Publications by authors named "Sunita Makchuchit"

8 Publications

  • Page 1 of 1

Anti-Inflammatory Investigations of Extracts of .

Evid Based Complement Alternat Med 2021 6;2021:5512961. Epub 2021 Mar 6.

Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB T6G 2P5, Canada.

has been consumed in the diet in northern Thailand and also used as a medicament in ancient scripture for arthropathies. Thus, this study aimed to evaluate the activity of various extracts from differential parts of via inhibition of inflammatory mediators (NO, TNF-, and PGE) in RAW264.7 macrophages. The chemical composition in active extracts was also analyzed by GC/MS. The parts of this plant studied were whole fruits (F), pericarp (P), and seed (O). The methods of extraction included maceration in hexane, 95% ethanol and 50% ethanol, boiling in water, and water distillation. The results demonstrated that the hexane and 95% ethanolic extract from pericarp (PH and P95) and seed essential oil (SO) were the most active extracts. PH and P95 gave the highest inhibition of NO production with IC as 11.99 ± 1.66 g/ml and 15.33 ± 1.05 g/ml, respectively, and they also showed the highest anti-inflammatory effect on TNF- with IC as 36.08 ± 0.55 g/ml and 34.90 ± 2.58 g/ml, respectively. PH and P95 also showed the highest inhibitory effect on PGE but less than SO with IC as 13.72 ± 0.81 g/ml, 12.26 ± 0.71 g/ml, and 8.61 ± 2.23 g/ml, respectively. 2,3-Pinanediol was the major anti-inflammatory compound analyzed in PH (11.28%) and P95 (19.82%) while terpinen-4-ol constituted a major anti-inflammatory compound in SO at 35.13%. These findings are the first supportive data for ethnomedical use for analgesic and anti-inflammatory activity in acute (SO) and chronic (PH and P95) inflammation.
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http://dx.doi.org/10.1155/2021/5512961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955865PMC
March 2021

Ethnopharmacological analysis from Thai traditional medicine called prasachandaeng remedy as a potential antipyretic drug.

J Ethnopharmacol 2021 Mar 29;268:113520. Epub 2020 Oct 29.

Center of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Thammasat University (Rangsit Campus), Klongluang, Pathumthani, 12120, Thailand; Professor Emeritus, Department of Agricultural Food and Nutritional Science, Faculty of Agricultural Life and Environmental Sciences, University of Alberta, Edmonton, Bualuang ASEAN Chair Professor, Thammasat University, Canada. Electronic address:

Ethnopharmacological Relevance: Prasachandaeng (PSD) remedy is a famous antipyretic drug for adults and children in Thai traditional medicine used and is described in Thailand's National List of Essential Medicine. Relationship between the taste of this herbal medicine, ethnopharmacological used and its pharmacological properties was reviewed.

Aims Of Study: Since there has been no scientific report on the antipyretic activity of PSD, aim of this study was to investigate the efficacy related antipyretic drug of the remedy and its 12 herbal ingredients. It involved quality evaluation of raw materials, extraction of PSD and its ingredients, in vitro evaluation of their inhibitory activities on fever mediators, i.e. NO and PGE production in murine macrophage (RAW 264.7) cell line stimulated by lipopolysaccharide, and its stability study of the 95% ethanolic extract of PSD remedy.

Materials And Methods: PSD remedy was extracted by maceration with 50% and 95% ethanol (PSD50 and PSD95), by decoction with distilled water (PSDW), and hydrolysis of PSDW with 0.1 N HCl (PSDH). The 12 plant ingredients were extracted with 95% ethanol. Quality evaluation of PSD ingredients was performed according to the standard procedures for the quality control of herbal materials. The inhibitory activity on nitric oxide production was determined by the Griess reaction and the inhibition of prostaglandin E production was determined using the ELISA test kit.

