Publications by authors named "Tao Gao"

282 Publications

A novel polymer micelle as a targeted drug delivery system for 10-hydroxycamptothecin with high drug-loading properties and anti-tumor efficacy.

Biophys Chem 2021 Sep 17;279:106679. Epub 2021 Sep 17.

Heilongjiang University of Chinese Medicine, China. Electronic address:

A novel polyethylene glycol-polycaprolactone-poly-l-tyrosine (MPEG-PCL-PTyr) amphiphilic triblock copolymer micelle was synthesized for the first time. 10-hydroxycamptothecin (HCPT) was embedded in MPEG-PCL-PTyr nanomicelles using the emulsion solvent evaporation method. A series of was conducted to confirm the structure of the compound and to evaluate the physical properties of the MPEG-PCL-PTyr nanomicelles. Cellular uptake, cytotoxicity, and apoptosis were assessed using flow cytometry and MTT assays. Confocal microscopy and flow cytometry results demonstrated that the nanocapsules carrying HCPT had significantly increased anti-tumor activity against HepG2 cells and could target HepG2 cell lysosomes with obvious liver targeting. In addition, the drug-loaded nanomicelles could significantly block the S phase of cancer cells and induce apoptosis; thus, they could be potential carriers for future 10-HCPT delivery and cancer treatment.
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http://dx.doi.org/10.1016/j.bpc.2021.106679DOI Listing
September 2021

Impact of paternal education on epigenetic ageing in adolescence and mid-adulthood: a multi-cohort study in the USA and Mexico.

Int J Epidemiol 2021 Sep 17. Epub 2021 Sep 17.

Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.

Background: Both parental and neighbourhood socio-economic status (SES) are linked to poorer health independently of personal SES measures, but the biological mechanisms are unclear. Our objective was to examine these influences via epigenetic age acceleration (EAA)-the discrepancy between chronological and epigenetic ages.

Methods: We examined three USA-based [Coronary Artery Risk Disease in Adults (CARDIA) study, Fragile Families and Child Wellbeing Study (FFCWS) and Programming Research in Obesity, Growth, Environment and Social Stressors (PROGRESS)] and one Mexico-based (Project Viva) cohort. DNA methylation was measured using Illumina arrays, personal/parental SES by questionnaire and neighbourhood disadvantage from geocoded address. In CARDIA, we examined the most strongly associated personal, parental and neighbourhood SES measures with EAA (Hannum's method) at study years 15 and 20 separately and combined using a generalized estimating equation (GEE) and compared with other EAA measures (Horvath's EAA, PhenoAge and GrimAge calculators, and DunedinPoAm).

Results: EAA was associated with paternal education in CARDIA [GEEs: βsome college = -1.01 years (-1.91, -0.11) and β
Conclusions: These findings suggest that EAA captures epigenetic impacts of paternal education independently of personal SES later in life. Longitudinal studies should explore these associations at different life stages and link them to health outcomes. EAA could be a useful biomarker of SES-associated health and provide important insight into the pathogenesis and prevention of chronic disease.
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http://dx.doi.org/10.1093/ije/dyab196DOI Listing
September 2021

Modulation of Siglec-7 Signaling Via In Situ-Created High-Affinity -Ligands.

ACS Cent Sci 2021 Aug 13;7(8):1338-1346. Epub 2021 Aug 13.

Department of Molecular Medicine, Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla 92037, California, United States.

Sialic acid-binding immunoglobulin-like lectins, also known as Siglecs, have recently been designated as glyco-immune checkpoints. Through their interactions with sialylated glycan ligands overexpressed on tumor cells, inhibitory Siglecs on innate and adaptive immune cells modulate signaling cascades to restrain anti-tumor immune responses. However, the elucidation of the mechanisms underlying these processes is just beginning. We find that when human natural killer (NK) cells attack tumor cells, glycan remodeling occurs on the target cells at the immunological synapse. This remodeling occurs through both the transfer of sialylated glycans from NK cells to target tumor cells and the accumulation of synthesized sialosides on the tumor cells. The functionalization of NK cells with a high-affinity ligand of Siglec-7 leads to multifaceted consequences in modulating a Siglec-7-regulated NK-activation. At high levels of ligand, an enzymatically added Siglec-7 ligand suppresses NK cytotoxicity through the recruitment of Siglec-7 to an immune synapse, whereas at low levels of ligand an enzymatically added Siglec-7 ligand triggers the release of Siglec-7 from the cell surface into the culture medium, preventing a Siglec-7-mediated inhibition of NK cytotoxicity. These results suggest that a glycan engineering of NK cells may provide a means to boost NK effector functions for related applications.
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http://dx.doi.org/10.1021/acscentsci.1c00064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393205PMC
August 2021

Epigenetic Age Acceleration Reflects Long-Term Cardiovascular Health.

Circ Res 2021 Aug 25. Epub 2021 Aug 25.

Preventive Medicine, Northwestern University Feinberg School of Medicine, UNITED STATES.

Epigenetic aging is a novel measure of biological age, reflecting exposures and disease risks independent of chronological age. It may serve as a useful biomarker of cardiovascular health (CVH) and/or cardiovascular disease (CVD) risk for early detection or prevention. To examine associations between GrimAge acceleration (GrimAA), a measure of epigenetic aging calculated from the residuals of GrimAge regressed on chronological age, and two repeated CVH measures: a full score for the AHA "Life's Simple 7" (diet, smoking, physical activity, BMI, blood pressure, total cholesterol, and glucose) and a clinical CVH score (BMI, blood pressure, cholesterol, and glucose). We used Illumina array DNA methylation data from two prospective cohort studies: The Coronary Artery Risk Development in Young Adults (CARDIA) study and Framingham Heart Study (FHS), to calculate GrimAA and model associations with CVH. CARDIA randomly selected 1,118 participants for assays at Y15 (2000-2001; mean age 40) and/or Y20 (2005-2006); in FHS, 2,106 Offspring participants had DNA methylation measured at exam 8 (2005-2008; mean age 66). We examined multiple cross-sectional and longitudinal models of GrimAA and each CVH score measured at CARDIA Y0-Y20 and FHS exams 7-8. In CARDIA clinical CVH score from Y0-Y20 was associated with Y15 and Y20 GrimAA (β range -0.41 to -0.21 years per 1-point increase in CVH; p range <0.01 to 0.01), as was full score (β range -0.65 to -0.67 years; p<0.01 for all). These findings were validated in FHS (clinical score β range -0.51 to -0.54 years; full score β range -0.76 to -0.83 years; p<0.01 for all). Our data demonstrate that faster GrimAA is associated with the loss of CVH from young age. Epigenetic age may be a useful biomarker of CVD risk and provides biological insight into the role of epigenetic mechanisms linking age-related CVH loss and CVD.
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http://dx.doi.org/10.1161/CIRCRESAHA.121.318965DOI Listing
August 2021

EGFL6 regulates angiogenesis and osteogenesis in distraction osteogenesis via Wnt/β-catenin signaling.

