Publications by authors named "Tao Li"

5,338 Publications

  • Page 1 of 1

Ultralow-Loading Pt/Zn Hybrid Cluster in Zeolite HZSM-5 for Efficient Dehydroaromatization.

J Am Chem Soc 2022 Jun 24. Epub 2022 Jun 24.

Dave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, Mississippi 39762, United States.

Minimizing Pt loading without sacrificing catalytic performance is critical, particularly for designing cost-efficient hydrocarbon transformation catalysts. Here, we show that ultralow-loading (0.001-0.05 wt %) Pt- and Zn-functionalized HZSM-5 catalysts, prepared through simple ion exchange and impregnation, are highly active and stable for light alkane dehydroaromatization (DHA). The specific activity of benzene, toluene, and xylene is up to 8.2 mol/g/min (or 1592 min) over the 0.001 wt % Pt-Zn/HZSM-5 catalyst during ethane DHA at 550 °C under atmospheric pressure. Additionally, such bimetallic Pt-Zn/HZSM-5 catalysts are highly stable in contrast to the monometallic Pt/HZSM-5 catalysts. The rate constant of deactivation (), according to the first-order generalized power law equation model, for the bimetallic catalysts is up to 120 times lower than that of the monometallic counterparts, depending on the Pt loading. This breakthrough is achieved through the formation of the [Pt-Zn] hybrid cluster, instead of Pt cluster-proton adducts, in the micropores of the ZSM-5 zeolite.
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http://dx.doi.org/10.1021/jacs.2c04278DOI Listing
June 2022

Sulfate Freeze-Thaw Resistance of Magnesium Potassium Phosphate Cement Mortar according to Hydration Age.

Materials (Basel) 2022 Jun 13;15(12). Epub 2022 Jun 13.

College of Civil Engineering and Architecture, Jiangsu University of Science and Technology, Zhenjiang 212000, China.

Concrete structures can be degraded by exposure to environmental stressors such as freeze-thaw cycling and salt corrosion. Magnesium potassium phosphate cement (MKPC) mortar is useful for the rapid repair of such structures but must acquire environmental resistance rapidly. In this study, the freeze-thaw resistance of MKPC mortar specimens of different hydration ages was tested in water and a 5% NaSO solution. The strength, volume deformation, and water absorption rates were compared with those of full-age MKPC mortar specimens (28 d). The phase composition and microscopic morphology of the MKPC mortar specimens before and after corrosion were observed, and the corrosion-resistance mechanism was analyzed. After 225 freeze-thaw cycles in water and sulfate solution, the strength residual rates of the early-age specimen (1 d) were higher than those of the full-age specimen (28 d). The degree of strength attenuation in the 1 d specimen was lower in the sulfate environment than in the water environment. After 225 freeze-thaw cycles, the volume expansion rates of 1 d specimens in water or sulfate were 0.487% and 0.518%, respectively, while those of 28 d specimens were 0.963% and 1.308%. The comparison shows that the 1 d specimen had significantly better deformation resistance under freeze-thaw than the 28 d specimen. After 225 freeze-thaw cycles, the water absorption rates of 1 d specimens were 1.95% and 1.64% in water and sulfate solution, respectively, while those of 28 d specimens were 2.20% and 1.83%. This indicates that freeze-thaw cycling has a greater effect on the pore structure of fully aged mortar than on early-age mortar (1 d). Therefore, MKPC mortar is suitable for the rapid repair of concrete structures in harsh environments. The results form a theoretical basis for winter emergency repair projects. They also further the understanding of the application of MKPC-based materials in extreme environments.
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http://dx.doi.org/10.3390/ma15124192DOI Listing
June 2022

Maximum Power Point Tracking Control for Non-Gaussian Wind Energy Conversion System by Using Survival Information Potential.

Entropy (Basel) 2022 Jun 11;24(6). Epub 2022 Jun 11.

School of Ationautom, Nanjing University of Information Science & Technology, Nanjing 210044, China.

In this paper, a wind energy conversion system is studied to improve the conversion efficiency and maximize power output. Firstly, a nonlinear state space model is established with respect to shaft current, turbine rotational speed and power output in the wind energy conversion system. As the wind velocity can be descried as a non-Gaussian variable on the system model, the survival information potential is adopted to measure the uncertainty of the stochastic tracking error between the actual wind turbine rotation speed and the reference one. Secondly, to minimize the stochastic tracking error, the control input is obtained by recursively optimizing the performance index function which is constructed with consideration of both survival information potential and control input constraints. To avoid those complex probability formulation, a data driven method is adopted in the process of calculating the survival information potential. Finally, a simulation example is given to illustrate the efficiency of the proposed maximum power point tracking control method. The results demonstrate that by following this method, the actual wind turbine rotation speed can track the reference speed with less time, less overshoot and higher precision, and thus the power output can still be guaranteed under the influence of non-Gaussian wind noises.
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http://dx.doi.org/10.3390/e24060818DOI Listing
June 2022

The Performance Comparison of Socioeconomic and Behavioural Factors as Predictors of Higher Blood Lead Levels of 0-6-Year-Old Chinese Children between 2004 and 2014.

Children (Basel) 2022 May 30;9(6). Epub 2022 May 30.

Child Health Big Data Research Centre, Capital Institute of Pediatrics, Beijing 100020, China.

Childhood lead exposure is a commonly known risk factor affecting children's health, and 10 governments have taken actions to reduce children's lead exposure sources. Because lab testing for children's blood lead levels (BLLs) was not popularized easily, socioeconomic and behavioural factors have been usually used as predictors of screening methods. Along with the overall decreasing trend of children's BLLs, the lead-exposure-potential-predicting ability of such factors might be limited or changed over time. Our study aims to compare the predicting ability of multiple factors, including the living environment, economic disparity and personal behaviour differences between 2004 and 2014. With potential predicting factors identified, it could provide direction in identifying individual children facing high-risk lead exposure in the unit of clinics or communities of China. The study was first conducted in 12 cities in China in 2004 and then repeated in 2014 in the same 12 cities with the same method. In total, 27,972 children aged under 7 years were included in this study. With confounding factors adjusted, the child's age, the family's socioeconomic status and the child's personal hygiene habit, especially biting toys, continued to be important predictors of higher blood lead levels among Chinese children. The sex of the child was no longer a predictor. Factors such as the father's occupational contact with lead, residence near the main road and taking traditional Chinese medicine had the potential to be new predictors.
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http://dx.doi.org/10.3390/children9060802DOI Listing
May 2022

Mycotoxin DON Accumulation in Wheat Grains Caused by Fusarium Head Blight Are Significantly Subjected to Inoculation Methods.

