Publications by authors named "Takachika Azuma"

48 Publications

Three-dimensional structure of a high affinity anti-(4-hydroxy-3-nitrophenyl)acetyl antibody possessing a glycine residue at position 95 of the heavy chain.

Authors:
Akihiro Nishiguchi Nobutaka Numoto Nobutoshi Ito Takachika Azuma Masayuki Oda

Mol Immunol 2019 10 11;114:545-552. Epub 2019 Sep 11.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, 606-8522, Japan. Electronic address:

View Article and Find Full Text PDF
October 2019

High-affinity IgM memory B cells are defective in differentiation into IgM antibody-secreting cells by re-stimulation with a T cell-dependent antigen.

Authors:
Yasuyuki Tashiro Akikazu Murakami Yasushi Hara Takeyuki Shimizu Masato Kubo Ryo Goitsuka Hidehiro Kishimoto Takachika Azuma

Sci Rep 2018 09 28;8(1):14559. Epub 2018 Sep 28.

Division of Biosignaling, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba, Japan.

View Article and Find Full Text PDF
September 2018

Pronounced effect of hapten binding on thermal stability of an anti-(4-hydroxy-3-nitrophenyl)acetyl antibody possessing a glycine residue at position 95 of the heavy chain.

Authors:
Yusui Sato Satomi Inaba Harumi Fukada Takachika Azuma Masayuki Oda

Mol Immunol 2017 05 27;85:130-136. Epub 2017 Feb 27.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, Kyoto 606-8522, Japan. Electronic address:

View Article and Find Full Text PDF
May 2017

Somatic hypermutation of immunoglobulin genes in Rad18 knockout mice.

Authors:
Takeyuki Shimizu Satoshi Tateishi Yuki Tanoue Takachika Azuma Haruo Ohmori

DNA Repair (Amst) 2017 02 28;50:54-60. Epub 2016 Dec 28.

Departments of Gene Information Analysis, Institute for Virus Research, Kyoto University, Shogoin Kawara-cho 53, Sakyo-ku, Kyoto 606-8507, Japan.

View Article and Find Full Text PDF
February 2017

Structural dynamics of a single-chain Fv antibody against (4-hydroxy-3-nitrophenyl)acetyl.

Authors:
Yusui Sato Yusuke Tanaka Satomi Inaba Hiroshi Sekiguchi Takahiro Maruno Yuji C Sasaki Harumi Fukada Yuji Kobayashi Takachika Azuma Masayuki Oda

Int J Biol Macromol 2016 Oct 21;91:151-7. Epub 2016 May 21.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan. Electronic address:

View Article and Find Full Text PDF
October 2016

Affinity maturation of anti-(4-hydroxy-3-nitrophenyl)acetyl antibodies accompanies a modulation of antigen specificity.

Authors:
Masayuki Oda Takachika Azuma

Mol Immunol 2016 Feb 11;70:8-12. Epub 2015 Dec 11.

Research Institute for Biological Sciences (RIBS), Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.

View Article and Find Full Text PDF
February 2016

High affinity IgM(+) memory B cells are generated through a germinal center-dependent pathway.

Authors:
Yasushi Hara Yasuyuki Tashiro Akikazu Murakami Miyuki Nishimura Takeyuki Shimizu Masato Kubo Peter D Burrows Takachika Azuma

Mol Immunol 2015 Dec 26;68(2 Pt C):617-27. Epub 2015 Oct 26.

Laboratory for Structural Immunology, Research Institute for Biomedical Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan; Antibody Technology Research Center, Co., Ltd., 2361-1-S401 Yamazaki, Noda, Chiba 278-0022, Japan. Electronic address:

View Article and Find Full Text PDF
December 2015

An asymmetric antibody repertoire is shaped between plasmablasts and plasma cells after secondary immunization with (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin.