Stem Cell Res Ther 2021 07 22;12(1):415. Epub 2021 Jul 22.

Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China.

Background: Osteogenesis is tightly coupled with angiogenesis during bone repair and regeneration. However, the underlying mechanisms linking these processes remain largely undefined. The present study aimed to test the hypothesis that epidermal growth factor-like domain-containing protein 6 (EGFL6), an angiogenic factor, also functions in bone marrow mesenchymal stem cells (BMSCs), playing a key role in the interaction between osteogenesis and angiogenesis.

Methods: We evaluated how EGFL6 affects angiogenic activity of human umbilical cord vein endothelial cells (HUVECs) via proliferation, transwell migration, wound healing, and tube-formation assays. Alkaline phosphatase (ALP) and Alizarin Red S (AR-S) were used to assay the osteogenic potential of BMSCs. qRT-PCR, western blotting, and immunocytochemistry were used to evaluate angio- and osteo-specific markers and pathway-related genes and proteins. In order to determine how EGFL6 affects angiogenesis and osteogenesis in vivo, EGFL6 was injected into fracture gaps in a rat tibia distraction osteogenesis (DO) model. Radiography, histology, and histomorphometry were used to quantitatively evaluate angiogenesis and osteogenesis.

Results: EGFL6 stimulated both angiogenesis and osteogenic differentiation through Wnt/β-catenin signaling in vitro. Administration of EGFL6 in the rat DO model promoted CD31EMCN type H-positive capillary formation associated with enhanced bone formation. Type H vessels were the referred subtype involved during DO stimulated by EGFL6.

Conclusion: EGFL6 enhanced the osteogenic differentiation potential of BMSCs and accelerated bone regeneration by stimulating angiogenesis. Thus, increasing EGFL6 secretion appeared to underpin the therapeutic benefit by promoting angiogenesis-coupled bone formation. These results imply that boosting local concentrations of EGFL6 may represent a new strategy for the treatment of compromised fracture healing and bone defect restoration.
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http://dx.doi.org/10.1186/s13287-021-02487-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296592PMC
July 2021

[Clinical application of free peroneal artery perforator flap in soft tissue defect of foot and ankle].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Jun;35(6):756-760

Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, P.R.China.

Objective: To investigate clinical application of the free peroneal artery perforator flap in soft tissue defect of foot and ankle.

Methods: The clinical data of 18 patients with soft tissue defects of foot and ankle who were repaired with free peroneal artery perforator flaps between March 2019 and March 2020 were retrospectively analyzed. Among them, there were 11 males and 7 females; the age ranged from 21 to 58 years, with an average age of 45 years. The defect was located in the ankle in 2 cases, in the hindfoot in 4 cases, in the midfoot in 5 cases, and in the forefoot in 7 cases. The causes of injury included 11 cases of traffic accident, 4 cases of machine injuries, 3 cases of infection and necrosis after internal fixation. The time from injury to flap repair was 12-48 days, with an average of 24 days. The range of wound was 3 cm×3 cm to 15 cm×8 cm, and the range of skin flap was 4 cm×3 cm to 16 cm×9 cm. The flap harvesting time, operation time, intraoperative blood loss, and complications were recorded; the flap survival and patient satisfaction were observed during follow-up; and the American Orthopaedic Foot and Ankle Society (AOFAS) foot function score was used to evaluate the foot function.

Results: The flap harvesting time was 15-33 minutes (mean, 22 minutes); the operation time was 120-160 minutes (mean, 150 minutes); the intraoperative blood loss was 90-180 mL (mean, 120 mL). There were 3 cases of vascular crisis after operation, including 2 cases of arterial crisis, which survived after vascular exploration and vein graft repair; 1 case of venous crisis, partial necrosis of the skin flap, and skin grafting to cover the wound after repeated debridement. The remaining 15 skin flaps survived completely. All patients were followed up 6 months. The skin flaps were in good shape without obvious bloat. According to the AOFAS foot function score, 5 cases were excellent, 10 cases were good, and 3 cases were fair. The excellent and good rate was 83.3%.

Conclusion: The free peroneal artery perforator flap is easy to harvest, the shape and size of the flap are easy to design, and it does not damage the main blood vessels of the limb. The appearance and function of the limbs are satisfactory after operation. It can be widely used in the repair of soft tissue defects of the foot and ankle.
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http://dx.doi.org/10.7507/1002-1892.202101069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218183PMC
June 2021

Effects of cranberry beverages on oxidative stress and gut microbiota in subjects with infection: a randomized, double-blind, placebo-controlled trial.

Food Funct 2021 Aug;12(15):6878-6888

Binzhou Medical University Hospital, 661 Huanghe Second Road, Binzhou, Shandong, China.

Helicobacter pylori-induced oxidative stress plays an important role in gastric diseases. H. pylori disturbs gut microbiota. The objective is to investigate the effects of cranberry beverages on oxidative stress biomarkers and gut microbiota in H. pylori positive subjects. 171 H. pylori positive participants were randomly assigned to one of the three groups: high-dose (HCb; 480 mL cranberry beverage), low-dose (LCb; 240 mL cranberry beverage plus 240 mL placebo) and placebo (480 mL). Subjects consumed the beverages daily for 4 weeks. Fasting blood samples were analyzed for oxidative stress biomarkers. The intestinal microbiome was analyzed by 16S rRNA sequencing. Compared with the placebo, HCb resulted in a significantly higher increase of total antioxidant capacity (mean ± SD: 1.39 ± 1.69 IU mL-1vs. 0.34 ± 1.73 IU mL-1; p < 0.001) and a higher decrease of the lipid peroxidation product malondialdehyde (-7.29 ± 10.83 nmol mg-1vs. -0.84 ± 15.66 nmol mg-1; p = 0.025). A significant dose-dependent effect on the elevation of superoxide dismutase was observed (p < 0.001). Microbiome data showed that consuming HCb and LCb led to a significant reduction of Pseudomonas (p < 0.05). In conclusion, the current research showed that consuming cranberry beverages significantly improved the antioxidant status in H. pylori positive subjects, which may be related to the reshaping of gut microbiota.
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http://dx.doi.org/10.1039/d1fo00467kDOI Listing
August 2021

Addition of low-dose sufentanil to ropivacaine for reducing shivering and visceral traction pain during cesarean section.

J Int Med Res 2021 May;49(5):3000605211017000

Department of Anesthesia, Yongchuan Hospital of Chongqing Medical University, Chongqing, China.

Objective: To investigate the efficacy of low-dose sufentanil for preventing shivering and visceral traction pain during cesarean section under spinal anesthesia.

