Publications by authors named "Tao Li"

4,560 Publications

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Redox-neutral photochemical Heck-type arylation of vinylphenols activated by visible light.

Chem Sci 2020 Jan 10;11(8):2130-2135. Epub 2020 Jan 10.

Key Laboratory of Medicinal Chemistry for Natural Resource (Ministry of Education and Yunnan Province), State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Chemical Science and Technology, Library of Yunnan University, Yunnan University 2 North Cuihu Road Kunming 650091 China

Disclosed herein is a photochemical Heck-type arylation of vinylphenols with non-activated aryl and heteroaryl halides under visible light irradiation. Preliminary mechanistic studies suggested that the colored vinylphenolate anions acted as a strong reducing photoactivator to directly activate (hetero)aryl halides without the need for any sacrificial reductants. The photochemically generated aryl radicals coupled with another molecule of vinylphenol to afford the Heck-type arylation product in a regiospecific and stereoselective manner. The developed photochemical arylation protocol showed exceptional functional group tolerance and was successfully applied in the challenging late-stage modification of natural products without any protection-deprotection procedures.
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http://dx.doi.org/10.1039/c9sc06184cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150107PMC
January 2020

Engineering plasticization resistant gas separation membranes using metal-organic nanocapsules.

Chem Sci 2020 Apr 15;11(18):4687-4694. Epub 2020 Apr 15.

School of Physical Science and Technology, ShanghaiTech University Shanghai 201210 China

Membrane technologies hold great potential for industrial gas separation. Nevertheless, plasticization, a common phenomenon that is responsible for the loss of gas pair selectivity and the decrease of membrane lifespan, is one of the top challenges withholding the deployment of advanced membrane materials in realistic applications. Here, we report a highly generalizable approach, that utilizes PgCCu, a copper metal-organic nanocapsule (MONC) containing 24 open metal sites (OMSs) as a multi-dentate node to coordinatively crosslink polymers. By adding merely 1-3 wt% of PgCCu, a wide range of carbonyl group-containing polymers can be effectively crosslinked. Through rigorous dissolution tests, molecular dynamic simulations, and FT-IR spectroscopy, we qualitatively and quantitatively unveiled the coordinative binding nature at the polymer-MONC interface. As a result, we produced a series of composite membranes showing near complete plasticization resistance to CO, CH, and CH under high pressure with no loss of mechanical and gas transport properties.
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http://dx.doi.org/10.1039/d0sc01498bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159238PMC
April 2020

Brain structural alterations in MDD patients with gastrointestinal symptoms: Evidence from the REST-meta-MDD project.

Prog Neuropsychopharmacol Biol Psychiatry 2021 Jun 11;111:110386. Epub 2021 Jun 11.

Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

Objective: While gastrointestinal (GI) symptoms are very common in patients with major depressive disorder (MDD), few studies have investigated the neural basis behind these symptoms. In this study, we sought to elucidate the neural basis of GI symptoms in MDD patients by analyzing the changes in regional gray matter volume (GMV) and gray matter density (GMD) in brain structure.

Method: Subjects were recruited from 13 clinical centers and categorized into three groups, each of which is based on the presence or absence of GI symptoms: the GI symptoms group (MDD patients with at least one GI symptom), the non-GI symptoms group (MDD patients without any GI symptoms), and the healthy control group (HCs). Structural magnetic resonance images (MRI) were collected of 335 patients in the GI symptoms group, 149 patients in the non-GI symptoms group, and 446 patients in the healthy control group. The 17-item Hamilton Depression Rating Scale (HAMD-17) was administered to all patients. Correlation analysis and logistic regression analysis were used to determine if there was a correlation between the altered brain regions and the clinical symptoms.

Results: There were significantly higher HAMD-17 scores in the GI symptoms group than that of the non-GI symptoms group (P < 0.001). Both GMV and GMD were significant different among the three groups for the bilateral superior temporal gyrus, bilateral middle temporal gyrus, left lingual gyrus, bilateral caudate nucleus, right Fusiform gyrus and bilateral Thalamus (GRF correction, cluster-P < 0.01, voxel-P < 0.001). Compared to the HC group, the GI symptoms group demonstrated increased GMV and GMD in the bilateral superior temporal gyrus, and the non-GI symptoms group demonstrated an increased GMV and GMD in the right superior temporal gyrus, right fusiform gyrus and decreased GMV in the right Caudate nucleus (GRF correction, cluster-P < 0.01, voxel-P < 0.001). Compared to the non-GI symptoms group, the GI symptoms group demonstrated significantly increased GMV and GMD in the bilateral thalamus, as well as decreased GMV in the bilateral superior temporal gyrus and bilateral insula lobe (GRF correction, cluster-P < 0.01, voxel-P < 0.001). While these changed brain areas had significantly association with GI symptoms (P < 0.001), they were not correlated with depressive symptoms (P > 0.05). Risk factors for gastrointestinal symptoms in MDD patients (p < 0.05) included age, increased GMD in the right thalamus, and decreased GMV in the bilateral superior temporal gyrus and left Insula lobe.

Conclusion: MDD patients with GI symptoms have more severe depressive symptoms. MDD patients with GI symptoms exhibited larger GMV and GMD in the bilateral thalamus, and smaller GMV in the bilateral superior temporal gyrus and bilateral insula lobe that were correlated with GI symptoms, and some of them and age may contribute to the presence of GI symptoms in MDD patients.
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http://dx.doi.org/10.1016/j.pnpbp.2021.110386DOI Listing
June 2021

A Fast PCR Test for the Simultaneous Identification of Species and Gender in Horses, Donkeys, Mules and Hinnies.

J Equine Vet Sci 2021 Jul 27;102:103458. Epub 2021 Apr 27.

College of animal science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, China. Electronic address:

Having considered that the current methods are costly and time-consuming, we designed an only 3 pairs primer-based PCR test to accurately identify the species and gender in horses, donkeys, mules and hinnies. Through a thorough sequence comparison between horse and donkey's highly similar genomes, and a vast amount of preliminary confirmation, we found that three fragments, CNGB3 gene on an autosome, displacement loop region on mitochondrial DNA and SRY genes on chromosome Y, within these equine DNA, are enough to enable us achieving our goal. The PCR test described here would be an economical, fast and accurate alternative for the most commonly-used methods, polymerase chain reaction-restriction fragment length polymorphism, microsatellite assay, and sequencing.
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http://dx.doi.org/10.1016/j.jevs.2021.103458DOI Listing
July 2021

Simultaneous transcatheter treatment of ascending aortic aneurysm with aortic and mitral regurgitation: an in vitro study.

