Publications by authors named "Takeshi Tanaka"

234 Publications

Estimation of pancreatic fibrosis and prediction of postoperative pancreatic fistula using extracellular volume fraction in multiphasic contrast-enhanced CT.

Eur Radiol 2021 Oct 12. Epub 2021 Oct 12.

Department of Radiology, Kobe University Graduate School of Medicine, 7-5-2, Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan.

Objective: To investigate the diagnostic performance of the extracellular volume (ECV) fraction in multiphasic contrast-enhanced computed tomography (CE-CT) for estimating histologic pancreatic fibrosis and predicting postoperative pancreatic fistula (POPF).

Methods: Eighty-five patients (49 men; mean age, 69 years) who underwent multiphasic CE-CT followed by pancreaticoduodenectomy with pancreaticojejunal anastomosis between January 2012 and December 2018 were retrospectively included. The ECV fraction was calculated from absolute enhancements of the pancreas and aorta between the precontrast and equilibrium-phase images, followed by comparisons among histologic pancreatic fibrosis grades (F0‒F3). The diagnostic performance of the ECV fraction in advanced fibrosis (F2‒F3) was evaluated using receiver operating characteristic curve analysis. Multivariate logistic regression analysis was used to evaluate the associations of the risk of POPF development with patient characteristics, histologic findings, and CT imaging parameters.

Results: The mean ECV fraction of the pancreas was 34.4% ± 9.5, with an excellent intrareader agreement of 0.811 and a moderate positive correlation with pancreatic fibrosis (r = 0.476; p < 0.001). The mean ECV fraction in advanced fibrosis was significantly higher than that in no/mild fibrosis (44.4% ± 10.8 vs. 31.7% ± 6.7; p < 0.001), and the area under the receiver operating characteristic curve for the diagnosis of advanced fibrosis was 0.837. Twenty-two patients (25.9%) developed clinically relevant POPF. Multivariate logistic regression analysis demonstrated that the ECV fraction was a significant predictor of POPF.

Conclusions: The ECV fraction can offer quantitative information for assessing pancreatic fibrosis and POPF after pancreaticojejunal anastomosis.

Key Points: • There was a moderate positive correlation of the extracellular volume (ECV) fraction of the pancreas in contrast-enhanced CT with the histologic grade of pancreatic fibrosis (r = 0.476; p < 0.001). • The ECV fraction was higher in advanced fibrosis (F2‒F3) than in no/mild fibrosis (F0‒F1) (p < 0.001), with an AUC of 0.837 for detecting advanced fibrosis. • The ECV fraction was an independent risk factor for predicting subclinical (odds ratio, 0.81) and clinical (odds ratio, 0.80) postoperative pancreatic fistula.
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http://dx.doi.org/10.1007/s00330-021-08255-4DOI Listing
October 2021

Development and Evaluation of Quantitative Immunoglobulin G Enzyme-Linked Immunosorbent Assay for the Diagnosis of Coronavirus Disease 2019 Using Truncated Recombinant Nucleocapsid Protein as Assay Antigen.

Int J Environ Res Public Health 2021 09 13;18(18). Epub 2021 Sep 13.

Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). Real-time RT-PCR is the most commonly used method for COVID-19 diagnosis. However, serological assays are urgently needed as complementary tools to RT-PCR. Hachim et al. 2020 and Burbelo et al. 2020 demonstrated that anti-nucleocapsid(N) SARS-CoV-2 antibodies are higher and appear earlier than the spike antibodies. Additionally, cross-reactive antibodies against N protein are more prevalent than those against spike protein. We developed a less cross-reactive immunoglobulin G (IgG) indirect ELISA by using a truncated recombinant SARS-CoV-2 N protein as assay antigen. A highly conserved region of coronaviruses N protein was deleted and the protein was prepared using an protein expression system. A total of 177 samples collected from COVID-19 suspected cases and 155 negative control sera collected during the pre-COVID-19 period were applied to evaluate the assay's performance, with the plaque reduction neutralization test and the commercial SARS-CoV-2 spike protein IgG ELISA as gold standards. The SARS-CoV-2 N truncated protein-based ELISA showed similar sensitivity (91.1% vs. 91.9%) and specificity (93.8% vs. 93.8%) between the PRNT and spike IgG ELISA, as well as also higher specificity compared to the full-length N protein (93.8% vs. 89.9%). Our ELISA can be used for the diagnosis and surveillance of COVID-19.
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http://dx.doi.org/10.3390/ijerph18189630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469721PMC
September 2021

Epidemiology of Coronavirus Disease Outbreak among Crewmembers on Cruise Ship, Nagasaki City, Japan, April 2020.

Emerg Infect Dis 2021 09;27(9):2251-2260

In April 2020, a coronavirus disease (COVID-19) outbreak occurred on the cruise ship Costa Atlantica in Nagasaki, Japan. Our outbreak investigation included 623 multinational crewmembers onboard on April 20. Median age was 31 years; 84% were men. Each crewmember was isolated or quarantined in a single room inside the ship, and monitoring of health status was supported by a remote health monitoring system. Crewmembers with more severe illness were hospitalized. The investigation found that the outbreak started in late March and peaked in late April, resulting in 149 laboratory-confirmed and 107 probable cases of infection with severe acute respiratory syndrome coronavirus 2. Six case-patients were hospitalized for COVID-19 pneumonia, including 1 in severe condition and 2 who required oxygen administration, but no deaths occurred. Although the virus can spread rapidly on a cruise ship, we describe how prompt isolation and quarantine combined with a sensitive syndromic surveillance system can control a COVID-19 outbreak.
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http://dx.doi.org/10.3201/eid2709.204596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386778PMC
September 2021

Nationwide surveillance of AIDS-defining illnesses among HIV patients in Japan from 1995 to 2017.