Methods: This was a prospective, randomized, controlled study. A total of 112 full-term parturients who underwent elective caesarean delivery were randomly divided into two groups. Group R received 0.75% isobaric ropivacaine intrathecally and group RS received 0.75% isobaric ropivacaine plus 5 µg sufentanil intrathecally.

Results: There were no significant differences in the maximum sensory block time, motor block time, duration of the surgery, and heart rate, mean arterial pressure, and blood oxygen saturation before and 1, 5, and 10 minutes after spinal anesthesia, and at the end of the surgery between the two groups. Shivering was significantly more common in group R (n = 30) than in group RS (n = 8). The incidence of visceral traction pain in group R (46.43%) was significantly higher than that in group RS (14.29%). There was no significant difference in the newborns' Apgar scores between the groups.

Conclusion: Adding low-dose sufentanil to ropivacaine can significantly reduce the incidence of shivering and visceral traction pain after spinal anesthesia.
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http://dx.doi.org/10.1177/03000605211017000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161908PMC
May 2021

The regulating effect of trace elements Si, Zn and Sr on mineralization of gelatin-hydroxyapatite electrospun fiber.

Colloids Surf B Biointerfaces 2021 Aug 12;204:111822. Epub 2021 May 12.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, PR China. Electronic address:

Biomineralization approaches have been increasingly adopted to synthesizing advanced materials with superior properties. Nevertheless, the potential influence of inorganic trace elements on the mineralization process of collagen has been rarely reported, despite of the significant progress achieved on exploiting the critical roles of organic polymers in regulating the collagen mineralization. To this aim, the potential roles of Si, Zn and Sr in regulating the mineralization of gelatin-hydroxyapatite (HA) composite fibers have been examined in this study. The results indicated that the incorporation of trace elements not only promoted the biomineralization of gelatin, but also led to drastic change in the mineralization behavior. In particular, the gelatin-SiHA sample showed uniform mineralization predominantly inside the fibers, with nucleation and growth directions along the c-axis of the gelatin fibers. On the contrary, the gelatin-HA sample showed nucleation outside the fibers and spherical mineral crystals on top of fibers, typical structure for heterogeneous nucleation. As the mineralization process proceeded, the gelatin-ZnHA and gelatin-SrHA samples evolved into having similar structure as the gelatin-SiHA sample, despite of showing totally different mineralization behaviors at early time. Overall, the incorporation of trace elements seemed to lower the nucleation barriers, led to a more homogeneous mineralization mode within the fiber region and formation of mineralized structures closer to those in natural bone. Moreover, mineralized samples with trace elements demonstrated improved adhesion and cytoskeleton organization of osteoblastic cells. Such finding would provide important insight for understanding the mineralization process and the optimal design of advanced biological materials.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111822DOI Listing
August 2021

Changes in Temporal and Spatial Patterns of Intrinsic Brain Activity and Functional Connectivity in Upper-Limb Amputees: An fMRI Study.

Neural Plast 2021 23;2021:8831379. Epub 2021 Apr 23.

Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.

Background: Amputation in adults is a serious procedure or traumatic outcome, one that leads to a possible "remapping" of limb representations (somatotopy) in the motor and sensory cortex. The temporal and spatial extent underlying reorganization of somatotopy is unclear. The aim of this study was to better understand how local and global structural plasticity in sensory-motor cortical networks changes temporally and spatially after upper-limb amputation.

Methods: We studied 8 healthy nonamputee control subjects and 16 complete upper-limb amputees. Resting-state MRI (rs-fMRI) was used to measure local and large-scale relative differences (compared to controls) in both the amplitude of low-frequency fluctuations (ALFF) and degree of centrality (DC) at 2 months, 6 months, and 12 months after traumatic amputation.

Results: In amputees, rs-fMRI scans revealed differences in spatial patterns of ALFF and DC among brain regions over time. Significant relative increases in ALFF and DC were detected not only in the sensory and motor cortex but also in related cortical regions believed to be involved in cognition and motor planning. We observed changes in the magnitude of ALFFs in the pre- and postcentral gyrus and primary sensory cortex, as well as in the anterior cingulate, parahippocampal gyrus, and hippocampus, 2 months after the amputation. The regional distribution of increases/decreases in ALFFs and DC documented at 2-month postamputation was very different from those at 6 and 12-month postamputation.

Conclusion: Local and wide-spread changes in ALFFs in the sensorimotor cortex and cognitive-related brain regions after upper-limb amputation may imply dysfunction not only in sensory and motor function but also in areas responsible for sensorimotor integration and motor planning. These results suggest that cortical reorganization after upper extremity deafferentation is temporally and spatially more complicated than previously appreciated, affecting DC in widespread regions.
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http://dx.doi.org/10.1155/2021/8831379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088358PMC
April 2021

Low skeletal muscle mass predicts poor clinical outcomes in patients with abdominal trauma.

Nutrition 2021 09 4;89:111229. Epub 2021 Mar 4.

Department of Intensive Care Unit, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu Province, China. Electronic address:

Objective: The aim of this study was to investigate whether low skeletal muscle mass is associated with clinical outcomes in patients with abdominal trauma.

Methods: Patients presenting to our institution with abdominal trauma from January 2010 to April 2020 were retrospectively included. Low skeletal muscle mass was defined, using computed tomography, as skeletal muscle index (SMI) at the third lumbar vertebra below the lowest sex-specific quartile within 1 wk of admission. Clinical outcomes such as complications, hospital stay, and hospital cost were recorded, and univariate and multivariate analyses were performed.

Results: Among 684 patients, 451 were eligible. Of these, 112 (24.8%) were classified as having low skeletal muscle mass, based on SMI diagnostic cutoff values (42.08 cm/m for men and 37.35 cm/m for women). Low skeletal muscle mass was significantly associated with longer hospital length of stay, longer intensive care length of stay, higher cost, higher frequency of mechanical ventilation, longer duration of vasopressor use, and higher incidence of massive transfusion and overall complications (P < 0.05). Subgroup analysis showed that pneumonia, acute gastrointestinal dysfunction, cholecystitis, digestive tract fistula, gastric fistula, and intestinal fistula were significantly associated with low skeletal muscle mass (P < 0.05). Multivariate analysis showed that low skeletal muscle mass was an independent risk factor for overall complications (odds ratio [OR], 2.44; 95% confidence interval [CI], 1.33-4.49; P = 0.004), hospital length of stay (OR, 3.49; 95% CI, 1.96-6.20; P < 0.001), and hospital cost (OR, 2.67; 95% CI, 1.48-4.80; P = 0.001).

Conclusions: Low skeletal muscle mass could be an independent predictor of poor clinical outcomes in patients with abdominal trauma.
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http://dx.doi.org/10.1016/j.nut.2021.111229DOI Listing
September 2021

[Experimental study of endothelial progenitor cells derived small extracellular vesicles for spinal cord injury repair in mice].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Apr;35(4):488-495

Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, 200233, P.R.China.

