Dr. Syed Rizwan Hussain, PhD - King George's Medical University - Application Scientist

Dr. Syed Rizwan Hussain

PhD

King George's Medical University

Application Scientist

Lucknow, Uttar pradesh | India

Main Specialties: Biochemical Genetics, Oncology

Additional Specialties: Human Clinical cancer genetic


Top Author

Dr. Syed Rizwan Hussain, PhD - King George's Medical University - Application Scientist

Dr. Syed Rizwan Hussain

PhD

Introduction

Master’s degree:
I have trained myself in molecular biology and biochemistry during my Masters degree and various trainings or practical courses attended thereafter. During my M. Sc. Degree, I have done practical courses in cytotoxicity, immunology, various biochemical techniques (ELISA, protein quantification etc.), cell culture systems and drug response.
I revitalized and further explored my practical knowledge during summer training at the National JALMA Institute for Leprosy & Other Mycobacterial Disease, Agra, India. Here, I worked on Development and application of techniques for DNA fingerprinting and Studies on drug resistance using conventional and molecular probes methods.
Afterwards, during Post-graduation, I have done Dissertation on “A study on the oxidative stress in patients suffering with various complications of diabetes mellitus” at the Department of Biochemistry, Era’s Lucknow Medical College and Hospital, Lucknow.

Doctorate degree:
During doctorate degree from 2008- 2013, I worked on genetic basis of various cancers in human beings. My work involved c-KIT proto-oncogene genetic analysis of leukemia (AML, ALL, CML and CLL), breast and prostate cancers cases, especially the individuals with mutations discordance. In my research study, on analysis, the frequency of c-KIT gene mutations was highly significant and showed association with the cases. This could possibly give a genetic pattern of c-KIT mutations in the north Indian population.

As a part of the above PhD work, I analyzed complete exonic regions of c-KIT gene in neoplastic cases. Our rapidly expanding knowledge of the molecular mechanisms of cancer holds great promise for the development of better combined methods of cancer therapy in the near future. It is anticipated that future therapies will likely involve a combination of target-specific agents tailored to particular tumors.

Primary Affiliation: King George's Medical University - Lucknow, Uttar pradesh , India

Specialties:

Additional Specialties:

Research Interests:


View Dr. Syed Rizwan Hussain’s Resume / CV

Education

Nov 2013
Babasaheb Bhimrao Ambedkar University
PhD
The title of my Ph.D. thesis is Role of c-KIT gene mutation(s) in neoplasia of Humans.
Dec 2006
Bundelkhand University
M.Sc.
Biomedical Sciences
Sep 2003
Bokaro Steel City College, Vinoba Bahve University, Hazaribag, Jharkhand, India
B.Sc.
Zoology Hons.
Jun 1999
2 High School Secter-I
Intermediate
Zoology, Botany, Chemistry, Physics

Experience

Apr 2015
Research Scientist
Research and Teaching
DRDO funded grant the project entitled Analysis of Malalignment of lower limb as a risk factor for unorganised physical training and exercises related overuse injuries in young adults
Apr 2015
Research Scientist
Research and Teaching
DRDO funded grant the project entitled Analysis of Malalignment of lower limb as a risk factor for unorganised physical training and exercises related overuse injuries in young adults

Publications

20Publications

235Reads

1062Profile Views

24PubMed Central Citations

Retrospective case-control study of correlation between MTHFR gene and OSCC risk in North India.

Clin Oral Investig 2017 Jul 24;21(6):1929-1934. Epub 2016 Oct 24.

Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, 226 003, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00784-016-1976-zDOI Listing
July 2017
14 Reads
1 Citation
2.352 Impact Factor

Association of (C677T) Gene Polymorphism With Breast Cancer in North India.

Biomark Cancer 2016 27;8:111-117. Epub 2016 Sep 27.

Molecular Cell Biology Laboratory, Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4137/BIC.S40446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040218PMC
September 2016
25 Reads
2 Citations

Association of Interleukin-10 (A1082G) gene polymorphism with Oral squamous cell carcinoma in north Indian population.

