Publications by authors named "Tao Chen"

3,238 Publications

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Vitiligo-Like Depigmentation Induced by Anti-Programmed Death 1 Antibody.

Dermatitis 2022 Aug 12. Epub 2022 Aug 12.

Department of Dermatovenereology, Chengdu Second People's Hospital, China

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http://dx.doi.org/10.1097/DER.0000000000000932DOI Listing
August 2022

Fine particulate matter induces heart defects via AHR/ROS-mediated endoplasmic reticulum stress.

Chemosphere 2022 Aug 7;307(Pt 2):135962. Epub 2022 Aug 7.

Suzhou Medical College of Soochow University, Suzhou, China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Soochow University, Suzhou, China. Electronic address:

Accumulating body of evidence indicates that exposure to fine particulate matter (PM) is closely associated with congenital heart disease in the offspring, but the underlying molecular mechanisms remain to be elucidated. We previously reported that extractable organic matter (EOM) from PM induces reactive oxygen species (ROS) overproduction by activating aromatic hydrocarbon receptor (AHR), leading to heart defects in zebrafish embryos. We hypothesized that endoplasmic reticulum (ER) stress might be elicited by the excessive ROS production and thereby contribute to the cardiac developmental toxicity of PM. In this study, we examined the effects of EOM on endoplasmic reticulum (ER) stress, apoptosis, and Wnt signal pathway in zebrafish embryos, and explored their roles in EOM-induced heart defects. Our results showed that 4-Phenylbutyric acid (4-PBA), a pharmaceutical inhibitor of ER stress, significantly attenuated the EOM-elevated heart malformation rates. Moreover, EOM upregulated the expression levels of ER stress marker genes including CHOP and PDI in the heart of zebrafish embryos, which were counteracted by genetic or pharmaceutical inhibition of AHR activity. The ROS scavenger N-Acetyl-l-cysteine (NAC) also abolished the EOM-induced ER stress. We further demonstrated that both 4-PBA and CHOP genetic knockdown rescued the PM-induced ROS overproduction, apoptosis and suppression of Wnt signaling. In conclusion, our results indicate that PM induces AHR/ROS-mediated ER stress, which leads to apoptosis and Wnt signaling inhibition, ultimately resulting in heart defects.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135962DOI Listing
August 2022

GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation.

Cell Discov 2022 Aug 9;8(1):77. Epub 2022 Aug 9.

State Key Laboratory of Respiratory Disease; National Clinical Research Center of Respiratory Disease; Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.

Reprogrammed cell metabolism is deemed as one of the hallmarks of cancer. Hexosamine biosynthesis pathway (HBP) acts as an "energy sensor" in cells to regulate metabolic fluxes. Glutamine-fructose-6-phosphate amidotransferase 1 (GFAT1), the rate-limiting enzyme of HBP, is broadly found with elevated expression in human cancers though its exact and concrete role in tumorigenesis still remains unknown and needs further investigation. P38 mitogen-activated protein kinase (MAPK) is an important component of stress-signaling pathway and plays a critical role in cell fate decision, whereas the underlying mechanism of its activation under nutrient stress also remains elusive. In this study, we show that glucose deprivation induces the interaction of GFAT1 with transforming growth factor β-activated kinase 1 binding protein 1 (TAB1) in a TAB1 S438 phosphorylation-dependent manner. Subsequently, the binding of GFAT1 to TAB1 facilitates TTLL5-GFAT1-TAB1 complex formation, and the metabolic activity of GFAT1 for glutamate production further contributes to TTLL5-mediated TAB1 glutamylation. In consequence, TAB1 glutamylation promotes the recruitment of p38α MAPK and thus drives p38 MAPK activation. Physiologically, GFAT1-TAB1-p38 signaling promotes autophagy occurrence and thus protects tumor cell survival under glucose deficiency. Clinical analysis indicates that both GFAT1 and TAB1 S438 phosphorylation levels correlate with the poor prognosis of lung adenocarcinoma patients. These findings altogether uncover an unidentified mechanism underlying p38 MAPK signaling regulation by metabolic enzyme upon nutrient stress and provide theoretical rationality of targeting GFAT1 for cancer treatment.
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http://dx.doi.org/10.1038/s41421-022-00423-0DOI Listing
August 2022

Cooperative Coupling of H O Production and Organic Synthesis Over Floatable Polystyrene Sphere-supported TiO /Bi O S-scheme Photocatalyst.

Adv Mater 2022 Aug 9:e2203225. Epub 2022 Aug 9.

Laboratory of Solar Fuel, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, 430074, P. R. China.

Cooperative coupling of photocatalytic H O production with organic synthesis has an expansive perspective in converting solar energy into storable chemical energy. However, traditional powder photocatalysts suffer from severe agglomeration, limited light absorption, poor gas reactant accessibility and reusable difficulty, which greatly hinders their large-scale application. Herein, floatable composite photocatalysts are synthesized by immobilizing hydrophobic TiO and Bi O on light-weight polystyrene (PS) spheres via hydrothermal and photodeposition methods. The floatable photocatalysts are not only solar transparent, but also upgrade the contact between reactants and photocatalysts. Thus, the floatable step-scheme (S-scheme) TiO /Bi O photocatalyst exhibits a drastically enhanced H O yield of 1.15 mM h and decent furfuryl alcohol conversion to furoic acid synchronously. Furthermore, the S-scheme mechanism and dynamics are systematically investigated by in-situ irradiated X-ray photoelectron spectroscopy and femtosecond transient absorption spectrum analyses. In-situ Fourier transform infrared spectroscopy and density functional theory (DFT) calculations reveal the mechanism of furoic acid evolution. The ingenious design of floatable photocatalysts not only furnishes insight into maximizing photocatalytic reaction kinetics but also provides a new route for high-efficient heterogeneous catalysis. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202203225DOI Listing
August 2022

A new method to select frequency band for vibration signal demodulation and condition estimation of rolling bearings.

ISA Trans 2022 Jul 20. Epub 2022 Jul 20.

CRRC Qingdao Sifang Rolling Stock Research Institute Co., LTD, Qingdao 266031, China.