Interact Cardiovasc Thorac Surg 2021 Jun 11. Epub 2021 Jun 11.

Department of Vascular Surgery, Changhai Hospital, Endovascular Diagnosis and Treatment Center for Heart Valvular Diseases, and Endovascular Diagnosis and Treatment Center for Aortic Dissection, Navy Medical University, Shanghai, China.

Objectives: We sought to explore the efficacy of the endovascular repair of an ascending aortic aneurysm with aortic and mitral regurgitation by 2 novel valved stents.

Methods: We established models of ascending aortic aneurysms combined with aortic and mitral regurgitation in 10 pig hearts, then implanted self-expanding aortic fenestrated and mitral valved stents via the transapical approach. We applied a fluoroscopy-guided in vitro setting to test the approach, then analysed continuous circulating flushing at 37°C. Finally, we determined operating times, echocardiography and changes of coronary flow as well as fenestration alignment with the coronary ostia.

Results: This approach resulted in a 100% overall technical success rate, excellent handling properties and precise positioning. The time taken to implant the 2 valved stents was 59 ± 12 min. Flow of the left and right coronary arteries did not significantly decrease after the stents were implanted (330.4 ± 12.06 ml/min vs 289.4 ± 5.29 ml/min, P < 0.001; 376.8 ± 10.5 ml/min vs 350.0 ± 14.5 ml/min; P < 0.001). We found no obvious regurgitation and perivalvular leakage; nor did the gradients of the aortic and mitral valves as well as of the left ventricular outflow tract increase significantly. The final angiographic examination and profile of the coronary opening confirmed the good position of the valved stents, the exclusion of the aneurysm and the patency of both coronary arteries.

Conclusions: These findings indicate the potential for combined transcatheter aortic root and mitral valve replacement in treating aortic root pathologies. In future, in vivo studies are expected to validate this approach and ascertain its durability.
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http://dx.doi.org/10.1093/icvts/ivab101DOI Listing
June 2021

High rate of completion for weekly rifapentine plus isoniazid treatment in Chinese children with latent tuberculosis infection-A single center study.

PLoS One 2021 11;16(6):e0253159. Epub 2021 Jun 11.

Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Three months of weekly rifapentine plus isoniazid (3HP) is a short course regimen for latent tuberculosis infection treatment with satisfied safety and efficacy. However, research on its use in children is limited. In this study, we evaluated the completion rate and safety of the 3HP regimen among children in China. Participants aged 1-14 years receiving 3HP for TB prevention at Shanghai Public Health Clinical Center were followed from December 2019 to November 2020 to evaluate the safety and completion rate of the treatment. Thirty-one children were eligible for inclusion, but five were excluded from the analysis (three were treated with a lower than recommended dose, and two were lost to follow-up). Of the 26 children included in the analysis, the treatment completion rate was 100%. Adverse drug reactions (ADRs) were reported in 38.5% (10/26) of the patients. The most common ADRs were gastrointestinal symptoms (19.2%,5/26), and all ADRs were rated as Grade 1. The 3HP regimen has a high completion rate, and it seems well tolerated in our study population. However, further randomized controlled clinical trial with larger sample size are warranted.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253159PLOS
June 2021

Theoretical and experimental investigations on Nb2CTx MXene Q-switched Tm:YAP laser at 2 μm for the nonlinear optical response.

Nanotechnology 2021 Jun 9. Epub 2021 Jun 9.

Shandong University, Qingdao, Shandong, CHINA.

In this paper, the Nb2CTx MXene nanosheets were fabricated and the corresponding microstructures were investigated. The nonlinear optical response was illustrated by open aperture Z-scan and I-scan methods. The ground and the excited state absorption cross-sections of 2D Nb2CTx MXene were also investigated. As the saturable absorber (SA), the Nb2CTx MXene was applied in the passively Q-switched Tm:YAP laser. 1.96-μs Q-switched pulses with 3.97-W peak power were achieved at the repetition frequency of 80 kHz. Further theoretical model was built by using the coupled rate equations in simulating the dynamic process of the passively Q-switched Tm:YAP laser. The numerical simulation results are fundamentally in agreement with the experimental results, which proves the Nb2CTx MXene can be a good potential nanomaterial for further optoelectronic applications.
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http://dx.doi.org/10.1088/1361-6528/ac09a8DOI Listing
June 2021

Prognostic value of plasma IL-27 on biological viability of hepatic cystic echinococcosis patients.

Int J Infect Dis 2021 Jun 6. Epub 2021 Jun 6.

Department of Liver & Laparoscopic Surgery, Digestive & Vascular Surgery Center, 1(st)Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China; State Key Laboratory of Pathogenesis, Prevention, Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi 830054, China. Electronic address:

Objectives: We aimed to investigate the potential biomarker for distinguishing biological viability of hepatic echinococcosis.

Methods: We measured plasma concentrations of several cytokines and chemokines in patients with active and non-active cysts (HCE, n = 47), stable/progressive alveolar echinococcosis (HAE, n = 38) patients and their comparable infection-free volunteers (n = 48) through Luminex assay technique. Disease progression was staged according to the classification standard.

Results: Compared to healthy controls, enhanced elevation of Th22 type cytokine IL-22 and chemokines Eotaxin, IP-10, MCP-1, SDF-1α concentrations in HAE patients, IL-22, GROα, MCP-1, RANTES, SDF-1α concentrations in HCE patients were found (p < 0.05-0.001). For those with HCE patients, only IL-27 concentrations in non-active HCE were profoundly lower than it in active HCE. Results of logistic regression analysis showed IL-27 less than 20.79pg/mL as an independent risk factor for HCE biological viability when ROC analysis at cut-off value of 44.23pg/mL resulted in AUC value of 0.72.

Conclusions: Our present findings supply with multiple cytokine and chemokine secretion patterns in both HAE and HCE patients with different progressive stages. IL-27 could be served as a referring biomarker for distinguishing HCE biological viability and provide with preliminary theoretical foundation to clinical decision.
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http://dx.doi.org/10.1016/j.ijid.2021.06.003DOI Listing
June 2021

Atrial reconstruction, distal gastrectomy with Ante-situm liver resection and autotransplantation for hepatocellular carcinoma with atrial tumor thrombus: A case report.

