Publications by authors named "Tao Huang"

1,653 Publications

  • Page 1 of 1

Circ_0068087 Silencing Ameliorates Oxidized Low-Density Lipoprotein-Induced Dysfunction in Vascular Endothelial Cells Depending on miR-186-5p-Mediated Regulation of Roundabout Guidance Receptor 1.

Front Cardiovasc Med 2021 26;8:650374. Epub 2021 May 26.

Department of Cardiovascular Medicine, Affiliated Hospital of Weifang Medical University, Weifang, China.

Circular RNAs (circRNAs) are endogenous non-coding RNAs involved in the progression of atherosclerosis (AS). We investigated the role of circ_0068087 in AS progression and its associated mechanism. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay, flow cytometry, and enzyme-linked immunosorbent assay (ELISA) were performed to analyze the viability, apoptosis, and inflammatory response of HUVECs, respectively. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and the Western blot assay were performed to measure the expression of RNA and protein. Cell oxidative stress was analyzed using commercial kits. The dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were conducted to verify the interaction between microRNA-186-5p (miR-186-5p) and circ_0068087 or roundabout guidance receptor 1 (ROBO1). Oxidized low-density lipoprotein (ox-LDL) exposure upregulated the circ_0068087 level in HUVECs. ox-LDL-induced dysfunction in HUVECs was largely attenuated by the silence of circ_0068087. Circ_0068087 negatively regulated the miR-186-5p level by interacting with it in HUVECs. Circ_0068087 knockdown restrained ox-LDL-induced injury in HUVECs partly by upregulating miR-186-5p. ROBO1 was a downstream target of miR-186-5p in HUVECs. Circ_0068087 positively regulated ROBO1 expression by sponging miR-186-5p in HUVECs. MiR-186-5p overexpression exerted a protective role in ox-LDL-induced HUVECs partly by downregulating ROBO1. Circ_0068087 interference alleviated ox-LDL-induced dysfunction in HUVECs partly by reducing ROBO1 expression via upregulating miR-186-5p.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2021.650374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187595PMC
May 2021

Essential Role of CFAP53 in Sperm Flagellum Biogenesis.

Front Cell Dev Biol 2021 28;9:676910. Epub 2021 May 28.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China.

The sperm flagellum is essential for male fertility. Despite vigorous research progress toward understanding the pathogenesis of flagellum-related diseases, much remains unknown about the mechanisms underlying the flagellum biogenesis itself. Here, we show that the cilia and flagella associated protein 53 () gene is predominantly expressed in testes, and it is essential for sperm flagellum biogenesis. The knockout of this gene resulted in complete infertility in male mice but not in the females. CFAP53 localized to the manchette and sperm tail during spermiogenesis, the knockout of this gene impaired flagellum biogenesis. Furthermore, we identified two manchette and sperm tail-associated proteins that interacted with CFAP53 during spermiogenesis. Together, our results suggest that CFAP53 is an essential protein for sperm flagellum biogenesis, and its mutations might be associated with multiple morphological abnormalities of the flagella (MMAF).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.676910DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195676PMC
May 2021

Vaccination control of an epidemic model with time delay and its application to COVID-19.

Nonlinear Dyn 2021 May 28:1-14. Epub 2021 May 28.

School of Science, Chongqing University of Posts and Telecommunications, Chongqing, 400065 China.

This paper studies an SEIR-type epidemic model with time delay and vaccination control. The vaccination control is applied when the basic reproduction number . The vaccination strategy is expressed as a state delayed feedback which is related to the current and previous state of the epidemic model, and makes the model become a linear system in new coordinates. For the presence and absence of vaccination control, we investigate the nonnegativity and boundedness of the model, respectively. We obtain some sufficient conditions for the eigenvalues of the linear system such that the nonnegativity of the epidemic model can be guaranteed when the vaccination strategy is applied. In addition, we study the stability of disease-free equilibrium when and the persistent of disease when . Finally, we use the obtained theoretical results to simulate the vaccination strategy to control the spread of COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11071-021-06533-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162653PMC
May 2021

Check dam storage capacity calculation based on high-resolution topogrammetry: Case study of the Cutou Gully, Wenchuan County, China.

Sci Total Environ 2021 May 28;790:148083. Epub 2021 May 28.

Environmental Mountain Office, Ministry of Science, Technology and Environment of Cuba, Guantánamo, Cuba.

Debris flows are a common natural trigger of disasters in mountainous areas, and check dams are standard structural measures for controlling debris flows. Despite their prevalence in debris flow-prone areas worldwide, the capacity of check dams is still calculated using empirical formulas, which lead to large calculation errors. This paper proposes a new method that uses GIS to calculating the design storage capacity of a check dam in the debris flow-prone Cutou Gully in Wenchuan County, China. Large-scale digital surface models derived from unmanned aerial vehicle imagery and ground surveys identify local topographic changes in the debris flow path and develop appropriate maintenance plans for check dams. The measured storage capacity of the check dam is determined by analyzing the DEM differences. This study uses the newly proposed method to calculate the design storage capacity of the check dam. The accuracy of the calculation results was evaluated using the checkpoint method, and the results showed that the design and measured siltation surface errors ranged from -1.16-2.96 m, with a root mean square error of 0.93 m. The design capacity of the check dam is 33.6× 10 m, and the actual capacity is 36.7× 10 m, with an absolute error of 3.1× 10 m and relative error of 8.6%. The results prove the validity of the proposed calculation method; moreover, this study shows that the new method is accurate, easy to operate, and highly efficient for visualizing the spatial distribution of the siltation depth behind the check dam. This work will help improve future engineering decisions, design strategies, and find optimal design solutions to minimize the risk of debris flow hazards.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.148083DOI Listing
May 2021

Diagnosis of malignant versus tuberculous ascites using tumor markers and globulin ratios in serum and ascites: A Fisher discriminant model.

