Publications by authors named "Syed Mehmood Ali"

4 Publications

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To study the effect of acute infrared radiation-induced alterations in human skin at cellular and molecular level using in vivo confocal Raman spectroscopy.

Photodermatol Photoimmunol Photomed 2021 Jun 22. Epub 2021 Jun 22.

College of Engineering, Biomedical Engineering Department, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

Background: Solar radiations are classified in terms of wavelengths, including visible light, infrared, and ultraviolet. Infrared radiation (IR) accounts the largest proportion of solar radiations that cause oxidative stress-induced aging of human skin. This study investigates the biochemical changes in proteins, lipids, and DNA associated with acute exposure to IR radiations.

Method: In vivo confocal Raman spectroscopy was used to examine the forearms region of 20 healthy participants with phototype II skin, aged between 18 and 30 years, without IR incidence (T0), with IR incidence 30 minutes (T30) at day 1 and 30 minutes at day 2 (T60). One-way ANOVA and two-tailed t test along with post hoc Bonferroni correction were used to detect the existence of significant differences in the timestamps of stratum corneum, stratum basale, and dermis at all IR wavenumbers under test.

Results: An increase in the Raman peaks of stratum corneum lipids, decrease in stratum basal DNA peaks, and a shift in the amide I peak of collagen in the skin dermis were observed. One-way ANOVA results showed significant differences among timestamps of stratum corneum, stratum basale, and dermis at all wavenumbers under test (P < .001). Furthermore, paired timestamps also showed significant differences (P < .016) except at two wavenumbers 1293 cm and 852 cm in stratum corneum and basale layer clusters on timestamps (T0 & T30 and T30 & T60, P > .016). This study proved that confocal Raman spectroscopy is an useful technique for early evaluation of IR-induced skin changes.
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June 2021

Ultrasonic Synthesis and Biomedical Application of MnZnErYFeO Nanoparticles.

Biomolecules 2021 May 8;11(5). Epub 2021 May 8.

Department of Nano-Medicine Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, 31441 Dammam, Saudi Arabia.

In the present study, biocompatible manganese nanoparticles have been linked with zinc and iron molecules to prepare different derivatives of MnZnErYFeO NPs (x = 0.02, 0.04, 0.06, 0.08, 0.10), using an ultrasonication approach. The structure, surface morphology, and chemical compositions of MnZnErYFeO NPs were elucidated by X-ray diffractometer (XRD), High-resolution transmission electron microscopy (HR-TEM), scanning electron microscope (SEM), and Energy Dispersive X-Ray Analysis (EDX) techniques. The bioactivity of MnZnErYFeO NPs on normal (HEK-293) and (HCT-116) colon cancer cell line was evaluated. The MnZnErYFeO NPs treatment post 48 h resulted in a significant reduction in cells (via MTT assay, having an IC value between 0.88 µg/mL and 2.40 µg/mL). The specificity of MnZnErYFeO NPs were studied by treating them on normal cells line (HEK-293). The results showed that MnZnErYFeO NPs did not incur any effect on HEK-293, which suggests that MnZnErYFeO NPs selectively targeted the colon cancerous cells. Using , antifungal activity was also studied by evaluating minimum inhibitory/fungicidal concentration (MIC/MFC) and the effect of nanomaterial on the germ tube formation, which exhibited that NPs significantly inhibited the growth and germ tube formation. The obtained results hold the potential to design nanoparticles that lead to efficient bioactivity.
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May 2021

In vivo confocal Raman spectroscopic imaging of the human skin extracellular matrix degradation due to accumulated intrinsic and extrinsic aging.

Syed Mehmood Ali

Photodermatol Photoimmunol Photomed 2021 Mar 9;37(2):140-152. Epub 2020 Nov 9.

Department of Biomedical Engineering, College of Engineering, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

Background: Skin aging is a dynamic process that affects the entire body, marked by molecular and structural changes. Type I collagen is the most abundant structural component and accounts 80% of total collagen in human skin. The amount of proline and hydroxyproline reflect the quantity and quality of the collagen fiber in the extracellular matrix of skin, which is alerted due to accumulated effects of intrinsic and extrinsic aging. Extrinsic aging is driven by ultraviolet radiation-induced reactive oxygen species production that activates the matrix metalloproteinase and disrupts the extracellular matrix of skin dermis, while intrinsic aging is the non-enzymatic process resulting in advanced glycation end products (AGEs). In the presence of pentosidine-AGEs, aging process is accelerated.

Method: In vivo Raman spectra of human dermis were collected from forearms of 30 volunteers and were divided into three groups: 10 young adult 25 ± 5 years, 10 old adult 65 ± 10 years and 10 diabetic old adult 65 ± 10 years old male participants. Density functional theory was performed to compute the vibration modes of AGEs, pentosidine, and glucosepane.

Results: In vivo confocal Raman spectroscopy detects the specific changes in the proline and hydroxyproline conformation, collagen fiber degradation of type I collagen and AGE protein contribution to specific Raman bands in the aged dermis because of Intrinsic and Extrinsic aging. Statistical t test marked significant differences (P < .01) in Raman peaks of proline and hydroxyproline among young adult, old adult, and diabetic old adult participants at wavenumbers 855, 875, 922, and 938 cm .

Conclusion: In vivo confocal Raman spectroscopy is a useful tool to detect the AGE markers in the old adult and diabetic old adult male participants, which interacts with the ultraviolet radiations and accelerates the aging process resulting in the extracellular matrix degradation.
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March 2021

An approach to design a wristwatch for the protection of the human skin damage induced by ultraviolet and infrared radiations.

Photodermatol Photoimmunol Photomed 2020 Jul 30;36(4):278-289. Epub 2020 Apr 30.

Department of Public Health, College of Public Health, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

Background: Ultraviolet radiations (UV) absorbed by the skin can drive photochemical reactions which range from sunburn to skin cancer. The repeated exposure to Infrared radiations (IR) induces the heat into the skin, which causes dehydration and erythema as an immediate effect. This heat activates the metalloproteinase enzyme that reduces the number of procollagen and collagen fibers in the dermal skin, which results premature skin aging. This work aims to design a protective measure in order to avoid these damages.

Method: The proposed protective measure is a wristwatch with an alert alarm which can sense UV and IR radiations. Whenever UV/IR radiation levels exceed beyond the defined limits, alarm will be activated that warns the user to apply protective measures. These radiations are detected by SI1145 digital UV Index/IR/visible light sensor and assigned, using Arduino, to an appropriate UV index and IR radiation levels.

Results: The IR and UV readings were recorded several times and at four different hours through the day. The readings showed its highest value at 10 am and 2 pm, which are considered the highest sun intensity. The other readings were at 6 am and 5 pm and considered the least dangerous hours.

Conclusion: The data collected from the sensor are used to program the alarm. To combine all components, a PCB and a prototype were designed and printed. The UV/IR wristwatch is applicable to alert the user from the continuous and accumulated harmful effects of the radiations and enable them to seek protective measures.
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July 2020