Medicine (Baltimore) 2021 May;100(19):e25780

Department of Liver Transplantation & Laparoscopic Surgery, Center of Organ Transplantation, The 1st Affiliated Hospital of Xinjiang Medical University.

Rationale: Hepatocellular with tumor thrombi extending into 3 hepatic veins (HVs) and right atrium presents as a real clinical challenge. We report the first documented case of surgical resection of an advanced hepatocellular carcinoma (HCC) with extensive invasion to distal stomach, atrium and hepatic vasculatures.

Patient Concerns: We present a case of 48-years old man with abdominal mass accompanying shortness of breath after activities.

Diagnoses: Preoperative examination revealed giant HCC with tumor thrombi extending into portal vein, HVs, inferior vena cava, and atrium.

Interventions: Distal stomach involvement was confirmed at surgery and, distal gastrectomy, atrial reconstruction and ante-situm liver resection and autotransplantation under cardio-pulmonary bypass were performed.

Outcomes: The operation time was 490 minutes, extracorporeal circulation time 124 minutes, and anhepatic time 40 minutes. Postoperative follow-up revealed normal hepatic and cardiac function with no sign of recurrence.

Lessons: This case illustrates that the extensive invasion of HCC to major vasculature and adjacent organs may not necessarily preclude the liver autotransplantation with multi-visceral resection as the treatment option of extremely advanced HCC patients.
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http://dx.doi.org/10.1097/MD.0000000000025780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133267PMC
May 2021

The complete mitochondrial genome of (Acari: Acaridae).

Mitochondrial DNA B Resour 2021 May 19;6(6):1680-1681. Epub 2021 May 19.

Department of Health Inspection and Quarantine, Wannan Medical College, Wuhu, China.

We assembled and annotated the complete mitochondrial genome of . It is the first complete mitochondrial genome sequence from the genus . The mitogenome was 13,966 bp in length and contains 37 genes (including 13 protein-coding genes, 22 transfer RNA (tRNA), and two ribosomal RNA (rRNA)), and one largest non-coding region. The gene arrangement of is consistent with the pattern of possible common ancestor of astigmatid mites. In the present study, phylogenetic analysis shows that genus was clustered into one branch with other Acaridae species.
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http://dx.doi.org/10.1080/23802359.2021.1927874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143620PMC
May 2021

Unique and universal dew-repellency of nanocones.

Nat Commun 2021 06 8;12(1):3458. Epub 2021 Jun 8.

Physique et Mécanique des Milieux Hétérogènes, UMR 7636 du CNRS, ESPCI, PSL Research University, Paris, France.

Surface structuring provides a broad range of water-repellent materials known for their ability to reflect millimetre-sized raindrops. Dispelling water at the considerably reduced scale of fog or dew, however, constitutes a significant challenge, owing to the comparable size of droplets and structures. Nonetheless, a surface comprising nanocones was recently reported to exhibit strong anti-fogging behaviour, unlike pillars of the same size. To elucidate the origin of these differences, we systematically compare families of nanotexture that transition from pillars to sharp cones. Through environmental electron microscopy and modelling, we show that microdroplets condensing on sharp cones adopt a highly non-adhesive state, even at radii as low as 1.5 µm, contrasting with the behaviour on pillars where pinning results in impedance of droplet ejection. We establish the antifogging abilities to be universal over the range of our cone geometries, which speaks to the unique character of the nanocone geometry to repel dew. Truncated cones are finally shown to provide both pinning and a high degree of hydrophobicity, opposing characteristics that lead to a different, yet efficient, mechanism of dew ejection that relies on multiple coalescences.
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http://dx.doi.org/10.1038/s41467-021-23708-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187394PMC
June 2021

Endothelial nitric oxide synthase polymorphism and venous thromboembolism: A meta-analysis of 9 studies involving 3993 subjects.

Phlebology 2021 Jun 9:2683555211016626. Epub 2021 Jun 9.

Department of Trauma Surgery, Emergency Medical Cental of Chongqing, The Affiliated Central Hospital of Chongqin University, Chongqing, China.

Background: Endothelial nitric oxide synthase (eNOS) polymorphism may influence the risk of venous thromboembolism (VTE). However, data from published studies with low statistical power are inconclusive. The present meta-analysis aimed to assess the relationship between eNOS polymorphism and the risk of VTE.

Method: Case-control studies evaluating the association between the eNOS polymorphism and VTE were searched in PubMed, Embase, Web of Science, Google Scholar, Wanfang, Chinese National Knowledge Infrastructure (CNKI), the Chongqing VIP Chinese Science and Technology Periodical Database (VIP), and Chinese Biomedical Literature Database (CBM).

Results: A total of 1588 cases and 2405 controls from 9 studies were included in the analysis. The results showed that eNOS G894T polymorphism was related to VTE susceptibility and the difference was statistically significant [T vs G: OR = 1.41, 95% CI (1.13, 1.75),  = 0.002; TT + GG vs TG: OR = 0.71, 95% CI (0.60, 0.84),  = 0.000; TT + TG vs GG: OR = 1.45, 95% CI (1.23, 1.70),  = 0.000]. Additionally, eNOS Intron 4 VNTR polymorphism was related to VTE susceptibility and the difference was statistically significant [4b4b vs 4a4a + 4a4b: OR = 2.77, 95% CI (1.01, 7.61),  = 0.048].

Conclusion: ENOS G894T and eNOS Intron 4 VNTR polymorphisms were associated with VTE susceptibility, especially in Asian populations. However, multicenter studies with larger samples should be conducted to further clarify this association and verify our findings.
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http://dx.doi.org/10.1177/02683555211016626DOI Listing
June 2021

LB100 attenuates methamphetamine-induced behavioral sensitization by inhibiting the Raf1-ERK 1/2 cascade in the caudate putamen.

Neuroreport 2021 Jun 7. Epub 2021 Jun 7.

College of Forensic Medicine, Xi'an Jiaotong University Health Science Center Institute of Forensic Injury, Institute of Forensic Bioevidence, Western China Science and Technology Innovation Harbor, Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.

