Publications by authors named "Tao Chen"

2,729 Publications

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Application of terahertz spectroscopy combined with density functional theory to analysis of intermolecular weak interactions for coumarin and 6-methylcoumarin.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 21;263:120159. Epub 2021 Jul 21.

Guangxi Key Laboratory of Automatic Detecting Technology and Instruments, School of Electronic Engineering and Automation, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China. Electronic address:

The terahertz (THz) absorption spectra of coumarin and 6-methylcoumarin have been investigated by terahertz time-domain spectroscopy (THz-TDS) system in the frequency range from 0.4 to 2.8 THz. Density functional theory (DFT) calculations, both with and without London force dispersion corrections, have been used for the assignment of the experimental THz spectra. To thoroughly interpret the spectrum information, we used potential energy distribution (PED) method to assign the vibrational modes of the absorption peaks, and identify the origin of the absorption peaks by electrostatic potential (ESP) and van der Waals (vdW) potential distribution analysis method. The results show that absorption peaks both for coumarin and 6-methylcoumarin are caused by electrostatic interaction in the lower frequency range, while vdW interaction in the higher frequency. Moreover, the potential energy distribution of electrostatic and vdW between them is basically the same, and it led to the similarity of THz spectra between coumarin and 6-MC. This work has demonstrated that using THz spectroscopy combined with DFT calculations is an effective way to analysis of intermolecular weak interactions and biomolecules with similar structures.
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http://dx.doi.org/10.1016/j.saa.2021.120159DOI Listing
July 2021

Controllable epitaxial growth of GeSe2 nanostructures and nonlinear optical properties.

Nanotechnology 2021 Jul 27. Epub 2021 Jul 27.

department of physics, Hunan Key Laboratory for Micro-Nano Energy Materials and Devices, Xiangtan University, Xiangtan, Hunan, 411105, CHINA.

Germanium diselenide (GeSe2) has emerged as a new member of anisotropic 2D materials and gained increasing attention because of its excellent air stability, wide bandgap and unique anisotropic properties, which exhibits promising applications in the fields of electronics, optoelectronics and polarized photodetection. However, the controllable epitaxial growth of large-scale and high-quality GeSe2 nanostructures to date remains a big challenge. Herein, GeSe2 nanofilms with lateral size up to centimeter scale have been successfully prepared on mica and 2D layered substrates forming vertical heterostructures by employing chemical vapor deposition technique. Experimental results demonstrated that hydrogen is the key factor for the controllable growth of GeSe2 nanostructures and GeSe2-based heterostructures. Corresponding growth mechanism was proposed based on systematical characterizations. The nonlinear optical properties of as-prepared GeSe2 were investigated by employing open-aperture z-scan technique exhibiting significant saturable and reverse saturable absorption behaviors at wavelengths of 400 nm and 800 nm, respectively. This study provides a new and robust route for fabricating GeSe2 nanostructures and 2D heterostructures, which will benefit the development of GeSe2-based nonlinear optical and optoelectronic devices.
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http://dx.doi.org/10.1088/1361-6528/ac1801DOI Listing
July 2021

Imbalance Model of Heart Rate Variability and Pulse Wave Velocity in Psychotic and Nonpsychotic Disorders.

Schizophr Bull 2021 Jul 27. Epub 2021 Jul 27.

Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiaotong University School of Medicine, Shanghai, PR China.

Objectives: Patients with psychiatric disorders have an increased risk of cardiovascular pathologies. A bidirectional feedback model between the brain and heart exists widely in both psychotic and nonpsychotic disorders. The aim of this study was to compare heart rate variability (HRV) and pulse wave velocity (PWV) functions between patients with psychotic and nonpsychotic disorders and to investigate whether subgroups defined by HRV and PWV features improve the transdiagnostic psychopathology of psychiatric classification.

Methods: In total, 3448 consecutive patients who visited psychiatric or psychological health services with psychotic (N = 1839) and nonpsychotic disorders (N = 1609) and were drug-free for at least 2 weeks were selected. HRV and PWV indicators were measured via finger photoplethysmography during a 5-minute period of rest. Canonical variates were generated through HRV and PWV indicators by canonical correlation analysis (CCA).

Results: All HRV indicators but none of the PWV indicators were significantly reduced in the psychotic group relative to those in the nonpsychotic group. After adjusting for age, gender, and body mass index, many indices of HRV were significantly reduced in the psychotic group compared with those in the nonpsychotic group. CCA analysis revealed 2 subgroups defined by distinct and relatively homogeneous patterns along HRV and PWV dimensions and comprising 19.0% (subgroup 1, n = 655) and 80.9% (subgroup 2, n = 2781) of the sample, each with distinctive features of HRV and PWV functions.

Conclusions: HRV functions are significantly impaired among psychiatric patients, especially in those with psychosis. Our results highlight important subgroups of psychiatric patients that have distinct features of HRV and PWV which transcend current diagnostic boundaries.
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http://dx.doi.org/10.1093/schbul/sbab080DOI Listing
July 2021

Aggregation Induced Emissive Carbon Dots Gels for Octopus-Inspired Shape/Color Synergistically Adjustable Actuator.

Angew Chem Int Ed Engl 2021 Jul 26. Epub 2021 Jul 26.

Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences: Ningbo Institute of Industrial Technology Chinese Academy of Sciences, Department of polymer, 315201, Ningbo, CHINA.

