Publications by authors named "T B Sridharan"

17 Publications

Nanosilver reinforced Parmelia sulcata extract efficiently induces apoptosis and inhibits proliferative signalling in MCF-7 cells.

Environ Res 2021 08 26;199:111375. Epub 2021 May 26.

School of Bioscience and Technology, Vellore Institute of Technology, Vellore, India.

The Lichen, Parmelia sulcata synthesizes various secondary metabolites, in which phenolic based compounds received much attention due to their importance in biomedical application. Especially the phenolic compound was effective against the cancer treatment. An effective administration of such plant natural product can represent a significant conventional management of cancer in terms of chemoprevention. The nanomedicines are group of agents that selectively interfere the cancer cells which leads to reduction of side effect thereby reducing the doses. Silver nanoparticles is a promising antitumor agent, however, the conventional production of silver nanoparticles have many drawbacks which led to increase in need of eco-friendly biological production methods. In this study, we made an attempt to synthesise a nano silver (Ps-AgNPs) from phenolic extract of lichen Parmelia sulcata extract. The Ps-AgNps was applied for anticancer activity using MCF-7 cells and the effect was characterised by western blotting method. The FTIR, XRD, UV and TEM results confirms the presence of silver nanoparticles in phenolic extract of lichen Parmelia sulcata. The cytotoxicity assay shows that the Ps-AgNPs is toxic against cancer cells (MCF-7) but not to normal cells (NIH3T3), which confirm the selective induction of cell death (apoptosis) against cancer cells. The Western blot analysis also clearly indicates the down regulation of inflammatory genes (TNF-alpha and IL-6) and cell cycle genes (PCNA and Cyclin-D1) thus promoting intrinsic apoptotic pathway. The results suggest that Ps-AgNPs can effectively kill cancer cells and can be used as an alternative therapeutic agent for cancer treatment.
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http://dx.doi.org/10.1016/j.envres.2021.111375DOI Listing
August 2021

Oxidative Stress-induced Changes in Fertility Status of Cryopreserved Sperm: a Diagnosis Based on Sperm Chromatin Dispersion Assay.

Cryo Letters 2020 Sep-Oct;41(5):297-302

Department of Biotechnology, School of BioSciences and Technology, VIT, Vellore, Tamil Nadu, India.

Background: Cryopreservation introduces iatrogenic damage to sperm cells due to excess production of reactive oxygen species (ROS) that can damage sperm macromolecules and alter the physiochemical properties of sperm cells. These altered properties can affect the biological potential of sperm cell towards fertility.

Objective: The study was designed to assess the role of oxidative stress in sperm DNA damage upon cryopreservation.

Materials And Methods: Semen samples (160) were classified into fertile and infertile on the basis of Computer Assisted Semen Analysis (CASA), and cryopreserved. Thawed samples were analyzed for 8OHdG marker, sperm chromatin dispersion (SCD)-based DNA fragmentation index (SCD-DFI) and ROS levels. Receiver Operating Characteristics (ROC) was performed to find the specificity and sensitivity of SCD-DFI in assessing the sperm DNA integrity. Principle component analysis (PCA) was performed to group semen parameters.

Results: SCD-DFI significantly correlates with 8OHdG in infertile samples (r=0.73, p<0.0001) and moderately in fertile samples (r=0.52, p<0.001). Among SCD-DFI, ROS and 8OHdG values, SCD-DFI possess a higher area under the curve (0.83) in ROC, suggesting its potential in representing sperm DNA damage. PCA analysis extracted SCD-DFI and sperm progressive motility as two independent parameters, differentiating fertile and infertile cryopreserved semen.

Conclusion: The study proves that SCD can potentially represent ROS-induced sperm DNA damage in infertile semen upon cryopreservation.
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July 2021

Human prostasomes an extracellular vesicle - Biomarkers for male infertility and prostrate cancer: The journey from identification to current knowledge.

Int J Biol Macromol 2020 Mar 12;146:946-958. Epub 2019 Nov 12.

Saveetha School of Engineering, Department of Biomedical Engineering, Saveetha Institute of Medical and Technical Sciences, Saveetha Nagar, Chennai 602 105, India.

