Publications by authors named "Tao He"

909 Publications

Detecting Rotated Objects as Gaussian Distributions and Its 3-D Generalization.

IEEE Trans Pattern Anal Mach Intell 2022 Aug 8;PP. Epub 2022 Aug 8.

Existing detection methods commonly use a parameterized bounding box (BBox) to model and detect (horizontal) objects and an additional rotation angle parameter is used for rotated objects. We argue that such a mechanism has fundamental limitations in building an effective regression loss for rotation detection, especially for high-precision detection with high IoU (e.g. 0.75). Instead, we propose to model the rotated objects as Gaussian distributions. A direct advantage is that our new regression loss regarding the distance between two Gaussians e.g. Kullback-Leibler Divergence (KLD), can well align the actual detection performance metric, which is not well addressed in existing methods. Moreover, the two bottlenecks i.e. boundary discontinuity and square-like problem also disappear. We also propose an efficient Gaussian metric-based label assignment strategy to further boost the performance. Interestingly, by analyzing the BBox parameters' gradients under our Gaussian-based KLD loss, we show that these parameters are dynamically updated with interpretable physical meaning, which help explain the effectiveness of our approach, especially for high-precision detection. We extend our approach from 2-D to 3-D with a tailored algorithm design to handle the heading estimation, and experimental results on twelve public datasets (2-D/3-D, aerial/text/face images) with various base detectors show its superiority.
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http://dx.doi.org/10.1109/TPAMI.2022.3197152DOI Listing
August 2022

Clinical Effect of Microneedle Injection Combined with Blood Transfusion in the Treatment of Severe Anemia Complicated with Vitiligo under Regenerative Medical Technology.

Authors:
Tao He Li Gong

Biomed Res Int 2022 29;2022:7117627. Epub 2022 Jul 29.

Department of Dermatology, The First Hospital of Heilongjiang Harbin, Harbin, 150010 Heilongjiang, China.

To explore the clinical efficacy of microneedle injection combined with blood transfusion in the treatment of severe anemia complicated with vitiligo based on regenerative medical technology and provide the theoretical basis for the adoption of microneedle technology, 60 patients with severe anemia complicated with vitiligo were selected as research objects. With 15 patients in each group, they were randomly assigned to the control group (calcipotriol ointment external application), observation group A (external application of moist exposed burn ointment (MEBO), observation group B (external application of MEBO combined with blood transfusion), and observation group C (microneedle injection of MEBO combined with blood transfusion). Blood indexes and plaque recovery of patients in different periods were detected. The total protein (TP) content in group C (62.3 ± 3.3 g/L and 64.3 ± 2.88 g/L) was remarkably higher than that in the control group (51.3 ± 3.17 g/L and 52.4 ± 3.17 g/L) and group A (52.6 ± 2.91 g/L and 51.8 ± 2.98 g/L)) at the 5th and 7th weeks after the treatment ( < 0.05). The albumin (ALB) content in group C (42.9 ± 3.28 g/L and 45.3 ± 3.1 g/L) was signally higher than that in the control group (41.8 ± 3.44 g/L and 41.9 ± 3.23 g/L) and group A (41.3 ± 2.91 g/L and 42.1 ± 3.02 g/L) at the 5th and 7th weeks after the treatment, and the content was markedly higher than that in group B at 5th week ( < 0.05). The wound healing rates of group C at the 3rd, 5th, and 7th weeks after the treatment (38.44%, 56.6%, and 90.23%) were greatly higher than those of the control group, group A, and group B ( < 0.05). Besides, the wound healing rate of group B was higher than that of the control group and group A (40.3% and 75.8%) at the 5th and 7th weeks after the treatment ( < 0.05). To sum up, based on regenerative medical technology, microneedle injection (microneedling is a derma roller process that pricks the skin with minuscule needles. The goal of the treatment is to develop new collagen and skin tissue, resulting in skin that is smoother, firmer, and more toned) combined with blood transfusion had a good therapeutic effect on patients with severe anemia complicated with vitiligo, which could manifestly improve the blood indexes and skin plaques of patients, with a good clinical adoption effect.
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http://dx.doi.org/10.1155/2022/7117627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355759PMC
August 2022

The prognostic role of surgery and a nomogram to predict the survival of stage IV breast cancer patients.

Gland Surg 2022 Jul;11(7):1224-1239

Clinical Research Center for Breast Diseases, West China Hospital, Sichuan University, Chengdu, China.

Background: Since numerous retrospective studies and prospective trials have shown divergent results, whether the surgical excision of the primary tumor results in survival benefits for stage IV breast cancer patients is inconclusive. Consequently, we need a prediction model of prognosis, judge the efficiency of breast surgery, and identify the advanced breast cancer patients who would benefit from surgery.

Methods: We analyzed the data of 2,747 metastatic breast cancer patients (the surgery group) and 4,508 patients (the non-surgery group) from the Surveillance, Epidemiology, and End Results (SEER) database during 2010-2015. Propensity score matching (PSM) was used to attain a balance between the covariates of both groups. We then assessed the potential risk factors for the breast cancer-specific survival (BCSS) of patients in the non-surgery group by Cox regression and constructed a nomogram to predict BCSS. All the patients were classified into different risk groups based on the median risk score obtained from the nomogram. The hazard ratios of BCSS and overall survival (OS) of patients in the two groups were calculated.

Results: After PSM, 2,288 patients severally in the two groups (the surgery group and the non-surgery group) were enrolled in the study. A nomogram incorporating 13 potential risk factors (i.e., age, race, cohabitation status, income, tumor grade, histotype, tumor size, lymph node status, molecular subtype, metastasis to brain, liver, lung, and chemotherapy) was constructed using the data of patients in the non-surgery group. The C statistics for the internal (patients in the non-surgery group) and external (patients in the surgery group) validation of the nomogram were 0.70 [95% confidence interval (CI), 0.69-0.71] and 0.73 (95% CI, 0.72-0.74), respectively. In the low-risk group, patients in the surgery group had lower risks of breast cancer-specific mortality (BCSM) (hazard ratio =0.53; 95% CI, 0.47-0.59; P for interaction =0.014) and overall mortality (OM) (hazard ratio =0.52; 95% CI, 0.46-0.58; P for interaction =0.002) than those in the non-surgery group.

Conclusions: Breast surgery might improve the survival of metastatic breast cancer patients in the low-risk group. The established nomogram could provide a reference for clinicians in enabling personalized treatment among advanced breast cancer patients.
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http://dx.doi.org/10.21037/gs-22-330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346227PMC
July 2022

Correction: Knockout serotonin transporter in rats moderates outcome and stimulus generalization.