Methamphetamine (METH) abuse has become a serious social problem. Behavioral sensitization is a common behavioral paradigm used to study the neurobiological mechanism that underlies drug addiction. Our previous study demonstrated that the activity of protein phosphatase 2A (PP2A) and the level of phosphorylated extracellular signal-related kinase 1/2 (p-ERK 1/2) are increased in the caudate putamen (CPu) of METH-sensitive mice. However, the relationship between PP2A and ERK 1/2 in METH-induced behavioral sensitization remains unknown. Some studies have indicated that Raf1 may be involved in this process. In this study, LB100, a PP2A inhibitor for treating solid tumors, was first used to clarify the relationship between PP2A and ERK 1/2. In addition, Western blot was used to examine the levels of p-Raf1 (Ser 259) and p-ERK 1/2 (Thr 202/Tyr 204) in the CPu, hippocampus (Hip) and nucleus accumbens (NAc). Our results showed that 2 mg/kg LB100 significantly attenuated METH-induced behavioral sensitization. Furthermore, Western blot analysis revealed that pretreatment with 2 mg/kg LB100 remarkably reversed METH-induced reduction of p-Raf1, as well as upregulation of p-ERK 1/2 in the CPu. Taken together, these results indicate that PP2A plays an important role in METH-induced behavioral sensitization and phosphorylates ERK 1/2 by dephosphorylating p-Raf1 in the CPu to further regulate METH-induced behavioral sensitization.
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http://dx.doi.org/10.1097/WNR.0000000000001678DOI Listing
June 2021

lncRNA MIAT/HMGB1 Axis Is Involved in Cisplatin Resistance Regulating IL6-Mediated Activation of the JAK2/STAT3 Pathway in Nasopharyngeal Carcinoma.

Front Oncol 2021 20;11:651693. Epub 2021 May 20.

Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, China.

Cisplatin-based chemotherapy and radiotherapy are the main first-line treatment strategies for nasopharyngeal carcinoma (NPC) patients. Unfortunately, resistance is a major obstacle in the clinical management of NPC patients. We prove that the expression level of high-mobility group box 1 (HMGB1) is dramatically increased in resistant NPC cells than that in sensitive cells. HMGB1 induces the expression and secretion of IL6, which leads to constitutive autocrine activation of the JAK2/STAT3 pathway and eventually contributes to chemoresistance in NPC cells. Long non-coding RNAs (lncRNAs) have been identified as key regulators involved in drug resistance. In this study, using GO analysis of the biological process and differential expression analysis, we find 12 significantly altered IncRNAs in NPC cell lines, which may be involved in regulating gene expression. Furthermore, we determine that elevated lncRNA MIAT level upregulates HMGB1 expression, contributing to cisplatin resistance in NPC cells. We find that the deficiency of the lncRNA MIAT/HMGB1 axis, inhibition of JAK2/STAT3, or neutralization of IL6 by antibodies significantly re-sensitizes resistant NPC cells to cisplatin in resistant NPC cells. Moreover, we provide the evidence that the deficiency of HMGB1 reduces cisplatin-resistant tumor growth. Most importantly, we provide clinical evidence showing that the expression level of the lncRNA MIAT/HMGB1/IL6 axis is elevated in resistant NPC tumors, which is highly correlated with poor clinical outcome. Our findings identify a novel chemoresistance mechanism regulated by the lncRNA MIAT/HMGB1/IL6 axis, which indicates the possibilities for lncRNA MIAT, HMGB1, and IL6 as biomarkers for chemoresistance and targets for developing novel strategies to overcome resistance in NPC patients.
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http://dx.doi.org/10.3389/fonc.2021.651693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173225PMC
May 2021

RECK expression is associated with angiogenesis and immunogenic Tumor Microenvironment in Hepatocellular Carcinoma, and is a prognostic factor for better survival.

J Cancer 2021 5;12(13):3827-3840. Epub 2021 May 5.

Department of general surgery, Qilu Hospital, Shandong University, Jinan 250012, China.

Angiogenesis and immunosuppression have been described as closely related processes that can occur in parallel. As an inhibitor of matrix metalloproteinase, whether the level of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) in hepatocellular carcinoma (HCC) reflects a link between angiogenesis and immunosuppression is still unknown. We analyzed RNA expression, immune infiltration and survival of HCC from The Cancer Genome Atlas databases. Immune scores and stromal scores were calculated based on the ESTIMATE algorithm to quantify the immune and stromal components in HCC. The association between RECK and clinicopathological features was further investigated by immunohistochemistry on tissue microarray. We found that the prognosis of patients with high RECK expression was significantly better than that of patients with low RECK expression. High RECK expression was associated with high ESTIMATE Score, recruitment of more tumor-infiltrating lymphocytes, low tumor purity, and high PD-L1 expression. In addition, positive RECK expression was associated with a lower incidence of vascular invasion and recurrence, a lower level of alpha fetoprotein (AFP) and microvessel density and a better tumor differentiation. Multivariate analyses revealed that reduced RECK expression was an independent prognostic factor for recurrence and poor prognosis. In conclusion, high RECK expression reflects an immunogenic and hypovascularity status in HCC. RECK is a promising prognostic marker for survival of HCC and may act as a complementary indicator for patients to receive anti-angiogenic therapy or immunotherapy.
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http://dx.doi.org/10.7150/jca.56167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176247PMC
May 2021

Animal naming test for the assessment of minimal hepatic encephalopathy in Asian cirrhotic populations.

Clin Res Hepatol Gastroenterol 2021 Jun 6;45(5):101729. Epub 2021 Jun 6.

Department of Infectious Disease and Hepatology, The Second Hospital, Cheeloo College of Medicine, Shandong University, China. Electronic address:

Background And Aim: To evaluate the diagnostic performance of simplified animal naming test (S-ANT1) for minimal hepatic encephalopathy (MHE) in patients with cirrhosis from a Chinese tertiary centre and to optimize the application strategy of S-ANT1 in clinical practice.

Methods: The Animal Naming Test 1 (ANT1) was performed in all included cirrhotic patients and healthy volunteers. S-ANT1 was calculated to adjust for age and education. Psychometric Hepatic Encephalopathy Score (PHES) was also performed in patients with cirrhosis.

Results: 88 cirrhotic patients and 34 healthy control subjects were included. Cirrhotic patients were characterized with lower S-ANT1 scores (P =  0.001). In patients with cirrhosis, score of S-ANT1 was correlated with PHES score, age, school education period, and blood ammonia (all P values <0.05). With ≤20 animals as the cut-off value, S-ANT1 could distinguish MHE and no MHE with a sensitivity of 77.5% and a specificity of 58.3%. A three-step screening strategy, with 90% as a threshold for sensitivity and specificity and two cut-off values "≤12 animals" and ">23 animals", was then formulated to rule out patients with high possibility of MHE and with high possibility of no MHE. The remaining "ruled-in" patients should be further evaluated for MHE using PHES.