Results: PSD ingredients passed the quality standard stipulated for herbal materials. PSD95 exhibited the highest inhibitory activities on the production of NO and PGE with the IC values of 42.40 ± 0.72 and 4.65 ± 0.76 μg/mL, respectively. A standard drug acetaminophen (ACP) exhibited inhibition of NO and PGE production with the IC values of 99.50 ± 0.43 and 6.110 ± 0.661 μg/mL, respectively. The stability study was suggested two years shelf-life of PSD95. This is the first report on the activity related antipyretic activity of PSD remedy and its ingredients against two fever mediators, NO and PGE.

Conclusion: The results suggested that the 95% ethanolic extracts of PSD remedy and some of its ingredients, were better than ACP in reducing fever. PSD should be further studied using in vivo models and clinical trials to support its use as an antipyretic drug in Thai traditional medicine.
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http://dx.doi.org/10.1016/j.jep.2020.113520DOI Listing
March 2021

The anti-allergic and anti-inflammatory effects of Benjakul extract (a Thai traditional medicine), its constituent plants and its some pure constituents using in vitro experiments.

Biomed Pharmacother 2017 May 10;89:1018-1026. Epub 2017 Mar 10.

Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Klongluang, Pathumthani 12120, Thailand; Center of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Thammasat University, Klongluang, Pathumthani 12120, Thailand. Electronic address:

Benjakul (BJK), a Thai traditional medicine preparation, has long been used for balanced health, controlled abnormal of element in the body, carminative, and relief of flatulence. It is composed of five plants: Piper interruptum Opiz., Piper longum L., Piper sarmentosum Roxb., Plumbago indica L., and Zingiber officinale Roscoe. The ethanolic extracts of BJK, its five individual plants, and pure constituents of BJK were investigated for their anti-allergic activity using immunoglobulin E (IgE)-sensitized β-hexosaminidase in the rat basophilic leukemia-2H3 (RBL-2H3) cells and anti-inflammatory activity using lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) in the murine macrophage (RAW 264.7) cells. The ethanolic extracts of BJK showed anti-allergic activity (IC=12.69μg/ml) and exhibited potent NO inhibitory effect (IC=16.60μg/ml), but inactive on TNF-α release. Moreover, 6-shogaol and plumbagin, two pure compounds from BJK, showed higher anti-allergic activity than the ethanolic BJK extract with IC values of 0.28 and 4.03μg/ml, respectively. These compounds were significantly higher than chlorpheniramine (CPM), standard drug, with IC value of 17.98μg/ml. Determination of the anti-inflammatory activity by measuring the inhibition of NO production presented that plumbagin and 6-shogaol exhibited higher than crude BJK extract with IC values of 0.002 and 0.92μg/ml, respectively. In particular, plumbagin also showed higher anti-inflammatory than prednisolone, positive control, with IC value of 0.59μg/ml. 6-Shogaol also showed inhibitory effect on TNF-α release (IC=9.16μg/ml). These preliminary results may provide some scientific support for the use of BJK for the anti-allergic treatment and inflammatory disorders through the inhibition of NO production.
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http://dx.doi.org/10.1016/j.biopha.2017.02.066DOI Listing
May 2017

Anti-allergic, anti-inflammatory and antioxidant activities of the different extracts of Thai traditional remedy called prabchompoothaweep for allergic rhinitis treatment.

J Med Assoc Thai 2014 Aug;97 Suppl 8:S140-8

Background: Prabchompoothaweep remedy (PT) has long been used in Thai traditional medicine to treat allergic rhinitis and asthma. It is composed of 23 plants. It is on National herbal drug list of Thailand, but there is no reportfor anti-allergic, anti-inflammatory and antioxidant activities.

Objective: To investigate anti-allergic, anti-inflammatory and antioxidant activities of the crude extract from PTby different extraction method.

Material And Method: The method of extract used was maceration in 95% ethanol and 50% ethanol; the residue of these extracts were continued extracted by boiling water, they obtained PTE95, PTE50, PTR95 andPTR50, respectively. The other method of extraction was boiling and drying by lyophilizer that obtained PTW Five crude extracts were determined anti- allergic activity by the inhibition of β-hexosaminidase release from RBL-2H3 cell lines, anti-inflammatory activity were determined by the inhibition ofnitric oxide (NO) production from RA W264. 7 cell lines induced by lipopolysaccharide (LPS) and antioxidant activity were tested by DPPH radical scavenging assay.