Transl Psychiatry 2022 Aug 5;12(1):315. Epub 2022 Aug 5.

Department of Cognitive Neuroscience, Donders Institute for Brain, Cognition, and Behaviour, Radboud University Medical Centre, Nijmegen, The Netherlands.

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http://dx.doi.org/10.1038/s41398-022-02098-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356137PMC
August 2022

The robust UCATR algorithm enhances the specificity and sensitivity to detect the infarct of acute ischaemic stroke within 6 hours of onset via non-contrast computed tomography images.

BMC Neurol 2022 Aug 4;22(1):291. Epub 2022 Aug 4.

Department of Public Health, Chengdu Medical College, Sichuan, 610500, China.

Problem Background: Early detection of acute ischemic stroke (AIS) may provide patients with benefits against harmful health and financial impacts. The use of non-contrast computed tomography images for early detect of the infarct remains controversial.

Materials & Methods: Here, we used the UCATR algorithm to extract the pixel values of the infarct and the corresponding contralateral healthy area as the control surface in each NCCT slice for the whole brain. Magnetic resonance imaging results were used to verify both areas. We found significant pathological changes in the infarct compared with the corresponding contralateral healthy area in each NCCT slice.

Attained Results: Our approach validated that NCCT can be used to detect the lesion area in the early stage of AIS.

Conclusions: With obvious advantages such as saving time and the ability to quantify the infarct volume, this approach could help more patients survive the fatal and irreversible pathological process of AIS and improve their quality of life after AIS treatment.
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http://dx.doi.org/10.1186/s12883-022-02825-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351169PMC
August 2022

Integrative Analyses Identify Potential Key Genes and Calcium-Signaling Pathway in Familial Atrioventricular Nodal Reentrant Tachycardia Using Whole-Exome Sequencing.

Front Cardiovasc Med 2022 18;9:910826. Epub 2022 Jul 18.

Department of Cardiology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Background: Atrioventricular nodal reentrant tachycardia (AVNRT) is a common arrhythmia. Growing evidence suggests that family aggregation and genetic factors are involved in AVNRT. However, in families with a history of AVNRT, disease-causing genes have not been reported.

Objective: To investigate the genetic contribution of familial AVNRT using a whole-exome sequencing (WES) approach.

Methods: Blood samples were collected from 20 patients from nine families with a history of AVNRT and 100 control participants, and we systematically analyzed mutation profiles using WES. Gene-based burden analysis, integration of previous sporadic AVNRT data, pedigree-based co-segregation, protein-protein interaction network analysis, single-cell RNA sequencing, and confirmation of animal phenotype were performed.

Results: Among 95 related reference genes, seven candidate pathogenic genes have been identified both in sporadic and familial AVNRT, including , , , , , , and . Among the 37 reference genes from sporadic AVNRT, five candidate pathogenic genes were identified in patients with both familial and sporadic AVNRT: , ryanodine receptor 2 (), , , and . To identify the common pathogenic mechanisms in all AVNRT cases, five pathogenic genes were identified in patients with both familial and sporadic AVNRT: , , , , and . Considering the unique internal candidate pathogenic gene within pedigrees, three genes, , , and , were likely to be the pathogenic genes in familial AVNRT. Notably, the core calcium-signaling pathway may be closely associated with the occurrence of AVNRT, including , , , , and .

Conclusion: Our pedigree-based studies demonstrate that and related calcium signaling pathway play a critical role in the pathogenesis of familial AVNRT using the WES approach.
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http://dx.doi.org/10.3389/fcvm.2022.910826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339905PMC
July 2022

Targeted inhibition of osteoclastogenesis reveals the pathogenesis and therapeutics of bone loss under sympathetic neurostress.

Int J Oral Sci 2022 Aug 1;14(1):39. Epub 2022 Aug 1.

State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, China.

Sympathetic cues via the adrenergic signaling critically regulate bone homeostasis and contribute to neurostress-induced bone loss, but the mechanisms and therapeutics remain incompletely elucidated. Here, we reveal an osteoclastogenesis-centered functionally important osteopenic pathogenesis under sympatho-adrenergic activation with characterized microRNA response and efficient therapeutics. We discovered that osteoclastic miR-21 was tightly regulated by sympatho-adrenergic cues downstream the β2-adrenergic receptor (βAR) signaling, critically modulated osteoclastogenesis in vivo by inhibiting programmed cell death 4 (Pdcd4), and mediated detrimental effects of both isoproterenol (ISO) and chronic variable stress (CVS) on bone. Intriguingly, without affecting osteoblastic bone formation, bone protection against ISO and CVS was sufficiently achieved by a (D-Asp)-lipid nanoparticle-mediated targeted inhibition of osteoclastic miR-21 or by clinically relevant drugs to suppress osteoclastogenesis. Collectively, these results unravel a previously underdetermined molecular and functional paradigm that osteoclastogenesis crucially contributes to sympatho-adrenergic regulation of bone and establish multiple targeted therapeutic strategies to counteract osteopenias under stresses.
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http://dx.doi.org/10.1038/s41368-022-00193-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343357PMC
August 2022

The diagnostic value of PET/CT for the lymph node metastasis in Asian patients with non-small cell lung cancer: A meta-analysis.

Hell J Nucl Med 2022 Aug 3. Epub 2022 Aug 3.

Department of Nuclear Medicine,Panzhihua Central Hospital, No.34 Yikang Street, Panzhihua,Sichuan 617067,China.

Objective: To evaluate the accuracy of positron emission tomography/computed tomography (PET/CT) in the diagnosis of lymph node metastasis in non-small cell lung cancer (NSCLC) by a method of meta-analysis.

Materials And Methods: A comprehensive search of PubMed, Cochrane Library, China National Knowledge Infrastructure (CNKI), and Embase data bases was conducted to collect literature about PET/CT diagnosing lymph node metastasis of NSCLC up to December 1, 2021. Stata 15.0 software was used for the calculation of sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR). The publication bias was evaluated by Deeks' funnel plot.

Results: A total of 25 studies were enrolled, including 2,458 patients with NSCLC. The pooled sensitivity of PET/CT for diagnosing lymph node metastasis in NSCLC was 0.68 (95%CI: 0.61-0.75), the pooled specificity being 0.93 (95%CI: 0.89-0.95). Likelihood ratio syntheses gave an overall PLR of 9.4 (95%CI: 6.3-13.9), and NLR of 0.34 (95%CI: 0.28-0.41). The pooled DOR was 28 (95%CI: 19-40). The summary receiver operating characteristic curve showed the area under the curve of 0.88 (95%CI: 0.84-0.90).

