Publications by authors named "Suhua Wang"

130 Publications

Surface-enhanced Raman scattering for mixing state characterization of individual fine particles during a haze episode in Beijing, China.

J Environ Sci (China) 2021 Jun 22;104:216-224. Epub 2020 Dec 22.

MOE Key Laboratory of Resources and Environmental System Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing 102206, China; Guangdong Provincial Key Laboratory of Petrochemcial Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China.

The nondestructive characterization of the mixing state of individual fine particles using the traditional single particle analysis technique remains a challenge. In this study, fine particles were collected during haze events under different pollution levels from September 5 to 11 2017 in Beijing, China. A nondestructive surface-enhanced Raman scattering (SERS) technique was employed to investigate the morphology, chemical composition, and mixing state of the multiple components in the individual fine particles. Optical image and SERS spectral analysis results show that soot existing in the form of opaque material was predominant during clear periods (PM ≤ 75 µg/m). During polluted periods (PM > 75 µg/m), opaque particles mixed with transparent particles (nitrates and sulfates) were generally observed. Direct classical least squares analysis further identified the relative abundances of the three major components of the single particles: soot (69.18%), nitrates (28.71%), and sulfates (2.11%). A negative correlation was observed between the abundance of soot and the mass concentration of PM. Furthermore, mapping analysis revealed that on hazy days, PM existed as a core-shell structure with soot surrounded by nitrates and sulfates. This mixing state analysis method for individual PM particles provides information regarding chemical composition and haze formation mechanisms, and has the potential to facilitate the formulation of haze prevention and control policies.
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http://dx.doi.org/10.1016/j.jes.2020.12.008DOI Listing
June 2021

Effect of photoperiod on vitamin E and carotenoid biosynthesis in mung bean (Vigna radiata) sprouts.

Food Chem 2021 Oct 21;358:129915. Epub 2021 Apr 21.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:

Light affects the accumulation of vitamin E and carotenoids in many crops. This study investigated the impact of photoperiods on the metabolic regulation of vitamin E and carotenoids in mung bean sprouts considering their dietary health benefits. Mung beans were germinated under three different photoperiods: constant light, semilight and constant dark. Results revealed that the semilight photoperiod was optimum for vitamin E and carotenoid accumulation in mung bean sprouts. DXS was activated in the constant dark and was inhibited by constant light. GGPPS and HPT were sensitive to semilight photoperiod in the vitamin E biosynthetic pathway, playing dominant roles in vitamin E accumulation. The PSY, LCYE, LUT5, LUT1 and ZE genes, which are associated with carotenoid biosynthesis, were activated under semilight treatment and significantly regulated the accumulation of carotenoids. This knowledge improves knowledge on light-mediated regulation of vitamin E and carotenoids in mung bean sprouts.
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http://dx.doi.org/10.1016/j.foodchem.2021.129915DOI Listing
October 2021

PXR-mediated expression of FABP4 promotes valproate-induced lipid accumulation in HepG2 cells.

Toxicol Lett 2021 Aug 24;346:47-56. Epub 2021 Apr 24.

Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China; Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou University, Zhengzhou, 450052, China. Electronic address:

Valproate (valproic acid, VPA) is widely used in the therapy of epilepsy. However, adverse effect like hepatic steatosis has been reported in patients receiving VPA treatment. But whether nuclear receptor pregnane X receptor (PXR) and fatty acid binding protein 4 (FABP4) are involved in the regulation of VPA-induced steatosis or not is still unknown. In this study, the roles of PXR and FABP4 in VPA-induced lipid accumulation in HepG2 cells were investigated. We found that the expression of PXR and FABP4 were increased by VPA in a dose-dependent manner. Knockdown of PXR not only reduced lipid accumulation but also impaired the induction of FABP4 by VPA. While overexpression of PXR enhanced both lipid accumulation and FABP4 expression. Moreover, exogenous expression of FABP4 increased triglyceride levels and enhanced lipid accumulation caused by VPA. Taken together, these results suggest that PXR-mediated expression of FABP4 is responsible for lipid accumulation caused by VPA.
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http://dx.doi.org/10.1016/j.toxlet.2021.04.016DOI Listing
August 2021

Behavioral intention of receiving COVID-19 vaccination, social media exposures, and peer discussions in China.

Epidemiol Infect 2021 Apr 23:1-33. Epub 2021 Apr 23.

Centre for Health Behaviours Research, JC School of Public Health and Primary Care, The Chinese University of Hong Kong, New Territory, Hong Kong SAR, China.

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http://dx.doi.org/10.1017/S0950268821000947DOI Listing
April 2021

Factors Influencing Depression and Mental Distress Related to COVID-19 Among University Students in China: Online Cross-sectional Mediation Study.

JMIR Ment Health 2021 Feb 22;8(2):e22705. Epub 2021 Feb 22.

Centre for Health Behaviours Research, Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, Hong Kong.

Background: The COVID-19 epidemic may elevate mental distress and depressive symptoms in various populations in China.

Objective: This study investigates the levels of depression and mental distress due to COVID-19, and the associations between cognitive, behavioral, and psychosocial factors, and depression and mental distress due to COVID-19 among university students in China.

Methods: A large-scale online cross-sectional study (16 cities in 13 provinces) was conducted among university students from February 1 to 10, 2020, in China; 23,863 valid questionnaires were returned. The Patient Health Questionnaire-9 was used to assess depression. Structural equation modeling was performed to test mediation and suppression effects.

Results: Of the 23,863 participants, 47.1% (n=11,235) reported high or very high levels of one or more types of mental distress due to COVID-19; 39.1% (n=9326) showed mild to severe depression. Mental distress due to COVID-19 was positively associated with depression. All but one factor (perceived infection risks, perceived chance of controlling the epidemic, staying at home, contacted people from Wuhan, and perceived discrimination) were significantly associated with mental distress due to COVID-19 and depression. Mental distress due to COVID-19 partially mediated and suppressed the associations between some of the studied factors and depression (effect size of 6.0%-79.5%).