Toxins (Basel) 2022 Jun 15;14(6). Epub 2022 Jun 15.

Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Collaborative Innovation of Modern Crops and Food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou University, Yangzhou 225009, China.

The disease severity and mycotoxin DON content in grains caused by fusarium head blight (FHB) have been two prioritized economical traits in wheat. Reliable phenotyping is a prerequisite for genetically improving wheat resistances to these two traits. In this study, three inoculation methods: upper bilateral floret injection (UBFI), basal bilateral floret injection (BBFI), and basal rachis internode injection (BRII), were applied in a panel of 22 near-isogenic lines (NILs) contrasting in alleles. The results showed that inoculation methods had significant influence on both disease severity and mycotoxin accumulation in grains, and the relationship between them. UBFI method caused chronic FHB symptom characterized as slow progress of the pathogen downward from the inoculation site, which minimized the difference in disease severity of the NILs, but, unexpectedly, maximized the difference in DON content between them. The BBFI method usually caused an acute FHB symptom in susceptible lines characterized as premature spike death (PSD), which maximized the difference in disease severity, but minimized the difference in DON content in grains between resistant and susceptible lines. The BRII method occasionally caused acute FHB symptoms for susceptible lines and had relatively balanced characteristics of disease severity and DON content in grains. Therefore, two or more inoculation methods are recommended for precise and reliable evaluation of the overall resistance to FHB, including resistances to both disease spread within a spike and DON accumulation in grains.
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http://dx.doi.org/10.3390/toxins14060409DOI Listing
June 2022

Rapid identification of the storage duration and species of sliced boletes using near-infrared spectroscopy.

J Food Sci 2022 Jun 23. Epub 2022 Jun 23.

Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, China.

Boletes are recognized as a worldwide delicacy. Adulteration of the expired and low-value sliced boletes is a pressing problem in the supply chain of commercial sliced boletes. This study aimed at developing a rapid method to identify the storage duration and species of sliced boletes, using near-infrared (NIR) spectroscopy. In the study, 1376 fruiting bodies of wild-grown boletes were collected from 2017 to 2020 in Yunnan, containing four common species of edible boletes. A NIR spectroscopy-based strategy was proposed, that is, identify the storage duration of sliced boletes to ensure that they are within the shelf life firstly; then identify the species of sliced boletes within the shelf life to evaluate their economic value. Three supervised methods, partial least squares discriminant analysis (PLS-DA), extreme learning machine (ELM), and two-dimensional correlation spectroscopy (2DCOS) images with residual convolutional neural network (ResNet) model were applied to identify. The results showed that PLS-DA model cannot accurately identify the storage duration and species of sliced boletes, and the ELM model can identify the storage duration of boletes samples, but cannot accurately discriminate different species of samples. And ResNet model established by 2DCOS images showed superiority in classification performance, 100% accuracy was obtained for both the storage duration and species classification. Moreover, compared to traditional methods, the 2DCOS images with ResNet model was free of complicated data preprocessing. The results obtained in the present study indicated a promising way of combining 2DCOS images with ResNet methods, in tandem with NIR for the rapid identification of the storage duration and species of sliced boletes. PRACTICAL APPLICATION: In the boletes supply chain, the method can be considered as a reliable method for testing the authenticity of boletes slices. The current study can also provide a reference for quality control of other edible mushroom.
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http://dx.doi.org/10.1111/1750-3841.16220DOI Listing
June 2022

Antifibrotic effect of AD-1 on lipopolysaccharide-mediated fibroblast injury in L929 cells and bleomycin-induced pulmonary fibrosis in mice.

Food Funct 2022 Jun 23. Epub 2022 Jun 23.

Shenyang Pharmaceutical University, Shenyang 110016, China.

20()-25-methoxyl-dammarane-3β,12β,20-triol (25-OCH-PPD, AD-1) is a dammarane ginsenoside that is isolated from . The present study aimed to explore its anti-pulmonary fibrosis (PF) effect and . L929 cells were treated with 10 μg mL lipopolysaccharide (LPS) to establish a PF model and mice were administered with 3.5 mg kg bleomycin (BLM) by endotracheal intubation to establish a PF model for investigating the anti-PF effect and its potential mechanism. The results demonstrated that AD-1 reduced the injury, extracellular matrix (ECM) buildup and α-smooth muscle actin (α-SMA) protein expression levels of L929 induced by LPS. Oral administration of AD-1 downregulated the expression of interleukins (such as IL-1β, IL-6 and IL-18), increased the expression of superoxide dismutase (SOD) and glutathione (GSH), reduced the lung coefficient and the content of hydroxyproline (HYP), and mediated the Bax/Bcl-2 protein ratio and P-p53, β-catenin and SIRT3 expression in the lung tissue of mice. Furthermore, AD-1 inhibited the expression levels of TGF-β1, TIMP-1 and α-SMA and reduced inflammatory cell infiltration and collagen deposition in the lung tissue of PF mice. These results indicated that AD-1 could alleviate PF both and , and the underlying mechanism may be related to the decrease in ECM deposition and inflammation, the enhancement of antioxidant capacity, and the mediation of lung cell apoptosis and the TGF-β1/TIMP-1/α-SMA signaling pathway, which provide a theoretical basis for the rehabilitation treatment of PF.
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http://dx.doi.org/10.1039/d1fo04212bDOI Listing
June 2022

Sperm-derived RNAs improve the efficiency of somatic cell nuclear transfer (SCNT) through promoting R-loop formation.

Mol Reprod Dev 2022 Jun 23. Epub 2022 Jun 23.

Department of Animal Science, Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang, Shaanxi, China.

