Publications by authors named "Takahiro Yamamoto"

135 Publications

Cooperative methylation of human tRNA3Lys at positions A58 and U54 drives the early and late steps of HIV-1 replication.

Nucleic Acids Res 2021 Oct 13. Epub 2021 Oct 13.

Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Kumamoto 860-8556, Japan.

Retroviral infection requires reverse transcription, and the reverse transcriptase (RT) uses cellular tRNA as its primer. In humans, the TRMT6-TRMT61A methyltransferase complex incorporates N1-methyladenosine modification at tRNA position 58 (m1A58); however, the role of m1A58 as an RT-stop site during retroviral infection has remained questionable. Here, we constructed TRMT6 mutant cells to determine the roles of m1A in HIV-1 infection. We confirmed that tRNA3Lys m1A58 was required for in vitro plus-strand strong-stop by RT. Accordingly, infectivity of VSV-G pseudotyped HIV-1 decreased when the virus contained m1A58-deficient tRNA3Lys instead of m1A58-modified tRNA3Lys. In TRMT6 mutant cells, the global protein synthesis rate was equivalent to that of wild-type cells. However, unexpectedly, plasmid-derived HIV-1 expression showed that TRMT6 mutant cells decreased accumulation of HIV-1 capsid, integrase, Tat, Gag, and GagPol proteins without reduction of HIV-1 RNAs in cells, and fewer viruses were produced. Moreover, the importance of 5,2'-O-dimethyluridine at U54 of tRNA3Lys as a second RT-stop site was supported by conservation of retroviral genome-tRNALys sequence-complementarity, and TRMT6 was required for efficient 5-methylation of U54. These findings illuminate the fundamental importance of tRNA m1A58 modification in both the early and late steps of HIV-1 replication, as well as in the cellular tRNA modification network.
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http://dx.doi.org/10.1093/nar/gkab879DOI Listing
October 2021

Sequestration of RBM10 in Nuclear Bodies: Targeting Sequences and Biological Significance.

Int J Mol Sci 2021 Sep 29;22(19). Epub 2021 Sep 29.

Department of Immunology, Graduate School of Medicine, Osaka City University, Osaka 545-8585, Japan.

RBM10 is an RNA-binding protein that regulates alternative splicing (AS). It localizes to the extra-nucleolar nucleoplasm and S1-1 nuclear bodies (NBs) in the nucleus. We investigated the biological significance of this localization in relation to its molecular function. Our analyses, employing deletion mutants, revealed that RBM10 possesses two S1-1 NB-targeting sequences (NBTSs), one in the KEKE motif region and another in the CH Zn finger (ZnF). These NBTSs act synergistically to localize RBM10 to S1-1 NBs. The CH ZnF not only acts as an NBTS, but is also essential for AS regulation by RBM10. Moreover, RBM10 does not participate in S1-1 NB formation, and without alterations of RBM10 protein levels, its NB-localization changes, increasing as cellular transcriptional activity declines, and vice versa. These results indicate that RBM10 is a transient component of S1-1 NBs and is sequestered in NBs via its NBTSs when cellular transcription decreases. We propose that the CH ZnF exerts its NB-targeting activity when RBM10 is unbound by pre-mRNAs, and that NB-localization of RBM10 is a mechanism to control its AS activity in the nucleus.
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http://dx.doi.org/10.3390/ijms221910526DOI Listing
September 2021

Characterization of the Structural Diversity and Structure-Specific Behavior of Oxidized Phospholipids by LC-MS/MS.

Chem Pharm Bull (Tokyo) 2021 ;69(10):953-961

Division of Physiological Chemistry and Metabolism, Keio University Faculty of Pharmacy.

Polyunsaturated fatty acids (PUFAs), esterified to phospholipids, are susceptible to oxidation. They form oxidized phospholipids (OxPLs) by oxygenases or reactive oxygen species (ROS), or both. These OxPLs are associated with various diseases, such as atherosclerosis, pulmonary injuries, neurodegenerative diseases, cancer, and diabetes. Since many types of OxPLs seem to be generated in vivo, precise determination of their structural diversity is required to understand their potential structure-specific functions. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful method to quantitatively measure the structural diversity of OxPLs present in biological samples. This review outlines recent advances in analytical methods for OxPLs and their physiological relevance in health and diseases.
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http://dx.doi.org/10.1248/cpb.c21-00274DOI Listing
January 2021

[Surgical Resection Papillary Fibroelastoma Arising from Left Atrium:Report of a Case].

Kyobu Geka 2021 Oct;74(11):967-971

Department of Cardiovascular Surgery, Jichi Medical University Saitama Medical Center, Saitama, Japan.

A 75-year-old man was admitted for cerebral infarction. Magnetic resonance imaging revealed parietal lobe cerebral infarction. Transesophageal echo and contrast-enhanced computed tomography indicated mobile and speckled mass arising from left atrium. He was diagnosed with cardiogenic cerebral embolism. Under cardiopulmonary bypass, resection of the mass including endocardium tissue was per formed. The resected specimen showed multiple small fronds resembling a sea anemone. Microscopic examination showed multiple branching fronds of paucicellular and avascular fibroelastic tissue lined by a single layer of endocardium. Pathological diagnosis was papillary fibroelastoma. Three years passed without recurrence.
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October 2021

Macrophage/microglia-derived IL-1β induces glioblastoma growth via the STAT3/NF-κB pathway.

Hum Cell 2021 Sep 30. Epub 2021 Sep 30.

Department of Neurosurgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.

