Publications by authors named "Tao Fan"

204 Publications

[Short-term effectiveness of femoral neck system in the treatment of femoral neck fracture].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 May;35(5):539-543

Orthopedics Center, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan Ningxia, 750001, P.R.China.

Objective: To investigate the short-term effectiveness of femoral neck system (FNS) in the treatment of femoral neck fracture.

Methods: The clinical data of 34 patients with femoral neck fracture admitted between January 2019 and April 2020 who met the selection criteria were retrospectively analyzed and divided into group A (19 patients were treated with conventional cannulated screw internal fixation) and group B (15 patients were treated with FNS internal fixation) according to the different methods of internal fixation. There was no significant difference between the two groups in general data such as gender, age, affected side, cause of injury, fracture type, and time from injury to operation ( >0.05). The operation time, intraoperative blood loss, fluoroscopy frequency, and fracture healing time were recorded and compared between the two groups. X-ray film and CT examinations were performed postoperatively to evaluate fracture reduction and internal fixation, and the shortening of the femoral neck on the affected side was measured compared with that on the healthy side. The Harris score was used to evaluate hip function.

Results: There was 1 unsatisfied reduction case in groups A and B respectively, the rest of the patients in both groups were obtained satisfied reduction. There was no significant difference in the quality of reduction between the two groups ( =-0.195, =0.854). There was no significant difference in operation time between the two groups ( =0.649, =0.064). The intraoperative blood loss in group A was significantly less than that in group B, and the fluoroscopy frequency was significantly more than that in group B, with significant differences ( <0.05). Except for 1 case in group A with screw out at 3 months after operation and no obvious callus formation, all fractures in the two groups reached clinical healing, and there was no significant difference in fracture healing time between the two groups ( =-0.127, =0.899). There was no necrosis of femoral head in the two groups. At last follow-up, there were 4 cases of femoral neck shortening in group A and 2 cases in group B. The hip function of both groups recovered well, and there was no significant difference in Harris score at last follow-up ( =0.956, =0.346).

Conclusion: The treatment of femoral neck fracture using FNS has less trauma. Compared with cannulated screw internal fixation, it can reduce the intraoperative fluoroscopy frequency and obtain satisfactory short-term effectiveness.
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http://dx.doi.org/10.7507/1002-1892.202012097DOI Listing
May 2021

Comprehensive analysis of a chemokine- and chemokine receptor family-based signature for patients with lung adenocarcinoma.

Cancer Immunol Immunother 2021 May 11. Epub 2021 May 11.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

The clinical significance and comprehensive features of chemokines and their receptors in lung adenocarcinoma (LUAD) have not been clarified. We aimed to characterize the expression profiles of chemokine and chemokine receptor family members and construct a chemokine- and chemokine receptor-based prognosis signature. A total of 1511 patients with LUAD from seven independent cohorts were included in the study. The training set collected from The Cancer Genome Atlas (TCGA) database containing 468 cases. The validation was performed on the basis of six different cohorts downloaded from Gene Expression Omnibus (GEO) database. A five-chemokine- and chemokine receptor-(CXCL2, CXCL13, CCL26, CCL20, CX3CR1) based prognosis signature was constructed with TCGA dataset using LASSO Cox regression and Cox proportional hazards regression analysis. A multivariate analysis verified that this signature was an independent prognostic factor. The predictive value of this signature was further verified by other six independent cohorts and multiple clinical subtypes. We performed immune cell infiltration analysis and biological pathway analysis which provided more insight into this signature-related immune and inflammatory landscape and clarified the intrinsic molecular mechanism by which this signature could be used to predict clinical prognosis. Furthermore, we explored the close relationship between this signature and tumor mutation burden (TMB), neoantigen burden, PD-1, PD-L1, CTLA4, TIDE score, T cell-inflamed score. This signature provided a robust prognostic biomarker for LUAD and could serve as a predictor for immunotherapy response, which may be used as an important supplement to immunotherapy to achieve individualized tumor treatment by optimizing the prognostic management and immunotherapy for patients with LUAD.
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http://dx.doi.org/10.1007/s00262-021-02944-1DOI Listing
May 2021

Merging N-Hydroxyphthalimide into Metal-Organic Frameworks for Highly Efficient and Environmentally Benign Aerobic Oxidation.

Chemistry 2021 May 10. Epub 2021 May 10.

Shanghai Key Lab of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, Shanghai, 200092, P. R. China.

Two highly efficient metal-organic framework catalysts TJU-68-NHPI and TJU-68-NDHPI have been successfully synthesized through solvothermal reactions of which the frameworks are merged with N-hydroxyphthalimide (NHPI) units, resulting in the decoration of pore surfaces with highly active nitroxyl catalytic sites. When t-butyl nitrite (TBN) is used as co-catalyst, the as-synthesized MOFs are demonstrated to be highly efficient and recyclable catalysts for a novel three-phase heterogeneous oxidation of activated C-H bond of primary and secondary alcohols, and benzyl compounds under mild conditions. Based on the high efficiency and selectivity, an environmentally benign system with good sustainability, mild conditions, simple work-up procedure has been established for practical oxidation of a wide range of substrates.
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http://dx.doi.org/10.1002/chem.202100987DOI Listing
May 2021

A Novel Immune-Related Prognostic Model for Response to Immunotherapy and Survival in Patients With Lung Adenocarcinoma.

