Publications by authors named "Tao Liang"

570 Publications

Incidence of Workplace Violence against Nurses among Chinese Hospitals: A Meta-analysis.

J Nurs Manag 2021 Jul 22. Epub 2021 Jul 22.

School of Nursing, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Aims: To explore the incidence of workplace violence against nurses in Chinese hospitals.

Background: Previous systematic reviews on the incidence of workplace violence against Chinese healthcare workers did not include many articles published in Chinese. Although several studies have investigated cases of violence against healthcare providers in China, no meta-analysis has been conducted to assess the incidence of violence against Chinese nurses.

Evaluation: In this study, relevant data were retrieved from studies published up to July 2020. A meta-analysis was conducted using R software (Version 4.0).

Key Findings: The 12-month incidence of workplace violence among Chinese nurses was 71% (95% CI 67% to 75%) and verbal violence was the most common sub-type of violence (63% 95% CI 58% to 67%).

Conclusion: Chinese nurses are at a high risk of violence at workplace. Hospital managers should explore ways to reduce violence against their employees, especially the younger nurses who work in secondary hospitals.

Implications For Nursing Management: The findings of this study highlight the need to enhance the legal system in terms of laws meant to effectively mitigate violence against nurses in Chinese hospitals. Measures should be particularly taken to protect younger nurses who work in secondary hospitals.
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http://dx.doi.org/10.1111/jonm.13427DOI Listing
July 2021

Long-term ecological effects of two artificial forests on soil properties and quality in the eastern Qinghai-Tibet Plateau.

Sci Total Environ 2021 Jul 9;796:148986. Epub 2021 Jul 9.

Key Laboratory of Land Surface Pattern and Simulation, Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China.

Afforestation is an essential process of ecological restoration, landscape reconstruction, and environmental improvement. While large-scale plantations have restored the fragile ecosystems of the Qinghai-Tibet Plateau, they have also changed local soil characteristics. A 30-year-old typical planted forest on the eastern Qinghai-Tibet Plateau was selected to determine the long-term ecological effects of artificial forests on the soil in this study. Physicochemical soil characteristics at varying soil depths and relative soil parameters, such as element stoichiometry and growing stock, were quantified on the different plantations. This soil quality information was used to construct an MDS-SQI Model. Our findings revealed that soil TN, TK, TP, and AP content was higher than pre-afforestation baseline values, while SOC and pH values were lower. Amounts of soil nutrients SOC, TN, TP, TK, AP, and AK, were positively correlated in the artificial forests. The ratio of soil C/N was higher and ratios C/P and N/P were lower in poplar than the Chinese pine plantation. The soil quality index values calculated from the MDS model were 0.31 and 0.40 for poplar and Chinese pine plantations in the top 30 cm and 0.55 and 0.46 in the 100 cm depth, respectively, which indicated that the two plantations had low-quality soil. LiDAR satellite imagery was used to estimate a growing stock of 7723 m and 435 m in the poplar and Chinese pine plantations. The results suggest that the artificial forest improves soil properties overall but that different stand forests have discrete effects on the soil environment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148986DOI Listing
July 2021

Synergic Neuroprotection Between Hort and Borneol Against Ischemic Stroke by Neurogenesis Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier.

Front Pharmacol 2021 16;12:666790. Epub 2021 Jun 16.

Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, China.

Hort (LCH) is a famous ethnomedicine in Asia known for its excellent output on stroke treatment, and borneol usually acts as an assistant for its reducing permeability of the blood-brain barrier (BBB) after stroke. Although their synergy against brain ischemia was verified in previous studies, the potential mechanism is still unknown. The research aimed to explore the exact synergic mechanisms between LCH and borneol on neurogenesis within the areas of the dentate gyrus and subventricular zone. After treating middle cerebral artery occlusion rats with LCH (0.1 g/kg) and/or borneol (0.08 g/kg), the neurological severity score, brain infarct ratio, Nissl staining, Evans blue permeability, BBB ultrastructure, and expressions of von Willebrand factor and tight junction-associated proteins were measured. Co-localizations of Nestin/BrdU and doublecortin/BrdU, and expressions of neuronal nuclei (NeuN) and glial fibrillary acidic protein (GFAP) were observed under a fluorescence microscope. Moreover, astrocyte polarization markers of complement component 3 and pentraxin 3, and relevant neurotrophins were also detected by immunoblotting. Basically, LCH and borneol had different focuses, although both of them decreased infarct areas, and increased quantity of Nissl bodies and expression of brain-derived neurotrophic factor. LCH increased the neurological severity score, NeuN cells, and the ratios of Nestin/BrdU and doublecortin/BrdU, and decreased GFAP cells and ciliary neurotrophic factor expression. Additionally, it regulated the expressions of complement component 3 and pentraxin 3 to transform astrocyte phenotypes. Borneol improved BBB ultrastructure and increased the expressions of von Willebrand factor, tight junction-associated proteins, vascular endothelial growth factor, and vascular endothelial growth factor receptor 2. Unexpectedly, their combined therapy showed more obvious regulations on the Nissl score, Evans blue permeability, doublecortin/BrdU, NeuN cells, brain-derived neurotrophic factor, and vascular endothelial growth factor than both of their monotherapies. The results indicated that LCH and borneol were complementary to each other in attenuating brain ischemia by and large. LCH mainly promoted neural stem cell proliferation, neurogenesis, and mature neuron preservation, which was probably related to the transformation of reactive astrocytes from A1 subtype to A2, while borneol preferred to maintain the integrity of the BBB, which provided neurogenesis with a homeostatic environment.
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http://dx.doi.org/10.3389/fphar.2021.666790DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242197PMC
June 2021

Sexual size dimorphism in lizards: Rensch's rule, reproductive mode, clutch size, and line fitting method effects.

Integr Zool 2021 Jul 3. Epub 2021 Jul 3.

College of Animal Science, Xinjiang Agricultural University, Urumqi, Xinjiang, China.

