Publications by authors named "Tao Jin"

756 Publications

Mass-manufactured beam-steering metasurfaces for high-speed full-duplex optical wireless broadcasting communications.

Adv Mater 2021 Nov 26:e2106080. Epub 2021 Nov 26.

State Key Laboratory of Optical Communication Technologies and Networks, China Information Communication Technologies Group Corporation (CICT), Wuhan, 430074, China.

Beam-steering devices, which are at the heart of optical wireless broadcasting communication links, play an important role in data allocation and exchange. An ideal beam-steering device features large steering angles, arbitrary channel numbers, reconfigurability, and ultra-compactness. However, these criteria have been achieved only partially with conventional beam-steering devices based on waveguides, micro-electrical mechanical systems, spatial light modulators, and gratings, which would substantially limit the application of optical wireless broadcasting communication techniques. In this study, we designed and experimentally demonstrated an ultra-compact full-duplex meta-broadcasting communication system, which exhibits beam steering angles up to ±40°, 14 broadcasting channels with capacity for downstream and upstream links up to 100 and 10 Gbps for each user channel, three operating modes for flexible signal switching, and meta-device dimensions as small as 2 mm × 2 mm. In particular, the beam-steering meta-devices are mass-manufactured by a complementary metal-oxide-semiconductor processing platform, which shows their potential for large-scale commercial applications. The demonstrated meta-broadcasting communication system merges optical wireless broadcasting communications and metasurfaces, which reduces the complexity of beam-steering devices while significantly increasing their performance, opening up a new avenue for high-quality optical wireless broadcasting communications. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202106080DOI Listing
November 2021

Composition and Dynamics of H1N1 and H7N9 Influenza A Virus Quasispecies in a Co-infected Patient Analyzed by Single Molecule Sequencing Technology.

Front Genet 2021 3;12:754445. Epub 2021 Nov 3.

BGI-Qingdao, BGI-Shenzhen, Qingdao, China.

A human co-infected with H1N1 and H7N9 subtypes influenza A virus (IAV) causes a complex infectious disease. The identification of molecular-level variations in composition and dynamics of IAV quasispecies will help to understand the pathogenesis and provide guidance for precision medicine treatment. In this study, using single-molecule real-time sequencing (SMRT) technology, we successfully acquired full-length IAV genomic sequences and quantified their genotypes abundance in serial samples from an 81-year-old male co-infected with H1N1 and H7N9 subtypes IAV. A total of 26 high diversity nucleotide loci was detected, in which the A-G base transversion was the most abundant substitution type (67 and 64%, in H1N1 and H7N9, respectively). Seven significant amino acid variations were detected, such as NA:H275Y and HA: R222K in H1N1 as well as PB2:E627K and NA: K432E in H7N9, which are related to viral drug-resistance or mammalian adaptation. Furtherly, we retrieved 25 H1N1 and 22 H7N9 genomic segment haplotypes from the eight samples based on combining high-diversity nucleotide loci, which provided a more concise overview of viral quasispecies composition and dynamics. Our approach promotes the popularization of viral quasispecies analysis in a complex infectious disease, which will boost the understanding of viral infections, pathogenesis, evolution, and precision medicine.
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http://dx.doi.org/10.3389/fgene.2021.754445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595946PMC
November 2021

Identification of cold tolerance QTLs at the bud burst stage in 211 rice landraces by GWAS.

BMC Plant Biol 2021 Nov 20;21(1):542. Epub 2021 Nov 20.

Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Nanchang, 330045, Jiangxi Province, China.

Background: Rice is a crop that is very sensitive to low temperature, and its morphological development and production are greatly affected by low temperature. Therefore, understanding the genetic basis of cold tolerance in rice is of great significance for mining favorable genes and cultivating excellent rice varieties. However, there have been limited studies focusing on cold tolerance at the bud burst stage; therefore, considerable attention should be given to the genetic basis of cold tolerance at this stage.

Results: In this study, a natural population consisting of 211 rice landraces collected from 15 provinces in China and other countries was used for the first time to evaluate cold tolerance at the bud burst stage. Population structure analysis showed that this population was divided into two groups and was rich in genetic diversity. Our evaluation results confirmed that japonica rice was more tolerant to cold at the bud burst stage than indica rice. A genome-wide association study (GWAS) was performed with the phenotypic data of 211 rice landraces and a 36,727 SNP dataset under a mixed linear model. Twelve QTLs (P < 0.0001) were identified for the seedling survival rate (SR) after treatment at 4 °C, in which there were five QTLs (qSR2-2, qSR3-1, qSR3-2, qSR3-3 and qSR9) that were colocalized with those from previous studies and seven QTLs (qSR2-1, qSR3-4, qSR3-5, qSR3-6, qSR3-7, qSR4 and qSR7) that were reported for the first time. Among these QTLs, qSR9, harboring the most significant SNP, explained the most phenotypic variation. Through bioinformatics analysis, five genes (LOC_Os09g12440, LOC_Os09g12470, LOC_Os09g12520, LOC_Os09g12580 and LOC_Os09g12720) were identified as candidates for qSR9.

Conclusion: This natural population consisting of 211 rice landraces combined with high-density SNPs will serve as a better choice for identifying rice QTLs/genes in the future, and the detected QTLs associated with cold tolerance at the bud burst stage in rice will be conducive to further mining favorable genes and breeding rice varieties under cold stress.
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http://dx.doi.org/10.1186/s12870-021-03317-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605578PMC
November 2021

Wearable Triboelectric Sensors Enabled Gait Analysis and Waist Motion Capture for IoT-Based Smart Healthcare Applications.

Adv Sci (Weinh) 2021 Nov 19:e2103694. Epub 2021 Nov 19.

Department of Electrical and Computer Engineering, National University of Singapore, 4 Engineering Drive 3, Singapore, 117583, Singapore.

Gait and waist motions always contain massive personnel information and it is feasible to extract these data via wearable electronics for identification and healthcare based on the Internet of Things (IoT). There also remains a demand to develop a cost-effective human-machine interface to enhance the immersion during the long-term rehabilitation. Meanwhile, triboelectric nanogenerator (TENG) revealing its merits in both wearable electronics and IoT tends to be a possible solution. Herein, the authors present wearable TENG-based devices for gait analysis and waist motion capture to enhance the intelligence and performance of the lower-limb and waist rehabilitation. Four triboelectric sensors are equidistantly sewed onto a fabric belt to recognize the waist motion, enabling the real-time robotic manipulation and virtual game for immersion-enhanced waist training. The insole equipped with two TENG sensors is designed for walking status detection and a 98.4% identification accuracy for five different humans aiming at rehabilitation plan selection is achieved by leveraging machine learning technology to further analyze the signals. Through a lower-limb rehabilitation robot, the authors demonstrate that the sensory system performs well in user recognition, motion monitoring, as well as robot and gaming-aided training, showing its potential in IoT-based smart healthcare applications.
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http://dx.doi.org/10.1002/advs.202103694DOI Listing
November 2021

Evaluating Incidence, Location, and Predictors of Positive Surgical Margin Among Chinese Men Undergoing Robot-Assisted Radical Prostatectomy.