Objective: To explore the potential therapeutic effects of endothelial progenitor cells derived small extracellular vesicles (EPCs-sEVs) on spinal cord injury in mice.

Methods: EPCs were separated from femur and tibia bone marrow of 20 C57BL/6 male mice, and identified by double fluorescence staining and flow cytometry. Then the EPCs were passaged and the cell supernatants from P2-P4 generations EPCs were collected; the EPCs-sEVs were extracted by ultracentrifugation and identified by transmission electron microscopy, nanoflow cytometry, and Western blot. Forty C57BL/6 female mice were randomly divided into 4 groups ( =10). The mice were only removed T lamina in sham group, and prepared T spinal cord injury models in the model group and the low and high concentration intervention groups. After 30 minutes, 3 days, and 7 days of operation, the mice in low and high concentration intervention groups were injected with EPCs-sEVs at concentrations of 1×10 and 1×10 cells/mL through the tail vein, respectively. The behavioral examinations [Basso Mouse Scale (BMS) score, inclined plate test, Von Frey test] , and the gross, HE staining, and immunohistochemical staining were performed to observe the structural changes of the spinal cord at 4 weeks after operation. Another 3 C57BL/6 female mice were taken to prepare T spinal cord injury models, and DiR-labeled EPCs- sEVs were injected through the tail vein. After 30 minutes, imaging was used to observe whether the EPCs-sEVs reached the spinal cord injury site.

Results: After identification, EPCs and EPCs-sEVs derived from mouse bone marrow were successfully obtained. imaging of the spinal cord showed that EPCs-sEVs were recruited to the spinal cord injury site within 30 minutes after injection. There was no significant difference in BMS scores and the maximum angle of the inclined plate test between two intervention groups and the model group within 2 weeks after operation ( >0.05), while both were significantly better than the model group ( <0.05) after 2 weeks. The Von Frey test showed that the mechanical pain threshold of the two intervention groups were significantly higher than that of model group and lower than that of sham group ( <0.05); there was no significant difference between two intervention groups ( >0.05). Compared with the model group, the injured segment of the two intervention groups had smaller spinal cord tissue defects, less mononuclear cells infiltration, more obvious tissue structure recovery, and more angiogenesis, and these differences were significant ( <0.05); there was no significant difference between the two intervention groups.

Conclusion: EPCs-sEVs can promote the repair of spinal cord injury in mice and provide a new plan for the biological treatment of spinal cord injury.
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http://dx.doi.org/10.7507/1002-1892.202009130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171620PMC
April 2021

Cell-autonomous retinoic acid receptor signaling has stage-specific effects on mouse enteric nervous system.

JCI Insight 2021 May 24;6(10). Epub 2021 May 24.

Children's Hospital of Philadelphia Research Institute, Philadelphia, Pennsylvania, USA.

Retinoic acid (RA) signaling is essential for enteric nervous system (ENS) development, since vitamin A deficiency or mutations in RA signaling profoundly reduce bowel colonization by ENS precursors. These RA effects could occur because of RA activity within the ENS lineage or via RA activity in other cell types. To define cell-autonomous roles for retinoid signaling within the ENS lineage at distinct developmental time points, we activated a potent floxed dominant-negative RA receptor α (RarαDN) in the ENS using diverse CRE recombinase-expressing mouse lines. This strategy enabled us to block RA signaling at premigratory, migratory, and postmigratory stages for ENS precursors. We found that cell-autonomous loss of RA receptor (RAR) signaling dramatically affected ENS development. CRE activation of RarαDN expression at premigratory or migratory stages caused severe intestinal aganglionosis, but at later stages, RarαDN induced a broad range of phenotypes including hypoganglionosis, submucosal plexus loss, and abnormal neural differentiation. RNA sequencing highlighted distinct RA-regulated gene sets at different developmental stages. These studies show complicated context-dependent RA-mediated regulation of ENS development.
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http://dx.doi.org/10.1172/jci.insight.145854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262371PMC
May 2021

Electrochemical Trans-Channel Assay for Efficient Evaluation of Tumor Cell Invasiveness.

ACS Appl Mater Interfaces 2021 Apr 9;13(15):17268-17275. Epub 2021 Apr 9.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China.

Efficiently assessing the invasive capability of tumor cells is critical both for the research and treatment of cancer. Here, we report a novel method called the electrochemical trans-channel assay for efficient evaluation of tumor cell invasiveness. A bioinspired extracellular matrix degradation model (EDM) has been first fabricated on a porous anodic alumina (PAA) membrane to construct the electrochemical apparatus. Upon contacting the invasive tumor cells, invasive capability can be sensitively evaluated by the degree of EDM impairment, which is recorded by the electrochemical trans-channel ionic currents in a label-free manner. Compared to the most commonly used trans-well migration method, this assay can be accomplished in an efficient way that is significantly faster (20 min) and more convenient. Besides, quantitation can also be realized for monitoring the invasion process, which cannot be achieved by other currently used methods. Our proposed electrochemical trans-channel assay method has shown a synergistic effect for the evaluation of tumor cell invasiveness, providing a promising method for clinical assessment or prognostic applications of tumor metastasis.
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http://dx.doi.org/10.1021/acsami.1c01236DOI Listing
April 2021

Bandage lens for treatment of corneosclera melting two weeks after pterygium surgery: A case report.

Medicine (Baltimore) 2021 Apr;100(14):e25348

Eye Center, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, China.

Introduction: Pterygium is a common chronic ocular surface condition in ophthalmology.At present, the main treatment modality is surgical resection. Although the recurrence rate can be controlled to varying degrees, some patients can still develop serious complications, such as scleral melting, corneal melting, and even corneal perforation.We report a case of severe corneal and scleral melting after pterygium surgery treated with a bandage lens.

Patient Information: A 60-year-old male who developed corneoscleral melting after pterygium surgery.

Diagnosis: This patient was diagnosed with corneoscleral melting.

Interventions: This patient was treated with a bandage lens and eye drops.

Outcomes: He was treated with a bandage lens, and the tear break-up time (BUT) was prolonged. After 12 days the cornea and sclera were completely cured and the bandage lens was removed after one month.

Conclusion: After pterygium surgery, various factors affect the occurrence of serious complications of autolysis. Mainly on ocular parts, such as the cornea and sclera, a bandage lens can stabilize the ocular surface tear film and prolong the tear break-up time (BUT), effectively prevent corneoscleral melting and promote corneoscleral cure.
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http://dx.doi.org/10.1097/MD.0000000000025348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036106PMC
April 2021

Covalent immobilization of DJK-5 peptide on porous titanium for enhanced antibacterial effects and restrained inflammatory osteoclastogenesis.