Arab J Gastroenterol 2021 Jun 2. Epub 2021 Jun 2.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China. Electronic address:

Background And Study Aims: This study was conducted to investigate the significance of tumor and biochemical markers in serum and ascitic fluid in the differential diagnosis of tuberculous and malignant ascites.

Patients And Methods: Based on findings from natural orifice transluminal endoscopic surgery and postoperative pathology or cytology of 63 patients, they were divided into the malignant group (31 patients) and the tuberculous group (32 patients). Levels of tumor markers, albumin, globulin, and lactate dehydrogenase were measured simultaneously. Data were statistically analyzed, and a Fisher discriminant model was established. The receiver operating characteristic curve was constructed to confirm the discriminant value.

Results: The levels of carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), cancer antigen 19-9 (CA 19-9), and globulin in serum and ascitic fluid were different between the tuberculous and malignant ascites groups (P < .05). The ratios of ascites-to-serum levels of CEA, CA125, and CA 19-9, as well as the ratio of serum-to-ascites of globulin levels, were different between the two groups (P < .05). The Fisher discriminant model was established based on the ascites-to-serum ratios of CEA, CA125, and CA 19-9 levels and the serum-to-ascites ratio of globulin levels. The area under the curve was 0.908, the sensitivity was 0.838 (26/31), and the specificity was 0.875 (28/32).

Conclusion: A Fisher discriminant model can be established using serum and ascites tumor markers and globulin ratios, which is valuable in the differential diagnosis of tuberculous versus malignant ascites.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ajg.2021.05.002DOI Listing
June 2021

Series of AzTO-Based Energetic Materials: Effect of Different π-π Stacking Modes on Their Thermal Stability and Sensitivity.

Langmuir 2021 Jun 3;37(23):7118-7126. Epub 2021 Jun 3.

State Key Laboratory of Environment-friendly Energy Materials, School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.

π-Stacking is common in materials, but different π-π stacking modes remarkably affect the properties and performances of materials. In particular, weak interactions, π-stacking and hydrogen bonding, often have a great impact on the stability and sensitivity of high-energetic compounds. Therefore, several of energetic materials based on 1,1'-dihydroxyazotetrazole () with a nearly flat structure, such as the salts of aminoguanidine (), 1,3-diaminoguanidine (), imidazole (), pyrazole () and triaminoguanidine (), and a cocrystal of 2-methylimidazole (), were designed and synthesized. Based on single-crystal diffraction data, thermal decomposition behaviors, and the mechanical sensitivity test, the compounds of , , and with face-to-face π-π stacking display outstanding thermal stability and insensitivity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.1c00705DOI Listing
June 2021

Effects of different concentrations of metformin on osteoclast differentiation and apoptosis and its mechanism.

Pharmazie 2021 Jun;76(6):244-248

Department of Spine Surgery and Musculoskeletal Tumor, Zhongnan Hospital of Wuhan University, People's Republic of China;, Email:

This study aimed to investigate the effect of metformin on osteoclast differentiation and apoptosis. Low concentration of metformin inhibited osteoclast differentiation and downregulated the expression of TRAP, RANK, Cathepsink, NFATC-1, MMP-9 and TRAF-6. High concentration of metformin promoted osteoclast apoptosis and upregulated the expression of Bax/Bcl-2 and caspase-3; BV/TV, BS/TV, Tb.N and BMD were increased while Tp.Sp decreased in the group of intraperitoneal metformin+femoral intramedullary osteoclast injection (Met+OC) compared with the control group, 1 nM metformin downregulated Akt, p44/42 MAPK, JNK, p38 MAPK phosphorylation, 5 nM metformin down regulated ERK and Akt phosphorylation. These results suggest that a low concentration of metformin inhibits osteoclast differentiation through PI3K/Akt and MAPK/ERK signaling pathway; high concentrations of metformin promote osteoclast apoptosis through PI3K/Akt and ERK signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1691/ph.2021.1378DOI Listing
June 2021

The trans-ancestral genomic architecture of glycemic traits.

Nat Genet 2021 Jun 31;53(6):840-860. Epub 2021 May 31.

Department of Epidemiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.