Cancer Control 2021 Jan-Dec;28:10732748211055265

Department of Urology, The First Affiliated Hospital of 191599Zhengzhou University, Zhengzhou, China.

Purpose: To evaluate the incidence and locations of positive surgical margin (PSM) among Chinese men undergoing RARP and identify the preoperative predictors for PSM.

Methods: We retrospectively identified 393 patients who underwent RARP according to inclusion criteria by single surgeon in our hospital. PSM was defined as the presence of cancer adjacent to inked surface of the specimen and categorized into four groups based on locations: apex, posterolateral, base, and multifocal. Logistic regression analysis was performed to identify the predictors of overall and location-specific PSM.

Results: The overall PSM rate was 133/393 (34%). The PSM rates for pT2, pT3, and pT4 stage were 63/278 (23%), 50/89 (56%), and 20/26 (77%), respectively. The estimated rates for apical, posterolateral, basal, and multifocal PSM were 8%, 4%, 7%, and 14%, respectively. In univariate analysis, overall PSM related to tPSA, f/tPSA, percentage of positive needles, and Gleason score. Multifocal PSM correlated with smoking history, drinking history, tPSA, f/tPSA, percentage of positive needles, and Gleason score. In multivariate analysis, percentage of positive needles reminded the only independent predictor for overall (OR = 10.5, 95% CI: 2.58-44.4) and basal PSM (OR = 24.0, 95% CI: 3.22-179.4). The f/tPSA (OR = 2.59, 95% CI: 2.18-5.71) and percentage of positive needles (OR = 31.0, 95% CI: 3.17-303) were independent risk factors for multifocal PSM.

Conclusion: The multifocal sites were the most common location of positive surgical margin, followed by apical and basal sites among Chinese patients undergoing RARP. The percentage of positive needles was an independent predictor for overall, basal, and multifocal PSM.
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http://dx.doi.org/10.1177/10732748211055265DOI Listing
November 2021

Role of Toll-Like Receptors in Neuroimmune Diseases: Therapeutic Targets and Problems.

Front Immunol 2021 1;12:777606. Epub 2021 Nov 1.

Department of Neurology and Neuroscience Center, The First Hospital of Jilin University, Changchun, China.

Toll-like receptors (TLRs) are a class of proteins playing a key role in innate and adaptive immune responses. TLRs are involved in the development and progression of neuroimmune diseases initiating inflammatory responses. Thus, targeting TLRs signaling pathway may be considered as a potential therapy for neuroimmune diseases. However, the role of TLRs is elusive and complex in neuroimmune diseases. In addition to the inadequate immune response of TLRs inhibitors in the experiments, the recent studies also demonstrated that partial activation of TLRs is conducive to the production of anti-inflammatory factors and nervous system repair. Exploring the mechanism of TLRs in neuroimmune diseases and combining with developing the emerging drug may conquer neuroimmune diseases in the future. Herein, we provide an overview of the role of TLRs in several neuroimmune diseases, including multiple sclerosis, neuromyelitis optica spectrum disorder, Guillain-Barré syndrome and myasthenia gravis. Emerging difficulties and potential solutions in clinical application of TLRs inhibitors will also be discussed.
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http://dx.doi.org/10.3389/fimmu.2021.777606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591135PMC
November 2021

Overexpressed GNA13 induces temozolomide sensitization via down-regulating MGMT and p-RELA in glioma.

Am J Transl Res 2021 15;13(10):11413-11426. Epub 2021 Oct 15.

Department of Neurosurgery, Shantou Central Hospital Shantou 515041, China.

Temozolomide (TMZ), one of the few effective drugs used during adjuvant therapy, could effectively prolong the overall survival (OS) of glioma patients. In our previous study, the mRNA level of G Protein Subunit Alpha 13 (GNA13) was found to be inversely correlated with OS and was therefore identified as a potential biomarker for the prognosis of glioma. Henceforth, this study aims to identify the molecular mechanism of GNA13 in enhancing TMZ sensitization through bioinformatic analyses of GSE80729 and GSE43452 and other experiments. In glioma, overexpression of GNA13 downregulated PRKACA, which is a subunit of PKA, hence reducing phosphorylated RELA and MGMT. Since p-RELA and MGMT were proven to be closely associated with TMZ resistance, we therefore investigated whether thetwo signaling pathways, "GNA13/PRKACA/p-RELA", and "GNA13/PRKACA/MGMT", were involved in the molecular mechanism of GNA13 in TMZ sensitization. Our conclusion was that, GNA13 overexpression in glioma cells were more sensitive in TMZ treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581860PMC
October 2021

Therapeutic effects of cationic liposomes on lupus-prone MRL/lpr mice are mediated via inhibition of TLR4-triggered B-cell activation.

Nanomedicine 2021 Nov 12:102491. Epub 2021 Nov 12.

School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China; College of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, Zhejiang 315100, China. Electronic address:

We previously reported that co-delivery of dihydroartemisinin and high mobility group box 1 (HMGB1) siRNAs, using cell penetrating peptide (TAT)-modified cationic liposomes (TAT-CLs-DHA/siRNA), resulted in promising activity for the treatment of inflammatory disease through TLR4 signaling pathway. In the current study, we further investigated the therapeutic effects of TAT-CLs-DHA/siRNA on lupus-prone MRL/lpr mice and explored its effects on B cell responses. In vitro, we found that TAT-CLs-DHA/siRNA suppressed the proliferation and activation of B cells through the TLR4 signaling pathway. Following parenteral administration every 4days, TAT-CLs-DHA/siRNA significantly reduced proteinuria, glomerulonephritis, serum anti-dsDNA antibody and secretion of interleukin (IL)-6, IL-10, IL-17 and IL-21. Moreover, Western blotting showed that TAT-CLs-DHA/siRNA modulated the B-cell intrinsic pathway by downregulating expression of HMGB1, TLR4, MyD88 and NF-κB. This co-delivery system thus represents a promising treatment option for lupus nephritis, and also highlights a novel target of lupus treatment through B cell TLR4 signal pathway.
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http://dx.doi.org/10.1016/j.nano.2021.102491DOI Listing
November 2021

The Potential Effects of Dielectric Barrier Discharge Plasma on the Extraction Efficiency of Bioactive Compounds in .

Front Nutr 2021 26;8:735742. Epub 2021 Oct 26.

School of Nuclear Science and Technology, University of Science and Technology of China, Hefei, China.

