Publications by authors named "Tai-Jung Tsai"

3 Publications

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Clinical features, microbiological epidemiology and recommendations for management of cellulitis in extremity lymphedema.

J Surg Oncol 2020 Jan 2;121(1):25-36. Epub 2019 Jul 2.

Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan.

Background: This high volume, single center study investigated the prevalence, bacterial epidemiology, and responsiveness to antibiotic therapy of cellulitis in extremity lymphedema.

Methods: From 2003 to 2018, cellulitis events from a cohort of 420 patients with extremity lymphedema were reviewed. Demographics, lymphedema grading, symptoms, inflammatory markers, cultures and antibiotic therapy regimens were compiled from cellulitis episodes data. Univariate and multivariate analyses were performed for detailed analysis.

Results: A total of 131 separate episodes of cellulitis were recorded from 43 (81.1%) lower limb and 10 (19.9%) upper limb lymphedema patients. The prevalence and recurrence rates for cellulitis in lymphedema patients were 12.6% (53 of 420) and 56.6% (30 of 53), respectively. The most common findings were increased limb circumference (127 of 131; 96.9%) and abnormal C-reactive protein (CRP) level (86 of 113; 76.1%). Blood cultures were obtained in 79 (60.3%) incidents, with 9 (11.4%) returning positive. Streptococcus agalactiae was the most isolated bacterium (5 of 9; 55.5%).

Conclusions: The cellulitis prevalence and recurrence rate in extremity lymphedema were 12.6%, and 56.6%, respectively. Strongest indicators of cellulitis were increased affected limb circumference and elevated CRP level. Empiric antibiotic therapy began with coverage for Steptococcus species before broadening to anti-Methicillin-resistant Staphylococcus aureus and anti-Gram negatives if needed for effective treatment of extremity lymphedema cellulitis.
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http://dx.doi.org/10.1002/jso.25525DOI Listing
January 2020

Antitumor properties of Coenzyme Q against human ovarian carcinoma cells via induction of ROS-mediated apoptosis and cytoprotective autophagy.

Sci Rep 2017 08 14;7(1):8062. Epub 2017 Aug 14.

Institute of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, 40402, Taiwan.

Coenzyme Q (CoQ, 2,3-dimethoxy-5-methyl-1,4-benzoquinone) has been reported to exert anticancer properties against human breast/lung cancer cells. This study investigated the in vitro and in vivo anticancer properties of CoQ on human ovarian carcinoma (SKOV-3) cells and xenografted nude mice, and revealed the underlying molecular mechanism. CoQ induced G/M arrest through downregulation of cyclin B1/A and CDK1/K2 expressions. CoQ-induced autophagy as a survival mechanism was evidenced by increased accumulation of LC3-II, GFP-LC3 puncta, AVOs formation and Beclin-1/Bcl-2 dysregulation. Increased TUNEL-positive cells and Annexin-V/PI stained cells indicated CoQ-induced late apoptosis. Both mitochondrial (caspase-3, PARP and Bax/Bcl-2 dysregulation) and ER stress (caspase-12 and Hsp70) signals are involved in execution of apoptosis. Interestingly, CoQ-induced apoptosis/autophagy is associated with suppression of HER-2/neu and PIK/AKT signalling cascades. CoQ triggered intracellular ROS production, whereas antioxidant N-acetylcysteine prevented CoQ-induced apoptosis, but not autophagy. Inhibition of apoptosis by Z-VAD-FMK suppressed CoQ-induced autophagy (diminished LC3-II/AVOs), indicates CoQ-induced apoptosis led to evoke autophagy. Contrary, inhibition of autophagy by 3-MA/CQ potentiated CoQ-induced apoptosis (increased DNA fragmentation/PARP cleavage). Furthermore, CoQ treatment to SKOV-3 xenografted nude mice reduced tumor incidence and burden. Histopathological analyses confirmed that CoQ modulated xenografted tumor progression by apoptosis induction. Our findings emphasize that CoQ triggered ROS-mediated apoptosis and cytoprotective autophagy.
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http://dx.doi.org/10.1038/s41598-017-08659-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556069PMC
August 2017

Influence of Religious Beliefs on the Health of Cancer Patients.

Asian Pac J Cancer Prev 2016 ;17(4):2315-20

Department of Nursing, Chang Gung Memorial Hospital, Taoyuan, Taiwan E-mail :

Background: This study investigated the influence of religious beliefs on the health of cancer patients and identified the factors contributing to the influence.

Materials And Methods: A questionnaire survey was conducted using a convenient sampling method. A structured questionnaire was used to the samplings, and the data of 200 cancer patients were collected.

Results: The effects of religion on the health of cancer patients achieved an average score of 3.58. The top five effects are presented as follows: (a) Religion provides me with mental support and strength, (b) religion enables me to gain confidence in health recovery, (c) religion motivates me to cope with disease-related stress positively and optimistically, (d) religion helps me reduce anxiety, and (e) religion gives me courage to face uncertainties regarding disease progression. Moreover, among the demographic variables, gender, type of religion, and experience of religious miracles contributed to the significantly different effects of religion on patients. Specifically, the effect of religion on the health of patients who were female and Christian and had miracle experiences was significantly (< .01) higher than that on other patients.

Conclusions: These results are helpful in understanding the influence of religious beliefs on the health of cancer patients and identified the factors contributing to the influence. The result can serve as a reference for nursing education and clinical nursing practice.
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http://dx.doi.org/10.7314/apjcp.2016.17.4.2315DOI Listing
January 2017