Glioblastoma is a glioma characterized by highly malignant features. Numerous studies conducted on the relationship between glioblastoma and the microenvironment have indicated the significance of tumor-associated macrophages/microglia (TAMs) in glioblastoma progression. Since interleukin (IL)-1β secreted by TAMs has been suggested to promote glioblastoma growth, we attempted to elucidate the detailed mechanisms of IL-1β in glioblastoma growth in this study. A phospho-receptor tyrosine kinase array and RNA-sequencing studies indicated that IL-1β induced the activation of signal transducer and activator of transcription-3 and nuclear factor-kappa B signaling. Glioblastoma cells stimulated by IL-1β induced the production of IL-6 and CXCL8, which synergistically promoted glioblastoma growth via signal transducer and activator of transcription-3 and nuclear factor-kappa B signaling. By immunohistochemistry, IL-1β expression was seen on TAMs, especially in perinecrotic areas. These results suggest that IL-1β might be a useful target molecule for anti-glioblastoma therapy.
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http://dx.doi.org/10.1007/s13577-021-00619-8DOI Listing
September 2021

Chirality Distributions for Semiconducting Single-Walled Carbon Nanotubes Determined by Photoluminescence Spectroscopy.

Nanomaterials (Basel) 2021 Sep 6;11(9). Epub 2021 Sep 6.

Department of Physics, Tokyo University of Science, Shinjuku, Tokyo 162-8601, Japan.

To realize single-walled carbon nanotube (SWCNT) chiral selective growth, elucidating the mechanism of SWCNT chirality (n,m) selectivity is important. For this purpose, an accurate evaluation method for evaluating the chirality distribution of grown SWCNTs without post-growth processing or liquid-dispersion of SWCNTs is indispensable. Here, we used photoluminescence spectroscopy to directly measure the chirality distributions of individual semiconducting SWCNTs suspended on a pillar-patterned substrate. The number of chirality-assigned SWCNTs was up to 332 and 17 chirality types with the chiral angles ranging from 0° to 28.05° were detected. The growth yield of SWCNTs was confirmed to primarily depends on the chiral angle in accordance with the screw dislocation model. Furthermore, when higher-yield chiralities are selected, the chiral angle distribution with a peak corresponding to near-armchair SWCNTs is well fitted with a model that incorporates the thermodynamic effect at the SWCNT-catalyst interface into the kink growth-based kinetic model. Our quantitative and statistical data provide new insights into SWCNT growth mechanism as well as experimental confirmation of theoretical predictions.
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http://dx.doi.org/10.3390/nano11092309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465080PMC
September 2021

Photohardenable Pressure-Sensitive Adhesives using Poly(methyl methacrylate) containing Liquid Crystal Plasticizers.

ACS Appl Mater Interfaces 2021 Aug 12;13(33):39949-39956. Epub 2021 Aug 12.

Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.

Hardenable pressure-sensitive adhesives, which show pressure-sensitive adhesion state with weak adhesion strength in their initial semisolid state and general adhesion state with strong adhesion strength in their hardened state, are desirable in various industrial fields to improve efficiency of manufacturing and recycling products. Here we developed novel photohardenable pressure-sensitive adhesives triggered by photoplasticization of poly(methyl methacrylate) containing photoresponsive liquid crystal (nematic and smectic E) plasticizers at various ratios. It was found that photoplasticization, which is the photoinduced reduction of glass transition temperature and hardness of polymers, could be repeatedly induced by alternate irradiation with ultraviolet (UV) and visible (Vis) light in all mixtures, regardless of the phase structures of the photoresponsive plasticizers. Upon photoplasticization under UV-light irradiation, all mixtures exhibited glassy-to-rubbery transition to a pressure-sensitive adhesion state under appropriate conditions. Upon irradiation of the photoplasticized samples with Vis light, the samples recovered their initial hardened state, recovering the glassy nature with elastic moduli. The adhesion strength of the samples in the hardened state was significantly influenced by the phase structures of the plasticizers. When a photoresponsive plasticizer exhibited the smectic E phase, which is a highly ordered liquid-crystalline phase, the adhesion strength was remarkably larger than those of the case using the plasticizers showing nematic and crystalline phases. This result was reasonably explained in terms of the suppressed bleed-out of the photoresponsive plasticizers from the polymer and the good mechanical properties of the mixture stemming from the characteristics of the smectic E phase. Furthermore, through the reversibility of a photoplasticization process, we achieved a photoinduced reduction of the adhesion strength by UV irradiation of the samples in the hardened state. Photohardenable pressure-sensitive adhesives with reversibility has been developed using a commodity plastic just by adding the photoresponsive plasticizer showing the smectic E phase.
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http://dx.doi.org/10.1021/acsami.1c11634DOI Listing
August 2021

[Rapid Growing Thoracic Aortic Aneurysm in a Patient with Relapsing Polychondritis].

Kyobu Geka 2021 Aug;74(8):583-586

Department of Cardiovascular Surgery, Jichi Medical University Saitama Medical Center, Saitama, Japan.

A 26-year-old man with relapsing polychondritis was admitted for the treatment of multiple thoracic aortic aneurysms in the ascending and descending aorta. Descending thoracic aortic aneurysm showed rapid expansion, therefore, the patient underwent an extended thoracic aortic repair from the ascending aorta to the descending aorta via anterolateral thoracotomy and partial sternotomy. Although postoperative course was uneventful, aortic root enlargement and severe aortic insufficiency progressed over the next two years. He and his family refused redo surgical intervention and the patient died of heart failure. Careful perioperative follow-up may be mandatory in a patient with relapsing polychondritis complicated by cardiovascular disease.
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August 2021

In vitro functional analysis of four variants of human asparagine synthetase.

J Inherit Metab Dis 2021 09 9;44(5):1226-1234. Epub 2021 Jun 9.

Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan.