Authors:
Yasuyuki Tashiro Akikazu Murakami Ryo Goizuka Takeyuki Shimizu Hidehiro Kishimoto Takachika Azuma

Int Immunol 2015 Dec 7;27(12):609-20. Epub 2015 Jul 7.

Division of Biosignaling, Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba 278-0022, Japan

View Article and Find Full Text PDF
December 2015

Detection and isolation of anti-hapten antibody-secreting cells by cellular affinity matrix technology.

Authors:
Takeyuki Shimizu Takachika Azuma

J Immunol Methods 2015 Jul 17;422:80-6. Epub 2015 Apr 17.

Laboratory of Structural Immunology, Division of Bioinformatics, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science, Yamazaki, Noda, Chiba 278-0022, Japan. Electronic address:

View Article and Find Full Text PDF
July 2015

Generation of a Tlx1(CreER-Venus) knock-in mouse strain for the study of spleen development.

Authors:
Ryo Nakahara Yasuhiro Kawai Akihisa Oda Miyuki Nishimura Akikazu Murakami Takachika Azuma Tomonori Kaifu Ryo Goitsuka

Genesis 2014 Nov 18;52(11):916-23. Epub 2014 Oct 18.

Division of Development and Aging, Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba, Japan.

View Article and Find Full Text PDF
November 2014

Low-affinity IgM antibodies lacking somatic hypermutations are produced in the secondary response of C57BL/6 mice to (4-hydroxy-3-nitrophenyl)acetyl hapten.

Authors:
Akikazu Murakami Hayato Moriyama Mina Osako-Kabasawa Kanako Endo Miyuki Nishimura Keiko Udaka Masamichi Muramatsu Tasuku Honjo Takachika Azuma Takeyuki Shimizu

Int Immunol 2014 Apr 27;26(4):195-208. Epub 2013 Nov 27.

Laboratory of Structural Immunology, Division of Bioinformatics, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science, Chiba 278-0022, Japan.

View Article and Find Full Text PDF
April 2014

Multiarray formation of CHO spheroids cocultured with feeder cells for highly efficient protein production in serum-free medium.

Authors:
Koichi Kutsuzawa Chihiro Takahashi Ryohei Sato Toshihiro Suzuki Hidehiro Kishimoto Akiichi Murakami Takachika Azuma Ryo Abe Hidenori Otsuka

J Nanosci Nanotechnol 2013 Jan;13(1):229-35

Center for Colloid and Interface Science, Research Institute for Science and Technology, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-Ku, Tokyo 162-8601, Japan.

View Article and Find Full Text PDF
January 2013

RBMX: a regulator for maintenance and centromeric protection of sister chromatid cohesion.

Authors:
Sachihiro Matsunaga Hideaki Takata Akihiro Morimoto Kayoko Hayashihara Tsunehito Higashi Kouhei Akatsuchi Eri Mizusawa Mariko Yamakawa Mamoru Ashida Tomoko M Matsunaga Takachika Azuma Susumu Uchiyama Kiichi Fukui

Cell Rep 2012 Apr 22;1(4):299-308. Epub 2012 Mar 22.

Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan.

View Article and Find Full Text PDF
April 2012

In-vitro derived germinal centre B cells differentially generate memory B or plasma cells in vivo.

Authors:
Takuya Nojima Kei Haniuda Tatsuya Moutai Moeko Matsudaira Sho Mizokawa Ikuo Shiratori Takachika Azuma Daisuke Kitamura

Nat Commun 2011 Sep 6;2:465. Epub 2011 Sep 6.

Division of Molecular Biology, Research Institute for Biological Sciences (RIBS), Tokyo University of Science, Noda, Chiba 278-0022, Japan.

View Article and Find Full Text PDF
September 2011

Characterization of memory B cells responsible for affinity maturation of anti- (4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies.

Authors:
Miyuki Nishimura Akikazu Murakami Yasushi Hara Takachika Azuma

Int Immunol 2011 Apr 18;23(4):271-85. Epub 2011 Mar 18.

Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.

View Article and Find Full Text PDF
April 2011

Conformational analysis of human serum albumin and its non-enzymatic glycation products using monoclonal antibodies.

Authors:
Keigo Saito Kuniko Hamano Masatoshi Nakagawa Keiko Yugawa Jin Muraoka Hiroyoshi Kuba Koji Furukawa Takachika Azuma

J Biochem 2011 May 21;149(5):569-80. Epub 2011 Jan 21.

Research Institute for Biological Sciences, Tokyo University of Science, 2669, Yamazaki, Noda, Chiba 278-0022, Japan.

View Article and Find Full Text PDF
May 2011

The amino acid residue at position 95 and the third CDR region in the H chain determine the ceiling affinity and the maturation pathway of an anti-(4-hydroxy-3-nitrophenyl)acetyl antibody.

Authors:
Akikazu Murakami Yuka Takahashi Miyuki Nishimura Takeyuki Shimizu Takachika Azuma

Mol Immunol 2010 Nov-Dec;48(1-3):48-58. Epub 2010 Oct 20.

Research Institute for Biological Sciences (RIBS), Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.

View Article and Find Full Text PDF
January 2011

Evaluation of the conformational equilibrium of reduced hen egg lysozyme by antibodies to the native form.

Authors:
Masayuki Oda Aki Kitai Akikazu Murakami Miyuki Nishimura Takatoshi Ohkuri Yoshito Abe Tadashi Ueda Haruki Nakamura Takachika Azuma

Arch Biochem Biophys 2010 Feb 26;494(2):145-50. Epub 2009 Nov 26.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan.

View Article and Find Full Text PDF
February 2010

Effects of antibody affinity and antigen valence on molecular forms of immune complexes.

Authors:
Masayuki Oda Susumu Uchiyama Masanori Noda Yoshinori Nishi Maiko Koga Kouta Mayanagi Carol V Robinson Kiichi Fukui Yuji Kobayashi Kosuke Morikawa Takachika Azuma

Mol Immunol 2009 Dec 2;47(2-3):357-64. Epub 2009 Oct 2.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan.

View Article and Find Full Text PDF
December 2009

DNA polymerase eta is a limiting factor for A:T mutations in Ig genes and contributes to antibody affinity maturation.

Authors:
Keiji Masuda Rika Ouchida Masayuki Yokoi Fumio Hanaoka Takachika Azuma Ji-Yang Wang

Eur J Immunol 2008 Oct;38(10):2796-805

Laboratory for Immune Diversity, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Japan.

View Article and Find Full Text PDF
October 2008

Anti-peptide antibodies for examining the conformation, molecular assembly and localization of an intracellular protein, ribosomal protein S6, in vivo.

Authors:
Masatoshi Nakagawa Nobuko Ohmido Katsumi Ishikawa Susumu Uchiyama Kiichi Fukui Takachika Azuma

J Biochem 2008 Mar 26;143(3):325-32. Epub 2007 Nov 26.

Division of Biosignaling, Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba, Japan.

View Article and Find Full Text PDF
March 2008

T cells specific to hapten carrier but not to carrier alone assist in the production of anti-hapten and anti-carrier antibodies.

Authors:
Takeyuki Shimizu Yuko Osaka Chihiro Banri-Koike Maiko Yoshida Kanako Endo Koji Furukawa Masayuki Oda Akikazu Murakami Shuhei Ogawa Ryo Abe Takachika Azuma

Int Immunol 2007 Oct 19;19(10):1157-64. Epub 2007 Sep 19.

Division of Structural Immunology, Research Institute for Biological Sciences, Tokyo University of Science, Yamazaki 2669, Noda, Chiba 278-0022, Japan.

View Article and Find Full Text PDF
October 2007

PHB2 protects sister-chromatid cohesion in mitosis.