Conclusion: Positron emission tomography/CT has a good value in the diagnosis of lymph node metastasis of NSCLC, with specificity excellent. Positron emission tomography/CT can be used as one of the main imaging diagnosis methods for lymph node metastasis in patients with NSCLC.
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http://dx.doi.org/10.1967/s002449912479DOI Listing
August 2022

Intensity-surged and bandwidth-extended terahertz radiation in two-foci cascading plasmas.

Opt Lett 2022 Aug;47(15):3816-3819

The two-color strong-field mixing in gas medium is a widely used approach to generate bright broadband terahertz (THz) radiation. Here, we present a new, to the best of our knowledge, and counterintuitive method to promote THz performance in the two-color scheme. Beyond our knowledge that the maximum THz generation occurs with two-color foci overlapped, we found that, when the foci of two-color beams are noticeably separated along the propagation axis resulting in cascading plasmas, the THz conversion efficiency is surged by one order of magnitude and the bandwidth is stretched by more than two times, achieving 10 conversion efficiency and >100 THz bandwidth under the condition of 800/400 nm, ∼35 fs driving lasers. With the help of the pulse propagation equation and photocurrent model, the observations can be partially understood by the compromise between THz generation and absorption due to the spatial redistribution of laser energy in cascading plasmas. The present method can be extended to a mid-infrared driving laser, and new records of THz peak power and conversion efficiency are expected.
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http://dx.doi.org/10.1364/OL.465496DOI Listing
August 2022

PRIM2 Promotes Cell Cycle and Tumor Progression in p53-Mutant Lung Cancer.

Cancers (Basel) 2022 Jul 11;14(14). Epub 2022 Jul 11.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, China.

p53 is a common tumor suppressor, and its mutation drives tumorigenesis. What is more, p53 mutations have also been reported to be indicative of poor prognosis in lung cancer, but the detailed mechanism has not been elucidated. In this study, we found that DNA primase subunit 2 (PRIM2) had a high expression level and associated with poor prognosis in lung cancer. Furthermore, we found that PRIM2 expression was abnormally increased in lung cancer cells with p53 mutation or altered the p53/RB pathway based on database. We also verified that PRIM2 expression was elevated by mutation or deletion of p53 in lung cancer cell lines. Lastly, silence p53 increased the expression of RPIM2. Thus, these data suggest that PRIM2 is a cancer-promoting factor which is regulated by the p53/RB pathway. The p53 tumor-suppressor gene integrates numerous signals that control cell proliferation, cell cycle, and cell death; and the p53/RB pathway determines the cellular localization of transcription factor E2F, which regulates the expression of downstream targets. Next, we explored the role of PRIM2 in lung cancer and found that knockdown of PRIM2 induced cell cycle arrest, increased DNA damage, and increased cell senescence, leading to decreased lung cancer cell proliferation. Lastly, the positive correlation between PRIM2 and E2F/CDK also indicated that PRIM2 was involved in promoting cell cycle mediated by p53/RB pathway. These results confirmed that the expression of PRIM2 is regulated by the p53/RB pathway in lung cancer cells, promotes DNA replication and mismatch repair, and activates the cell cycle. Overall, we found that frequent p53 mutations increased PRIM2 expression, activated the cell cycle, and promoted lung cancer progression.
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http://dx.doi.org/10.3390/cancers14143370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320259PMC
July 2022

Comparing the efficacy and safety of direct oral anticoagulants versus Vitamin K antagonists in patients with antiphospholipid syndrome: a systematic review and meta-analysis.

Blood Coagul Fibrinolysis 2022 Jul 22. Epub 2022 Jul 22.

Department of Vascular Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Thromboprophylaxis is the cornerstone strategy for thrombotic antiphospholipid syndrome (APS). Data comparing direct oral anticoagulants (DOACs) to Vitamin K antagonists (VKAs) in the secondary prevention of thrombosis in APS patients remain contentious. We aim to review and analyse literature on the efficacy and safety of DOACs compared with VKAs in treating patients with APS. A literature search was performed from inception to 31 December 2021. Subgroups were analysed based on the risk stratification of APS profiles and different DOAC types. A total of nine studies with 1131 patients were included in the meta-analysis. High-risk APS patients (triple positive APS) who used DOACs displayed an increased risk of recurrent thrombosis [risk ratio = 3.65, 95% confidence interval (95% CI): 1.49-8.93; I2 = 29%, P = 0.005] compared with those taking VKAs. Similar risk of recurrent thrombosis or major bleeding was noted in low-risk APS patients (single or double antibody-positive) upon administering DOACs or VKAs. The utilization of Rivaroxaban was associated with a high risk of recurrent thromboses (RR = 2.63; 95% CI: 1.56-4.42; I2 = 0, P = 0.0003), particularly recurrent arterial thromboses (RR = 4.52; 95% CI: 1.99-10.29; I2 = 0, P = 0.18) in overall APS patients. Comparisons of the rate of recurrent thrombosis events and major bleeding events when using dabigatran or apixaban versus VKAs yielded no statistical differences. In the absence of contraindications, this meta-analysis suggests that VKAs remain the first-choice treatment for high-risk APS patients, with DOACs a more appropriate option for low-risk APS patients. Different DOACs may exhibit different levels of efficacy and safety for thromboprophylaxis in APS patients and require further exploration.
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http://dx.doi.org/10.1097/MBC.0000000000001153DOI Listing
July 2022

Epstein-Barr virus DNA change level combined with tumor volume reduction ratio after inductive chemotherapy as a better prognostic predictor in locally advanced nasopharyngeal carcinoma.

Cancer Med 2022 Jul 19. Epub 2022 Jul 19.

Department of Head and Neck Oncology, Cancer Center, West China Hospital of Sichuan University, Chengdu, Sichuan, P.R. China.

Background: To explore the prognosis predicting ability of the combined factors, Epstein-Barr virus DNA change level (EBVCL) and tumor volume reduction ratio (TVRR) after inductive chemotherapy (IC), in locally advanced nasopharyngeal carcinoma (LANPC).

Methods: From 2010 to 2018, 299 LANPC patients were included in this retrospective study. Receiver operating characteristic (ROC) curve analysis was performed to acquire the best critical values. According to the best critical values of EBVCL and TVRR, patients were stratified into low- and high-risk groups. Kaplan-Meier and ROC curve analyses were utilized to verify the prognostic ability of the new predictor (EBVCL+TVRR). The prognostic values among EBVCL+TVRR, EBVCL, TVRR, TNM stage, and the RECIST 1.1 criteria were compared by ROC curve. The primary end points were overall survival (OS), progression-free survival (PFS), distant metastasis-free survival (DMFS), and locoregional failure-free survival (LRFFS).