The loss-of-function variants of the human asparagine synthetase (ASNS) gene cause asparagine synthetase deficiency (ASNSD). Diagnosis of ASNSD requires genetic tests because a specific biochemical diagnostic for ASNSD is not available. There are a few reports describing the functional evaluation of ASNS variants. Therefore, in vitro methods are needed to evaluate the detected variants in patients. In this report, five types of human ASNS proteins (wild-type and our reported four variants: p.Leu145Ser, p.Leu247Trp, p.Val489Asp, and p.Trp541Cysfs*5) were expressed in silkworm using a baculoviral expression system. An enzymatic activity assay of ASNS was performed, and the concentration of asparagine by ninhydrin and High Performance Liquid Chromatography methods using the purified recombinant proteins was measured. We established ASNS deficient HEK293 cells using the CRISPR/Cas9 method and evaluated the growth of cells without asparagine after transduction of ASNS variants with a lentiviral expression system. The four ASNS variants displayed significantly low enzymatic activity. The ASNS deficient HEK293 cells transduced with wild-type ASNS grew without asparagine, whereas cells transduced with the variants did not grow or showed significantly slower growth than cells transduced with wild-type ASNS. Herein, we established a method for evaluating the enzymatic activity of the recombinant human ASNS variants. The results of the cell-based assay corroborated the results of the enzymatic activity. These methods should enable the evaluation of the pathogenicity of ASNS variants.
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http://dx.doi.org/10.1002/jimd.12408DOI Listing
September 2021

Importance of [Formula: see text] orbital and electron correlation in iron-based superconductors revealed by phase diagram for 1111-system.

Sci Rep 2021 May 11;11(1):10006. Epub 2021 May 11.

Department of Physics, Osaka University, Toyonaka, Osaka, 560-0043, Japan.

The structural flexibility at three substitution sites in LaFeAsO enabled investigation of the relation between superconductivity and structural parameters over a wide range of crystal compositions. Substitutions of Nd for La, Sb or P for As, and F or H for O were performed. All these substitutions modify the local structural parameters, while the F/H-substitution also changes band filling. It was found that the superconducting transition temperature [Formula: see text] is strongly affected by the pnictogen height [Formula: see text] from the Fe-plane that controls the electron correlation strength and the size of the [Formula: see text] hole Fermi surface (FS). With increasing [Formula: see text], weak coupling BCS superconductivity switches to the strong coupling non-BCS one where electron correlations and the [Formula: see text] hole FS may be important.
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http://dx.doi.org/10.1038/s41598-021-89231-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113352PMC
May 2021

Learning effectiveness of using augmented reality technology in central venous access procedure: an experiment using phantom and head-mounted display.

Int J Comput Assist Radiol Surg 2021 Jun 16;16(6):1069-1074. Epub 2021 Apr 16.

Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women's Medical University, 8-1, Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.

Purpose: Augmented reality (AR) technology improves the learning process in interventional radiology. This study hypothesized that using AR to train for central venous access is superior to using ultrasound alone.

Methods: This study used an AR central venous catheterization phantom with an internal jugular vein (IJV) and subclavian vein (SCV) made of resin body and soft tubing. Ten radiologists attempted to punctuate, using needle placement simulation, under three conditions (ultrasound-, augmented reality-, and ultrasound and AR-guided methods; US-only, AR-only, and US+AR, respectively) using a smart-glass device (HoloLens, Microsoft, Redmond, WA, USA). Subjective (anatomical understanding and self-confidence for procedure) and objective evaluations (optimized needle position and time) were recorded for each condition.

Results: The subjective IJV evaluation showed no difference among the guiding methods (p = 0.26 and p = 0.07 for anatomical understanding and self-confidence for procedure, respectively). Conversely, there were significant improvements in subjective and objective evaluations for SCV using the AR-only and US+AR methods (p < 0.05) and US+AR method (p < 0.05), respectively. The AR-only method reduced the time required to fix the needle position to puncture the SCV (p < 0.05), but its objective evaluation did not improve compared with the US-only method (p = 0.20).

Conclusion: Adding the AR-guided method to the US-guided method improved subjective and objective evaluations in the SVC procedure. The AR technology-assisted training may be more beneficial for use in difficult procedures. Though the AR-only method saved time, no time saving is expected with AR+US method.
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http://dx.doi.org/10.1007/s11548-021-02365-6DOI Listing
June 2021

Granulomatosis with polyangiitis presenting as a solitary renal mass: A case report with imaging and literature review.

Radiol Case Rep 2021 Mar 17;16(3):736-741. Epub 2021 Jan 17.

Department of Radiology, Aichi Medical University, 1-1 Yazako Karimata, Yatomi City, Aichi 480-1195, Japan.

Granulomatosis with polyangiitis (GPA) manifests as necrotizing granulomatous inflammatory masses in the nasal cavity, paranasal sinuses, and lungs. However, a mass in the kidney is extremely rare. We herein report a case of GPA that presented as a solitary mass in the left kidney. The patient was a man in his 60s. A 2.5-cm solitary mass was incidentally discovered in the left kidney at another hospital and was followed-up. Eight months later, the mass had enlarged, and the patient visited our hospital for further examination and treatment. The mass was hypovascular, with unclear margins on contrast-enhanced computed tomography (CT). The signal of the mass was nonuniform and iso- to slightly hypo-intense on T2-weighted and diffusion-weighted magnetic resonance imaging (MRI). Enlarged para-aortic lymph nodes were also detected on the CT and MRI. Based on imaging, malignant tumors were suspected, and nephrectomy was performed. The pathological diagnosis was GPA. We performed a literature review of this rare renal manifestation and present a summary of reported imaging findings. If a hypovascular renal mass with an unclear margin can be found in those with GPA, unnecessary operations may be avoided by actively promoting renal biopsy.
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http://dx.doi.org/10.1016/j.radcr.2021.01.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815982PMC
March 2021

New treatment strategy for endometriosis using progestin-primed ovarian stimulation with dienogest: A prospective cohort study, comparison of dienogest versus dydrogesterone.