PLoS One 2021 19;16(8):e0256452. Epub 2021 Aug 19.

Infection Control and Education Center, Nagasaki University Hospital, Nagasaki-shi, Nagasaki, Japan.

Objectives: The accurate prevalence of acquired immunodeficiency syndrome (AIDS)-defining illnesses (ADIs) in human immunodeficiency virus (HIV)-infected patients has not been well investigated. Hence, a longitudinal nationwide surveillance study analyzing the current status and national trend of opportunistic complications in HIV-infected patients in Japan is warranted.

Methods: A nationwide surveillance of opportunistic complications in HIV-infected patients from 1995 to 2017 in Japan was conducted. An annual questionnaire was sent to 383 HIV/AIDS referral hospitals across Japan to collect information (CD4+ lymphocyte count, time of onset, outcome, and antiretroviral therapy [ART] status) of patients diagnosed with any of 23 ADIs between 1995 and 2017.

Results: The response and case capture rates of the questionnaires in 2017 were 53% and 76%, respectively. The number of reported cases of opportunistic complications peaked in 2011 and subsequently declined. Pneumocystis pneumonia (38.7%), cytomegalovirus infection (13.6%), and candidiasis (12.8%) were associated with the cumulative incidence of ADIs between 1995 and 2017. The mortality rate in HIV-infected patients with opportunistic complications substantially decreased to 3.6% in 2017. The mortality rate was significantly higher in HIV patients who received ART within 14 days of diagnosis of complications than in those who received ART 15 days after diagnosis (13.0% vs. 3.2%, p < 0.01).

Conclusions: We have demonstrated a 23-year trend of a newly diagnosed AIDS status in Japan with high accuracy. The current data reveal the importance of Pneumocystis pneumonia as a first-onset illness and that early initiation of ART results in poor outcomes in HIV patients in Japan.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0256452PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376045PMC
August 2021

Discrepancy of SARS-CoV-2 PCR results due to the sample collection sites and possible improper sampling.

J Infect Chemother 2021 Oct 16;27(10):1525-1528. Epub 2021 Jul 16.

Department of Respiratory Medicine, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.

Polymerase chain reaction (PCR) testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is necessary for confirming a diagnosis of Coronavirus disease 2019 (COVID-19). Here we present a COVID-19 case of an elderly woman whose SARS-CoV-2 PCR tests showed false negative repeatedly by evaluating with different sampling sites and procedures. Nasopharyngeal swabs, suctioned sputum, and tongue swabs were collected for SARS-CoV-2-PCR. As for tongue swabs, we compared between two different sample conditions; one obtained with dry condition and the other obtained with moistened condition inside the oral cavity. SARS-CoV-2-PCR showed positive for an extended period with suctioned sputum samples compared with nasopharyngeal swabs and tongue swabs. No SARS-CoV-2 from a nasopharyngeal swab sample obtained on day 46 after symptoms onset was isolated despite high viral load (183740.5 copies/5μL). An adequate production of neutralizing antibody in a serum sample on day 46 was also confirmed. The number of RNA copies of the tongue swab samples was higher with moistened condition than with dry condition. The present case suggests that the difference of sampling site or sample condition can affect PCR results. High loads viral RNA detection does not always correlate with infectivity.
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http://dx.doi.org/10.1016/j.jiac.2021.07.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282446PMC
October 2021

Impact of plasma 5-hydroxyindoleacetic acid, a serotonin metabolite, on clinical outcome in septic shock, and its effect on vascular permeability.

Sci Rep 2021 07 8;11(1):14146. Epub 2021 Jul 8.

Infection Control and Education Center, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.

Septic shock is characterized by dysregulated vascular permeability. We hypothesized that the vascular permeability of endothelial cells (ECs) would be regulated by serotonin via serotonin-Rho-associated kinase (ROCK) signaling. We aimed to determine the impact of 5-hydroxyindoleacetic acid (5-HIAA) on septic shock as a novel biomarker. Plasma 5-HIAA levels and disease severity indices were obtained from 47 patients with sepsis. The association between 5-HIAA levels and severity indices was analyzed. Permeability upon serotonin stimulation was determined using human pulmonary microvascular ECs. 5-HIAA were significantly higher in septic shock patients than in patients without shock or healthy controls (p = 0.004). These elevated levels were correlated with severity indexes (SOFA score [p < 0.001], APACHE II [p < 0.001], and PaO:FiO [p = 0.02]), and longitudinally associated with worse clinical outcomes (mechanical ventilation duration [p = 0.009] and ICU duration [p = 0.01]). In the experiment, serotonin increased the permeability of ECs, which was inhibited by the ROCK inhibitor (p < 0.001). Serotonin increases vascular permeability of ECs via ROCK signaling. This suggests a novel mechanism by which serotonin disrupts endothelial barriers via ROCK signaling and causes the pathogenesis of septic shock with a vascular leak. Serotonin serves as a novel biomarker of vascular permeability.
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http://dx.doi.org/10.1038/s41598-021-93649-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266895PMC
July 2021

Detection of SARS-CoV-2 using qRT-PCR in saliva obtained from asymptomatic or mild COVID-19 patients, comparative analysis with matched nasopharyngeal samples.

PLoS One 2021 10;16(6):e0252964. Epub 2021 Jun 10.

Nagasaki University, Nagasaki, Japan.