Front Cell Dev Biol 2021 19;9:651406. Epub 2021 Mar 19.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Lung adenocarcinoma is one of the most malignant diseases worldwide. The immune checkpoint inhibitors targeting programmed cell death protein 1 (PD-1) and programmed cell death-ligand 1 (PD-L1) have changed the paradigm of lung cancer treatment; however, there are still patients who are resistant. Further exploration of the immune infiltration status of lung adenocarcinoma (LUAD) is necessary for better clinical management. In our study, the CIBERSORT method was used to calculate the infiltration status of 22 immune cells in LUAD patients from The Cancer Genome Atlas (TCGA). We clustered LUAD based on immune infiltration status by consensus clustering. The differentially expressed genes (DEGs) between cold and hot tumor group were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed. Last, we constructed a Cox regression model. We found that the infiltration of M0 macrophage cells and follicular helper T cells predicted an unfavorable overall survival of patients. Consensus clustering of 22 immune cells identified 5 clusters with different patterns of immune cells infiltration, stromal cells infiltration, and tumor purity. Based on the immune scores, we classified these five clusters into hot and cold tumors, which are different in transcription profiles. Hot tumors are enriched in cytokine-cytokine receptor interaction, while cold tumors are enriched in metabolic pathways. Based on the hub genes and prognostic-related genes, we developed a Cox regression model to predict the overall survival of patients with LUAD and validated in other three datasets. In conclusion, we developed an immune-related signature that can predict the prognosis of patients, which might facilitate the clinical application of immunotherapy in LUAD.
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http://dx.doi.org/10.3389/fcell.2021.651406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017122PMC
March 2021

Surgical management of a huge intramedullary ependymoma from oblongata to T4: A case report.

Int J Surg Case Rep 2021 Apr 11;81:105747. Epub 2021 Mar 11.

Spine Center, Sanbo Brain Hospital, Capital Medical University, Beijing, People's Republic of China. Electronic address:

Introduction And Importance: Huge intramedullary tumor is a rare condition. Surgical management of such huge ependymoma is technically challenging. We reported one case of 300 mm long intramedullary spinal cord ependymoma from oblongata to T4, which was satisfactorily en bloc gross total resected and the cervical spine alignment was successfully maintained by laminoplasty.

Case Presentation: A 30-year-old man presented to our service with two-year progressive neck pain combined with bilateral sense disorders of both the upper and lower extremities. The neurological grade was ASIA D. Repeat MRI imaging demonstrated a "medulla oblongata to T4" intramedullary spinal cord tumor complicated with cervical physiological curvature loss.

Clinical Discussion: Laminectomies were made at the C1-T4 level by a milling cutter. The tumor was gently separated bilaterally. The ventral part of the tumor was separated from the cranial and the caudal ends until reaching the middle site of the tumor. The whole tumor was isolated en bloc. Titanium miniplates were used bilaterally to fix both sides of the laminoplasty section on each level. At the 2-year postoperative follow-up, the muscle strength of both the upper and lower limbs were grade Ⅳ. No tumor recurrence was detected.

Conclusion: The total removal of an intramedullary ependymoma should be recommended as soon as the diagnosis is made, regardless of its extension. Long-level laminoplasty could be an effective alternative option to maintain the proper spine alignment.
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http://dx.doi.org/10.1016/j.ijscr.2021.105747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010453PMC
April 2021

Differences in the clinical characteristics and outcomes of COVID-19 patients in the epicenter and peripheral areas of the pandemic from China: a retrospective, large-sample, comparative analysis.

BMC Infect Dis 2021 Feb 24;21(1):206. Epub 2021 Feb 24.

Department of Respiratory and Critical Care Medicine, Clinical Research Center for Respiratory Disease, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.

Background: There is limited information on the difference in epidemiology, clinical characteristics and outcomes of the initial outbreak of the coronavirus disease (COVID-19) in Wuhan (the epicenter) and Sichuan (the peripheral area) in the early phase of the COVID-19 pandemic. This study was conducted to investigate the differences in the epidemiological and clinical characteristics of patients with COVID-19 between the epicenter and peripheral areas of pandemic and thereby generate information that would be potentially helpful in formulating clinical practice recommendations to tackle the COVID-19 pandemic.

Methods: The Sichuan & Wuhan Collaboration Research Group for COVID-19 established two retrospective cohorts that separately reflect the epicenter and peripheral area during the early pandemic. The epidemiology, clinical characteristics and outcomes of patients in the two groups were compared. Multivariate regression analyses were used to estimate the adjusted odds ratios (aOR) with regard to the outcomes.

Results: The Wuhan (epicenter) cohort included 710 randomly selected patients, and the peripheral (Sichuan) cohort included 474 consecutive patients. A higher proportion of patients from the periphery had upper airway symptoms, whereas a lower proportion of patients in the epicenter had lower airway symptoms and comorbidities. Patients in the epicenter had a higher risk of death (aOR=7.64), intensive care unit (ICU) admission (aOR=1.66), delayed time from illness onset to hospital and ICU admission (aOR=6.29 and aOR=8.03, respectively), and prolonged duration of viral shedding (aOR=1.64).

Conclusions: The worse outcomes in the epicenter could be explained by the prolonged time from illness onset to hospital and ICU admission. This could potentially have been associated with elevated systemic inflammation secondary to organ dysfunction and prolonged duration of virus shedding independent of age and comorbidities. Thus, early supportive care could achieve better clinical outcomes.
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http://dx.doi.org/10.1186/s12879-020-05728-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903397PMC
February 2021

The Effect of Hydrothermal Treatment on Structure and Flotation Characteristics of Lignite and a Mechanistic Analysis.

ACS Omega 2021 Jan 12;6(3):1930-1940. Epub 2021 Jan 12.

School of Chemical and Environmental Engineering, China University of Mining & Technology (Beijing), Beijing 100083, China.