Reprod Biol 2021 Mar 14;21(1):100470. Epub 2020 Dec 14.

Kamiya Ladies Clinic, 2-1, Nishi 2, Kita 3, Chuo-ku, Sapporo, Hokkaido, 060-0003, Japan.

Dienogest (DNG) is an oral progestin effective for the treatment of symptomatic endometriosis, such as reduction of endometrial lesion and control of pain intensity. Progestin-primed ovarian stimulation (PPOS) is a new controlled ovarian hyperstimulation (COH) regimen, and several reports have shown that dydrogesterone (DYG) is an appropriate progestin for PPOS. The purpose of this study was to evaluate the efficacy of DNG in patients undergoing PPOS during COH in comparison with DYG. This was a prospective, cohort, parallel-group, non-inferiority trial of 150 women with endometriosis undergoing assisted reproductive technology between February 2018 and May 2020 at the single fertility center. The assignment to each protocol was based on the optimal treatment for each patient. Patients taking DNG 2 mg continuously were assigned in the DNG group(n = 73). The other patients were allocated in DYG group(n = 77). All viable embryos were cryopreserved for subsequent transfer. The main outcome measures were the mature oocyte and fertilization rates. During this study, no premature LH surge was detected. A smaller number of oocytes were retrieved in the DNG group than in the DYG group (6.18 ± 3.60 vs. 9.85 ± 5.77); however, the rate of mature oocytes was significantly higher in the DNG group than in the DYG group (89.1 % vs. 78.9 %). The fertilization rate was comparable between two groups. Therefore, patients taking DNG for PPOS can continue endometriosis treatment and obtain good-quality embryos during COH. Further prospective randomized-controlled trial should be performed to confirm of this novel strategy of DNG.
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http://dx.doi.org/10.1016/j.repbio.2020.100470DOI Listing
March 2021

Light Response of KT2440 Mediated by Class II LitR, a Photosensor Homolog.

J Bacteriol 2020 09 23;202(20). Epub 2020 Sep 23.

Life Science Research Center, College of Bioresource Sciences, Nihon University, Tokyo, Japan

KT2440 retains three homologs (PplR1 to PplR3) of the LitR/CarH family, an adenosyl B-dependent light-sensitive MerR family transcriptional regulator. Transcriptome analysis revealed the existence of a number of photoinducible genes, including , (encoding DNA photolyase), (furan-containing fatty acid synthase), (GTP cyclohydrolase I), (cryptochrome-like protein), and multiple genes without annotated/known function. Transcriptional analysis by quantitative reverse transcription-PCR with knockout mutants of to showed that a triple knockout completely abolished the light-inducible transcription in , which indicates the occurrence of ternary regulation of PplR proteins. A DNase I footprint assay showed that PplR1 protein specifically binds to the promoter regions of light-inducible genes, suggesting a consensus PplR1-binding direct repeat, 5'-T(G/A)TACANTGTA(C/T)A-3'. The disruption of B biosynthesis cluster did not affect the light-inducible transcription; however, disruption of (where LOV indicates "light, oxygen, or voltage") and , encoding blue light photoreceptors adjacently located to and , respectively, led to the complete loss of light-inducible transcription. Overall, the results suggest that the three PplRs and two PpSB-LOVs cooperatively regulate the light-inducible gene expression. The wide distribution of the / cognate pair homologs in spp. and related bacteria suggests that the response and adaptation to light are similarly regulated in the group of nonphototrophic bacteria. The LitR/CarH family is a new group of photosensor homologous to MerR-type transcriptional regulators. Proteins of this family are distributed to various nonphototrophic bacteria and grouped into at least five classes (I to V). retaining three class II LitR proteins exhibited a genome-wide response to light. All three paralogs were functional and mediated photodependent activation of promoters directing the transcription of light-induced genes or operons. Two LOV (light, oxygen, or voltage) domain proteins, adjacently encoded by two genes, were also essential for the photodependent transcriptional control. Despite the difference in light-sensing mechanisms, the DNA binding consensus of class II LitR [T(G/A)TA(C/T)A] was the same as that of class I. This is the first study showing the actual involvement of class II LitR in light-induced transcription.
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http://dx.doi.org/10.1128/JB.00146-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515241PMC
September 2020

Mid-term outcome of transarterial embolization of renal artery pseudoaneurysm and arteriovenous fistula after partial nephrectomy screened by early postoperative contrast-enhanced CT.

CVIR Endovasc 2020 Sep 16;3(1):68. Epub 2020 Sep 16.

Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women's Medical University, 8-1, Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.

Purpose: To retrospectively evaluate the mid-term outcome of transarterial embolization (TAE) of renal artery pseudoaneurysm (RAP) including arteriovenous fistula (AVF) after partial nephrectomy screened by early postoperative contrast-enhanced CT (CE-CT).

Materials And Methods: Eighty-two patients (7.0%) who underwent TAE after partial nephrectomy were reviewed, from 1166 partial nephrectomies performed over 6 years. In 18 patients (22.0%), TAE was performed emergently on the median postoperative day (POD) seven. In the remaining patients, elective TAE was performed on the median POD six for RAP detected by early postoperative CE-CT or that emerged on follow-up CE-CT.