Objectives: The accurate detection of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is essential for the diagnosis of coronavirus disease 2019 (COVID-19). We compared the quantitative RT-PCR results between nasopharyngeal swabs and saliva specimens.

Methods: A COVID-19 outbreak occurred on a cruise ship at Nagasaki port, Japan. We obtained 123 nasopharyngeal swabs and saliva each from asymptomatic or mild patients in the late phase of infection.

Results: The intervals from the diagnosis to the sampling were 25.5 days for nasopharyngeal swabs and 28.9 days for saliva. The positive rate was 19.5% (24/123) for nasopharyngeal swabs and 38.2% (47/123) for saliva (P = 0.48). The quantified viral copies (mean ± SEM copies/5 μl) were 9.3±2.6 in nasopharyngeal swabs and 920±850 in saliva (P = 0.0006).

Conclusions: The advantages of saliva specimens include positive rate improvement and accurate viral load detection. Saliva may be used as a reliable sample for SARS-CoV-2 detection.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252964PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191987PMC
June 2021

Evaluation of four commercial severe acute respiratory coronavirus 2 antibody tests.

J Infect Chemother 2021 Jul 15;27(7):1033-1038. Epub 2021 Mar 15.

Department of Respiratory Medicine, Nagasaki University Hospital, Nagasaki, Japan.

Introduction: Numerous severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serological tests exists commercially; however, their performance using clinical samples is limited. Although insufficient to detect SARS-CoV-2 in the early phase of infection, antibody assays can be of great use for surveillance studies or for some coronavirus disease 2019 (COVID-19) patients presenting late to the hospital.

Methods: This study evaluated the sensitivity and specificity of four commercial SARS-CoV-2 lateral flow antibody tests using 213 serum specimens from 90 PCR-positive confirmed COVID-19 patients. Of 59 negative control sera, 50 were obtained from patients with other respiratory infectious diseases before COVID-19 pandemic began while nine were from patients infected with other respiratory viruses, including two seasonal coronaviruses.

Results: The varied sensitivities for the four commercial kits were 70.9%, 65.3%, 45.1%, and 65.7% for BioMedomics, Autobio Diagnostics, Genbody, and KURABO, respectively, between sick days 1 and 155 in COVID-19 patients. The sensitivities of the four tests gradually increased over time after infection before sick day 5 (15.0%, 12.5%, 15.0%, and 20.0%); from sick day 11-15 (95.7%, 87.2%, 53.2%, and 89.4%); and after sick day 20 (100%, 100%, 68.6%, and 96.1%), respectively. For severe illness, the sensitivities were quite high in the late phase after sick day 15. The specificities were over 96% for all four tests. No cross-reaction due to other pathogens, including seasonal coronaviruses, was observed.

Conclusions: Our results demonstrated the large differences in the antibody test performances. This ought to be considered when performing surveillance analysis.
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http://dx.doi.org/10.1016/j.jiac.2021.03.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959261PMC
July 2021

Author Correction: Cigarette smoke induces endoplasmic reticulum stress and suppresses efferocytosis through the activation of RhoA.

Sci Rep 2021 Mar 8;11(1):5866. Epub 2021 Mar 8.

Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki City, Nagasaki, 852-8523, Japan.

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http://dx.doi.org/10.1038/s41598-021-85556-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940424PMC
March 2021

A unique missense variant in the E1A-binding protein P400 gene is implicated in schizophrenia by whole-exome sequencing and mutant mouse models.

Transl Psychiatry 2021 02 18;11(1):132. Epub 2021 Feb 18.

Department of Neuropsychiatry, Unit of Translation Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

Genetic and epidemiological evidence has suggested that genetic factors are important in schizophrenia, although its pathophysiology is poorly understood. This study used whole-exome sequencing to investigate potential novel schizophrenia-causing genes in a Japanese family containing several members affected by severe or treatment-resistant schizophrenia. A missense variant, chr12:132064747C>T (rs200626129, P2805L), in the E1A-binding protein P400 (EP400) gene completely segregated with schizophrenia in this family. Furthermore, numerous other EP400 mutations were identified in the targeted sequencing of a schizophrenia patient cohort. We also created two lines of Ep400 gene-edited mice, which had anxiety-like behaviours and reduced axon diameters. Our findings suggest that rs200626129 in EP400 is likely to cause schizophrenia in this Japanese family, and may lead to a better understanding and treatment of schizophrenia.
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http://dx.doi.org/10.1038/s41398-021-01258-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892873PMC
February 2021

Safety and feasibility of lung biopsy in diagnosis of acute respiratory distress syndrome: protocol for a systematic review and meta-analysis.

BMJ Open 2021 02 12;11(2):e043600. Epub 2021 Feb 12.

Primary Care and Emergency Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Introduction: Acute respiratory distress syndrome (ARDS) is a type of acute respiratory failure characterised by non-cardiac pulmonary oedema caused by various underlying conditions. ARDS is often pathologically characterised by diffuse alveolar damage, and its pathological findings have been reported to be associated with prognosis, although the adverse effects of lung biopsies to obtain pathological findings are still unclear. The purpose of this systematic review and meta-analysis is to reveal the safety and feasibility of lung biopsy in the diagnosis of ARDS.

Methods And Analysis: We will include studies that were published in MEDLINE and Cochrane Central Register of Controlled Trials until 1 June 2020. We will include the reports for critically ill patients in an intensive care unit or emergency department who undergo lung biopsy and require a mechanical ventilation. Two review authors will independently scan titles and abstracts of all identified studies. Furthermore, these two authors will read and assess the full text of study reports to identify trials that appeared broadly to address the subject of the review. We will perform a risk of bias assessment using the McMaster Quality Assessment Scale of Harms.