Lignite is difficult to obtain highly efficient indexes by conventional flotation due to its poor surface hydrophobicity. Although the modification of flotation reagents has made some progress in improving the flotation performance, they all remain at the stage in the laboratory. Here, we proposed to improve the flotation performance by hydrothermal treatment dewatering (HTD) for lignite. Combined with the C NMR and FT-IR analysis, the impact of the HTD process on lignite's chemical structural evolution and flotation performance was investigated. The results showed that the HTD process is an effective means for dehydration and deoxygenation to increase lignite quality and the metamorphic degree of coal. The content of oxygen-containing functional groups generally decreases during the HTD process, especially carboxyl acid and ether groups. Therefore, surface properties and wettability of HTD coal samples were changed, and the contact angle gradually increases with the HTD temperature increased, which enhance the hydrophobicity and decrease hydrophilia of the lignite surface. The HTD process effectively improves the flotation performance of lignite during the conventional flotation operation condition, even if the coal pulp after HTD was directly used as a flotation feedstock. Specifically, the coal oil and capryl alcohol were used as collector and forming agents, respectively, and the pulp concentration was adjusted to 60 g/L. The yield of the cleaned coal increased from 12.14% of the raw coal to 55.58% of HTD310, and combustible matter recovery increased from 13.83% of the raw coal to 65.17% of HTD310 by raw coal basis.
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http://dx.doi.org/10.1021/acsomega.0c04713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841791PMC
January 2021

Identification of Immune-Related Prognostic Biomarkers Associated with HPV-Positive Head and Neck Squamous Cell Carcinoma.

J Immunol Res 2021 8;2021:6661625. Epub 2021 Jan 8.

Department of Otolaryngology Head and Neck Surgery, Affiliated Changsha Hospital of Hunan Normal University, 70 Lushan Road, Changsha, Hunan 410013, China.

Background: As a type of malignant tumor, head and neck squamous cell carcinoma (HNSCC) seriously threatens human health. This study is aimed at constructing a new, reliable prognostic model.

Method: The gene expression profile data of HNSCC patients were downloaded from the Gene Expression Omnibus and The Cancer Genome Atlas databases. The immune-related differentially expressed genes (IRDEGs) related to HNSCC were identified. We then used Cox regression analysis and least absolute shrinkage and selection operator (LASSO) analysis to explore IRDEGs related to the HNSCC prognosis and to construct and validate a risk scoring model and used ESTIMATE to evaluate tumor immune infiltration in HNSCC patients. Finally, we validated IGSF5 expression and function in HNSCC cells.

Results: A total of 1,195 IRDEGs were found from the GSE65858 dataset. Thirty-one of the 1,195 IRDEGs were associated with the prognosis of HNSCC. Nine key IRDEGs were further selected using the LASSO method, and a risk scoring model was established for predicting the survival of HNSCC patients. According to the risk scoring model, the prognosis of patients in the high-risk group was worse than that of the low-risk group; the high-risk group had significantly higher immune scores than the low-risk group; and between the high- and low-risk samples, there were significant differences in the proportion of 10 types of cells, including naive cells, plasma cells, and resting CD4 memory T cells. IGSF5 has low expression in HNSCC, and overexpression of IGSF5 significantly impaired HNSCC cell proliferation.

Conclusion: This prognostic risk assessment model can help systematically evaluate the survival prognosis of HNSCC patients and provides a new research direction for the improvement of the survival prognosis of HNSCC patients in clinical practice.
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http://dx.doi.org/10.1155/2021/6661625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810542PMC
January 2021

A network pharmacology perspective for deciphering potential mechanisms of action of Solanum nigrum L. in bladder cancer.

BMC Complement Med Ther 2021 Jan 25;21(1):45. Epub 2021 Jan 25.

Department of Urology, XuZhou Central Hospital Affiliated to Nanjing University of Chinese Medicine, Jiefang South Road, No. 199, Jiangsu, Xuzhou, China.

Background: Solanum nigrum L. decoction has been used as a folklore medicine in China to prevent the postoperative recurrence of bladder cancer (BC). However, there are no previous pharmacological studies on the protective mechanisms of this activity of the plant. Thus, this study aimed to perform a systematic analysis and to predict the potential action mechanisms underlying S. nigrum activity in BC based on network pharmacology.

Methods: Based on network pharmacology, the active ingredients of S. nigrum and the corresponding targets were identified using the Traditional Chinese Medicines for Systems Pharmacology Database and Analysis Platform database, and BC-related genes were screened using GeneCards and the Online Mendelian Inheritance in Man database. In addition, ingredient-target (I-T) and protein-protein interaction (PPI) networks were constructed using STRING and Cytoscape, Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted, and then the pathways directly related to BC were integrated manually to reveal the pharmacological mechanism underlying S. nigrum-medicated therapeutic effects in BC.

Results: Seven active herbal ingredients from 39 components of S. nigrum were identified, which shared 77 common target genes related to BC. I-T network analysis revealed that quercetin was associated with all targets and that NCOA2 was targeted by four ingredients. Besides, interleukin 6 had the highest degree value in the PPI network, indicating a hub role. A subsequent gene enrichment analysis yielded 86 significant GO terms and 89 significant pathways, implying that S. nigrum had therapeutic benefits in BC through multi-pathway effects, including the HIF-1, TNF, P53, MAPK, PI3K/Akt, apoptosis and bladder cancer pathway.

Conclusions: S. nigrum may mediate pharmacological effects in BC through multi-target and various signaling pathways. Further validation is required experimentally. Network pharmacology approach provides a predicative novel strategy to reveal the holistic mechanism of action of herbs.
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http://dx.doi.org/10.1186/s12906-021-03215-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836472PMC
January 2021

Long-level intramedullary spinal cord astrocytoma complicated with spine scoliosis: Report of two cases.

Int J Surg Case Rep 2021 Feb 15;79:234-238. Epub 2021 Jan 15.

Spine Center, Sanbo Brain Hospital, Capital Medical University, Beijing, People's Republic of China. Electronic address:

Introduction And Importance: Long-level intramedullary astrocytomas complicated with spine scoliosis are rare. Surgical treatment of such tumors becomes more complicated and challenging when spinal scoliosis is present. However, studies describing the treatment of long segmental intramedullary spinal cord astrocytomas complicated with severe spine scoliosis have been rarely reported.