Mammalian sperm and oocytes are haploid cells that carry parental genetic and epigenetic information for their progeny. The cytoplasm of oocytes is also capable of reprograming somatic cells to establish totipotency through somatic cell nuclear transfer (SCNT). However, epigenetic barriers seriously counteract SCNT reprogramming. Here, we found that sperm-derived RNAs elevated chromatin accessibility of nuclear donor cells concurrent with the appearance of increased RNA amount and decreased cell proliferation, instead of activating DNA damage response. Additionally, tri-methylation of lysine 9 on histone H3 (H3K9me3) and the H3K9 methyltransferase SUV39H2 were significantly downregulated by the sperm-derived RNA treatment. Our findings thus raise a fascinating possibility that sperm RNA-induced R-loops may activate gene expression and chromatin structure, thereby promoting SCNT reprogramming.
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http://dx.doi.org/10.1002/mrd.23627DOI Listing
June 2022

Ultramicroporous Organophosphorus Polymers via Self-Accelerating P-C Coupling Reactions: Kinetic Effects on Crosslinking Environments and Porous Structures.

J Am Chem Soc 2022 Jun 23. Epub 2022 Jun 23.

School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, People's Republic of China.

Porous organic polymers (POPs) have drawn significant attention in diverse applications. However, factors affecting the heterogeneous polymerization and porosity of POPs are still not well understood. Herein, we report a new strategy to construct porous organophosphorus polymers (POPPs) with high surface areas (1283 m/g) and ultramicroporous structures (0.67 nm). The strategy harnesses an efficient transition-metal-catalyzed phosphorus-carbon (P-C) coupling reaction at the trigonal pyramidal P-center, which is distinct from the typical carbon-carbon coupling reaction utilized in the synthesis of POPs. As the first kinetic study on the coupling reaction of POPs, we uncovered a self-accelerating reaction characteristic, which is controlled by the choice of bases and catalysts. The self-accelerating characteristic of the P-C coupling reaction is beneficial for the high surface area and uniform ultramicroporosity of POPPs. The direct crosslinking of the P-centers allows P solid-state (ss)NMR experiments to unambiguously reveal the crosslinking environments of POPPs. Leveraging on the kinetic studies and P ssNMR studies, we were able to reveal the kinetic effects of the P-C coupling reaction on both the crosslinking environments and the porous structures of POPPs. Furthermore, our studies show that the CO uptake capacity of POPPs is highly dependent on their porous structures. Overall, our studies paves the way to design new POPs with better controlled chemical and ultramicroporous structures, which have potential applications for CO capture and separation.
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http://dx.doi.org/10.1021/jacs.2c03759DOI Listing
June 2022

Research Trends on Pulmonary Rehabilitation: A Bibliometric Analysis From 2011 to 2020.

Authors:
Tao Li Jia Chen

Front Med (Lausanne) 2022 6;9:887793. Epub 2022 Jun 6.

Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China.

Background And Objective: A mounting body of evidence suggests that lung function may deteriorate over time with the development of chronic lung diseases (CRDs). Pulmonary rehabilitation has been proved to improve exercise capacity and quality of life in individuals with CRDs. However, PR remains grossly underutilized all around the world. This study aimed to analyze the research trends on PR over the past 10 years.

Methods: The publications related to pulmonary rehabilitation in the Web of Science Core Collection (WoSCC) from 2011 to 2020 were searched. VOSviewer (1.6.15) and CiteSpace Software (5.5.R2) were used to analyze authors and co-cited authors, countries and institutions, journals and co-cited journals, co-cited references, and keywords.

Results: A total of 4,521 publications were retrieved between 2011 and 2020, and the number of annual publications on pulmonary rehabilitation has shown an overall upward trend in the past decade. The USA was the most productive country, the University of Toronto from Canada was both the first in publications and citations. Spruit MA was both the most productive author and the one with the highest number of co-citations. The first productive journal was the International Journal of Chronic Obstructive Pulmonary Disease, while the first co-cited journal was the American Journal of Respiratory and Critical Care Medicine. The hot keywords were grouped into three clusters, while "Asthma" and "Respiratory society statement" were determined as the frontier topics.

Conclusions: The present study successfully revealed the research status and development trends of pulmonary rehabilitation from 2011 to 2020 by using bibliometric analysis, which may help researchers explore and discover new research directions in the future.
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http://dx.doi.org/10.3389/fmed.2022.887793DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207922PMC
June 2022

Identification of novel proteins for lacunar stroke by integrating genome-wide association data and human brain proteomes.

BMC Med 2022 Jun 23;20(1):211. Epub 2022 Jun 23.

Department of Neurobiology, Affiliated Mental Health Center & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Background: Previous genome-wide association studies (GWAS) have identified numerous risk genes for lacunar stroke, but it is challenging to decipher how they confer risk for the disease. We employed an integrative analytical pipeline to efficiently transform genetic associations to identify novel proteins for lacunar stroke.

Methods: We systematically integrated lacunar stroke genome-wide association study (GWAS) (N=7338) with human brain proteomes (N=376) to perform proteome-wide association studies (PWAS), Mendelian randomization (MR), and Bayesian colocalization. We also used an independent human brain proteomic dataset (N=152) to annotate the new genes.

Results: We found that the protein abundance of seven genes (ICA1L, CAND2, ALDH2, MADD, MRVI1, CSPG4, and PTPN11) in the brain was associated with lacunar stroke. These seven genes were mainly expressed on the surface of glutamatergic neurons, GABAergic neurons, and astrocytes. Three genes (ICA1L, CAND2, ALDH2) were causal in lacunar stroke (P < 0.05/proteins identified for PWAS; posterior probability of hypothesis 4 ≥ 75 % for Bayesian colocalization), and they were linked with lacunar stroke in confirmatory PWAS and independent MR. We also found that ICA1L is related to lacunar stroke at the brain transcriptome level.

Conclusions: Our present proteomic findings have identified ICA1L, CAND2, and ALDH2 as compelling genes that may give key hints for future functional research and possible therapeutic targets for lacunar stroke.
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http://dx.doi.org/10.1186/s12916-022-02408-yDOI Listing
June 2022

Exposure to Per- and Polyfluoroalkyl Substances and Mortality in U.S. Adults: A Population-Based Cohort Study.

Environ Health Perspect 2022 Jun 22;130(6):67007. Epub 2022 Jun 22.

Laboratory of Mitochondria and Metabolism, Department of Anesthesiology, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, China.