Ethics And Dissemination: This study will be based on the published data, therefore, it does not require ethical approval. The final results of the study will be published in a peer-reviewed journal.

Trial Registration Number: UMIN000040650.
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http://dx.doi.org/10.1136/bmjopen-2020-043600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883844PMC
February 2021

Lanthanide Luminescence Enhancement of Core-Shell Magnetite-SiO Nanoparticles Covered with Chain-Structured Helical Eu/Tb Complexes.

ACS Omega 2020 Dec 16;5(51):32930-32938. Epub 2020 Dec 16.

Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.

Oligomeric-brush chains of helical lanthanide (Ln) complexes retain their structural and luminescent behavior after coating onto magnetic nanoparticles (MNPs) consisting of FeO covered with silicate. It is one of the type of bifunctional NPs exhibiting luminescence of Ln and superparamagnetism of FeO. In comparison to a simple monolayer of complexes adsorbed on a modified surface, a layer made of luminescent chains allowed us to obtain a more intensive red/green luminescence originating from Eu/Tb ions, and at the same time, no visible increase in particle size (compared to [email protected] particles) was observed. The luminescent properties of the Tb complex were altered by MNPs; the decrease of the luminescence was not as large as expected, the excitation spectrum changed significantly, and the average luminescence lifetime was much longer at room temperature. Surprisingly, this phenomenon was not observed at 77 K and also did not occur for the Eu complexes. The possibility to stack building blocks in a chain using complexes of different lanthanide ions can be used to design novel multifunctional nanosystems.
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http://dx.doi.org/10.1021/acsomega.0c03746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774089PMC
December 2020

Acute pancreatitis in intraductal papillary mucinous neoplasms correlates with pancreatic volume and epithelial subtypes.

Pancreatology 2021 Jan 20;21(1):138-143. Epub 2020 Nov 20.

Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.

Background: Intraductal papillary mucinous neoplasm (IPMN) of the pancreas is associated with acute pancreatitis (AP) in some cases, however its causes have not been fully elucidated. We investigated the association of the incidence of AP with epithelial subtypes and pancreatic volume in IPMN.

Methods: This retrospective study included 182 consecutive surgically resected IPMN patients between January 2000 and December 2018. The relationship between the incidence of AP and epithelial subtypes of IPMN and pancreatic volume was investigated. Epithelial subtypes of IPMN were classified into gastric (G type: N = 116), intestinal (I type: N = 49), pancreatobiliary (PB type: N = 14), and oncocytic types (O type: N = 3). Pancreatic volume of the contrast-enhanced computed tomography scan was measured using Ziostation2 software. Histological pancreatic parenchymal atrophy was also evaluated.

Results: AP occurred more frequently in I-types (I-type vs. G-type, 22.4% [11/49] vs 3.4% [4/116], P = 0.003) and PB-types (PB type vs. G-type, 35.7% [5/14] vs. 3.4% [4/116], P = 0.007) in comparison with G-types, which constituted the majority of the resected IPMNs. AP occurred more frequently in I-type patients with high pancreatic volumes (I-type with high pancreatic volume vs. I-type with low pancreatic volume, 37.0% [10/27] vs. 4.7% [1/21], P = 0.02). However, histological atrophy did not show an additional influence on the association between the incidence of AP and epithelial subtypes. The elevation of serum pancreatic enzymes was not significantly related to epithelial subtypes.

Conclusion: Epithelial subtypes and the degree of pancreatic volume may be closely associated with the incidence of AP in IPMN.
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http://dx.doi.org/10.1016/j.pan.2020.11.005DOI Listing
January 2021

Pneumatocele formation in a fatal adult pneumonia patient coinfected with Streptococcus pyogenes emm-type 3 and influenza A: a case report.

BMC Infect Dis 2020 Nov 26;20(1):892. Epub 2020 Nov 26.

Department of Infectious Diseases, Nagasaki University Hospital, 1-7-1, Sakamoto, Nagasaki, 852-8501, Japan.

Background: A pneumatocele is a transient thin-walled lesion and rare complication in adult pneumonia. A variety of infectious pathogens have been reported in children with pneumatoceles. We report the first case of adult pneumonia with pneumatocele formation that is likely caused by Streptococcus pyogenes and coinfection with influenza A virus.

Case Presentation: A 64-year-old Japanese man presented with a one-week history of fever, sore throat, and arthralgia. He was referred to our university hospital for respiratory distress. He required mechanical ventilation in the intensive care unit (ICU). Bacterial culture detected S. pyogenes in the bronchoscopic aspirates, which was not detected in blood. Although a rapid influenza antigen test was negative, an influenza A polymerase chain reaction (PCR) test was positive. Therefore, he was diagnosed with coinfection of influenza A and group A streptococcus (GAS) pneumonia complicated by probable streptococcal toxic shock syndrome. A chest radiograph on admission showed diffuse patchy opacification and consolidation in the bilateral lung fields. Multiple thin-walled cysts appeared in both middle lung fields on computed tomography (CT). On the following day, the bilateral cysts had turned into a mass-like opacity. The patient died despite intensive care. An autopsy was performed. The pathology investigation revealed multiple hematomas formed by bleeding in pneumatoceles.

Conclusions: There have been no previous reports of a pneumatocele complicated by S. pyogenes in an adult patient coinfected with influenza A. Further molecular investigation revealed that the S. pyogenes isolate had the sequence type of emm3.
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http://dx.doi.org/10.1186/s12879-020-05595-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688446PMC
November 2020

Crystal structure of bis-[μ--(η-prop-2-en-1-yl)piperidine-1-carbo-thio-amide-κ :]bis-[(thio-cyanato-κ)copper(I)].