The narrowband amplitude demodulation of vibration signals is widely used to extract components carrying information about rolling bearing faults for condition estimation. However, precisely selecting the frequency band determines the quality of demodulation. Although transient detection based methods have achieved satisfactory performance in some scenarios, they may be severely disturbed by strong random impulses or harmonics. Additionally, frequency division strategy is always considered as rough. Aiming at these problems, a new method is proposed to select the optimal frequency band by combining the frequency division strategy, genetic algorithm (GA) optimization, and an automatic fault characteristic order (FCO) search algorithm. Aimed at a signal, its sub-signals are extracted according to the frequency division strategy and the order tracing technology is implemented to obtain the order spectrum of each sub-signal at first. Then, the FCO and its multiples (FCOs) are automatically searched from each order spectrum, and their amplitude summation is calculated. Afterwards, the ratio of FCOs summation to noise amplitude (RFN) is measured as the basis to construct an RFNgram for selecting the primary optimal frequency band Finally, GA is implemented to select the optimized optimal frequency band where the signal is demodulated for estimating the bearing condition. RFN measures both the impulsiveness and periodicity of signals, and GA optimizes the result from a RFNgram, essentially making up for the shortcomings of previous methods under the condition of multiple interferences. RFN is measured through the order spectrum, which also allows it to be applicable to varying speeds, thus achieving a more comprehensive range of industrial applications. Three case studies are implemented to present the superiority of the proposed method in condition estimation for rolling bearings by comparison with four classical or advanced methods.
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http://dx.doi.org/10.1016/j.isatra.2022.07.019DOI Listing
July 2022

Distribution and driving factors of antibiotic resistance genes in treated wastewater from different types of livestock farms.

Sci Total Environ 2022 Aug 4:157837. Epub 2022 Aug 4.

Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China; Ministry of Agriculture Key Laboratory of Tropical Agricultural Environment, South China Agricultural University, Guangzhou 510642, China; Guangdong Engineering Technology Research Center of Harmless Treatment and Resource Utilization of Livestock Waste, Yunfu, Xinxing 527400, China. Electronic address:

Treated wastewater from livestock farms is an important reservoir for antibiotic resistance genes (ARGs), and is a main source of ARGs in the environment. However, the distribution and driving factors of ARGs in treated wastewater from different types of livestock farms are rarely reported. In this study, treated wastewater from 69 large-scale livestock farms of different types, including broiler, layer, and pig farms, was collected, and 11 subtypes of ARGs, 2 mobile genetic elements (MGEs) and bacterial community structure were analyzed. The results revealed detection rates of NDM-1 and mcr-1 of 90 % and 43 %, respectively, and the detection rates of other ARGs were 100 %. The relative abundance of ARGs, such as tetA, tetX and strB, in broiler farms was significantly higher than that in layer farms, but the bacterial α diversity was significantly lower than that in other farm types. Furthermore, although the treatment process had a greater impact on the physicochemical properties of the treated wastewater than the livestock type, livestock type was the main factor affecting the bacterial community in the treated wastewater. The analysis of potential host bacteria of ARGs revealed significant differences in the host bacteria of ARGs in treated wastewater from different types of livestock farms. The host bacteria of ARGs in broiler farms mainly belonged to Actinobacteria, layer farms mainly belonged to Proteobacteria, and pig farms mainly belonged to Firmicutes. Additionally, redundancy analysis showed that the distribution of ARGs may have resulted from the combination of multiple driving factors in different types of livestock farms, among which tnpA and NH-N were the main influencing factors. This study revealed multiple driving factors for the distribution of typical ARGs in treated wastewater from different types of livestock farms, providing basic data for the prevention and control of ARG pollution in agricultural environments.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157837DOI Listing
August 2022

Incidence and economic burden of herpes zoster and its complications in Chinese immunocompromised adults.

J Infect 2022 Aug 3. Epub 2022 Aug 3.

The Institute of Infectious Disease and Vaccine, School of Public Health. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2022.07.028DOI Listing
August 2022

Outcome of a novel self-control stricture-preventing water balloon for complete circular esophageal endoscopic submucosal dissection.

Surg Endosc 2022 Aug 5. Epub 2022 Aug 5.

Endoscopy Center, Department of Gastroenterology, Shanghai East Hospital, School of Medicine, Tongji University, 150 Jimo Road, Shanghai, 200120, China.

Objectives: Post-ESD esophageal stricture especially after wholly circumferential ESD remains an unresolved issue without ideal strategies. Our initiative novel self-control stricture-preventing water balloon may be an alternative.

Methods: Patients with esophageal neoplastic lesions expected to result in a whole circular mucosa defect after esophageal ESD from February 2018 to August 2020 were included in the study. We used a novel self-control stricture-preventing water balloon combined with oral prednisolone as preventive strategy for the enrolled patients.

Results: Thirty-seven patients (9 females and 28 males, patients aged 52 to 82 years) finished the 12-week treatment including steroid treatment and balloon placement. The median size of longitudinal diameter was 7 cm (range from 4 to 14 cm). All the lesions achieved curative resection and the median procedure time was 110 min (range 50 to 180 min). Balloons were found migration in 4 patients. As a result, there were 3 patients (8.1%) experienced stricture. Generally, patients could tolerate to balloons, only with mild uncomfortableness, such as occasional sore throat, cough, and retrosternal pain. In addition, during the follow-up period, no significant adverse events associated to oral steroid administration were observed and no recurrence was found.

Conclusions: Our novel self-control stricture-preventing water balloon based on the oral steroid therapy is effective and safe. This strategy well prevents esophageal stricture after complete circumferential ESD.
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http://dx.doi.org/10.1007/s00464-022-09456-8DOI Listing
August 2022

Predictive Value of the Log Odds of Negative Lymph Nodes/T Stage as a Novel Prognostic Factor in Bladder Cancer Patients After Radical Cystectomy.

Front Oncol 2022 19;12:895413. Epub 2022 Jul 19.

Department of Urology, the First Affiliated Hospital of Nanchang University, Nanchang City, China.

Background: The effect of lymph node resection on the prognosis of bladder cancer (BLCA) patients receiving radical cystectomy should not be ignored. Our aim was to explore the prognostic value of the log odds of negative lymph nodes/T stage (LONT) and construct a more effective nomogram based on LONT to predict cancer-specific survival (CSS) in postoperative BLCA patients.