(RPA) is a kind of herbal medicine of traditional Chinese medicine (TCM) that is widely used for the treatment of liver diseases and rheumatoid arthritis in clinical practice. As a result of the low extraction efficiency of RPA by the conventional method, many patients are given high dosages. In this study, four exposure doses of dielectric barrier discharge (DBD) plasma (0, 60, 120, and 180 s) were applied to modify the extraction efficiency of paeoniflorin, benzoylpaeoniflorin, tannic acid, gallic acid, 2'-hydroxy-4'-methoxyacetophenone, and polysaccharide in RPA. Finally, the application of plasma for 180 s exhibited a 24.6% and 12.0% ( < 0.001) increase of tannic acid and polysaccharide contents, however, a 2.1% ( < 0.05) and 5.4% ( < 0.001) reduction of paeoniflorin and gallic acid composition, respectively, and no significant difference ( > 0.05) in results obtained from benzoylpaeoniflorin and 2'-hydroxy-4'-methoxyacetophenone contents. Our results of scanning electron microscopy (SEM), automatic specific surface area and pore analyzer, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and thermal gravimetric analysis (TGA) indicated that DBD plasma can etch the surface and undergo graft polymerization by reactive species thereby changing the water/oil holding capacity and eventually changing the extraction efficiency of bioactive compounds in RPA. Overall, our observations provide a scientific foundation for modifying the extraction efficiency of bioactive ingredients related to the pharmacological activities of RPA.
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http://dx.doi.org/10.3389/fnut.2021.735742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576355PMC
October 2021

Comparison of the Efficacy and Safety of Extracorporeal Shock Wave Lithotripsy and Flexible Ureteroscopy for Treatment of Urolithiasis in Horseshoe Kidney Patients: A Systematic Review and Meta-Analysis.

Front Surg 2021 25;8:726233. Epub 2021 Oct 25.

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.

Urolithiasis is the most common complication of horseshoe kidney (HK), which can be treated by extracorporeal shock wave lithotripsy (ESWL), flexible ureteroscopy (FURS), and percutaneous nephrolithotomy (PCNL). When comparing treatments of ESWL and FURS, it is unclear which is more efficient and safe. The objective of this study was to compare the efficacy and safety of FURS and SWL for the treatment of urolithiasis in HK patients. A systematic search of the Web of Science, PubMed, and EMBASE was performed in February 2021. Newcastle-Ottawa Scale (NOS) was used to assess the risk of bias in each study. Five studies published between 2008 and 2018 were synthesized in the present meta-analysis. The study revealed that FURS compared with SWL had greater initial and overall stone-free rates (SFRs). Risk ratios (RRs) were 2.46 ( < 0.00001) in initial SFRs, 1.36 ( = 0.02) in overall SFRs. No differences were found in the retreatment ratio, RRs were 0.49 ( = 0.43). In addition, no major complications were encountered, and all the complications were mild to moderate. The study demonstrated that FURS and SWL are effective and safe treatments for patients with HK with stones (<20 mm). Moreover, FURS has greater clearance rates and lower complication rates than SWL.
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http://dx.doi.org/10.3389/fsurg.2021.726233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572974PMC
October 2021

Metasurfaces with single-sized antennas for reconstructing full-color holographic images without cross talk.

Opt Lett 2021 Nov;46(21):5417-5420

Designing a color hologram with conventional metasurfaces usually resorts to a supercell strategy or single-sized approach with different incident angles. However, these designs still have their own drawbacks that need to be further solved. Herein, we show a new, to the best of our knowledge, single-sized strategy to design full-color geometric meta-holograms by utilizing the conjugation property of two circularly polarized lights with opposite handedness and diffraction dispersion. The experimentally captured holographic color images are reconstructed with high quality and without cross talk, which agrees well with our theoretical prediction. Moreover, only with an appropriate combination of wavelength and polarization state can color images be observed accurately. Our strategy provides a simple and effective approach for full-color meta-holography and offers significant potential in image display, information storage, etc.
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http://dx.doi.org/10.1364/OL.442477DOI Listing
November 2021

Effects of Tranexamic Acid on Bleeding in Pediatric Surgeries: A Systematic Review and Meta-Analysis.

Front Surg 2021 13;8:759937. Epub 2021 Oct 13.

Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, China.

Major pediatric surgeries can cause severe intraoperative blood loss. This meta-analysis aims to evaluate the efficacy of tranexamic acid (TXA) in pediatric surgeries. We searched PubMed, Embase, Web of Science, and Cochrane Library from the conception to March 31, 2021 to identify eligible randomized controlled trials (RCTs) that evaluated the efficacy of TXA in pediatric surgeries. Two reviewers choosed studies, evaluated quality, extracted data, and assessed the risk of bias independently. Mean difference (MD) was calculated as the summary statistic for continuous data. We used a random-effects model to measure mean effects. Data were generated from the corresponding 95% confidence interval (CI) using RevMan 5.3 software. Primary outcomes included intraoperative and postoperative blood loss, red blood cell (RBC) transfusion as well as fresh frozen plasma (FFP) transfusion. Fifteen studies enrolling 1,332 patients were included in this study. The pooled outcomes demonstrated that TXA was associated with a decreased intraoperative (MD = -1.57 mL/kg, 95% CI, -2.54 to -0.60, = 0.002) and postoperative (MD = -7.85 mL/kg, 95% CI, -10.52 to -5.19, < 0.001) blood loss, a decreased intraoperative (MD = -7.08 mL/kg, 95% CI, -8.01 to -6.16, < 0.001) and postoperative (MD = -5.30 mL/kg, 95% CI, -6.89 to -3.70, < 0.001) RBC transfusion, as well as a decreased intraoperative (MD = -2.74 mL/kg, 95% CI, -4.54 to -0.94, = 0.003) and postoperative (MD = -6.09 mL/kg, 95% CI, -8.26 to -3.91, < 0.001) FFP transfusion in pediatric surgeries. However, no significant difference was noted between two groups in duration of surgery (MD = -12.51 min, 95% CI -36.65 to 11.63, = 0.31). Outcomes of intraoperative and postoperative blood loss and the duration of surgery in included studies were not pooled due to the high heterogeneity. This meta-analysis demonstrated that TXA was beneficial for bleeding in pediatric surgeries.
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http://dx.doi.org/10.3389/fsurg.2021.759937DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548606PMC
October 2021

Efficient, Flexible Autoinduction Expression Systems with Broad Initiation in .

ACS Synth Biol 2021 11 26;10(11):3084-3093. Epub 2021 Oct 26.

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.

Low expression levels and inflexible induction initiation have been the main obstacles to produce proteins using bacterial quorum sensing (QS). The typical QS system in , ComQXPA, activates the promoter P using ComX and ComA as an auto-inducer and a promoter activator, respectively. Here, we developed a series of flexible autoinduction expression systems in WB600 based on ComQXPA using a super-folder green fluorescent protein as the reporter. The -35 region of P was replaced with corresponding conserved sequences of σA-dependent promoters, yielding P with 85% enhanced strength. We then applied a semi-rational design within the spacer between the -35 and -15 regions of P to generate the QS promoter P, which generated 8.22-fold more expression than P. Based on P, we finally obtained three types of autoinduction expression systems with initiation ranging from 1.5-9.5 h by optimizing the combination of the promoters for ComX and ComA. The yield of pullulanase generated using autoinduction expression systems in reached 80.2 U/mL, which was 36% more than that of the most powerful constitutive promoter P. Flexible autoinduction expression systems with diverse dynamic features have considerable potential for improving protein expression and metabolite production in .
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http://dx.doi.org/10.1021/acssynbio.1c00369DOI Listing
November 2021

Comparison of Micro-Percutaneous and Mini-Percutaneous Nephrolithotomy in the Treatment of Renal Stones: A Systematic Review and Meta-Analysis.