Acta Crystallogr E Crystallogr Commun 2020 Nov 6;76(Pt 11):1712-1715. Epub 2020 Oct 6.

Osaka Research Institute of Industrial Science and Technology, 2-7-1 Ayumino, Izumi, Osaka 594-1157, Japan.

The title crystalline compound, [Cu(NCS)(CHN)], was obtained from the reaction of copper(I) thio-cyanate (CuSCN) with (-prop-2-en-1-yl)piperidine-1-carbo-thio-amide as a chelating and bridging thio-urea ligand in chloro-benzene. The CuS core of the dimeric mol-ecule is situated on a crystallographic inversion centre. The copper atom is coordinated by a thio-cyanate nitro-gen atom, each sulfur atom of the two thio-urea ligands, and the C=C double bond of the ligand in a distorted tetra-hedral geometry. The dimers are linked by N-H⋯S hydrogen bonds, forming a network extending in two dimensions parallel to (100).
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http://dx.doi.org/10.1107/S2056989020013146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643236PMC
November 2020

An autopsy case of severe acute pancreatitis induced by administration of pazopanib following nivolumab.

Pancreatology 2021 Jan 5;21(1):21-24. Epub 2020 Nov 5.

Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.

Drug-induced pancreatitis is often mild to moderate in severity, but severe and even fatal cases can occur. Here, we report a 74-year-old woman undergoing chemotherapy for recurrent renal cell carcinoma, who presented with abdominal pain after administration of pazopanib following nivolumab and was diagnosed with severe acute pancreatitis. Administration of methylprednisolone and conservative treatment were initiated, but clinical findings and laboratory tests rapidly worsened. When she died, an autopsy was performed. The autopsy findings suggested the possibility of pancreatitis as immune-related adverse events. To the best of our knowledge, no fatal cases of acute pancreatitis due to nivolumab or pazopanib have been reported. We considered that the effects of nivolumab were sustained in the pancreas, and pazopanib administration might have worsened the toxicity.
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http://dx.doi.org/10.1016/j.pan.2020.11.002DOI Listing
January 2021

Visualizing Worklog Based on Human Working Activity Recognition Using Unsupervised Activity Pattern Encoding.

Annu Int Conf IEEE Eng Med Biol Soc 2020 07;2020:4165-4168

Wearable motion sensor-based complex activity recognition during working hours has recently been studied to evaluate and thereby improve worker productivity. In the application of this technique to practical fields, one of the biggest challenges is performing time-consuming modeling tasks such as data labeling and hand-crafted feature extraction. One way to enable faster modeling is to decrease the time required for the manual tasks by making use of unlabeled motion datasets and the characteristics of complex activities. In this study, we propose a working activity recognition method that combines unsupervised encoding of the activity patterns of motions (denoted as "atomic activities"), the representation of working activities by combination of atomic activities, and the integration of additional information such as sensor time. We evaluated our method using an actual dataset from the caregiving field and found that it had an equivalent recognition performance (70.3% macro F-measure) to conventional hand-crafted feature extraction method. This is also comparable to that of previous methods using large labeled datasets. We also found that our method could visualize daily work processes with the accuracy of 71.2%. These results indicate that the proposed method has the potential to contribute to the rapid implementation of working activity recognition in actual working fields.
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http://dx.doi.org/10.1109/EMBC44109.2020.9176710DOI Listing
July 2020

Quantitative analysis of the effect of reabsorption on the Raman spectroscopy of distinct (, ) carbon nanotubes.

Anal Methods 2020 05;12(18):2376-2384

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

We quantitatively analyze the effect of reabsorption on the Raman spectroscopy of (10, 3) and (8, 3) single-chirality single-wall carbon nanotube (SWCNT) solutions by varying the detection depth in confocal micro-Raman measurements and SWCNT concentration the in sample solution. The increase of the detection depth and concentration of SWCNTs enhances the reabsorption effect and decreases the intensities of the Raman features. More importantly, reabsorption exhibits different effects on different Raman features such as the radial breathing mode (RBM) and G+ band, strongly depending on the resonance degree of the scattered light energy and the interband transition of SWCNTs. When (10, 3) SWCNTs are excited with a 633 nm laser, the scattered light from RBM has stronger resonance with the interband transition of the SWCNTs than that from the G+ band, leading to a faster reduction in the RBM intensity and a lower intensity ratio of RBM to the G+ band. In contrast, when (8, 3) SWCNTs are excited with a 633 nm laser, reabsorption has the same effect on the RBM and G+ band intensities and thus maintains a constant intensity ratio of RBM to the G+ band. Furthermore, we precisely establish a quantitative relationship of the intensities of the Raman features such as RBM, the G+ band and their intensity ratio as a function of the focal depth and SWCNT concentration by theoretical calculations and numerical simulation, which reproduces the experimental results well. These results are very useful in the precise analysis of the Raman spectroscopy of SWCNTs and thus their applications in molecular detection and imaging.
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http://dx.doi.org/10.1039/d0ay00356eDOI Listing
May 2020

Relationship between truck driver fatigue and rear-end collision risk.

PLoS One 2020 11;15(9):e0238738. Epub 2020 Sep 11.

Laboratory for Pathophysiological and Health Science, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo, Japan.