Some living organisms such as the octopus have fantastic abilities to simultaneously swim away and alter body color/morphology for disguise and self-protection, especially when there is a threat perception. However, it is still quite challenging to construct artificial soft actuators with octopus-like synergistic shape/color change and directional locomotion behaviors, but such systems could enhance the functions of soft robotics dramatically. Herein, we proposed to utilize unique hydrophobic carbon dots (CDs) with rotatable surficial groups to construct the aggregation-induced emission (AIE) active glycol CDs polymer gel, which could be further employed to be interfacially bonded to an elastomer to produce anisotropic bilayer soft actuator. When putting the actuator on a water surface, glycol spontaneously diffused out from the gel layer to allow water intake, resulting in a color change from a blue dispersion fluorescence to red AIE and a shape deformation, as well as a large surface tension gradient that can promote its autonomous locomotion. Based on these findings, artificial soft swimming robots with octopus-like synergistic shape/color change and directional swimming motion were demonstrated. This study provides an elegant strategy to develop advanced multi-functional bio-inspired intelligent soft robotics.
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http://dx.doi.org/10.1002/anie.202107281DOI Listing
July 2021

sQuiz your knowledge! A case of dermatitis, psychosis, and diarrhoea.

Eur J Dermatol 2021 Jun;31(3):435-436

Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu, Sichuan, China.

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http://dx.doi.org/10.1684/ejd.2021.4076DOI Listing
June 2021

Proton Pump Inhibitor Therapy Increases the Risk of Spontaneous Bacterial Peritonitis in Patients with HBV-Related Acute-on-Chronic Liver Failure.

Adv Ther 2021 Jul 25. Epub 2021 Jul 25.

Department and Institute of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095, Jiefang Avenue, Wuhan, 430030, People's Republic of China.

Introduction: Spontaneous bacterial peritonitis (SBP) is a common infection in patients with hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF). SBP significantly increases the mortality rate and medical costs. The association between proton pump inhibitor (PPI) use and SBP remains unclear. We conducted a retrospective study to investigate the association between PPI use and SBP in patients with HBV-related ACLF and to explore the risk factors for SBP.

Methods: We compared the SBP incidence between the PPI and non-PPI groups before and after propensity score matching and explored the association between the duration and type of PPI and SBP occurrence. Risk factors for SBP occurrence were determined by univariate and multivariate logistic regression analysis.

Results: The SBP incidence was higher in the PPI group than in the non-PPI group before and after propensity score matching. The SBP incidence increased for elevated MELD scores in PPI users. There was a similar SBP incidence in both different types and durations of PPI users. MELD score, old age, male sex, and high WBC count were significant independent risk factors for SBP in PPI users with HBV-related ACLF in the hospital.

Conclusions: PPI therapy increases the risk of SBP development in patients with HBV-related ACLF. MELD score, old age, male sex, and high WBC count could serve as predictors of SBP in PPI users. Caution should be taken regarding PPI use, especially for patients with MELD scores > 30.
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http://dx.doi.org/10.1007/s12325-021-01844-1DOI Listing
July 2021

Quantification of the uncertainties within the radiotherapy dosimetry chain and their impact on tumour control.

Phys Imaging Radiat Oncol 2021 Jul 28;19:33-38. Epub 2021 Jun 28.

Department of Chemical and Process Engineering, University of Surrey, Guildford, UK.

Background And Purpose: Dose delivered during radiotherapy has uncertainty arising from a number of sources including machine calibration, treatment planning and delivery and can impact outcomes. Any systematic uncertainties will impact all patients and can continue for extended periods. The impact on tumour control probability (TCP) of the uncertainties within the radiotherapy calibration process has been assessed.

Materials And Methods: The linear-quadratic model was used to simulate the TCP from two prostate cancer and a head and neck (H&N) clinical trial. The uncertainty was separated into four components; 1) initial calibration, 2) systematic shift due to output drift, 3) drift during treatment and 4) daily fluctuations. Simulations were performed for each clinical case to model the variation in TCP present at the end of treatment arising from the different components.

Results: Overall uncertainty in delivered dose was +/-2.1% (95% confidence interval (CI)), consisting of uncertainty standard deviations of 0.7% in initial calibration, 0.8% due to subsequent calibration shift due to output drift, 0.1% due to drift during treatment, and 0.2% from daily variations. The overall uncertainty of TCP (95% CI) for a population of patients treated on different machines was +/-3%, +/-5%, and +/-3% for simulations based on the two prostate trials and H&N trial respectively.

Conclusion: The greatest variation in delivered target volume dose arose from calibration shift due to output drift. Careful monitoring of beam output following initial calibration remains vital and may have a significant impact on clinical outcomes.
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http://dx.doi.org/10.1016/j.phro.2021.06.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295844PMC
July 2021

When and Why Contexts Predict Unethical Behavior: Evidence From a Laboratory Bribery Game.

Front Psychol 2021 9;12:675319. Epub 2021 Jul 9.

Big Data Research Lab, Department of Economics, University of Waterloo, Waterloo, ON, Canada.

In economic unethical decision-making experiments, one important methodological investigation is what types of contexts should be used to frame the instructions. Within the experimental economics community, using neutral-context instructions instead of loaded-context instructions is the mainstream practice. Because the loaded contexts may impact behavior in an unpredictable manner and therefore, put experimental control at risk. Nevertheless, using the loaded-context instructions could be advantageous in several ways. A properly framed context can help to facilitate learning and gain ecological validity. The challenge is whether we can identify when and why the loaded context may alter behavior. In this paper, we aim to test if being familiar with a loaded context can systematically influence unethical decisions in a bribery game. We conduct a laboratory bribery game experiment with three different treatments: the neutral-context treatment, the familiar-context treatment, and the unfamiliar-context treatment. Using the neutral-context treatment as a benchmark, we find that participants in the familiar-context treatment express stronger negative attitudes toward corruption. Attitudes toward unethical behavior are the same in the neutral-context treatment and the unfamiliar-context treatment. Behaviorally, the participants in the familiar-context treatment are much less likely to engage in corrupt activities. The neutral-context treatment and the unfamiliar-context treatment produce the same behavioral outcome.
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http://dx.doi.org/10.3389/fpsyg.2021.675319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299706PMC
July 2021

Dynamic nomogram for persistent organ failure in acute biliary pancreatitis: Development and validation in a retrospective study.