Colloids Surf B Biointerfaces 2021 Jun 15;202:111697. Epub 2021 Mar 15.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, PR China. Electronic address:

Currently, implant-related bone infection characterized by aggravated infection-induced inflammatory responses and osteolysis, remains a severe challenge in orthopedic surgery, especially in patients with osteoporosis. Attempts to control such responses using biomaterials with combined immunomodulatory and anti-bacterial properties may provide novel strategies. Herein, DJK-5, a class of host defense peptides (HDPs) with established antimicrobial and immunomodulatory functions, was introduced into porous Ti alloy. Our results indicated that the DJK-5 immobilized surfaces showed intrinsically multifunctional properties, including antibacterial ability, anti-inflammation, biocompatibility and osteolysis-inhibiting properties. The results demonstrated that the antibacterial efficiency of DJK-5 functionalized surfaces was over 90 % for both Gram-positive and Gram-negative bacteria. Specifically, DJK-5 functionalized samples also possessed the excellent anti-bacterial activity against a mixture of bacterial strains, including S. aureus, S. epidermidis and P. aeruginosa, with an antibacterial rate against mixed bacteria reaching 91.36 %, as well as reduced biofilm formation. The remarkable anti-bacterial efficacy was likely based on the direct anti-bacterial effect of DJK-5, which destroyed the integrity of bacteria membranes, leading to the leakage of intracellular materials. Additionally, the immobilized DJK-5 surfaces could indirectly kill bacteria through promoted macrophage capacity to bacteria uptake. Furthermore, DJK-5 functionalized surfaces suppressed inflammatory reaction by decreasing the release of pro-inflammatory factors and increasing the secretions of anti-inflammatory factors, and thereby impeded the activation of NF-κB signal pathway, which resulted in the disruption of the actin rings and decreased Tracp5b expressions. Based on these promising findings, the multi-functional DJK-5 immobilized titanium represents an efficient alternative to realize better osseointegration in sever implant-associated bacterial infections.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111697DOI Listing
June 2021

Gastric Electrical Pacing Reduces Apoptosis of Interstitial Cells of Cajal via Antioxidative Stress Effect Attributing to Phenotypic Polarization of M2 Macrophages in Diabetic Rats.

Oxid Med Cell Longev 2021 28;2021:1298657. Epub 2021 Feb 28.

Department of Gastroenterology, Binzhou Medical University Hospital, Binzhou, Shandong, China.

Background: Gastric electrical pacing (GEP) could restore interstitial cells of Cajal in diabetic rats. M2 macrophages contribute to the repair of interstitial cells of Cajal injury though secreting heme oxygenase-1 (HO-1). The aim of the study is to investigate the effects and mechanisms of gastric electrical pacing on M2 macrophages in diabetic models.

Methods: Sixty male Sprague-Dawley rats were randomized into control, diabetic (DM), diabetic with the sham GEP (DM+SGEP), diabetic with GEP1 (5.5 cpm, 100 ms, 4 mA) (DM+GEP1), diabetic with GEP2 (5.5 cpm, 300 ms, 4 mA) (DM+GEP2), and diabetic with GEP3 (5.5 cpm, 550 ms, 4 mA) (DM+GEP3) groups. The apoptosis of interstitial cells of Cajal and the expression of macrophages were detected by immunofluorescence technique. The expression levels of the Nrf2/HO-1 and NF-B pathway were evaluated using western blot analysis or immunohistochemical method. Malonaldehyde, superoxide dismutase, and reactive oxygen species were tested to reflect the level of oxidative stress.

Results: Apoptosis of interstitial cells of Cajal was increased in the DM group but significantly decreased in the DM+GEP groups. The total number of macrophages was almost the same in each group. In the DM group, M1 macrophages were increased and M2 macrophages were decreased. However, M2 macrophages were dramatically increased and M1 macrophages were reduced in the DM+GEP groups. Gastric electrical pacing improved the Nrf2/HO-1 pathway and downregulated the phosphorylation of NF-B. In the DM group, the levels of malonaldehyde and reactive oxygen species were elevated and superoxide dismutase was lowered, while gastric electrical pacing reduced the levels of malonaldehyde and reactive oxygen species and improved superoxide dismutase.

Conclusion: Gastric electrical pacing reduces apoptosis of interstitial cells of Cajal though promoting M2 macrophages polarization to play an antioxidative stress effect in diabetic rats, which associates with the activated Nrf2/HO-1 pathway and the phosphorylation of NF-B pathway.
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http://dx.doi.org/10.1155/2021/1298657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937455PMC
May 2021

Multivariate correlation analysis of bio-accumulation with soil properties and potential health risks of cadmium and lead in rice seeds and cabbage in pollution zones, China.

Environ Geochem Health 2021 Sep 10;43(9):3485-3503. Epub 2021 Feb 10.

Department of Municipal & Environmental Engineering, School of Civil Engineering, Beijing Jiaotong University, Beijing, 100044, China.

A total of 475 representative cultivated land and 435 crop samples from 11 provinces of China were collected, and lead and cadmium in 6 polluted areas by wastewater irrigation and metallurgy industry were analyzed. Rice is the major cash crop and staple food of Chinese residents. Cabbage is also a common food in Chinese daily life. Pollution levels and spatial distribution of soil, rice, and cabbage samples were illustrated on the map. In individual or multiple areas, the multivariate correlation of heavy metal's (cadmium and lead) bio-accumulation in two kinds of plants (rice seed and cabbage) and soil properties (pH, cation exchange capacity, and organic matter) was also investigated. Spearman correlation analysis showed that soil pH values and organic matter (OM) had significant effects on the uptake of Cd and Pb in rice seed: the correlation between lg Cd BCF and pH values is -0.148* (p = 0.026), and the correlation between lg Pb BCF and pH values is -0.339** (p = 0.000). The cation exchange capacity (CEC) and pH significantly impact the Cd and Pb uptake in cabbage: the correlation between lg Cd EF and pH values is -0.199* (p = 0.040), and the correlation between lg Pb EF and pH values is -0.203** (p = 0.009). The Cd and Pb bio-concentration factor BCFs of rice and cabbage decreased with the increase of pH, CEC, and OM, except that Pb BCFs increased with the increase of OM in certain areas. The BCF of Cd varied positively from Pb in cabbage, but inversely with Pb in rice significantly at the 0.01 level (two-tailed Spearman correlation analysis). For the first time, the health quotient (HQ) of Cd and Pb in different regions was also calculated and illustrated on the map. In the soil samples of different areas, average HQ values of Cd and Pb in maturity varied from 0.0003-0.0023 to 0.0051-0.0460; average HQ values of immaturity were 0.0011-0.0103 and 0.0222-0.2014. In the rice samples of different areas, average HQ values of Cd and Pb in maturity varied from 0.305-1.360 to 0.027-0.321; average HQ values of immaturity were 0.601-2.678 and 0.053-0.633. Average HQ values orders of magnitude in the cabbage samples of different areas are the same as that of rice samples, and it is 2-4 orders higher than those in soil. Average HQ values of Cd and Pb in maturity varied from 0.152-1.354 to 0.006-0.506; average HQ values of immaturity were 0.510-4.192 and 0.022-0.207. The total HQ values ingested by children were all higher than those in adults. After investigation, it was found that the total HQ value of mature plants was also higher than that of immature plants. The results of this study would be of great help to future soil remediation with similar types.
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http://dx.doi.org/10.1007/s10653-021-00842-0DOI Listing
September 2021

Development of a two-in-one integrated assay for the analysis of circRNA-microRNA interactions.