Methods: Patients diagnosed with BLCA after radical cystectomy between 2004 and 2015 in the Surveillance, Epidemiology, and End Results (SEER) database were enrolled. We randomly split (7:3) these patients into the primary cohort and internal validation cohort. 86 patients from the First Affiliated Hospital of Nanchang University were collected as the external validation set. Univariate and multivariate cox regression analyses were carried out to seek prognostic factors of postoperative BLCA patients. According to these significantly prognostic factors, a simple-to-use nomogram was established for predicting CSS. Their performances were evaluated by using calibration curves, the concordance index (C-index), the receiver operating characteristic (ROC) curves, and decision curve analysis (DCA). In addition, different risk groups were tested by Kaplan-Meier curves and log-rank tests.

Result: Whether in cancer-specific survival (CSS) or overall survival (OS), LONT was an independent and significant prognostic factor. Through further screening, the ultimate nomogram of CSS was composed of nine independent prognostic factors including LONT, age, race, tumor size, histologic type, T stage, N stage, summary stage and chemotherapy. The C-index of nomogram in the primary cohort, internal and external validation cohort were 0.734, 0.720 and 0.728, respectively. The AUC of predicting CSS at 3 and 5 years were 0.783 and 0.774 in the primary cohort and 0.781 and 0.781 in the validation cohort. The results of calibration and DCA showed good concordance and clinical applicability. Significant differences (P < 0.05) were displayed in CSS among different risk groups.

Conclusion: LONT was regarded as a novel and reliable prognostic factor. Compared with the AJCC staging system, the established nomogram based on LONT can more effectively predict the prognosis of BLCA patients after radical cystectomy.
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http://dx.doi.org/10.3389/fonc.2022.895413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343753PMC
July 2022

FGL2-MCOLN3-autophagy axis-triggered neutrophil extracellular traps exacerbate liver injury in fulminant viral hepatitis.

Cell Mol Gastroenterol Hepatol 2022 Aug 1. Epub 2022 Aug 1.

Department and institute of infectious diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China; National Medical Center for Major Public Health Events, Wuhan, China; State Key Laboratory for Zoonotic Diseases. Electronic address:

Background & Aims: Fulminant viral hepatitis (FVH) is a life-threatening disease, but its pathogenesis is not fully understood. Neutrophil extracellular traps (NETs) were an unrecognized link between inflammation and coagulation, which are two main features of FVH. Here, we aim to investigate the role and mechanism of NETs in the pathogenesis of FVH.

Methods: A mice model of FVH was established by murine hepatitis virus strain-3 (MHV-3) infection. Liver leukocytes of infected or uninfected mice were used for single cell RNA sequencing and whole transcriptome sequencing. NETs depletion was achieved using DNase1. Acetaminophen was used to establish a mice model of non-virus caused acute liver failure. Clinically, NETs-related markers in liver, plasma and peripheral neutrophils were assessed in patients with HBV-related acute liver injury (HBV-ALI).

Results: Increased hepatic NETs formation was observed in MHV-3-infected mice but not in acetaminophen-treated mice. NETs depletion improved the liver damage and survival rate in FVH by inhibiting hepatic fibrin deposition and inflammation. Adoptive transfer experiment showed that neutrophil-specific FGL2 promoted NETs formation. FGL2 was found to directly interact with mucolipin3 (MCOLN3), which regulated calcium influx and initiated autophagy, leading to NETs formation. Clinically, elevated plasma NETs level was associated with coagulation dysfunction in patients with HBV-ALI. Colocalization of FGL2, NETs and fibrin in liver was observed in these patients.

Conclusion: NETs aggravated liver injury in FVH by promoting fibrin deposition and inflammation. NETs formation was regulated by the FGL2-MCOLN3-autophagy axis. Targeting NETs may provide a new strategy for the treatment of FVH.
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http://dx.doi.org/10.1016/j.jcmgh.2022.07.014DOI Listing
August 2022

A Questionnaire-Based Ensemble Learning Model to Predict the Diagnosis of Vertigo: Model Development and Validation Study.

J Med Internet Res 2022 Aug 3;24(8):e34126. Epub 2022 Aug 3.

Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China.

Background: Questionnaires have been used in the past 2 decades to predict the diagnosis of vertigo and assist clinical decision-making. A questionnaire-based machine learning model is expected to improve the efficiency of diagnosis of vestibular disorders.

Objective: This study aims to develop and validate a questionnaire-based machine learning model that predicts the diagnosis of vertigo.

Methods: In this multicenter prospective study, patients presenting with vertigo entered a consecutive cohort at their first visit to the ENT and vertigo clinics of 7 tertiary referral centers from August 2019 to March 2021, with a follow-up period of 2 months. All participants completed a diagnostic questionnaire after eligibility screening. Patients who received only 1 final diagnosis by their treating specialists for their primary complaint were included in model development and validation. The data of patients enrolled before February 1, 2021 were used for modeling and cross-validation, while patients enrolled afterward entered external validation.

Results: A total of 1693 patients were enrolled, with a response rate of 96.2% (1693/1760). The median age was 51 (IQR 38-61) years, with 991 (58.5%) females; 1041 (61.5%) patients received the final diagnosis during the study period. Among them, 928 (54.8%) patients were included in model development and validation, and 113 (6.7%) patients who enrolled later were used as a test set for external validation. They were classified into 5 diagnostic categories. We compared 9 candidate machine learning methods, and the recalibrated model of light gradient boosting machine achieved the best performance, with an area under the curve of 0.937 (95% CI 0.917-0.962) in cross-validation and 0.954 (95% CI 0.944-0.967) in external validation.

Conclusions: The questionnaire-based light gradient boosting machine was able to predict common vestibular disorders and assist decision-making in ENT and vertigo clinics. Further studies with a larger sample size and the participation of neurologists will help assess the generalization and robustness of this machine learning method.
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http://dx.doi.org/10.2196/34126DOI Listing
August 2022

Predictive ability of the total score of the Kihon checklist for the incidence of functional disability in older Japanese adults: An 8-year prospective study.

Geriatr Gerontol Int 2022 Aug 2. Epub 2022 Aug 2.

Center for Liberal Arts, Fukuoka Institute of Technology, Fukuoka, Japan.

Aim: To investigate the association between the total score of the Kihon checklist (t-KCL score) and functional disability over an 8-year follow-up period, and to examine whether the t-KCL score in the basic model with risk factors contributes to the incremental predictive ability for functional disability among older adults.