Dig Liver Dis 2021 Jul 22. Epub 2021 Jul 22.

Department of Gastroenterology, Affiliated Hospital of Southwest Medical University, Luzhou, China. Electronic address:

Background: Persistent organ failure (POF) increases the risk of death in patients with acute biliary pancreatitis (ABP). Currently, there is no early risk assessment tool for POF in patients with ABP.

Aims: To establish and validate a dynamic nomogram for predicting the risk of POF in ABP.

Methods: This was a retrospective study of 792 patients with ABP, with 595 cases in the development group and 197 cases in the validation group. Least absolute shrinkage and selection operator regression screened the predictors of POF, and logistic regression established the model (P < 0.05). A dynamic nomogram showed the model. We evaluated the model's discrimination, calibration, and clinical effectiveness; used the bootstrap method for internal validation; and conducted external validation in the validation group.

Results: Neutrophils, haematocrit, serum calcium, and blood urea nitrogen were predictors of POF in ABP. In the development group and validation group, the areas under the receiver operating characteristic curves (AUROCs) were 0.875 and 0.854, respectively, and the Hosmer-Lemeshow test (P > 0.05) and calibration curve showed good consistency between the actual and prediction probability. Decision curve analysis showed that the dynamic nomogram has excellent clinical value.

Conclusion: This dynamic nomogram helps with the early identification and screening of high-risk patients with POF in ABP.
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http://dx.doi.org/10.1016/j.dld.2021.06.033DOI Listing
July 2021

Acinetobacter baumannii Strains Isolated from CSF and Bloodstream Analysed by cgMLST: The Dominance of the CC92 Clonal Complex in CSF Infections.

Int J Antimicrob Agents 2021 Jul 20:106404. Epub 2021 Jul 20.

Department of Infectious Diseases, Sir Run Run Shaw Hospital, School of Medicine Zhejiang University, Hangzhou, China; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China.. Electronic address:

Fifty-four unique clinical A. baumannii strains isolated from cerebrospinal fluid (CSF) together with 21 bloodstream isolates collected from 5 tertiary hospitals in East China between April 2013 and November 2016 were studied for susceptibility patterns, and the prevalence of resistance genes. The molecular epidemiological characteristics of CSF isolates and the phylogenetic relationships of isolates from different sources were assessed using multi-locus sequence typing (MLST) and core genome multi-locus sequence typing (cgMLST). A total of 94.4% (51/54) of CSF isolates were blacarrying carbapenem-resistant A. baumannii (CRAB). Their average resistance rate to different classes of antibiotics was extremely high (>90%), except for tigecycline and colistin. According to the Oxford MLST scheme, all of CSF isolates fell into 10 defined STs and 4 novel STs. ST195 and ST208 were the leading STs in isolates from either source. Fifty CSF isolates and 20 bloodstream isolates were assigned to clonal complex 92 (CC92), revealing a wild distribution of CC92 in the hospitals of East China. In combination with epidemiological data linked in time and space, the cgMLST results elucidated intra- and inter-hospital polyclonal dissemination of A. baumannii meningitis. Based on cgMLST, there was no correlation between phylogeny and the source of isolation in A. baumannii. These results emphasize that the genetic potential of this pathogen is vast enough to infect multiple human body sites.
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http://dx.doi.org/10.1016/j.ijantimicag.2021.106404DOI Listing
July 2021

A Novel Post-Operative ALRI Model Accurately Predicts Clinical Outcomes of Resected Hepatocellular Carcinoma Patients.

Front Oncol 2021 6;11:665497. Epub 2021 Jul 6.

Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Hepatocellular carcinoma (HCC) is one of the leading malignant tumors worldwide. Prognosis and long-term survival of HCC remain unsatisfactory, even after radical resection, and many non-invasive predictors have been explored for post-operative patients. Most prognostic prediction models were based on preoperative clinical characteristics and pathological findings. This study aimed to investigate the prognostic value of a newly constructed nomogram, which incorporated post-operative aspartate aminotransferase to lymphocyte ratio index (ALRI).

Methods: A total of 771 HCC patients underwent radical resection from three medical centers were enrolled and grouped into the training cohort (n = 416) and validation cohort (n = 355). Prognostic prediction potential of ALRI was assessed by receiver operating curve (ROC) analysis. The Cox regression model was used to identify independent prognostic factors. Nomograms for overall survival (OS) and disease-free survival (DFS) were constructed and further validated externally.

Results: The ROC analysis ranked ALRI as the most effective prediction marker for resected HCC patients, with the cut-off value determined at 22.6. Higher ALRI level positively correlated with larger tumor size, higher tumor node metastasis (TNM) stage, and inversely with lower albumin level and shorter OS and DFS. Nomograms for OS and DFS were capable of discriminating HCC patients into different risk-groups.

Conclusions: Post-operative ALRI was of prediction value for HCC prognosis. This novel nomogram may categorize HCC patients into different risk groups, and offer individualized surveillance reference for post-operative patients.
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http://dx.doi.org/10.3389/fonc.2021.665497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290124PMC
July 2021

Atomically Precise Dinuclear Site Active toward Electrocatalytic CO Reduction.

J Am Chem Soc 2021 Jul 22. Epub 2021 Jul 22.

National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230029, PR China.