Biosens Bioelectron 2021 Apr 23;178:113032. Epub 2021 Jan 23.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China. Electronic address:

The competitive endogenous RNA hypothesis is a new mechanism of RNA dialogue, in which circRNA-miRNA interaction (cmRRI) is found to be widely involved in the regulation of gene expression in tumors and other diseases. It is urgent but challenging to develop a convenient and efficient method to study the interaction between target circRNA and the candidate miRNAs. In this work, a biosensing method that allows directly analyzing cmRRI has been developed, so as to reveal the RNA dialogue strategy. The sensing system uses a bifunctional magnetic bead for the capture of target circRNA/miRNA complex as well as the signal amplification. Based on the nature of circRNA as a miRNA sponge, only if the target circRNAs and its regulatory miRNAs coexist as a complex, can the rolling circle amplification reaction be initiated to give a fluorescent signal as the output. Compared with traditional methods where the circRNA and its regulatory miRNAs have been separately analyzed, our design allows the integrated profiling of specific cmRRI by correlation characterization of two correlative RNAs, which represents a function-oriented method. The presented method also shows the analysis of the potential binding affinity of candidate miRNAs to target circRNAs. Furthermore, we have verified the ability of the sensor to directly detect cmRRI in biological samples, which reveals the promising applicability of this method for biomedical and clinical researches in the future.
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http://dx.doi.org/10.1016/j.bios.2021.113032DOI Listing
April 2021

Clinicopathological analysis of 5146 eyelid tumours and tumour-like lesions in an eye centre in South China, 2000-2018: a retrospective cohort study.

BMJ Open 2021 01 26;11(1):e041854. Epub 2021 Jan 26.

Department of Ophthalmology, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou, China

Objectives: To describe the clinicopathological characteristics of patients with eyelid tumours and tumour-like lesions in South China, investigate possible factors affecting tumour constitution.

Design: Retrospective cohort study.

Setting: All patients diagnosed with eyelid tumours were included from a high-volume tertiary eye care centre from South China which caring for over 2000 patients per day. All biopsied specimens were reviewed by two senior pathologists and were classified according to the fourth edition of the WHO Classification of Skin Tumours.

Participant: A total of 5146 cases of eyelid lesions were reviewed from 2000 to 2018, being classified by histogenesis and pathologic diagnosis, being compared with data from previous literature containing different races.

Main Outcome Measures: Age-specific and gender-specific incidence constitutions, time trends, tumour location, distribution in different age groups and relationship with Sociodmographic Index (SDI) were calculated.

Results: Benign tumours accounted for 85.08% (4378) of all cases, among which, nevus was most common (33.07%). Eight of top 10 benign lesions had higher occurrence in upper eyelids. The R² value of linear regression in patient annual number of benign lesions were 0.946 (p<0.01) for male and 0.914 (p<0.01) for female. More than 33.60% (1471/4378) were made up by patients younger than 40 years. The number of patients undergoing removal of benign lesions decreased with age. Among the malignant lesions, basal cell carcinoma (BCC) was most prevalent (48.70%), followed by sebaceous gland carcinoma (34.24%) and majority (81.8%) occurred in patients above 60 years.

Conclusions: Over the past 19 years, most eyelid tumours occurred at our centre were benign lesions. The number of patients presenting with benign lesions increased in both genders, especially among young females who were more likely to request surgeries. Among malignant lesions, BCC remains the most common type, appears a higher incidence in countries with higher SDI.
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http://dx.doi.org/10.1136/bmjopen-2020-041854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839916PMC
January 2021

Optimal concentration of ethylenediaminetetraacetic acid as an irrigation solution additive to reduce infection rates in rat models of contaminated wound.

Bone Joint Res 2021 Jan;10(1):68-76

Department of Orthopaedic Surgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, China.

Aims: In wound irrigation, 1 mM ethylenediaminetetraacetic acid (EDTA) is more efficacious than normal saline (NS) in removing bacteria from a contaminated wound. However, the optimal EDTA concentration remains unknown for different animal wound models.

Methods: The cell toxicity of different concentrations of EDTA dissolved in NS (EDTA-NS) was assessed by Cell Counting Kit-8 (CCK-8). Various concentrations of EDTA-NS irrigation solution were compared in three female Sprague-Dawley rat models: 1) a skin defect; 2) a bone exposed; and 3) a wound with an intra-articular implant. All three models were contaminated with or . EDTA was dissolved at a concentration of 0 (as control), 0.1, 0.5, 1, 2, 5, 10, 50, and 100 mM in sterile NS. Samples were collected from the wounds and cultured. The bacterial culture-positive rate (colony formation) and infection rate (pus formation) of each treatment group were compared after irrigation and debridement.

Results: Cell viability intervened below 10 mM concentrations of EDTA-NS showed no cytotoxicity. Concentrations of 1, 2, and 5 mM EDTA-NS had lower rates of infection and positive cultures for and compared with other concentrations in the skin defect model. For the bone exposed model, 0.5, 1, and 2 mM EDTA-NS had lower rates of infection and positive cultures. For intra-articular implant models 10 and 50 mM, EDTA-NS had the lowest rates of infection and positive cultures.

Conclusion: The concentrations of EDTA-NS below 10 mM are safe for irrigation. The optimal concentration of EDTA-NS varies by type of wound after experimental inoculation of three types of wound. Cite this article:  2021;10(1):68-76.
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http://dx.doi.org/10.1302/2046-3758.101.BJR-2020-0338.R1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7845470PMC
January 2021

Application of DGT/DIFS to assess bioavailable Cd to maize and its release in agricultural soils.

J Hazard Mater 2021 06 13;411:124837. Epub 2020 Dec 13.

Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Civil Engineering, Beijing Jiaotong University, Beijing 100044, China.