Results: In one patient (1.2%), TAE was performed twice because one of two RAPs could not be embolized during the first TAE, being successfully embolized at the second TAE after readmission with hematuria. Otherwise, no bleeding recurrence or RAPs were observed during the median 1354 follow-up days. Thus, the primary and secondary success rates of TAE were 98.8% (81 of 82 patients) and 100% (82 of 82 patients), respectively. On angiography, the average number of lesions was 1.7 ± 0.9 and the average RAP size was 12.8 ± 6.0 mm. The shapes of the lesions varied: oval-round 60, oval-round + AVF 36, irregular + AVF 14, AVF 12, irregular 10, disruption 4, and extravasation 3. No major complications were observed. The median inpatient days after TAE were two. No estimated glomerular filtration rate deterioration was observed (64.6 ± 18.6 vs. 64.2 ± 18.4 mL/min/1.73 m, p = 0.902).

Conclusion: TAE is largely effective and safe for treating bleedings or RAPs, including AVFs, after partial nephrectomy, as screened by early postoperative CE-CT.
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http://dx.doi.org/10.1186/s42155-020-00160-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494709PMC
September 2020

Stent graft treatment for coronary sinus hemorrhage associated with esophageal cancer.

Minim Invasive Ther Allied Technol 2020 Jun 5:1-4. Epub 2020 Jun 5.

Department of Radiology, Aichi Medical University, Aichi, Japan.

Hemorrhage arising from the coronary sinus is very rare and can be lethal. It has historically been treated surgically. The present patient had coronary sinus rupture secondary to esophageal cancer and an abscess in the pericardium. Due to her poor general status, this patient was contraindicated for surgery and underwent endovascular therapy. The hemorrhage was treated by stent graft deployment and the patient was temporarily discharged. Two months later, CT showed that the stent graft was occluded by thrombosis. The patient died without hemorrhage 2.5 months thereafter.
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http://dx.doi.org/10.1080/13645706.2020.1771372DOI Listing
June 2020

One-stage free transfer of latissimus dorsi-serratus anterior combined muscle flap with dual innervation for smile reanimation in established facial paralysis.

J Plast Reconstr Aesthet Surg 2020 Jun 31;73(6):1107-1115. Epub 2020 Jan 31.

Department of Plastic, Aesthetic, and Reconstructive Surgery, Tokyo Metropolitan Police Hospital, Tokyo, Japan.

Microneurovascular free muscle transfer is the gold-standard surgical procedure for the reanimation of established facial paralysis. However, the innervation of the transferred muscle by the contralateral facial nerve is usually insufficient to produce a stable smile. Besides, the corner of the mouth sometimes moves unnaturally as if it were being pulled up because of the single-direction movement. Thus, we propose one-stage facial reanimation using free latissimus dorsi (LD)-serratus anterior (SA) combined muscle flap transfer with dual innervation. The LD-SA combined muscle flap was harvested with the thoracodorsal artery and vein as common vessels to move the corner of the mouth bidirectionally for natural smiling. The LD muscle was located in the same direction as the zygomaticus major muscle and reinnervated by dual innervation. The contralateral facial nerve was coapted with the thoracodorsal nerve, and the ipsilateral masseter motor nerve was inserted into the LD muscle by intramuscular neurotization. The 6th or 7th SA muscle was located in the same direction as the risorius muscle and reinnervated by neurorrhaphy of the long thoracic nerve and the thin branch of the ipsilateral masseter motor nerve. Since 2015, seven patients have been treated with this method without complications. On average, SA muscle movement was detected in voluntary biting at 3.1 months, and spontaneous smiling occurred 7.7 months after surgery. All patients developed a spontaneous natural smile. This method for established facial paralysis has the potential to improve the quality of the reconstructed smile and the unstable results of conventional single-innervation-single-muscle transfer.
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http://dx.doi.org/10.1016/j.bjps.2020.01.032DOI Listing
June 2020

Dehydroeffusol Rescues Amyloid β-Induced Spatial Working Memory Deficit.

Plant Foods Hum Nutr 2020 Jun;75(2):279-282

Department of Neurophysiology, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.

Amyloid β (Aβ) peptides produced from the amyloid precursor protein, a transmembrane protein, are neurotoxic and blocking the neurotoxicity may lead to prevention of Alzheimer's disease (AD). Here we tested whether Aβ-induced cognitive decline is rescued by treatment with dehydroeffusol, a phenanthrene isolated from Chinese medicine Juncus effusus. Dehydroeffusol (5 ~ 15 mg/kg body weight) was orally administered to mice for 6 days and Aβ (2 mM) was injected at the rate of 1 μl/min for 3 min into the lateral ventricle. Y-maze test was performed after dehydroeffusol administration for 12 days. Aβ impaired learning and memory in the test, while the impairment was dose-dependently rescued by dehydroeffusol administration. The present study indicates that treatment with dehydroeffusol is effective for rescuing Aβ-induced cognitive decline.
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http://dx.doi.org/10.1007/s11130-020-00816-0DOI Listing
June 2020

Safety and feasibility of unilateral double femoral venous access including double sheath insertion via a single-hole method for adrenal venous sampling.

Jpn J Radiol 2020 Aug 13;38(8):800-806. Epub 2020 Apr 13.

Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women's Medical University Hospital, 8-1, Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.

Purpose: To assess the safety and feasibility of unilateral double femoral venous access including double sheath insertion via a single-hole method (two-in-one method) for adrenal venous sampling (AVS).

Materials And Methods: Two 5-Fr sheaths were percutaneously inserted into a single femoral vein for AVS in 324 patients. Two needles were inserted sequentially in tandem under ultrasound guidance; furthermore, two sheaths were individually inserted (two-in-two method) if both punctures were successfully achieved. In the presence of a single puncture, two sheaths were inserted through two guidewires and a temporarily inserted sheath (two-in-one method). This protocol was repeated until two sheaths were successfully inserted. Thus, two sheaths were inserted with the two-in-one method in 56 patients (17.3%) and two-in-two method in 268 patients (82.7%).