Glycemic traits are used to diagnose and monitor type 2 diabetes and cardiometabolic health. To date, most genetic studies of glycemic traits have focused on individuals of European ancestry. Here we aggregated genome-wide association studies comprising up to 281,416 individuals without diabetes (30% non-European ancestry) for whom fasting glucose, 2-h glucose after an oral glucose challenge, glycated hemoglobin and fasting insulin data were available. Trans-ancestry and single-ancestry meta-analyses identified 242 loci (99 novel; P < 5 × 10), 80% of which had no significant evidence of between-ancestry heterogeneity. Analyses restricted to individuals of European ancestry with equivalent sample size would have led to 24 fewer new loci. Compared with single-ancestry analyses, equivalent-sized trans-ancestry fine-mapping reduced the number of estimated variants in 99% credible sets by a median of 37.5%. Genomic-feature, gene-expression and gene-set analyses revealed distinct biological signatures for each trait, highlighting different underlying biological pathways. Our results increase our understanding of diabetes pathophysiology by using trans-ancestry studies for improved power and resolution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41588-021-00852-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610958PMC
June 2021

Association of heart rate and diabetes among 0.5 million adults in the China Kadoorie biobank: Results from observational and Mendelian randomization analyses.

Nutr Metab Cardiovasc Dis 2021 Apr 27. Epub 2021 Apr 27.

Department of Epidemiology and Biostatistics, School of Public Health, Peking University Health Science Center, Beijing, China; Peking University Center for Public Health and Epidemic Preparedness & Response, Beijing, China. Electronic address:

Background And Aims: Observational studies have associated resting heart rate with incident diabetes. Whether the associations are causal remains unclear. We aimed to examine the shape and strength of the associations and assessed the causal relevance of such associations in Chinese adults.

Methods And Results: The China Kadoorie Biobank enrolled 512,891 adults in China. Cox proportional hazard regression models was conducted to estimate hazard ratios (HRs) for the associations of resting heart rate with type 2 diabetes and total diabetes. Among 92,724 participants, 36 single-nucleotide polymorphisms (SNPs) related to resting heart rate were used to construct genetic risk score. We used Mendelian randomization analyses to make the causal inferences. During a median follow-up of 9 years, 7872 incident type 2 diabetes and 13,349 incident total diabetes were documented. After regression dilution bias adjustment, each 10 bpm higher heart rate was associated with about a 26% higher risk of type 2 diabetes (HR, 1.26 [95% CI, 1.23, 1.29]) and 23% higher risk of total diabetes (HR, 1.23 [95% CI, 1.20, 1.26]). Instrumental variable analyses showed participants at top quintile compared with those at bottom quintile had 30% higher risk for type 2 diabetes (HR, 1.30 [95% CI, 1.17, 1.43]), and 10% higher risk for total diabetes (HR, 1.10 [95% CI, 1.02, 1.20]).

Conclusions: This study provides evidence that resting heart rate is an important risk factor for diabetes risk. The results suggest that novel treatment approaches targeting reduction of high heart rate for incidence of diabetes may be worth further investigation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.numecd.2021.04.015DOI Listing
April 2021

Overview of Antabuse (Disulfiram) in Radiation and Cancer Biology.

Authors:
Yaqi Jia Tao Huang

Cancer Manag Res 2021 20;13:4095-4101. Epub 2021 May 20.

Department of Hepatobiliary Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.

Antabuse, generic name disulfiram, has been extensively used in daily clinical practice to treat alcohol abuse. In vivo and in vitro experiments have demonstrated that disulfiram was capable of inhibiting tumor cell proliferation; clinical studies have indicated that the administration of this drug was associated with favorable survival, whilst in vitro experiments have elucidated the anticancer mechanism of disulfiram. In addition, radiation and cancer biology studies have shown that disulfiram can protect normal cells and sensitize tumor cells during radiotherapy. This review aims at describing the antitumor activity of disulfiram in both preclinical studies and clinical trials, whilst focusing on the advances of this drug in radiation and cancer biology, and the promise of repurposing it as a novel sensitizer to, and protector against, radiation on the incoming clinical studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/CMAR.S308168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146747PMC
May 2021

Ribosomal protein S26 serves as a checkpoint of T-cell survival and homeostasis in a p53-dependent manner.

Cell Mol Immunol 2021 May 25. Epub 2021 May 25.

Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Immunology, School of Basic Medical Science, Shandong University, Jinan, Shandong, PR China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41423-021-00699-4DOI Listing
May 2021

Identification of Key Genes With Differential Correlations in Lung Adenocarcinoma.

Front Cell Dev Biol 2021 5;9:675438. Epub 2021 May 5.

Tumor Biological Diagnosis and Treatment Center, The Third Affiliated Hospital of Soochow University, Changzhou, China.

Background: With the advent of large-scale molecular profiling, an increasing number of oncogenic drivers contributing to precise medicine and reshaping classification of lung adenocarcinoma (LUAD) have been identified. However, only a minority of patients archived improved outcome under current standard therapies because of the dynamic mutational spectrum, which required expanding susceptible gene libraries. Accumulating evidence has witnessed that understanding gene regulatory networks as well as their changing processes was helpful in identifying core genes which acted as master regulators during carcinogenesis. The present study aimed at identifying key genes with differential correlations between normal and tumor status.

Methods: Weighted gene co-expression network analysis (WGCNA) was employed to build a gene interaction network using the expression profile of LUAD from The Cancer Genome Atlas (TCGA). R package DiffCorr was implemented for the identification of differential correlations between tumor and adjacent normal tissues. STRING and Cytoscape were used for the construction and visualization of biological networks.