Front Surg 2021 4;8:743017. Epub 2021 Oct 4.

Department of Urology, Institute of Urology (Laboratory of Reconstructive Urology), West China Hospital, Sichuan University, Chengdu, China.

To assess the efficacy and safety of micro-percutaneous nephrolithotomy (Microperc) and mini-percutaneous nephrolithotomy (Miniperc) in the treatment of moderately sized renal stones. Literature search of PubMed, Web of Science, and Embase was performed prior to January 2021. We used odds ratios (OR) and weighted mean difference (WMD) for dichotomous variables and continuous variables, respectively. Results were pooled using Review Manager version 5.3 software. A total of six studies involving 291 Microperc and 328 Miniperc cases was included. The overall stone-free rate (SFR) of Microperc was 87.29% (254/291), while the SFR of Miniperc was 86.59% (284/328). Microperc was associated with lower hemoglobin drop (WMD: -0.98; = 0.03) and higher renal colic requiring D-J stent insertion (OR: 3.49; = 0.01). No significant differences existed between Microperc and Miniperc with respect to SFR (OR: 1.10; = 0.69), urinary tract infection (OR: 0.38; = 0.18), operative time (WMD: -5.76; = 0.62), and hospital stay time (WMD: -1.04; = 0.07). Our meta-analysis demonstrated that Microperc could produce an SFR that was comparable with that of Miniperc. Microperc was associated with lower hemoglobin drop, while Miniperc was associated with lower renal colic rates. In addition, the operation time and hospital stay time for both these procedures were similar.
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http://dx.doi.org/10.3389/fsurg.2021.743017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522995PMC
October 2021

Effectiveness and safety of robotic gastrectomy versus laparoscopic gastrectomy for gastric cancer: a meta-analysis of 12,401 gastric cancer patients.

Updates Surg 2021 Oct 16. Epub 2021 Oct 16.

Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, No. 37 Guo Xue Xiang Street, Chengdu, 610041, Sichuan Province, China.

Advanced minimally invasive techniques, such as robotic surgeries, are applied increasingly frequently around the world and are primarily used to improve the surgical outcomes of laparoscopic gastrectomy (LG). Against that background, we conducted a meta-analysis to evaluate the feasibility, safety, and effectiveness of robotic gastrectomy (RG). Studies comparing surgical outcomes between LG and RG patients were retrieved from medical databases, including RCTs and non-RCTs. The primary outcome of this study was overall survival, which was obtained by evaluating the 3-year survival rate and the 5-year survival rate. In addition, postoperative complications, mortality, length of hospital stay, and harvested lymph nodes were also assessed. We also conducted subgroup analyses stratified by resection type, body mass index, age, depth of invasion and tumour size. Ultimately, 31 articles met the criterion for our study through an attentive check of each text, including 1 RCT and 30 non-RCTs. A total of 12,401 patients were included in the analysis, with 8127 (65.5%) undergoing LG and 4274 (34.5%) undergoing RG. Compared with LG, RG was associated with fewer postoperative complications (OR 0.81; 95% CI 0.71-0.93; P = 0.002), especially pancreas-related complications (OR 0.376; 95% CI 0.156-0.911; P = 0.030), increased harvested lymph nodes (WMD 2.03; 95% CI 0.95-3.10; P < 0.001), earlier time to first flatus (WMD - 0.105 days; 95% CI - 0.207 to - 0.003; P = 0.044), longer operation time (WMD 40.192 min, 95% CI 32.07-48.31; P < 0.001), less intraoperative blood loss (WMD - 20.09 ml; 95% CI - 26.86 to - 13.32; P < 0.001), and higher expense (WMD 19,141.68 RMB; 95% CI 11,856.07-26,427.29; P < 0.001). There was no significant difference between RG and LG regarding 3-year overall survival (OR 1.030; 95% CI 0.784-1.353; P = 0.832), 5-year overall survival (OR 0.862; 95% CI 0.721-1.031; P = 0.105), conversion rate (OR 0.857; 95% CI 0.443-1.661; P = 0.648), postoperative hospital stay (WMD - 0.368 days; 95% CI - 0.75-0.013; P = 0.059), mortality (OR 1.248; 95% CI 0.514-3.209; P = 0.592), and reoperation (OR 0.855; 95% CI 0.479-1.525; P = 0.595). Our study revealed that postoperative complications, especially pancreas-related complications, occurred less often with RG than with LG. However, long-term outcomes between the two surgical techniques need to be further examined, particularly regarding the oncological adequacy of robotic gastric cancer resections.
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http://dx.doi.org/10.1007/s13304-021-01176-3DOI Listing
October 2021

Neuromedin B receptor stimulation of Cav3.2 T-type Ca channels in primary sensory neurons mediates peripheral pain hypersensitivity.

Theranostics 2021 9;11(19):9342-9357. Epub 2021 Sep 9.

Department of Physiology and Neurobiology & Centre for Ion Channelopathy, Medical College of Soochow University, Suzhou 215123, China.

Neuromedin B (Nmb) is implicated in the regulation of nociception of sensory neurons. However, the underlying cellular and molecular mechanisms remain unknown. Using patch clamp recording, western blot analysis, immunofluorescent labelling, enzyme-linked immunosorbent assays, adenovirus-mediated shRNA knockdown and animal behaviour tests, we studied the effects of Nmb on the sensory neuronal excitability and peripheral pain sensitivity mediated by Cav3.2 T-type channels. Nmb reversibly and concentration-dependently increased T-type channel currents ( ) in small-sized trigeminal ganglion (TG) neurons through the activation of neuromedin B receptor (NmbR). This NmbR-mediated response was G protein-coupled, but independent of protein kinase C activity. Either intracellular application of the QEHA peptide or shRNA-mediated knockdown of G abolished the NmbR-induced response. Inhibition of protein kinase A (PKA) or AMP-activated protein kinase (AMPK) completely abolished the Nmb-induced response. Analysis of phospho-AMPK (-AMPK) revealed that Nmb significantly activated AMPK, while AMPK inhibition prevented the Nmb-induced increase in PKA activity. In a heterologous expression system, activation of NmbR significantly enhanced the Cav3.2 channel currents, while the Cav3.1 and Cav3.3 channel currents remained unaffected. Nmb induced TG neuronal hyperexcitability and concomitantly induced mechanical and thermal hypersensitivity, both of which were attenuated by T-type channel blockade. Moreover, blockade of NmbR signalling prevented mechanical hypersensitivity in a mouse model of complete Freund's adjuvant-induced inflammatory pain, and this effect was attenuated by siRNA knockdown of Cav3.2. Our study reveals a novel mechanism by which NmbR stimulates Cav3.2 channels through a G-dependent AMPK/PKA pathway. In mouse models, this mechanism appears to drive the hyperexcitability of TG neurons and induce pain hypersensitivity.
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http://dx.doi.org/10.7150/thno.62255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490515PMC
September 2021

Exogenous Nicotinamide Adenine Dinucleotide Attenuates Postresuscitation Myocardial and Neurologic Dysfunction in a Rat Model of Cardiac Arrest.