Results: No significant differences in the technical success rates of AVS were observed (p = 0.067-0.647). Minor groin hematoma was observed more frequently in the two-in-one than in the two-in-two method (8.9% vs. 2.6%, p = 0.039). No major groin hematoma, deep venous thrombosis, or pulmonary embolization was observed. The mean number of needle puncture was 2.5 ± 1.3 and duration of inserting two sheaths 5.1 ± 3.9 min.

Conclusion: Unilateral double femoral venous access including the two-in-one method is safe and feasible for AVS.
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http://dx.doi.org/10.1007/s11604-020-00965-xDOI Listing
August 2020

A case of childhood glaucoma with a combined partial monosomy 6p25 and partial trisomy 18p11 due to an unbalanced translocation.

Ophthalmic Genet 2020 04 30;41(2):175-182. Epub 2020 Mar 30.

Department of Ophthalmology, Hamamatsu University School of Medicine, Hamamatsu, Japan.

: Chromosomal deletion involving the 6p25 region results in a clinically recognizable syndrome characterized by anterior eye chamber anomalies with risk of glaucoma and non-ocular malformations (6p25 deletion syndrome). We report a newborn infant case of childhood glaucoma with a combination of partial monosomy 6p25 and partial trisomy 18p11 due to an unbalanced translocation.: The patient was a 0-year-old girl. Both eyes showed aniridia and left eye Peters anomaly with multiple malformations. To identify the chromosomal aberrations in the patient with clinically suspected 6p25 deletion syndrome, we performed cytogenetic analysis (G-banding and multicolor fluorescent hybridization) and array-based comparative genomic hybridization (array-CGH) analysis.: Cytogenetic analyses revealed a derivative chromosome 6 with its distal short arm replaced by an extra copy of the short arm of chromosome 18. Array-CGH analysis detected a 4.6-Mb deletion at 6pter to 6p25.1 and 8.9-Mb duplication at 18pter to 18p11.22. To determine the breakpoint of the unbalanced rearrangement at the single-base level, we performed a long-range PCR for amplifying the junctional fragment of the translocation breakpoint. By sequencing the junctional fragment, we defined the unbalanced translocation as g.chr6:pter_4594783delinschr18:pter_8911541.: A phenotype corresponding to combined monosomy 6p25 and trisomy 18p11 presented as childhood glaucoma associated with non-acquired (congenital) ocular anomalies consist of aniridia and Peters anomaly and other systemic malformations. To the best of our knowledge, this is the first report which demonstrated the breakpoint sequence of an unbalanced translocation in a Japanese infant with childhood glaucoma.
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http://dx.doi.org/10.1080/13816810.2020.1744019DOI Listing
April 2020

Novel variants of ABCC9 in Japanese children with Cantú syndrome.

Pediatr Int 2020 Mar 21;62(3):410-412. Epub 2020 Mar 21.

Department of Pediatrics, Gifu University Graduate School of Medicine, Gifu, Japan.

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http://dx.doi.org/10.1111/ped.14098DOI Listing
March 2020

Endometrial cancer arising from adenomyosis: Case report and literature review of MRI findings.

Radiol Case Rep 2020 Apr 13;15(4):427-430. Epub 2020 Feb 13.

Department of Radiology, Aichi Medical University, 1-1 YazakoKarimata, Nagakute 480-1195, Japan.

Endometrial cancer arising from adenomyosis (EC-AIA) is extremely rare, and the typical magnetic resonance imaging (MRI) findings of EC-AIA have not been established. We report a case of EC-AIA that was detected preoperatively on MRI and conduct a literature review of the MRI findings of EC-AIA.
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http://dx.doi.org/10.1016/j.radcr.2020.01.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031131PMC
April 2020

Evaluating the potential area and environmental key factors for Sargassum bed restoration in highly turbid waters of the northwestern Arabian Gulf.

Mar Pollut Bull 2020 Apr 12;153:110949. Epub 2020 Feb 12.

Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Salmiya 22017, Kuwait.

This study evaluates the potential area and the key environmental factors supporting Sargassum bed restoration (SBR) in the highly turbid northwestern Arabian Gulf where rapid coastal development impinges on the marine ecosystem functioning. Water depth was a primary environmental factor governing the distribution of the subtidal macroalgae beds in these turbid waters. The relationship between Sargassum coverage and water depth measured by an echo sounder indicated optimal water depths where the maximum coverage was observed. The availability of stable hard substrate was another key factor introducing heterogeneity in Sargassum coverage. Potential area for the SBR estimated based on the optimal depths was 4.26 km, whereas only 50% of the potential area (2.19 km) was currently vegetated due to the absence of hard substrate. The outcomes of this study offer beneficial information toward implementation of the SBR as a part of mitigation measures in future coastal development plans in the region.
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http://dx.doi.org/10.1016/j.marpolbul.2020.110949DOI Listing
April 2020

Image quality of early postoperative CT angiography with reduced contrast material and radiation dose using model-based iterative reconstruction for screening of renal pseudoaneurysms after partial nephrectomy.

Eur J Radiol 2020 Mar 28;124:108853. Epub 2020 Jan 28.

Departments of Urology, Tokyo Women's Medical University Hospital, Tokyo, Japan.

Purpose: To evaluate the image quality of early postoperative CT angiography with low contrast material and radiation dose using model-based iterative reconstruction (FIRST) for screening pseudoaneurysms after partial nephrectomy.