Rensch's rule relates to a pattern whereby sexual size dimorphism (SSD) is more female-biased in small-sized species and more male-biased in large-sized ones. We collected literature and museum data on the body size of males and females belonging to 4,032 lizard species, as well as data on their reproductive modes and clutch sizes. We used phylogenetic comparative analyses, and general linear mixed models, to test Rensch's rule and examined how reproductive mode and clutch size affect sexual size dimorphism. Sexual size dimorphism was independent of clutch size in lizard species with variable clutch sizes and in oviparous lizards. Large litters were associated with female-biased sexual dimorphism in viviparous and in scincomorph lizards. Inference regarding Rensch's rule depended on the analytical method used to identify it. The widely used, but less conservative, reduced major axis regression usually support Rensch's rule while ordinary least squares regressions mostly show isometric relationships. The rule tended to apply more to oviparous than viviparous lizards. We infer that Rensch's rule is, at best, a weak pattern in lizards. This is especially true in viviparous lineages, where females reproduce infrequently, therefore evolve large sizes to maximise fecundity, resulting in female-biased dimorphism. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1749-4877.12569DOI Listing
July 2021

Quantifying the Compressive Force of 3D Cardiac Tissues via Calculating the Volumetric Deformation of Built-In Elastic Gelatin Microspheres.

Adv Healthc Mater 2021 Jul 1:e2001716. Epub 2021 Jul 1.

Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering, Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.

Quantifying cardiac contractile force is of paramount important in studying mechanical heart failure and screening therapeutic drugs. However, most existing methods can only measure the in-plane component of twitch force of cardiomyocytes, such that mismatching the centripetal compressive stress of heart beating in physiology. Here, a non-destructive method is developed for quantifying the compressive stress and mapping the distribution of the local stress within the 3D cardiac tissues. In detail, elastic gelatin microspheres labeled with fluorescence beads are fabricated by microfluidic chips with high throughput, and they serve as built-in pressure sensors which are wrapped by cardiomyocytes in 3D tissues. The deformation of microspheres and the displacements of fluorescent beads induced by the contraction of cardiomyocytes are demonstrated to characterize the amount and distribution of the centripetal compressive stress. Further, the method shows a potent capability to locally quantify contractile force variation of 3D cardiac tissues, which is induced by agonist (norepinephrine) and inhibitor (blebbistatin). On the whole, the method significantly improves the 3D measurement of mechanical force in vitro and provides a solution for locally quantifying the compressive stress within engineered cardiac tissues.
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http://dx.doi.org/10.1002/adhm.202001716DOI Listing
July 2021

Prophylactic Norepinephrine Infusion Reduces Postoperative Complications and Hospitalization Time in Elderly Patients Undergoing Posterior Lumbar Spinal Fusion.

Biomed Res Int 2021 3;2021:2161036. Epub 2021 Jun 3.

Department of Anesthesiology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China.

This single-center prospective randomized controlled trial explores the effect of prophylactic norepinephrine infusion on the incidence of complications and hospitalization time in elderly patients (60-85 years old) undergoing posterior lumbar spinal fusion. In total, 129 elderly patients were randomized into two groups: a group that received norepinephrine during general anesthesia and a control group not receiving norepinephrine. The primary outcomes were in-hospital complications and 90-day postoperative complications and hospitalization time. The results show that in-hospital complications occurred in 24 of 60 patients (40%) in the control group versus 11 of 60 patients (18.3%) in the norepinephrine group (RR, 2.182; 95% CI, 1.177-4.045; = 0.015). Cardiac events occurred significantly more frequently in the control than in the norepinephrine group. Total number of patients experiencing complications within 90 days postoperatively was lower in the norepinephrine (11 of 60; 18.3%) than in the control group (26 of 60; 43.3%; RR, 2.364; 95% CI, 1.288-4.339; = 0.005). The median length of hospital stay was 17 days (11-27) in the control group and 15 days (10- 23) in the norepinephrine group ( = 0.01). The secondary outcomes were serum levels of syndecan-1, hyaluronic acid, heparan sulfate, and brain natriuretic peptide. Logistic regression analysis is used to describe the relationship between selected independent variables and in-hospital complications. Intraoperative total fluid, crystalloid, and colloid volumes were significantly higher in the control than in the norepinephrine group. The patients in the norepinephrine group had a higher MAP but a lower heart rate than those in the control group after the induction of anesthesia and intraoperatively. Syndecan-1, hyaluronic acid, and heparan sulfate serum levels showed a different course in the two groups. In conclusion, prophylactic norepinephrine infusion during posterior lumbar spinal fusion is preferable for elderly patients undergoing lumbar spinal fusion under general anesthesia. It can reduce postoperative complications and hospitalization time by reducing the injury to the vascular endothelium. This trial is registered with Clinical Trial Registration http://www.chictr.org.cn/showproj.aspx?proj=33660, identifier ChiCTR-1900021309.
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http://dx.doi.org/10.1155/2021/2161036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195642PMC
June 2021

Degradation of high concentration starch and biocathode autotrophic denitrification using photo microbial fuel cell.

Chemosphere 2021 Oct 3;280:130776. Epub 2021 May 3.

Key Laboratory of Coal Science and Technology of Shanxi Province and Ministry of Education, Taiyuan University of Technology, Shanxi, 030024, China.

In the study, a dual-chamber photo MFC was constructed with a photosynthetic bacteria consortium PB-Z and a heterotrophic nitrifier C16 as anode and cathode inoculant, respectively. The electron released from starch degradation in the anode by photosynthetic bacteria was transferred to the cathode, which was utilized by the nitrifying bacteria C16 to realize autotrophic denitrification. Lower resistance was more conducive to the electron transfer and pollutants removal. Comparing with natural light, continuous light greatly promoted starch degradation by the photosynthetic bacteria in the anode and the denitrification by the nitrifying bacteria in the cathode. Under continuous light and external resistance of 500 Ω, high concentration starch was degraded by photosynthetic bacteria PB-Z and the COD removal efficiency reached up to 88.45% within 12 d, and nitrate of 95.8% was removed within 4 d by autotrophic denitrification by heterotrophic nitrifier C16. The study provides some enlightenment and reference for the application of MFC in the field of wastewater treatment.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130776DOI Listing
October 2021

Structural basis for CSPG4 as a receptor for TcdB and a therapeutic target in Clostridioides difficile infection.