Conclusions: S-ANT1 is an important screening tool for MHE in cirrhotic patients. The three-step screening strategy based on S-ANT1 and PHES is conducive to the identification of MHE in clinical practice.
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http://dx.doi.org/10.1016/j.clinre.2021.101729DOI Listing
June 2021

Prenatal prediction and typing of placental invasion using MRI deep and radiomic features.

Biomed Eng Online 2021 Jun 5;20(1):56. Epub 2021 Jun 5.

Faculty of Electrical Engineering and Computer Science, Ningbo University, Ningbo, 315211, Zhejiang, China.

Background: To predict placental invasion (PI) and determine the subtype according to the degree of implantation, and to help physicians develop appropriate therapeutic measures, a prenatal prediction and typing of placental invasion method using MRI deep and radiomic features were proposed.

Methods: The placental tissue of abdominal magnetic resonance (MR) image was segmented to form the regions of interest (ROI) using U-net. The radiomic features were subsequently extracted from ROI. Simultaneously, a deep dynamic convolution neural network (DDCNN) with codec structure was established, which was trained by an autoencoder model to extract the deep features from ROI. Finally, combining the radiomic features and deep features, a classifier based on the multi-layer perceptron model was designed. The classifier was trained to predict prenatal placental invasion as well as determine the invasion subtype.

Results: The experimental results show that the average accuracy, sensitivity, and specificity of the proposed method are 0.877, 0.857, and 0.954 respectively, and the area under the ROC curve (AUC) is 0.904, which outperforms the traditional radiomic based auxiliary diagnostic methods.

Conclusions: This work not only labeled the placental tissue of MR image in pregnant women automatically but also realized the objective evaluation of placental invasion, thus providing a new approach for the prenatal diagnosis of placental invasion.
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http://dx.doi.org/10.1186/s12938-021-00893-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180077PMC
June 2021

Operational research capacity building through the Structured Operational Research Training Initiative (SORT-IT) in China: implementation, outcomes and challenges.

Infect Dis Poverty 2021 Jun 1;10(1):80. Epub 2021 Jun 1.

Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Beijing, China.

Background: Chinese Center for Disease Control and Prevention (China CDC) introduced the Structured Operational Research Training Initiative (SORT IT) into China to build a special capacity and equip public health professionals with an effective tool to support developing countries in strengthening their operational research. The paper aims to investigate and analyze the implementation, outcomes and challenges of the first cycle of SORT IT in China.

Main Text: As a result of the successful implementation, SORT IT China, Cycle 1 has demonstrated fruitful outputs as exemplified by the 18-month follow-up to the post-training initiatives of the twelve participants, who all achieved the four milestones required by SORT IT. Eleven of twelve (92%) manuscripts generated that focused on the prevention and control of malaria, influenza, HIV/AIDS, hepatitis B, schistosomiasis, tuberculosis and Japanese encephalitis were published by peer-reviewed international journals with the impact factor ranging from 2.6 to 4.8. The most up-to-date citation count on February 19, 2021 was 53 times out of which 31 times were cited by Science Citation Index papers with 94.827 impact factor in total. Six senior professionals from China CDC also facilitated the whole SORT IT training scheme as co-mentors under the guidance of SORT IT mentors. The twelve participants who gained familiarity with the SORT IT courses and training principles are likely become potential mentors for future SORT IT, but they as the non-first language speakers/users of English also faced the challenge in thoroughly understanding the modules delivered in English and writing English academically to draft the manuscripts.

Conclusion: The outcomes from the first cycle of SORT IT in China have led to studies contributing to narrowing the knowledge gap among numerous public health challenges nationally and internationally. It is believed the researchers who participated will continue to apply the skills learned within their domain and help build the training capacity for future operational research courses both in China and in developing countries with similar needs.
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http://dx.doi.org/10.1186/s40249-021-00865-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170796PMC
June 2021

Two Different Inoculation Methods Unveiled the Relative Independence of DON Accumulation in Wheat Kernels from Disease Severity on Spike after Infection by Fusarium Head Blight.

Toxins (Basel) 2021 05 14;13(5). Epub 2021 May 14.

Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Collaborative-Innovation of Modern Crops and Food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou University, Yangzhou 225009, China.

Fusarium head blight (FHB) causes wheat yield loss and mycotoxin (deoxynivalenol, DON) accumulation in wheat kernel. Developing wheat cultivars with overall resistance to both FHB spread within a spike and DON accumulation in kernels is crucial for ensuring food security and food safety. Here, two relatively novel inoculation methods, bilateral floret inoculation (BFI) and basal rachis internode injection (BRII), were simultaneously employed to evaluate disease severity and DON content in kernels in a segregating population of recombinant inbred lines (RILs) developed from Ning 7840 (carrying ) and Clark (without ). Under both inoculation methods, four contrasting combinations of disease severity and DON content were identified: high severity/high DON (HSHD), high severity/low DON (HSLD), low severity/high DON (LSHD) and low severity/low DON (LSLD). Unexpectedly, the BRII method clearly indicated that disease severity was not necessarily relevant to DON concentration. The effects of on disease severity, and on DON concentrations, agreed very well across the two methods. Several lines carrying showed extremely higher severity and (or) DON content under both inoculation methods. The "Mahalanobis distance" (MD) method was used to rate overall resistance of a line by inclusion of both disease severity and DON content over both methods to select LSLD lines.
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http://dx.doi.org/10.3390/toxins13050353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156083PMC
May 2021

A Heterozygous Genotype-Dependent Branched-Spike Wheat and the Potential Genetic Mechanism Revealed by Transcriptome Sequencing.

Biology (Basel) 2021 May 14;10(5). Epub 2021 May 14.

Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Collaborative Innovation of Modern Crops and Food Crops in Jiangsu, Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou University, Yangzhou 225009, China.