Methods: We followed 2209 older adults aged ≥65 years without functional disability at baseline. The t-KCL score was determined using a baseline survey questionnaire. Functional disability was defined based on information from long-term care certifications. The association between the t-KCL score and functional disability was examined using the Cox proportional hazards model. The incremental predictive ability of the t-KCL score for functional disability was evaluated by the difference of the C-statistic, category-free net reclassification improvement (NRI), and integrated discrimination improvement (IDI).

Results: The median follow-up period was 7.8 years, and 557 participants developed functional disability. The adjusted hazard ratio (95% confidence interval [CI]) of functional disability for a 1-point increase of the t-KCL score was 1.08 (1.06-1.10). Adding the t-KCL score to the basic model significantly improved the C-statistic (95% CI) from 0.747 (0.728-0.768) to 0.760 (0.741-0.781). When the t-KCL score was added to the basic model, the NRI and IDI were 0.187 (95% CI: 0.095-0.287) and 0.020 (95% CI: 0.012-0.027), respectively.

Conclusions: The t-KCL score had an independent positive association with functional disability over an 8-year follow-up. Furthermore, adding the t-KCL score to the basic model improved the predictive ability for functional disability. Geriatr Gerontol Int 2022; ••: ••-••.
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http://dx.doi.org/10.1111/ggi.14435DOI Listing
August 2022

Occult suprachoroidal foreign bodies: a 3-case report.

Int J Ophthalmol 2022 18;15(7):1217-1220. Epub 2022 Jul 18.

Department of Ophthalmology, Shaanxi Eye Hospital, Xi'an People's Hospital (Xi'an Fourth Hospital), Affiliated Guangren Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.

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http://dx.doi.org/10.18240/ijo.2022.07.27DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318082PMC
July 2022

MXene-Derived 3D Defect-Rich [email protected] Graphene Oxide Aerogel with Ultrafast Carrier Separation for Photo-Assisted Uranium Extraction: A Combined Batch, X-ray Absorption Spectroscopy, and Density Functional Theory Calculations.

Inorg Chem 2022 Aug 1. Epub 2022 Aug 1.

State Key Laboratory of Environmentally Friendly Energy Materials, National Collaborative Innovation Center for Nuclear Waste and Environmental Safety, School of National Defence, Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Sichuan Civil-military Integration Institute, Southwest University of Science and Technology, Mianyang 621010, China.

Encapsulation of nano-semiconductor materials in three-dimensional (3D) adsorbents to build a typical semiconductor-adsorbent heterostructure is a forward-looking strategy for photo-assisted uranium extraction. Here, we develop 3D MXene-derived TiO(M)@reduced graphene oxide (RGO) aerogel for photo-assisted uranium extraction. Theoretical simulations demonstrate that oxygen vacancies on TiO(M) tailor the energy level structure and enhance the electron accumulation at gap states of TiO(M), thereby further realizing the spatial separation efficiency of electron-hole pairs by the Schottky junction. By virtue of the in situ X-ray photoelectron spectroscopy spectrum, we identify that photogenerated electrons generated over TiO(M) were transferred to graphene oxide aerogel by the Schottky junction. Accordingly, TiO (M)@RGO aerogel presents a considerable removal efficiency for U(VI) with a removal ratio of 95.7%. Relying on the X-ray absorption spectroscopy technique, we distinguish the evolution of 2HO-2O-U-5O into HO-2O-U-3O from dark to light conditions, further confirming the reduction of high-valent uranium. This strategy may open a paradigm for developing novel heterojunctions as photocatalysts for selective U(VI) extraction.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01850DOI Listing
August 2022

Analytical techniques for characterizing diastereomers of phosphorothioated oligonucleotides.

J Chromatogr A 2022 Jul 16;1678:463349. Epub 2022 Jul 16.

Small Molecule Analytical Chemistry, Small Molecule Pharmaceutical Sciences, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, United States. Electronic address:

Oligonucleotides have emerged as powerful therapeutics for treating diverse diseases. To fully unlock the therapeutic potential of oligonucleotides, there is still a great need to further improve their drug-like properties. Numerous chemical modifications have been explored to achieve this goal, with phosphorothioation being one of the most widely used strategies. However, phosphorothioate modification produces diastereomers that are reported to have different properties and performances, demanding detailed characterization of these diastereomers. Here we provide an overview of phosphorothioated oligonucleotide diastereomers, covering their origin and configurations, physicochemical and pharmacological properties, and stereo-selective chemical synthesis, followed by a summary of currently available analytical techniques for characterizing these diastereomers, with a focus on liquid chromatography-based approaches, including ion-pair reversed-phase liquid chromatography, anion exchange chromatography, mixed-mode chromatography, and hybrid approaches. Non-chromatographic techniques, such as capillary electrophoresis, spectroscopy and other methods, are also being reviewed.
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http://dx.doi.org/10.1016/j.chroma.2022.463349DOI Listing
July 2022

Mass Transfer Printing of Metal-Halide Perovskite Films and Nanostructures.

Adv Mater 2022 Jul 30:e2203529. Epub 2022 Jul 30.

CAS Key Laboratory of Strongly-coupled Quantum Matter Physics, Department of Physics, University of Science and Technology of China, Hefei, Anhui, 230026, China.

Most methods of depositing perovskite films cannot meet the diverse requirements of real applications such as depositing films on various types of substrates, making patterns with different bandgaps for full-color display. Here, a robust mass transfer method of perovskite films and nanostructures is reported, meeting those requirements, by using an ultrathin branched polyethylenimine as interfacial chemical bonding layers. The transfer-printed perovskite films exhibit comparable morphology, composition, optoelectronic properties, and device performances with the counterparts made by optimized spin-coating methods. The perovskite light-emitting diodes (PeLEDs) using the transfer-printed films show decent external quantum efficiencies of 10.5% and 6.7% for red (680 nm) and sky-blue (493 nm) emissions, which are similar to the devices made by spin-coating. This robust transfer printing method also enables the the preparation of perovskite micropatterns with a high resolution up to 1270 pixels per inch. Horizontally aligned red and sky-blue perovskite microstripes are further obtained through multiple printing processes for white PeLEDs. This work demonstrates a feasible strategy for making perovskite films or micropatterns on various substrates for real applications in full-color display, white LEDs, lasing, etc.
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http://dx.doi.org/10.1002/adma.202203529DOI Listing
July 2022

72 weeks post-partum follow-up of dolutegravir versus efavirenz initiated in late pregnancy (DolPHIN-2): an open-label, randomised controlled study.