Authors:
Hideaki Takata Sachihiro Matsunaga Akihiro Morimoto Nan Ma Daisuke Kurihara Rika Ono-Maniwa Masatoshi Nakagawa Takachika Azuma Susumu Uchiyama Kiichi Fukui

Curr Biol 2007 Aug 26;17(15):1356-61. Epub 2007 Jul 26.

Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan.

View Article and Find Full Text PDF
August 2007

Dynamical regulations of protein-ligand bindings at single molecular level.

Authors:
Takuma Sagawa Takachika Azuma Yuji C Sasaki

Biochem Biophys Res Commun 2007 Apr 15;355(3):770-5. Epub 2007 Feb 15.

Sasaki-team, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, 2-20-5, Akebono-cho, Tachikawa, Tokyo 190-0012, Japan.

View Article and Find Full Text PDF
April 2007

Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten.

Authors:
Koji Furukawa Takeyuki Shimizu Akikazu Murakami Ryo Kono Masatoshi Nakagawa Takuma Sagawa Ichiro Yamato Takachika Azuma

Mol Immunol 2007 Mar 21;44(9):2436-45. Epub 2006 Nov 21.

Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

View Article and Find Full Text PDF
March 2007

Regional and segmental flexibility of antibodies in interaction with antigens of different size.

Authors:
Masayuki Oda Susumu Uchiyama Carol V Robinson Kiichi Fukui Yuji Kobayashi Takachika Azuma

FEBS J 2006 Apr;273(7):1476-87

Graduate School of Agriculture, Kyoto Prefectural University, Japan.

View Article and Find Full Text PDF
April 2006

Initial repertoire of anti-(4-hydroxy-3-nitrophenylacetyl) antibodies as potential donors for effective affinity maturation.

Authors:
Koji Furukawa Akio Manabe Atsuko Furukawa Hiroyoshi Kuba Toshihide Okajima Takachika Azuma

Mol Immunol 2006 Apr 9;43(11):1751-60. Epub 2006 Jan 9.

Research Institute for Biological Sciences (RIBS), Tokyo University of Science, 2669, Yamazaki, Noda, Chiba 278-0022, Japan.

View Article and Find Full Text PDF
April 2006

DNA polymerase theta contributes to the generation of C/G mutations during somatic hypermutation of Ig genes.

Authors:
Keiji Masuda Rika Ouchida Arata Takeuchi Takashi Saito Haruhiko Koseki Kiyoko Kawamura Masatoshi Tagawa Takeshi Tokuhisa Takachika Azuma Jiyang O-Wang

Proc Natl Acad Sci U S A 2005 Sep 19;102(39):13986-91. Epub 2005 Sep 19.

Laboratories for Antigen Receptor Diversity, Cell Signaling, and Developmental Genetics, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Kanagawa 230-0045, Japan.

View Article and Find Full Text PDF
September 2005

Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse.

Authors:
Tsunehito Higashi Shuichi Miyakawa Susumu Uchiyama Sachihiro Matsunaga Hideaki Takata Satoru Fujimoto Masanori Noda Akiko Terauchi Takeyuki Shimizu Masayuki Oda Takachika Azuma Kiichi Fukui

J Biotechnol 2005 Nov 19;120(3):262-72. Epub 2005 Sep 19.

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

View Article and Find Full Text PDF
November 2005

Normal immunoglobulin gene somatic hypermutation in Pol kappa-Pol iota double-deficient mice.

Authors:
Takeyuki Shimizu Takachika Azuma Mariko Ishiguro Naoko Kanjo Shuichi Yamada Haruo Ohmori

Immunol Lett 2005 May 15;98(2):259-64. Epub 2004 Dec 15.

Division of Biosignaling, Research Institute for Biological Sciences (RIBS), Tokyo University of Science, Yamazaki 2669, Noda, Chiba 278-0022, Japan.

View Article and Find Full Text PDF
May 2005