Methods: CT angiography was obtained before surgery using conventional iterative dose reduction reconstruction (AIDR 3D) with 120 kVp and 600 mgI/kg of contrast material and obtained after partial nephrectomy using FIRST with 80-100 kVp and 360 mgI/kg in 35 patients. Contrast-to-noise ratio, visual image quality scores using a 5-point scale, and longest length of the unaffected renal arteries on maximum intensity projection images were retrospectively compared between FIRST and AIDR 3D.

Results: No significant differences existed in contrast-to-noise ratio or image quality scores of the renal arteries between FIRST and AIDR 3D (25.8 ± 6.6 vs. 25.4 ± 7.0, p = 0.991 and 4.8 ± 0.4 vs. 4.5 ± 0.9, p = 0.515, respectively). Visualization scores and longest length of the peripheral renal arteries in FIRST were significantly superior to those of AIDR 3D (4.3 ± 0.8 vs. 3.5 ± 1.0, p < 0.001 and 100.4 ± 14.9 mm vs. 90.2 ± 15.7 mm, p = 0.010, respectively). The dose-length product with FIRST was significantly lower than that with AIDR 3D (566.1 ± 217.4 mGy.cm vs. 829.8 ± 324.9 mGy.cm, p < 0.001).

Conclusion: FIRST can improve visualization of the peripheral renal arteries with contrast material and radiation dose reduced by approximately 30 % compared with AIDR 3D, which enables adequate evaluation of pseudoaneurysms after partial nephrectomy.
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http://dx.doi.org/10.1016/j.ejrad.2020.108853DOI Listing
March 2020

Beneficial Effect of Carotid Artery Stenting on Cerebral Hemodynamic Impairment and Cognitive Function.

Neurol Med Chir (Tokyo) 2020 Feb 21;60(2):66-74. Epub 2019 Nov 21.

Department of Neurosurgery, Toshima Hospital, Tokyo Metropolitan Health and Hospitals Corporation.

The aim of this study was to evaluate the effects of the carotid artery stenting (CAS) procedure on cerebral hemodynamics and cognitive performance in patients with internal carotid artery stenosis. In this prospective observational study, 16 consecutive patients in a single institute were treated with CAS from 2010 to 2011 (71.7 ± 7.3 years old, 12 males, and six left-sided procedures). Before and after the CAS procedure, all patients were evaluated with single photon emission computed tomography using 123I-iodoamphetamine and acetazolamide as well as the Weschler Adult Intelligent Scale (WAIS) to assess three intelligent quotients and four indexes. Patients with decreased preprocedural cerebral blood flow (CBF) and cerebrovascular reserve (CVR) experienced significant increases in CBF and CVR (P = 0.01 and 0.03). Twelve (75%) patients experienced a significant increase in one or more WAIS scores, while two (13%) showed a significant decrease. The most frequently improved scores were the Working Memory Index and Processing Speed Index (seven patients/44%). Preprocedural CBF was significantly correlated with the Full-scale and Performance Intelligent Quotients (r = 0.51-0.56, P = 0.02-0.04), and patients who experienced a significant increase in these scores had larger increases in CVR than patients with unchanged scores (P = 0.0097-0.019). These results indicate that the CAS procedure improved impaired cerebral hemodynamics and might benefit the cognitive function of patients with internal carotid artery stenosis related to impaired cerebral hemodynamics.
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http://dx.doi.org/10.2176/nmc.oa.2019-0147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040432PMC
February 2020

Humidity control effect of vapor-permeable walls employing hygroscopic insulation material.

Indoor Air 2020 03 10;30(2):346-360. Epub 2019 Dec 10.

Faculty of Human-Environment Studies, Kyushu University, Fukuoka, Japan.

Airtight construction and high-performance thermal insulation materials are commonly considered important building features to enhance indoor thermal comfort while reducing thermal load. However, when water vapor is generated in such airtight indoor spaces, it cannot be discharged to the outside, causing interstitial condensation and subsequent intrusion of moisture into the walls. Hygroscopic building materials such as cellulose fiber insulation (CFI), characterized by high water capacity, are a potential countermeasure against such condensation. In this study, the humidity control performance of external walls containing CFI was evaluated using data measured inside a demonstration house and calculated by numerical simulations based on thermodynamic chemical potential theory. The changes in moisture adsorption and desorption were then evaluated for different wall constructions and different climate conditions using a parameter sensitivity analysis. Finally, the effective application of CFI to prevent interstitial condensation was confirmed by comparing different wall compositions.
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http://dx.doi.org/10.1111/ina.12622DOI Listing
March 2020

2-Methylthio Conversion of N6-Isopentenyladenosine in Mitochondrial tRNAs by CDK5RAP1 Promotes the Maintenance of Glioma-Initiating Cells.

iScience 2019 Nov 8;21:42-56. Epub 2019 Oct 8.

Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan; Center for Metabolic Regulation of Healthy Aging, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan; Neutron Therapy Research Center, Okayama University, Okayama 700-8558, Japan. Electronic address:

2-Methylthio-N-isopentenyl modification of adenosine (msiA) is an evolutionally conserved modification found in mitochondrial (mt)-tRNAs. Cdk5 regulatory subunit-associated protein 1 (CDK5RAP1) specifically converts N6-isopentenyladenosine (iA) to msiA at position A37 of four mt-DNA-encoded tRNAs, and the modification regulates efficient mitochondrial translation and energy metabolism in mammals. Here, we report that the ms conversion mediated by CDK5RAP1 in mt-tRNAs is required to sustain glioma-initiating cell (GIC)-related traits. CDK5RAP1 maintained the self-renewal capacity, undifferentiated state, and tumorigenic potential of GICs. This regulation was not related to the translational control of mt-proteins. CDK5RAP1 abrogated the antitumor effect of iA by converting iA to msiA and protected GICs from excessive autophagy triggered by iA. The elevated activity of CDK5RAP1 contributed to the amelioration of the tumor-suppressive effect of iA and promoted GIC maintenance. This work demonstrates that CDK5RAP1 is crucial for the detoxification of endogenous iA and that GICs readily utilize this mechanism for survival.
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http://dx.doi.org/10.1016/j.isci.2019.10.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820277PMC
November 2019

Light-inducible carotenoid production controlled by a MarR-type regulator in Corynebacterium glutamicum.