Conclusions: Both mental distress due to COVID-19 and depression were prevalent among university students in China; the former may have increased the prevalence of the latter. The studied cognitive, behavioral, and psychosocial factors related to COVID-19 may directly or indirectly (via mental distress due to COVID-19) affect depression. Interventions to modify such factors may reduce mental distress and depressive symptoms during the COVID-19 epidemic.
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http://dx.doi.org/10.2196/22705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901598PMC
February 2021

Facile mitochondria localized fluorescent probe for viscosity detection in living cells.

Talanta 2021 Apr 22;225:121996. Epub 2020 Dec 22.

Department of Food Science and Technology, National University of Singapore, 3 Science Drive 2, 117542, Singapore. Electronic address:

Fluorescent probes act as a powerful tool to understand the function of intracellular viscosity, which are closely associated with many functional disorders and diseases. Herein we report a boron-dipyrromethene (4,4-difluoro-4-borata-3a,4a-diaza-s-indacene, BODIPY) group based new fluorescent probe (BV-1), which was synthesized facilely by a one-step Knoevenagel-type condensation reaction, to detect viscosity in living cells with high selectivity and sensitivity. DFT calculation demonstrated that the unsaturated moiety at the meso-position of BODIPY suppressed the fluorescence via twisted intramolecular charge transfer (TICT) mechanism in low viscosity media. By restricting the rotation of the molecular rotor, the fluorescence would be enhanced significantly with redshift in emission wavelength in high viscosity conditions. The fluorescence intensity ratio (log (I/I)) at 570 nm showed a good linearity (R = 0.991) with the viscosity (log η) in the range of 2-868 cP. And the limit of detection (LOD) and limit of quantification (LOQ) for viscosity were calculated to be 0.16 cP and 0.54 cP, respectively. BV-1 was demonstrated to be mitochondria localized with low cytotoxicity. Utilizing the new probe BV-1, the changes in mitochondrial viscosity caused by monensin or nystatin have been monitored successfully in real time. This work will provide new efficient ways for the development of viscosity probes, which are expected to be used for the study of intracellular viscosity properties and functions.
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http://dx.doi.org/10.1016/j.talanta.2020.121996DOI Listing
April 2021

Intention to Receive the COVID-19 Vaccination in China: Application of the Diffusion of Innovations Theory and the Moderating Role of Openness to Experience.

Vaccines (Basel) 2021 Feb 5;9(2). Epub 2021 Feb 5.

Centre for Health Behaviours Research, JC School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, China.

COVID-19 has caused a devastating impact on public health and made the development of the COVID-19 vaccination a top priority. Herd immunity through vaccination requires a sufficient number of the population to be vaccinated. Research on factors that promote intention to receive the COVID-19 vaccination is warranted. Based on Diffusion of Innovations Theory, this study examines the association between the perceived efficacy of the COVID-19 vaccination, use of social media for COVID-19 vaccine-related information, openness to experience and descriptive norm with the intention to receive the COVID-19 vaccination, and the moderating role of openness to experience among 6922 university students in mainland China. The intention to receive the free and self-paid COVID-19 vaccination is 78.9% and 60.2%, respectively. Results from path analyses show that perceived efficacy of the COVID-19 vaccination, use of social media for COVID-19 vaccine-related information, and openness to experience and descriptive norm are all positively associated with the intention to receive COVID-19 free and self-paid vaccination. The association between the perceived efficacy of the COVID-19 vaccination and descriptive norm with the intention to receive the COVID-19 vaccination is stronger among those with a lower level of openness to experience. Our findings support the usefulness of Diffusion of Innovations Theory and the moderating role of openness of experience in explaining intention to receive the COVID-19 vaccination.
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http://dx.doi.org/10.3390/vaccines9020129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915878PMC
February 2021

Nitrogen doped carbon spheres with wrinkled cages for the selective oxidation of 5-hydroxymethylfurfural to 5-formyl-2-furancarboxylic acid.

Chem Commun (Camb) 2021 Feb 2;57(16):2005-2008. Epub 2021 Feb 2.

College of Chemistry, Guangdong University of Petrochemical Technology, Maoming 525000, P. R. China.

Nitrogen doped carbon spheres with wrinkled cages (NCSWCs), which were used for the first time as metal-free catalysts, exhibited high catalytic activity and selectivity in the oxidation of 5-hydroxymethylfurfural (HMF) to 5-formyl-2-furancarboxylic acid (FFCA) under base-free conditions using tert-butyl hydroperoxide (TBHP) as the oxidant. The mechanistic studies found that this reaction was catalyzed by the synergy between NCSWCs and TBHP. The density functional theory (DFT) calculations further suggested that the hydroperoxyl radicals from TBHP adsorbed on the carbon atoms adjacent to the graphitic N atoms are the active sites.
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http://dx.doi.org/10.1039/d0cc07856eDOI Listing
February 2021

Acute acrylonitrile exposure inhibits endogenous HS biosynthesis in rat brain and liver: The role of CBS/3-MPST-HS pathway in its astrocytic toxicity.

Toxicology 2021 03 21;451:152685. Epub 2021 Jan 21.

Department of Preventive Medicine and Public Health Laboratory Science, School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212013, China; Center for Experimental Research, Affiliated Kunshan Hospital to Jiangsu University School of Medicine, Kunshan, Suzhou, Jiangsu, 215132, China. Electronic address:

Hydrogen sulfide (HS) as the third gasotransmitter molecule serves various biological regulatory roles in health and disease. Acrylonitrile (AN) is a common occupational toxicant and environmental pollutant, causing brain and liver damage in mammals. The biotransformation of AN is dependent-upon reduced glutathione (GSH), cysteine and other sulfur-containing compounds. However, the effects of AN on the endogenous HS biosynthesis pathway have yet to be determined. Herein, we demonstrated that a single exposure to AN (at 25, 50, or 75 mg/kg for 1, 6 or 24 h) decreased the endogenous HS content and HS-producing capacity in a dose-dependent manner, both in the cerebral cortex and liver of rats in vivo. In addition, the inhibitory effects of AN (1, 2.5, 5, 10 mM for 12 h) on the HS content and/or the expression of HS-producing enzymes were also found both in primary rat astrocytes and rat liver cell line (BRL cells). Impairment in the HS biosynthesis pathway was also assessed in primary rat astrocytes treated with AN. It was found that inhibition of the cystathionine-β-synthase (CBS)/3-mercaptopyruvate sulfurtransferase (3-MPST)-HS pathway with the CBS inhibitor or 3-MPST-targeted siRNA significantly increased the AN-induced (5 mM for 12 h) cytotoxicity in astrocytes. In turn, CBS activation or 3-MPST overexpression as well as exogenous NaHS supplementation significantly attenuated AN-induced cytotoxicity. Taken together, endogenous HS biosynthesis pathway was disrupted in rats acutely exposed to AN, which contributes to acute AN neurotoxicity in primary rat astrocytes.
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http://dx.doi.org/10.1016/j.tox.2021.152685DOI Listing
March 2021

LncRNA loc105377478 promotes NPs-NdO-induced inflammation in human bronchial epithelial cells through the ADIPOR1/NF-κB axis.