Extracellular vesicles (EVs) are gaining attention among the cell biologists and researchers over the last two decades. Prostasomes are considered to be (Evs) secreted by prostate epithelial cells into the semen during emission or ejaculation. Prostasomes contain various proteins required for immune regulation namely, amino and dipeptidyl peptidase; endopeptidase (neutral); decay accelerating factor; angiotensin-converting enzyme. Sperm cells need a few prerequisites in order to fertilize the egg. The role of prostasomes in enhancing the male fertility was reviewed extensively throughout the manuscript. Also, prostasomes have an immunosuppressive, immunomodulatory, antibacterial role in the female reproductive tract, and in some cases they can be used as immunocontraceptive agent to regulate the fertility status. This review will give insights to many active researchers in the field of prostasomal research and male infertility/fertility research. This review will open many unanswered mechanisms of prostasomes with respect to structure-function analysis, fatty acids patterns in diagnosis as well as prognosis of male infertility/fertility. More scientific reports are in need to support the mechanism of prostasomes and its role in immunomodulation. The development of prostasomes as a biomarker for the prostate cancer is still miserable with a lot of controversial results by various researchers.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.09.218DOI Listing
March 2020

Improvements in morphology and membrane stability obtained from -, a cryopreservation medium treated infertile smoker sperm cells - An study.

Toxicol Rep 2019 24;6:889-896. Epub 2019 Aug 24.

Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India.

Background: Plants are the major sources of antioxidants, which maintains oxidant: antioxidant state or is to protect from excessive reactive oxygen species in case of cryo medium selection though maintain sperm stability.

Bottleneck: Cryo preservation of infertile smokers is challengeable with the available medium due to over production of ROS hitches, were cause loss of sperm physiology. Hence, a novel medium is needed to store/protect sperm cells of infertile smokers where they attending/ongoing IVF or oncogenic surgical treatment.

Aim: The aim of this study, is to check the stability of sperm cells by TPP- bark () (E4) cryo medium preserved infertile smoker's against ROS/cryo injury- as a continuous study.

Materials And Methods: 42 infertile smoker's subjects with 28 control subjects were selected. Surface morphology (acrosome) of sperm by scanning electronic microscope, sperm membrane proteins by colorimetric method, sperm head and tail defects by CASA method and finally sperm cell stability is checking its zeta electric potential charges, were all done with E4 cryo medium treated frozen/thawed selected study subjects.

Results: Sperm morphology and zeta potential shows there is no damage along the stability of cells maintained during E4 medium cryopreservation in infertile subjects.

Summary: This is the first study is too established for infertile smokers sperm stability was checked for six months with E4 cryo medium.
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http://dx.doi.org/10.1016/j.toxrep.2019.08.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727244PMC
August 2019

Biosynthesis and statistical optimization of polyhydroxyalkanoate (PHA) produced by Bacillus cereus VIT-SSR1 and fabrication of biopolymer films for sustained drug release.

Int J Biol Macromol 2019 Aug 23;135:945-958. Epub 2019 May 23.

School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India. Electronic address:

Polyhydroxyalkanoates are a fascinating group of biological polymers, which have attracted extensive attention. An effectual PHB accumulating Bacillus cereus strain VIT-SSR1 was isolated from industrial effluent contaminated site. Plackett-Burman design was used to screen the critical factors affecting PHA production. Response surface method was employed to evaluate the interactive effects between the independent variables. The Central composite design was performed to optimize the concentrations of Molasses, ammonium sulphate and initial pH. A PHA content of (1.42 ± 0.01) g/L and a maximum PHA yield of (40.3 ± 0.77) % was obtained on implementing the optimized conditions. The produced biopolymer was analysed using Fourier transform infrared spectroscopy (FT-IR) and Gas chromatography-mass spectrometry analysis(GC-MS) and the thermal properties of the biopolymer were evaluated by Thermogravimetric analysis (TGA), and from the Nuclear magnetic Resonance (NMR) analysis the biopolymer was identified to be polyhydroxybutyrate (PHB). PHB-chitosan blends were prepared and tested for biocompatibility by MTT assay on L929 mouse fibroblast cell lines. Curcumin, a well-known drug was loaded onto PHB-chitosan films and their drug release pattern was observed. Curcumin showed a sustained mode of drug release. PHB films are biocompatible and can act as a potent polymer matrix for drug release studies.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.05.163DOI Listing
August 2019
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