Wheat ( L.) spike architecture is an important trait associated with spike development and grain yield. Here, we report a naturally occurring wheat mutant with branched spikelets (BSL) from its wild-type YD-16, which has a normal spike trait and confers a moderate level of resistance to wheat Fusarium head blight (FHB). The lateral meristems positioned at the basal parts of the rachis node of the BSL mutant develop into ramified spikelets characterized as multiple spikelets. The BSL mutant shows three to four-day longer growth period but less 1000-grain weight than the wild type, and it becomes highly susceptible to FHB infection, indicating that the locus controlling the BSL trait may have undergone an intensively artificial and/or natural selection in modern breeding process. The self-pollinated descendants of the lines with the BSL trait consistently segregated with an equal ratio of branched and normal spikelets (NSL) wheat, and homozygotes with the BSL trait could not be achieved even after nine cycles of self-pollination. Distinct segregation patterns both from the self-pollinated progenies of the BSL plants and from the reciprocal crosses between the BSL plants with their sister NSL plants suggested that gametophytic male sterility was probably associated with the heterozygosity for the BSL trait. Transcriptome sequencing of the RNA bulks contrasting in the two types of spike trait at the heading stage indicated that the genes on chromosome 2D may be critical for the BSL trait formation since 329 out of 2540 differentially expressed genes (DEGs) were located on that chromosome, and most of them were down-regulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that carbohydrate metabolism may be involved in the BSL trait expression. This work provides valuable clues into understanding development and domestication of wheat spike as well as the association of the BSL trait with FHB susceptibility.
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http://dx.doi.org/10.3390/biology10050437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157103PMC
May 2021

Antifungal Activity of Quinofumelin against and Its Inhibitory Effect on DON Biosynthesis.

Toxins (Basel) 2021 05 12;13(5). Epub 2021 May 12.

Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

causal agent of Fusarium head blight (FHB), causes a huge economic loss. No information is available on the activity of quinofumelin, a novel quinoline fungicide, against or other phytopathogens. In this study, we used mycelial growth and spore germination inhibition methods to determine the inhibitory effect of quinofumelin against in vitro. The results indicated that quinofumelin excellently inhibited mycelial growth and spore germination of , with the average EC values of 0.019 ± 0.007 μg/mL and 0.087 ± 0.024 μg/mL, respectively. In addition, we found that quinofumelin could significantly decrease deoxynivalenol (DON) production and inhibit the expression of DON-related gene in . Furthermore, we found that quinofumelin could disrupt the formation of Fusarium toxisome, a structure for producing DON. Western blot analysis demonstrated that the translation level of TRI1, a marker gene for Fusarium toxisome, was suppressed by quinofumelin. The protective and curative assays indicated that quinofumelin had an excellent control efficiency against on wheat coleoptiles. Taken together, quinofumelin exhibits not only an excellent antifungal activity on mycelial growth and spore germination, but also could inhibit DON biosynthesis in . The findings provide a novel candidate for controlling FHB caused by .
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http://dx.doi.org/10.3390/toxins13050348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151098PMC
May 2021

Influences of modified biochar on metal bioavailability, metal uptake by wheat seedlings (Triticum aestivum L.) and the soil bacterial community.

Ecotoxicol Environ Saf 2021 Sep 28;220:112370. Epub 2021 May 28.

College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha 410007, China. Electronic address:

A 6 weeks pot culture experiment was carried out to investigate the stabilization effects of a modified biochar (BCM) on metals in contaminated soil and the uptake of these metals by wheat seedlings. The results showed that the application of BCM significantly increased the soil fertility, the biomass of wheat seedling roots increased by more than 50%, and soil dehydrogenase (DHA) and catalase (CAT) activities increased by 369.23% and 12.61%, respectively. In addition, with the application of BCM, the diethylenetriaminepentaacetic acid extractable (DTPA-extractable) Cd, Pb, Cu and Zn in soil were reduced from 2.34 to 0.38 mg/kg, from 49.27 to 25.65 mg/kg, from 3.55 mg/kg to below the detection limit and from 4.05 to 3.55 mg/kg, respectively. Correspondingly, the uptake of these metals in wheat roots and shoots decreased by 62.43% and 79.83% for Cd, 73.21% and 66.32% for Pb, 57.98% and 68.92% for Cu, and 40.42% and 43.66% for Zn. Furthermore, BCM application decreased the abundance and alpha diversity of soil bacteria and changed the soil bacterial community structure dramatically. Overall, BCM has great potential for the remediation of metal-contaminated soils, but its long-term impact on soil metals and biota need further research.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112370DOI Listing
September 2021

Enhanced Electroluminescence Based on a -Conjugated Heptazine Derivative by Exploiting Thermally Activated Delayed Fluorescence.

Front Chem 2021 13;9:693813. Epub 2021 May 13.

College of Optoelectronic Technology, Chengdu University of Information Technology, Chengdu, China.

Heptazine derivatives have attracted much attention over the past decade by virtue of intriguing optical, photocatalytic as well as electronic properties in the fields of hydrogen evolution, organic optoelectronic technologies and so forth. Here, we report a simple -conjugated heptazine derivative (HAP-3DF) possessing an n→* transition character which exhibits enhanced electroluminescence by exploiting thermally activated delayed fluorescence (TADF). Green-emitting HAP-3DF shows relatively low photoluminescence quantum efficiencies () of 0.08 in toluene and 0.16 in doped film with bis(2-(diphenylphosphino)phenyl) ether oxide (DPEPO) as the matrix. Interestingly, the organic light-emitting diode (OLED) incorporating 8 wt% HAP-3DF:DPEPO as an emitting layer achieved a high external quantum efficiency (EQE) of 3.0% in view of the fairly low of 0.16, indicating the presence of TADF stemming from n→* transitions. As the matrix changing from DPEPO to 1,3-di (9-carbazol-9-yl)benzene (mCP), a much higher of 0.56 was found in doped film accompanying yellow emission. More importantly, enhanced electroluminescence was observed from the OLED containing 8 wt% HAP-3DF:mCP as an emitting layer, and a rather high EQE of 10.8% along with a low roll-off was realized, which should be ascribed to the TADF process deriving from exciplex formation.
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http://dx.doi.org/10.3389/fchem.2021.693813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155250PMC
May 2021

A Novel Cross-Linked Hemoglobin-Based Oxygen Carrier, YQ23, Extended the Golden Hour for Uncontrolled Hemorrhagic Shock in Rats and Miniature Pigs.

Front Pharmacol 2021 12;12:652716. Epub 2021 May 12.

