Publications by authors named "Swagata Das"

8 Publications

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Lead phytoremediation potentials of four aquatic macrophytes under hydroponic cultivation.

Int J Phytoremediation 2021 8;23(12):1279-1288. Epub 2021 Mar 8.

Aquatic toxicology and Remediation Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, India.

Lead (Pb) is a major toxicological concern of the present day that demands immediate attention. The use of aquatic macrophytes with high Pb tolerance and accumulation may be a very convenient and economically viable solution for remediating Pb. We examined the ability of , and to remove 0.12 mM, 0.24 mM, 0.36 mM, and 0.48 mM Pb for 96-h under hydroponic cultivation system. The plants accumulated variable amounts of Pb: with low mobility of Pb from root to shoot. Lead uptake kinetics were monitored up to 96-h. After 96-h, the uptake efficiency for (98-99%), (79-96%), (45-79%), and (40-76%) was noted. For and an extremely high uptake rate was seen within the initial 24-h of trials, followed by slower uptake till 96-h. and worked best at 0.12 mM Pb. Pb-Phytotoxicity became prominent at 0.48 mM exposure with biomass loss and morphological changes. The plants had a quick growth rate, extensive root system, high biomass yield, and the ability to tolerate and accumulate Pb that made them suitable for phytoremediation purposes. : Lead phytoremediation potential of four aquatic macrophytes found in Indian waters was evaluated. These macrophytes, often considered as weeds, could be used for phytoremediation purposes that would turn out to be a sustainable means of the utilization of natural resources in developing countries like India. In this study, not only metal accumulation by plants but also the lead uptake kinetics at several time intervals and valuable growth attributes were estimated to establish the suitability of these plants as probable lead phytoremediators. Two of the plant species, , and , showed excellent Pb accumulation capacities that had not been reported earlier, to the best of our knowledge. The work is all the more significant as there have been needs for identifying Pb-phytoremediators well suited to native climate and growth conditions that could take up large amounts of metal from the substratum.
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http://dx.doi.org/10.1080/15226514.2021.1895714DOI Listing
September 2021

Vitiligo and Psychiatric Morbidity: A Profile from a Vitiligo Clinic of a Rural-based Tertiary Care Center of Eastern India.

Indian J Dermatol 2018 Jul-Aug;63(4):281-284

Department of Dermatology, Burdwan Medical College, Bardhaman, India.

Background: Vitiligo is an idiopathic acquired progressive de/hypopigmentary disorder of skin and mucosae. In Indian skin depigmentaion is very much obvious and can cause psychological distress, low self esteem and social stigmatization.

Aims: The primary objective of this study was to evaluate the psychiatric morbidity in vitiligo patients and secondary objective was to assess the morbidity in all eight dimensions of psychosocial and physical aspects, i.e. cognitive, social, discomfort, limitations, depression, fear, embarrassment and anger.

Materials And Methods: An institution based case-control study with sixty-one patients of vitiligo and equal number of healthy age and sex matched controls was undertaken. The self-reporting questionnaire-24 (SRQ-24) and skindex (A 61-item survey questionnaire) were used to assess the psychiatric morbidity in both the groups.

Results: The SRQ-assessed psychiatric morbidity in the study group was 63.93%, compared with 24.59% in the control group (<0.0001). Acral vitiligo had maximum association with psychiatric morbidity (86.67%) followed by vitiligo vulgaris (68%), mucosal vitiligo (62.5%) and others. According to the skindex, the most common psychiatric morbidity in vitiligo patients was depression (62.29%) followed by embarrassment (55.73%), social problem (54.09%), cognitive impairment (50.81%), physical limitation (47.54%), discomfort (40.98%), anger (36.06%) and fear (24.59%). The difference in Skindex scoring that marked the psychiatric morbidity among the case and control groups was statistically significant for depression, discomfort, social problem, cognitive impairment, embarrassment (<0.0001) and physical limitation (=0.0044).

Conclusion: Vitiligo has a high degree of psychiatric morbidity.
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http://dx.doi.org/10.4103/ijd.IJD_142_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052755PMC
August 2018

Non diaphanous formin delphilin acts as a barbed end capping protein.

Exp Cell Res 2017 08 17;357(2):163-169. Epub 2017 May 17.