Results: PTE95 showed the most potent ofanti-allergic activity, anti-inflammatory activity and antioxidant activity (IC5 = 12.97, 22.51 and EC50 = 14.62 μg/ml, respectively).

Conclusion: These results suggest that the method of extraction PT that showed the best anti-allergy, anti-inflamation and antioxidant activity was maceration in 95% ethanol.
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August 2014

Biological activities and chemical content of Sung Yod rice bran oil extracted by expression and soxhlet extraction methods.

J Med Assoc Thai 2014 Aug;97 Suppl 8:S125-32

Background: Sung Yod rice is a red-violet pigmented rice and grown in the southern part of Thailand. Its rice bran oil has attracted the attention ofscientists who have described anti-oxidant properties ofsome ingredients in Sung Yod rice bran oil. Normally, extraction methods ofcommercial product from rice bran oil are by expression or soxhlet extraction with hexane. Thus, biological activities of Sung Yod rice bran oil related to health and chemical content ofrice bran oilfrom the two methods should be studied.

Objective: The objectives of this research were to investigate for biological activities and chemical content ofSung Yod rice bran oil obtainedfrom expression or soxhlet extraction method.

Material And Method: Biological activities such as cytotoxic, anti-inflammatory and antioxidant activities were investigated. Sulphorhodamine (SRB) assay was used to test cytotoxic activity against four human cancer cell lines: lung (COR-L23), cervical (HeLa), prostate (PC-3) and breast (MCF-7) and normal human lung cells (MRC-5). The inhibitory effect on lipopolysaccharide (LPS) induced nitric oxide (NO) production in RA W264. 7 cell lines was usedfor the determination of anti-inflammatory effect. DPPH, TEAC and FRAP assay were carried outfor antioxidant activity. Total phenolic compound was determined by Folin-Ciocalteu reagent. y-oryzanol and vitamin E content were determined by HPLC. Sung Yod rice bran oil was produced by expression method (EX) or by soxhlet extraction method using hexane (SXH-I).

Results: The percentage ofyield ofSung Yod rice bran oil by EX and SXH were 2.16 and 15.23 %w/w, respectively. Only EX showed the selective cytotoxicity against prostate cancer cells (PC-3), (IC50 = 52.06±1.60 μg/ml). It also exhibited high inhibitory effects on NO production (IC50 = 30.09 μg/ml). In contrast, SXH had no anti-inflammatory effect and cytotoxic activity against any of the cancer cells. EXshowed higher antioxidant activity determined using DPPH compared to SXH. It also showed higher amount of yoryzanol and vitamin E than that ofSXH (3.09±1.04 and 1.35±1.56 mg % w/w ofextracts, respectively). Yet, SXH exhibited higher antioxidant power determined by FRAP assay and higher total phenolic content compared with EX CONCLUSION: Sung Yod rice bran oil, produced by expression method, had better benefit for health regarding cytotoxicity against prostate cancer cells (PC-3), anti-inflammatory effect and antioxidant (using DPPH) than that produced by soxhlet method extracted with hexane.
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August 2014

An in vitro inhibitory effect on RAW 264.7 cells by anti-inflammatory compounds from Smilax corbularia Kunth.

Asian Pac J Allergy Immunol 2012 Dec;30(4):268-74

Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Klong Luang, Pathumthani, 12121, Thailand.

Background: Smilax corbularia is a Thai medicinal plant locally known as 'Hua-Khao-Yen Neua', which is used for treating inflammatory conditions.

Objective: To evaluate the anti-inflammatory effect of S. corbularia extracts and its isolated compounds by determination of inhibitory effects on lipopolysaccharide-stimulated PGE2 release, and TNF-alpha and NO production from RAW 264.7 cells.