State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Daping Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Hypotensive resuscitation is widely applied for trauma and war injury to reduce bleeding during damage-control resuscitation, but the treatment time window is limited in order to avoid hypoxia-associated organ injury. Whether a novel hemoglobin-based oxygen carrier (HBOC), YQ23 in this study, could protect organ function, and extend the Golden Hour for treatment is unclear. Uncontrolled hemorrhagic shock rats and miniature pigs were infused with 0.5, 2, and 5% YQ23 before bleeding was controlled, while Lactate Ringer's solution (LR) and fresh whole blood plus LR (WB + LR) were set as controls. During hypotensive resuscitation the mean blood pressure was maintained at 50-60 mmHg for 60 min. Hemodynamics, oxygen delivery and utilization, blood loss, fluid demand, organ function, animal survival as well as side effects were observed. Besides, in order to observe whether YQ23 could extend the Golden Hour, the hypotensive resuscitation duration was extended to 180 min and animal survival was observed. Compared with LR, infusion of YQ23 in the 60 min pre-hospital hypotensive resuscitation significantly reduced blood loss and the fluid demand in both rats and pigs. Besides, YQ23 could effectively stabilize hemodynamics, and increase tissue oxygen consumption, increase the cardiac output, reduce liver and kidney injury, which helped to reduce the early death and improve animal survival. In addition, the hypotensive resuscitation duration could be extended to 180 min using YQ23. Side effects such as vasoconstriction and renal injury were not observed. The beneficial effects of 5% YQ23 are equivalent to similar volume of WB + LR. HBOC, such as YQ23, played vital roles in damage-control resuscitation for emergency care and benefited the uncontrolled hemorrhagic shock in the pre-hospital treatment by increasing oxygen delivery, reducing organ injury. Besides, HBOC could benefit the injured and trauma patients by extending the Golden Hour.
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http://dx.doi.org/10.3389/fphar.2021.652716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149754PMC
May 2021

Spinal-Shortening Process Positively Improves Associated Syringomyelia in Patients with Scoliosis After Single-Stage Spinal Correction.

World Neurosurg 2021 May 27. Epub 2021 May 27.

Department of Orthopaedics, The Second Affiliated Hospital of Kunming Medical University, Kunming, People's Republic of China.

Objective: Single-stage spinal correction without previous neurosurgical intervention has been attempted in patients with scoliosis associated with syringomyelia (SM). However, evidence to demonstrate its potential influence on associated SM from direct spinal correction is still lacking. The aim of the present study was to explore the role of spinal shortening in the prognosis of SM-associated scoliosis after single-stage spinal correction without previous neurosurgical intervention.

Methods: Patients with SM-associated scoliosis without previous neurological intervention, who had undergone posterior direct instrumental correction (PDIC) without osteotomy and posterior vertebral column resection (PVCR) at a single center, were selected for comparative analysis. The basic demographic and pre- and postoperative imaging data of the spinal deformity and SM at the final follow-up were compared separately for the 2 different spinal correction procedures.

Results: A total of 23 patients were included in the final analysis: 13 had undergone PDIC and 10 had undergone PVCR. The mean follow-up period was 6.2 years (range, 5-9 years). At the final follow-up, the mean corrective rate of scoliosis and kyphosis was 65.7% and 48.4%, respectively. Obvious SM reduction was achieved in 11 patients (47.8%), with an average reduction of 37.3%. No patient experienced neurologic deterioration or had required further neurosurgical intervention for SM during follow-up. The patients who had undergone PVCR had had much more severe scoliosis (98.8° vs. 60.5°; P = 0.000) and kyphosis (74.8° vs. 43.6°; P = 0.032). Moreover, 80.0% of the patients who had undergone PVCR had experienced obvious SM improvement compared with 23.1% of those who had undergone PDIC (P = 0.007).

Conclusions: The reduction of spinal cord tension is an important factor influencing SM improvement. As the most powerful spinal-shortening osteotomy, PVCR can effectively correct severe spinal deformities and improve associated SM. Single-stage posterior spinal correction can be a potential choice for selected patients with scoliosis and untreated SM using strict inclusion criteria, which will not only achieve safe spinal correction but could also steadily improve and stabilize SM.
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http://dx.doi.org/10.1016/j.wneu.2021.05.073DOI Listing
May 2021

Platelet-Rich Plasma Has Better Results for Retear Rate, Pain, and Outcome Than Platelet-Rich Fibrin After Rotator Cuff Repair: A Systematic Review and Meta-analysis of Randomized Controlled Trials.

Arthroscopy 2021 May 27. Epub 2021 May 27.

Department of Orthopaedic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.

Purpose: To perform a systematic review and meta-analysis of randomized controlled trials (RCTs) in the literature to ascertain the extent to which platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) improved patient outcomes in arthroscopic rotator cuff repair.

Methods: Two independent reviewers performed the literature search based on the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines, with a third author resolving any discrepancies. RCTs comparing PRP or PRF to a control in rotator cuff repair were included. Quality of evidence was assessed using the Cochrane Collaboration risk of bias tool. Clinical outcomes were compared using the risk ratio for dichotomous variables and the mean difference for continuous variables. A P value <.05 was deemed statistically significant.

Results: Included in this review are 23 RCTs with 1440 patients. PRP resulted in significantly decreased rates of retear (15.9% versus 29.0%, respectively; P < .0001). Significant results were noted in favor of PRP compared with control based on the Constant score (83.9 versus 81.2, respectively; P = .0006); the University of California, Los Angeles score (31.1 versus 30.2; P < .00001); the American Shoulder and Elbow Surgeons score (87.3 versus 84.5; P = .04); and the visual analog scale score (1.3 versus 1.6; P = .01). PRF resulted in an improved Constant score (80.1 versus 80.0, respectively; P = .04) compared with control.

Conclusions: The current evidence shows that using PRP in arthroscopic rotator cuff repair can improve pain levels and functional outcome scores while reducing the retear rate after surgery. PRF injection, on the other hand, improves only the Constant score.

Level Of Evidence: II; systematic review and meta-analysis of level I and II evidence.
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http://dx.doi.org/10.1016/j.arthro.2021.05.023DOI Listing
May 2021

Differential responses of 23 maize cultivar seedlings to an arbuscular mycorrhizal fungus when grown in a metal-polluted soil.

Sci Total Environ 2021 May 25;789:148015. Epub 2021 May 25.