Results: ROC curve analyses of TVRR on three-year survival showed the best critical values of TVRR was 32.72% for OS, 30.21% for PFS and LRFFS, 29.87% for DMFS. The best critical value of EBVCL was 127 copies/ml for OS, and 87.7 copies/ml for PFS, DMFS, and LRFFS. The three-year OS, PFS, DMFS, and LRFFS for low- and high-risk groups were 97.7% versus 78.3% (hazard ratio [HR] = 0.2398; 95% confidence interval [CI]: 0.1277-0.4502; p < 0.0001), 91.1% versus 60.9% (HR = 0.3294; 95% CI: 0.2050-0.5292; p < 0.0001), 94.2% versus 68.7% (HR = 0.2413; 95% CI: 0.1284-0.4535; p < 0.0001) and 97.8% versus 77.9% (HR = 0.3078; 95% CI: 0.1700-0.5573; p = 0.0001), respectively. The maximal area under ROC curve of EBVCL+TVRR, EBVCL, TVRR, TNM stage, and RECIST 1.1 criteria for three-year OS was 0.829, 0.750, 0.711, 0.555, and 0.605, respectively.

Conclusion: The new-developed indicator (EBVCL+TVRR) could better predict the LANPC patient's survival after IC compared with TNM stage system or RECIST 1.1 criteria.
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http://dx.doi.org/10.1002/cam4.4964DOI Listing
July 2022

Predicting Histological Healing and Recurrence in Ulcerative Colitis by Assessing Mucosal Vascular Pattern Under Narrow-Band Imaging Endoscopy.

Front Med (Lausanne) 2022 30;9:869981. Epub 2022 Jun 30.

Department of Gastroenterology, The First Affiliated Hospital of Weifang Medical College, Weifang People's Hospital, Weifang, China.

This study investigated the predictive value of narrow-band imaging (NBI) endoscopic staging of different mucosal vascular patterns (MVPs) in patients with ulcerative colitis (UC) for histological healing or clinical recurrence of patients with UC. A total of 124 patients with UC in clinical remission attending the First Affiliated Hospital of Weifang Medical College were included in the study and underwent NBI colonoscopy. Inflammatory activity was assessed in the intestine using the Mayo endoscopic score (MES) and the MVP. Mucosal inflammation was histologically graded using the Nancy index (NI). The colons of 124 patients with UC were staged according to NBI endoscopic MVP staging criteria. The differences between NBI colonoscopy MVP typing and white light endoscopic MES in assessing histological healing (HH) were statistically significant ( < 0.001), and there was a moderate correlation between MES and the degree of HH ( = 0.471, < 0.001). In addition, there was a significant correlation between the severity of mucosal activity determined by white light endoscopy (WLE) and MVP staging ( = 0.811, < 0.001). The differences between NBI endoscopic MVP staging and white light endoscopic MES in assessing UC recurrence were statistically significant ( < 0.001). Spearman's correlation analysis showed a moderate correlation between NBI endoscopic MVP staging and clinical recurrence. NBI endoscopic MVP staging can predict HH and clinical recurrence status better than WLE.
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http://dx.doi.org/10.3389/fmed.2022.869981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284416PMC
June 2022

A Retrospective Comparative Study of Modified Percutaneous Endoscopic Transforaminal Discectomy and Open Lumbar Discectomy for Gluteal Pain Caused by Lumbar Disc Herniation.

Front Surg 2022 22;9:930036. Epub 2022 Jun 22.

Department of Orthopedics, The Second Hospital of Jilin University, Changchun, China.

Introduction: This study aimed to demonstrate the safety and effectiveness of modified percutaneous endoscopic transforaminal discectomy (PETD) in the surgical management of single-segment lumbar disc herniation (LDH) gluteal pain and to determine whether it provides a better clinical outcome than open lumbar discectomy (OD).

Methods: A retrospective analysis of patients treated with modified PETD and OD for gluteal pain in LDH from January 2015 to December 2020 was conducted. Sample size was determined using a priori power analysis. Demographic information, surgical outcomes including procedure time (minutes), intraoperative blood loss (mL), hospital days, costs (RMB), fluoroscopy shots, recurrence and complications, etc., were recorded and analyzed. Prognostic outcomes were assessed using the visual analog scale (VAS), the Oswestry Disability Index (ODI), the Japanese Orthopedic Association Score (JOA) and modified MacNab criteria. The preoperative and postoperative VAS, ODI and JOA scores were recorded by two assistants. When the results were inconsistent, the scores were recorded again by the lead professor until all scores were consistently recorded in the data. MRI was used to assess radiological improvement and all patients received follow-ups for at least one year.

Results: The sample size required for the study was calculated by a priori analysis, and a total of 72 participants were required for the study to achieve 95% statistical test power. A total of 93 patients were included, 47 of whom underwent modified PETD, and 46 of whom underwent OD. In the modified PETD intragroup comparison, VAS scores ranged from 7.14 ± 0.89 preoperatively to 2.00 ± 0.58, 2.68 ± 0.70, 2.55 ± 0.69, 2.23 ± 0.81, and 1.85 ± 0.72 at 7 days, 1 month, 3 months, 6 months, and 12 months postoperatively. Patients showed significant pain relief postoperatively ( < 0.01). According to the modified MacNab score, the excellent rate in the PETD group was 89.36%. There was no significant difference compared to the OD group (89.13%,  > 0.05). Complication rates were lower ( > 0.05) but recurrence rates were higher ( > 0.05) in the modified PETD group than in the OD group. The modified PETD group had a faster operative time ( < 0.01), shorter hospital stay ( < 0.01), less intraoperative bleeding ( < 0.01), and less financial burden to the patient ( < 0.01) than the OD group. At 7 days postoperatively, the VAS score for low back pain was higher in the OD group than in the modified PETD group ( < 0.01). The VAS and JOA scores at 1, 3, 6, and 12 months postoperatively were not significantly different between the modified PETD and OD groups ( > 0.05), and the ODI was significantly different at 3 months postoperatively ( < 0.05).

Conclusion: Modified PETD treatment is safe and effective for gluteal pain due to L4/5 disc herniation and has the advantages of a lower complication rate, faster postoperative recovery, shorter length of stay, fewer anesthesia risks and lower cost of the procedure compared with OD. However, modified PETD has a higher recurrence rate.
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http://dx.doi.org/10.3389/fsurg.2022.930036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257256PMC
June 2022

A Geometric Approach for Real-Time Forward Kinematics of the General Stewart Platform.

