Dr. Tania Limongi, PhD - King Abdullah University of Science and Technology - Research Scientist

Dr. Tania Limongi

PhD

King Abdullah University of Science and Technology

Research Scientist

Thuwal, Saudi Arabia

Main Specialties: Biology

Additional Specialties: Bioengineering, Regenerative medicine, Advanced microscopies for characterization and micro and nanofabrication

Dr. Tania Limongi, PhD - King Abdullah University of Science and Technology - Research Scientist

Dr. Tania Limongi

PhD

Introduction

Primary Affiliation: King Abdullah University of Science and Technology - Thuwal, Saudi Arabia

Specialties:

Additional Specialties:

Publications

15Publications

157Reads

-Profile Views

50PubMed Central Citations

Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application

IJC Heart & Vasculature , Volume 6 , 48 - 53

IJC Heart & Vasculature

Aim Consistent expansion of primary human endothelial cells in vitro is critical in the development of engineered tissue. A variety of complex culture media and techniques developed from different basal media have been reported with alternate success. Incongruous results are further confounded by donor-to-donor variability and cellular source of derivation. Our results demonstrate how to overcome these limitations using soluble CD54 (sCD54) as additive to conventional culture medium. Methods and results Isolated primary fragment of different vessel types was expanded in Ham's F12 DMEM, enriched with growth factors, Fetal Calf Serum and conditioned medium of Human Umbilical Vein Endothelial Cells (HUVEC) collected at different passages. Cytokine content of culture media was analyzed in order to identify the soluble factors correlating with better proliferation profile. sCD54 was found to induce the in vitro expansion of human endothelial cells (HECs) independently from the vessels source and even in the absence of HUVEC-conditioned medium. The HECs cultivated in the presence of sCD54 (50 ng/ml), resulted positive for the expression of CD146 and negative for CD45, and lower fibroblast contamination. Cells were capable to proliferate with an S phase of 25%, to produce vascular endothelial growth factor, VEGF, (10 ng/ml) and to give origin to vessel-like tubule in vitro. Conclusion Our results demonstrate that sCD54 is an essential factor for the in-vitro expansion of HECs without donor and vessel-source variability. Resulting primary cultures can be useful, for tissue engineering in regenerative medicine (e.g. artificial micro tissue generation, coating artificial heart valve etc.) and bio-nanotechnology applications.

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March 2015
3 Reads

Photolithography and micromolding techniques for the realization of 3D polycaprolactone scaffolds for tissue engineering applications

Microelectronic Engineering 141 (2015) 135–139

Microelectronic Engineering

Material science, cell biology, and engineering are all part of the research field of tissue engineering. It is the application of knowledge, methods and instrumentations of engineering and life science to the development of biocompatible solutions for repair and/or replace tissues and damaged organs. Last generation microfabrication technologies utilizing natural and synthetic biomaterials allow the realization of scaffolds resembling tissue-like structures as skin, brain, bones, muscles, cartilage and blood vessels. In this work we describe an effective and simple micromolding fabrication process allowing the realization of 3D polycaprolactone (PCL) scaffold for human neural stem cells (hNSC) culture. Scanning Electron Microscopy has been used to investigate the micro and nano features characterizing the surface of the device. Immunofluorescence analysis showed how, after seeding cells onto the substrate, healthy astrocytes grew up in a well-organized 3D network. Thus, we proposed this effective fabrication method for the production of nanopatterned PCL pillared scaffold providing a biomimetic environment for the growth of hNSC, a promising and efficient means for future applications in tissue engineering and regenerative medicine.

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February 2015
3 Reads

Development, Characterization and Cell Cultural Response of 3D Biocompatible Micro-Patterned Poly-ε-Caprolactone Scaffolds Designed and Fabricated Integrating Lithography and Micromolding Fabrication Techniques

J Tissue Sci Eng 2015, 6:145

Journal of Tissue Science & Engineering

Scaffold design and fabrication are very important subjects for biomaterial, tissue engineering and regenerative medicine research playing a unique role in tissue regeneration and repair. Among synthetic biomaterials Poly-ε- Caprolactone (PCL) is very attractive bioresorbable polyester due to its high permeability, biodegradability and capacity to be blended with other biopolymers. Thanks to its ability to naturally degrade in tissues, PCL has a great potential as a new material for implantable biomedical micro devices. This work focuses on the establishment of a micro fabrication process, by integrating lithography and micromolding fabrication techniques, for the realization of 3D microstructure PCL devices. Scaffold surface exhibits a combination in the patterned length scale; cylindrical pillars of 10 μm height and 10 μm diameter are arranged in a hexagonal lattice with periodicity of 30 μm and their sidewalls are nano-sculptured, with a regular pattern of grooves leading to a spatial modulation in the z direction. In order to demonstrate that these biocompatible pillared PCL substrates are suitable for a proper cell growth, NIH/3T3 mouse embryonic fibroblasts were seeded on them and cells key adhesion parameters were evaluated. Scanning Electron Microscopy and immunofluorescence analysis were carried out to check cell survival, proliferation and adhesion; cells growing on the PCL substrates appeared healthy and formed a well-developed network in close contact with the micro and nano features of the pillared surface. Those 3D scaffolds could be a promising solution for a wide range of applications within tissue engineering and regenerative medicine applications.

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December 2014
2 Reads

Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells.

Small 2014 Nov 14;10(21):4324-31. Epub 2014 Jul 14.

Italian Institute of Technology, Arnesano, Lecce, Italy; BIONEM, University of Magna Graecia, Campus S. Venuta, Viale Europa, 88100, Catanzaro, Italy.

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http://dx.doi.org/10.1002/smll.201400498DOI Listing
November 2014
18 Reads
2 Citations
8.370 Impact Factor

Probing droplets on superhydrophobic surfaces by synchrotron radiation scattering techniques.