Background: Per- and polyfluoroalkyl substances (PFAS) are widespread environmental contaminants associated with diseases such as cancer and dyslipidemia. However, few studies have investigated the association between PFAS mixture exposure and mortality in general populations.

Objectives: This study aimed to explore the association between PFAS mixture, perfluorooctanoic acid (PFOA), and perfluorooctane sulfonic acid (PFOS) and mortality in U.S. adults by a nationally representative cohort.

Methods: Adults of age who were enrolled in the National Health and Nutrition Examination Survey (NHANES) (1999-2014) were included in our study. Baseline serum concentrations of seven PFAS were measured and individuals were followed up to 31 December 2015. Hazard ratios (HRs) and confidence intervals (CIs) were estimated using Cox proportional hazards models. Association between PFAS mixture exposure and mortality was analyzed using the -means method by clustering PFAS mixtures into subgroups. Association between PFOA/PFOS exposure and mortality was subsequently analyzed in both continuous and categorical models.

Results: During the follow-up period, 1,251 participants died. In the mixture analysis, the -means algorithm clustered participants into low-, medium-, and high-exposure groups. Compared with the low-exposure group, participants in the high-exposure group showed significantly higher risks for all-cause mortality (; 95% CI: 1.07, 1.80), heart disease mortality (; 95% CI: 1.05, 2.51), and cancer mortality (; 95% CI: 1.08, 2.84). In single PFAS analysis, PFOS was found to be positively associated with all-cause mortality (third vs. first tertile ; 95% CI: 1.22, 2.07), heart disease mortality (third vs. first tertile ; 95% CI: 1.09, 2.57), and cancer mortality (third vs. first tertile ; 95% CI: 1.10, 2.83), whereas PFOA exposure had no significant association with mortality. Assuming the observed association is causal, the number of deaths associated with PFOS exposure ( vs. ) was (95% CI: 176,000, 588,000) annually between 1999 and 2015, and it decreased to 69,000 (95% CI: 28,000, 119,000) annually between 2015 and 2018. The association between PFOS and mortality was stronger among women and people without diabetes.

Discussion: We observed a positive association between PFAS mixture exposure and mortality among U.S. adults. Limitations of this study include the potential for unmeasured confounding, selection bias, a relatively small number of deaths, and only measuring PFAS at one point in time. Further studies with serial measures of PFAS concentrations and longer follow-ups are necessary to elucidate the association between PFAS and mortality from specific causes. https://doi.org/10.1289/EHP10393.
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http://dx.doi.org/10.1289/EHP10393DOI Listing
June 2022

Direct Spectroscopic Evidence for Charge-Assisted Hydrogen-Bond Formation between Ionizable Organic Chemicals and Carbonaceous Materials.

Environ Sci Technol 2022 Jun 21. Epub 2022 Jun 21.

Stockbridge School of Agriculture, University of Massachusetts, Amherst, Massachusetts 01003, United States.

The direct evidence for the formation of charge-assisted hydrogen bond (CAHB) between the charged groups of ionizable organic chemicals (IOCs) and carbonaceous materials with similar proton affinity remains elusive. We therefore selected three pharmaceutical contaminants (PCs) as representative IOCs to provide the direct evidence of CAHB formation between IOCs and functionalized carbon nanotubes (CNTs) and its intensity/contribution to PC sorption on CNTs by NMR, FTIR, and DFT analyses. Sorption of PCs on functionalized CNTs resulted in the FTIR characteristic peak that appeared at a higher frequency (3787 cm) and the H NMR characteristic peak that emerged at an extremely low-field region (<18.0 ppm), which can be used as the direct spectroscopic evidence for CAHB formation. Both homonuclear CAHB (HM-CAHB, e.g., [O-H···O]) and heteronuclear CAHB (HT-CAHB, e.g., [N-H···O]/[O-H···N]) exhibited a much higher sorption energy (|| ≥ 56.24 kJ/mol) than ordinary hydrogen bond (OHB, || ≤ 6.136 kJ/mol), leading to a greater sorption contribution (HM-/HT-CAHB ≥ 42.3%, OHB ≤ 36.5%) and irreversibility (hysteresis index: HM-/HT-CAHB ≥ 1.69, OHB ≤ 0.43) of PCs on CNTs. This work presents the direct evidence for CAHB formation between IOCs and CNTs, which is significant for understanding and predicting the environmental fate and risk of IOCs, thus providing new insights for controlling their pollution using specifically designed carbonaceous materials.
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http://dx.doi.org/10.1021/acs.est.2c00417DOI Listing
June 2022

A novel approach GRNTSTE to reconstruct gene regulatory interactions applied to a case study for rat pineal rhythm gene.

Sci Rep 2022 Jun 17;12(1):10227. Epub 2022 Jun 17.

College of Computer and Information Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.

Accurate inference and prediction of gene regulatory network are very important for understanding dynamic cellular processes. The large-scale time series genomics data are helpful to reveal the molecular dynamics and dynamic biological processes of complex biological systems. Firstly, we collected the time series data of the rat pineal gland tissue in the natural state according to a fixed sampling rate, and performed whole-genome sequencing. The large-scale time-series sequencing data set of rat pineal gland was constructed, which includes 480 time points, the time interval between adjacent time points is 3 min, and the sampling period is 24 h. Then, we proposed a new method of constructing gene expression regulatory network, named the gene regulatory network based on time series data and entropy transfer (GRNTSTE) method. The method is based on transfer entropy and large-scale time-series gene expression data to infer the causal regulatory relationship between genes in a data-driven mode. The comparative experiments prove that GRNTSTE has better performance than dynamical gene network inference with ensemble of trees (dynGENIE3) and SCRIBE, and has similar performance to TENET. Meanwhile, we proved that the performance of GRNTSTE is slightly lower than that of SINCERITIES method and better than other gene regulatory network construction methods in BEELINE framework, which is based on the BEELINE data set. Finally, the rat pineal rhythm gene expression regulatory network was constructed by us based on the GRNTSTE method, which provides an important reference for the study of the pineal rhythm mechanism, and is of great significance to the study of the pineal rhythm mechanism.
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http://dx.doi.org/10.1038/s41598-022-14903-6DOI Listing
June 2022

Telomere G-triplex lights up Thioflavin T for RNA detection: new wine in an old bottle.