Case Presentation: Two cases of long-level intramedullary astrocytomas complicated with severe spine scoliosis were surgically treated with one-stage operation of tumor resection and scoliosis correction in this report. Case 1: A 16-year-old boy presented to our hospital with a five-month progressive paresthesia, weakness of the left lower limb, and a long-time abnormal body appearance. MRI showed a T4-T12 intramedullary tumor combined with spinal scoliosis. Case 2: A 14-year-old boy presented at our service with a 6-year history of visible spine scoliosis and a 1-year progressive motor disability of bilateral lower limbs. Spine MRI indicated a long-level abnormal syringomyelia signal from C4 to L1 and there was irregular enhancement after intravenous contrast medium administration at C7-T2 and T9-T12 level.

Discussion: We performed a laminectomy over the whole length of the tumor and corrected the scoliosis with trans-pedicle screws. The patients exhibited a long-time tumor free with largely neurological function preservation. One-stage operation did not generate severe short- or long-term complications. The correction of the scoliosis prevented the progression of the spinal deformity and facilitated the recovery of normal life.

Conclusion: This case report demonstrates that the one-stage resection of long-level intramedullary astrocytoma and correction of the complicated scoliosis might be a feasible option.
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http://dx.doi.org/10.1016/j.ijscr.2021.01.035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820800PMC
February 2021

The therapeutic significance of the novel photodynamic material TPE-IQ-2O in tumors.

Aging (Albany NY) 2020 12 19;13(1):1383-1409. Epub 2020 Dec 19.

Department of Thoracic Surgery, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Combination therapies based on photodynamic therapy (PDT) have received much attention in various cancers due to their strong therapeutic effects. Here, we aimed to explore the safety and effectiveness of a new mitochondria-targeting photodynamic material, TPE-IQ-2O, in combination therapies (combined with surgery or immunotherapy). The safety and effectiveness of TPE-IQ-2O PDT were verified with cytotoxicity evaluation and a zebrafish xenograft model , respectively. The effectiveness of TPE-IQ-2O PDT combined with surgery or immune checkpoint inhibitors (ICIs) was verified in tumor-bearing mice. Small animal imaging, immunohistochemistry, and flow cytometry were used to determine the underlying mechanism. TPE-IQ-2O PDT can not only reduce tumor recurrence in surgical treatment but also effectively improve the response to ICIs in immunotherapy without obvious toxicity. It was also found to ameliorate the immunosuppressive tumor microenvironment and promote the antitumor immunity induced by ICIs by increasing CD8 tumor-infiltrating lymphocyte accumulation. Thus, TPE-IQ-2O PDT is a safe and effective antitumor therapy that can be combined with surgery or immunotherapy.
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http://dx.doi.org/10.18632/aging.202355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835032PMC
December 2020

The prognostic value of tumor-stromal ratio combined with TNM staging system in esophagus squamous cell carcinoma.

J Cancer 2021 1;12(4):1105-1114. Epub 2021 Jan 1.

Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Tumor stroma is a crucial component of the tumor environment that interacted with tumor cells and modulated tumor cell proliferation, immune evasion, and metastasis. Tumor-stromal ratio (TSR) has been confirmed as an influential independent prognostic factor for diverse types of cancer, but it was seldom discussed in esophagus squamous cell carcinoma (ESCC). In present study, pathological sections from the most invasive part of the ESCC of 270 patients were analyzed for their TSR by visual inspection and software. The TSR was combined with the TNM staging system to further explain its predictive value of prognosis. The 57 cases ESCC from TCGA database also were included as an independently validated cohort. Our results indicated that TSR was a robust prognostic factor for ESCC patients. TSR by visual inspection was dependable to reflect the stroma percent of the tumor compared to software calculation. Compared with stroma-low groups, the risk of death increased by 153.1% for patients in the stroma-high group [HR=2.531 (95%CI 1.657-3.867), <0.001]. The results of ROC analysis in two cohorts indicated that TSNM staging system had better resolving ability with the largest area under the curve [0.698 95%CI (0.635-0.760), 0.691 95%CI (0.555-0.807)], compare to TNM. The novel TSNM staging system revealed strong predictive performance (<0.001). TSR was a reliable dependent indicator for ESCC prognosis. The TSNM staging system has a better discriminative ability than the conventional TNM staging system, especially for III stage patients.
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http://dx.doi.org/10.7150/jca.50439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797665PMC
January 2021

Utilization of a Wheat55K SNP array-derived high-density genetic map for high-resolution mapping of quantitative trait loci for important kernel-related traits in common wheat.

Theor Appl Genet 2021 Mar 3;134(3):807-821. Epub 2021 Jan 3.

College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China.

Key Message: This study mapped QTLs associated with kernel-related traits by high-density genetic map. Five new major and stable QTLs for KL, KDR, SN, and KWPS were mapped in multiple environments. In the present study, a recombinant inbred line population including 371 lines derived from the cross of Chuannong18 and T1208 was genotyped using the Wheat55K single nucleotide polymorphism array. A novel high-density genetic map consisting of 11,583 markers spanning 4192.62 cM and distributed across 21 wheat chromosomes was constructed. QTLs for important kernel-related traits were mapped in multiple environments. A total of 96 and 151 QTLs were mapped by using the ICIM method and the MET method, respectively. And a total of 114 digenic epistatic QTLs were also detected across 21 chromosomes, and the epistatic effects of each trait were analyzed. BLAST analysis showed that 23 QTLs for different kernel-related traits were first time mapped and five of them were major and stable QTLs for kernel diameter ratio (121.34-126.83 cM on 4BS), spike number per square meter (71.32-73.84 cM on 2DS), kernel weight per spike (71.32-75.26 cM on 2DS), and kernel length (16.78-31.64 cM on 6A and 51.63-58.40 cM on 3D), respectively. Fifteen QTL clusters that contained 58 QTLs were also detected, and all most stable QTLs were contained in these QTL clusters. Significant correlations between different traits were detected and discussed. These results lay the foundation for fine mapping and cloning of the gene(s) underlying the stable QTLs detected in this study.
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http://dx.doi.org/10.1007/s00122-020-03732-8DOI Listing
March 2021

The alteration and potential relationship of vaginal microbiota and chemokines for unexplained recurrent spontaneous abortion.