Nat Commun 2021 06 18;12(1):3748. Epub 2021 Jun 18.

Department of Physiology and Biophysics, University of California, Irvine, CA, USA.

C. difficile is a major cause of antibiotic-associated gastrointestinal infections. Two C. difficile exotoxins (TcdA and TcdB) are major virulence factors associated with these infections, and chondroitin sulfate proteoglycan 4 (CSPG4) is a potential receptor for TcdB, but its pathophysiological relevance and the molecular details that govern recognition remain unknown. Here, we determine the cryo-EM structure of a TcdB-CSPG4 complex, revealing a unique binding site spatially composed of multiple discontinuous regions across TcdB. Mutations that selectively disrupt CSPG4 binding reduce TcdB toxicity in mice, while CSPG4-knockout mice show reduced damage to colonic tissues during C. difficile infections. We further show that bezlotoxumab, the only FDA approved anti-TcdB antibody, blocks CSPG4 binding via an allosteric mechanism, but it displays low neutralizing potency on many TcdB variants from epidemic hypervirulent strains due to sequence variations in its epitopes. In contrast, a CSPG4-mimicking decoy neutralizes major TcdB variants, suggesting a strategy to develop broad-spectrum therapeutics against TcdB.
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http://dx.doi.org/10.1038/s41467-021-23878-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213806PMC
June 2021

[Arthroscopy combined with high tibial osteotomy for the treatment of knee medial compartment osteoarthritis and its influence on cartilage injury].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Jun;35(6):690-696

Department of Orthopedics, Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou Sichuan, 646000, P.R.China.

Objective: To investigate the effectiveness of arthroscopy combined with high tibial osteotomy (HTO) in the treatment of knee medial compartment osteoarthritis and its influence on cartilage injury.

Methods: The clinical data of 57 patients with knee medial compartment osteoarthritis treated with arthroscopy combined with HTO between March 2017 and March 2019 were retrospectively analyzed. There were 27 males and 30 females with an average age of 52.4 years (range, 44-57 years). The disease duration ranged from 3 to 6 years, with an average of 3.6 years. Twenty-one cases were grade Ⅰ and 36 cases were grade Ⅱ according to Kellgren-Lawrence classification. Flexion contracture of knee joint ranged from 0° to 8° with an average of 1.36° and varus deformity ranged from 5° to 10° with an average of 7.60°. Preoperative arthroscopic evaluation showed that there were 11 cases with grade Ⅰ, 42 cases with gradeⅡ, and 4 cases with grade Ⅲ according to the international cartilage repair classification system (ICRS). Lysholm score, American Hospital for Special Surgery (HSS) score, and International Knee Documentation Committee (IKDC) score were used to evaluate knee function before operation, at 3 months, at 1 year after operation, and at last follow-up. Visual analogue scale (VAS) score was used to evaluate pain. The mechanical medial proximal tibial angle (mMPTA) and femoral tibial angle (FTA) were measured before operation and at last follow-up. When the internal fixator was removed, the knee arthroscopy was performed again to explore the cartilage repair condition, and the regeneration level and maturity level were selected for cartilage grading evaluation.

Results: All patients' incisions healed by first intention after operation, and no incision infection or skin necrosis occurred. After operation, the knee joint function of the patients was significantly improved, the pain symptoms were relieved, and the force line measurement reached the target set before operation. The VAS score, Lysholm score, HSS score, and IKDC score were significantly improved at 3 months, 1 year after operation, and at last follow-up when compared with those before operation. They were gradually improved with the time and there were significant differences between time points ( <0.05). mMPTA and FTA were significantly improved at last follow-up when compared with those before operation ( <0.05). When the internal fixator was removed, the arthroscopic re-assessment found that the cartilage regeneration was classified into 10 cases of grade Ⅰ and 47 cases of grade Ⅱ; 18 cases of immature cartilage regeneration and 29 cases of mature cartilage regeneration were found in the knee joints of grade Ⅱ cartilage regeneration. There was no significant difference in the cartilage regeneration grade between different ICRS gradings ( =0.176, =0.916), and the difference in maturity grading was significant ( =10.500, =0.005).

Conclusion: Arthroscopy combined with HTO for the treatment of knee medial compartment osteoarthritis can effectively improve the symptoms and function of the knee joint, and can promote the regeneration of articular cartilage.
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http://dx.doi.org/10.7507/1002-1892.202101073DOI Listing
June 2021

Dye-Sensitized Rare Earth-Doped Nanoparticles with Boosted NIR-IIb Emission for Dynamic Imaging of Vascular Network-Related Disorders.

ACS Appl Mater Interfaces 2021 Jun 16. Epub 2021 Jun 16.

College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.

Real-time dynamic vascular network imaging can provide accurate hemodynamic and anatomical information, facilitating the diagnosis of blood circulatory system-related diseases and achieving precise evaluation of therapeutic effects. luminescence imaging in the NIR-IIb biological window (1500-1700 nm) has developed into a next generation of optical imaging method with significantly improved temporal-spatial resolution and penetration depth. Unfortunately, an imaging contrast agent capable of emitting NIR-IIb luminescence with sufficient brightness in this region is lacking. Herein, we designed and proposed a type of dye-sensitized rare earth-doped nanoparticle ([email protected]) with obviously boosted NIR-IIb emission and high biocompatibility, which can be used to realize the real-time NIR-IIb luminescence imaging with high temporal-spatial resolution and contrast. The dye sensitization process provides a 40-fold enhanced brightness of the NIR-IIb emission at 1525 nm of Er. Consequently, the [email protected] was not only able to depict a vascular network but also applicable in noninvasively monitoring the dynamic vascular processes and changes in the vascular anatomy of two blood circulatory system-related disorders, including hindlimbs ischemia and atherosclerosis. Our research provides a powerful tool for evaluating vascular network-related dysfunction and physiological processes.
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http://dx.doi.org/10.1021/acsami.1c04612DOI Listing
June 2021

Connexin32 activates necroptosis through Src-mediated inhibition of caspase 8 in hepatocellular carcinoma.