Tianjin, as an important maize production region in China, has a long history of sewage irrigation resulting in the soil cadmium (Cd) contamination. In this study, single extractions of CaCl and HNO, BCR sequential extraction and the diffusive gradients in thin films technique (DGT) were used to measure the bioavailable Cd content in soils. The Cd content in soil samples all exceeded the background values, with 14.3% and 33.3% of sites in the Baodi District (BDD) and Jinghai District (JHD) exceeding the risk control values, respectively. The average content of Cd in maize samples is lower than the pollution control values, which may be related to the higher pH (8.53) and organic matter (OM) content (15.01 g kg) in soils. Bioavailable Cd measured by DGT correlated well with Cd in maize grains (R =0.92). The DGT and DIFS model predicted the metals release from the agricultural lands, the total concentration of Cd in soil was relatively low, but the labile Cd in the soils has adequate metal release capability. This study shows that DGT is efficient in predicting Cd accumulation in grains from contaminated soils.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124837DOI Listing
June 2021

scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3.

Cell Mol Gastroenterol Hepatol 2021 11;11(5):1548-1592.e1. Epub 2021 Jan 11.

Department of Pediatrics, Abramson Research Center, Children's Hospital of Philadelphia Research Institute, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania. Electronic address:

Background And Aims: Bowel function requires coordinated activity of diverse enteric neuron subtypes. Our aim was to define gene expression in these neuron subtypes to facilitate development of novel therapeutic approaches to treat devastating enteric neuropathies, and to learn more about enteric nervous system function.

Methods: To identify subtype-specific genes, we performed single-nucleus RNA-seq on adult mouse and human colon myenteric plexus, and single-cell RNA-seq on E17.5 mouse ENS cells from whole bowel. We used immunohistochemistry, select mutant mice, and calcium imaging to validate and extend results.

Results: RNA-seq on 635 adult mouse colon myenteric neurons and 707 E17.5 neurons from whole bowel defined seven adult neuron subtypes, eight E17.5 neuron subtypes and hundreds of differentially expressed genes. Manually dissected human colon myenteric plexus yielded RNA-seq data from 48 neurons, 3798 glia, 5568 smooth muscle, 377 interstitial cells of Cajal, and 2153 macrophages. Immunohistochemistry demonstrated differential expression for BNC2, PBX3, SATB1, RBFOX1, TBX2, and TBX3 in enteric neuron subtypes. Conditional Tbx3 loss reduced NOS1-expressing myenteric neurons. Differential Gfra1 and Gfra2 expression coupled with calcium imaging revealed that GDNF and neurturin acutely and differentially regulate activity of ∼50% of myenteric neurons with distinct effects on smooth muscle contractions.

Conclusion: Single cell analyses defined genes differentially expressed in myenteric neuron subtypes and new roles for TBX3, GDNF and NRTN. These data facilitate molecular diagnostic studies and novel therapeutics for bowel motility disorders.
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http://dx.doi.org/10.1016/j.jcmgh.2020.12.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099699PMC
January 2021

A retrospective cohort study on prevalence of postoperative complications in comminuted patellar fractures: comparisons among stabilized with Cannulated-Screw, Kirschner-Wire, or Ring-Pin Tension Bands.

BMC Musculoskelet Disord 2021 Jan 11;22(1):60. Epub 2021 Jan 11.

Department of Orthopaedic Surgery, Shanghai Jiao Tong University affiliated Sixth People's Hospital, 200233, Shanghai, China.

Background: Displaced patellar fractures are commonly treated with open reduction and fixation with several different types of tension-band (TB) constructs. The main objective of this study was to compare the prevalence of postoperative complications after surgical stabilization of comminuted patellar fractures with either a modified Kirschner-wire tension band (MKTB), a cannulated-screw tension band (CSTB), or a ring-pin tension band (RPTB).

Methods: We conducted a retrospective and consecutive cohort study of comminuted patellar fractures (n = 334) stabilized using a TB construct. Postoperative premature loss of reduction, infection, and skin breakdown were compared according to the type of TB constructs received (MKTB, CSTB, or RPTB). The rate of implant removal due to symptomatic hardware was also evaluated.

Results: Fixation failure rate was significantly different among the groups (P = 0.013), with failure rates of 4.7% observed in the MKTB group,14.5% in the CSTB group, and 4.9% in the RPTB group. Skin breakdown and infection were not significantly different among the groups (Ps > 0.05). Due to symptomatic hardware, 40.5% of the patients in the MKTB group, 22.9% in the CSTB group, and 24.3% in the RPTB group underwent implant removal (P = 0.004). After adjusting for age, gender, comorbidities, number of supplementary screws/K-wires, and use of cerclage cables, multivariate regression analysis revealed that CSTB contributed to a 2.08-times greater risk of fixation failure compared to RPTB, while MKTB and RPTB were similar in risk of failure. In addition, it was found that patients who underwent MKTB fixation were more than twice as likely to undergo implant removal for symptomatic hardware compared with RPTB (odds ratio = 2.11, 95% CI = 1.20 to 3.72; P = 0.010).

Conclusions: RPTB have advantage over MKTB and CSTB fixation in terms of symptomatic hardware and premature failure, respectively.

Level Of Evidence: Therapeutic Level III.
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http://dx.doi.org/10.1186/s12891-020-03936-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798242PMC
January 2021

Asymmetrical arginine dimethylation of histone H4 by 8-oxog/OGG1/PRMT1 is essential for oxidative stress-induced transcription activation.

Free Radic Biol Med 2021 02 6;164:175-186. Epub 2021 Jan 6.

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wen Yuan Road, Nanjing, 210023, China. Electronic address:

It has been established that 8-oxoguanine DNA glycosylase 1 (OGG1) is the main enzyme removing oxidized guanine under oxidative stress. However, increasing evidence has shown that OGG1 is not only a base excision repair protein but also a new transcriptional coactivator involved in oxidative stress-induced gene expression. Its downstream target genes and the underlying regulatory mechanisms still need to be discerned. Here, it was discovered that c-Myc is a downstream target of OGG1 under oxidative stress and that H4R3me2a is involved in this transcriptional regulation. The increased level of H4R3me2a induced by HO is regulated by OGG1, which may directly interact with the specific arginine methyltransferase PRMT1 and promote the asymmetrical dimethylation of H4R3me1. H4R3me2a enrichment on the promoter of c-Myc can recruit YY1 and activate c-Myc transcription. Moreover, knocking down OGG1 or PRMT1 suppresses c-Myc transcription under oxidative stress by downregulating H4R3me2a formation. Furthermore, the overexpression of wild type (WT) H4R3 promotes c-Myc transcription, but the expression of mutant H4R3Q does not have this effect. Taken together, our data show that the 8-oxoG/OGG1/PRMT1/H4R3me2a/YY1 axis senses oxidative stress and promotes gene transcription.
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http://dx.doi.org/10.1016/j.freeradbiomed.2020.12.457DOI Listing
February 2021

Sarcopenia associated with 90-day readmission and overall survival after abdominal trauma.