Lancet HIV 2022 08;9(8):e534-e543

Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, UK; Tropical Infectious Diseases Unit, Liverpool University Hospitals NHS Foundation Trust, Liverpool, UK. Electronic address:

Background: Late initiation of antiretrovirals in pregnancy is associated with increased risk of perinatal transmission and higher infant mortality. We report the final 72-week postpartum results for efficacy and safety of dolutegravir-based compared with efavirenz-based regimens in mothers and infants.

Methods: DolPHIN-2 was a randomised, open-label trial. Pregnant women in South Africa and Uganda aged at least 18 years, with untreated but confirmed HIV infection and an estimated gestation of at least 28 weeks, initiating antiretroviral therapy in third trimester were eligible for inclusion. Eligible women were randomly assigned (1:1) to receive either dolutegravir-based (50 mg dolutegravir, 300 mg tenofovir disoproxil fumarate, and either 200 mg emtricitabine in South Africa or 300 mg lamivudine in Uganda) or efavirenz-based (fixed dose combination 600 mg tenofovir disoproxil fumarate plus either emtricitabine in South Africa or lamivudine in Uganda) therapy. The primary efficacy outcome was the time to a viral load of less than 50 copies per mL measured at 6, 12, 24, 48, and 72 weeks postpartum with a Cox model adjusting for viral load and CD4 cell count. Safety endpoints were summarised by the number of women and infants with events. This trial is registered with ClinicalTrials.gov, NCT03249181.

Findings: Between Jan 23 and Aug 15, 2018, 280 women were screened for inclusion, of whom 268 (96%) women were randomly assigned: 133 (50%) to the efavirenz group and 135 (50%) to the dolutegravir group. 250 (93%; 125 [50%] in the efavirenz group and 125 [50%] in the dolutegravir group) women were included in the intention-to-treat analysis of efficacy. Median time to viral load of less than 50 copies per mL was 4·1 weeks (IQR 4·0-5·1) in the dolutegravir group compared with 12·1 weeks (10·7-13·3) in the efavirenz group (adjusted hazard ratio [HR] 1·93 [95% CI 1·5-2·5]). At 72 weeks postpartum, 116 (93%) mothers in the dolutegravir group and 114 (91%) in the efavirenz group had a viral load of less than 50 copies per mL. Of 57 (21%) mothers with a severe adverse event, three (2%) in the dolutegravir group and five (4%) in the efavirenz group were related to the drug (dolutegravir drug-related events were one woman each with suicidal ideation, suicide attempt, herpes zoster meningitis; efavirenz drug-related events were one woman each with suicide attempt and liver cirrhosis, and three people with drug-induced liver injury). Of 136 (56%) infants in whom severe adverse events were recorded, none were related to the study drugs. In addition to the three infant HIV infections detected at birth in the dolutegravir group that have been previously reported, an additional transmission in the efavirenz group occurred during breastfeeding despite optimal maternal viral suppression and serial negative infant tests in the first year of life.

Interpretation: Dolutegravir was safe and well tolerated, supporting updated WHO treatment recommendations in pregnant and breastfeeding women. Infant HIV transmissions can occur during breastfeeding despite persistently undetectable maternal viral load highlighting the need for continued infant testing.

Funding: Unitaid.
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http://dx.doi.org/10.1016/S2352-3018(22)00173-4DOI Listing
August 2022

Knockdown of hsa_circ_0005699 attenuates inflammation and apoptosis induced by ox-LDL in human umbilical vein endothelial cells through regulation of the miR-450b-5p/NFKB1 axis.

Mol Med Rep 2022 Sep 29;26(3). Epub 2022 Jul 29.

Department of Vascular Surgery, Ganzhou People's Hospital, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, Jiangxi 341000, P.R. China.

Atherosclerosis (AS) remains the leading cause of mortality throughout the world, and vascular endothelial cell dysfunction is one of the key events leading to this pathology. In recent years, there has been an increased interest in the role of circulating RNAs in various diseases; these noncoding RNAs can regulate gene products by acting as microRNA (miR) sponges. Furthermore, it has been shown that foam cells exhibit high expression levels of hsa_circ_0005699 (circ_0005699); however, to the best of our knowledge, no previous study has investigated the role of circ_0005699 in the regulation of vascular endothelial function. The present study employed human umbilical vein endothelial cells (HUVECs), which have been widely used to study vascular endothelial cell function. In addition, apolipoprotein E (ApoE)-deficient mice were used, which have been shown to rapidly develop AS and are widely used as a model of this disease. Cellular and biochemical techniques were performed, including gene transfection and short hairpin RNA-mediated gene silencing for cell transfection, luciferase reporter gene assay to confirm predicted genes, Cell Counting Kit-8 assay and flow cytometry to assess cell viability and apoptosis, and reverse transcription-quantitative PCR and western blotting for detection of mRNA and protein expression. In the present study, the expression levels of circ_0005699 were increased by oxidized low-density lipoprotein in a time- and dose-dependent manner in HUVECs; this was also associated with increased apoptosis of these cells. In addition, the expression levels of circ_0005699 were elevated, along with increased levels of inflammatory cytokines, in ApoE-deficient mice. An RNA pull-down assay indicated that circ_0005699 can bind miR-450b-5p to decrease its expression, whereas silencing of circ_0005699 resulted in increased expression of miR-450b-5p. In addition, the online bioinformatics tool starBase predicted NFKB1 as a target gene of miR-450b-5p, which was further confirmed by the luciferase reporter gene assay. Notably, knockdown of circ_0005699 resulted in the increased survival of HUVECs, which was associated with decreased protein expression levels of NFKB1 and inflammatory cytokines. By contrast, the effects of circ-0005699 silencing on survival were reversed by miR-450b-5p inhibition or NFKB1 overexpression. In conclusion, knockdown of circ_0005699 may ameliorate endothelial cell injury through regulation of the miR-450b-5P/NFKB1 signaling axis.
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http://dx.doi.org/10.3892/mmr.2022.12806DOI Listing
September 2022

A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant .

Front Microbiol 2022 12;13:956044. Epub 2022 Jul 12.

Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Ceftazidime/avibactam (CZA) is an alternative antibiotic used for the treatment of infections caused by carbapenem-resistant (CRE). However, the CZA-resistant CRE strains have been detected worldwide. Therefore, it is critical to screen CZA-resistant CRE strains in colonized patients or a specific population so as to rapidly implement infection control measures to limit their transmission. In this study, we developed a - (SS) CZA-selective medium and assessed its performance to screen for clinical CZA-resistant CRE isolates in both pure-strain specimens and stool samples. A total of 150 non-duplicated isolates, including 75 CZA-susceptible and 75 CZA-resistant CRE pathogens, were tested by using the broth microdilution method and the SS CZA medium, respectively. The bacterial suspensions were serially diluted in the SS CZA medium, which showed excellent screening performance in both pure CZA-resistant CRE strain and the stool samples with the lowest detection limit of 10-10 and 10-10 CFU/ml, respectively. Notably, none of the susceptible isolates showed growth even at the highest dilution concentration of 10 CFU/ml. Most importantly, the SS CZA medium demonstrated excellent performance in screening simulated clinical polymicrobial specimens. Moreover, its screening performance was unaffected by the different resistance determinants for tested isolates. Cumulatively, our data suggest that the SS CZA medium can be used as a promising selective medium to screen CZA-resistant CRE, irrespective of their resistance mechanisms.
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http://dx.doi.org/10.3389/fmicb.2022.956044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315207PMC
July 2022

Mimicking Color-Changing Organisms to Enable the Multicolors and Multifunctions of Smart Fluorescent Polymeric Hydrogels.

Acc Chem Res 2022 Jul 28. Epub 2022 Jul 28.

Key Laboratory of Marine Materials and Related Technologies, Chinese Academy of Sciences, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, 1219 Zhongguan West Road, Ningbo 315201, China.

ConspectusFluorescent polymer hydrogels (FPHs) are of significant interest for diverse emerging applications such as visualized sensing, smart display, camouflaging skins, soft actuators/robots, because they can synergize the features of classic fluorescent polymers and hydrogels. With great efforts in the past decades, the major challenge in this field has been believed to be not whether a given FPH of interest can be prepared but how to fabricate robust FPHs with multicolor tunability and multifunctional synergy. Such materials will conceptually minimize the contribution of passive materials to the mass and size of the final system, holding great potential to facilitate multiple applications. To this end, one promising way is to learn from the Nature that has superb capability to forge delicate or sometimes beyond-imagination materials. Chameleons and cephalopods serve as typical examples, which are famous for not only diverse skin color adaptability under changing environmental demands, but also synergistic skin color and body gesture changes to communicate, warn, camouflage, etc. Biological studies revealed their structural color-changing capacity derives from different types of skin chromatophores and their rational multilayer arrangement in under-skin tissues. Besides, their superb ability to heterogeneously integrate soft tissues with disparate functions into topology-optimized architectures has led to various multifunctional performances. Such natural strategies, if replicated and implemented in artificial systems, would significantly benefit and advance the development of robust FPHs for various applications.In this Account, we summarizes the key advances of smart FPHs mainly achieved by our groups. We start by introducing the unique hierarchical multilayer structures of skin chromatophores in structural color-changing reptiles, followed by an in-depth discussion on how a rational integration of bioinspiration and man-made design makes it possible to largely expand the fluorescence color-changing range of smart FPHs to almost cover the whole visible spectrum. Then, to closely mimic the multifunctional behaviors of chameleons and cephalopods, we further develop efficient strategies to introduce supramolecular interactions or heterogeneously integrating smart FPHs with other soft materials with disparate functions, producing a number of multifunctional fluorescent polymeric hydrogel systems. These robust FPHs can find many frontier applications, including bioinspired synergistic color/shape switchable hydrogel actuators/robots, smart systems with on-demand fluorescent patterning capacities for displaying or information encryption, as well as robust chemosensors for important food or environmental analytes. We expect that the discussion presented in this Account would promote better understanding of the discoloration systems in nature, and advance the development of bioinspired color-changing materials.
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http://dx.doi.org/10.1021/acs.accounts.2c00320DOI Listing
July 2022

Application of Hz36 and Hk37 as Biocontrol Agents against Clubroot Caused by .

J Fungi (Basel) 2022 Jul 26;8(8). Epub 2022 Jul 26.

State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Clubroot, a soil-infective disease caused by , is a serious disease affecting cruciferous plants around the world. There is no effective control measure to completely remove this pathogen from fields after infection. Here, we screened and identified two strains (Hz36, ; Hk37, ) of from the gall of clubroot in rapeseed fields with biocontrol potential for clubroot. The fermentation broth of Hz36 could significantly inhibit the germination of resting spores of , and promote the seed germination and root growth of rapeseed. The biocontrol efficiency of Hz36 strain on clubroot for rapeseed and &nbsp; was 44.29% and 52.18%, respectively. The qPCR results revealed that strain Hz36 treatment could significantly reduce the content of in root cells, and paraffin section analysis revealed that it could delay the development of . Strain Hk37 showed similar effects to strain Hz36, whose biocontrol efficiency of clubroot could reach 57.30% in rapeseed and 68.01% in . . These results indicate that strains Hz36 and Hk37 have the potential for the biocontrol of clubroot.
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http://dx.doi.org/10.3390/jof8080777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331738PMC
July 2022

Activation of Persulfate for Degrading Tetracycline Using the Leaching Residues of the Lead-Zinc Flotation Tailing.

Polymers (Basel) 2022 Jul 21;14(14). Epub 2022 Jul 21.

School of Environment, South China Normal University, University Town, Guangzhou 510006, China.

Inappropriate disposal of leaching residues from the lead-zinc tailings recovery process may result in environmental pollution. Its recycling and reuse remain a prevalent topic in environmental science and technology. It was roasted to prepare leaching residues-based materials (TLRS) in this work, and the TLRS were creatively used as the catalyst to active sodium persulfate (PS) to degrade organic pollutants. Degradation of tetracycline using the TLRS-PS system was evaluated, and the treating parameters were optimized. Roasting resulted in the exposure of active sites on TLRS surface, in which transition metals can donate electrons to PS to form SO. SO can further react with OH to form ·OH. Formation of these radicals was confirmed by both quenching experiments and EPR analysis. Under optimized conditions, 85% of the TC can be degraded in 3.0 h, and ~50% of degraded TC was mineralized to CO and HO. The performance of TLRS barely changed after four reuses, suggesting the chemical stability of TLRS. The presence of dissolved substance in the water matrix could weaken the performance of the TLRS-PS system. A mechanism of TC degradation was proposed based on the experimental results and literature. These preliminary results provide us new insight on the reuse of lead-zinc flotation tailings.
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http://dx.doi.org/10.3390/polym14142959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316694PMC
July 2022

Efficient Removal of Methylene Blue by Bio-Based Sodium Alginate/Lignin Composite Hydrogel Beads.