Cancer Sci 2021 May 29. Epub 2021 May 29.

Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.

Necroptosis is an alternative form of programmed cell death that generally occurs under apoptosis-deficient conditions. Our previous work showed that connexin32 (Cx32) promotes the malignant progress of hepatocellular carcinoma (HCC) by enhancing the ability of resisting apoptosis in vivo and in vitro. Whether triggering necroptosis is a promising strategy to eliminate the apoptosis-resistant HCC cells with high Cx32 expression remains unknown. In this study, we found that Cx32 expression was positively correlated with the expression of necroptosis protein biomarkers in human HCC specimens, cell lines, and a xenograft model. Treatment with shikonin, a well-used necroptosis inducer, markedly caused necroptosis in HCC cells. Interestingly, overexpressed Cx32 exacerbated shikonin-induced necroptosis, but downregulation of Cx32 alleviated necroptosis in vitro and in vivo. Mechanistically, Cx32 was found to bind to Src and promote Src-mediated caspase 8 phosphorylation and inactivation, which ultimately reduced the activated caspase 8-mediated proteolysis of receptor-interacting serine-threonine protein kinase 1/3, the key molecule for necroptosis activation. In conclusion, we showed that Cx32 contributed to the activation of necroptosis in HCC cells through binding to Src and then mediating the inactivation of caspase 8. The present study suggested that necroptosis inducers could be more favorable than apoptosis inducers to eliminate HCC cells with high expression of Cx32.
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http://dx.doi.org/10.1111/cas.14994DOI Listing
May 2021

Graves' disease with anti-GQ1b antibody syndrome: a rare case report.

BMC Neurol 2021 May 28;21(1):212. Epub 2021 May 28.

Department of Neurology, the Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.

Background: Graves' disease and anti-GQ1b antibody syndrome are both autoimmune diseases, and there have been few reports on whether there is a correlation between the two. In this study, we present the case of a woman who was diagnosed with Graves' disease and anti-GQ1b antibody syndrome in succession.

Case Presentation: The chief complaints of this patient were limb weakness and blurred vision. Graves' disease was diagnosed by examination of thyroid function and thyroid autoantibodies, but the clinical symptoms were not relieved after antihyperthyroidism treatment. Finally, it was found that Graves' disease was complicated by anti-GQ1b antibody syndrome, and the symptoms were relieved after treatment with glucocorticoids and intravenous immunoglobulin. We also explored the possible mechanism of these diseases through a literature review.

Conclusions: We report a rare case of the cooccurrence of Graves' disease and anti-GQ1b antibody syndrome. Immune dysregulation might be the pathogenesis of the association, but there is no precise supporting evidence, and more research is needed.
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http://dx.doi.org/10.1186/s12883-021-02245-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161606PMC
May 2021

Design, synthesis and biological evaluation of 3, 4-disubstituted-imidazolidine-2, 5-dione derivatives as HDAC6 selective inhibitors.

Eur J Med Chem 2021 Oct 7;221:113526. Epub 2021 May 7.

Department of Medicinal Chemistry and Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44, West Wenhua Road, 250012, Jinan, Shandong, PR China. Electronic address:

HDAC6 isoform selective inhibitors can be pursued as an alternative to pan-HDACs inhibitors due to their therapeutic effect and low toxicity. Efforts of the structure optimization of our previous compound 10c (IC = 4.4 nM) resulted in a new series of 3, 4-disubstituted-imidazolidine-2, 5-dione based HDAC6 inhibitors with better HDAC6 inhibitory activities and improved selectivities. The most potent compound 71 exhibited a low nanomolar HDAC6 inhibitory activity (IC = 2.1 nM) and showed 5545-fold, 5864-fold as well as 1638-fold selectivity relative to HDAC1, HDAC2 and HDAC8, respectively. Western blot analysis further confirmed that compound 71 selectively increased the acetylation level of α-tubulin without affecting histone H3. Moreover, compound 71 also possesses good properties in term of caspase-3 activation, apoptosis induction, anti-proliferative activity, cytotoxicity and plasma stability. Therefore, compound 71 can be applied in cancer therapy or used as a lead compound to develop more potent HDAC6 selective inhibitor.
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http://dx.doi.org/10.1016/j.ejmech.2021.113526DOI Listing
October 2021

Carbon nanoparticle suspension could help get a more accurate nodal staging for patient with rectal cancer.

Sci Rep 2021 May 11;11(1):9933. Epub 2021 May 11.

Gastrointestinal Center, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, Jiangsu, People's Republic of China.

This study aimed to evaluate whether carbon nanoparticles could improve the accuracy of nodal staging in colorectal cancer (CRC). We performed a randomized controlled trial with CRC at the department of general surgery, the affiliated hospital of Nanjing University Medical School. A total of 160 patients were recruited in this research and 132 patients were included in the safety analyses. Among these patients, 72 cases were classified into control group and 60 cases into study group. The mean number of lymph nodes harvested from patients in study group was 19.3 ± 6.7 (range from 4 to 38), which was higher than that in control group (15.1 ± 5.7 (range from 3 to 29)) (p < 0.001). The mean number of positive lymph nodes got from patients in study group was 1.7 ± 3.5 (range from 0 to 22), which was also higher than that in control group (0.7 ± 1.4 (range from 0 to 7)) (p = 0.045). In study group, there were 30 patients (50%) proved to be N0, and remaining 30 patients (50%) were N1 or N2. However, 50 patients (69.4%) were N0 and 22 patients (30.6%) were N1 or N2 in control group. The rate of N0 in control group was significantly higher than that in study group (p = 0.023). Injecting carbon nanoparticle suspension could get a more accurate nodal staging to receive enough chemoradiotherapy, improving prognosis. Besides, injecting carbon nanoparticles suspension at four points 5 cm, 10 cm, 15 cm and 20 cm away from the anus by "sandwich" method was a new try.Trial registration: This study was registered with ClinicalTrials.gov, number ChiCTR1900025127 on 12/8/2019.
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http://dx.doi.org/10.1038/s41598-021-89541-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113261PMC
May 2021

Sulfated glycosaminoglycans and low-density lipoprotein receptor mediate the cellular entry of Clostridium novyi alpha-toxin.