Ecotoxicol Environ Saf 2021 Jan 12;208:111609. Epub 2020 Nov 12.

School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, PR China. Electronic address:

With the wide application of neodymium oxide nanoparticles (NPs-NdO) in various fields, their health hazards have aroused public concern in recent years. However, data regarding the cytotoxicity of NPs-NdO is limited. In this study, we investigated the function and mechanism of long-chain non-coding RNAs (lncRNAs) in NPs-NdO-induced airway inflammation. Treatment with NPs-NdO induced an inflammatory response in human bronchial epithelial cells (16HBE) by upregulating the expression of interleukin-6 (IL-6) and interleukin-8 (IL-8). The levels of LDH and intracellular ROS in the cells treated by various doses of NPs-NdO also increased significantly. After treatment with 10 μg/ml NPs-NdO, RNA microarray and real-time quantitative polymerase chain reaction (qRT-PCR) showed a significant upregulation of lncRNA loc105377478. Functional experiments suggested lncRNA loc105377478 enhanced the expression of IL-6, IL-8 and ROS in NPs-NdO-treated 16HBE cells, and it was further demonstrated that lncRNA loc105377478 promoted the activation of NF-κB by negatively regulating ADIPOR1 expression. Moreover, the expression of IL-6 and IL-8 in NPs-NdO-treated 16HBE cells was regulated by lncRNA loc105377478, which was mediated by the NF-κB signaling pathway. In conclusion, lncRNA loc105377478 promotes NF-κB activation by negatively regulating ADIPOR1 expression, thereby upregulating the expression of IL-6 and IL-8 in 16HBE cells treated with NPs-NdO.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111609DOI Listing
January 2021

Construction of a High-Density Genetic Map and Its Application for QTL Mapping of Leaflet Shapes in Mung Bean ( L.).

Front Genet 2020 30;11:1032. Epub 2020 Sep 30.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Mung bean ( L.) is an important but understudied food legume in Asia and now worldwide. Genetic studies may help to accelerate the exploitation of new genes for breeding in this crop. Here, we used a recombination inbred line population to construct an SNP genetic linkage map by genome sequencing technology. We obtained 21,508 high-quality SNP markers integrated into 1,946 bin markers that were mapped onto 11 linkage groups (LGs) with 99-258 bin markers per LG. The total genetic length of the map was 1060.2 cM (38.76-168.03 cM per LG), with an average distance between markers of 0.54 cM. However, there were 18 gaps >5 cM, distribution on LG1, 3, 5, 7, and 9. Gene mapping for lobed and indented leaflets was conducted using the map. A major quantitative trait locus (QTL) associated with indented leaflets was detected on chromosome 10, with phenotypic variation explained (PVE) values of 39.7% and 45.4% under two different environments. Several QTLs for lobed leaflets were detected and most of them were tightly linked together on Chromosome 3. However, only one major QTL, which explained the largest phenotypic variation (27.7-69.5%), was stably detected under two different environments using both R and Q methods. In the two main stable QTLs regions on chromosomes 3 and 10, candidate genes for regulating the molecular mechanism of different leaflet shapes were detected by functional annotation. The overlap of major QTLs under different environments indicated that the present map would be good enough for precisely mapping genes, and both the QTL analysis and gene prediction were useful for investigating the mechanism of leaf development in mung bean or legumes.
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http://dx.doi.org/10.3389/fgene.2020.01032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571465PMC
September 2020

Construction of a High-Density Genetic Map and Its Application for QTL Mapping of Leaflet Shapes in Mung Bean ( L.).

Front Genet 2020 30;11:1032. Epub 2020 Sep 30.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Mung bean ( L.) is an important but understudied food legume in Asia and now worldwide. Genetic studies may help to accelerate the exploitation of new genes for breeding in this crop. Here, we used a recombination inbred line population to construct an SNP genetic linkage map by genome sequencing technology. We obtained 21,508 high-quality SNP markers integrated into 1,946 bin markers that were mapped onto 11 linkage groups (LGs) with 99-258 bin markers per LG. The total genetic length of the map was 1060.2 cM (38.76-168.03 cM per LG), with an average distance between markers of 0.54 cM. However, there were 18 gaps >5 cM, distribution on LG1, 3, 5, 7, and 9. Gene mapping for lobed and indented leaflets was conducted using the map. A major quantitative trait locus (QTL) associated with indented leaflets was detected on chromosome 10, with phenotypic variation explained (PVE) values of 39.7% and 45.4% under two different environments. Several QTLs for lobed leaflets were detected and most of them were tightly linked together on Chromosome 3. However, only one major QTL, which explained the largest phenotypic variation (27.7-69.5%), was stably detected under two different environments using both R and Q methods. In the two main stable QTLs regions on chromosomes 3 and 10, candidate genes for regulating the molecular mechanism of different leaflet shapes were detected by functional annotation. The overlap of major QTLs under different environments indicated that the present map would be good enough for precisely mapping genes, and both the QTL analysis and gene prediction were useful for investigating the mechanism of leaf development in mung bean or legumes.
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http://dx.doi.org/10.3389/fgene.2020.01032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571465PMC
September 2020

Dual-mode immunoassay system based on glucose oxidase-triggered Fenton reaction for qualitative and quantitative detection of danofloxacin in milk.