Anal Bioanal Chem 2022 Jun 21. Epub 2022 Jun 21.

State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology - Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences, Wuhan, 430071, China.

Few reports are found working on the features and functions of the human telomere G-triplex (ht-G3) though the telomere G-quadruplex has been intensely studied and widely implemented to develop various biosensors. We herein report that ht-G3 lights up Thioflavin T (ThT) and establish a sensitive biosensing platform for RNA detection by introducing a target recycling strategy. An optimal condition was selected out for ht-G3 to promote ThT to generate a strong fluorescence. Accordingly, an ht-G3-based molecular beacon was successfully designed against the corresponding RNA sequence of the SARS-CoV-2 N-gene. The sensitivity for the non-amplified RNA target achieves 0.01 nM, improved 100 times over the conventional ThT-based method. We believe this ht-G3/ThT-based label-free strategy could be widely applied for RNA detection.
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http://dx.doi.org/10.1007/s00216-022-04180-7DOI Listing
June 2022

Bio-electrocatalytic degradation of tetracycline by stainless-steel mesh based molybdenum carbide electrode.

Environ Sci Pollut Res Int 2022 Jun 20. Epub 2022 Jun 20.

School of Civil Engineering, Southeast University, Dong Nan Da Xue Road 2, Nanjing, 211189, People's Republic of China.

In order to treat antibiotic wastewater with high efficiency and low energy consumption, this study proposed the coupling of electrocatalytic degradation and biodegradation, and explored a new modified electrocatalytic material in the coupling system. The stainless-steel mesh based molybdenum carbide (SS-MoC) was prepared by a low-cost impregnation method and showed superior electrocatalytic degradation ability for tetracycline (TC) when used as the anode in the electrocatalytic system. The degradation rate of TC with SS-MoC anode was 17 times higher than that of stainless-steel (SS) anode, and TC removal efficiency was 77% higher than that of SS anode. The electrocatalytic system prior to the biological reactor was proven to be the optimal coupling method. The external coupling system achieved a significantly higher TC removal (87.0%) than that of the internal coupling system (65.3%) and SS-MoC showed an excellent repeatable and stable performance. The fewer and smaller molecular weight intermediates products were observed in bio-electrocatalytic system, especially in the external coupling system. Alpha diversity analysis further confirmed that bio-electrocatalytic system increased the diversity of the microbial community. The stainless-steel mesh based molybdenum carbide (SS-MoC), which was prepared by a simple and low-cost impregnation method, significantly improved the electrocatalytic activity of anode, thus contributing to tetracycline removal in the bio-electrocatalytic system, especially in the external coupling system.
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http://dx.doi.org/10.1007/s11356-022-21207-9DOI Listing
June 2022

Clinical Benefits of Direct-acting Antivirals Therapy in Hepatitis C Virus Patients with Hepatocellular Carcinoma: A Systematic Review and Meta-analysis.

J Gastroenterol Hepatol 2022 Jun 20. Epub 2022 Jun 20.

Department of General Surgery, Qilu Hospital, Shandong University, Jinan, China.

Purpose: The purpose of the present study was to evaluate the effect of direct-acting antivirals (DAAs) therapy on the clinical outcomes of hepatitis C virus (HCV) patients with hepatocellular carcinoma (HCC).

Methods: We searched multiple electronic databases from database inception to June 14, 2021. Meta-analyses were performed separately for HCC recurrence and overall survival (OS).

Results: A total of 23 studies were identified for the primary analysis. Compared with no intervention, pooled data showed significant benefit from DAAs therapy in reducing recurrence (adjusted HR = 0.55, 95% CI 0.41-0.74, p < 0.001; I = 66.6%, p < 0.001) and improving OS (adjusted HR = 0.36, 95% CI 0.16-0.83, p = 0.017; I = 90.7%, p < 0.001) of HCV-related HCC patients. Compared with non-responders, patients with sustained virologic response (SVR) had greater benefit from DAAs therapy in reducing recurrence (HR = 0.37, 95% CI 0.16-0.84, p = 0.017; I = 58.8%, p = 0.088) and improving OS (HR = 0.17; 95% CI 0.06-0.50; p = 0.001; I = 56.4%, p = 0.130). Though DAAs did not show significant advantages over IFN in reducing recurrence (adjusted HR =0.96, 95% CI 0.72-1.28, p = 0.784; I = 0.0%, p = 0.805), there seems to be a trend towards OS benefit from DAAs therapy (adjusted HR = 0.11, 95% CI 0.01-1.19, p = 0.059).

Conclusion: DAAs therapy can prevent recurrence and improve OS of HCV-related HCC patients, especially for patients with SVR. Further prospective randomized controlled trial is warranted to validate these results.
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http://dx.doi.org/10.1111/jgh.15915DOI Listing
June 2022

Robot-Assisted Laparoscopic Radical Cystectomy and Modified Y-Shaped Ileal Orthotopic Neobladder Reconstruction.

Front Surg 2022 1;9:889536. Epub 2022 Jun 1.

Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China.

Background: Orthotopic neobladder reconstruction has become the preferred method of urinary diversion after radical cystectomy in major medical centers. We performed modified Y-shaped ileal orthotopic neobladder reconstruction and presented the functional results and postoperative complications of the modified surgery.

Methods: We included 21 patients with bladder cancer who underwent radical cystectomy at our center between February 2019 and December 2019. All patients underwent robotic-assisted laparoscopic radical cystectomy and lymph node dissection plus modified Y-shaped ileal orthotopic neobladder reconstruction. We collected the demographic and pathological history of the patients, and perioperative and postoperative functional outcomes and postoperative complications were recorded.

Results: All surgeries were successful and no serious postoperative complications occurred. The mean operative time was 321.43 ± 54.75 min, including 101.67 ± 10.88 min required for neobladder reconstruction. Liquid intake was encouraged about 5 days after surgery, stent and catheter were removed after 13.52 ± 3.28 days, and the patients were discharged 1-2 days after removing the catheter. No ureteral anastomotic and neobladder urethral anastomotic strictures occurred. The volume of the neobladder at 1-year post-surgery was 195.24 ± 16.07 mL and the maximum urinary flow rate was 20.64 ± 2.22 mL/s.