Methods: The inhibitory effect of aqueous and ethanolic extracts of this plant were determined on LPS-induced NO production, TNF-alpha and PGE2 release in RAW 264.7 cells, as an in vitro indication of possible anti-inflammatory activity. The compounds from active extract were isolated by bioassay-guided fractionation.

Results: Only the ethanolic extract of this plant inhibited TNF-alpha and NO production, with IC50 values of 61.97, and 83.90 microg/ml respectively. Three flavonols, engeletin, astilbin and quercetin were isolated from the ethanolic extract. quercetin possessed the highest inhibitory effect on NO production with IC50 11.2 microg/ml (37.1 microM), whereas engeletin and astilbin had no activity (IC50 >100 microg/ml). All three flavonols possessed potent inhibition of PGE2 release with IC50 values of 14.4, 19.6 and 19.9 microg/ml (33.2, 43.5 and 65.8 microM) respectively. Quercetin also exhibited the highest inhibitory effect on TNF-alpha production (IC50 = 1.25 microg/ml or 4.14 microM), but engeletin and astilbin had no activity.

Conclusion: This is the first report of isolated compounds from S. corbularia with potential anti-inflammatory effects, and the results support the use of this plant by Thai traditional doctors for treatment of inflammatory diseases.
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December 2012

Acetylcholinesterase inhibitory activity of Thai traditional nootropic remedy and its herbal ingredients.

J Med Assoc Thai 2011 Dec;94 Suppl 7:S183-9

Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumtani, Thailand.

The incidence of Alzheimer disease (AD) is increasing every year in accordance with the increasing of elderly population and could pose significant health problems in the future. The use of medicinal plants as an alternative prevention or even for a possible treatment of the AD is, therefore, becoming an interesting research issue. Acetylcholinesterase (AChE) inhibitors are well-known drugs commonly used in the treatment of AD. The aim of the present study was to screen for AChE inhibitory activity of the Thai traditional nootropic recipe and its herbal ingredients. The results showed that ethanolic extracts of four out of twenty-five herbs i.e. Stephania pierrei Diels. Kaempfera parviflora Wall. ex Baker, Stephania venosa (Blume) Spreng, Piper nigrum L at 0.1 mg/mL showed % AChE inhibition of 89, 64, 59, 50; the IC50 were 6, 21, 29, 30 microg/mL respectively. The other herbs as well as combination of the whole recipe had no synergistic inhibitory effect on AChE activity. However some plants revealed antioxidant activity. More research should have be performed on this local wisdom remedy to verify the uses in scientific term.
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December 2011

Antioxidant and nitric oxide inhibition activities of Thai medicinal plants.

J Med Assoc Thai 2010 Dec;93 Suppl 7:S227-35

Graduate School, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.

Nineteen Thai medicinal plants used in Thai traditional medicine preparation to treat colds, asthma and fever were studied for their antioxidant and NO inhibitory activities. Three extracts were obtained from each plant. First extract obtained by macerating the plant part in 95% ethanol (Et) residue was boiled in water, where water extract (EW) was obtained. The third extract (HW) was obtained by boiling each plant in water similar to that of Thai traditional medicine practice. These extracts were tested for their antioxidant activity using DPPH assay, and anti-inflammatory activity by determination of inhibitory activity on lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW 264.7 cell lines using Griess reagent. Results indicated that Et, EW and HW of Syzygium aromaticum showed the highest antioxidant activity (EC50 = 6.56, 4.73 and 5.30 microg/ml, respectively). Et of Atractylodes lancea exhibited the most potent inhibitory activity on lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW 264.7 cells, with IC50 value of 9.70 microg/ml, followed by Et of Angelica sinensis and Cuminum cyminum (IC50 = 12.52 and 13.56 microg/ml, respectively) but water extract (EW, HW) of all plants were apparently inactive. These results of anti-inflammatory activity of these plants correspond with the traditional use for fever; cold, allergic-related diseases and inflammatory-related diseases.
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December 2010