Department of Biological Sciences, Indian Institute of Science Education and Research, Kolkata, India. Electronic address:

Formins are multi domain proteins present ubiquitously in all eukaryotes from lower fungi to higher vertebrates. Formins are characterized by the presence of formin homology domain-2 (FH2) and formin homology domain-1 (FH1). There are fifteen different formins present in mouse and human. Among these metazoan formins, Delphilin is a unique formin having two PDZ domains at the N-terminus and FH1, FH2 domain at the C-terminus respectively. In this study we observed that Delphilin binds to actin filaments, and Delphilin inhibits actin filament elongation like barbed end capping protein CapZ. In vitro, Delphilin stabilized actin filaments by inhibiting actin filament depolymerisation. Therefore, our study demonstrates Delphilin as an actin-filament capping protein.
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http://dx.doi.org/10.1016/j.yexcr.2017.05.014DOI Listing
August 2017

Orientation of tyrosine side chain in neurotoxic Aβ differs in two different secondary structures of the peptide.

R Soc Open Sci 2016 Oct 5;3(10):160112. Epub 2016 Oct 5.

Structural Biology and Bioinformatics Division , Indian Institute of Chemical Biology, Council of Scientific and Industrial Research , 4, Raja S.c. Mullick Road, Kolkata 700032 , India.

Amyloid β (Aβ) peptide is present as a major component in amyloid plaque that is one of the hallmarks of Alzheimer's disease. The peptide contains a single tyrosine residue and Aβ has a major implication in the pathology of the disease progression. Current investigation revealed that the tyrosine side chain attained two different critical stereo orientations in two dissimilar conformational states of the peptide. The extended α-helical structure of the peptide observed in an apolar solvent or methanol/water mixture became disordered in aqueous medium and the radius of gyration decreased. In aqueous medium, the torsional angle around C-C of tyrosine group became -60°. However, in its α-helical conformation in an apolar system, the measured angle was 180° and this rotameric state may be reasoned behind stronger tyrosine fluorescence compared with the disordered state of the peptide. Molecular dynamics simulation analyses and spectroscopic studies have helped us to understand the major structural changes in the secondary structure of the peptide in the two conformational states. A conformational clustering indicated that the compact state is more stable with tyrosine residue attaining the torsion angle value of -60°, whereas the native state (in HFIP/water mixture) is prevalent at a torsion angle value of -180°. High solvent accessibility has possibly stabilized the particular rotameric state (-60°) of the tyrosine residue and could be the reason behind decrease in fluorescence of the sole tyrosine residue in an aqueous buffer solution (pH 7.4) compared with its fluorescence in the α-helical structure in the micellar environment.
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http://dx.doi.org/10.1098/rsos.160112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098961PMC
October 2016

Comparison of dual-antiplatelet therapy durations after endovascular revascularization of infrainguinal arteries.

Ann Vasc Surg 2015 Aug 28;29(6):1235-44. Epub 2015 May 28.

Veteran Affairs North Texas Health Care System, Dallas, TX; University of Texas Southwestern Medical Center, Dallas, TX. Electronic address:

Background: The optimal dual-antiplatelet therapy (DAPT) duration after endovascular revascularization of infrainguinal arteries is uncertain.

Methods: This study examines DAPT prescription trends and 12-month major adverse limb events (MALEs; a composite of repeat endovascular or surgical revascularization, acute vessel thrombosis, or amputation of the target limb), major adverse cardiovascular events (MACEs; all-cause mortality, nonfatal myocardial infarction [MI], stroke, or coronary revascularization), fatal bleeding events, and those requiring interruption or discontinuation of DAPT (hemorrhagic complications) for patients enrolled into the Excellence in Peripheral Artery Disease (XLPAD) registry.

Results: Data on 368 patients prescribed antiplatelet therapy were analyzed; 8.2% were prescribed antiplatelet monotherapy, 48.6% DAPT for ≤3 months, and 43.2% for >3 months. Patients in the >3 DAPT prescribed group were older, had preexisting coronary artery disease (CAD), and prior MI (all P < 0.001). Overall MALE in the ≤3 and >3-month DAPT prescribed groups were 22.3% and 23.9%, respectively (P = 0.541). Survival analysis showed significantly higher rates of MACE in patients prescribed >3-month DAPT (17.6% vs. 9.5%; P = 0.019). An "as-treated" analysis excluded 10 patients who were prescribed DAPT for >3 months and revealed similar rates of MALE (24.9% vs. 20.8%; P = 0.386) and MACE (12.2% vs. 14.8%; P = 0.443) in patients receiving ≤3 and >3 DAPT. Hemorrhagic complications were similar across all prescribed and "as-treated" DAPT groups.