Sensors (Basel) 2022 Jun 26;22(13). Epub 2022 Jun 26.

School of Intelligent Systems Engineering, Sun Yat-sen University, Guangzhou 510275, China.

This paper presents a geometric approach for real-time forward kinematics of the general Stewart platform, which consists of two rigid bodies connected by six general serial manipulators. By describing the rigid-body motion as exponential of twist, and taking advantage of the product of exponentials formula, a step-by-step derivation of the proposed algorithm is presented. As the algorithm naturally solves all passive joint displacements, the correctness is then verified by comparing the forward-kinematic solutions from all chains. The convergence ability and robustness of the proposed algorithm are demonstrated with large amounts of numerical simulations. In all test cases, the proposed algorithm terminates within four iterations, converging with near-quadratic speed. Finally, the proposed algorithm is also implemented on a mainstream embedded motion controller. Compared with the incremental method, the proposed algorithm is more robust, with an average execution time of 0.48 ms, meeting the requirements of most applications, such as kinematic calibration, motion simulation, and real-time control.
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http://dx.doi.org/10.3390/s22134829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269243PMC
June 2022

Effects of Recombinant Human Epidermal Growth Factor Eye Drops Combined with Phacoemulsification on Short- and Long-Term Visual Acuity Recovery and Related Dry Eye Complications in Patients with Senile Cataract.

Comput Math Methods Med 2022 28;2022:1041558. Epub 2022 Jun 28.

Glaucoma and Cataract Department, Enshi Huiyi Ophthalmic Hospital, Enshi, Hubei 445000, China.

Objective: To investigate the effects of recombinant human epidermal growth factor eye drops combined with phacoemulsification on short- and long-term visual acuity recovery and related dry eye complications in patients with senile cataract.

Methods: Sixty patients with senile cataract cured from January 2019 to January 2021 were enrolled in our hospital. The patients in the control group were arbitrarily assigned into the control and the research group. The former group received phacoemulsification, and the latter group received recombinant human epidermal growth factor (RhEGF) eye drops combined with phacoemulsification. The curative effect, the incidence of xerophthalmia, short-term and long-term vision improvement, changes of corneal endothelial cells, serum factors, and life quality scores were compared.

Results: The effective rate of the research group was 90.00%, and the effective rate of the control group was 66.67%; the curative effect of the research group was higher than that of the control group ( < 0.05). The incidence of dry eye in the research group was lower than that in the control group ( < 0.05). The short-term and long-term visual acuity improvement effect of the research group was better than that of the control group ( < 0.05). After treatment, the density of corneal endothelial cells in the research group was higher than that in the control group, while the proportion of hexagonal cells and the coefficient of variation of corneal endothelial cells in the research group were lower than those in the control group ( < 0.05). After treatment, IL-6 and TNF- in the research group were lower than those in the control group ( < 0.05). Compared with the control group, the physical function, psychological function, social function, and healthy self-cognition scores of the research group were all lower ( < 0.05).

Conclusion: Cataract is the leading cause of blindness in the world. With the continuous improvement of cataract phacoemulsification technology, the incidence of some serious complications has gradually lessened. Xerophthalmia is one of the most obvious and predictable complications after cataract surgery and may affect the recovery of postoperative visual acuity. Recombinant human epidermal growth factor eye drops can effectively enhance the visual acuity of patients, promote the curative effect, and strengthen the life quality.
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http://dx.doi.org/10.1155/2022/1041558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256321PMC
July 2022

ZnV(PO): A New Rocking-Chair-Type Cathode Material with High Specific Capacity Derived from Zn/H Cointercalation for Aqueous Zn-Ion Batteries.

ACS Appl Mater Interfaces 2022 Jul 6;14(28):32066-32074. Epub 2022 Jul 6.

College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China.

Phosphate cathode materials with a stable and open framework structure are expected to be one of the favorable cathode materials for aqueous zinc-ion batteries (AZIBs). However, the slow migration rate of Zn and complex mechanism in aqueous electrolyte are serious problems that limit their application at the present. Here, a new rocking-chair-type cathode material ZnV(PO)@C ([email protected]) for AZIBs is synthesized for the first time and evaluated using a composite carbon coating to improve the electronic conductivity. Benefiting from the two-electron reaction of vanadium and the cointercalation of Zn/H, [email protected]/30%BP delivers a specific capacity as high as 120 mAh·g at 0.04 A·g. A good capacity retention of 80% after 400 cycles at 1 A·g is also obtained, which is attributed to the stable crystal structure and the cointercalation reaction of Zn/H. The reaction mechanism is investigated by X-ray diffraction (XRD), XRD, X-ray photoelectron spectroscopy (XPS), and energy dispersive spectroscopy (EDS). This work not only provides a new phosphate cathode material for AZIBs but also gives a new strategy for improving the specific capacity of phosphate cathode material.
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http://dx.doi.org/10.1021/acsami.2c07525DOI Listing
July 2022

Inhibition of Heat Shock Protein B8 Alleviates Retinal Dysfunction and Ganglion Cells Loss Via Autophagy Suppression in Mouse Axonal Damage.

Invest Ophthalmol Vis Sci 2022 06;63(6):28

Eye Center, RenMin Hospital of Wuhan University, Wuhan, Hubei Province, People's Republic of China.

Purpose: Heat shock protein B8 (HspB8) can be upregulated rapidly in many pathologic processes, but its role in traumatic optic neuropathy remains unclear. In this study, we investigated the involvement of autophagy in the effects of HspB8 by using the optic nerve crush (ONC) model.

Methods: Male C57BL/6J mice were intravitreally injected with recombinant adeno-associated virus type 2 (AAV2-shHspB8 or AAV2-GFP) and subsequently received ONC by a self-closing tweezers. Western blot and immunohistochemistry staining were used to evaluate the expression of HspB8. We conducted retinal flat-mount immunofluorescence to measure the quantities of retinal ganglion cells (RGCs), and full-field flash electroretinogram (ff-ERG) and optomotor response (OMR) were used to evaluate retinal function. The autophagy level was reflected by western blot, immunohistochemistry staining, and transmission electron microscope (TEM) images. We also applied 3-methyladenine (3MA) and rapamycin (Rapa) to regulate autophagy level in optic nerve injury.

Results: ONC stimulated the expression of HspB8. Declines of RGCs and ff-ERG b-wave amplitudes resulting from ONC can be alleviated by HspB8 downregulation. Increased autophagy activity after ONC was observed; however, this change can be reversed by intravitreal injection of AAV2-shHspB8. Furthermore, application of autophagy inhibitor 3MA had the same neuroprotective effects as AAV2-shHspB8, as illustrated by ff-ERG and quantities of RGCs. Also, protection of AAV2-shHspB8 was compromised by the autophagy activator Rapa.