J Genet 2016 Jun;95(2):249-55

Molecular Cell Biology Lab, Department of Biochemistry,King George's Medical University, Lucknow 226 003,

View Article

Download full-text PDF

Source
June 2016
29 Reads
1.093 Impact Factor

Role of alpha-crystallin, early-secreted antigenic target 6-kDa protein and culture filtrate protein 10 as novel diagnostic markers in osteoarticular tuberculosis

Nazia Rizvi, Ajai Singh, Manish Yadav, Syed Rizwan Hussain, Salma S, Sabir Ali, Vineet Kumar, Avinash Agarwal.

View Article
March 2016
5 Reads

Analysis of c-KIT gene mutation as a marker for diagnosis of primary osteosarcoma in north Indian population.

International Journal of Recent Scientific Research 2016; 7: 9407-9412.

International Journal of Recent Scientific Research

Background: Osteosarcoma, a frequent tumour of childhood and young adults, represents the most common primary malignant bone tumour. c-KIT gene is expressed in mast cell growth factor, cellular migration, proliferation, melanoblasts, haematopoietic progenitors and germ cells. We have designed our study with aim to explore the significance of c-KIT gene mutation in osteosarcoma patients. Materials and methods: To determine the kind of mutation analysis in exon 9, 11, 13 and 17 of c- KIT gene in 60 osteosarcoma patients. We have done polymerase chain reaction (PCR) -singlestrand conformational polymorphism (SSCP) followed by DNA sequencing. Results: In osteosarcoma cancer the c-KIT gene mutation frequency was 3.33% (02/60) in exon 9, 8.33% (5/60) in exon 11, 15.0% (9/60) in exon 13 and 5.0% (3/60) in exon 17, respectively. We have detected two silent mutations Val497Val and Ile798Ile in exon 9, 17 in five cases and three missense mutations that is Phe584Ser, Lys642Glu and Val654Ala in exon 11, 13 in fourteen cases. The overall c-KIT gene mutation frequency in exons 9, 11, 13 and 17 was determined to be 31.6% (19/60). Conclusions: The c-KIT gene may be used as a molecular prognostic/diagnostic marker of osteosarcoma patients.

View Article
March 2016
6 Reads

Association of Genetic Polymorphism in the Interleukin-8 Gene with Risk of Oral Cancer and Its Correlation with Pain.

Biochem Genet 2016 Feb 10;54(1):95-106. Epub 2015 Dec 10.

Department of Biochemistry, King George's Medical University, Lucknow, UP, 226003, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10528-015-9704-yDOI Listing
February 2016
42 Reads
0.865 Impact Factor

Study of Cysteine-Rich Protein 61 Genetic Polymorphism in Predisposition to Fracture Nonunion: A Case Control.

Genet Res Int 2015 10;2015:754872. Epub 2015 Dec 10.

Molecular Cell Biology Lab, Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh 226 003, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1155/2015/754872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689909PMC
January 2016
16 Reads
2 Citations

The role of P2X7R purinoreceptor in osteosarcoma.

Advances in Modern Oncology Research 2015; 1: 88-96.

Advances in Modern Oncology Research

. Osteosarcoma is the most common type of bone cancer, which appears mainly in the metaphysis of long bones, especially in males between the age of 0–14 years. Malignancy usually emerges with the abnormal growth of tumor-forming bone cells. These tumor cells act like new bones that are responsible for the spread of sarcoma throughout the bone matrix. In this review, we focused on the expression and function of the P2X7 receptor (P2X7R) as a therapeutic target in osteosarcoma malignancy. Two known human P2X7R functional splice variants in osteosarcoma cell growth are the full length P2X7RA and the truncated P2X7RB. The stimulation of growth is attributed to an increase (i) in the mobilization of Ca2+ ions, and (ii) in the nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) activity. Furthermore, Te85 P2X7RA+B cells caused membrane depolarization and spontaneous release of extracellular adeno-sine triphosphate (ATP). The P2X7R agonist, benzoyl adenosine triphosphate (BzATP), may increase the liberation of ATP and this may be regulated by P2X7R. As a result, cell proliferation occurs with the spread of osteosarcoma throughout the bone matrix. BzATP also increases cell growth and activates NFATc1 to make it cancerous. In this re-view, we have highlighted the crucial role of the P2X7R purinoreceptor in osteosarcoma pathogenesis. It is an upstream regulator of all paths that may inhibit the receptor activator of nuclear factor kappa-B ligand (RANKL) and a mechanis-tic target of rapamycin (mTOR) blockers. This review suggests that P2X7R is an attractive therapeutic target for os-teosarcoma.