Cell Res 2021 May 10. Epub 2021 May 10.

Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.

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http://dx.doi.org/10.1038/s41422-021-00510-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107810PMC
May 2021

Lipoprotein-Associated Phospholipase A2 Is a Risk Factor for Patients With Parkinson's Disease.

Front Neurosci 2021 20;15:633022. Epub 2021 Apr 20.

Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: To explore the association between lipoprotein-related phospholipase A2 (Lp-PLA2) and the risk of Parkinson's disease (PD).

Methods: A case-control study involving 58 hospitalized PD patients and 60 healthy controls was carried out. Serum Lp-PLA2 level was detected. According to the disease course and severity, PD patients were subdivided to analyze the clinical value of Lp-PLA2. Relationship between Lp-PLA2 and PD risk was analyzed by logistic regression. Diagnostic value of Lp-PLA2 in PD patients was investigated using receiver's operator characteristic curves.

Results: Lp-PLA2 level was significantly higher in the PD patients compared with the controls, and was significantly and positively correlated with the Hoehn-Yahr (H&Y) stage. The serum Lp-PLA2 level and H&Y stage of PD patients with a longer disease course were significantly higher than those with a shorter disease course. PD patients with milder conditions had significantly lower serum Lp-PLA2 levels than patients with severe conditions. Multivariable logistic regression analysis indicated higher Lp-PLA2 level was an independent risk factor of PD patients. Moreover, the area under the curve for Lp-PLA2 was 0.703, which was between those of homocysteine and serum amylase A.

Conclusion: To our knowledge, this is the first study to show that increased level of Lp-PLA2 is associated with the risk of PD. Lp-PLA2 may be used for early detection of PD, and provides an effective intervention target for clinical treatment of PD.
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http://dx.doi.org/10.3389/fnins.2021.633022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093434PMC
April 2021

Astragalin Inhibits the Proliferation and Migration of Human Colon Cancer HCT116 Cells by Regulating the NF-κB Signaling Pathway.

Front Pharmacol 2021 19;12:639256. Epub 2021 Apr 19.

Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, China.

Astragalin is a flavonoid found in a variety of natural plants. It has anti-inflammatory, anti-oxidant effects and has inhibited effects against several malignant tumor cell types. However, its effects on colon cancer and the molecular mechanisms have remained to be elucidated. In this study, we evaluated the inhibitory effect of astragalin on proliferation and migration of human colon cancer HCT116 cells and . Furthermore, we elucidated the mechanism of these effects. The results showed that astragalin significantly inhibited the proliferation and diffusion of HCT116 cells by induced apoptosis (by modulation of Bax, Bcl-2, P53, caspase-3, caspase 6, caspase 7, caspase 8, caspase 9 protein express) and cell cycle arrest (by modulation of Cyclin D1, Cyclin E, P21, P27, CDK2, CDK4 protein express). Moreover, astragalin suppressed HCT116 cell migration by inhibiting the expression of matrix metalloproteinases (MMP-2, MMP-9). In addition, astragalin significantly downregulated the expression of key proteins in the NF-κB signaling pathway and inhibited the transcriptional activity of NF-κB P65 stimulated with inflammatory cytokines TNF-α, thereby inhibiting the growth of colon cancer cells . Our further investigations unveiled astragalin gavage significantly reduced the proliferation of colon cancer xenograft in nude mice, experiments showed that tumor growth was related to decreased expression of apoptotic proteins in tumor tissues and decreased activity of the NF-κB signaling pathway. In summary, our results indicated that astragalin inhibits the proliferation and growth of colon cancer cells and via the NF-κB pathway. Therefore, astragalin maybe become a potential plant-derived antitumor drug for colon cancer.
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http://dx.doi.org/10.3389/fphar.2021.639256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091521PMC
April 2021

Trileaflet Aortic Valve Reconstruction Using Bovine Pericardium.

Heart Lung Circ 2021 Apr 30. Epub 2021 Apr 30.

Department of Cardiac Surgery, Asia Heart Hospital, Wuhan, P.R. China; Affiliated Wuhan University of Science and Technology, Wuhan, P.R. China. Electronic address:

Aim: For several years now, trileaflet aortic valve reconstruction has been performed in patients with various aortic valve diseases. This study aimed to explore the feasibility and durability of trileaflet aortic valve reconstruction with bovine pericardium.

Method: Trileaflet aortic valve reconstruction with bovine pericardium was performed in 519 patients with various aortic valve diseases from April 2008 to December 2019. The results for all 519 patients were reviewed retrospectively. Mean age was 48.04±19.08 years (range, 13-80 years), and 40 patients were younger than 18 years of age. Thirty-four (34) patients had aortic stenosis, 344 patients had aortic regurgitation, and 141 patients had both aortic stenosis and regurgitation. One hundred and fifty-four (154) patients had bicuspid aortic valves, and three patients had quadricuspid aortic valves. The size of the pericardial leaflets was designed to be individually proportional to the size of the aortic root.

Results: Mean length of follow-up was 41.97±22.68 years (range, 1-127 months). In total, 448 patients were reviewed, and the follow-up rate was 86.4%. All procedures were successful without conversion to prosthetic valve replacement during the procedure. Six (6) patients died after the procedure (in-hospital mortality, 1.2%). All-cause mortality occurred in 11 patients during the follow-up period. The 10-year cumulative survival rate was 94.7%±2.9%. Redo aortic valve surgery was performed in 11 patients after the procedure. The actuarial freedom from redo aortic valve surgery after the procedure was 88.2%±5.2%. At the end of the follow-up, the mean peak was 29.1±9.6 mmHg and mean gradient was 15.6±6.2 mmHg.