Conclusions: Inhibition of HspB8 in mice optic nerve injury had neuroprotective effects, which may be derived from its downregulation of autophagy.
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http://dx.doi.org/10.1167/iovs.63.6.28DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248752PMC
June 2022

Leakage Current Sensor and Neural Network for  MOA Monitoring.

Comput Intell Neurosci 2022 17;2022:6728900. Epub 2022 Jun 17.

Ma'anshan Electric Power Supply Company, State Grid Anhui Electric Power Co Ltd, Ma'anshan 243000, China.

Metal-oxide arrester (MOA) has been widely used in electric power systems. The leakage current monitoring of MOA can not only detect the MOA's running state continuously and intelligently but also reduce the unexpected outage of the equipment, which is also beneficial to the stability of the grid. The MOA loses its protection function due to various faults caused by excessive leakage current in actual running. This article studies the monitoring method of MOA based on leakage current sensor and back propagation (BP) neural network. At first, we design a novel leakage current sensor to acquire the leakage current of MOA. Then, the leakage current measurement of MOA based on harmonic analysis is proposed. Finally, the strong training ability of the BP neural network is used to train some key parameters that can reflect the aging of MOA so as to monitor the MOA state. The experimental results show that the leakage current acquired from the simulation is close to the actual leakage current that needs to be measured. It is also shown that the proposed method has good anti-interference and can effectively monitor the aging of MOA. Through the training of the BP neural network, the experiments prove that the training method in this article is superior to other neural network training methods obviously.
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http://dx.doi.org/10.1155/2022/6728900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232350PMC
June 2022

Quantifying the spatial pattern of urban heat islands and the associated cooling effect of blue-green landscapes using multisource remote sensing data.

Sci Total Environ 2022 Jun 21;843:156829. Epub 2022 Jun 21.

College of Mathematics and Computer Science, Zhejiang A&F University, Hangzhou 311300, China; Key Laboratory of Forestry Intelligent Monitoring and Information Technology of Zhejiang Province, Hangzhou 311300, China; Key Laboratory of State Forestry and Grassland Administration on Forestry Sensing Technology and Intelligent Equipment, Hangzhou 311300, China. Electronic address:

Surface urban heat islands (SUHIs) are a global concern. Although their spatial pattern and the cooling effect of blue-green landscapes have been documented, exploring more accurate and quantitative results is still necessary. For Hangzhou, we combined nighttime light (NTL) data with LST images to investigate the spatial morphology of SUHIs and analyze the cooling effect of blue-green landscapes. The radiative transfer equation (RTE) method was used to derive the land surface temperature (LST). Then, based on the unique feature of Luojia1-01 NTL data, the concentric zone model (CZM) was proposed to depict the urban spatial structure. The CZM was applied to construct a number of equal-area concentric belts along the urban-rural gradient to determine the SUHI range and the corresponding blue-green landscape cooling effects. Finally, local Moran's I indices were adopted to identify the cold-hot spots of the SUHI and the relationship with land use. The minimum, average and maximum LSTs were 21.81 °C, 32.79 °C and 44.79 °C, respectively. Additionally, 59.16 % of the study area was affected by the SUHI, and the mean LST inside the SUHI was 36.4 °C, clearly higher than that of the rural area. The SUHI hotpots were clustered in regions with intensive human activities, forming archipelagos. Due to the different blue-green landscape densities, the cooling capacity had spatial heterogeneity in different urban rural belts (URBs), and the cooling capacity of URB was approximately 71 times that of URB. The cooling efficiency increased with blue-green landscape density in general; hence, blue-green landscape density thresholds of 40 % and 70 % were recommended in the urban planning of different urban function zones. Relating the pattern of NTL data to LST images provide meaningful insight into the spatial pattern of SUHIs and the optimization of urban planning.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156829DOI Listing
June 2022

Dynamic Measurements of Cerebral Blood Flow Responses to Cortical Spreading Depolarization in the Murine Endovascular Perforation Subarachnoid Hemorrhage Model.

Transl Stroke Res 2022 Jun 24. Epub 2022 Jun 24.

Department of Neurosurgery, the First Affiliated Hospital of Chongqing Medical University, 1 Youyi Rd, Chongqing, 400016, People's Republic of China.

Delayed cerebral ischemia (DCI) is the most severe complication after subarachnoid hemorrhage (SAH), and cortical spreading depolarization (CSD) is believed to play a vital role in it. However, the dynamic changes in cerebral blood flow (CBF) in response to CSD in typical SAH models have not been well investigated. Here, SAH was established in mice with endovascular perforation. Subsequently, the spontaneous CBF dropped instantly and then returned to baseline rapidly. After KCl application to the cortex, subsequent hypoperfusion waves occurred across the groups, while a lower average perfusion level was found in the SAH groups (days 1-7). Moreover, in the SAH groups, the number of CSD decreased within day 7, and the duration and spreading velocity of the CSD increased within day 3 and day 14, respectively. Next, we continuously monitored the local field potential (LFP) in the prefrontal cortex. The results showed that the decrease in the percentage of gamma oscillations lasted throughout the whole process in the SAH group. In the chronic phase after SAH, we found that the mice still had cognitive deficits but experienced no obvious tissue damage. In summary, SAH negatively affects the CBF responses to CSD and the spontaneous LFP activity and causes long-term cognitive deficits in mice. Based on these findings, in the specific phase after SAH, DCI is induced or exacerbated more easily by potential causers of CSD in clinical practice (edema, erythrocytolysis, inflammation), which may lead to neurological deterioration.
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http://dx.doi.org/10.1007/s12975-022-01052-1DOI Listing
June 2022

A Data-Driven Optimized Mechanism for Improving Online Collaborative Learning: Taking Cognitive Load into Account.

Int J Environ Res Public Health 2022 06 7;19(12). Epub 2022 Jun 7.

Key Laboratory of Intelligent Education Technology and Application of Zhejiang Province, Zhejiang Normal University, Jinhua 321004, China.