Crit Care Med 2021 Sep 22. Epub 2021 Sep 22.

Department of Emergency Medicine Center, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China. Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA. Department of Critical Care Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Department of Emergency Medicine, The Second Affiliated Hospital of Soochow University, Soochow, China. Departments of Internal Medicine and Emergency Medicine, Virginia Commonwealth University Health System, Richmond, VA. Department of Emergency Medicine, Virginia Commonwealth University Health System, Richmond, VA. Department of Surgery, Virginia Commonwealth University Health System, Richmond, VA.

Objectives: To investigate the therapeutic potential and underlying mechanisms of exogenous nicotinamide adenine dinucleotide+ on postresuscitation myocardial and neurologic dysfunction in a rat model of cardiac arrest.

Design: Thirty-eight rats were randomized into three groups: 1) Sham, 2) Control, and 3) NAD. Except for the sham group, untreated ventricular fibrillation for 6 minutes followed by cardiopulmonary resuscitation was performed in the control and NAD groups. Nicotinamide adenine dinucleotide+ (20 mg/kg) was IV administered at the onset of return of spontaneous circulation.

Setting: University-affiliated research laboratory.

Subjects: Sprague-Dawley rats.

Interventions: Nicotinamide adenine dinucleotide+.

Measurements And Main Results: Hemodynamic and myocardial function were measured at baseline and within 4 hours following return of spontaneous circulation. Survival analysis and Neurologic Deficit Score were performed up to 72 hours after return of spontaneous circulation. Adenosine triphosphate (adenosine triphosphate) level was measured in both brain and heart tissue. Mitochondrial respiratory chain function, acetylation level, and expression of Sirtuin3 and NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9 (NDUFA9) in isolated mitochondrial protein from both brain and heart tissue were evaluated at 4 hours following return of spontaneous circulation. The results demonstrated that nicotinamide adenine dinucleotide+ treatment improved mean arterial pressure (at 1 hr following return of spontaneous circulation, 94.69 ± 4.25 mm Hg vs 89.57 ± 7.71 mm Hg; p < 0.05), ejection fraction (at 1 hr following return of spontaneous circulation, 62.67% ± 6.71% vs 52.96% ± 9.37%; p < 0.05), Neurologic Deficit Score (at 24 hr following return of spontaneous circulation, 449.50 ± 82.58 vs 339.50 ± 90.66; p < 0.05), and survival rate compared with that of the control group. The adenosine triphosphate level and complex I respiratory were significantly restored in the NAD group compared with those of the control group. In addition, nicotinamide adenine dinucleotide+ treatment activated the Sirtuin3 pathway, down-regulating acetylated-NDUFA9 in the isolated mitochondria protein.

Conclusions: Exogenous nicotinamide adenine dinucleotide+ treatment attenuated postresuscitation myocardial and neurologic dysfunction. The responsible mechanisms may involve the preservation of mitochondrial complex I respiratory capacity and adenosine triphosphate production, which involves the Sirtuin3-NDUFA9 deacetylation.
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http://dx.doi.org/10.1097/CCM.0000000000005268DOI Listing
September 2021

Effect of non-ionizing reaction rate (assumed to be controllable) on the plasma generation mechanism and communication around RAMC vehicle during atmospheric reentry.

Sci Rep 2021 Oct 8;11(1):20046. Epub 2021 Oct 8.

School of Nuclear Science and Technology, University of Science and Technology of China, Hefei, 230026, People's Republic of China.

Radio frequency (RF) blackout occurs during radio attenuation measurement C (RAMC) vehicle reentry due to the attenuation effect of the plasma sheath on the communication signal. In recent years, the mitigation mechanism of chemical reaction for RF blackout problem has gradually been studied numerically and experimentally. However, the effect of non-ionization reaction rate has been ignored because it does not directly involve the generation of electrons. In the present study, the influence of non-ionizing reaction rate on the plasma generation mechanism and EM wave attenuation was numerically solved by the plasma flow and multilayer transmission model. According to the simulation results, only the reaction rate of [Formula: see text] has a significant effect on the electron number density in all non-ionizing reactions, and the degree of influence is less than the ionization reaction rate. The EM wave attenuation decreases with the decrease of the reaction rate of [Formula: see text]. When the reaction rate is reduced by 25 times, the maximum attenuation of electromagnetic wave can be reduced by 12 dB. Finally, a potential scheme by reducing the reaction rate of [Formula: see text] was proposed to mitigate the RF blackout problem.
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http://dx.doi.org/10.1038/s41598-021-99584-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501113PMC
October 2021

UAMC-3203 OR/and Deferoxamine Improve Post-Resuscitation Myocardial Dysfunction Through Suppressing Ferroptosis In A Rat Model of Cardiac Arrest.

Shock 2021 Oct 5. Epub 2021 Oct 5.

School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan Province, China School of Medicine, Anhui University of Science & Technology, Huainan, Anhui Province, China Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, Virginia Third People's Hospital of Chengdu Affiliated to Southwest Jiaotong University, Chengdu, Sichuan Province, China Departments of Internal Medicine and Emergency Medicine, Virginia Commonwealth University Health System, Richmond, Virginia Department of Emergency Medicine, Virginia Commonwealth University Health System, Richmond, Virginia.

Abstract: Blocking ferroptosis reduces ischemia-reperfusion injury in some pathological contexts. However, there is no evidence that ferroptosis contributes to post-resuscitation myocardial dysfunction (PRMD). Here, we evaluated the therapeutic performance of ferroptosis inhibitors (UAMC-3203 or/and Deferoxamine) on the PRMD in a rat model of cardiac arrest and surveyed the changes of essential ferroptosis markers in the myocardium. Remarkably, all treatments reduce the severity of cardiac dysfunction and microcirculation hypoperfusion after resuscitation compared with control. Consistently, we observe that the ferroptosis marker Glutathione peroxidase 4, 4-hydroxynonenal and non-heme iron altered (1 ± 0.060 vs. 0.021 ± 0.016, 1 ± 0.145 vs. 3.338 ± 0.221, 52.010 ± 3.587 ug/g vs. 70.500 ± 3.158 ug/g, all P < 0.05) in the myocardium after resuscitation. These changes were significantly suppressed by UAMC-3203 [(0.187 ± 0.043, 2.848 ± 0.169, all P < 0.05), (72.43 ± 4.920 ug/g, P  > 0.05)], or Deferoxamine (0.203 ± 0.025, 2.683 ± 0.273, 55.95 ± 2.497 ug/g, all P < 0.05). Briefly, UAMC-3203 or/and Deferoxamine improve post-resuscitation myocardial dysfunction and provide evidence of ferroptosis involvement, suggesting that ferroptosis inhibitors could potentially provide an innovative therapeutic approach for mitigating the myocardial damage caused by cardiopulmonary resuscitation.
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http://dx.doi.org/10.1097/SHK.0000000000001869DOI Listing
October 2021

Photonic time-stretch based on phase modulation for sub-octave applications.