The fatigue of truck, bus, and taxi drivers has been a causal trigger for road accidents. However, the relationship between collision risk and the extent of objective fatigue has yet to be confirmed. In this study, we aimed to identify the relationship between autonomic nerve function as an objective parameter of fatigue and the extent of rear-end collision risk, which includes not only objectively risky events but also situations in which truck drivers require safety guidance from safety transport managers. Data of 33 truck driver participants (2 females, 31 males, 46.0 ± 9.1 years old, min-max: 24-65 years old) were analyzed. Drive recorder and automotive sensor data were collected over an eight-month period, and the autonomic nerve function during resting state in drivers was evaluated daily, pre- and post-shift, using pulse waves and electrocardiographic waveform measurement. The rear-end collision risk Index was developed using decision tree analysis of the audiovisual drive recorder data and distance data from the front automotive sensors. The rear-end collision risk index of shift-day was positively correlated with the sympathetic nerve activity index of post-shift condition on the previous day. This suggests that fatigue-related sympathetic nerve overactivity of post-shift condition increases the rear-end collision risk in the following day. Measures, such as actively seeking rest and undertaking fatigue recovery according to the degree of sympathetic nerve activity of post-shift condition, are necessary in order to prevent truck drivers' rear-end collisions.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238738PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485791PMC
November 2020

A case of high-grade pancreatic intraepithelial neoplasia diagnosed based on focal pancreatic parenchymal atrophy after acute pancreatitis.

Clin J Gastroenterol 2020 Dec 17;13(6):1338-1342. Epub 2020 Aug 17.

Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan.

A 60-year-old male visited a previous hospital with upper abdominal pain. He was diagnosed with localized mild acute pancreatitis. Three months later, abdominal contrast-enhanced computed tomography showed focal parenchymal atrophy of the pancreas with distal pancreatic duct dilation. No obvious solid mass could be found at the site of the pancreatic duct stenosis on imaging examinations. Endoscopic retrograde pancreatography showed focal mild stenosis with distal pancreatic duct dilation in the tail of the pancreas. Carcinoma in situ of the pancreas was strongly suspected, especially based on the presence of focal atrophy of the pancreas around the site of stenosis of the main pancreatic duct and the distal pancreatic duct dilation. Laparoscopic distal pancreatectomy was performed. Histologically, high-grade pancreatic intraepithelial neoplasia was found in the epithelium of the stenotic main pancreatic duct and its branches. This case suggests that localized acute pancreatitis and focal atrophy of the pancreas with distal dilation of the pancreatic duct could be important clinical manifestations of pancreatic carcinoma in situ.
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http://dx.doi.org/10.1007/s12328-020-01208-2DOI Listing
December 2020

Prediction of pancreatic atrophy after steroid therapy using equilibrium-phase contrast computed tomography imaging in autoimmune pancreatitis.

JGH Open 2020 Aug 10;4(4):677-683. Epub 2020 Mar 10.

Division of Gastroenterology, Department of Internal Medicine Kobe University Graduate School of Medicine Kobe Japan.

Background And Aims: Imaging tools for predicting pancreatic atrophy after steroid therapy in autoimmune pancreatitis (AIP) have not been established. As delayed equilibrium-phase contrast enhancement in computed tomography (CE-CT) may reflect interstitial fibrosis, we evaluated the ability of equilibrium-phase CT imaging for predicting pancreatic atrophy.

Methods: Forty-six steroid-treated AIP patients who underwent contrast-enhanced CT at our university hospital were included in this retrospective study. CT attenuation (Hounsfield units [HU]) values in noncontrast images (NC) and equilibrium-phase images (EP) and the differences in HU values between NC and EP images (SUB) were measured. Pancreatic volume was measured in CE-CT before (Vol) and after (Vol) steroid therapy. The volume reduction rate was calculated. The relationships of CT values with pancreatic atrophy, Vol, volume reduction rate, and diabetes exacerbation were investigated.

Results: CT values in the EP and SUB images before steroid therapy were associated with pancreatic atrophy after steroid therapy (atrophy nonatrophy 114.5 ± 12.8 99.5 ± 11.1, = 0.0002; 70.9 ± 14.72 57.2 ± 13.1, = 0.003, respectively), but CT values in NC images were not ( = 0.42). CT values in EP and SUB images before steroid therapy were correlated with Vol (EP images = -0.70, = 0.002; SUB images = -0.68, = 0.03) and volume reduction rate after steroid therapy (EP images: = -0.55, < 0.0001; SUB images = -0.45, = 0.002). Diabetes exacerbation was associated with higher EP and SUB values ( = 0.009 and = 0.04, respectively).

Conclusion: Equilibrium-phase contrast CT imaging may facilitate prediction of pancreatic atrophy after steroid therapy in AIP.
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http://dx.doi.org/10.1002/jgh3.12316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411657PMC
August 2020

Cigarette smoke induces endoplasmic reticulum stress and suppresses efferocytosis through the activation of RhoA.

Sci Rep 2020 07 28;10(1):12620. Epub 2020 Jul 28.

Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki City, Nagasaki, 852-8523, Japan.