Research on online collaborative learning has explored various methods of collaborative improvement. Recently, learning analytics have been increasingly adopted for ascertaining learners' states and promoting collaborative performance. However, little effort has been made to investigate the transformation of collaborative states or to consider cognitive load as an essential factor for collaborative intervention. By bridging collaborative cognitive load theory and system dynamics modeling methods, this paper revealed the transformation of online learners' collaborative states through data analysis, and then proposed an optimized mechanism to ameliorate online collaboration. A quasi-experiment was conducted with 91 college students to examine the potential of the optimized mechanism in collaborative state transformation, awareness of collaboration, learning achievement, and cognitive load. The promising results demonstrated that students learning with the optimized mechanism performed significantly differently in collaboration and knowledge acquisition, and no additional burden in cognitive load was noted.
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http://dx.doi.org/10.3390/ijerph19126984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222686PMC
June 2022

Meta-Analysis of Rumen-Protected Methionine in Milk Production and Composition of Dairy Cows.

Animals (Basel) 2022 Jun 9;12(12). Epub 2022 Jun 9.

Department of Animal Science, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163316, China.

This study aims to evaluate the influence of rumen-protected methionine (RPM) on the milk yield and milk compositions of dairy cows by employing a meta-analysis method. The articles in the publication databases between January 2010 and January 2022 which reported on various concentrations of RPM supplements in dairy cow diets and then monitored the milk yield and milk compositions were searched. A total of 14 studies were included, covering 27 treatments with a total of 623 dairy cows. Comprehensive Meta-Analysis V3 was used for statistical analysis, the forest map was drawn by the standard mean difference (SMD) with a 95% confidence interval (95% CI), and the SMD was calculated by a random effect model. The dose effect curve was drawn by fitting the SMD and RPM dose of each study to explore the optimal dosage of RPM. Compared with the basal diet, the RPM supplement significantly increased the percentages of milk fat (SMD (95% CI): 1.017% [0.388, 1.646]) and milk protein (SMD (95% CI): 0.884 [0.392, 1.377]). However, the milk yield (SMD (95% CI): 0.227 kg/d [-0.193, 0.647]) and lactose concentration (SMD (95% CI): 0.240% [-0.540, 1.020]) were not affected. The subgroup analysis found that the effect of the RPM supplement on the milk fat and milk protein was greater in the high-protein feed than in the low-protein feed. Multiple regression analysis showed that feeding RPM significantly improved the milk yield and milk protein percentage of dairy cows. The results of the dose-effect analysis show that the optimal range for the RPM was 7.5-12.5 g/d. RPM supplements in a dairy diet can improve the milk protein percentages and milk fat percentages of dairy cows.
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http://dx.doi.org/10.3390/ani12121505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219501PMC
June 2022

The safety profile of a protocolized transbronchial cryobiopsy program utilizing a 2.4 mm cryoprobe for interstitial lung disease.

Respir Med 2022 Jun 6;200:106913. Epub 2022 Jun 6.

Department of Medicine, USA; Division of Pulmonary, Critical Care Medicine, Allergy, and Clinical Immunology, USA.

Introduction: Transbronchial lung cryobiopsy (TBLC) has emerged as a promising alternative to surgical lung biopsy for the diagnosis of interstitial lung disease. However, uncertainty remains regarding its overall complications due to a lack of procedural standardization including the size of cryoprobe utilized.

Methods: This is a prospective cohort study of a protocolized transbronchial cryobiopsy program utilizing a 2.4 mm cryoprobe. 201 consecutive subjects were enrolled at a single academic center.

Results: The average biopsy size was 106.2 ± 39.3 mm. Complications included a total pneumothorax rate of 4.9% with 3.5% undergoing chest tube placement. Severe bleeding defined by the Nashville Working Group occurred in 0.5% of cases. There were no deaths at 30-days.

Discussion: A protocolized transbronchial cryobiopsy program utilizing a 2.4 mm cryoprobe in can achieve a high diagnostic yield with a favorable safety profile.
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http://dx.doi.org/10.1016/j.rmed.2022.106913DOI Listing
June 2022

Discovering Interdisciplinary Research Based on Neural Networks.

Front Bioeng Biotechnol 2022 3;10:908733. Epub 2022 Jun 3.

Department of Information Security, Naval University of Engineering, Wuhan, China.

Interdisciplinary research promotes the emergence of scientific innovation. Researchers want to find interdisciplinary research in their research field. However, the number of scientific papers published today is increasing, and completing this task by hand is time-consuming and laborious. A neural network is a machine learning model that simulates the connection mode of neurons in the human brain. It is an important application of bionics in the artificial intelligence field. This paper proposes an approach to discovering interdisciplinary research automatically. The method generates an IRD-BERT neural network model for discovering interdisciplinary research based on the pre-trained model BERT. IRD-BERT is used to simulate the domain knowledge of experts, and author keywords can be projected into vector space by this model. According to the keyword distribution in the vector space, keywords with semantic anomalies can be identified. Papers that use these author keywords are likely to be interdisciplinary research. This method is applied to discover interdisciplinary research in the deep learning research field, and its performance is better than that of similar methods.
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http://dx.doi.org/10.3389/fbioe.2022.908733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203848PMC
June 2022

Estimation of the fraction of soil-borne particulates in indoor air by PMF and its impact on health risk assessment of soil contamination in Guangzhou, China.

Environ Pollut 2022 Sep 14;308:119623. Epub 2022 Jun 14.

Guangdong Provincial Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China. Electronic address:

The fraction of soil-borne particulates in indoor air (fspi), a principal exposure factor in health risk assessment of soil, is used to calculate the inhaled dose of contaminants in air particulates (PM) from soil. To investigate the fspi, consecutive 24-h PM samples (n = 180) of indoor ambient were collected from September 2019 to January 2020 in Guangzhou main urban areas, China. The concentrations of twenty-six metal elements, five anions, organic carbon (OC) and elemental carbon (EC) in samples were measured. The sources of indoor ambient PM and the value of fspi were identified by the method of Positive Matrix Factor analysis (PMF). Results showed that the main sources contributing to indoor PM content were combustion sources (50.53%) and vehicular sources (28.17%). The soil sources (the local fspi) were 19.96%. The soil contents of indoor PM in Guangzhou main urban areas were in accordance with those in similar monsoon climate regions, such as Malaysia. The health risks of the inhalation route were dropped by about 62% for some common brownfield contaminants (Cr (VI), Ni, Be and Cd) with the investigated local fspi in Guangzhou main urban areas, compared with using the fspi (0.8) recommended by the C-RAG model in China. The results supplied a new effective methodology for estimation of the local fspi value in health risk assessment of soil contamination in urban areas.
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http://dx.doi.org/10.1016/j.envpol.2022.119623DOI Listing
September 2022

Correction to: Homologous targeting nanoparticles for enhanced PDT against osteosarcoma HOS cells and the related molecular mechanisms.

J Nanobiotechnology 2022 Jun 16;20(1):286. Epub 2022 Jun 16.