J Dairy Sci 2020 Sep 26;103(9):7826-7833. Epub 2020 Jun 26.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address:

In this study, a novel colorimetric and fluorescent dual-mode ELISA based on glucose oxidase (GOx)-triggered Fenton reaction was developed for the qualitative and quantitative detection of danofloxacin (DAN). In this system, streptavidin-linked biotinylated anti-DAN-monoclonal antibody (SA-Bio-mAb) and biotinylated GOx (Bio-GOx) form the immune complex mAb-Bio-SA-Bio-GOx. In the absence of DAN, the mAb-Bio-SA-Bio-GOx would be immobilized by combining with coated DAN-BSA and catalyzed glucose to generate HO. The Fenton reaction between HO and Fe generated hydroxyl radicals, which oxidized the o-phenylenediamine to 2,3-diamino-phenazine. A dual-signal immunoassay with colorimetry and fluorescence as the signal readout was established. In the presence of DAN, DAN and DAN-BSA competed with Bio-mAb, decreasing the connection between immune complexes and DAN-BSA and finally resulting in lower signal of colorimetry and fluorescence. Under optimal conditions, the limit of detection of the fluorescence immunoassay was 0.337 ng/mL and was 5.24-fold lower than that of traditional ELISA. The colorimetric immunoassay cut-off value was 30 ng/mL in milk. The average recoveries of the method for milk samples that are spiked with different concentrations of DAN were 91.1 to 128.3%, with a coefficient of variation of 0.7 to 8.2%. These results of the method exhibited good agreement with those of liquid chromatography-tandem mass spectrometry system (LC-MS/MS) method. In brief, this work provides an improved screening strategy with high sensitivity and accuracy for the qualitative or quantitative detection of DAN in milk monitoring.
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http://dx.doi.org/10.3168/jds.2020-18256DOI Listing
September 2020

Glucose oxidase-induced colorimetric immunoassay for qualitative detection of danofloxacin based on iron (Ⅱ) chelation reaction with phenanthroline.

Food Chem 2020 Oct 19;328:127099. Epub 2020 May 19.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address:

In this study, we developed a competitive colorimetric immunoassay for qualitative detection of DAN based on oxidation of iron (Ⅱ) (Fe) in the presence of glucose oxidase (GOx) and color change induced by Fe-phenanthroline (Phen) chromogenic system. Streptavidin (SA) acted as a linker between biotinylated anti-DAN-monoantibody (bio-mAb) and biotinylated GOx (bio-GOx) to form the immunocomplexes bio-mAb-SA-bio-GOx. In the absence of DAN, the immunocomplexes bio-mAb-SA-bio-GOx combining with coated DAN-ovalbumin (DAN-OVA) will be immobilized and catalyze glucose to produce HO. Fe is oxidized to Fe by HO, giving rise to a colorless result. In the presence of DAN, Fe produces a chelation reaction with Phen, leading to orange-red color. Under optimal conditions, the detection limit (LOD) by naked eyes was 2.5 ng mL in milk, chicken, beef, and pork samples. Low LOD, no matrix effect, and no signal reader requirement make it possibly applied to quickly screen DAN on site.
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http://dx.doi.org/10.1016/j.foodchem.2020.127099DOI Listing
October 2020

Differential susceptibility of PC12 and BRL cells and the regulatory role of HIF-1α signaling pathway in response to acute methylmercury exposure under normoxia.

Toxicol Lett 2020 Oct 24;331:82-91. Epub 2020 May 24.

Department of Preventive Medicine and Public Health Laboratory Sciences, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; Center for Experimental Research, Kunshan Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu 215130, China. Electronic address:

Hypoxia-inducible factor 1 (HIF-1) is a critical nuclear transcription factor for adaptation to hypoxia; its regulatable subunit, HIF-1α, is a cytoprotective regulatory factor. We examined the effects of methylmercury (MeHg) in rat adrenal pheochromocytoma (PC12) cells and the rat hepatocyte cell line BRL. MeHg treatment led to time- and concentration-dependent toxicity in both lines with statistically significant cytotoxic effects at 5 μM and 10 μM in PC12 and BRL, respectively, at 0.5 h. HIF-1α protein levels were significantly decreased at 2.5 (PC12) and 5 (BRL) μM MeHg. Furthermore, MeHg reduced the protein levels of HIF-1α and its target genes (glucose transporter-1, vascular endothelial growth factor-A and erythropoietin). Overexpression of HIF-1α significantly attenuated MeHg-induced toxicity in both cell types. Notably, cobalt chloride, a pharmacological inducer of HIF-1α, significantly attenuated MeHg-induced toxicity in BRL but not PC12. In both cell lines, an inhibitor of prolyl hydroxylase, 3, 4-dihydroxybenzoic acid, and the proteasome inhibitor carbobenzoxy-L-leucyl-L-leucyl-L-leucinal(MG132), antagonized MeHg toxicity, while 2-methoxyestradiol, a HIF-1α inhibitor, significantly increased it. These data establish that: (a) neuron-like PC12 cells are more sensitive to MeHg than non-neuronal BRL cells; (b) HIF-1α plays a similar role in MeHg-induced toxicity in both cell lines; and (c) upregulation of HIF-1α offers general cytoprotection against MeHg toxicity in PC12 and BRL cell lines.
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http://dx.doi.org/10.1016/j.toxlet.2020.05.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366344PMC
October 2020

Synthesis and fabrication of g-CN-based materials and their application in elimination of pollutants.

Sci Total Environ 2020 Aug 5;731:139054. Epub 2020 May 5.