View Article
November 2015
9 Reads

A study on oncogenic role of Leptin and Leptin receptor in Oral Squamous Cell Carcinoma

Tumor Biology 2015; 36: 6515-6523.

Tumor Biology

Leptin been mainly produced by adipose tissue and cancer cells is the most studied adipokine, amongst the several cytokines. Leptin is an antiapoptotic molecule and inducer of cancer stem cells as well as activates cell proliferation. Its oncogenic, mitogenic, proinflammatory and proangiogenic actions lead to its vital roles in tumourigenesis. Two common functional DNA polymorphisms in the genes of leptin G2548A (LEP) and leptin receptor A668G (LEPR) affect the amount of circulating cytokine-type hormone leptin with risk for development of oral squamous cell carcinoma (OSCC).The present study investigated whether these LEP and LEPR gene polymorphisms are affecting risk for OSCC by comparing the genotypes of patients with controls. A total of 306 OSCC and 228 controls participated in this study. We have determined the frequency of LEP (G2548A) and LEPR (A668G) gene polymorphisms in OSCC cases and controls by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The incidence of leptin gene G2548A homozygous mutant AA polymorphism was significantly increased in the OSCC patients (p=0.002, odds ratio (OR)=2.4, 95 % confidence interval (CI)=1.37–4.22) when compared withcontrols, and leptin receptor A668G homozygous mutant GG polymorphism was significantly high in the OSCC patients as compared to controls (p=0.000, OR=3.8, 95 % CI=1.98–7.62). The polymorphism of homozygous mutant allele A of leptin gene and G allele of leptin receptor may be associated with increased risk for OSCC. The observations showed regular increase of supporting role of leptin in OSCC. The present study showed an association of AA genotype and A allele of LEP G2548A as well as GG genotype and G allele of LEPR A668G polymorphisms with increased risk for OSCC in north Indian patients. Moreover, the combination of both the polymorphisms may be involved in susceptibility and progression of OSCC.

View Article
September 2015
5 Reads

A study on oncogenic role of leptin and leptin receptor in oral squamous cell.

Tumour Biol 2015 Aug 26;36(8):6515-23. Epub 2015 Mar 26.

Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, 226 003, India.

View Article

Download full-text PDF

Source
http://link.springer.com/content/pdf/10.1007/s13277-015-3342
Web Search
http://link.springer.com/10.1007/s13277-015-3342-1
Publisher Site
http://dx.doi.org/10.1007/s13277-015-3342-1DOI Listing
August 2015
9 Reads
2 Citations
2.840 Impact Factor

Association of Interleukin-10 (A1082G) gene polymorphism with Oral squamous cell carcinoma in north Indian population

Journal Of Genetics 2016; 95: 1-8

Journal Of Genetics

The functional polymorphism A1082G in the gene (IL10) for interleukin-10 associated with risk of oral squamous cell carcinoma (OSCC). The present case–control study was to evaluate the possible association between IL10 A1082G gene and OSCC in north Indian population. Analysis of IL10 A1082G genotype in 232 OSCC cases and 221 healthy controls of comparable age, gender, smokers, tobacco chewing and alcohol consumption. IL10 A1082G status in cases and controls were evaluated by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The frequencies of IL10 A1082G polymorphism AA, AG, GG genotypes were 29.74, 68.10 and 2.15% in OSCC cases and 57.46, 42.08 and 0.45% in healthy controls. The average frequency of G mutant allele was 36.20% in OSCC cases compared with 21.50% among the controls and this allele was associated with increased risk for OSCC cases. Heterozygous AG genotype was found statistically significant in OSCC cases than in controls (OR = 1.6, 95% CI = 1.1–2.2, P = 0.003), whereas homozygous mutant GG genotype was not found significant (OR = 4.7, 95% CI = 0.55–41.1, P = 0.2). Moreover, we found that G allele was significant in OSCC cases of tobacco chewing. The frequency of IL10 A1082G polymorphism G allele and AG genotype is associated with OSCC cases as compared with controls; this may be due to smoking and tobacco chewing. Our findings showed that in IL10 A1082G gene polymorphism AG genotype and G allele may participate in the progression of OSCC.