J Synchrotron Radiat 2014 Jul 10;21(Pt 4):643-53. Epub 2014 Jun 10.

European Synchrotron Radiation Facility, BP 220, 38043 Grenoble Cedex, France.

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http://dx.doi.org/10.1107/S1600577514009849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4073955PMC
July 2014
13 Reads
2.740 Impact Factor

Nanostructured superhydrophobic substrates trigger the development of 3D neuronal networks.

Small 2013 Feb 2;9(3):402-12. Epub 2012 Oct 2.

Department of Nanostructures, Istituto Italiano di Tecnologia, Genova, Italy.

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http://dx.doi.org/10.1002/smll.201201377DOI Listing
February 2013
7 Reads
12 Citations
8.370 Impact Factor

Direct imaging of DNA fibers: the visage of double helix.

Nano Lett 2012 Dec 28;12(12):6453-8. Epub 2012 Nov 28.

Nanostructures, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.

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http://dx.doi.org/10.1021/nl3039162DOI Listing
December 2012
4 Reads
6 Citations
13.592 Impact Factor

Identification of a 3-gene model as a powerful diagnostic tool for the recognition of ALK-negative anaplastic large-cell lymphoma.

Blood 2012 Aug 27;120(6):1274-81. Epub 2012 Jun 27.

Department of Clinical Sciences and Community Health, University of Milano, Hematology 1, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Ca' Granda Ospedale Maggiore Policlinico, Milano, Italy.

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http://dx.doi.org/10.1182/blood-2012-01-405555DOI Listing
August 2012
9 Reads
24 Citations
10.452 Impact Factor

Acute mobile phones exposure affects frontal cortex hemodynamics as evidenced by functional near-infrared spectroscopy.

J Cereb Blood Flow Metab 2009 May 25;29(5):903-10. Epub 2009 Feb 25.

Dipartimento di Scienze della Salute, Università di L'Aquila, L'Aquila, Italy.

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http://dx.doi.org/10.1038/jcbfm.2009.14DOI Listing
May 2009
3 Reads
5.410 Impact Factor

Qualitative detection of Mg content in a leaf of Hedera helix by using X-ray radiation from a laser plasma source.

Microsc Res Tech 2008 Jun;71(6):459-68

Università di L'Aquila, Dipartimento di Biologia di Base ed Applicata, Coppito, L'Aquila, Italy.

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http://dx.doi.org/10.1002/jemt.20574DOI Listing
June 2008
17 Reads
1.170 Impact Factor

Current state and perspectives of truffle genetics and sustainable biotechnology.

Appl Microbiol Biotechnol 2006 Sep 27;72(3):437-41. Epub 2006 Jun 27.

Department of Basic and Applied Biology, Faculty of Biotechnologies, University of L'Aquila Via Vetoio, 1-I-67010 Coppito, Italy.

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http://link.springer.com/10.1007/s00253-006-0519-y
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http://dx.doi.org/10.1007/s00253-006-0519-yDOI Listing
September 2006
6 Reads
3 Citations
3.340 Impact Factor

Genotoxicity induced by fine urban air particulate matter in the macrophages cell line RAW 264.7.

Toxicol In Vitro 2006 Sep 28;20(6):1023-9. Epub 2006 Feb 28.

Department of Basic and Applied Biology, Faculty of Sciences, University of L'Aquila, Via Vetoio 1, Localitá Coppito, I-67100 L'Aquila, Italy.

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http://dx.doi.org/10.1016/j.tiv.2006.01.014DOI Listing
September 2006
28 Reads
2 Citations
2.903 Impact Factor

Microradiography as a tool to detect heavy metal uptake in plants for phytoremediation applications.

Microsc Res Tech 2006 Aug;69(8):666-74

Università di L'Aquila, Dipartimento di Fisica and INFM, INFN-LNGS- L'Aquila, Italy.

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http://dx.doi.org/10.1002/jemt.20350DOI Listing
August 2006
32 Reads
1.170 Impact Factor

Tuber borchii mycelial protoplasts isolation, characterization and functional delivery of liposome content, a new step towards truffles biotechnology.

FEMS Microbiol Lett 2005 Dec 24;253(2):331-7. Epub 2005 Oct 24.

Department of Basic and Applied Biology, Faculty of Biotechnology and Faculty of Sciences, University of L'Aquila, Via Vetoio 1, Località Coppito, I-67010 L'Aquila, Italy.

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http://dx.doi.org/10.1016/j.femsle.2005.10.003DOI Listing
December 2005
4 Reads
1 Citation
2.121 Impact Factor

Differences in X-ray absorption due to cadmium treatment in Saponaria officinalis leaves.

Microsc Res Tech 2004 May;64(1):21-9

Università di L'Aquila, Dipartimento di Fisica and INFM,INFN-LNGS, 67010, Coppito L'Aquila, Italy.

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http://dx.doi.org/10.1002/jemt.20041DOI Listing
May 2004
16 Reads
1.170 Impact Factor

Top co-authors

Anna Poma
Anna Poma

University of L'Aquila

6
Antonia Lai
Antonia Lai

Diagnostics and Metrology Laboratory

3
Anatoly Faenov
Anatoly Faenov

Università di L'Aquila

3
Lucia Reale
Lucia Reale

Università di L'Aquila

3
Francesco Flora
Francesco Flora

Università di L'Aquila

3
Antonio Ritucci
Antonio Ritucci

Università di L'Aquila

2
Giovanni Pacioni
Giovanni Pacioni

University of L'Aquila

2
Giuseppe Tomassetti
Giuseppe Tomassetti

Università di L'Aquila

2