The development of atomically precise dinuclear heterogeneous catalysts is promising to achieve efficient catalytic performance and is also helpful to the atomic-level understanding on the synergy mechanism under reaction conditions. Here, we report a Ni(dppm)Cl dinuclear-cluster-derived strategy to a uniform atomically precise Ni site, consisting of two Ni-N moieties shared with two nitrogen atoms, anchored on a N-doped carbon. By using synchrotron X-ray absorption spectroscopy, we identify the dynamically catalytic dinuclear Ni structure under electrochemical CO reduction reaction, revealing an oxygen-bridge adsorption on the Ni-N site to form an O-Ni-N structure with enhanced Ni-Ni interaction. Theoretical simulations demonstrate that the key O-Ni-N structure can significantly lower the energy barrier for CO activation. As a result, the dinuclear Ni catalyst exhibits >94% Faradaic efficiency for efficient carbon monoxide production. This work provides bottom-up target synthesis approaches and evidences the identity of dinuclear sites active toward catalytic reactions.
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http://dx.doi.org/10.1021/jacs.1c05754DOI Listing
July 2021

The R168G heterozygous mutation of tropomyosin 3 (TPM3) was identified in three family members and has manifestations ranging from asymptotic to serve scoliosis and respiratory complications.

Genes Dis 2021 Sep 25;8(5):715-720. Epub 2020 Jan 25.

Department of Orthopedic, Chongqing Children's Hospital, Chongqing Medical University, No. 136 of Zhong Shan Er Lu, Chongqing, 400014, PR China.

According to existing reports, mutations in the slow tropomyosin gene () may lead to congenital fiber-type disproportion (CFTD), nemaline myopathy (NM) and cap myopathy (CD). They are all congenital myopathies and are associated with clinical, pathological and genetic heterogeneity. A ten-year-old girl with scoliosis was unable to wean from mechanical ventilation after total intravenous anesthesia. The girl has scoliosis, respiratory insufficiency, motion delay and muscle weakness; her younger brother has a similar physiology but does not have scoliosis or respiratory insufficiency, and her parents are healthy. We conducted genetic testing and found a c.502C > G (p.R168G) heterozygous mutation in the family. This mutation originated from the father and was autosomal dominant. Muscle biopsy results indicated that no special structures were present, and the type I fiber ratio was not notably high compared to previous reports. Although the family members have the same mutations, their clinical manifestations are quite different.
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http://dx.doi.org/10.1016/j.gendis.2020.01.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278530PMC
September 2021

Ridge preservation applying a novel hydrogel for early angiogenesis and osteogenesis evaluation: an experimental study in canine.

J Biol Eng 2021 Jul 21;15(1):19. Epub 2021 Jul 21.

Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing, 401147, P. R. China.

Ridge preservation is universally acknowledged as the conventional method for the post-extraction healing yet there are no standard materials for the ideal healing outcome. Herein, a composite gel comprising gelatin nanoparticles (GNPs) and injectable platelet-rich-fibrin (i-PRF) as the potential candidate for extracted socket healing is introduced. The combination of GNPs and i-PRF not only possesses favorable mechanical properties to withstand external force but also accelerate the blood clotting time significantly. In addition, six beagle dogs were adopted to assess the angiogenic and osteogenic capacity of GNPs+i-PRF gel in vivo. The GNPs+i-PRF gel significantly produced the most blood vessels area, woven bone and low osteoclast activity in extracted sockets at 2 weeks postoperation and remarkably generated corticalization on the alveolar ridge crest at 8 weeks postoperation according to histological results. Therefore, GNPs+i-PRF gel can be recommended as the candidate grafting material regarding ridge preservation for its cost effectiveness, excellent biocompatibility, facilitation of blood clotting and favorable capacity of promoting angiogenesis and osteogenesis.
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http://dx.doi.org/10.1186/s13036-021-00271-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293569PMC
July 2021

Oxytocin in the anterior cingulate cortex attenuates neuropathic pain and emotional anxiety by inhibiting presynaptic long-term potentiation.

Cell Rep 2021 Jul;36(3):109411

Center for Neuron and Disease, Frontier Institutes of Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China; Department of Physiology, Faculty of Medicine, University of Toronto, Medical Science Building, 1 King's College Circle, Toronto, ON M5S 1A8, Canada; Institute of Brain Research, Qingdao International Academician Park, Qingdao, Shandong, China. Electronic address:

Oxytocin is a well-known neurohypophysial hormone that plays an important role in behavioral anxiety and nociception. Two major forms of long-term potentiation, presynaptic LTP (pre-LTP) and postsynaptic LTP (post-LTP), have been characterized in the anterior cingulate cortex (ACC). Both pre-LTP and post-LTP contribute to chronic-pain-related anxiety and behavioral sensitization. The roles of oxytocin in the ACC have not been studied. Here, we find that microinjections of oxytocin into the ACC attenuate nociceptive responses and anxiety-like behavioral responses in animals with neuropathic pain. Application of oxytocin selectively blocks the maintenance of pre-LTP but not post-LTP. In addition, oxytocin enhances inhibitory transmission and excites ACC interneurons. Similar results are obtained by using selective optical stimulation of oxytocin-containing projecting terminals in the ACC in animals with neuropathic pain. Our results demonstrate that oxytocin acts on central synapses and reduces chronic-pain-induced anxiety by reducing pre-LTP.
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http://dx.doi.org/10.1016/j.celrep.2021.109411DOI Listing
July 2021

Functional Evaluation of the Signal Peptides of Secreted Proteins.

Bio Protoc 2018 May 5;8(9):e2839. Epub 2018 May 5.

College of Plant Science and Technology, Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, and Key Lab of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, China.

Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily.
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http://dx.doi.org/10.21769/BioProtoc.2839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275294PMC
May 2018

Periodic root branching is influenced by light through an HY1-HY5-auxin pathway.

Curr Biol 2021 Jul 9. Epub 2021 Jul 9.

