic outcomes were determined: anterior vertebral height (AVH), kyphotic angle (KA), lumbar lordosis (LL), sacral slope (SS), pelvic incidence (PI), and pelvic tilt (PT). The clinical functional assessment included Oswestry disability index (ODI) and visual analog scale (VAS) scores.

Results: No significant difference was found between the demographic data of the two groups ( > 0.05). AVH, KA, and LL in both groups were significantly corrected one month after surgery ( < 0.05). There was statistical difference between the two groups in AVH and KA one year and three years after surgery, and in LL and PT three years after surgery ( < 0.05). Compared with the results one month after surgery, AVH, KA, and LL of the IVC group deteriorated significantly one year and three years after surgery, whereas AVH, KA, and LL of the NIVC group deteriorated significantly three years after surgery ( < 0.05). The VAS and ODI scores in both groups decreased significantly one month, one year, and three years after surgery than preoperative results ( < 0.05), and a statistical difference was observed between the two groups three years after surgery ( < 0.05).

Conclusion: PKP can give satisfactory outcomes for the treatment of sOVCF with or without IVC. However, the NIVC group showed better clinical outcomes and could maintain spinal sagittal balance better than the IVC group during long-term follow-up.
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http://dx.doi.org/10.2147/IJGM.S369840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307916PMC
July 2022

Polygonatum Polysaccharide Regulates Macrophage Polarization and Improves LPS-Induced Acute Lung Injury through TLR4-MAPK/NF-B Pathway.

Can Respir J 2022 14;2022:2686992. Epub 2022 Jul 14.

Department of Thoracic Surgery, Changhai Hospital, Shanghai 200433, China.

Objective: To investigate the effects of polygonatum sibiricum polysaccharides (PSPs) on the polarization of macrophages to M1 and M2 phenotypes and their potential mechanism.

Methods: PSPs samples were prepared through water extraction and alcohol precipitation assay. The properties of PSPs were identified and analyzed by high-performance liquid chromatography, FT-IR, and NMR assay. Then, the effects of PSPs on mouse macrophage RAW264.7 viability were measured by CCK-8 assay. The cells were randomly divided into the control group, PSPs group, LPS group, and LPS + PSPs group. M1 phenotype polarization of RAW264.7 cells was induced by LPS treatment. The effects of various treatments on expression of M2 phenotype CD206, activation of TLR4-MAPK/NF-B signal pathway, and translocation of NF-B into the nucleus were determined by ELISA, western blot, and immunofluorescence assay, respectively. TLR4 inhibitor, TAK-242, and MAPK inhibitor, BIRB 796, were used to verify the effects of PSPs on the TLR4-MAPK/NF-B pathway. The mice model of acute lung injury (ALI) was established and randomly divided into control group, PSPs group, LPS group, and LPS + PSPs group. Bronchoalveolar lavage fluid (BALF) and lung tissue were collected to measure protein, inflammatory cells, neutrophil and macrophage cells number, and the levels of IL-6 and TNF- in BALF. Flow cytometry and western blot assay measured the phenotypic changes of macrophages and the activation of the TLR4-MAPK/NF-B signaling pathway.

Results: The concentrations of PSPs lower than 100 g/mL showed no toxicity to RAW264.7 cells. PSPs treatment could significantly reverse the reduction of CD206 protein expression ( < 0.05) and the increase of the expression of inflammatory factor TNF-, IL-1, and IL-6 (all < 0.05), TLR4-MAPK/NF-B signaling pathway activation (all < 0.05), and NF-B translocation into the nucleus induced by LPS. The effect of inhibitors TAK-242 and BIRB 796 was consistent with that of PSPs. In the mice model of ALI, PSPs treatment could reduce the total protein levels of BALF and the number of inflammatory cells level, reverse the number changes of neutrophils and macrophages, and downregulate the proinflammatory factors IL-6 and TNF- caused by LPS (all < 0.05). In addition, PSPs treatment could also significantly reverse the increase in the number of iNOS expressing macrophages in alveolar lavage fluid induced by LPS ( < 0.05). In contrast, CD206-expressed cells decreased ( < 0.05). PSPs could also reverse LPS-induced TLR4-MAPK/NF-B signal pathway protein activation (all < 0.05).

Conclusion: PSPs could suppress TLR4-MAPK/NF-B activation induced by LPS, inhibit M1 phenotypic polarization of macrophages, and promote M2 phenotypic polarization, thus playing an anti-inflammatory role.
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http://dx.doi.org/10.1155/2022/2686992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303503PMC
July 2022

Wood-Derived Monolithic Catalysts with the Ability of Activating Water Molecules for Oxygen Electrocatalysis.

Small 2022 Jul 24:e2202725. Epub 2022 Jul 24.

College of Chemistry, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, P. R. China.

Oxygen reduction reaction (ORR) is the key reaction on cathode of rechargeable zinc-air batteries (ZABs). However, the lack of protons in alkaline conditions limits the rate of ORR. Herein, an activating water strategy is proposed to promote oxygen electrocatalytic activity by enhancing the proton production from water dissociation. FeP nanoparticles (NPs) are coupled on N-doped wood-derived catalytically active carbon (FeP-NWCC) to associate bifunctional active sites. In alkaline, FeP-NWCC possesses outstanding catalytic activities toward ORR (E  = 0.86 V) and Oxygen evolution reaction (OER) (overpotential is 310 mV at 10 mA cm ). The liquid ZABs assembled by FeP-NWCC deliver superior peak power density (144 mW cm ) and cycle stability (over 450 h). The quasi-solid-state ZABs based on FeP-NWCC also display excellent performances. Theoretical calculation illustrates that the superb bifunctional performance of FeP-NWCC results from the elevated dissociation efficiency of water via FeP NPs to assist the oxygen catalytic process. The strategy of activating water provides a new perspective for the design of ORR/OER bifunctional catalysts. This work is a model for the application of forest biomass.
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http://dx.doi.org/10.1002/smll.202202725DOI Listing
July 2022

Feasibility and positive effects of scalp acupuncture for modulating motor and cerebral activity in Parkinson's disease: A pilot study.

NeuroRehabilitation 2022 Jul 19. Epub 2022 Jul 19.

Department of Acupuncture, Zhejiang Hospital, Hangzhou, China.

Background: A variety of acupuncture therapies have shown efficacy in Parkinson's disease (PD).

Objective: To evaluate scalp acupuncture (SA) effects on motor and cerebral activity by using gait equipment and resting-state functional magnetic resonance imaging (rs-fMRI).

Methods: Twelve patients with PD received SA. They underwent the first functional-imaging scan after tactile stimulation and the second scan following needle removal. Gait test and local sensation assessment were performed immediately after each functional scan. Gait parameter differences between pre- and post-SA were analyzed using a paired t-test and altered brain areas in degree centrality (DC) and fractional amplitude of low-frequency fluctuation (fALFF) were identified between the two scans.

Results: Eight patients completed the experiment. Stride length, maximum ankle height, maximum ankle horizontal displacement, gait speed, and range of shank motion significantly increased post-treatment (P <  0.05). fALFF in left middle frontal gyrus and DC in left cerebellum (corrected) increased, while fALFF in left inferior parietal lobule (corrected) during SA decreased, compared with those in tactile stimulation. A positive correlation was observed between right limb swings and both fALFF areas.

Conclusions: Differences in gait and brain analyses presented modulation to motor and brain activity in PD, thus, providing preliminary evidence for SA efficacy.
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http://dx.doi.org/10.3233/NRE-220048DOI Listing
July 2022
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