Medicine (Baltimore) 2020 Dec;99(51):e23558

Department of Obstetrics, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, 518003.

Abstract: The diagnosis and treatment of unexplained recurrent spontaneous abortion (URSA) is an important and hot topic in the field of obstetrics and gynecology. During our clinical investigation (observation), we have found that URSA patients usually experience recurrent vaginitis or vaginal dysbacteriosis during periods of non-pregnancy, pregnancy, and post-abortion. However, there is no research on vaginal dysbacteriosis's influence on URSA. Using women with normal induced abortion as a control group, and using 16S rRNA sequencing, which helps to screen differentially expressed flora, this study discusses the relevance between differential bacteria at the genus level and the incidence of URSA. Another aim of this study is to determine whether certain pathogenic genera can cause an imbalance in immune tolerance of the maternal and fetal interface through regulatory chemokines, which leads to recurrent spontaneous abortion. This article has explored URSA pathogenesis from the perspective of differentially expressed vaginal flora, which has great theoretical significance for the early diagnosis and treatment of URSA.
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http://dx.doi.org/10.1097/MD.0000000000023558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748213PMC
December 2020

MicroRNA-22 enhances the differentiation of mouse induced pluripotent stem cells into alveolar epithelial type II cells.

Eur J Histochem 2020 Oct 1;64(s2). Epub 2020 Oct 1.

Department of Traditional Chinese Medicine, Wuhan Fifth Hospital, Wuhan .

Considerable evidence has verified that microRNAs (miRNAs) play important roles in various cellular processes including differentiation. However, the regulatory roles of miRNAs involved in the differentiation of induced pluripotent stem cells (iPSC) into lung epithelial cells are still unknown. In this study, we first evaluated the current protocols to differentiate iPSC into alveolar epithelial type II (AEC II) cells, but the efficiency is low. We next identified that miR-22 can efficiently enhance the differentiation of iPSC into AEC II cells under the stimulation of proper growth factors and growing on appropriate matrix. Moreover, the AEC II cells generated from iPSC with miR-22 overexpression can proliferate and secrete lung surfactant. Here, we discovered a previously unknown interaction between miR-22 and iPSC differentiation but also provide a potential target for the effective derivation of AEC II from iPSCs for cell-based therapy.
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http://dx.doi.org/10.4081/ejh.2020.3170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542669PMC
October 2020

Immune profile of the tumor microenvironment and the identification of a four-gene signature for lung adenocarcinoma.

Aging (Albany NY) 2020 12 9;13(2):2397-2417. Epub 2020 Dec 9.

Department of Oncology, Renmin Hospital of Wuhan University, Wuhan 430060, China.

The composition and relative abundances of immune cells in the tumor microenvironment are key factors affecting the progression of lung adenocarcinomas (LUADs) and the efficacy of immunotherapy. Using the cancer gene expression dataset from The Cancer Genome Atlas (TCGA) program, we scored stromal and immune cells for tumor purity prediction by CIBERSORT and ESTMATE. Differential expression analysis was employed to identify 374 genes between the high-score group and the low-score group, which were utilized to conduct Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Protein-protein interaction (PPI) and Cox regression analysis were performed on the differentially expressed genes (DEGs) to identify four key tumor microenvironment (TME) -related genes (CCR2, CCR4, P2RY12, and P2RY13). The expression levels of the four DEGs differed significantly among LUAD patients of different ages, genders, and TNM stages. We found that the infiltration of resting memory CD4+ T cells, memory B cells, and M0 macrophages into the TME was co-regulated by these four DEGs. These four genes were closely related to the prognosis of LUAD and affected the infiltration of immune cells into the TME, which had predictive prognostic value in LUAD.
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http://dx.doi.org/10.18632/aging.202269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880407PMC
December 2020

Numerical Analysis and Theoretical Verification on the Dynamic Response of Typical Components under Explosion Load.

ACS Omega 2020 Nov 20;5(43):28351-28359. Epub 2020 Oct 20.

State Key Laboratory of Explosion Science and Technology, Beijing Institute of Technology, Beijing 100081, China.

To analyze the response of float glass under explosion load, the model was developed by the method of FEM simulation combined with stress theory. The numerical simulation results show that the stress caused by the blast wave on the center of the glass panel is continuous, while the stress around the glass is instantaneous. The displacement on both sides of the glass is larger than that at the center and at the four corners of the glass. The effective stress at the center of the glass is 86.08 MPa by numerical simulation. However, the effective stress at the center of the glass is 87.75 MPa by theoretical calculation. The error rate between the simulation result and the theoretical result is only 1.90%. Therefore, it is proved that the theoretical expressions in this paper are reliable for calculating the effective stress of single float glass under explosion load.
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http://dx.doi.org/10.1021/acsomega.0c04355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643307PMC
November 2020

Preparation of an Intelligent Oleophobic Hydrogel and Its Application in the Replacement of Locally Damaged Oil Pipelines.

ACS Appl Mater Interfaces 2020 Nov 6;12(46):52018-52027. Epub 2020 Nov 6.

State Key Laboratory of Explosion Science and Technology, Beijing Institute of Technology, Beijing 100081, China.

In the pipeline transportation process for crude oil, the most important and popular maintenance method for perforated and ruptured oil pipelines is the replacement of the damaged pipeline segment. However, this method has several disadvantages, including a complex process, large time consumption, and excessively high costs. The present study reported the preparation of a strong cross-linking hydrogel that served as a temporary blocking material during the long-distance oil pipeline partial replacement process. The prepared product was characterized by infrared spectroscopy, X-ray diffraction analysis, and scanning electron microscopy to analyze the microscopic reactions and structures. Orthogonal experiments for shear stress were performed to determine the optimal synthesis condition. The relevant experiments indicated that the proposed product can effectively isolate oil and oil gas, and a 4.5 m long hydrogel can resist the force of a 0.57 MPa overpressure. The blocked pipeline turned to a dredged state on changing the pipeline pressure. The flame resistance experiment showed that the hydrogel exhibited excellent flame resistance and could therefore ensure the safety of the hot work. On the basis of this hydrogel material, a new method for replacing the partially damaged oil pipeline was proposed.
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http://dx.doi.org/10.1021/acsami.0c15890DOI Listing
November 2020

Microarray analysis of aberrant microRNA expression patterns in spinal cord gliomas of different grades.