Results: A total of 176 modules were detected in the network, among which yellow and medium orchid modules showed the most significant associations with LUAD. Then genes in these two modules were further chosen to evaluate their differential correlations. Finally, dozens of novel genes with opposite correlations including ATP13A4-AS1, HIGD1B, DAP3, and ISG20L2 were identified. Further biological and survival analyses highlighted their potential values in the diagnosis and treatment of LUAD. Moreover, real-time qPCR confirmed the expression patterns of ATP13A4-AS1, HIGD1B, DAP3, and ISG20L2 in LUAD tissues and cell lines.

Conclusion: Our study provided new insights into the gene regulatory mechanisms during transition from normal to tumor, pioneering a network-based algorithm in the application of tumor etiology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.675438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131847PMC
May 2021

Physical activity and amyotrophic lateral sclerosis: a Mendelian randomization study.

Neurobiol Aging 2021 Apr 22. Epub 2021 Apr 22.

Department of Neurology, Peking University Third Hospital, Beijing, China; Beijing Municipal Key Laboratory of Biomarker and Translational Research in Neurodegenerative Diseases, Beijing, China. Electronic address:

Physical activity (PA) participation has been noted as a potential risk factor for amyotrophic lateral sclerosis (ALS) for decades. However, current studies have been unable to pinpoint the exact relationship between them. Here, we used 2-sample Mendelian randomization (MR), a novel method to systematically investigate causal relationships between PA and ALS. Summary-level data for accelerometer-based and self-reported PA phenotypes were obtained from 2 large genome-wide association studies (GWASs; n = 91,105-377,234), and the ALS summary statistics were from a GWAS of 20,806 cases and 59,804 healthy participants. The present MR study affords no support for causality between 5 included activity habits and ALS. We conclude that PA in the general population is unlikely to affect ALS incidence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neurobiolaging.2021.04.010DOI Listing
April 2021

Effects of chitosan-sodium alginate-nisin preservatives on the quality and spoilage microbiota of Penaeus vannamei shrimp during cold storage.

Int J Food Microbiol 2021 Jul 4;349:109227. Epub 2021 May 4.

College of Food and Pharmaceutical Science, Ningbo University, Ningbo, Zhejiang 315800, China. Electronic address:

The present work mainly investigated the effects of prepared chitosan‑sodium alginate-nisin (CS-SA-N) preservatives on the quality and bacterial phase of Penaeus vannamei shrimp during cold storage. Results showed that CS-SA-N preservatives treated samples had the lower pH, total volatile basic nitrogen (TVB-N), total viable count (TVC), and freeness (K) values than those of untreated ones during cold storage. The sensory evaluation results indicated that CS-SA-N preservatives treated shrimps had the higher comprehensive scores than those of untreated ones during whole storage. Microbial community of all samples was dominated by Proteobacteria. The initial predominant bacteria of fresh shrimps were Sphingomonas, Carnobacterium and Psychrobacter. Psychrobacter, Pseudomonas, and Shewanella, Acinetobacter and Vibrio were the predominant bacteria of untreated samples. CS-SA-N preservatives significantly decreased predominant microbial numbers by inhibiting the growth of Psychrobacter, Vibrio, Acinetobacter and Carnobacterium during cold storage. Therefore, the CS-SA-N preservatives could be used to prolong the shelf life of shrimp and guarantee its quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijfoodmicro.2021.109227DOI Listing
July 2021

Hepatic stellate cells-specific LOXL1 deficiency abrogates hepatic inflammation, fibrosis, and corrects lipid metabolic abnormalities in non-obese NASH mice.

Hepatol Int 2021 May 20. Epub 2021 May 20.

Liver Research Center, Beijing Key Laboratory of Translational Medicine in Liver Cirrhosis, Beijing Friendship Hospital, Capital Medical University, No. 95 Yong'an Road, Xicheng District, Beijing, 100050, People's Republic of China.

Background And Aims: Lysyl oxidase-like-1 (LOXL1), a vital cross-linking enzyme in extracellular matrix (ECM) maintenance, promotes fibrosis via enhancement of ECM stability. However, the potential role of LOXL1 in the pathogenesis of nonalcoholic steatohepatitis (NASH) has not been previously studied.

Methods: We generated Loxl1 mice to selectively delete LOXL1 in hepatic stellate cells (HSCs) (Loxl1Gfap; Loxl1 as littermate controls) and then examined liver pathology and metabolic profiles in Loxl1Gfap fed with either a choline-deficient L-amino acid-defined (CDAA) diet or an isocaloric control diet for 16 weeks. Thereafter, the findings from the animal model were confirmed in 23 patients with biopsy-proven non-alcoholic fatty liver disease (NAFLD).

Results: LOXL1 was significantly increased in CDAA induced non-obese NASH compared with the control diet, and LOXL1 deficient in HSCs ameliorated CDAA-induced inflammation and fibrosis, with reduced expression of pro-inflammation and pro-fibrogenic genes in the HSCs-specific LOXL1 knockout mice model. Interestingly, LOXL1 deficient in HSCs could attenuate hepatic steatosis and reverse the metabolic disorder by restoring adipose tissue function without altering the effect of hepatic lipogenesis gene expression in non-obese NASH model. More importantly, analyses of serum LOXL1 and leptin levels from NAFLD patients revealed that LOXL1 was positively correlated with histological fibrosis progression, whereas it was inversely correlated with leptin levels, especially in non-obese NAFLD patients.

