Publications by authors named "Takachika Azuma"

48 Publications

Three-dimensional structure of a high affinity anti-(4-hydroxy-3-nitrophenyl)acetyl antibody possessing a glycine residue at position 95 of the heavy chain.

Mol Immunol 2019 10 11;114:545-552. Epub 2019 Sep 11.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, 606-8522, Japan. Electronic address:

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October 2019

High-affinity IgM memory B cells are defective in differentiation into IgM antibody-secreting cells by re-stimulation with a T cell-dependent antigen.

Sci Rep 2018 09 28;8(1):14559. Epub 2018 Sep 28.

Division of Biosignaling, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba, Japan.

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September 2018

Pronounced effect of hapten binding on thermal stability of an anti-(4-hydroxy-3-nitrophenyl)acetyl antibody possessing a glycine residue at position 95 of the heavy chain.

Mol Immunol 2017 05 27;85:130-136. Epub 2017 Feb 27.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, Kyoto 606-8522, Japan. Electronic address:

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May 2017

Somatic hypermutation of immunoglobulin genes in Rad18 knockout mice.

DNA Repair (Amst) 2017 02 28;50:54-60. Epub 2016 Dec 28.

Departments of Gene Information Analysis, Institute for Virus Research, Kyoto University, Shogoin Kawara-cho 53, Sakyo-ku, Kyoto 606-8507, Japan.

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February 2017

Structural dynamics of a single-chain Fv antibody against (4-hydroxy-3-nitrophenyl)acetyl.

Int J Biol Macromol 2016 Oct 21;91:151-7. Epub 2016 May 21.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan. Electronic address:

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October 2016

Affinity maturation of anti-(4-hydroxy-3-nitrophenyl)acetyl antibodies accompanies a modulation of antigen specificity.

Mol Immunol 2016 Feb 11;70:8-12. Epub 2015 Dec 11.

Research Institute for Biological Sciences (RIBS), Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.

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February 2016

High affinity IgM(+) memory B cells are generated through a germinal center-dependent pathway.

Mol Immunol 2015 Dec 26;68(2 Pt C):617-27. Epub 2015 Oct 26.

Laboratory for Structural Immunology, Research Institute for Biomedical Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan; Antibody Technology Research Center, Co., Ltd., 2361-1-S401 Yamazaki, Noda, Chiba 278-0022, Japan. Electronic address:

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December 2015

An asymmetric antibody repertoire is shaped between plasmablasts and plasma cells after secondary immunization with (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin.

Int Immunol 2015 Dec 7;27(12):609-20. Epub 2015 Jul 7.

Division of Biosignaling, Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba 278-0022, Japan

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December 2015

Detection and isolation of anti-hapten antibody-secreting cells by cellular affinity matrix technology.

J Immunol Methods 2015 Jul 17;422:80-6. Epub 2015 Apr 17.

Laboratory of Structural Immunology, Division of Bioinformatics, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science, Yamazaki, Noda, Chiba 278-0022, Japan. Electronic address:

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July 2015

Generation of a Tlx1(CreER-Venus) knock-in mouse strain for the study of spleen development.

Genesis 2014 Nov 18;52(11):916-23. Epub 2014 Oct 18.

Division of Development and Aging, Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba, Japan.

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November 2014

Low-affinity IgM antibodies lacking somatic hypermutations are produced in the secondary response of C57BL/6 mice to (4-hydroxy-3-nitrophenyl)acetyl hapten.

Int Immunol 2014 Apr 27;26(4):195-208. Epub 2013 Nov 27.

Laboratory of Structural Immunology, Division of Bioinformatics, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science, Chiba 278-0022, Japan.

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April 2014

Multiarray formation of CHO spheroids cocultured with feeder cells for highly efficient protein production in serum-free medium.

J Nanosci Nanotechnol 2013 Jan;13(1):229-35

Center for Colloid and Interface Science, Research Institute for Science and Technology, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-Ku, Tokyo 162-8601, Japan.

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January 2013

In-vitro derived germinal centre B cells differentially generate memory B or plasma cells in vivo.

Nat Commun 2011 Sep 6;2:465. Epub 2011 Sep 6.