Conclusions: The mid- and long-term outcomes in patients with various aortic valve diseases undergoing trileaflet aortic valve reconstruction with bovine pericardium are encouraging. The engineered pericardial leaflets should be individually proportional to the size of the aortic root.
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http://dx.doi.org/10.1016/j.hlc.2021.03.278DOI Listing
April 2021

Postoperative Infection of Male Posterior Urethral Stenosis with Pelvic Fracture: A Retrospective Study from a Chinese Tertiary Teferral Center.

Urology 2021 Apr 30. Epub 2021 Apr 30.

Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital; Shanghai Oriental Institute for Urologic Reconstruction, Shanghai, 200233, China. Electronic address:

Objectives: To investigate the risk factors for postoperative infection, including systemic inflammatory response syndrome, sepsis and surgical site infection, after posterior urethral anastomosis for the treatment of male posterior urethral stenosis with pelvic fractures.

Methods: We retrospectively analyzed data from patients who underwent transperineal end-to-end anastomotic urethroplasty between January 2016 and December 2018. Descriptive statistics were used to analyze patient characteristics and perioperative features. Univariate analysis was performed to identify prognostic factors associated with postoperative infection. Multivariate analysis was used to identify independent risk factors for postoperative infection.

Results: Among 261 patients included in the analysis, 16.48% had SIRS, 3.83% had sepsis, and 8.05% had SSI. The primary results suggested that penile septum separation, inferior pubic resection, operating duration, preoperative urine culture result, preoperative waiting time, urethral stenosis length, and draining method were significant predictors of postoperative infections. Multivariate analysis revealed that more complex surgical procedures, operating duration and positive urine culture results were independent risk factors for SIRS and preoperative positive urine culture result was an independent risk factor for sepsis and SSI.

Conclusions: Positive preoperative urine culture was the main risk factor for postoperative infections. More complex surgical procedures, such as penile septum separation and inferior pubic resection, and longer operating duration were more likely to be associated with postoperative SIRS.
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http://dx.doi.org/10.1016/j.urology.2021.04.021DOI Listing
April 2021

Pancancer Analysis of Neurovascular-Related NRP Family Genes as Potential Prognostic Biomarkers of Bladder Urothelial Carcinoma.

Biomed Res Int 2021 15;2021:5546612. Epub 2021 Apr 15.

Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Background: Neurovascular-related genes have been implicated in the development of cancer. Studies have shown that a high expression of neuropilins (NRPs) promotes tumourigenesis and tumour malignancy.

Method: A multidimensional bioinformatics analysis was performed to examine the relationship between NRP genes and prognostic and pathological features, tumour mutational burden (TMB), microsatellite instability (MSI), and immunological features based on public databases and find the potential prognostic value of NRPs in pancancer.

Results: Survival analysis revealed that a low NRP1 expression in adrenocortical carcinoma (ACC), cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), low-grade glioma (LGG), and stomach adenocarcinoma (STAD) was associated with poor prognosis. A high NRP2 expression in bladder urothelial carcinoma (BLCA), kidney renal papillary cell carcinoma (KIRP), and mesothelioma (MESO) was associated with poor prognosis. Moreover, NRP1 and NRP2 were associated with TMB and MSI. Subsequent analyses showed that NRP1 and NRP2 were correlated with immune infiltration and immune checkpoints. Genome-wide association analysis revealed that the NRP1 expression was strongly associated with kidney renal clear cell carcinoma (KIRC), whereas the NRP2 expression was closely associated with BLCA. Ultimately, NRP2 was found to be involved in the development of BLCA.

Conclusions: Neurovascular-related NRP family genes are significantly correlated with cancer prognosis, TME, and immune infiltration, particularly in BLCA.
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http://dx.doi.org/10.1155/2021/5546612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062179PMC
June 2021

Application of Parameter Optimized Variational Mode Decomposition Method in Fault Feature Extraction of Rolling Bearing.

Entropy (Basel) 2021 Apr 24;23(5). Epub 2021 Apr 24.

School of Electrical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China.

The decomposition effect of variational mode decomposition (VMD) mainly depends on the choice of decomposition number K and penalty factor α. For the selection of two parameters, the empirical method and single objective optimization method are usually used, but the aforementioned methods often have limitations and cannot achieve the optimal effects. Therefore, a multi-objective multi-island genetic algorithm (MIGA) is proposed to optimize the parameters of VMD and apply it to feature extraction of bearing fault. First, the envelope entropy () can reflect the sparsity of the signal, and Renyi entropy () can reflect the energy aggregation degree of the time-frequency distribution of the signal. Therefore, and are selected as fitness functions, and the optimal solution of VMD parameters is obtained by the MIGA algorithm. Second, the improved VMD algorithm is used to decompose the bearing fault signal, and then two intrinsic mode functions (IMF) with the most fault information are selected by improved kurtosis and Holder coefficient for reconstruction. Finally, the envelope spectrum of the reconstructed signal is analyzed. The analysis of comparative experiments shows that the feature extraction method can extract bearing fault features more accurately, and the fault diagnosis model based on this method has higher accuracy.
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http://dx.doi.org/10.3390/e23050520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146961PMC
April 2021

Weakly Supervised Domain Adaptation for Aspect Extraction via Multilevel Interaction Transfer.

IEEE Trans Neural Netw Learn Syst 2021 Apr 19;PP. Epub 2021 Apr 19.

Fine-grained aspect term extraction is an essential subtask in aspect-based opinion analysis. It aims to identify the aspect terms (also known as opinion targets) of a product or service in each sentence. To learn a good aspect extraction model, an expensive annotation process is usually involved to acquire sufficient token-level labels for each domain, which is not realistic. To address this limitation, some previous works propose domain adaptation strategies to transfer knowledge from a sufficiently labeled source domain to unlabeled target domains. However, due to both the difficulty of fine-grained prediction problems and the large domain gap between different domains, the performance is still far from satisfactory. In this work, we conduct a pioneer study on leveraging sentence-level aspect category labels that can be usually available in commercial services, such as review sites or social media to promote token-level transfer for extraction purpose. Specifically, the aspect category information can be used to construct pivot knowledge for transfer with the assumption that the interactions between the sentence-level aspect category and the token-level aspect terms are invariant across domains. To this end, we propose a novel multilevel reconstruction mechanism that aligns both the fine- and coarse-grained information in multiple levels of abstractions. Comprehensive experiments over several benchmark data sets clearly demonstrate that our approach can fully utilize the sentence-level aspect category labels to improve cross-domain aspect term extraction with a large performance gain.
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http://dx.doi.org/10.1109/TNNLS.2021.3071474DOI Listing
April 2021

Newly graduated nurses' perceptions of work environment: A cross-sectional study in China.