Department of Orthopedic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.

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http://dx.doi.org/10.1186/s12951-022-01492-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205071PMC
June 2022

YAP knockdown in combination with ferroptosis induction increases the sensitivity of HOS human osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy.

Photodiagnosis Photodyn Ther 2022 Jun 12;39:102964. Epub 2022 Jun 12.

Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing 400016, China; Orthopedic Laboratory of Chongqing Medical University, Yuzhong, Chongqing 400016, China. Electronic address:

Background And Aims: This study was designed to explore the effects of Yes-associated protein (YAP) knockdown on human osteosarcoma (HOS) cell sensitivity to Pyropheophorbide-α methyl ester-mediated photodynamic therapy (MPPa-PDT), and to assess how YAP silencing in combination with treatment with the ferroptosis inducer Erastin improves HOS cell sensitivity to MPPa-PDT in an effort to better clarify the molecular mechanisms underlying these phenotypes.

Methods: At 12 h post-MPPa-PDT, Hoechst staining and flow cytometry were conducted to evaluate the apoptotic death of HOS cells. The expression of YAP in these cells at 12 h post-MPPa-PDT treatment was assessed via Western blotting and immunofluorescent staining. BODIPY-C11 was used to evaluate lipid peroxidation. Following shYAP lentiviral transduction, Western blotting was conducted to assess the expression of proteins associated with proliferation, apoptosis, and ferroptosis. EdU assays and clonogenic assays were performed to analyze cellular proliferation. Erastin-treated HOS cells were used to establish a ferroptosis model. Western blotting was used to measure ferroptosis-associated protein levels following shYAP and erastin treatment, while changes in proliferation and MDA levels in each group were examined using an MDA kit.

Results: At 12 h post-MPPa-PDT, HOS cells exhibited apoptotic characteristics including nuclear fragmentation and pyknosis, with concomitant increases in apoptosis-associated proteins as detected via Western blotting and apoptotic induction as measured via flow cytometry. Phosphorylated YAP levels fell and non-phosphorylated YAP levels rose following such treatment. Transfection with shYAP was successful as a means of generating stable HOS cell lines, and Western blotting analyses of these cells revealed reductions in proteins associated with cellular proliferation together with the upregulation of apoptosis-related proteins.  MDA assays indicated that erastin combined with YAP knockdown enhanced the sensitivity of HOS cells to MPPa-PDT treatment.

Conclusions: These data indicate that ferroptosis and YAP knockdown can enhance osteosarcoma cell sensitivity to MPPa-PDT therapy.
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http://dx.doi.org/10.1016/j.pdpdt.2022.102964DOI Listing
June 2022

Impact of Early Rehabilitation on Outcomes in Patients With Acute Ischemic Stroke After Endovascular Treatment.

Front Neurol 2022 23;13:877773. Epub 2022 May 23.

Department of Pain and Rehabilitation, Xinqiao Hospital, Army Medical University, Chongqing, China.

Background: This study aims to examine the effects of early rehabilitation on functional outcomes in patients with acute ischemic stroke treated with endovascular treatment (EVT).

Methods: Eligible patients with large vessel occlusion stroke treated with EVT, who received early rehabilitation or standard care treatment during hospitalization, were enrolled in a multicenter registration, prospective observational study, a registration study for Critical Care of Acute Ischemic Stroke After Recanalization. Early rehabilitation was defined as rehabilitation interventions initiated within 1 week after acute stroke. The primary outcome was the favorable functional outcome (defined as modified Rankin Scale scores of 0 to 2) at 90 days. Independent association between early rehabilitation and the primary outcome was investigated using multivariable logistic regression in the entire sample and in subgroups.

Results: A total of 1,126 patients (enrolled from July 2018 to May 2019) were included in the analyses, 273 (24.2%) in the early rehabilitation group and 853 (75.8%) in the standard care group. There was no significant difference in favorable functional outcomes at 90 days between the two groups (45.4 vs. 42.6%, = 0.41). Patients in the early rehabilitation group had a lower death rate within 90 days compared with the standard care group (6.2 vs. 20.5%, < 0.01). The multivariable logistic regression analyses showed that the early rehabilitation was not significantly associated with the favorable functional outcome at 90 days (adjusted odds ratio, 1.01 [95% CI, 0.70-1.47]; = 0.95). There was no significant difference between subgroups in the favorable functional outcome at 90 days. No significant interaction was found between subgroups.

Conclusions: Patients with stroke receiving early rehabilitation had a lower death rate. However, these clinically meaningful effects of early rehabilitation did not show on functional outcome at 90 days in patients with large vessel occlusion stroke treated with EVT.

Registration: URL: http://www.chictr.org.cn; Unique identifier: ChiCTR1900022154.
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http://dx.doi.org/10.3389/fneur.2022.877773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168462PMC
May 2022

CircSCAF8 promotes growth and metastasis of prostate cancer through the circSCAF8-miR-140-3p/miR-335-LIF pathway.

Cell Death Dis 2022 Jun 2;13(6):517. Epub 2022 Jun 2.

Department of Urology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510630, China.

Circular RNAs (circRNAs) have been increasingly linked to cancer progression. However, the detailed biological functions of circRNAs in prostate cancer (PCa) remain unclear. Using high-throughput circRNA sequencing, we previously identified 18 urine extracellular vesicle circRNAs that were increased in patients with PCa compared with those with benign prostatic hyperplasia. Spearman correlation analysis of the expression levels of the 18 circRNAs between the tumor tissue and matched urine extracellular vesicles in 30 PCa patients showed that circSCAF8 had the highest R (R = 0.635, P < 0.001). The Cox proportional hazards regression model was used to estimate the effect of circSCAF8 on progression-free survival. The in vitro and in vivo functional experiments were implemented to investigate the effects of circSCAF8 on the phenotype of PCa. We found that the knockdown of circSCAF8 in PCa cells suppressed the proliferation, migration, and invasion ability, while overexpression of circSCAF8 had the opposite effects. Similar results were observed in vivo. In a cohort of 85 patients who had undergone radical prostatectomy, circSCAF8 expression in PCa tissues was a powerful predictor of progression-free survival (HR = 2.14, P = 0.022). Mechanistically, circSCAF8 can function by binding to both miR-140-3p and miR-335 to regulate LIF expression and activate the LIF-STAT3 pathway that leads to the growth and metastasis of PCa. Collectively, our findings demonstrate that circSCAF8 contributes to PCa progression through the circSCAF8-miR-140-3p/miR-335-LIF pathway.
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http://dx.doi.org/10.1038/s41419-022-04913-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163066PMC
June 2022
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