Polymers (Basel) 2022 Jul 19;14(14). Epub 2022 Jul 19.

Hubei Key Laboratory of Radiation Chemistry and Functional Materials, Non-Power Nuclear Technology, Collaborative Innovation Center, Hubei University of Science and Technology, Xianning 437100, China.

Dye pollution is a serious issue in current environment protection, and bio-based adsorbents have been receiving much attention in wastewater treatment, due to their low cost, renewable, and environmentally friendly characteristics. Bio-based sodium alginate/lignin composite (SA/Lig) hydrogel beads were fabricated by a facile cross-linking with calcium ion and used for the removal of methylene blue (MB). The obtained SA/Lig microbeads were characterized with SEM, FTIR, and TG, and the effect of lignin content, pH, and temperature on the MB adsorption was investigated. The results indicated that the introduction of aromatic lignin can not only enhance thermal stability but also can improve the adsorption performance. Under optimal conditions, the maximum adsorption capacity (254.3 mg/g) was obtained for the SA/Lig-20% beads, with a removal efficiency of 84.8%. The adsorption process for MB is endothermic, and the rate-limiting step is chemical adsorption. The removal efficiency is higher than 90% after five cycles, revealing that the prepared beads show good regeneration ability.
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http://dx.doi.org/10.3390/polym14142917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320731PMC
July 2022

Influence of Pulse Energy and Defocus Amount on the Mechanism and Surface Characteristics of Femtosecond Laser Polishing of SiC Ceramics.

Micromachines (Basel) 2022 Jul 15;13(7). Epub 2022 Jul 15.

School of Mechanical and Electrical Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, China.

SiC ceramics have excellent comprehensive properties and are typical hard and brittle materials that are difficult to process and are widely used in many fields. Laser polishing technology has developed into a new surface processing technology, and femtosecond laser polishing has become an important method for the precision machining of hard and brittle materials. In this paper, SiC ceramics were ablated and polished by infrared femtosecond laser, the laser ablation threshold of SiC ceramics was calculated and the influence of pulse energy and defocus amount on the surface morphology, surface roughness, polishing depth and oxidation degree of femtosecond laser polishing of SiC ceramics were investigated. The results show that when the laser repetition frequency = 175 kHz, wavelength = 1064 nm and ablation time = 9 s, the laser ablation threshold of SiC ceramics is 0.355 J/cm. With the increase in pulse energy, the surface roughness first decreased and then increased, and the polishing depth showed an overall upward trend. The change of defocus amount will lead to the change of the laser spot diameter. With the increase of the defocus amount, the laser spot irradiated on the workpiece surface becomes larger, and the laser energy density decreases, which results in the decrease of the laser ablation ability and polishing depth and the increase of the polished surface roughness. Periodic nano-ripple structures appeared on the laser-induced surface. Through Energy Dispersive Spectrometer (EDS) elemental analysis, it was found that there was an oxidation phenomenon in SiC ceramics polished by femtosecond laser in an air environment, and the change of pulse energy and defocus amount had insignificant effects on the degree of oxidation.
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http://dx.doi.org/10.3390/mi13071118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324907PMC
July 2022

Patent bibliometric analysis for global trend of organoid technologies in the past decade.

iScience 2022 Aug 8;25(8):104728. Epub 2022 Jul 8.

Tissue Engineering and Organ Manufacturing (TEOM) Lab, Department of Biomedical Engineering, Wuhan University TaiKang Medical School (School of Basic Medical Sciences), Hubei 430071, China.

Organoids are considered a game-changing paradigm of research models for human physiology and disease, which provides unsurpassed opportunities across disciplines in basic medical research, drug development, and personalized medicine. Here, we made a deep investigation for global patents of organoid technologies in the past decade using bibliometric analysis for the first time. We have identified a total of 672 patents related to organoid technology. The number of annual patent applications exhibits an overall upward growth trend over the past decade, especially entering an exponential growth since 2015. Notably, 76.64% of patents are related to the construction of organoid models. Liver, brain, and intestinal models take up the first three places in the physiological models, while tumor models account for 76.30% of the total patents for disease models. Furthermore, drug screening is the most preferred application, revealing the great commercial value of organoid technologies in precision medicine and preclinical drug screening.
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http://dx.doi.org/10.1016/j.isci.2022.104728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307668PMC
August 2022

Metabolic engineering of Corynebacterium glutamicum for efficient production of optically pure (2R,3R)-2,3-butanediol.

Microb Cell Fact 2022 Jul 25;21(1):150. Epub 2022 Jul 25.

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering of Ministry of Education, SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

Background: 2,3-butanediol is an important platform compound which has a wide range of applications, involving in medicine, chemical industry, food and other fields. Especially the optically pure (2R,3R)-2,3-butanediol can be employed as an antifreeze agent and as the precursor for producing chiral compounds. However, some (2R,3R)-2,3-butanediol overproducing strains are pathogenic such as Enterobacter cloacae and Klebsiella oxytoca.

Results: In this study, a (3R)-acetoin overproducing C. glutamicum strain, CGS9, was engineered to produce optically pure (2R,3R)-2,3-butanediol efficiently. Firstly, the gene bdhA from B. subtilis 168 was integrated into strain CGS9 and its expression level was further enhanced by using a strong promoter P and ribosome binding site (RBS) with high translation initiation rate, and the (2R,3R)-2,3-butanediol titer of the resulting strain was increased by 33.9%. Then the transhydrogenase gene udhA from E. coli was expressed to provide more NADH for 2,3-butanediol synthesis, which reduced the accumulation of the main byproduct acetoin by 57.2%. Next, a mutant atpG was integrated into strain CGK3, which increased the glucose consumption rate by 10.5% and the 2,3-butanediol productivity by 10.9% in shake-flask fermentation. Through fermentation engineering, the most promising strain CGK4 produced a titer of 144.9 g/L (2R,3R)-2,3-butanediol with a yield of 0.429 g/g glucose and a productivity of 1.10 g/L/h in fed-batch fermentation. The optical purity of the resulting (2R,3R)-2,3-butanediol surpassed 98%.