Int J Nurs Pract 2021 Apr 10:e12929. Epub 2021 Apr 10.

Nursing Department, The First Affiliated Hospital of Kunming Medical University, Kunming, China.

Aim: To assess the attitudes of newly graduated nurses in China toward their work environment and identify the factors affecting their perception.

Background: Newly graduated nurses are the primary supply to cover the nursing shortage, but their stressful transition into clinical work has contributed to high turnover for decades. Although serving as a source of stress, the work environment can also be a source of support for new nurses.

Methods: A total of 2361 new nurses at 31 tertiary hospitals in 20 provinces were surveyed from June to November 2018, using the Chinese version of the Practice Environment Scale-Nursing Work Index. Basic information and their perception about work environment were collected.

Results: New nurses rated their work environment with an overall score of 84.67 (12.85). Multiple linear regression identified that education level, monthly income, hospital type and location, participation (or not) in standardized training programmes and having (or not) a mentor/preceptor were factors significantly associated with their perception of the work environment.

Conclusion: The perception of newly graduated nurses in China was mainly positive overall. Nursing managers may take full advantage of factors affecting the perception of work environment to support newly graduated nurses better, to help their retention and transition.

Summary Statement: What is already known about the topic? Newly graduated nurses experience transition shock at the beginning of their work. Supportive work environments can help successful transition. What this paper adds? Newly graduated nurses are not fully supported in their work environment. Staffing and resource inadequacy were the poorest environmental factors. Residency programmes had the most impact on the perceptions of newly graduated nurses about their work environment.

Implications For Nursing Practice: These results can guide nursing managers to support the newly graduated nurses better in their transition process, thus helping retention of nursing human resource.
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http://dx.doi.org/10.1111/ijn.12929DOI Listing
April 2021

and antibacterial performance of Zr & O PIII magnesium alloys with high concentration of oxygen vacancies.

Bioact Mater 2021 Oct 9;6(10):3049-3061. Epub 2021 Mar 9.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

The effects of dual Zr and O plasma immersion ion implantation (Zr & O PIII) on antibacterial properties of ZK60 Mg alloys are systematically investigated. The results show that a hydrophobic, smooth, and ZrO-containing graded film is formed. Electrochemical assessment shows that the corrosion rate of the plasma-treated Mg alloy decreases and the decreased degradation rate is attributed to the protection rendered by the surface oxide. and antibacterial tests reveal Zr & O PIII ZK60 presents higher antibacterial rate compared to Zr PIII ZK60 and untreated control. The hydrophobic and smooth surface suppresses bacterial adhesion. High concentration of oxygen vacancies in the surface films are determined by X-ray photoelectron spectroscopy (XPS), UV-vis diffuse reflectance spectra (UV-vis DRS) and electron paramagnetic resonance (EPR) and involved in the production of reactive oxygen species (ROS). The higher level of ROS expression inhibits biofilm formation by down-regulating the expression of genes but up-regulating the expression of gene. In addition, Zr & O PIII improves cell viability and initial cell adhesion confirming good cytocompatibility. Dual Zr & O PIII is a simple and practical means to expedite clinical acceptance of biodegradable magnesium alloys.
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http://dx.doi.org/10.1016/j.bioactmat.2021.02.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960947PMC
October 2021

Two-sewing-ring technique for a self-assembled aortic valve conduit to the annulus.

Ann Thorac Surg 2021 Mar 23. Epub 2021 Mar 23.

Wuhan Asia Heart Hospital, Jinghan Road 753, Wuhan, China. Electronic address:

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http://dx.doi.org/10.1016/j.athoracsur.2020.11.094DOI Listing
March 2021

Neoadjuvant Chemoradiotherapy Does Not Contribute to Worse Survival in Pathological Node-Negative Rectal Cancer.

Front Oncol 2021 8;11:649313. Epub 2021 Mar 8.

Department of General Surgery, Jiangdu People' s Hospital Affiliated to Yangzhou University Medical School, Yangzhou, China.

The prognostic significance of ypN0 rectal cancer with comparison to pN0 disease still remains poorly defined. This study aimed to compare the prognosis of ypN0 and pN0 rectal cancer. Eligible patients were identified from the SEER18 registries research database (the latest data up to date was on April 15, 2019). Propensity score (PS) matching was usually performed to reduce the imbalance and potential confounding that were introduced by inherent differences between the groups. The cause-specific survival (CSS) was analyzed to evaluate the prognostic prediction of ypN0 and pN0 groups using the Kaplan-Meier method with the log-rank test. Cox proportional hazard model was also used to identify independent prognostic variables. In total, 26,832 patients diagnosed with pN0 or ypN0 rectal cancer were confirmed as the final cohort, including 7,237 (27.0%) patients with radiation and 19,595 (73.0%) patients without radiation prior to surgery. The median follow-up time was up to 81 months. After adjusting for other prognostic factors, neoadjuvant radiotherapy was not an independent prognostic variable of CSS (HR = 1.100, 95%CI = 0.957-1.265, = 0.180, using pN0 group as the reference). ypN0 rectal cancer was strongly associated with worse pathological diagnoses compared with pN0 rectal cancer, contributing to worse oncologic outcomes. However, the receipt of neoadjuvant chemoradiotherapy was not an independent prognostic factor of worse prognosis in pathological node-negative patients. Our study could give guidance to the treatment of ypN0 rectal cancer.
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http://dx.doi.org/10.3389/fonc.2021.649313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982457PMC
March 2021

Distribution and migration characteristics of dinitrotoluene sulfonates (DNTs) in typical TNT production sites: Effects and health risk assessment.