Oncol Lett 2020 Dec 21;20(6):371. Epub 2020 Oct 21.

Department of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing 100093, P.R. China.

MicroRNAs (miRNAs) are involved in the development of several types of tumor; however, their role in spinal gliomas remains unknown. The present study aimed to identify potentially novel spinal cord gliomas (SCG)-associated miRNAs and to characterize their roles in the development and progression of SCG. miRNA expression levels in low-grade SCG (classed as stage I-II SCG based on the World Health Organization grading system), high-grade SCG (classed as stage IV SCG based on the World Health Organization grading system) and 5 control cases were measured using a miRNA expression microarray. Subsequently, blood samples from the spinal cord of patients with differing grades of SCG were screened for differentially expressed miRNAs (DEmiRNAs). Compared with the control group, 7 upregulated and 36 downregulated miRNAs were identified in the low-grade SCG group and a total of 70 upregulated and 20 downregulated miRNAs were identified in the high-grade SCG group (P≤0.05, fold change >2). Gene Ontology analysis revealed that the regulation of cellular metabolic processes, negative regulation of biological processes and axon guidance were primarily involved. Moreover, pathway analysis showed that the target genes of DEmiRNAs were enriched in tumor-related signaling pathways, such as the MAPK and Wnt signaling pathway. The results suggest that DEmiRNAs in peripheral blood may serve as novel target markers with high specificity and sensitivity for the diagnosis of SCG.
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http://dx.doi.org/10.3892/ol.2020.12234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640765PMC
December 2020

Comparison of clinical characteristics of COVID-19 between elderly patients and young patients: a study based on a 28-day follow-up.

Aging (Albany NY) 2020 10 26;12(20):19898-19910. Epub 2020 Oct 26.

Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.

The number of corona virus disease 2019 cases is increasing rapidly. However, the comparison of clinical characteristics between patients ≥ 70 and those < 70 has not been implemented yet. To achieve that, we collected clinical data of consecutive 222 patients in Renmin Hospital of Wuhan University diagnosed between January 13, 2020 and February 4, 2020. We divided them into an under-70 group and an over-70 group according to their ages, comparing their clinical characteristics. Meanwhile, univariate and multivariate Cox regression analyses were performed to identify the prognostic factors. Among the patients enrolled, 37 (16.67%) were 70 or older and 185 (83.33%) were younger than 70. Higher proportions of dyspnoea, expectoration, chronic cardiovascular disease, diabetes, organ complications, severe-to-critical cases, a higher death rate, a longer hospital stay and decreased immune status were observed in the over-70 group patients compared with their younger counterparts. The risk factors for death included dyspnoea, muscle ache, elevated myocardial enzymes, elevated C3 in over-70 patients and dyspnoea, pharyngalgia, chronic cardiac disease, increased C-reactive protein, IgA, decreased platelets in under-70 patients. Overall, our research compared the clinical characteristics of the two populations with different immune status and illustrated differentiated risk factors for death in them.
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http://dx.doi.org/10.18632/aging.104077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655161PMC
October 2020

Identification of Key Genes Involved in Acute Myocardial Infarction by Comparative Transcriptome Analysis.

Biomed Res Int 2020 6;2020:1470867. Epub 2020 Oct 6.

The Department of Cardiovascular Medicine, The Second People's Hospital of Changshu, Jiangsu, China.

Background: Acute myocardial infarction (AMI) is regarded as an urgent clinical entity, and identification of differentially expressed genes, lncRNAs, and altered pathways shall provide new insight into the molecular mechanisms behind AMI.

Materials And Methods: Microarray data was collected to identify key genes and lncRNAs involved in AMI pathogenesis. The differential expression analysis and gene set enrichment analysis (GSEA) were employed to identify the upregulated and downregulated genes and pathways in AMI. The protein-protein interaction network and protein-RNA interaction analysis were utilized to reveal key long noncoding RNAs.

Results: In the present study, we utilized gene expression profiles of circulating endothelial cells (CEC) from 49 patients of AMI and 50 controls and identified a total of 552 differentially expressed genes (DEGs). Based on these DEGs, we also observed that inflammatory response-related genes and pathways were highly upregulated in AMI. Mapping the DEGs to the protein-protein interaction (PPI) network and identifying the subnetworks, we found that and were the hub nodes of two subnetworks with the highest connectivity, which were found to be involved in circadian rhythm and organ- or tissue-specific immune response. Furthermore, 23 lncRNAs were differentially expressed between AMI and control groups. Specifically, we identified some functional lncRNAs, including and its antisense RNA, , and three lncRNAs (, , and ), which were predicted to be interacting with and participate in Toll-like receptor signaling pathway. In addition, we also employed the MMPC algorithm to identify six gene signatures for AMI diagnosis. Particularly, the multivariable SVM model based on the six genes has achieved a satisfying performance (AUC = 0.97).

Conclusion: In conclusion, we have identified key regulatory lncRNAs implicated in AMI, which not only deepens our understanding of the lncRNA-related molecular mechanism of AMI but also provides computationally predicted regulatory lncRNAs for AMI researchers.
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http://dx.doi.org/10.1155/2020/1470867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559508PMC
May 2021

Exosome-Derived LncRNAs in Lung Cancer.

Front Oncol 2020 23;10:1728. Epub 2020 Sep 23.

Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, China.