Sci Rep 2019 09 11;9(1):13136. Epub 2019 Sep 11.

Life Science Research Center, College of Bioresource Sciences, Nihon University, Fujisawa, 252-0880, Japan.

Carotenoid production in some non-phototropic bacteria occurs in a light-dependent manner to protect cells from photo-oxidants. Knowledge regarding the transcriptional regulator involved in the light-dependent production of carotenoids of non-phototrophic bacteria has been mainly confined to coenzyme B-based photo-sensitive regulator CarH/LitR family proteins belonging to a MerR family transcriptional regulator. In this study, we found that bacteria belonging to Micrococcales and Corynebacteriales exhibit light-dependent carotenoid-like pigment production including an amino acid-producer Corynebacterium glutamicum AJ1511. CrtR is a putative MarR family transcriptional regulator located in the divergent region of a carotenoid biosynthesis gene cluster in the genome of those bacteria. A null mutant for crtR of C. glutamicum AJ1511 exhibited constitutive production of carotenoids independent of light. A complemented strain of the crtR mutant produced carotenoids in a light-dependent manner. Transcriptional analysis revealed that the expression of carotenoid biosynthesis genes is regulated in a light-dependent manner in the wild type, while the transcription was upregulated in the crtR mutant irrespective of light. In vitro experiments demonstrated that a recombinant CrtR protein binds to the specific sequences within the intergenic region of crtR and crtE, which corresponds to -58 to -7 for crtE, and +26 to -28 for crtR with respect to the transcriptional start site, and serves as a repressor for crtE transcription directed by RNA polymerase containing SigA. Taken together, the results indicate that CrtR light-dependently controls the expression of the carotenoid gene cluster in C. glutamicum and probably closely related Actinobacteria.
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http://dx.doi.org/10.1038/s41598-019-49384-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739363PMC
September 2019

Solving the Thermoelectric Trade-Off Problem with Metallic Carbon Nanotubes.

Nano Lett 2019 10 9;19(10):7370-7376. Epub 2019 Sep 9.

Department of Physics , Tokyo Metropolitan University , Tokyo 192-0372 , Japan.

Semiconductors are generally considered far superior to metals as thermoelectric materials because of their much larger Seebeck coefficients (). However, a maximum value of in a semiconductor is normally accompanied by a minuscule electrical conductivity (σ), and hence, the thermoelectric power factor ( = σ) remains small. An attempt to increase σ by increasing the Fermi energy (), on the other hand, decreases . This trade-off between and σ is a well-known dilemma in developing high-performance thermoelectric devices based on semiconductors. Here, we show that the use of metallic carbon nanotubes (CNTs) with tunable solves this long-standing problem, demonstrating a higher thermoelectric performance than semiconducting CNTs. We studied the dependence of , σ, and in a series of CNT films with systematically varied metallic CNT contents. In purely metallic CNT films, both and σ monotonically increased with , continuously boosting while increasing . Particularly, in an aligned metallic CNT film, the maximum of was ∼5 times larger than that in the highest-purity (>99%) single-chirality semiconducting CNT film. We attribute these superior thermoelectric properties of metallic CNTs to the simultaneously enhanced and σ of one-dimensional conduction electrons near the first van Hove singularity.
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http://dx.doi.org/10.1021/acs.nanolett.9b03022DOI Listing
October 2019

In vitro evaluation of radiopacity of contrast-loaded superabsorbent polymer microspheres (SAP-MS) with static imaging and flow model.

Jpn J Radiol 2019 Oct 6;37(10):710-718. Epub 2019 Sep 6.

Department of Radiology, Aichi Medical University, 1-1 Yazako Karimata, Nagakute, Aichi, 480-1195, Japan.

Purpose: To evaluate the radiopacity of contrast-loaded superabsorbent polymer microspheres (SAP-MS) under X-ray imaging.

Materials And Methods: SAP-MS were suspended in contrast material (iodixanol) and the diameter change was assessed. The diameter of contrast-loaded SAP-MS in saline was measured sequentially. Radiography of the contrast-loaded SAP-MS was evaluated as radiopacity by contrast-to-noise ratio and visibility by multiple reader scoring. Under digital subtraction angiography, contrast-loaded SAP-MS were injected into a flow model. The flow speed was 1-10 cm/s, and images were acquired at 1-7.5 frames per second using a pulse width of 10-85 ms. Images were assessed by multiple reader scoring.

Results: The diameter of SAP-MS increased to 4.0-5.0 times its original size for approximately 15 min. The diameter of contrast-loaded SAP-MS in saline further increased by 10-30% within several minutes and returned to the previous size. Radiopacity and visibility of contrast-loaded SAP-MS decreased in 30 min after mixing with saline. Visibility was better with slow flow speed and narrow pulse width.

Conclusion: For effective observation, contrast-loaded SAP-MS should be kept in non-diluted contrast material until use. The conditions of slower flow and use of a narrow pulse width are recommended.
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http://dx.doi.org/10.1007/s11604-019-00863-xDOI Listing
October 2019
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