MOA Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River and State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

The spacing of lateral roots (LRs) along the main root in plants is driven by an oscillatory signal, often referred to as the "root clock" that represents a pre-patterning mechanism that can be influenced by environmental signals. Light is an important environmental factor that has been previously reported to be capable of modulating the root clock, although the effect of light signaling on the LR pre-patterning has not yet been fully investigated. In this study, we reveal that light can activate the transcription of a photomorphogenic gene HY1 to maintain high frequency and amplitude of the oscillation signal, leading to the repetitive formation of pre-branch sites. By grafting and tissue-specific complementation experiments, we demonstrated that HY1 generated in the shoot or locally in xylem pole pericycle cells was sufficient to regulate LR branching. We further found that HY1 can induce the expression of HY5 and its homolog HYH, and act as a signalosome to modulate the intracellular localization and expression of auxin transporters, in turn promoting auxin accumulation in the oscillation zone to stimulate LR branching. These fundamental mechanistic insights improve our understanding of the molecular basis of light-controlled LR formation and provide a genetic interconnection between shoot- and root-derived signals in regulating periodic LR branching.
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http://dx.doi.org/10.1016/j.cub.2021.06.055DOI Listing
July 2021

Serum neurofilament light chain or glial fibrillary acidic protein in the diagnosis and prognosis of brain metastases.

J Neurol 2021 Jul 20. Epub 2021 Jul 20.

State Key Laboratory of Respiratory Disease, National Clinical Research Centre for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital, Guangzhou Medical University, 151# Yanjiang Road, Guangzhou, 510120, China.

Introduction: Brain metastases (BM) remains the most cumbersome disease burden in patients with lung cancer. This study aimed to investigate whether serum brain injury biomarkers can indicate BM, to further establish related diagnostic models, or to predict prognosis of BM.

Materials And Methods: This was a prospective study of patients diagnosed with lung cancer with BM (BM group), with lung cancer without BM (NBM group), and healthy participants (control group). Serum neurofilament light chain (NfL) and glial fibrillary acidic protein (GFAP) were detected at baseline. We identified and integrated the risk factors of BM to establish diagnostic models.

Results: A total of 158 patients were included (n = 37, 57, and 64 in the BM, NBM, and control groups, respectively). Serum biomarker levels were significantly higher in the NBM group than in the control group. Higher serum NfL and GFAP concentrations were associated with BM (odds ratios, 3.06 and 1.79, respectively). NfL (area under curve [AUC] = 0.77, p < 0.001) and GFAP (AUC = 0.64, p = 0.02) had diagnostic value for BM. The final diagnostic model included NfL level, age, Karnofsky Performance Status. The model had an AUC value of 0.83 (95% confidence interval [CI] 0.75-0.92). High NfL concentration was correlated with poor overall survival of patients with BM (hazard ratio, 3.31; 95% CI 1.22-9.04; p = 0.019).

Conclusion: Serum NfL and GFAP could be potential diagnostic biomarkers for BM in patients with lung cancer. We established a model that can provide individual diagnoses of BM. Higher NfL level may be associated with poor prognosis of patients with BM.
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http://dx.doi.org/10.1007/s00415-021-10660-0DOI Listing
July 2021

Retinal neovascularization induced by mutant gene inhibited in an inherited retinitis pigmentosa mouse model: an study.

Int J Ophthalmol 2021 18;14(7):990-997. Epub 2021 Jul 18.

Center of Clinical Aerospace Medicine, Air Force Medical University, Xi'an 710032, Shaanxi Province, China.

Aim: To explore whether the retinal neovascularization (NV) in a genetic mutant mice model could be ameliorated in an inherited retinitis pigmentosa (RP) mouse, which would help to elucidate the possible mechanism and prevention of retinal NV diseases in clinic.

Methods: The mice, the genetic mutant mouse model of retinal NV caused by the homozygous mutation of gene, with the mice, the inherited RP mouse caused by homozygous mutation of gene were bred. Intercrossing of the above two mice led to the birth of the F1 hybrids, further inbreeding of which gave birth to the F2 offspring. The ocular genotypes and phenotypes of the mice from all generations were examined, with the F2 offspring grouped according to the genotypes.

Results: The mice exhibited the RP phenotype of outer retinal degeneration and loss of retinal function. The mice exhibited the phenotype of retinal NV obviously shown by the fundus fluorescein angiography. The F1 hydrides, with the heterozygote genotype, exhibited no phenotypes of RP or retinal NV. The F2 offspring with homozygous genotypes were grouped into four subgroups. They were the F2-I mice with the wild-type and genes ( - ), which had normal ocular phenotypes; the F2-II mice with homozygous mutant gene ( - ), which exhibited the retinal NV phenotype; the F2-III mice with homozygous mutant gene ( - ), which exhibited the RP phenotype. Specifically, the F2-IV mice with homozygous mutant and gene ( - ) showed only the RP phenotype, without the signs of retinal NV.

Conclusion: The retinal NV can be inhibited by the RP phenotype, which implies the role of a hyperoxic state in treating retinal NV diseases.
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http://dx.doi.org/10.18240/ijo.2021.07.05DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243196PMC
July 2021

Improvement of a SARS-CoV-2 vaccine by enhancing the conjugation efficiency of the immunogen to self-assembled nanoparticles.

Cell Mol Immunol 2021 Jul 19. Epub 2021 Jul 19.

Institute of Human Virology, Key Laboratory of Tropical Disease Control of the Ministry of Education, Guangdong Engineering Research Center for Antimicrobial Agents and Immunotechnology, Engineering Research Center of Gene Vaccines of the Ministry of Education, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, Guangdong, China.