Conclusion: LOXL1 may contribute to fibrosis progression in non-obese NAFLD, and HSCs-specific knockout of LOXL1 attenuated liver steatosis, inflammation, fibrosis, , and improved lipid metabolic abnormalities. Hence, LOXL1 inhibition may serve as a new therapeutic strategy for NASH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12072-021-10210-wDOI Listing
May 2021

A further look at quantitative trait loci for growth and fatness traits in a White Duroc × Erhualian F intercross population.

Anim Biotechnol 2021 May 19:1-12. Epub 2021 May 19.

State Key Laboratory for Swine Genetic Improvement and Production Technology, Jiangxi Agricultural University, Nanchang, China.

Genetic analysis of porcine growth and fatness traits is beneficial to the swine industry and provides a reference to understand human obesity. Here, we obtained 29 growth and fatness traits for 473 individuals from a White Duroc × Erhualian F intercross population. Basic statistical analyses showed that: (1) Positive correlations between different-stage body weights were detected, the shorter the time interval the stronger the correlation. (2) Strong correlations existed in the paired fatness traits. (3) With the growth of age, the correlation between fatness and body weight was increasing. All pigs were genotyped by Illumina 50 K SNP chips and their whole-genome genotypes were imputed referred to 109 re-sequencing data. We performed common and imputation-based GWASs for these traits. Two genome-wide significant loci on swine chromosome (SSC) 4 and 7 were repeatedly detected. The strongest association ( = 3.24 × 10) was detected at 31.96 Mb on SSC7 for leaf fat weight. On this locus, seven major haplotypes were identified, of which two were novel and had an increasing-fatness effect. In the imputation-based GWAS, three new loci were identified. Our findings provide further insights into and enhance our understanding of genetic mechanism of porcine growth and fat deposition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10495398.2021.1884087DOI Listing
May 2021

Genomic variation, origin tracing, and vaccine development of SARS-CoV-2: A systematic review.

Innovation (N Y) 2021 May 11;2(2):100116. Epub 2021 May 11.

Genetic Testing Center, Academician Workstation, Changsha Medical University, Changsha 410219, China.

COVID-19 has spread globally to over 200 countries with more than 40 million confirmed cases and one million deaths as of November 1, 2020. The SARS-CoV-2 virus, leading to COVID-19, shows extremely high rates of infectivity and replication, and can result in pneumonia, acute respiratory distress, or even mortality. SARS-CoV-2 has been found to continue to rapidly evolve, with several genomic variants emerging in different regions throughout the world. In addition, despite intensive study of the spike protein, its origin, and molecular mechanisms in mediating host invasion are still only partially resolved. Finally, the repertoire of drugs for COVID-19 treatment is still limited, with several candidates still under clinical trial and no effective therapeutic yet reported. Although vaccines based on either DNA/mRNA or protein have been deployed, their efficacy against emerging variants requires ongoing study, with multivalent vaccines supplanting the first-generation vaccines due to their low efficacy against new strains. Here, we provide a systematic review of studies on the epidemiology, immunological pathogenesis, molecular mechanisms, and structural biology, as well as approaches for drug or vaccine development for SARS-CoV-2.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.xinn.2021.100116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110321PMC
May 2021

Identifying protein subcellular locations with embeddings-based node2loc.

IEEE/ACM Trans Comput Biol Bioinform 2021 May 14;PP. Epub 2021 May 14.

Identifying protein subcellular locations is an important topic in protein function prediction. Interacting proteins may share similar locations. Thus, it is imperative to infer protein subcellular locations by taking protein-protein interactions (PPIs) into account. In this study, we present a network embedding-based method, node2loc, to identify protein subcellular locations. node2loc first learns distributed embeddings of proteins in a protein-protein interaction (PPI) network using node2vec. Then the learned embeddings are further fed into a recurrent neural network (RNN). To resolve the severe class imbalance of different subcellular locations, Synthetic Minority Over-sampling Technique (SMOTE) is applied to artificially synthesize proteins for minority classes. node2loc is evaluated on our constructed human benchmark dataset with 16 subcellular locations and yields a Matthews correlation coefficient (MCC) value of 0.800, which is superior to baseline methods. In addition, node2loc yields a better performance on a Yeast benchmark dataset with 17 locations. The results demonstrate that the learned representations from a PPI network have certain discriminative ability for classifying protein subcellular locations. However, node2loc is a transductive method, it only works for proteins connected in a PPI network, and it needs to be retrained for new proteins. In addition, the PPI network needs be annotated to some extent with location information.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TCBB.2021.3080386DOI Listing
May 2021

The Thickness of Surface Grafting Layer on Bio-materials directly Mediates the Immuno-reacitivity of Macrophages .

Open Life Sci 2020 3;15:198-208. Epub 2020 May 3.

Department of Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515 China.