State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Yunnan University, Kunming 650091, PR China. Electronic address:

Modern breeding efforts have been accelerating crop improvement and yielding numerous cultivars with distinct genetic traits; however, interactions between different cultivars and their root-associated arbuscular mycorrhizal fungi (AMF) are not clear. Herein, we selected the 22 most common commercial maize (Zea mays) varieties in China and an inbred line (B73) to study the differential responses of these 23 cultivars to mycorrhizal inoculation when grown in an arable soil polluted by multiple metals (Pb, Zn, and Cd). We found that the different cultivars exhibited significant variations in plant metal accumulation, ranging from strong metal exclusion (ZYY9) to strong metal accumulation (B73). Mycorrhizal colonization substantially altered metal uptake and repartitioning, while bioaugmenting the inherent characteristics of metal accumulation; for example, the AMF enhanced leaf accumulation of the metal-accumulator B73, and markedly reduced the root uptake of the metal-excluder ZYY9. However, such AMF-induced alterations were also substantially dependent on plant organs (roots and shoots) and metal species. We found that the extent of the AMF-induced leaf alterations was substantially greater than that of the root alterations. Similarly, the number of instances where the AMF significantly altered the Zn and Cd accumulation was far higher than the number of instances where Pb accumulation was significantly altered by AMF. In addition, the presence of AMF appeared to trigger the maize antioxidant systems, which may have alleviated the toxicity of excessive Cd, increased the leaf chlorophyll content, augmented the net photosynthetic rate, and promoted the growth of 17.39% of the maize cultivars. Our results suggest that a future crop breeding challenge is to produce cultivars for safe production or phytoremediation, thereby optimizing the combinations of crop cultivars and their root-associated AMF in slightly to moderately metal-polluted arable soils.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148015DOI Listing
May 2021

The role of AQP3 in the vascular leakage of sepsis and the protective effect of Ss-31.

J Cardiovasc Pharmacol 2021 May 27. Epub 2021 May 27.

State Key Laboratory of Trauma, Burns and Combined Injury, Shock and Transfusion Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, People's Republic of China.

Abstract: Aquaporins(AQPs) are a group of membrane proteins related to water permeability. Studies have shown AQPs play a vital role in various diseases. Whether AQPs participate in regulating vascular permeability after sepsis and the subtype of AQPs is related are unknown. Ss-31, as a new antioxidant, had protective effects on a variety of diseases. However, whether Ss-31 has a protective effect on pulmonary vascular permeability in sepsis and whether its effect is related to AQPs is unclear. Using the cecum ligation puncture (CLP) induced septic rat and LPS treated pulmonary vein endothelial cells(PVECs), the role of AQPs in the regulation of the permeability of pulmonary vascular, and its relationship to Ss-31 were studied. The results showed that the pulmonary vascular permeability significantly increased after sepsis, meanwhile the expressions of AQP3, 4, and 12 increased. Among those, the AQP3 was closely correlated with pulmonary vascular permeability. Inhibition of AQP3 antagonized the increase of the permeability of monolayer PVECs. Further study showed that the expression of Caveolin-1(Cav-1) increased and Occludin decreased after sepsis. Inhibition of AQP3 antagonized the decrease of Cav-1 and the increase of Occludin in sepsis. Antioxidant Ss-31 decreased the expression of AQP3 and ROS levels. At the same time, Ss-31 improved pulmonary vascular permeability and prolonged survival of sepsis rats. In conclusion, AQP3 participates in the regulation of pulmonary vascular permeability after sepsis, and the antioxidant Ss-31 has a protective effect on pulmonary vascular permeability by down-regulating the expression of AQP3 and inhibiting ROS production.
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http://dx.doi.org/10.1097/FJC.0000000000001050DOI Listing
May 2021

Ultralong room-temperature phosphorescence remarkably weakened by halogenation-induced molecular packing in hexaphenylmelamine derivatives.

Chem Commun (Camb) 2021 May 28. Epub 2021 May 28.

College of Optoelectronic Engineering, Chengdu University of Information Technology, Chengdu 610225, China.

A series of pure organic halogenated hexaphenylmelamine (HPM) derivatives featuring remarkably weakened ultralong room-temperature phosphorescence (RTP) were meticulously investigated. As the p-substituted atoms of these HPM derivatives sequentially changed from H to F, Cl and Br, both the RTP lifetimes and efficiencies dramatically decreased from 608 ms with 13.4% (HPM-H) to 337 ms with 5.3% (HPM-F), 99 ms with 1.3% (HPM-Cl), and 2.8 ms with undetectable efficiency (HPM-Br), respectively. Most notably, the severely weakened efficiencies are fundamentally different from the trends of the effect of halogenation on phosphorescence properties previously reported. Coupled with experimental results and theoretical simulations, the subtle change of molecular packing induced by halogenation should be responsible for the distinctive RTP properties. This finding not only provides a unique halogen-involved RTP phenomenon, but also offers a very special perspective to understand the effect of halogenation on phosphorescence.
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http://dx.doi.org/10.1039/d1cc01533hDOI Listing
May 2021

3D bio-printed biphasic scaffolds with dual modification of silk fibroin for the integrated repair of osteochondral defects.

Biomater Sci 2021 May 28. Epub 2021 May 28.

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, China. and Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, No. 1956 Huashan Road, Shanghai, 200030, China.

Repair of osteochondral defects is still a challenge, especially the regeneration of hyaline cartilage. Parathyroid hormone (PTH) can inhibit the hypertrophy of chondrocytes to maintain the phenotype of hyaline cartilage. Here, we aimed to construct a bio-printed biphasic scaffold with a mechanical gradient based on dual modification of silk fibroin (SF) for the integrated repair of osteochondral defects. Briefly, SF was grafted with PTH (SF-PTH) and covalently immobilized with methacrylic anhydride (SF-MA), respectively. Next, gelatin methacryloyl (GM) mixed with SF-PTH or SF-MA was used as a bio-ink for articular cartilage and subchondral bone regeneration. Finally, the GM + SF-PTH/GM + SF-MA osteochondral biphasic scaffold was constructed using 3D bioprinting technology, and implanted in a rabbit osteochondral defect model. In this study, the SF-PTH bio-ink was synthesized for the first time. In vitro results indicated that the GM + SF-MA bio-ink had good mechanical properties, while the GM + SF-PTH bio-ink inhibited the hypertrophy of chondrocytes and was beneficial for the production of hyaline cartilage extracellular matrix. Importantly, an integrated GM + SF-PTH/GM + SF-MA biphasic scaffold with a mechanical gradient was successfully constructed. The results in vivo demonstrated that the GM + SF-PTH/GM + SF-MA scaffold could promote the regeneration of osteochondral defects and maintain the phenotype of hyaline cartilage to a large extent. Collectively, our results indicate that the integrated GM + SF-PTH/GM + SF-MA biphasic scaffold constructed by 3D bioprinting is expected to become a new strategy for the treatment of osteochondral defects.
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http://dx.doi.org/10.1039/d1bm00535aDOI Listing
May 2021