Asia Pac J Clin Nutr 2020 ;29(4):724-731

Department of Intensive Care Unit, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Jiangsu Province, China. Email:

Background And Objectives: It is widely recognized that sarcopenia increases postoperative complications in trauma patients. However, the effects on prognosis remain unclear. This study aimed to evaluate the impact of sarcopenia on 90-day readmission and overall survival (OS) in abdominal trauma patients.

Methods And Study Design: 485 consecutive patients who underwent abdominal surgery after trauma in our institution were enrolled. Sarcopenia was diagnosed with low muscle mass and low muscle strength-handgrip. Multivariate logistic regression analysis was performed to identify factors that contributed to 90-day readmission and OS. Cox logistic regression analysis was used to assess the relationship between sarcopenia and OS.

Results: Sarcopenia was present in 120 of 485 patients (24.7%) with abdominal trauma within one week after admission based on the diagnostic cut-off values (40.9 cm2/m2 for men and 36.8 cm2/m2 for women). 90-day readmission was significantly higher in the sarcopenia group (p=0.019), and OS lower in the sarcopenia group (p=0.025). Sarcopenia was an independent predictor of 90-day readmission [odds ratio (OR): 5.34, 95% confidence interval (CI): 2.52-11.3].

Conclusions: Sarcopenia was associated with high 90-day readmission and low OS in abdominal trauma patients, and it was an independent risk factor for 90-day readmission.
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http://dx.doi.org/10.6133/apjcn.202012_29(4).0007DOI Listing
January 2020

Oxazolidinone-containing Hybrids with Antibacterial Activity Against Methicillin-resistant Staphylococcus aureus (MRSA): A Mini-review.

Curr Top Med Chem 2020 Nov 12. Epub 2020 Nov 12.

Hubei Key Laboratory of Radiation Chemistry and Functional Materials, School of Nuclear Technology and Chemistry & Biology, Hubei University of Science and Technology, Xian ning 437100, PR. China.

The increasing danger by methicillin-resistant Staphylococcus aureus (MRSA) and the limited therapeutic op-tions towards invasive MRSA infections make an urgent demand for the development of novel anti-MRSA agents. Oxazol-idinone derivatives could inhibit protein synthesis by acting on the ribosomal 50S subunit of the bacteria and prevent for-mation of a functional 70S initiation complex, so oxazolidinones are a novel class of antimicrobial agents with potential activity against a wide range of clinically significant multidrug-resistant Gram-positive pathogens. However, oxazolidinones such as linezolid are associated with significant adverse events, and myelosuppression represents the main unfavorable side effects. Moreover, MRSA isolates which are resistant to oxazolidinones have already emerged. Hybridization of oxazoli-dinone with other antibacterial pharmacophores has the potential to interact with multiple targets or to counterbalance the known side effects associated with each pharmacophore. Thus, oxazolidinone-containing hybrids are useful scaffolds for the development of novel anti-MRSA agents. This review covers the recent advances of oxazolidinone-containing hybrids with anti-MRSA activity developed in last decade to set up.
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http://dx.doi.org/10.2174/1568026620999201113104935DOI Listing
November 2020

Corrigendum to "Excretory Function of Intestinal Tract Enhanced in Kidney Impaired Rats Caused by Adenine".

ScientificWorldJournal 2020 19;2020:8273196. Epub 2020 Oct 19.

Kunming Key Laboratory of Molecular Biology for Sinomedicine, Faculty of Basic Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.

[This corrects the article DOI: 10.1155/2016/2695718.].
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http://dx.doi.org/10.1155/2020/8273196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603632PMC
October 2020

Ab initio investigation on the low-lying electronic states of thallium bromide.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Feb 25;246:118998. Epub 2020 Sep 25.

Institute of Atomic and Molecular physics, Sichuan University, Chengdu, China; Key Laboratory of High Energy Density Physics and Technology of Ministry of Education, Sichuan University, Chengdu, China. Electronic address:

Thirteen Λ-S states of TlBr molecule are calculated by the method of multireference configuration interaction (MRCI) plus Davidson correction (+Q), and the spectroscopic constants of these states are fitted. The dipole moment of thirteen Λ-S states are also included and analyzed in this calculation. Two bound states split into five Ω states with the SOC effect. The interaction between the molecular configurations of TlBr is analyzed with spin orbit coupling. Considering the forbidden transition, transition dipole moment with SOC effect is considered. On account of the accurate potential energy curves and the transition dipole moment, the Franck-Condon factors and radiative lifetime of the aΠ ↔ ΧΣ transition are calculated. The feasibility of laser cooling of TlBr molecule is analyzed by comparing with the thallium compounds and the bromides of the group-IIIA.
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http://dx.doi.org/10.1016/j.saa.2020.118998DOI Listing
February 2021

Circ_0005075 targeting miR-151a-3p promotes neuropathic pain in CCI rats via inducing NOTCH2 expression.

Gene 2021 Jan 27;767:145079. Epub 2020 Aug 27.

Department of Anesthesiology, Renmin Hospital, Hubei University of Medicine, No. 39 Chaoyang Middle Road, Maojian District, Shiyan City 442000, China. Electronic address:

Neuropathic pain is a most challenging diseases worldwide, caused by the injury of nerve system. CircularRNAs (circRNAs) are revealed to be involved in various diseases, includingneuropathic pain. However, the waycircRNAsparticipate in the progress ofneuropathic painstill needs further study. Identifyingthe possible circRNAexpression patterns of neuropathic painis of great significance to understand its underlying mechanism. Previously, circ_0005075 has been regarded as an important oncogene in multiple cancers and it has been characterized as an inflammation‑associated circRNA in various processes. Nevertheless, the functional role of circ_0005075 in neuropathic pain development is still poorly known. In our present study, we observed circ_0005075 was obviously increased in CCI rat models. Knockdown of circ_0005075 repressed thebehaviors of neuropathic pain including mechanical and thermal hyperalgesia. Moreover, loss of circ_0005075 could repress the neuroinflammation via targeting COX-2, IL-6 and TNF-α whereas inducing IL-10 in vivo. Additionally, we predicted miR-151a-3p as the potential target of circ_0005075 using bioinformatics analysis. We displayed that miR-151a-3p was greatly reduced in CCI rats and circ_0005075 reversed the repressive effect of miR-151a-3p on neuropathic pain. For another, NOTCH2 has been shown to induce a variety of intracellular responses correlated withneuropathic pain. Here, we found NOTCH2 expression was strongly induced in CCI rats and miR-151a-3p. In addition, circ_0005075 significantly rescued NOTCH2 expression, which could be repressed by miR-151a-3p. To sum up, we indicated that loss ofcirc_0005075relieved neuropathic pain progression by inducement of miR-151a-3p and inactivation of NOTCH2 signaling.
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http://dx.doi.org/10.1016/j.gene.2020.145079DOI Listing
January 2021
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