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http://dx.doi.org/10.1038/s41423-021-00736-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287548PMC
July 2021

Association between the HGF/c‑MET signaling pathway and tumorigenesis, progression and prognosis of hepatocellular carcinoma (Review).

Authors:
Wei Meng Tao Chen

Oncol Rep 2021 Sep 19;46(3). Epub 2021 Jul 19.

School of Medicine, China Three Gorges University, Yichang, Hubei 443002, P.R. China.

Hepatocellular carcinoma (HCC) is one of the most aggressive and lethal malignancies with a rising incidence, and is characterized by rapid progression, frequent metastasis, late diagnosis, high postoperative recurrence and poor prognosis. Therefore, novel treatment strategies for HCC, particularly advanced HCC, are urgently required. The hepatocyte growth factor (HGF)/c‑mesenchymal‑epithelial transition receptor (c‑MET) axis is a key signaling pathway in HCC and is strongly associated with its highly malignant features. Available treatments based on HGF/c‑MET inhibition may prolong the lifespan of patients with HCC; however, they do not achieve the desired therapeutic effects. The aim of the present article was to review the basic knowledge regarding the role of the HGF/c‑MET signaling pathway in HCC, and examine the association between the HGF/c‑MET signaling pathway and the tumorigenesis, progression and prognosis of HCC.
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http://dx.doi.org/10.3892/or.2021.8142DOI Listing
September 2021

Neurocognitive assessments are more important among adolescents than adults for predicting psychosis in clinical high risk.

Biol Psychiatry Cogn Neurosci Neuroimaging 2021 Jul 15. Epub 2021 Jul 15.

Shanghai Mental Health Center, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Psychotic Disorders, Shanghai 200030, PR China; CAS Center for Excellence in Brain Science and Intelligence Technology (CEBSIT), Chinese Academy of Science, PR China; Brain Science and Technology Research Center, Shanghai Jiao Tong University, Shanghai, China.

Background: Few studies have examined the effects of age on neurocognition to predict conversion to psychosis in individuals with clinical high-risk(CHRs). This study aimed to compare the extent and predictive performance of cognitive deficits between adolescents and adults with CHR.

Methods: A comprehensive neuropsychological battery was performed on 325 CHRs and 365 healthy controls(HCs). The subjects were first divided into 189 CHR adolescents(age 12-17 years), 136 CHR adults(age 18-45 years), 88 HC adolescents, and 277 HC adults. CHR subjects were then divided into converters(CHR-Cs: adolescents[n=43]; adults[n=34]) and non-converters(CHR-NCs: adolescents [n=146], adults [n=102]) based on their 2-year follow-up clinical status.

Results: The adolescent and adult CHRs performed significantly worse than their control groups on all the neurocognitive tests, except for performance on the continuous performance test in adolescents. In the comparison between adolescents and adults, patterns of neurocognitive deficits seemed to vary in HCs, rather than in CHRs. In the comparison between CHRs and HCs, the rank order of effect sizes across the neurocognitive tests was similar for the top two tests of symbol coding and verbal learning. Comparison between CHR-Cs and CHR-NCs revealed that adolescent CHR-Cs performed significantly worse than CHR-NCs on seven of eight neurocognitive tests; however, adult CHR-Cs performed significantly worse than CHR-NCs only in the visuospatial memory test.

Conclusions: The role of neurocognitive dysfunction may have different patterns and weights during the onset of psychosis in adolescent and adult CHRs, implicating the development of specific strategies that could monitor and improve cognitive function in adolescents with CHR.
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http://dx.doi.org/10.1016/j.bpsc.2021.06.015DOI Listing
July 2021

Inhibition of miR-134-5p protects against kainic acid-induced excitotoxicity through Sirt3-mediated preservation of mitochondrial function.

Epilepsy Res 2021 Jul 12;176:106722. Epub 2021 Jul 12.

Department of Neurosurgery, The 904th Hospital of PLA, Medical School of Anhui Medical University, Wuxi, Jiangsu 214044, China; Department of Neurosurgery, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, China. Electronic address:

Epilepsy is a neurological disorder which is characterized by brain hyper-excitability and manifests as seizure. Due to its complicated pathogenesis, treatment for epilepsy still remains a huge challenge for neurology in the whole world. MciroRNA-134 (miR-134) is one kind of miRNAs which was firstly found abundant in synapses. In this study, we tried to unveil the role of inhibiting MciroRNA-134-5p (miR-134-5p) in excitotoxicity induced by kainic acid (KA) in the hippocampal neurons (HT22) cells. The results showed that treatment of KA increased the expression of miR-134-5p significantly and caused marked neuron excitotoxicity, evidenced by risen cell death rate, higher LDH release and aggravated cell viability. After suppressing miR-134-5p expression via transfecting HT22 cells with miR-134-5p antisense (Anti-134), cell viability was promoted obviously, along with decreased LDH release and cell death rate. In addition, KA-induced lipid peroxidation, cytochrome c release and mitochondrial ROS generation were also attenuated by Anti-134. The level of Sirtuin 3 (Sirt3) and its downstream antioxidant enzymes, such as mitochondrial superoxide dismutase 2 (SOD2), isocitrate dehydrogenase 2 (IDH2) and glutathione peroxidase (GSH-Px), were significantly higher in Anti-134 group compared with the control and scramble group. After inhibiting Sirt3 expression with SiRNA targeting Sirt3 (Si-Sirt3) and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP), the positive role of Anti-134 was apparently reversed. In conclusion, this research highly suggests that inhibition of miR-134-5p could protect neurons from KA-induced excitotoxicity through Sirt3-mediated preservation of mitochondrial function.
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http://dx.doi.org/10.1016/j.eplepsyres.2021.106722DOI Listing
July 2021

Analysis of the Resistance of the Meshes to Infection.