Appl Opt 2021 Aug;60(22):6487-6494

Photonic time-stretch (PTS) has been extensively studied due to its great potential in analog-to-digital converters. Here, we propose and demonstrate a PTS system based on phase modulation for sub-octave applications. Different from the PTS system using a Mach-Zehnder modulator (MZM), the PTS system, which uses a phase modulator (PM), has an operation bandwidth within an octave and is more suitable for preprocessing of sub-octave signals. Within the sub-octave band, the system is free of all second-order spurious signals. Because there is no direct current bias in a PM, the problem of bias drift, as well as the nonlinear distortion caused by it, can be thoroughly avoided. In addition, based on phase modulation and direct detection, the proposed PTS system has higher stability and a more simplified structure than that based on coherent detection. An exact analytical model has been established, and some compact expressions have been derived to fully characterize all frequency components of the PM-based PTS system. System properties, including the power transfer function, 3-dB bandwidth, and nonlinear distortion have been discussed, and numerical and experimental results on the performance of the PM-based PTS have been presented. In addition, a dual-channel PTS that employs a PM and a push-pull MZM has been proposed to extend the operation bandwidth to multi-octave.
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http://dx.doi.org/10.1364/AO.430215DOI Listing
August 2021

Developing Strategy to Predict the Results of Prostate Multiparametric Magnetic Resonance Imaging and Reduce Unnecessary Multiparametric Magnetic Resonance Imaging Scan.

Front Oncol 2021 14;11:732027. Epub 2021 Sep 14.

Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Purpose: The clinical utility of multiparametric magnetic resonance imaging (mpMRI) for the detection and localization of prostate cancer (PCa) has been evaluated and validated. However, the implementation of mpMRI into the clinical practice remains some burden of cost and availability for patients and society. We aimed to predict the results of prostate mpMRI using the clinical parameters and multivariable model to reduce unnecessary mpMRI scans.

Methods: We retrospectively identified 784 men who underwent mpMRI scans and subsequent prostate biopsy between 2016 and 2020 according to the inclusion criterion. The cohort was split into a training cohort of 548 (70%) patients and a validation cohort of 236 (30%) patients. Clinical parameters including age, prostate-specific antigen (PSA) derivates, and prostate volume (PV) were assessed as the predictors of mpMRI results. The mpMRI results were divided into groups according to the reports: "negative", "equivocal", and "suspicious" for the presence of PCa.

Results: Univariate analysis showed that the total PSA (tPSA), free PSA (fPSA), PV, and PSA density (PSAD) were significant predictors for suspicious mpMRI ( < 0.05). The PSAD (AUC = 0.77) and tPSA (AUC = 0.74) outperformed fPSA (AUC = 0.68) and PV (AUC = 0.62) in the prediction of the mpMRI results. The multivariate model (AUC = 0.80) had a similar diagnostic accuracy with PSAD ( = 0.108), while higher than tPSA ( = 0.024) in predicting the mpMRI results. The multivariate model illustrated a better calibration and substantial improvement in the decision curve analysis (DCA) at a threshold above 20%. Using the PSAD with a 0.13 ng/ml cut-off could spare the number of mpMRI scans by 20%, keeping a 90% sensitivity in the prediction of suspicious MRI-PCa and missing three (3/73, 4%) clinically significant PCa cases. At the same sensitivity level, the multivariate model with a 32% cut-off could spare the number of mpMRI scans by 27%, missing only one (1/73, 1%) clinically significant PCa case.

Conclusion: Our multivariate model could reduce the number of unnecessary mpMRI scans without comprising the diagnostic ability of clinically significant PCa. Further prospective validation is required.
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http://dx.doi.org/10.3389/fonc.2021.732027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476778PMC
September 2021

Kaistella beijingensis sp. nov., Isolated from a Municipal Wastewater Treatment Plant, Is Involved in Sludge Foaming.

Appl Environ Microbiol 2021 11 29;87(24):e0153421. Epub 2021 Sep 29.

State Key Laboratory of Microbial Resources and Environmental Microbiology Research Center at Institute of Microbiology, Chinese Academy of Sciencesgrid.9227.e, Beijing, China.

Biological foaming (or biofoaming) is a frequently occurring problem in wastewater treatment plants (WWTPs) and is attributed to the overwhelming growth of filamentous bulking and foaming bacteria (BFB). Biological foaming has been intensively investigated, with BFB like and having been identified from WWTPs and implicated in foaming. Nevertheless, studies are still needed to improve our understanding of the microbial diversity of WWTP biofoams and how microbial activities contribute to foaming. In this study, sludge foaming at the Qinghe WWTP of China was monitored, and sludge foams were investigated using culture-dependent and culture-independent microbiological methods. The foam microbiomes exhibited high abundances of , Mycobacterium, , and . A previously unknown bacterium, Kaistella beijingensis, was cultivated from foams, its genome was sequenced, and it was phenotypically characterized. . K. beijingensis exhibits hydrophobic cell surfaces, produces extracellular polymeric substances (EPS), and metabolizes lipids. . K. beijingensis abundances were proportional to EPS levels in foams. Several proteins encoded by the . K. beijingensis genome were identified from EPS that was extracted from sludge foams. . K. beijingensis populations accounted for 4 to 6% of the total bacterial populations in sludge foam samples within the Qinghe WWTP, although their abundances were higher in spring than in other seasons. Cooccurrence analysis indicated that . K. beijingensis was not a core node among the WWTP community network, but its abundances were negatively correlated with those of the well-studied BFB Skermania piniformis among cross-season Qinghe WWTP communities. Biological foaming, also known as scumming, is a sludge separation problem that has become the subject of major concern for long-term stable activated sludge operation in decades. Biological foaming was considered induced by foaming bacteria. However, the occurrence and deterioration of foaming in many WWTPs are still not completely understood. Cultivation and characterization of the enriched bacteria in foaming are critical to understand their genetic, physiological, phylogenetic, and ecological traits, as well as to improve the understanding of their relationships with foaming and performance of WWTPs.
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http://dx.doi.org/10.1128/AEM.01534-21DOI Listing
November 2021

Re: Guohua Zeng, Chao Cai, Xianzhong Duan, et al. Mini Percutaneous Nephrolithotomy Is a Noninferior Modality to Standard Percutaneous Nephrolithotomy for the Management of 20-40 mm Renal Calculi: A Multicenter Randomized Controlled Trial. Eur Urol 2021;80:114-21.