Hebei Key Lab of Power Plant Flue Gas Multi-Pollutants Control, Department of Environmental Science and Engineering, North China Electric Power University, Baoding 071003, PR China; College of Environmental Science and Engineering, North China Electric Power University, Beijing 102206, PR China. Electronic address:

With the fast development of industrial and human activity, large amounts of persistent organic pollutants, heavy metal ions and radionuclides are released into the natural environment, which results in environmental pollution. The efficient elimination of the natural environment is crucial for the protection of environment to against the pollutants' toxicity to human beings and living organisms. Graphitic carbon nitride (g-CN) has drawn multidisciplinary attention especially in environmental pollutants' cleanup due to its special physicochemical properties. In this review, we summarized the recent works about the synthesis of g-CN, element-doping, structure modification of g-CN and g-CN-based materials, and their application in the sorption, photocatalytic degradation and reduction-solidification of persistent organic pollutants and heavy metal ions. The interaction mechanisms were discussed from advanced spectroscopic analysis and computational approaches at molecular level. The challenges and future perspectives of g-CN-based materials' application in environmental pollution management are presented in the end. This review highlights the real applications of g-CN-based materials as adsorbents or photocatalysts in the adsorption-reduction-solidification of metal ions or photocatalytic degradation of organic pollutants. The contents are helpful for the undergraduate students to understand the recent works in the elimination of organic/inorganic pollutants in their pollution management.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139054DOI Listing
August 2020

Phosphorylation-Dependent SERS Readout for Activity Assay of Protein Kinase A in Cell Extracts.

Nanomaterials (Basel) 2020 Mar 22;10(3). Epub 2020 Mar 22.

Key Laboratory of Biomimetic Sensor and Detecting Technology of Anhui Province, School of Materials and Chemical Engineering, West Anhui University, Lu'an 237012, Anhui, China.

Protein kinases are key regulators of cell function, the abnormal activity of which may induce several human diseases, including cancers. Therefore, it is of great significance to develop a sensitive and reliable method for assaying protein kinase activities in real biological samples. Here, we report the phosphorylation-dependent surface-enhanced Raman scattering (SERS) readout of spermine-functionalized silver nanoparticles (AgNPs) for protein kinase A (PKA) activity assay in cell extracts. In this assay, the presence of PKA would phosphorylate and alter the net charge states of Raman dye-labeled substrate peptides, and the resulting anionic products could absorb onto the AgNPs with cationic surface charge through electrostatic attraction. Meanwhile, the Raman signals of dyes labeled on peptides were strongly enhanced by the aggregated AgNPs with interparticle hot spots formed in assay buffer. The SERS readout was directly proportional to the PKA activity in a wide range of 0.0001-0.5 U·μL with a detection limit as low as 0.00003 U·μL. Moreover, the proposed SERS-based assay for the PKA activity was successfully applied to monitoring the activity and inhibition of PKA in real biological samples, particularly in cell extracts, which would be beneficial for kinase-related disease diagnostics and inhibitor screening.
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http://dx.doi.org/10.3390/nano10030575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153394PMC
March 2020

Calcium Ions Turn on the Fluorescence of Oxytetracycline for Sensitive and Selective Detection.

J Fluoresc 2020 May 6;30(3):463-470. Epub 2020 Mar 6.

MOE Key Laboratory of Resources and Environmental System Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing, 102206, People's Republic of China.

Herein, we report an interesting finding about the new application of oxytetracycline (OTC), as a fluorescent probe for the detection of calcium ion (Ca), which proved that it can offer an expeditious, highly sensitive, and selective detection method for Ca. Upon the addition of Ca, the fluorescence of OTC could be significantly enhanced with rapid response and high sensitivity, and achieved a good limit of detection as low as 125 nM in aqueous solution. The complex formed via Ca coordinating to the hydroxyl group of OTC contributes to the fluorescence enhancement, which has been proved by several characterization methods including UV-vis analysis, binding constant determination, and fluorescence titration. The method avoided complexity for EDTA measurement of Ca in running water as proposed previously. Taking advantage of good availability, stability and operability, the OTC was further successfully applied to the detection of Ca in a real environment. Graphical Abstract.
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http://dx.doi.org/10.1007/s10895-020-02512-3DOI Listing
May 2020

Phenotypic comparison and DNA sequencing analysis of a wild-type and a pediocin-resistant mutant of Listeria ivanovii.

Res Microbiol 2020 Apr - Jun;171(3-4):115-121. Epub 2020 Feb 28.

College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin, 300392, People's Republic of China. Electronic address:

Listeria ivanovii is one of the two pathogenic species within the genus Listeria, the other being Listeria monocytogenes. In this study, we generated a stable pediocin resistant mutant Liv-r1 of a L. ivanovii strain, compared phenotypic differences between the wild-type and the mutant, localised the pediocin-induced mutations in the chromosome, and analysed the mechanisms behind the bacteriocin resistance. In addition to pediocin resistance, Liv-r1 was also less sensitive to nisin. The growth of Liv-r1 was significantly reduced with glucose and mannose, but less with cellobiose. The cells of Liv-r1 adsorbed less pediocin than the wild-type cells. Consequently, with less pediocin on the cell surface, the mutant was also less leaky, as shown as the release of intracellular lactate dehydrogenase to the supernatant. The surface of the mutant cells was more hydrophobic than that of the wild-type. Whole genome sequencing revealed numerous changes in the Liv-r1 chromosome. The mutations were found e.g., in genes encoding sigma-54-dependent transcription regulator and internalin B, as well as in genes involved in metabolism of carbohydrates such as glucose and cellobiose. Genetic differences observed in the mutant may be responsible for resistance to pediocin but no direct evidence is provided.
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http://dx.doi.org/10.1016/j.resmic.2020.02.004DOI Listing
November 2020

Verification of Bacteroidales 16S rRNA markers as a complementary tool for detecting swine fecal pollution in the Yangtze Delta.

J Environ Sci (China) 2020 Apr 9;90:59-66. Epub 2019 Dec 9.

Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou 310016, China. Electronic address:

To correctly assess and properly manage the public health risks associated with exposure to contaminated water, it is necessary to identify the source of fecal pollution in a watershed. In this study, we evaluated the efficacy of our two previously developed real time-quantitative PCR (qPCR) assays for the detection of swine-associated Bacteroidales genetic markers (gene 1-38, gene 3-53) in the Yangtze Delta watershed of southeastern China. The results indicated that the gene 1-38 and 3-53 markers exhibited high accuracy (92.5%, 91.7% conditional probability, respectively) in detecting Bacteroidales spp. in water samples. According to binary logistic regression (BLR), these two swine-associated markers were well correlated (P < 0.05) with fecal indicators (Escherichia coli and Enterococci spp.) and zoonotic pathogens (E. coli O157: H7, Salmonella spp. and Campylobacter spp.) in water samples. In contrast, concentrations of conventional fecal indicator bacteria (FIB) were not correlated with zoonotic pathogens, suggesting that they are noneffective at detecting fecal pollution events. Collectively, the results obtained in this study demonstrated that a swine-targeted qPCR assay based on two Bacteroidales genes markers (gene 1-38, gene 3-53) could be a useful tool in determining the swine-associated impacts of fecal contamination in a watershed.
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http://dx.doi.org/10.1016/j.jes.2019.11.016DOI Listing
April 2020

Acute Methylmercury Exposure and the Hypoxia-Inducible Signaling Pathway under Normoxic Conditions in the Rat Brain and Astrocytes .

Environ Health Perspect 2019 12 18;127(12):127006. Epub 2019 Dec 18.

Department of Preventive Medicine and Public Health Laboratory Sciences, School of Medicine, Jiangsu University, Zhenjiang, China.

Background: As a ubiquitous environmental pollutant, methylmercury (MeHg) induces toxic effects in the nervous system, one of its main targets. However, the exact mechanisms of its neurotoxicity have not been fully elucidated. Hypoxia-inducible (), a transcription factor, plays a crucial role in adaptive and cytoprotective responses in cells and is involved in cell survival, proliferation, apoptosis, inflammation, angiogenesis, glucose metabolism, erythropoiesis, and other physiological activities.

Objectives: The aim of this study was to explore the role of in response to acute MeHg exposure in rat brain and primary cultured astrocytes to improve understanding of the mechanisms of MeHg-induced neurotoxicity and the development of effective neuroprotective strategies.

Methods: Primary rat astrocytes were treated with MeHg () for . Cell proliferation and cytotoxicity were assessed with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl diphenyltetrazolium bromide (MTT) assay and a lactate dehydrogenase (LDH) release assay, respectively. Reactive oxygen species (ROS) levels were analyzed to assess the level of oxidative stress using 2',7'-dichlorofluorescin diacetate (DCFH-DA) fluorescence. , and its downstream proteins, glucose transporter 1 (GLUT-1), erythropoietin (EPO), and vascular endothelial growth factor A (VEGF-A) were analyzed by means of Western blotting. Real-time PCR was used to detect the expression of mRNA. Pretreatment with protein synthesis inhibitor (CHX), proteasome inhibitor (MG132), or proline hydroxylase inhibitor (DHB) were applied to explore the possible mechanisms of inhibition by MeHg. To investigate the role of in MeHg-induced neurotoxicity, cobalt chloride (), 2-methoxyestradiol (2-MeOE2), small interfering RNA (siRNA) transfection and adenovirus overexpression were used. Pretreatment with -acetyl-L-cysteine (NAC) and vitamin E (Trolox) were used to investigate the putative role of oxidative stress in MeHg-induced alterations in levels. The expression of and related downstream proteins was detected in adult rat brain exposed to MeHg () for .

Results: MeHg caused lower cell proliferation and higher cytotoxicity in primary rat astrocytes in a time- and concentration-dependent manner. In comparison with the control cells, exposure to MeHg for significantly inhibited the expression of astrocytic , and the downstream genes GLUT-1, EPO, and VEGF-A (), in the absence of a significant decrease in mRNA levels. When protein synthesis was inhibited by CHX, MeHg promoted the degradation rate of . MG132 and DHB significantly blocked the MeHg-induced decrease in expression (). Overexpression of significantly attenuated the decline in MeHg-induced cell proliferation, whereas the inhibition of significantly increased the decline in cell proliferation (). NAC and Trolox, two established antioxidants, reversed the MeHg-induced decline in protein levels and the decrease in cell proliferation (). MeHg suppressed the expression of and related downstream target proteins in adult rat brain.

Discussion: MeHg induced a significant reduction in protein by activating proline hydroxylase (PHD) and the ubiquitin proteasome system (UPS) in primary rat astrocytes. Additionally, ROS scavenging by antioxidants played a neuroprotective role via increasing expression in response to MeHg toxicity. Moreover, we established that up-regulation of might serve to mitigate the acute toxicity of MeHg in astrocytes, affording a novel therapeutic target for future exploration. https://doi.org/10.1289/EHP5139.
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http://dx.doi.org/10.1289/EHP5139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957278PMC
December 2019

Design, synthesis, and biological study of 4-[(2-nitroimidazole-1-alkyloxyl)aniline]-quinazolines as EGFR inhibitors exerting cytotoxicities both under normoxia and hypoxia.

Drug Des Devel Ther 2019 28;13:3079-3089. Epub 2019 Aug 28.

Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.

Purpose: In order to get novel EGFR inhibitors exerting more potency in tumor hypoxia than in normoxia.

Methods: A series of 4-[(2-nitroimidazole-1-alkyloxyl)aniline]-quinazolines were designed and synthesized, and their in vitro cytotoxicity and EGFR inhibitory activity were evaluated. Molecule docking study was performed for the representative compound.

Results: The structure-activity relationship (SAR) studies revealed that compounds bearing both meta-chloride and para-(2-nitroimidazole-1-alkyloxy) groups on the aniline displayed potent inhibitory activities both in enzymatic and cellular levels. The most promising compound potently inhibited EGFR with an IC value of 0.12 μM. Meanwhile, it manifested more potent cytotoxicity than the positive control lapatinib under tumor normoxia and hypoxia conditions (IC values of 1.59 and 1.09 μM against A549 cells, 2.46 and 1.35 μM against HT-29 cells, respectively). The proposed binding model of in complex with EGFR was displayed by the docking results.

Conclusion: This study provides insights for developing hypoxia-activated kinase inhibitors.
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http://dx.doi.org/10.2147/DDDT.S209481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717862PMC
March 2020

Europium metal-organic framework for selective and sensitive detection of doxycycline based on fluorescence enhancement.

Talanta 2020 Jan 26;207:120297. Epub 2019 Aug 26.