Front Surg 2021 24;8:644227. Epub 2021 Jun 24.

Department of Biliary-Pancreatic Surgery, School of Medicine, Renji Hospital, Shanghai Jiao Tong University, Shanghai, China.

The mesh infection is mostly related to the gram-negative bacteria, such as Escherichia coli () for emergency surgery of incarcerated hernia. However, few study investigated the effects of concentration, mesh materials and antibiotic prophylaxis on mesh infection after hernioplasty. The aim of this study was to evaluate the bacterial resistance to for three different materials of mesh, and to measure the minimum concentration for mesh infection with and without antibiotic prophylaxis in a rat model. Three types of mesh (polytetrafluoroethylene, polypropylene, and biologic meshes) were used in the repair of an acute ventral hernia rat model in the setting of different concentrations of loads and antibiotics. At the 8th day after surgery, mesh samples were sent for microbiologic and histologic analyses. The positive rates of bacterial culture increased with concentration. The biologic mesh showed better bacterial resistance compared to polytetrafluoroethylene mesh and polypropylene mesh when the concentration of ranges from 10 CFU/ml to 10 CFU/ml ( = 0.002 and = 0.029, respectively). Prophylactical ceftriaxone treatment could not decrease the colonization rate of at 10 CFU/ml or 10 CFU/ml in each group ( > 0.05). The scores of neovascularization in polypropylene mesh and biologic mesh were similar, which was higher than that of polytetrafluoroethylene mesh ( < 0.05). Compared with other meshes, biologic mesh showed better tolerance to 10 CFU/ml with respect to inflammation, depth of inflammation, neovascularization, cellular repopulation and foreign body giant cells. The biologic mesh had better resistance compared to polytetrafluoroethylene mesh and polypropylene mesh when the concentration is higher than 10 CFU/ml in rats. Antibiotic prophylaxis was useful when the contamination was not particularly severe.
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http://dx.doi.org/10.3389/fsurg.2021.644227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264128PMC
June 2021

Regulating the Self-Discharge of Flexible All-Solid-State Supercapacitors by a Heterogeneous Polymer Electrolyte.

Small 2021 Jul 9:e2102054. Epub 2021 Jul 9.

Shanghai Key Lab of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, Shanghai, 200092, China.

Supercapacitors with high power density and an ultralong cyclic lifetime have been intensively investigated. However, the crucial challenge of their rapid self-discharge process is often neglected in most cases. A heterogeneous interface formed between two layers of polymer electrolytes is designed, in which a polyanion and a polycation are added into a common matrix of polymer electrolyte, respectively. By using the heterogeneous polymer electrolyte (HPE) as the separator simultaneously, the resultant supercapacitors exhibit comparable electrochemical performance to that of devices based on traditional polymer electrolytes. The HPE-based supercapacitors using both electric double-layer capacitive and pseudocapacitive electrodes show at least one time longer self-discharge time than that of devices based on homogenous polymer electrolyte, especially for the electrode in an electrolyte containing polyanion served as a positive pole during the charging process. Because of the same polymer matrix used, the heterojunction structure of the HPE exhibits excellent stability without obvious phase separation during thousands of charge/discharge and repeated bending cycles. This novel strategy by interface engineering of electrolyte to suppress the self-discharge behavior of supercapacitors is very meaningful to promote their practical applications.
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http://dx.doi.org/10.1002/smll.202102054DOI Listing
July 2021

LncRNA RCAT1 promotes tumor progression and metastasis via miR-214-5p/E2F2 axis in renal cell carcinoma.

Cell Death Dis 2021 Jul 9;12(7):689. Epub 2021 Jul 9.

Department of Urology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

Renal cell carcinoma is the second malignant tumors in the urinary system with high mortality and morbidity. Increasing evidence suggests that long non-coding RNAs (lncRNAs) play critical roles in tumor development and progression. In the current study, based on the publicly available data obtained from GEO and TCGA database, we identified five prognosis-related lncRNAs with the ability to predict the prognosis of patients with renal cell carcinoma. Among them, the uncharacterized and upregulated lncRNA RCAT1 (renal cancer-associated transcript 1) was identified as the key lncRNA. Our data further revealed that the expression of lncRNA RCAT1 was significantly upregulated in renal cell carcinoma tissues and cells. Gain-of-function and loss-of-function studies showed that lncRNA RCAT1 promoted cell proliferation, migration, and invasion in vitro and in vivo. Furthermore, we verified that lncRNA RCAT1 could abundantly sponge miR-214-5p, which served as a tumor suppressor in renal cell carcinoma. Significantly, miR-214-5p overexpression could attenuate the promotion of cell proliferation and metastasis induced by lncRNA RCAT1. Moreover, we found that E2F2 was a direct target of miR-214-5p, and lncRNA RCAT1 could protect E2F2 from miR-214-5p-mediated degradation. Taken together, our findings suggested that lncRNA RCAT1 could enhance the malignant phenotype of renal cell carcinoma cells by modulating miR-214-5p/E2F2 axis, and lncRNA RCAT1 might be a novel prognostic biomarker and a potential therapeutic target for renal cell carcinoma.
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http://dx.doi.org/10.1038/s41419-021-03955-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270952PMC
July 2021

Validation and minimum important difference of the UCSD Shortness of Breath Questionnaire in fibrotic interstitial lung disease.

Respir Res 2021 Jul 8;22(1):202. Epub 2021 Jul 8.

Department of Medicine, University of British Columbia, Vancouver, BC, Canada.

Rationale: The University of California, San Diego Shortness of Breath Questionnaire (UCSDSOBQ) is a frequently used domain-specific dyspnea questionnaire; however, there is little information available regarding its use and minimum important difference (MID) in fibrotic interstitial lung disease (ILD). We aimed to describe the key performance characteristics of the UCSDSOBQ in this population.