J Environ Manage 2021 Jun 16;287:112342. Epub 2021 Mar 16.

College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China.

The production of 2,4,6-trinitrotoluene (TNT) produces a great deal of waste water, and dinitrotoluene sulfonates (DNTs) are the main pollutants in its waste. This paper presents a pilot investigation on the geochemical transformation of DNTs affected by historical wastewater spillage from a typical TNT production company in Northwest China. In the horizontal direction, DNTs diffused from the evaporation pond to the surrounding area of the site, and the concentration of DNTs in the evaporation pond surface soil exceeded 1000 mg/kg. The horizontal distribution of DNTs in the site showed a migration trend to the east and south of evaporation, which was consistent with the terrain of high northwest and low southeast of the site. Due to the high water solubility of pollutants, water flow is the main driving force for the horizontal distribution of DNTs. In the vertical direction, the concentration of pollutants gradually increased with the depth of the soil. DNTs are mainly adsorbed in the third layer (6.0-8.0 m). It can be seen that the accumulation of the 2,4-DNTs-3-SO is obviously larger than that of the 2,4-DNTs-5-SO, which may be related to the steric hindrance effect of sulfonic acid groups in the two isomers. Results showed DNTs distribution strongly linked to soil physicochemical properties and the migration of DNTs in soil exhibited obvious heterogeneity in time and space. The carcinogenic risks in surface soil (0-1.5 m) and lower soil (1.5-6.0 m, 6.0-8.0 m) are all higher than 1✕10; non-carcinogenic risk surface soil (0-1.5 m) is 4.011✕10, which is greater than 1, indicating that they may cause certain harm to the human body. Meanwhile, this study presented a pioneering investigation for the contamination and geochemical transfer of DNTs.
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http://dx.doi.org/10.1016/j.jenvman.2021.112342DOI Listing
June 2021

Serum-Derived Exosomes from Patients with Coronary Artery Disease Induce Endothelial Injury and Inflammation in Human Umbilical Vein Endothelial Cells.

Int Heart J 2021 Mar 17;62(2):396-406. Epub 2021 Mar 17.

Second School of Clinical Medicine, Southern Medical University.

Endothelial injury and inflammation have been found to be essential in the pathogenesis of coronary artery disease (CAD). Circulating exosomes are of great value as novel biomarkers for CAD. However, the role of circulating exosomes in the pathogenesis of CAD remains unclear. Thus, in this study, we aimed to examine whether circulating exosomes from CAD are involved in the endothelial injury and inflammation. The serum-derived exosomes were isolated from CAD and controls using an ExoQuick reagent, and these were then quantified by measuring the protein levels using BCA methods. The uptake of exosomes by human umbilical vein endothelial cells (HUVECs) was observed by laser scanning microscope and analyzed via flow cytometry. Then, HUVECs were treated with vehicle, exosomes from CAD (CAD-exo), and controls (ctrl-exo) in the absence and presence of vascular endothelial growth factor (VEGF). Cell viability, migration, and angiogenesis were evaluated using CCK-8 assay, scratch assay, and tube formation assay. Inflammatory factors including IL-1β, IL-6, TNF-α, ICAM-1, and VCAM-1 levels were detected via qPCR. As per our findings, no significant differences were noted in uptake of ctrl-exo and CAD-exo by HUVECs. CAD-exo suppressed cell viability in a dose-dependent manner. Compared with ctrl-exo, CAD-exo-treated HUVECs significantly suppressed migration and angiogenesis. However, CAD-exo had a stronger inhibitory effect on VEGF-induced migration and angiogenesis compared with ctrl-exo. Moreover, IL-1β, TNF-α, and ICAM-1 were determined to be significantly upregulated in HUVECs treated with CAD-exo, but IL-6 and VCAM-1 expressions were not affected. Overall, our results suggest that CAD-exo are involved in endothelial injury and inflammation, which may, in turn, cause endothelial dysfunction and potentially promote the development of CAD.
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http://dx.doi.org/10.1536/ihj.20-641DOI Listing
March 2021

Circulating Exosomal miR-96 as a Novel Biomarker for Radioresistant Non-Small-Cell Lung Cancer.

J Oncol 2021 27;2021:5893981. Epub 2021 Feb 27.

Department of Oncology, Nanjing Second Hospital, Nanjing University of Chinese Medicine, Nanjing 210003, Jiangsu, China.

Patients with non-small-cell lung cancer (NSCLC) frequently develop radioresistance, resulting in poor response to radiation and unfavourable prognosis. Early detection of radioresistance hence can guide the adjustment of treatment regimens in time. Exosomes are lipid bilayer-enclosed vesicles with sub-micrometer size that are released by various cells. Exosomes contain a tissue-specific signature wherein a variety of proteins and nucleic acids are selectively packaged. Growing evidence shows exosomes are involved in cancer pathophysiology and exosomes as the latest addition to the liquid biopsy portfolio have been used in cancer diagnosis. Compared to cell free RNA, exosomal lipid envelope can effectively protect RNA cargo against degradation. Therefore, exosomes may hold great promise for the identification of radioresistance. Here, we report six plasma exosomal miRNAs could be used to distinguish radioresistant NSCLC patients from radiosensitive NSCLC patients and to evaluate the prognosis of NSCLC. Samples were obtained from 52 NSCLC patients with or without radioresistance and 45 age-matched healthy volunteers. Exosomes in 1 ml plasma were isolated followed by extraction of small RNA. The expression levels of miRNAs were determined by quantitative real-time PCR. Potential miRNA markers were further evaluated in additional 52 NSCLC patients. We found exosomal miR-1246 and miR-96 are significantly overexpressed in NSCLC patients. Moreover, exosomal miR-96 in patients with radioresistant NSCLC is significantly higher than that of controls. Exosomal miR-96 also demonstrates a significant correlation with vascular invasion and poor overall survival. Altogether, our results indicate that exosomal miR-96 could be a non-invasive diagnostic and prognostic marker of radioresistant NSCLC.
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http://dx.doi.org/10.1155/2021/5893981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937465PMC
February 2021
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