Impaired efferocytosis is a key mechanism of inflammatory lung diseases, including chronic obstructive pulmonary disease and cystic fibrosis. Cigarette smoking activates RhoA and impairs efferocytosis in alveolar macrophages, but the mechanism has not been fully elucidated. We investigated the role of endoplasmic reticulum (ER) stress induced by cigarette smoking in the disruption of efferocytosis. Both tunicamycin (10 μg/ml) and thapsigargin (0.1 and 1 μM), which are ER stress inducers, suppressed efferocytosis in J774 cells, and a Rho-associated coiled-coil-forming kinase (ROCK) inhibitor (Y27632) reversed this effect. We validated the effect of tunicamycin on efferocytosis in experiments using RAW264.7 cells. Then, we investigated the role of the unfolded protein response (UPR) in efferocytosis impaired by ER stress. A PERK inhibitor (GSK2606414) restored the efferocytosis that had been impaired by TM, and an eIF2α dephosphorylation inhibitor (salubrinal) suppressed efferocytosis. Cigarette smoke extract (CSE) induced ER stress in J774 macrophages and RhoA activation in J774 cells, and the CSE-induced ROCK activity was successfully reversed by GSK2606414 and tauroursodeoxycholic acid. Finally, we confirmed that ER stress suppresses efferocytosis in murine alveolar macrophages and that GSK2606414 could rescue this process. These data suggest that cigarette smoke-induced ER stress and the UPR play crucial roles in RhoA activation and suppression of efferocytosis in the lung.
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http://dx.doi.org/10.1038/s41598-020-69610-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387437PMC
July 2020

A Case in which an Intraductal Papillary Neoplasm of the Bile Duct Was Surgically Resected 12 Years after the Initial Diagnosis.

Intern Med 2020 Nov 21;59(22):2879-2883. Epub 2020 Jul 21.

Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.

A 66-year-old Japanese man was referred to our hospital with multiple giant liver cysts. The cysts had already been detected as multiple 3-cm cysts with small nodules at another hospital 12 years prior to this presentation. The cysts were diagnosed as an intraductal papillary neoplasms of the bile duct (IPNB) occupying the right lobe of the liver. Extended right lobectomy was performed. Based on the pathological findings, the tumor was diagnosed to be an oncocytic-type IPNB with minimal invasion. This experience suggests that the progression of IPNBs occur relatively slowly. The present case might provide important information for understanding the natural history of IPNBs.
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http://dx.doi.org/10.2169/internalmedicine.4891-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7725632PMC
November 2020

Skin thickness in neonates: Implications for intradermal vaccination.

Vaccine 2020 07 10;38(35):5659-5664. Epub 2020 Jul 10.

Department of Pediatrics, Saiseikai Niigata Hospital, Niigata, Japan.

Introduction: Intradermal (ID) injection is an alternate route that enhances vaccine immunogenicity and decreases vaccine dose. Regular immunization usually starts at age 2 months, and the limited immune capacity of neonates and young infants makes them vulnerable to infection. Successful ID vaccine delivery in this population requires knowledge of skin thickness. Although skin thickness has been evaluated in infants aged 2 months or older, no comparable data are available for neonates, including preterm neonates.

Methods: This prospective observational study used ultrasonography to assess skin thickness in 70 neonates (35 full-term and 35 preterm neonates) at deltoid, suprascapular, and thigh sites. The measurements were compared in relation to anatomical site, between full-term and preterm infants, and with skin thickness values for children aged 2 months or older, which were collected in our previous study using the same measurement technique.

Results: In full-term neonates, skin was significantly thicker at the suprascapular site than at the deltoid and thigh sites (P < 0.05); in preterm neonates, skin was significantly thicker at the suprascapular site than at the thigh site (P < 0.05). Skin thickness values at all three sites were significantly lower in preterm neonates than in full-term neonates (P < 0.05). As compared with skin thickness values for infants aged 2 months, values for full-term neonates were significantly lower for the deltoid and suprascapular sites (P < 0.001).

Conclusions: Skin thickness values for neonates were affected by prematurity and were significantly lower than those for infants aged 2 months. These findings are important in the design of ID injection devices for neonates and young infants.
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http://dx.doi.org/10.1016/j.vaccine.2020.06.061DOI Listing
July 2020

Cascade Reaction-Based Chemiresistive Array for Ethylene Sensing.

ACS Sens 2020 05 11;5(5):1405-1410. Epub 2020 May 11.

Interdisciplinary Research Center for Catalytic Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8565, Japan.

Chemiresistive sensors, which are based on semiconducting materials, offer real-time monitoring of environment. However, detection of nonpolar chemical substances is often challenging because of the weakness of the doping effect. Herein, we report a concept of combining a cascade reaction (CR) and a chemiresistive sensor array for sensitive and selective detection of a target analyte (herein, ethylene in air). Ethylene was converted to acetaldehyde through a Pd-catalyzed heterogeneous Wacker reaction at 40 °C, followed by condensation with hydroxylamine hydrochloride to emit HCl vapor. HCl works as a strong dopant for single-walled carbon nanotubes (SWCNTs), enabling the to detect ethylene with excellent sensitivity (10.9% ppm) and limit of detection (0.2 ppm) in 5 min. False responses that occur in the are easily discriminated by that partially employ CR. Moreover, though the sensor monitors the variation of normalized electric resistance (Δ/) in the SWCNT network, temporary deactivation of CR yields a sensor system that does not require analyte-free air for a baseline correction (i.e., estimation of ) and recovery of response. The concept presented here is generally applicable and offers a solution for several issues that are inherently present in chemiresistive sensing systems.
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http://dx.doi.org/10.1021/acssensors.0c00194DOI Listing
May 2020

Validation of a new serum granulocyte-macrophage colony-stimulating factor autoantibody testing kit.

ERJ Open Res 2020 Jan 27;6(1). Epub 2020 Jan 27.

National Hospital Organization Kinki-Chuo Chest Medical Center, Osaka, Japan.