As extracellular vesicles, exosomes are released from most cells to perform cell-cell communication. Recent studies have shown that exosomes could be released into tumor microenvironment and blood to promote tumor progression through packaging and transmitting various bioactive molecules, such as cholesterol, proteins, lipids, miRNAs, mRNAs, and long non-coding RNAs (lncRNAs) to distant cells. LncRNAs have emerged as a major class of non-coding transcripts. A lot of LncRNAs have been discovered during the past few years of research on genomics. They have been proven to participate in various biological functions and disease processes through multiple mechanisms. In this review, we analyzed the role of exosome-derived lncRNAs in lung carcinogenesis and metastasis. We also highlight opportunities for the clinical potential of exosomes with specific lncRNAs as biomarkers and therapeutic intervention in lung cancer.
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http://dx.doi.org/10.3389/fonc.2020.01728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538687PMC
September 2020

Subsurface drip irrigation reduces cadmium accumulation of pepper (Capsicum annuum L.) plants in upland soil.

Sci Total Environ 2021 Feb 1;755(Pt 2):142650. Epub 2020 Oct 1.

Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China; National Research and Observation Station of Shangqiu Agro-ecology System, Shangqiu 476000, China. Electronic address:

Given that Cd pollution in dry land has aroused wide public concern, numerous remediation technologies has been utilized, yet there are limited cost-effective techniques that do not affect the original planting patterns. Fortunately, irrigation management can meet these requirements, while the effects of irrigation practices on Cd uptake by crops in slightly Cd-polluted upland soil remain elusive. Here, we aimed to investigate how the irrigation methods altered the Cd availability in soil, Cd accumulation in plants, microorganism population in soil, root morphology, and enzyme activities in soil and plants. We examined three irrigation treatments - surface drip irrigation (DI), subsurface drip irrigation (SDI), alternate-rows irrigation (ARI), and the control conventional furrow irrigation (CFI). The results showed that SDI remarkably reduced Cd content in roots, shoots and fruits, increased yield, and improved root growth and activity in soil of 20-40 cm compared to other treatments, though the Cd concentration in rhizosphere was not decreased significantly. The microbial population and enzyme activities in rhizosphere and enzyme activities in leaves and roots in SDI and ARI were basically higher than DI and CFI. Therefore, SDI has the prominent potential to reduce Cd uptake by crops in upland soil polluted with low Cd.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142650DOI Listing
February 2021

Analysis of the clinical characteristics of 77 COVID-19 deaths.

Sci Rep 2020 10 2;10(1):16384. Epub 2020 Oct 2.

Department of Pulmonary and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, People's Republic of China.

The COVID-19 outbreak is becoming a public health emergency. Data are limited on the clinical characteristics and causes of death. A retrospective analysis of COVID-19 deaths were performed for patients' clinical characteristics, laboratory results, and causes of death. In total, 56 patients (72.7%) of the decedents (male-female ratio 51:26, mean age 71 ± 13, mean survival time 17.4 ± 8.4 days) had comorbidities. Acute respiratory failure (ARF) and sepsis were the main causes of death. Increases in C-reactive protein (CRP), lactate dehydrogenase (LDH), D-dimer and lactic acid and decreases in lymphocytes were common laboratory results. Intergroup analysis showed that (1) most female decedents had cough and diabetes. (2) The proportion of young- and middle-aged deaths was higher than elderly deaths for males, while elderly decedents were more prone to myocardial injury and elevated CRP. (3) CRP and LDH increased and cluster of differentiation (CD) 4+ and CD8+ cells decreased significantly in patients with hypertension. The majority of COVID-19 decedents are male, especially elderly people with comorbidities. The main causes of death are ARF and sepsis. Most female decedents have cough and diabetes. Myocardial injury is common in elderly decedents. Patients with hypertension are prone to an increased inflammatory index, tissue hypoxia and cellular immune injury.
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http://dx.doi.org/10.1038/s41598-020-73136-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532142PMC
October 2020

Research of New Cross-Flow Technology with a Gas Heat Carrier for Lignite Pyrolysis.

ACS Omega 2020 Sep 4;5(37):23876-23884. Epub 2020 Sep 4.

School of Chemical & Environmental Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China.

The adaptability of the gas heat carrier cross-flow pyrolysis process to Huolinhe lignite (0-80 mm) and the influence of the temperature of the heat carrier and moisture of the raw materials on the yield and properties of the pyrolysis products were studied using a 30 t/d cross-flow pyrolysis pilot plant with a gas heat carrier. When the temperature of the gas heat carrier was increased from 750 to 850 °C, the volatiles () produced from the char decreased from 16.81 to 10.57%, the yield of tar () increased from 73.81 to 81.1% of the Gray-King (G-K) tar yield, and the yield of gas decreased from 1312 to 1205 Nm/t. When the temperature of the gas heat carrier was constant at 850 °C and the moisture content () of lignite was reduced from 12.49 to 7.14%, the of char declined from 10.57 to 6.59%, the yield of gas was reduced from 1205 to 805 Nm/t, the calorific value of gas increased from 3.88 to 4.68 MJ/m, and the increased to 81.56% of the G-K tar yield. The light tar content (boiling point below 360 °C) was more than 60%, and the ash content was less than 0.04%. The cross-flow pyrolysis moving bed could effectively treat full-size lignite, afforded continuous and stable operation, and provided a high oil and gas yield, which provides basic data for further industrial design.
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http://dx.doi.org/10.1021/acsomega.0c03109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513340PMC
September 2020

Ultrasound-guided suprainguinal fascia iliaca block combined with a sacral plexus block for lower extremity surgery: A case report.

Medicine (Baltimore) 2020 Aug;99(35):e21921

Hangzhou Red Cross Hospital, Anesthesiology Department.