View Article
August 2015
7 Reads

Association of interleukin-6 genetic polymorphisms with risk of OSCC in Indian population.

Meta Gene 2015 Jun 15;4:142-51. Epub 2015 May 15.

Department of Biochemistry, King George's Medical University, UP, Lucknow, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mgene.2015.03.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436510PMC
June 2015
12 Reads
3 Citations

Effects of interleukin-18 promoter (C607A and G137C) gene polymorphisms and their association with oral squamous cell carcinoma (OSCC) in northern India.

Tumour Biol 2014 Dec 15;35(12):12275-84. Epub 2014 Nov 15.

Department of Anaesthesiology, King George's Medical University, Lucknow, Uttar Pradesh, 226003, India.

View Article

Download full-text PDF

Source
http://link.springer.com/content/pdf/10.1007/s13277-014-2538
Web Search
http://link.springer.com/10.1007/s13277-014-2538-0
Publisher Site
http://dx.doi.org/10.1007/s13277-014-2538-0DOI Listing
December 2014
19 Reads
3 Citations
2.840 Impact Factor

Role of 677C→T polymorphism a single substitution in methylenetetrahydrofolate reductase (MTHFR) gene in North Indian infertile men.

Mol Biol Rep 2014 Feb 24;41(2):573-9. Epub 2013 Dec 24.

Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow, 226 003, Uttar Pradesh, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-013-2894-7DOI Listing
February 2014
17 Reads
7 Citations
2.024 Impact Factor

KIT proto-oncogene exon 8 deletions at codon 419 are highly frequent in acute myeloid leukaemia with inv(16) in Indian population.

Mol Biotechnol 2013 Jun;54(2):461-8

Department of Biotechnology, Era's Lucknow Medical College and Hospital, Lucknow, Uttar Pradesh, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12033-012-9584-xDOI Listing
June 2013
6 Reads
2 Citations
1.880 Impact Factor

Screening of the c-kit gene missense mutation in invasive ductal carcinoma of breast among north Indian population.

Mol Biol Rep 2012 Sep 24;39(9):9139-44. Epub 2012 Jun 24.

Department of Biotechnology, Era's Lucknow Medical College and Hospital, Lucknow 226003, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-012-1786-6DOI Listing
September 2012
15 Reads
2.024 Impact Factor

Methylenetetrahydrofolate reductase C677T genetic polymorphisms and risk of leukaemia among the North Indian population.

Cancer Epidemiol 2012 Aug 20;36(4):e227-31. Epub 2012 Mar 20.

Department of Biotechnology, Era's Lucknow Medical College and Hospital, Lucknow 226003, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canep.2012.02.008DOI Listing
August 2012
11 Reads
2 Citations
2.560 Impact Factor

Identification of the c-kit gene mutations in biopsy tissues of mammary gland carcinoma tumor.

J Egypt Natl Canc Inst 2012 Jun 9;24(2):97-103. Epub 2011 Nov 9.

Department of Biochemistry, Era's Lucknow Medical College and Hospital, Lucknow 226003, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jnci.2011.10.003DOI Listing
June 2012
10 Reads

The Frequency of Mutations in Exon 11 of the c-kit Gene in Patients With Leukemia.

Turk J Haematol 2012 Mar 15;29(1):10-6. Epub 2012 Mar 15.

Era's Lucknow Medical College and Hospital, Lucknow, India.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.5505/tjh.2012.60320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986761PMC
March 2012
10 Reads
0.340 Impact Factor