Conclusions: To the best of our knowledge, this is the highest titer of optically pure (2R,3R)-2,3-butanediol achieved by GRAS strains, and the result has demonstrated that C. glutamicum is a competitive candidate for (2R,3R)-2,3-butanediol production.
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http://dx.doi.org/10.1186/s12934-022-01875-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310479PMC
July 2022

Effectiveness and Safety of Four Aerobic Exercise Intensity Prescription Techniques in Rehabilitation Training for Patients with Coronary Heart Disease.

Cardiol Res Pract 2022 15;2022:1647809. Epub 2022 Jul 15.

Department of Chronic Disease Management, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong, China.

Methods: A retrospective study was conducted on all patients with CHD who were admitted to CR and completed cardiopulmonary exercise tests (CPET) in Guangdong Hospital of traditional Chinese medicine. According to the risk stratification method of CHD, all participants were divided into three groups: low, moderate, and high risk. The training target heart rates (HRt) of each participant were calculated according to the formula of heart-rate-reserve (HRR), maximum-heart-rate (MHR), target-heart-rate (THR), and anaerobic threshold (AT) method provided in the guideline. Among them, the HRR method using the maximum-heart-rate obtained by the age formula was named "HRR method A," and that using the actual measured peak heart rate was named "HRR method B." For the three groups, the effectiveness and safety indexes at the target-heart-rate zone set by the different formulas above are counted and compared using CPET data.

Results: A total of 324 patients were included in the analysis. There was no significant difference between the target-heart-rate set by the HRR method A and AT method among the three groups ( > 0.05). The mean value of HRt set by other methods was lower than the AT heart rate ( < 0.05). The HRt set by the THR method was close to the AT, while that set by the MHR method was the lowest. The frequency of patients whose HRt was set by the MHR method was lower than the AT one, which was the highest. None of the participants had serious adverse events. There were no risks of ECG abnormalities in the low- and moderate-risk groups. The HRR method A had the highest incidence of various risks of ECG abnormalities, while the MHR method had the lowest one, and the safety of the THR method is close to that of the AT method ( < 0.05).

Conclusion: The heart rate calculated by HRR method A is more consistent with the actual AT. All four techniques are safe in low- and moderate-risk patients. In high-risk patients, using HRR method A has certain risks. It is recommended to use the MHR method for safety reasons, but its effectiveness is low. If considering both effectiveness and safety, the THR method can be conservatively selected at the beginning of the CR program.
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http://dx.doi.org/10.1155/2022/1647809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307357PMC
July 2022

Dermoscopy, reflectance confocal microscopy, and high-frequency ultrasound for the noninvasive diagnosis of morphea-form basal cell carcinoma.

Skin Res Technol 2022 Jul 24. Epub 2022 Jul 24.

Institute of Dermatology, Chengdu Second People's Hospital, Chengdu, Sichuan, China.

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http://dx.doi.org/10.1111/srt.13197DOI Listing
July 2022

Association Between Proton Pump Inhibitor Therapy and Spontaneous Bacterial Peritonitis Occurrence in Cirrhotic Patients: A Clinical Review.

Curr Med Sci 2022 Jul 23. Epub 2022 Jul 23.

Department and Institute of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430030, China.

Spontaneous bacterial peritonitis (SBP) is one of the most common complications in patients with end-stage liver disease (ESLD), which increases the risk of short-term mortality. Proton pump inhibitors (PPIs) are frequently used in patients with ESLD, in which controversies about the risk of PPI treatment in the occurrence of SBP are largely raised and the pathogenic mechanism of PPI-associated SBP remains unclear. We conducted a systematic literature search through PubMed/MEDLINE for publications mainly from 1 January 2000 to 1 January 2021. Our narrative review summarized the adverse effect of specific PPI therapy on the occurrence and prognosis of SBP in cirrhotic patients, described the potential mechanisms by which PPI induces the development of SBP, and discussed the risk factors associated with the development of SBP and the strategy of PPI therapy in cirrhotic patients. Although controversy regarding the association between PPI use and the occurrence of SBP exists, PPIs use should be restricted to patients with clear benefit indications, and be cautious for elderly patients with severe liver damage.
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http://dx.doi.org/10.1007/s11596-022-2607-3DOI Listing
July 2022

Stable Yellow Light-Emitting Diodes Based on Quasi-Two-Dimensional Perovskites.

ACS Appl Mater Interfaces 2022 Aug 22;14(30):34918-34925. Epub 2022 Jul 22.

Department of Physics, CAS Key Laboratory of Strongly-Coupled Quantum Matter Physics, University of Science and Technology of China, Hefei, Anhui 230026, China.

Perovskite light-emitting diodes (PeLEDs) show great potential in display and lighting because of their tunable wavelength, narrow emission bandwidths, and high color purity. Currently, the external quantum efficiency (EQE) of red and green PeLEDs has reached >23%. However, yellow PeLEDs are still rarely reported because of phase separation in mixed-halide perovskites and the coexistence of multiple phases in quasi-two-dimensional (quasi-2D) perovskites LABX ( = 1, 2, 3, ...), where L is a bulky organoammonium ligand. Here, we fabricate stable yellow PeLEDs by manipulating the phase distribution and incorporating rubidium cations (Rb) in quasi-2D perovskites. The transient absorption results confirm that alkylammonium ligand butyl ammonium (BA) has a narrower phase distribution than phenylethyl ammonium (PEA) in the quasi-2D perovskites, resulting in a more blue-shifted emission peak. We further incorporate a proper molar ratio of Rb in the (BA)CsPbI perovskite to blue-shift the emission peak to the yellow range. Finally, the yellow PeLEDs exhibit an EQE of 3.5%, and the stable emission peak is located at 595 nm. Our work provides a useful approach for the fabrication of highly efficient and stable yellow PeLEDs.
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http://dx.doi.org/10.1021/acsami.2c07314DOI Listing
August 2022
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