Rationale: The anesthetic management of patients with severe pulmonary hypertension is different from that of normal, healthy patients, and regional nerve blocks are commonly used for them. Due to the individual variability of the course, distribution, and branching of the nerves below the inguinal ligament, the supra-inguinal fascia iliaca (SIFI) block has a wider and more stable blocking area. In combination with the sacral plexus block, they can satisfy the needs of surgical anesthesia below the hip.

Patient Concerns: A 46-year-old man with tuberculosis, chronic obstructive pulmonary disease, pulmonary heart disease, World Health Organization (WHO) class III pulmonary hypertension and right heart dysfunction, and American Society of Anesthesiologists physical status class III needed fixation of an intramedullary nail in the left lower extremity. Additionally, he had broken his left lower limb after a recent fall. Both general anesthesia and epidural anesthesia were not appropriate.

Diagnoses: The patient had a clear history of tuberculosis, computerized tomography scan displayed destructive pneumonophthisis. Furthermore, he had chronic obstructive pulmonary disease and pulmonary heart disease.

Interventions: An ultrasound-guided SIFI combined with a sacral plexus block was successfully performed for surgical anesthesia and avoided all hemodynamic fluctuations.

Outcomes: We successfully performed an ultrasound-guided SIFI combined with a sacral plexus block for surgical anesthesia and avoided all hemodynamic fluctuations.

Lessons: Ultrasound-guided suprainguinal fascia iliaca block combined with a sacral plexus block can be suitable for anesthesia for patients with severe circulatory compromise.
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http://dx.doi.org/10.1097/MD.0000000000021921DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7458218PMC
August 2020

Nomogram for Predicting COVID-19 Disease Progression Based on Single-Center Data: Observational Study and Model Development.

JMIR Med Inform 2020 Sep 8;8(9):e19588. Epub 2020 Sep 8.

Renmin Hospital, Wuhan University, Wuhan, China.

Background: In late December 2019, a pneumonia caused by SARS-CoV-2 was first reported in Wuhan and spread worldwide rapidly. Currently, no specific medicine is available to treat infection with COVID-19.

Objective: The aims of this study were to summarize the epidemiological and clinical characteristics of 175 patients with SARS-CoV-2 infection who were hospitalized in Renmin Hospital of Wuhan University from January 1 to January 31, 2020, and to establish a tool to identify potential critical patients with COVID-19 and help clinical physicians prevent progression of this disease.

Methods: In this retrospective study, clinical characteristics of 175 confirmed COVID-19 cases were collected and analyzed. Univariate analysis and least absolute shrinkage and selection operator (LASSO) regression were used to select variables. Multivariate analysis was applied to identify independent risk factors in COVID-19 progression. We established a nomogram to evaluate the probability of progression of the condition of a patient with COVID-19 to severe within three weeks of disease onset. The nomogram was verified using calibration curves and receiver operating characteristic curves.

Results: A total of 18 variables were considered to be risk factors after the univariate regression analysis of the laboratory parameters (P<.05), and LASSO regression analysis screened out 10 risk factors for further study. The six independent risk factors revealed by multivariate Cox regression were age (OR 1.035, 95% CI 1.017-1.054; P<.001), CK level (OR 1.002, 95% CI 1.0003-1.0039; P=.02), CD4 count (OR 0.995, 95% CI 0.992-0.998; P=.002), CD8 % (OR 1.007, 95% CI 1.004-1.012, P<.001), CD8 count (OR 0.881, 95% CI 0.835-0.931; P<.001), and C3 count (OR 6.93, 95% CI 1.945-24.691; P=.003). The areas under the curve of the prediction model for 0.5-week, 1-week, 2-week and 3-week nonsevere probability were 0.721, 0.742, 0.87, and 0.832, respectively. The calibration curves showed that the model had good prediction ability within three weeks of disease onset.

Conclusions: This study presents a predictive nomogram of critical patients with COVID-19 based on LASSO and Cox regression analysis. Clinical use of the nomogram may enable timely detection of potential critical patients with COVID-19 and instruct clinicians to administer early intervention to these patients to prevent the disease from worsening.
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http://dx.doi.org/10.2196/19588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485996PMC
September 2020

CircC3P1 attenuated pro-inflammatory cytokine production and cell apoptosis in acute lung injury induced by sepsis through modulating miR-21.

J Cell Mol Med 2020 10 26;24(19):11221-11229. Epub 2020 Aug 26.

Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Acute lung injury (ALI) induced by sepsis is characterized by an inflammatory process related to the up-regulation of inflammatory cytokines and chemokines. In the present study, we explored the role of circC3P1 in sepsis-induced ALI in vitro and in vivo. The caecal ligation and puncture (CLP)-induced sepsis model was established through CLP surgery. Forty adult male C57BL/6 mice were randomly assigned into sham, CLP, CLP + vector and CLP + circC3P1 (each n = 10). Primary murine pulmonary microvascular endothelial cells (MPVECs) were transfected with circC3P1 or empty vector 24 hours prior to LPS treatment via Lipofectamine 2000. The expressions of circC3P1, tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β were evaluated after 6-h LPS treatment. Cell apoptosis was evaluated via flow cytometry. The CLP group demonstrated pulmonary morphological abnormalities, increased concentrations of TNF-α, IL-6 and IL-1β in the lung tissue, compared with the sham group. MPVECs treated with LPS significantly elevated TNF-α, IL-6 and IL-1β levels and increased cell apoptosis than that in the control group. The circC3P1 overexpression in sepsis-induced ALI mice attenuated pulmonary injury, inflammation and apoptosis. Besides, circC3P1 revealed anti-inflammatory and anti-apoptotic effect in MPVEC-treated LPS. CircC3P1 overexpression reduced cell apoptosis and pro-inflammatory cytokines levels via down-regulating miR-21. CircC3P1 attenuated pro-inflammatory cytokine production and cell apoptosis in ALI induced by sepsis through modulating miR-21, indicating that circC3P1 is a promising therapeutic biomarker for sepsis-induced ALI.
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http://dx.doi.org/10.1111/jcmm.15685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576301PMC
October 2020