MOE Key Laboratory of Resources and Environmental System Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing, 102206, People's Republic of China; College of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, People's Republic of China; Department of Chemistry, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi Arabia. Electronic address:

Metal-organic framework is an extended coordination compound with repeating coordination entities and voids, which shows potential applications in gas storage, separation, catalysis, and etc. This work reports the synthesis of a new functional metal-organic framework of pyromellitic acid and europium, and its application in fluorescence sensing of doxycycline, a broad-spectrum polyketide antibiotic on the basis of fluorescence turn-on method. The metal-organic framework was initially non-fluorescent, however, with the addition of doxycycline, the system exhibited significant fluorescence enhancement at 526 nm and 617 nm, where both fluorescence intensities showed good linear correlations with the doxycycline concentration, suggesting the possibility for direct two-channel detection without any sensitization materials such as metal particles or surface-modified quantum dots. More interestingly, the unique fluorescence response of the system could discriminate doxycycline from other tetracycline antibiotics with high selectivity, and a limit of detection (LOD) was estimated to be 47 nM. It has been further demonstrated that the material has been successfully applied as sensing materials for selective determination of doxycycline in real samples of fish and urine.
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http://dx.doi.org/10.1016/j.talanta.2019.120297DOI Listing
January 2020

Construction of a high density linkage map and genome dissection of bruchid resistance in zombi pea (Vigna vexillata (L.) A. Rich).

Sci Rep 2019 08 12;9(1):11719. Epub 2019 Aug 12.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Zombi pea (Vigna vexillata) is a legume crop that is resistant to several biotic and abiotic stresses. Callosobruchus maculatus and Callosobruchus chinensis are serious stored-insect pests of legume crops. We constructed a high-density linkage map and performed quantitative trait loci (QTLs) mapping for resistance to these insect species in zombi pea. An F population of 198 individuals from a cross between 'TVNu 240' (resistant) and 'TVNu 1623' (susceptible) varieties was used to construct a linkage map of 6,529 single nucleotide polymorphism markers generated from sequencing amplified fragments of specific loci. The map comprised 11 linkage groups, spanning 1,740.9 cM, with an average of 593.5 markers per linkage group and an average distance of 0.27 cM between markers. High levels of micro-synteny between V. vexillata and cowpea (Vigna unguiculata), mungbean (Vigna radiata), azuki bean (Vigna angularis) and common bean (Phaseolus vulgaris) were found. One major and three minor QTLs for C. chinensis resistance and one major and one minor QTLs for C. maculatus resistance were identified. The major QTLs for resistance to C. chinensis and C. maculatus appeared to be the same locus. The linkage map developed in this study will facilitate the identification of useful genes/QTLs in zombi pea.
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http://dx.doi.org/10.1038/s41598-019-48239-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690978PMC
August 2019

Curcumin protects against methylmercury-induced cytotoxicity in primary rat astrocytes by activating the Nrf2/ARE pathway independently of PKCδ.

Toxicology 2019 09 19;425:152248. Epub 2019 Jul 19.

Department of Preventive Medicine and Public Health Laboratory Sciences, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; Center for Experimental Research, Kunshan Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu 215132, China. Electronic address:

Methylmercury (MeHg) is a ubiquitous environmental toxicant that leads to long-lasting neurological deficits in animals and humans. Curcumin, a polyphenol obtained from the rhizome of turmeric, has well-known antioxidant functions. Here, we evaluated curcumin's efficacy in mitigating MeHg-induced cytotoxicity and further investigated the underlying mechanism of this neuroprotection in primary rat astrocytes. Pretreatment with curcumin (2, 5, 10 and 20 μM for 3, 6, 12 or 24 h) protected against MeHg-induced (5 μM for 6 h) cell death in a time and dose-dependent manner. Curcumin (2, 5, 10 or 20 μM) pretreatment for 12 h significantly ameliorated the MeHg-induced astrocyte injury and oxidative stress, as evidenced by morphological alterations, lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) generation, and glutathione (GSH) and catalase (CAT) levels. Moreover, curcumin pretreatment increased Nrf2 nuclear translocation and downstream enzyme expression, heme oxygenase-1 (HO-1) and NADPH quinone reductase-1 (NQO1). Knockdown of Nrf2 with siRNA attenuated the protective effect of curcumin against MeHg-induced cell death. However, both the pan-protein kinase C (PKC) inhibitor, Ro 31-8220, and the selective PKCδ inhibitor, rottlerin, failed to suppress the curcumin-activated Nrf2/Antioxidant Response Element(ARE) pathway and attenuate the protection exerted by curcumin. Taken together, these findings confirm that curcumin protects against MeHg-induced neurotoxicity by activating the Nrf2/ARE pathway and this protection is independent of PKCδ activation. More studies are needed to understand the mechanisms of curcumin cytoprotection.
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http://dx.doi.org/10.1016/j.tox.2019.152248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710134PMC
September 2019

Susceptibility to the acute toxicity of acrylonitrile in streptozotocin-induced diabetic rats: protective effect of phenethyl isothiocyanate, a phytochemical CYP2E1 inhibitor.

Drug Chem Toxicol 2021 Mar 1;44(2):130-139. Epub 2019 Jul 1.

Department of Preventive Medicine and Public Health Laboratory Sciences, School of Medicine, Jiangsu University, Zhenjiang, PR China.

Diabetes mellitus is a significant global public health issue. The diabetic state not only precipitates chronic disease but also has the potential to change the toxicity of drugs and chemicals. Acrylonitrile (AN) is a potent neurotoxin widely used in industrial products. This study used a streptozotocin (STZ)-induced diabetic rat model to examine the role of cytochrome P450 2E1 (CYP2E1) in acute AN toxicity. The protective effect of phenethyl isothiocyanate (PEITC), a phytochemical inhibitor of CYP2E1, was also investigated. A higher incidence of convulsions and loss of the righting reflex, and decreased rates of survival, as well as elevated CYP2E1 activity, were observed in diabetic rats treated with AN when compared to those in non-diabetic rats, suggesting that diabetes confers susceptibility to the acute toxicity of AN. Pretreatment with PEITC (20-80 mg/kg) followed by AN injection alleviated the acute toxicity of AN in diabetic rats as evidenced by the decreased incidence of convulsions and loss of righting reflex, and increased rates of survival. PEITC pretreatment at 40 and 80 mg/kg decreased hepatic CYP2E1 activity in AN-exposed diabetic rats. PEITC pretreatment (20 mg/kg) increased the glutathione (GSH) content and glutathione S-transferase (GST) activity and further decreased ROS levels in AN-exposed diabetic rats. Collectively, STZ-induced diabetic rats were more sensitive to AN-induced acute toxicity mainly due to CYP2E1 induction, and PEITC pretreatment significantly alleviated the acute toxicity of AN in STZ-induced diabetic rats. PEITC might be considered as a potential effective chemo-preventive agent against AN-induced acute toxicity in individuals with an underlying diabetic condition.
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http://dx.doi.org/10.1080/01480545.2019.1566354DOI Listing
March 2021

Comparing the protective effects of three sulfur compounds against acrylonitrile-induced acute toxicity in CYP2E1-induced rats.