Conclusion: We describe the robotic-assisted modified Y-shaped ileal orthotopic neobladder reconstruction performed at our center, which requires a simple suture and short neobladder construction time, minimizes the occurrence of anastomotic stenosis, facilitates smooth patient emptying, and is clinically scalable and applicable.
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http://dx.doi.org/10.3389/fsurg.2022.889536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198462PMC
June 2022

HSF1 Protects Sepsis-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome Activation.

Front Immunol 2022 1;13:781003. Epub 2022 Jun 1.

Sepsis Translational Medicine Key Laboratory of Hunan Province, Central South University, Changsha, China.

As an important transcription factor, heat shock factor 1 (HSF1) plays an endogenous anti-inflammation role in the body and can alleviate multiple organ dysfunction caused by sepsis, which contributes to an uncontrolled inflammatory response. The NLRP3 inflammasome is a supramolecular complex that plays key roles in immune surveillance. Inflammation is accomplished by NLRP3 inflammasome activation, which leads to the proteolytic maturation of IL-1β and pyroptosis. However, whether HSF1 is involved in the activation of the NLRP3 inflammasome in septic acute lung injury (ALI) has not been reported. Here, we show that HSF1 suppresses NLRP3 inflammasome activation in transcriptional and post-translational modification levels. HSF1 can repress NLRP3 expression inhibiting NF-κB phosphorylation. HSF1 can inhibit caspase-1 activation and IL-1β maturation promoting NLRP3 ubiquitination. Our finding not only elucidates a novel mechanism for HSF1-mediated protection of septic ALI but also identifies new therapeutic targets for septic ALI and related diseases.
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http://dx.doi.org/10.3389/fimmu.2022.781003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9199371PMC
June 2022

Gene Coexpression Network Characterizing Microenvironmental Heterogeneity and Intercellular Communication in Pancreatic Ductal Adenocarcinoma: Implications of Prognostic Significance and Therapeutic Target.

Front Oncol 2022 1;12:840474. Epub 2022 Jun 1.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Pancreatic ductal adenocarcinoma (PDAC) is characterized by intensive stromal involvement and heterogeneity. Pancreatic cancer cells interact with the surrounding tumor microenvironment (TME), leading to tumor development, unfavorable prognosis, and therapy resistance. Herein, we aim to clarify a gene network indicative of TME features and find a vulnerability for combating pancreatic cancer.

Methods: Single-cell RNA sequencing data processed by the Seurat package were used to retrieve cell component marker genes (CCMGs). The correlation networks/modules of CCMGs were determined by WGCNA. Neural network and risk score models were constructed for prognosis prediction. Cell-cell communication analysis was achieved by NATMI software. The effect of the ITGA2 inhibitor was evaluated by using a -driven murine pancreatic cancer model.

Results: WGCNA categorized CCMGs into eight gene coexpression networks. TME genes derived from the significant networks were able to stratify PDAC samples into two main TME subclasses with diverse prognoses. Furthermore, we generated a neural network model and risk score model that robustly predicted the prognosis and therapeutic outcomes. A functional enrichment analysis of hub genes governing gene networks revealed a crucial role of cell junction molecule-mediated intercellular communication in PDAC malignancy. The pharmacological inhibition of ITGA2 counteracts the cancer-promoting microenvironment and ameliorates pancreatic lesions .

Conclusion: By utilizing single-cell data and WGCNA to deconvolute the bulk transcriptome, we exploited novel PDAC prognosis-predicting strategies. Targeting the hub gene ITGA2 attenuated tumor development in a PDAC mouse model. These findings may provide novel insights into PDAC therapy.
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http://dx.doi.org/10.3389/fonc.2022.840474DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198606PMC
June 2022

Death Attitudes and Death Anxiety Among Medical Interns After the 2020 Outbreak of the Novel Coronavirus.

Authors:
Yiqing He Tao Li

Front Psychol 2022 3;13:698546. Epub 2022 Jun 3.

Department of Radiology, Shenzhen Maternity and Child Healthcare Hospital, Shenzhen, China.

This study investigates the and influencing factors of death attitudes and death anxiety among medical interns in China as measured by the and following the outbreak of "Novel Coronavirus Pneumonia" in China in early 2020. Results of this study show that under the influence of COVID-19, in terms of death attitude, medical interns scored the highest in neutral acceptance and the lowest in escape acceptance. There were significant differences in death attitude and anxiety among the groups with different backgrounds, including their families' approaches to discussions of death, the number of funeral experiences, and other factors. There were two additional factors affecting attitudes that were related to the epidemic situation: whether the individual had participated in work to treat COVID-19 and whether their close friends or relatives ("cherished persons") had been diagnosed with COVID-19. The study reveals the ways that the epidemic had an impact on death attitude and death anxiety.
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http://dx.doi.org/10.3389/fpsyg.2022.698546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204218PMC
June 2022

[Research of preparation quality markers of Yulian Tang with anti-inflammatory activity].