Eur Urol 2021 Dec 16;80(6):e150. Epub 2021 Sep 16.

Department of Urology, Institute of Urology, Laboratory of Reconstructive Urology, West China Hospital, Sichuan University, Chengdu, China. Electronic address:

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http://dx.doi.org/10.1016/j.eururo.2021.09.003DOI Listing
December 2021

MRI magnetic compatible electrical neural interface: From materials to application.

Biosens Bioelectron 2021 Dec 1;194:113592. Epub 2021 Sep 1.

Laboratory for Neural Interface and Brain Computer Interface, Institute of AI and Robotics, Academy for Engineering and Technology, FUDAN University, 220 Handan Rd., Yangpu District, Shanghai, 200433, China; Ji Hua Laboratory, 28 Island Ring South Rd., Foshan City, 528200, China; Engineering Research Center of AI & Robotics, Ministry of Education, Shanghai Engineering Research Center of AI & Robotics, MOE Frontiers Center for Brain Science, Shanghai 200433, China; Research Center for Intelligent Sensing, Zhejiang Lab, Hangzhou, 311100, China; Yiwu Research Institute of Fudan University, Chengbei Road, Yiwu City, 322000, Zhejiang, China. Electronic address:

Neural electrical interfaces are important tools for local neural stimulation and recording, which potentially have wide application in the diagnosis and treatment of neural diseases, as well as in the transmission of neural activity for brain-computer interface (BCI) systems. At the same time, magnetic resonance imaging (MRI) is one of the effective and non-invasive techniques for recording whole-brain signals, providing details of brain structures and also activation pattern maps. Simultaneous recording of extracellular neural signals and MRI combines two expressions of the same neural activity and is believed to be of great importance for the understanding of brain function. However, this combination makes requests on the magnetic and electronic performance of neural interface devices. MRI-compatibility refers here to a technological approach to simultaneous MRI and electrode recording or stimulation without artifacts in imaging. Trade-offs between materials magnetic susceptibility selection and electrical function should be considered. Herein, prominent trends in selecting materials of suitable magnetic properties are analyzed and material design, function and application of neural interfaces are outlined together with the remaining challenge to fabricate MRI-compatible neural interface.
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http://dx.doi.org/10.1016/j.bios.2021.113592DOI Listing
December 2021

13C-Urea Breath Test for the Diagnosis of Infection in Patients after Partial Gastrectomy: A Systematic Review and Meta-Analysis.

J Invest Surg 2021 Sep 7:1-10. Epub 2021 Sep 7.

Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Background: The C-urea breath test (C-UBT) is a kind of safe, noninvasive, and reliable measure for () infection diagnosis in patients with complete stomach. Nevertheless, the test has not indicated fully precision in people who have had a gastrectomy. So, we made the use of a systematic review of plentiful published resources and research and meta-analysis. There are prominent research achievements regarding of utilizing C-UBT for infection patients diagnosis with the residual stomach.

Method: We searched publications available on Cochrane, PubMed, and Embase databases, and on the web of science. The last search was performed in May 2021. The basis model for this meta-analysis was fixed-effect through Metadisc Beta 1.4 software (Universidad Complutense, Madrid, Spain). When measuring the precision of C-urea breath test, we utilized the ratio analysis such as Ratio of diagnostic odds ratio (DOR) and its corresponding 95% confidence interval (95%CI).

Results: Ten associated researches were analyzed with a total of 1065 patients. In general, the sensitivity of included studies ranged from 0.40 to 1.00 (I = 81.2%), whereas the specificity ranged from 0.59 to 1.00 (I = 90.1%). The pooled sensitivity, specificity were 0.83 (95% CI, 0.79-0.86;  < 0.001), 0.79 (95% CI, 0.76-0.83;  < 0.001) respectively. The accuracy ranged from 71 to 99. The positive predictive values ranged from 45 to 100. The negative predictive values ranged from 53 to 100. The combined DOR was 36.02 (95%CI 15.65-82.92; I = 65.8%;  = 0.0018). The corresponding AUC for the SROC curve was 0.92 and the Q value was 0.85.

Conclusion: In conclusion, the outcomes of meta-analysis indicate that during the period of patients' diagnosis in infection, who undergoes partial gastrectomy, has high accuracy.
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http://dx.doi.org/10.1080/08941939.2021.1972185DOI Listing
September 2021

Role of the PD-1/PD-L1 Signaling in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis: Recent Insights and Future Directions.

Mol Neurobiol 2021 Sep 3. Epub 2021 Sep 3.

Department of Neurology and Neuroscience Center, The First Hospital of Jilin University, Xinmin Street 71#, Changchun, 130021, China.

Multiple sclerosis (MS) is an autoimmunity-related chronic demyelination disease of the central nervous system (CNS), causing young disability. Currently, highly specific immunotherapies for MS are still lacking. Programmed cell death 1 (PD-1) is an immunosuppressive co-stimulatory molecule, which is expressed on activated T lymphocytes, B lymphocytes, natural killer cells, and other immune cells. PD-L1, the ligand of PD-1, is expressed on T lymphocytes, B lymphocytes, dendritic cells, and macrophages. PD-1/PD-L1 delivers negative regulatory signals to immune cells, maintaining immune tolerance and inhibiting autoimmunity. This review comprehensively summarizes current insights into the role of PD-1/PD-L1 signaling in MS and its animal model experimental autoimmune encephalomyelitis (EAE). The potentiality of PD-1/PD-L1 as biomarkers or therapeutic targets for MS will also be discussed.
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http://dx.doi.org/10.1007/s12035-021-02495-7DOI Listing
September 2021

High-resolution respirometry for evaluation of mitochondrial function on brain and heart homogenates in a rat model of cardiac arrest and cardiopulmonary resuscitation.

Biomed Pharmacother 2021 Oct 30;142:111935. Epub 2021 Aug 30.

Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA; Department of Emergency Medicine, Virginia Commonwealth University Health System, Richmond, VA, USA. Electronic address:

The physiology and physiopathology process of mitochondrial function following cardiac arrest remains poorly understood. We aimed to assess mitochondrial respiratory function on the heart and brain homogenates from cardiac arrest rats. The expression level of SIRT1/PGC-1α pathway was measured by immunoblotting. 30 rats were assigned to the CA group and the sham group. Rats of CA were subjected to 6 min of untreated ventricular fibrillation (VF) followed by 8 min of cardiopulmonary resuscitation (CPR). Mitochondrial respiratory function was compromised following CA and I/R injury, as indicated by CI (451.46 ± 71.48 vs. 909.91 ± 5.51 pmol/min*mg for the heart and 464.14 ± 8.22 vs. 570.53 ± 56.33 pmol/min*mg for the brain), CI (564.04 ± 64.34 vs. 2729.52 ± 347.39 pmol/min*mg for the heart and 726.07 ± 85.78 vs. 1762.82 ± 262.04 pmol/min*mg for the brain), RCR (1.88 ± 0.46 vs. 3.57 ± 0.38 for the heart and 2.05 ± 0.19 vs. 3.49 ± 0.19, for the brain) and OXPHOS coupling efficiency (0.45 ± 0.11 vs. 0.72 ± 0.03 for the heart and 0.52 ± 0.05 vs. 0.71 ± 0.01 for the brain). However, routine respiration was lower in the heart and comparable in the brain after CA. CIV did not change in the heart but was enhanced in the brain. Furthermore, both SIRT1 and PGC-1α were downregulated concurrently in the heart and brain. The mitochondrial respiratory function was compromised following CA and I/R injury, and the major affected respiratory state is complex I-linked respiration. Furthermore, the heart and the brain respond differently to the global I/R injury after CA in mitochondrial respiratory function. Inhibition of the SIRT1/PGC-1α pathway may be a major contributor to the impaired mitochondrial respiratory function.
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http://dx.doi.org/10.1016/j.biopha.2021.111935DOI Listing
October 2021

Roles of the Immune/Methylation/Autophagy Landscape on Single-Cell Genotypes and Stroke Risk in Breast Cancer Microenvironment.

Oxid Med Cell Longev 2021 19;2021:5633514. Epub 2021 Aug 19.

Department of General Surgery, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, 200080, China.

This study sought to perform integrative analysis of the immune/methylation/autophagy landscape on breast cancer prognosis and single-cell genotypes. Breast Cancer Recurrence Risk Score (BCRRS) and Breast Cancer Prognostic Risk Score (BCPRS) were determined based on 6 prognostic IMAAGs obtained from the TCGA-BRCA cohort. BCRRS and BCPRS, respectively, were used to construct a risk prediction model of overall survival and progression-free survival. Predictive capacity of the model was evaluated using clinical data. Analysis showed that BCRRS is associated with a high risk of stroke. In addition, PPI and drug-ceRNA networks based on differences in BCPRS were constructed. Single cells were genotyped through integrated scRNA-seq of the TNBC samples based on clustering results of BCPRS-related genes. The findings of this study show the potential regulatory effects of IMAAGs on breast cancer tumor microenvironment. High AUCs of 0.856 and 0.842 were obtained for the OS and PFS prognostic models, respectively. scRNA-seq analysis showed high expression levels of adipocytes and adipose tissue macrophages (ATMs) in high BCPRS clusters. Moreover, analysis of ligand-receptor interactions and potential regulatory mechanisms were performed. The LINC00276&MALAT1/miR-206/FZD4-Wnt7b pathway was also identified which may be useful in future research on targets against breast cancer metastasis and recurrence. Neural network-based deep learning models using BCPRS-related genes showed that these genes can be used to map the tumor microenvironment. In summary, analysis of IMAAGs, BCPRS, and BCRRS provides information on the breast cancer microenvironment at both the macro- and microlevels and provides a basis for development of personalized treatment therapy.
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http://dx.doi.org/10.1155/2021/5633514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397558PMC
September 2021

Cold atmospheric plasma: A non-negligible strategy for viral RNA inactivation to prevent SARS-CoV-2 environmental transmission.

AIP Adv 2021 Aug 10;11(8):085019. Epub 2021 Aug 10.

Anhui Academy of Medical Sciences, Hefei, China.

Cold atmospheric plasma (CAP), regarded as a powerful physics technology, displays antimicrobial, antitumor, and even antiviral properties, but the underlying mechanism is rarely studied. In this study, four CAP exposure doses (30, 60, 120, and 240 s) were applied to inactivate a severe acute respiratory syndrome coronavirus 2 like pseudovirus on a stainless steel disk, which comprised spike protein on its membrane and can express a green fluorescent protein. In order to unravel the potential effects of CAP irradiation on pseudovirus, infection assay, optical emission spectra analysis, transmission electron microscopy (TEM), sodium dodecyl sulfate polyacrylamide gel electrophoresis, ELISA, and qPCR experiments were carried out. As a result, our study indicated that CAP irradiation can significantly decrease the infectivity of pseudovirus in a dose dependent manner through destroying the cell membrane and further damaging viral RNA, with the molecular weight and conformation of spike receptor binding domain protein unchanged.
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http://dx.doi.org/10.1063/5.0060530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371919PMC
August 2021

Electrolyte/Structure-Dependent Cocktail Mediation Enabling High-Rate/Low-Plateau Metal Sulfide Anodes for Sodium Storage.

Nanomicro Lett 2021 Aug 17;13(1):178. Epub 2021 Aug 17.

Department of Materials Science and Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Hong Kong, P. R. China.

As promising anodes for sodium-ion batteries, metal sulfides ubiquitously suffer from low-rate and high-plateau issues, greatly hindering their application in full-cells. Herein, exemplifying carbon nanotubes (CNTs)-stringed metal sulfides superstructure (CSC) assembled by nano-dispersed SnS and CoS phases, cocktail mediation effect similar to that of high-entropy materials is initially studied in ether-based electrolyte to solve the challenges. The high nano-dispersity of metal sulfides in CSC anode underlies the cocktail-like mediation effect, enabling the circumvention of intrinsic drawbacks of different metal sulfides. By utilizing ether-based electrolyte, the reversibility of metal sulfides is greatly improved, sustaining a long-life effectivity of cocktail-like mediation. As such, CSC effectively overcomes low-rate flaw of SnS and high-plateau demerit of CoS, simultaneously realizes a high rate and a low plateau. In half-cells, CSC delivers an ultrahigh-rate capability of 327.6 mAh g at 20 A g, far outperforming those of monometallic sulfides (SnS, CoS) and their mixtures. Compared with CoS phase and SnS/CoS mixture, CSC shows remarkably lowered average charge voltage up to ca. 0.62 V. As-assembled CSC//NaVPOF full-cell shows a good rate capability (0.05 ~ 1.0 A g, 120.3 mAh g at 0.05 A g) and a high average discharge voltage up to 2.57 V, comparable to full-cells with alloy-type anodes. Kinetics analysis verifies that the cocktail-like mediation effect largely boosts the charge transfer and ionic diffusion in CSC, compared with single phase and mixed phases. Further mechanism study reveals that alternative and complementary electrochemical processes between nano-dispersed SnS and CoS phases are responsible for the lowered charge voltage of CSC. This electrolyte/structure-dependent cocktail-like mediation effect effectively enhances the practicability of metal sulfide anodes, which will boost the development of high-rate/-voltage sodium-ion full batteries.
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http://dx.doi.org/10.1007/s40820-021-00686-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371071PMC
August 2021
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