Toxicol Ind Health 2019 May 16;35(5):387-397. Epub 2019 Apr 16.

1 School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.

Cytochrome P450 2E1 (CYP2E1) can be induced by diabetes mellitus, nonalcoholic liver disease, and obesity. This study assessed the protective effects of three sulfur compounds, namely phenethyl isothiocyanate (PEITC), dimethyl trisulfide (DMTS), and sodium thiosulfate (STS), on acrylonitrile (ACN)-induced acute toxicity in rats enriched with CYP2E1. PEITC and DMTS were administered intragastrically (i.g.), whereas STS was injected intraperitoneally (i.p.) at an identical dose of 0.5 mmol/kg for 3 days in acetone-pretreated rats before ACN (90 mg/kg) injection (i.p.). Acetone-treated rats that expressed high levels of CYP2E1 were more susceptible to ACN-induced acute toxicity. The sulfur compounds reduced the rate of convulsions and loss of the righting reflex in acute ACN-exposed CYP2E1-induced rats; PEITC and DMTS also increased the survival rates. PEITC inhibited hepatic CYP2E1 activity and protected hepatic and cerebral cytochrome c oxidase (CcOx) activities in acute ACN-exposed CYP2E1-enriched rats; DMTS protected hepatic CcOx activity. DMTS attenuated ACN-induced oxidative injury by reducing malondialdehyde (MDA) levels and increasing glutathione content in the brain. STS only reduced cerebral MDA levels, whereas PEITC did not exhibit any antioxidant effects. Collectively, PEITC provided superior protective effects against ACN-induced acute toxicity in rats with increased CYP2E1 activity, followed by DMTS; STS provided limited effects. PEITC and DMTS might be considered as promising chemopreventive agents against ACN-induced acute toxicity in vulnerable subpopulations with increased CYP2E1 activity.
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http://dx.doi.org/10.1177/0748233719839847DOI Listing
May 2019

Metal-Organic Framework Enhances Aggregation-Induced Fluorescence of Chlortetracycline and the Application for Detection.

Anal Chem 2019 05 23;91(9):5913-5921. Epub 2019 Apr 23.

MOE Key Laboratory of Resources and Environmental System Optimization, College of Environmental Science and Engineering , North China Electric Power University , Beijing 102206 , China.

The development of an analytical method for selective and sensitive detection of chlortetracycline (CTC), an often overused broad spectrum antibiotic, is important and challenging in environmental and health monitoring. This paper reports a zinc based metal-organic framework of pyromellitic acid (Zn-BTEC), which has been found to greatly enhance the aggregation-induced emission (AIE) of chlortetracycline. The unique emission response of CTC on Zn-BTEC has been extensively examined and applied for the sensitive detection of CTC on the basis of fluorescence intensity of AIE, and a limit of detection (LOD) was estimated to be 28 nM. A rational mechanism has been proposed based on the porous structure of Zn-BTEC, and the CTC molecules would defuse into the rigid MOF structure and assemble or aggregate, leading to fluorescence enhancement of CTC. Interestingly, the Zn-BTEC materials could discriminate CTC from other TC antibiotics with high selectivity. We have further demonstrated that the Zn-BTEC materials are successfully applied for the sensitive and selective determination of CTC in real samples of fish and urine.
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http://dx.doi.org/10.1021/acs.analchem.9b00319DOI Listing
May 2019

Exposure of low-concentration arsenic-initiated inflammation and autophagy in rat lungs.

J Biochem Mol Toxicol 2019 Jul 8;33(7):e22334. Epub 2019 Apr 8.

School of Public Health, Baotou Medical College, Baotou, Inner Mongolia, China.

Chronic arsenic exposure through water intake is a worldwide issue, which has caused many diseases. Lungs are the first target organ of arsenic and lung inflammation, autophagy, and even the onset of tumors can be induced by arsenic exposure. Here, we tested the outcome of low-concentration arsenic exposure in rat lungs. Tissue changes, inflammation, autophagy, and other physiological responses were observed in this study. Results showed that low-concentration exposure of arsenite through water intake could initiate autophagy and inflammation in lungs but high concentration exposure produced a weak autophagy response and accentuated inflammation with the possibility of a chronic inflammation environment emerging followed by tumorigenesis.
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http://dx.doi.org/10.1002/jbt.22334DOI Listing
July 2019

Selective oxidation of glycerol to tartronic acid over Pt/N-doped mesoporous carbon with extra framework magnesium catalysts under base-free conditions.

Chem Commun (Camb) 2019 Feb;55(18):2620-2623

Guangdong Provincial Key Laboratory of Petrochemical Pollution Process and Control, Guangdong University of Petrochemical Technology, Maoming 525000, P. R. China.

N-Doped mesoporous carbons (NMCs) with extra framework magnesium were prepared by a one-pot method and used as supports for Pt catalysts. The surface basicity of NMC improved in the presence of extra framework magnesium (e.g., -Ph-O-Mg), meanwhile, the electron density of Pt was enriched by the electron transfer from graphitic N in NMC to Pt. As a result, the catalytic activity of the supported Pt catalyst was improved to be able to selectively oxidize glycerol (GLY) to tartronic acid (TA) under base-free conditions.
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http://dx.doi.org/10.1039/c8cc10273bDOI Listing
February 2019