Zhongguo Zhong Yao Za Zhi 2022 Jun;47(11):2947-2954

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

The lipopolysaccharide(LPS)-indused RAW264.7 cells inflammation model was used as a carrier to investigated the effects of the preparation quality markers of Yulian Tang with anti-inflammatory activity in vitro. RAW264.7 cells were treated with LPS(50 ng·mL~(-1)) or/and different concentrations(low dose 0.1 μmol·L~(-1); medium dose 1 μmol·L~(-1); high dose 10 μmol·L~(-1)) of 18 chemical components in Yulian Tang for 24 h. Then the activity of RAW264.7 cell was detected using Cell Counting Kit-8(CCK-8) and the concentrations of inflammatory factors TNF-α and IL-6 in the supernatant of RAW264.7 cell were detected by ELISA assay. As the concentrations of chemical components in Yulian Tang increased, berberine, coptisine, magnoflorine, epiberberine, columbamine and costunolide had stronger inhibitory effects on TNF-α, whereas limonin, dehydroevodiamine, chlorogenic acid, neochlorogenic acid, groenlandicine, evodiamine, rutaecarpine and phellodendrine showed weakened inhibitory effects on TNF-α. The concentrations of palmatine, jatrorrhizine, dehydrocostus lactone and cryptochlorogenic acid had no significant effect on their inhibitory effect on TNF-α. Furthermore, dehydrorutaecarpine, chlorogenic acid, neochlorogenic acid, evodiamine, rutaecarpine, costunolide, phellodendrine and cryptochlorogenic acid showed stronger inhibitory effect on IL-6 as their concentrations increased; berberine, coptisine, magnoflorine, epiberberine, limonin, columbamine, groenlandicine and dehydrocostus lactone had no changes in their inhibitory effects on IL-6 as the concentrations increased. Palmatine and jatrorrhizine had the best inhibitory effect on IL-6. Combining the previous analysis of qualitative and quantitative preparation quality markers of Yulian Tang with the above result of dose-response relationship, we finally identified 15 preparation quality markers of Yulian Tang with anti-inflammatory activity, namely berberine, coptisine, palmatine, magnoflorine, epiberberine, limonin, columbamine, jatrorrhizine, neochlorogenic acid, chlorogenic acid, groenlandicine, evodiamine, rutaecarpine, dehydrocostus lactone and costunolide. In conclusion, our study provides a quick strategy for screening the qualitative preparation quality markers of Yulian Tang with anti-inflammatory activity. Moreover, it also provides an explicit route for the determination of preparation quality markers of Yulian Tang with other activities.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220118.301DOI Listing
June 2022

Comparative transcriptome analysis of differentially expressed genes and pathways in Procambarus clarkii (Louisiana crawfish) at different acute temperature stress.

Genomics 2022 Jun 16:110415. Epub 2022 Jun 16.

Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy ofFishery Sciences, Shenzhen 518108, China; Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China. Electronic address:

Procambarus clarkii is an important economic species in China, and exhibit heat and cold tolerance in the main culture regions. To understand the mechanisms, we analyzed the hepatopancreas transcriptome of P. clarkii treated at 10 °C, 25 °C, and 30 °C, then 2092 DEGs and 6929 DEGs were found in 30 °C stress group and 10 °C stress group, respectively. KEGG pathway enrichment results showed that immune pathway is the main stress pathway for 10 °C treatment and metabolic pathway is the main response pathway for 30 °C treatment, which implies low temperature stress induces the damage of the immune system and increases the susceptibility of bacteria while the body response to high temperature stress through metabolic adjustment. In addition, flow cytometry proved that both high and low temperature stress caused different degrees of apoptosis of hemocytes, and dynamic transcription heat map analysis also identified the differential expression of HSPs family genes and apoptosis pathway genes under different heat stresses. This indicates that preventing damaged protein misfolding and accelerating cell apoptosis are necessary mechanisms for P. clarkii to cope with high and low temperature stress. Our research has deepened our understanding of the complex molecular mechanisms of P. clarkii in response to acute temperature stress, and provided a potential strategy for aquatic animals to relieve environmental duress.
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http://dx.doi.org/10.1016/j.ygeno.2022.110415DOI Listing
June 2022

A Novel Stress State Assessment Method for College Students Based on EEG.

Comput Intell Neurosci 2022 7;2022:4565968. Epub 2022 Jun 7.

Jiangsu Vocational College of Information Technology, Wuxi, Jiangsu 214153, China.

Stress is an unavoidable problem for today's college students. Stress can arouse strong personal emotional and behavioral responses. Compared with other groups of the same age, college students have a special way of life and living environment. They have complex interpersonal relationships and relatively weak social support systems. At the same time, they also face fierce competition in both academic and employment. However, they lack the skills to deal with the crisis and are reluctant to ask others for help, which leads to a simultaneous increase in mental stress. The pressure on college students mainly comes from study, family, social, employment, society, and economy. When students face multiple pressures from family, school, society, etc., some students are prone to some psychological problems due to their own personality or external environment and other reasons. Therefore, regular assessment of students' stress status is an important means to prevent college students' psychological problems. Considering that in real life, the number of students whose pressure is within the tolerable range is the majority, while the number of students who are under too much pressure is a minority. Therefore, the actual dataset to be identified belongs to a kind of imbalanced data. In this study, an improved extreme learning machine (IELM) is used to improve the performance of the recognition model as much as possible. IELM takes the idea of label weighting as the starting point, introduces the AdaBoost algorithm, and combines its weight distribution with the label weighted extreme learning machine (ELM). During the weight update process, the advantage of the imbalanced nature of multi-label datasets is taken. IELM was used to classify EEG data to determine the stress level of college students. The experimental results demonstrate that the algorithm used in this study has excellent classification performance and can accurately assess students' stress levels. The accurate assessment of stress has provided a solid foundation for the development of students' mental health and has significant practical implications.
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http://dx.doi.org/10.1155/2022/4565968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197644PMC
June 2022

Retraction notice to "Iron chelator-induced up-regulation of Ndrg1 inhibits proliferation and EMT process by targeting Wnt/β-catenin pathway in colon cancer cells" [Biochem. Biophys. Res. Commun. 506/1 (2018) 114-121].

Biochem Biophys Res Commun 2022 Jul;615:172

Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, PR China. Electronic address:

This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). The Editor-in-Chief has decided to retract this paper as the results cannot be relied upon. The authors contacted the editorial office admitting to outsourcing some of the experiments, specifically identifying that the Western Blot images were generated by a third-party laboratory. The authors tried to validate the results of the paper and obtain the raw data but were unable to do so, and offered to run additional experiments to correct the paper. The authors claim that the results and conclusions are still valid. The authors utilised potentially fraudulent data to generate their conclusions in this paper, which is in significant violation of journal policies and scientific best practice. The paper is therefore retracted.
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http://dx.doi.org/10.1016/j.bbrc.2022.05.097DOI Listing
July 2022

Dendrites use mechanosensitive channels to proofread ligand-mediated neurite extension during morphogenesis.

Dev Cell 2022 Jun 8. Epub 2022 Jun 8.

Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, CA, USA. Electronic address:

Ligand-receptor interactions guide axon navigation and dendrite arborization. Mechanical forces also influence guidance choices. However, the nature of such mechanical stimulations, the mechanosensor identity, and how they interact with guidance receptors are unknown. Here, we demonstrate that mechanosensitive DEG/ENaC channels are required for dendritic arbor morphogenesis in Caenorhabditis elegans. Inhibition of DEG/ENaC channels causes reduced dendritic outgrowth and branching in vivo, a phenotype that is alleviated by overexpression of the mechanosensitive channels PEZO-1/Piezo or YVC1/TrpY1. DEG/ENaCs trigger local Ca transients in growing dendritic filopodia via activation of L-type voltage-gated Ca channels. Anchoring of filopodia by dendrite ligand-receptor complexes is required for the mechanical activation of DEG/ENaC channels. Therefore, mechanosensitive channels serve as a checkpoint for appropriate chemoaffinity by activating Ca transients required for neurite growth.
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http://dx.doi.org/10.1016/j.devcel.2022.05.019DOI Listing
June 2022

Calcium-Differentiated Cellular Internalization of Allosteric Framework Nucleic Acids for Targeted Payload Delivery.

Anal Chem 2022 Jun 16. Epub 2022 Jun 16.

Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China.

Target delivery systems have extensively shown promising applications in cancer therapy, and many of them function smartly by responding to the cancer cell microenvironment. Here, we for the first time report Ca-differentiated cellular internalization of 2D/3D framework nucleic acids (FNAs), enabling the engineering of a conceptually new target delivery system using an allosteric FNA nanovehicle. The FNA vehicle is subject to a 2D-to-3D transformation on the cancer cell surface G-quadruplexes responding to environmental K and thereby allows its cell entry to be more efficiently promoted by Ca. This design enables the FNA vehicle to target cancer cells and selectively deliver an antisense strand-containing cargo for live-cell mRNA imaging. It would open new avenues toward targeted drug delivery and find extensive applications in precise disease treatment.
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http://dx.doi.org/10.1021/acs.analchem.2c01434DOI Listing
June 2022

Alternate-Day Ketogenic Diet Feeding Protects against Heart Failure through Preservation of Ketogenesis in the Liver.

Oxid Med Cell Longev 2022 6;2022:4253651. Epub 2022 Jun 6.

Department of Rehabilitation Medicine, China-Japan Friendship Hospital, Beijing 100029, China.

As heart failure develops, the heart utilizes ketone bodies at increased rates, indicating an adaptive stress response. Thus, increasing ketone body availability exerts protective effects against heart failure. However, although it is the widely used approach for increasing ketone body availability, the ketogenic diet shows limited cardioprotective effects against heart failure. This study was aimed at examining the effects of the ketogenic diet on heart failure and the underlying mechanisms. Pressure overload-induced heart failure was established by transverse aortic constriction (TAC) in mice. Continuous ketogenic diet feeding for 8 weeks failed to protect the heart against heart failure. It showed no significant effects on cardiac systolic function and fibrosis but aggravated cardiac diastolic function in TAC mice. Specifically, it induced systemic lipid metabolic disorder and hepatic dysfunction in TAC mice. It decreased the content of 3-hydroxy-3-methylglutaryl-CoA lyase (HMGCL), a key enzyme in ketogenesis, and impaired the capacity of hepatic ketogenesis in TAC mice. It preserved the capacity of hepatic ketogenesis and exerted cardioprotective effects against heart failure, increasing cardiac function and decreasing cardiac fibrosis, in liver-specific HMGCL-overexpressed TAC mice. Importantly, we found that alternate-day ketogenic diet feeding did not impair the capacity of hepatic ketogenesis and exerted potent cardioprotective effects against heart failure. These results suggested that alternate-day but not continuous ketogenic diet protects against heart failure through preservation of ketogenesis in the liver.
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http://dx.doi.org/10.1155/2022/4253651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192193PMC
June 2022

Generation and characterization of human-derived iPSC lines from two cousins with schizophrenia and bipolar disorder and their unaffected cousin.

Stem Cell Res 2022 Jun 7;63:102832. Epub 2022 Jun 7.

Department of Neurobiology, Affiliated Mental Health Center & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou 310013, Zhejiang, China; NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address:

Schizophrenia (SCZ) and bipolar disorder (BD) are debilitating neurodevelopmental disorders with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by three females. An adolescent female was clinically diagnosed as first-episode SCZ. One of her cousins was clinically diagnosed as BD and another one was unaffected control. Induced pluripotent stem cells (iPSCs) were established with reprograming factors Oct4, Sox2, Nanog, Lin28, c-myc, Klf4, and SV40LT. All lines presented normal karyotype and highly expressed pluripotency markers in vitro. All iPSCs were capable to differentiate into derivatives of three germ layers in vivo.
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http://dx.doi.org/10.1016/j.scr.2022.102832DOI Listing
June 2022

A novel maize microRNA negatively regulates resistance to Fusarium verticillioides.

Mol Plant Pathol 2022 Jun 14. Epub 2022 Jun 14.

College of Life Sciences, Henan Agricultural University, Zhengzhou, China.

Although microRNAs (miRNAs) regulate the defence response against multiple pathogenic fungi in diverse plant species, few efforts have been devoted to deciphering the involvement of miRNA in resistance to Fusarium verticillioides, a major pathogenic fungus affecting maize production. In this study, we discovered a novel F. verticillioides-responsive miRNA designated zma-unmiR4 in maize kernels. The expression of zma-unmiR4 was significantly repressed in the resistant maize line but induced in the susceptible lines upon exposure to F. verticillioides exposure, whereas its target gene ZmGA2ox4 exhibited the opposite pattern of expression. Heterologous overexpression of zma-unmiR4 in Arabidopsis resulted in enhanced growth and compromised resistance to F. verticillioides. By contrast, transgenic plants overexpressing ZmGA2ox4 or the homologue AtGA2ox7 showed impaired growth and enhanced resistance to F. verticillioides. Moreover, zma-unmiR4-mediated suppression of AtGA2ox7 disturbed the accumulation of bioactive gibberellin (GA) in transgenic plants and perturbed the expression of a set of defence-related genes in response to F. verticillioides. Exogenous application of GA or a GA biosynthesis inhibitor modulated F. verticillioides resistance in different plants. Taken together, our results suggest that the zma-unmiR4-ZmGA2ox4 module might act as a major player in balancing growth and resistance to F. verticillioides in maize.
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http://dx.doi.org/10.1111/mpp.13240DOI Listing
June 2022
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