Introducing the surface grafting layers to regulate bio-compatibility and bio-function is an important step in the tissue engineering field. However, whether the thickness variation of the introduced biomimetic layer impacts the behavior of the adhered immune effector cells is yet to be dissected fully. In this study, we used a surface-induced atom transfer radical polymerization (SI-ATRP) method to synthetize and graft poly-phenoxyethyl methacrylate (PHEMA) brushes having different lengths on the glass substrates. Primary murine peritoneal macrophages were collected and cultured on the PHEMA brushes and we investigated the influence of polymer brushes having different lengths on macrophages phenotype and function. Our results demonstrated that the thicker brushes (200 nm and 450 nm) are superior to the thinner layers (50 nm) for macrophages survival, proliferation, cell elongation and migration. Moreover, the thicker brushes are more beneficial for macrophage's activities and functions, presented by the increased production of M1-associated cytokines IL-6 and MCP-1, the elevated cell phagocytosis and the activation molecule F4/80 expression, and the reduced macrophages apoptosis in thicker brushes-sustained macrophages. Our data suggests that the thickness of the substrate grafting layer directly impacts macrophages recruitment and pro-inflammatory function, which is important in determining the intrinsic immuno-compatibilities of the surface modified-biomaterials and mediates material-host interactions .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1515/biol-2020-0020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114785PMC
May 2020

All-fiber rotation speed measurement based on dual-beam speckle interference.

Appl Opt 2021 May;60(14):4064-4067

An all-fiber rotation speed measurement structure based on dual-beam speckle interference is proposed. A distributed feedback laser as the light source generates two independent laser beams through a coupler and two circulators. Scattered by the object, the two laser beams interfere separately with the light reflected from the ends of the two fibers, which can be used to measure the rotation speed. The experiment results show that the structure can accurately measure the rotation speed of the object, with relative errors less than 0.5%.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/AO.418233DOI Listing
May 2021

Morphological characteristics and ecological risk assessment of nitrogen and phosphorus in the sediments of Futunxi watershed in Fujian province.

Environ Monit Assess 2021 May 10;193(6):335. Epub 2021 May 10.

School of Geography Science, Nanjing Normal University, Nanjing, 210023, Jiangsu, China.

The total amount and morphology of nitrogen and phosphorus in sediments have important environmental significance. The study of the ecological risk assessment of nitrogen and phosphorus in sediments is important to understand the environmental quality of water body in the basin. In this study, taking the Futunxi Basin of Fujian Province as an example, the single factor index and bioavailability coefficient method were used to evaluate the ecological risk of nitrogen and phosphorus in the sediments, and reveal the spatial change and environmental significance of the nitrogen and phosphorus forms. The results showed that different morphological components of the bio-available nitrogen were distributed as organic sulfide bound nitrogen (SOEF-N) > iron-manganese oxidized nitrogen (SAEF-N) > weak acid leached nitrogen (WAEF-N) > ion exchange nitrogen (IEF-N). The inorganic phosphorus included the highest proportion of metal oxide bound phosphorus (NaOH-P), followed by calcium bound phosphorus (HCl-P). The proportion of reduced phosphorus (BD-P) was even lower, and the proportion of weakly adsorbed phosphorus (NH4Cl-P) was the lowest. The results of single factor pollution index showed that the sediment nitrogen in the study area mainly caused moderate pollution and phosphorus mainly caused light pollution. By contrast, the results of the bioavailability index method indicated that nitrogen mainly caused light pollution, and phosphorus mainly caused clean pollution in sediments. Combined with the characteristics of social economy and environment in the study area, it can be inferred that the bioavailability index method based on comprehensive evaluation of total amount and morphology can better characterize the spatial change and ecological risk of nitrogen and phosphorus in sediments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10661-021-09106-xDOI Listing
May 2021

Single Hormone Receptor-Positive Metaplastic Breast Cancer: Similar Outcome as Triple-Negative Subtype.

Front Endocrinol (Lausanne) 2021 23;12:628939. Epub 2021 Apr 23.

Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Metaplastic breast cancer (MBC) is a rare and aggressive subtype of the breast. To understand the characteristics and prognosis of single hormone receptor-positive (HR+) MBC (estrogen receptor-positive [ER+]/progesterone receptor-negative [PR-] and ER-/PR+), we compared these tumors to double HR+ tumors as well as HR- tumors.

Patients And Methods: The Surveillance, Epidemiology, and End Results database was used to analyze MBC between 1975 and 2016. The effect of HR status was evaluated using a multivariate Cox regression model.

Results: We included 3369 patients with a median follow-up time of 42 months (range 0-322 months). In this study, 280 (8.3%) cases were double HR+ tumors, 2597 (77.1%) were double HR- tumors, and 492 (14.6%) cases were single HR+ tumors, of which 159 (4.7%) cases were ER-/PR+ tumors and 333 (9.9%) were ER+/PR- tumors. On multivariate Cox analysis, the prognosis was related to age, race/ethnicity, tumor grade, TNM stage, and surgery. HR status remained no impact on breast cancer-specific survival (BCSS). In the Kaplan-Meier curve, HR status was not associated with better BCSS or overall survival (OS). In patients without HER2 overexpression, the BCSS and OS of ER+/PR- and ER-/PR+ tumors were not significantly different from that of ER-/PR- and ER+/PR+ tumors. The difference remains no significant in patients with HER2 overexpression.