Conclusions: After infrainguinal endovascular procedures, patients with underlying CAD were prescribed longer (>3 months) duration of DAPT and experienced more cardiovascular events compared with those prescribed ≤3 months of DAPT. Adverse limb events were similar in both groups.
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http://dx.doi.org/10.1016/j.avsg.2015.03.040DOI Listing
August 2015

Sequence complexity of amyloidogenic regions in intrinsically disordered human proteins.

PLoS One 2014 3;9(3):e89781. Epub 2014 Mar 3.

Structural Biology and Bioinformatics Division, Council of Scientific and Industrial Research (CSIR)-Indian Institute of Chemical Biology (IICB), Kolkata, India.

An amyloidogenic region (AR) in a protein sequence plays a significant role in protein aggregation and amyloid formation. We have investigated the sequence complexity of AR that is present in intrinsically disordered human proteins. More than 80% human proteins in the disordered protein databases (DisProt+IDEAL) contained one or more ARs. With decrease of protein disorder, AR content in the protein sequence was decreased. A probability density distribution analysis and discrete analysis of AR sequences showed that ∼8% residue in a protein sequence was in AR and the region was in average 8 residues long. The residues in the AR were high in sequence complexity and it seldom overlapped with low complexity regions (LCR), which was largely abundant in disorder proteins. The sequences in the AR showed mixed conformational adaptability towards α-helix, β-sheet/strand and coil conformations.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0089781PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940659PMC
January 2015

A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug-resistant bacteria and Candida.

Biochim Biophys Acta 2013 Feb 29;1828(2):677-86. Epub 2012 Sep 29.

Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.

Lactoferrin (LF) is believed to contribute to the host's defense against microbial infections. This work focuses on the antibacterial and antifungal activities of a designed peptide, L10 (WFRKQLKW) by modifying the first eight N-terminal residues of bovine LF by selective homologous substitution of amino acids on the basis of hydrophobicity, L10 has shown potent antibacterial and antifungal properties against clinically isolated extended spectrum beta lactamases (ESBL), producing gram-negative bacteria as well as Candida strains with minimal inhibitory concentrations (MIC) ranging from 1 to 8 μg/mL and 6.5 μg/mL, respectively. The peptide was found to be least hemolytic at a concentration of 800 μg/mL. Interaction with lipopolysaccharide (LPS) and lipid A (LA) suggests that the peptide targets the membrane of gram-negative bacteria. The membrane interactive nature of the peptide, both antibacterial and antifungal, was further confirmed by visual observations employing electron microscopy. Further analyses, by means of propidium iodide based flow cytometry, also supported the membrane permeabilization of Candida cells. The peptide was also found to possess anti-inflammatory properties, by virtue of its ability to inhibit cyclooxygenase-2 (COX-2). L10 therefore emerges as a potential therapeutic remedial solution for infections caused by ESBL positive, gram-negative bacteria and multidrug-resistant (MDR) fungal strains, on account of its multifunctional activities. This study may open up new approach to develop and design novel antimicrobials.
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http://dx.doi.org/10.1016/j.bbamem.2012.09.021DOI Listing
February 2013

Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel.

Int J Biol Macromol 2011 Jan 1;48(1):38-43. Epub 2010 Oct 1.

Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India.

The Aloe protein of 14 kDa from the Aloe vera leaf gel was isolated by an ion exchange chromatography using DEAE-cellulose and CM-cellulose column. The purified Aloe protein exhibited a potent anti-fungal activity against Candida paraprilosis, Candida krusei and Candida albicans. In addition, the purified Aloe protein also showed an anti-inflammatory property against pure lipoxygenase and cyclooxygenase-2 with 84% and 73% inhibition, respectively, and was verified by binding with these proteins by real time method by the phenomenon of surface plasmon resonance. This Aloe protein is a novel protein possessing antifungal and anti-inflammatory properties and thus sets a platform to be used as a medicinal plant product.
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http://dx.doi.org/10.1016/j.ijbiomac.2010.09.010DOI Listing
January 2011
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