Very recently, a modest but significant efficacy of granulocyte-macrophage colony-stimulating factor (GM-CSF) inhalation therapy for the treatment of mild to moderate autoimmune pulmonary alveolar proteinosis (aPAP) has been reported. As the ability to measure the level of GM-CSF autoantibody (GMAb) in the serum is required to decide the indication for this therapy, we developed a high-performance GMAb testing kit for clinical use. As the kit succeeded in reducing nonspecific IgG binding to the ELISA plate, the predictive performance shown in the training study to discriminate aPAP patients from healthy subjects was perfect, providing a cut-off value of 1.65 U·mL in 78 patients with aPAP and 90 healthy subjects in an operator-blinded manner using logistic regression analysis. As in the validation study, serum samples from another 213 patients with aPAP were also blinded and evaluated in an operator-blinded manner against external 207 samples from patients with other types of PAP and patients exhibiting various ground-glass opacities on chest high-resolution computed tomography that require discrimination from PAP. The logistic regression analysis of these validation data sets revealed values of 97.6% and 100% for specificity and sensitivity, respectively. Thus, this new GMAb testing kit is reliable for the diagnosis of aPAP and differential diagnosis of other lung diseases.
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http://dx.doi.org/10.1183/23120541.00259-2019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983497PMC
January 2020

Photoluminescence Quantum Yield of Single-Wall Carbon Nanotubes Corrected for the Photon Reabsorption Effect.

Nano Lett 2020 Jan 26;20(1):410-417. Epub 2019 Dec 26.

Nanomaterials Research Institute , National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Ibaraki 305-8565 , Japan.

Photoluminescence (PL) from single-wall carbon nanotubes (SWCNTs) enables structural identification, but to derive the content rate of the specific chirality species it is necessary to know the quantum yield of each chirality. However, in the PL of SWCNTs, because the Stokes shift is small, the photon reabsorption effect is dominant and the apparent PL spectral shape and emission intensity are greatly modified depending on the concentration. This problem makes quantitative identification of SWCNTs by PL difficult. In this study, the concentration dependence of the PL of SWCNTs separated into a few chiralities was analyzed in detail, including the effect of reabsorption. It is clear that all changes in the PL spectrum occurring in the high concentration range can be explained simply by the reabsorption effect, and additional effects such as Coulomb interactions between SWCNTs can be negligible. Furthermore, a reliable quantum yield was derived from the emission intensity corrected for the reabsorption effect. The PL quantum yield varied with SWCNT chirality and exhibited a clear "family pattern". This is consistent with the theoretical report showing that the chirality-dependent PL quantum yield is dominated mainly by relaxation by optical phonons from E to E.
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http://dx.doi.org/10.1021/acs.nanolett.9b04095DOI Listing
January 2020

CD4 T Responses Other Than Th1 Type Are Preferentially Induced by Latency-Associated Antigens in the State of Latent Infection.

Front Immunol 2019 29;10:2807. Epub 2019 Nov 29.

Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.

() produces a diverse range of antigenic proteins in its dormant phase. The cytokine profiles of CD4 T cell responses, especially subsets other than Th1 type (non-Th1 type), against these latency-associated antigens such as α-crystallin (Acr), heparin-binding hemagglutinin (HBHA), and mycobacterial DNA-binding protein 1 (MDP-1) remain elusive in relation to the clinical stage of infection. In the present study, peripheral blood mononuclear cells (PBMCs) collected from different stages of -infected cases and control PBMCs were stimulated with these antigens and ESAT-6/CFP-10. Cytokine profiles of CD4 T cells were evaluated by intracellular cytokine staining using multicolor flow cytometry. Our results demonstrate that Th1 cytokine responses were predominant after TB onset independent of the type of antigen stimulation. On the contrary, non-Th1 cytokine responses were preferentially induced by latency-associated antigens, specifically IL-10 response against Acr in latent infection. From these results, we surmise a shift in the CD4 T cell response from mixed non-Th1 to Th1 dominant type during TB progression.
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http://dx.doi.org/10.3389/fimmu.2019.02807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6897369PMC
November 2020

Electrochemical determination of uric acid in urine and serum with uricase/carbon nanotube /carboxymethylcellulose electrode.

Anal Biochem 2020 02 10;590:113533. Epub 2019 Dec 10.

Tsuruga Institute of Biotechnology, Toyobo Co., Ltd, 10-24 Toyo-cho, Tsuruga, Fukui, 914-8550, Japan.

The detection of uric acid in blood and urine is clinically important in terms of suitable diagnosis and self-healthcare. An amperometric thin film biosensor composed of carbon nanotube and uricase enzyme is presented. The CNT is successfully dispersed in aqueous solution with carboxymethylcellulose surfactant. This enables thin film formation by a simple drop-casting layer-by-layer process. The uricase/carboxymethylcellulose dispersed carbon nanotube/gold thin film biosensor shows the best sensing performance compared to that with sodium cholate surfactant in terms of higher current and lower detection potential. The presented procedure shows good performance with neither electron transfer mediator nor complicated process. Cyclic voltammetry exhibited a sensitivity of 233 μA mM cm at +0.35 V, a linear range of 0.02-2.7 mM, and a detection limit of 2.8 μM. We quantify and graph uric acid data in actual physiological samples (serum and urine) for the first time and detection values showed good agreement with those obtained by a conventional analytical method (enzymatic colorimetry kit).
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http://dx.doi.org/10.1016/j.ab.2019.113533DOI Listing
February 2020

Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants.

Vet Res 2019 Nov 14;50(1):94. Epub 2019 Nov 14.

Division of Bacterial and Parasitic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Japan.

Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections.
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http://dx.doi.org/10.1186/s13567-019-0708-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854688PMC
November 2019
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