Division of Molecular Biology, Research Institute for Biological Sciences (RIBS), Tokyo University of Science, Noda, Chiba 278-0022, Japan.

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September 2011

Characterization of memory B cells responsible for affinity maturation of anti- (4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies.

Int Immunol 2011 Apr 18;23(4):271-85. Epub 2011 Mar 18.

Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.

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April 2011

Conformational analysis of human serum albumin and its non-enzymatic glycation products using monoclonal antibodies.

J Biochem 2011 May 21;149(5):569-80. Epub 2011 Jan 21.

Research Institute for Biological Sciences, Tokyo University of Science, 2669, Yamazaki, Noda, Chiba 278-0022, Japan.

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May 2011

The amino acid residue at position 95 and the third CDR region in the H chain determine the ceiling affinity and the maturation pathway of an anti-(4-hydroxy-3-nitrophenyl)acetyl antibody.

Mol Immunol 2010 Nov-Dec;48(1-3):48-58. Epub 2010 Oct 20.

Research Institute for Biological Sciences (RIBS), Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.

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January 2011

Evaluation of the conformational equilibrium of reduced hen egg lysozyme by antibodies to the native form.

Arch Biochem Biophys 2010 Feb 26;494(2):145-50. Epub 2009 Nov 26.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan.

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February 2010

Effects of antibody affinity and antigen valence on molecular forms of immune complexes.

Mol Immunol 2009 Dec 2;47(2-3):357-64. Epub 2009 Oct 2.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan.

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December 2009

DNA polymerase eta is a limiting factor for A:T mutations in Ig genes and contributes to antibody affinity maturation.

Eur J Immunol 2008 Oct;38(10):2796-805

Laboratory for Immune Diversity, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Japan.

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October 2008

Anti-peptide antibodies for examining the conformation, molecular assembly and localization of an intracellular protein, ribosomal protein S6, in vivo.

J Biochem 2008 Mar 26;143(3):325-32. Epub 2007 Nov 26.

Division of Biosignaling, Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba, Japan.

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March 2008

T cells specific to hapten carrier but not to carrier alone assist in the production of anti-hapten and anti-carrier antibodies.

Int Immunol 2007 Oct 19;19(10):1157-64. Epub 2007 Sep 19.

Division of Structural Immunology, Research Institute for Biological Sciences, Tokyo University of Science, Yamazaki 2669, Noda, Chiba 278-0022, Japan.

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October 2007

PHB2 protects sister-chromatid cohesion in mitosis.

Curr Biol 2007 Aug 26;17(15):1356-61. Epub 2007 Jul 26.

Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan.

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August 2007

Dynamical regulations of protein-ligand bindings at single molecular level.

Biochem Biophys Res Commun 2007 Apr 15;355(3):770-5. Epub 2007 Feb 15.

Sasaki-team, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, 2-20-5, Akebono-cho, Tachikawa, Tokyo 190-0012, Japan.

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April 2007

Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten.

Mol Immunol 2007 Mar 21;44(9):2436-45. Epub 2006 Nov 21.

Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

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March 2007

Initial repertoire of anti-(4-hydroxy-3-nitrophenylacetyl) antibodies as potential donors for effective affinity maturation.

Mol Immunol 2006 Apr 9;43(11):1751-60. Epub 2006 Jan 9.

Research Institute for Biological Sciences (RIBS), Tokyo University of Science, 2669, Yamazaki, Noda, Chiba 278-0022, Japan.

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April 2006

DNA polymerase theta contributes to the generation of C/G mutations during somatic hypermutation of Ig genes.

Proc Natl Acad Sci U S A 2005 Sep 19;102(39):13986-91. Epub 2005 Sep 19.

Laboratories for Antigen Receptor Diversity, Cell Signaling, and Developmental Genetics, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Kanagawa 230-0045, Japan.

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September 2005

Normal immunoglobulin gene somatic hypermutation in Pol kappa-Pol iota double-deficient mice.

Immunol Lett 2005 May 15;98(2):259-64. Epub 2004 Dec 15.

Division of Biosignaling, Research Institute for Biological Sciences (RIBS), Tokyo University of Science, Yamazaki 2669, Noda, Chiba 278-0022, Japan.

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May 2005