Conclusions: In comparison with both ER-/PR- and ER+/PR+ tumors, we have identified clinically and biologically distinct features of single HR+ tumors. In patients with or without HER2 overexpression, the prognosis of single HR+ tumors was similar to ER-/PR- and ER+/PR+ tumors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2021.628939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105402PMC
April 2021

Editorial: Computational Methods in Predicting Complex Disease Associated Genes and Environmental Factors.

Front Genet 2021 21;12:679651. Epub 2021 Apr 21.

Medical and Population Genetics Program, Broad Institute of MIT and Harvard, Cambridge, MA, United States.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.679651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097088PMC
April 2021

Male reproductive systems of Macaca mulatta: Gonadal development, spermatogenesis and applications in spermatogonia stem cell transplantation.

Res Vet Sci 2021 Jul 4;137:127-137. Epub 2021 May 4.

Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350001, China; Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, National Health Commission, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350013, China. Electronic address:

Rhesus macaque (Macaca mulatta) is widely applied in animal model construction of infertility, spermatogonia stem cell transplantation and male reproductive diseases. In this review, we describe the seasonal changes of the reproductive system in rhesus macaques, the regular pattern of spermatogenesis and spermatozoa maturation, and the differentiation of spermatogonia and spermatocytes. The duration of the M. mulatta spermatogenesis is approximately 10 days and seminiferous epithelium cycles mainly consist of 12 stages, which provide a suitable model for reproductive studies in non-human primates. Here, we summarize the features of gonadal development and sperm maturation in the rhesus monkeys, which provide important information in the studies of reproductive biology. Rhesus macaque is an excellent animal model in spermatogonia stem cell transplantation. We discuss the applications and progresses of assisted reproductive technologies in sperm liquefaction, semen cryopreservation and spermatogonia stem cell transplantation of rhesus macaques. Besides, we sort out recent proteomic analyses of male reproductive systems and semen samples in rhesus macaques. This review mainly focuses on male reproductive biology and application studies using M. mulatta, which would promote the development of new therapeutic interventions on assisted reproduction and reproductive disease studies in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.rvsc.2021.04.033DOI Listing
July 2021

Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals.

Nat Commun 2021 05 7;12(1):2563. Epub 2021 May 7.

National Centre for Gene Research, CAS-JIC Centre of Excellence for Plant and Microbial Science (CEPAMS), Centre of Excellence for Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS), Shanghai, China.

Non-random gene organization in eukaryotes plays a significant role in genome evolution. Here, we investigate the origin of a biosynthetic gene cluster for production of defence compounds in oat-the avenacin cluster. We elucidate the structure and organisation of this 12-gene cluster, characterise the last two missing pathway steps, and reconstitute the entire pathway in tobacco by transient expression. We show that the cluster has formed de novo since the divergence of oats in a subtelomeric region of the genome that lacks homology with other grasses, and that gene order is approximately colinear with the biosynthetic pathway. We speculate that the positioning of the late pathway genes furthest away from the telomere may mitigate against a 'self-poisoning' scenario in which toxic intermediates accumulate as a result of telomeric gene deletions. Our investigations reveal a striking example of adaptive evolution underpinned by remarkable genome plasticity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-22920-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105312PMC
May 2021

Proton-controlled non-exponential photoluminescence in a pyridylamidine-substituted Re(I) complex.

Dalton Trans 2021 Jun;50(21):7265-7276

Department of Chemistry, Lehigh University, Bethlehem, Pennsylvania 18015, USA.

Chemical intuition and well-known design principles can typically be used to create ligand environments in transition metal complexes to deliberately tune reactivity for desired applications. However, intelligent ligand design does not always result in the expected outcomes. Herein we report the synthesis and characterization of a tricarbonyl rhenium (2,2'-bipyridine) 4-pyridylamidine, Re(4-Pam), complex with unexpected photophysical properties. Photoluminescence kinetics of Re(4-Pam) undergoes non-exponential decay, which can be deconvolved into two emission lifetimes. However, upon protonation of the amidine functionality of the 4-pyridylamidine to form Re(4-PamH), a single exponential decay is observed. To understand and rationalize these experimental observations, density functional theory (DFT) and time-dependent density functional theory (TDDFT) are employed. The symmetry or asymmetry of the protonated or deprotonated 4-pyridylamidine ligand, respectively, is the key factor in switching between one and two photoluminescence lifetimes. Specifically, rotation of the dihedral angle formed between the bipyridine and 4-Pam ligand leads to two rotamers of Re(4-Pam) with degenerate triplet- to ground-state transitions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1dt01132dDOI Listing
June 2021

Corrigendum to "The Antiosteoporosis Effects of Yishen Bugu Ye Based on Its Regulation on the Differentiation of Osteoblast and Osteoclast".

Biomed Res Int 2021 12;2021:9824165. Epub 2021 Apr 12.

Department of Orthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China.

[This corrects the article DOI: 10.1155/2020/9467683.].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/9824165DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068548PMC
April 2021

MYC/NBS1-Mediated DNA Damage Response is Involved in the Inhibitory Effect of Hydroxysafflor Yellow A on Glioma Cells.

Drug Des Devel Ther 2021 28;15:1749-1763. Epub 2021 Apr 28.

Department of Neurosurgery, The Second Affiliated Hospital of Air Force Military Medical University, Xi'an City, People's Republic of China.