Methods: UCSDSOBQ scores and selected anchors were measured in 1933 patients from the prospective multi-center Canadian Registry for Pulmonary Fibrosis. Anchors included the St. George's Respiratory Questionnaire (SGRQ), European Quality of Life 5 Dimensions 5 Levels questionnaire (EQ-5D-5L) and EQ visual analogue scale (EQ-VAS), percent-predicted forced vital capacity (FVC%), diffusing capacity of the lung for carbon monoxide (DLCO%), and 6-min walk distance (6MWD). Concurrent validity, internal consistency, ceiling and floor effects, and responsiveness were assessed, followed by estimation of the MID by anchor-based (linear regression) and distribution-based methods (standard error of measurement).

Results: The UCSDSOBQ had a high level of internal consistency (Cronbach's alpha = 0.97), no obvious floor or ceiling effect, strong correlations with SGRQ, EQ-5D-5L, and EQ-VAS (|r| > 0.5), and moderate correlations with FVC%, DLCO%, and 6MWD (0.3 < |r| < 0.5). The MID estimate for UCSDSOBQ was 5 points (1-8) for the anchor-based method, and 4.5 points for the distribution-based method.

Conclusion: This study demonstrates the validity of UCSDSOBQ in a large and heterogeneous population of patients with fibrotic ILD, and provides a robust MID estimate of 5-8 points.
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http://dx.doi.org/10.1186/s12931-021-01790-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265065PMC
July 2021

Glycoursodeoxycholic acid ameliorates diet-induced metabolic disorders with inhibiting endoplasmic reticulum stress.

Clin Sci (Lond) 2021 Jul;135(14):1689-1706

Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Recent studies reveal that bile acid metabolite composition and its metabolism are changed in metabolic disorders, such as obesity, type 2 diabetes and metabolic associated fatty liver disease (MAFLD), yet its role and the mechanism remain largely unknown. In the present study, metabolomic analysis of 163 serum and stool samples of our metabolic disease cohort was performed, and we identified glycoursodeoxycholic acid (GUDCA), glycine-conjugated bile acid produced from intestinal bacteria, was decreased in both serum and stool samples from patients with hyperglycemia. RNA-sequencing and quantitative PCR results indicated that GUDCA alleviated endoplasmic reticulum (ER) stress in livers of high fat diet (HFD)-fed mice without alteration of liver metabolism. In vitro, GUDCA reduced palmitic acid induced-ER stress and -apoptosis, as well as stabilized calcium homeostasis. In vivo, GUDCA exerted effects on amelioration of HFD-induced insulin resistance and hepatic steatosis. In parallel, ER stress and apoptosis were decreased in GUDCA-treated mice as compared with vehicle-treated mice in liver. These findings demonstrate that reduced GUDCA is an indicator of hyperglycemia. Supplementation of GUDCA could be an option for the treatment of diet-induced metabolic disorders, including insulin resistance and hepatic steatosis, with inhibiting ER stress.
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http://dx.doi.org/10.1042/CS20210198DOI Listing
July 2021

In vitro Activity of Meropenem-Vaborbactam versus Other Antibiotics Against Carbapenem-Resistant from Southeastern China.

Infect Drug Resist 2021 30;14:2499-2507. Epub 2021 Jun 30.

Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035, Zhejiang Province, People's Republic of China.

Purpose: This study aimed to evaluate the in vitro activity of meropenem-vaborbactam (MVB) against a collection of carbapenem-resistant (CREC) isolates and to compare the activity with other antibiotics with regard to different separation sites, carbapenem-resistant mechanisms, and sequence types (STs).

Methods: A total of 58 CREC strains were used as the experimental strains from the First Affiliated Hospital of Wenzhou Medical University in southeastern China. The minimum inhibitory concentrations of MVB, ceftazidime-avibactam, and tigecycline against all the experimental strains were determined by the microdilution broth method.

Results: MVB exhibited higher antimicrobial activity (83% susceptibility) than that of other antibiotics, except for colistin and tigecycline. The susceptibility of CREC strains towards MVB varied with regard to carbapenem-resistant mechanisms and STs, especially in carbapenemase (KPC)-positive isolates and ST8 isolates.

Conclusion: MVB exhibited considerably high activity against KPC-producing and ST8 CREC isolates. It has the great potential to be an alternative for the treatment of infections caused by CREC after determining the type of carbapenemase, the susceptibility to MVB and/or STs.
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http://dx.doi.org/10.2147/IDR.S315384DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255899PMC
June 2021

Construction safety: an analysis of the cross-influence of economic, construction, and accident death factors.

Environ Sci Pollut Res Int 2021 Jul 6. Epub 2021 Jul 6.

School of Management, Wuhan University of Science and Technology, Wuhan, 430081, Hubei, People's Republic of China.

To explore the relations between the number of accident deaths (NCD) and various factors in construction safety, a multiple linear regression model was established, based on the panel data of economy, construction labor, and the number of accident deaths in 31 provinces (regions) of China from 2009 to 2018. The results show that the average total profits and taxes (TPAT), and the death rate of ten thousand people (DR) are positively correlated with NCD, while the labor productivity (LP) is negatively correlated with it. Under the condition that other factors remain unchanged, NCD increases by 0.02 per a hundred million CNY. However, if DR increases by one ten thousand, NCD will increase by 8.66 on average. On the contrary, when LP increases by a hundred million CNY per ten thousand people, NCD decreases by 0.19. The model also predicts NCD in different provinces and cities from 2019 to 2020 with the method of quadratic exponential smoothing. As well, practice results show that the model is effective and practical.
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http://dx.doi.org/10.1007/s11356-021-15231-4DOI Listing
July 2021
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