Background: The role of Hydroxysafflor Yellow A (HSYA) in glioma is less studied, this research determined the effect of HSYA on glioma cells.

Methods: The expressions of MYC and NBS1 in glioma tissues were detected by bioinformatics analysis and verified by RT-qPCR. The target relationship between MYC and NBS1 was predicted by bioinformatics. After treating the cells with HSYA, silenced MYC, or overexpressed NBS1, the viability, apoptosis, proliferation, invasion, migration, and DNA damage of the glioma cells were detected by MTT, flow cytometry, colony formation, transwell, wound healing, and γH2AX immunofluorescence assays, respectively. IC of HSYA in glioma cells was analyzed by Probit regression analysis. The expressions of MYC, NBS1, factors related to migration, invasion, apoptosis, and DNA damage of the glioma cells were determined by Western blot or RT-qPCR.

Results: MYC and NBS1 were high-expressed in glioma, and NBS1 was targeted by MYC. HSYA and siRNA targeting MYC inhibited the cell viability, proliferation, invasion, migration, and induced the cell apoptosis of glioma cells. HSYA upregulated the expressions of MYC, γH2AX, E-Cadherin, Bax, and Cleaved-PARP1, stimulated the activation of NBS1, MRE11, RAD50, and ATM, and downregulated the expressions of N-Cadherin and Bcl2 in glioma cells. SiMYC decreased the IC of HSYA in the glioma cells, enhanced the sensitivity of glioma cells to HSYA, and inhibited the activation of NBS1 and ATM. NBS1 overexpression reversed the effect of siRNA targeting MYC on glioma cells.

Conclusion: MYC silencing inhibited the DNA damage response via regulation of NBS1, leading to DNA repair deficiency, and subsequently enhanced the sensitivity of glioma cells to HSYA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/DDDT.S288841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8089107PMC
April 2021

Assessment of causal association between thyroid function and lipid metabolism: a Mendelian randomization study.

Chin Med J (Engl) 2021 Apr 13;134(9):1064-1069. Epub 2021 Apr 13.

Department of Cardiology, Peking University Third Hospital, Beijing 100191, China.

Background: Thyroid dysfunction is associated with cardiovascular diseases. However, the role of thyroid function in lipid metabolism remains partly unknown. The present study aimed to investigate the causal association between thyroid function and serum lipid metabolism via a genetic analysis termed Mendelian randomization (MR).

Methods: The MR approach uses a genetic variant as the instrumental variable in epidemiological studies to mimic a randomized controlled trial. A two-sample MR was performed to assess the causal association, using summary statistics from the Atrial Fibrillation Genetics Consortium (n = 537,409) and the Global Lipids Genetics Consortium (n = 188,577). The clinical measures of thyroid function include thyrotropin (TSH), free triiodothyronine (FT3) and free thyroxine (FT4) levels, FT3:FT4 ratio and concentration of thyroid peroxidase antibodies (TPOAb). The serum lipid metabolism traits include total cholesterol (TC) and triglycerides, high-density lipoprotein, and low-density lipoprotein (LDL) levels. The MR estimate and MR inverse variance-weighted method were used to assess the association between thyroid function and serum lipid metabolism.

Results: The results demonstrated that increased TSH levels were significantly associated with higher TC (β = 0.052, P = 0.002) and LDL (β = 0.041, P = 0.018) levels. In addition, the FT3:FT4 ratio was significantly associated with TC (β = 0.240, P = 0.033) and LDL (β = 0.025, P = 0.027) levels. However, no significant differences were observed between genetically predicted FT4 and TPOAb and serum lipids.

Conclusion: Taken together, the results of the present study suggest an association between thyroid function and serum lipid metabolism, highlighting the importance of the pituitary-thyroid-cardiac axis in dyslipidemia susceptibility.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/CM9.0000000000001505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116035PMC
April 2021

RO4929097 regulates RANKL-induced osteoclast formation and LPS-mediated bone resorption.

Aging (Albany NY) 2021 05 2;13(9):12526-12536. Epub 2021 May 2.

Department of Orthopaedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

To investigate the suppressive function of RO4929097, a potent -secretase inhibitor, on RANKL-induced osteoclastogenesis. The cytotoxicity of RO4929097 was evaluated. The suppressive effect and possible molecular mechanism of RO4929097 on RANKL-induced osteoclastogenesis was evaluated both and . The IC50 of RO4929097 was 2.93 μM. Treatment with different doses of RO4929097 (100 nM, 200 nM, and 400 nM) effectively reduced osteoclast formation (number and resorption area) in a dose-dependent manner. The qPCR results revealed that RO4929097 attenuates RANKL-induced osteoclast formation and NFATc1 protein expression. The experiments demonstrated that RO4929097 had an inhibitory effect on LPS-induced bone resorption. Our experiments showed that RO4929097 can potently inhibit osteoclastogenesis and bone resorption by down-regulating the Notch/MAPK/JNK/Akt-mediated reduction of NFATc1. In accordance with these observations, RO4929097 attenuated LPS-induced osteolysis in mice. In conclusion, our findings indicate that Notch may represent a potential therapeutic target for the treatment of osteolytic diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.202926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148457PMC
May 2021