Publications by authors named "Su Yang"

530 Publications

Challenges and prospects for a potential allohexaploid Brassica crop.

Theor Appl Genet 2021 Jun 4. Epub 2021 Jun 4.

Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China.

The production of a new allohexaploid Brassica crop (2n = AABBCC) is increasingly attracting international interest: a new allohexaploid crop could benefit from several major advantages over the existing Brassica diploid and allotetraploid species, combining genetic diversity and traits from all six crop species with additional allelic heterosis from the extra genome. Although early attempts to produce allohexaploids showed mixed results, recent technological and conceptual advances have provided promising leads to follow. However, there are still major challenges which exist before this new crop type can be realized: (1) incorporation of sufficient genetic diversity to form a basis for breeding and improvement of this potential crop species; (2) restoration of regular meiosis, as most allohexaploids are genetically unstable after formation; and (3) improvement of agronomic traits to the level of "elite" breeding material in the diploid and allotetraploid crop species. In this review, we outline these major prospects and challenges and propose possible plans to produce a stable, diverse and agronomically viable allohexaploid Brassica crop.
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http://dx.doi.org/10.1007/s00122-021-03845-8DOI Listing
June 2021

Highly selective and robust single-atom catalyst Ru/NC for reductive amination of aldehydes/ketones.

Nat Commun 2021 Jun 2;12(1):3295. Epub 2021 Jun 2.

CAS Key Laboratory of Science and Technology on Applied Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

Single-atom catalysts (SACs) have emerged as a frontier in heterogeneous catalysis due to the well-defined active site structure and the maximized metal atom utilization. Nevertheless, the robustness of SACs remains a critical concern for practical applications. Herein, we report a highly active, selective and robust Ru SAC which was synthesized by pyrolysis of ruthenium acetylacetonate and N/C precursors at 900 °C in N followed by treatment at 800 °C in NH. The resultant Ru-N structure exhibits moderate capability for hydrogen activation even in excess NH, which enables the effective modulation between transimination and hydrogenation activity in the reductive amination of aldehydes/ketones towards primary amines. As a consequence, it shows superior amine productivity, unrivalled resistance against CO and sulfur, and unexpectedly high stability under harsh hydrotreating conditions compared to most SACs and nanocatalysts. This SAC strategy will open an avenue towards the rational design of highly selective and robust catalysts for other demanding transformations.
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http://dx.doi.org/10.1038/s41467-021-23429-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172939PMC
June 2021

Treatment of Heart Failure With Mid-Range Ejection Fraction: A Summary of Current Evidence.

Front Cardiovasc Med 2021 12;8:653336. Epub 2021 May 12.

Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

Heart failure (HF) is a complex syndrome causing heavy burden in public health, and the modern objective assessment of it is based on the left ventricular ejection fraction (LVEF). In 2016, the European Society of Cardiology classified the "gray area" in HF with LVEF of 40-49% as a new HF phenotype (HFmrEF) in an attempt to uncover the specific characteristics and treatment of these patients, which might recover or worsen to HFpEF or HFrEF, respectively, or conversely from these two subtypes. Up to now, many studies have demonstrated that patients with HFmrEF would possibly gain more benefits from some targeted therapies with HFrEF than those with HFpEF. This review summarizes what is known about the findings in the treatment of HFmrEF and discusses what should be done to better define the peculiar HF phenotype in the future.
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http://dx.doi.org/10.3389/fcvm.2021.653336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149608PMC
May 2021

Robust Electrodes for Flexible Energy Storage Devices Based on Bimetallic Encapsulated Core-Multishell Structures.

Adv Sci (Weinh) 2021 May 29:e2100911. Epub 2021 May 29.

Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, M5S 3H6, Canada.

Developing flexible electrodes with high active materials loading and excellent mechanical stability is of importance to flexible electronics, yet remains challenging. Herein, robust flexible electrodes with an encapsulated core-multishell structure are developed via a spraying-hydrothermal process. The multilayer electrode possesses an architecture of substrate/reduced graphene oxide (rGO)/bimetallic complex/rGO/bimetallic complex/rGO from the inside to the outside, where the cellulosic fibers serve as the substrate, namely, the core; and the multiple layers of rGO and bimetallic complex, are used as active materials, namely, the shells. The inner two rGO interlayers function as the cement that chemically bind to two adjacent layers, while the two outer rGO layers encapsulate the inside structure effectively protecting the electrode from materials detachment or electrolyte corrosion. The electrodes with a unique core-multishell structure exhibit excellent cycle stability and exceptional temperature tolerance (-25 to 40 °C) for lithium and sodium storage. A combination of experimental and theoretical investigations are carried out to gain insights into the synergetic effects of cobalt-molybdenum-sulfide (CMS) materials (the bimetallic complex), which will provide guidance for future exploration of bimetallic sulfides. This strategy is further demonstrated in other substrates, showing general applicability and great potential in the development of flexible energy storage devices.
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http://dx.doi.org/10.1002/advs.202100911DOI Listing
May 2021

IP-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion.

Nat Commun 2021 04 28;12(1):2461. Epub 2021 Apr 28.

School of Life Sciences, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China.

COP1 and COP9 signalosome (CSN) are the substrate receptor and deneddylase of CRL4 E3 ligase, respectively. How they functionally interact remains unclear. Here, we uncover COP1-CSN antagonism during glucose-induced insulin secretion. Heterozygous Csn2 mice with partially disrupted binding of IP, a CSN cofactor, display congenital hyperinsulinism and insulin resistance. This is due to increased Cul4 neddylation, CRL4 E3 assembly, and ubiquitylation of ETV5, an obesity-associated transcriptional suppressor of insulin secretion. Hyperglycemia reciprocally regulates CRL4-CSN versus CRL4 assembly to promote ETV5 degradation. Excessive ETV5 degradation is a hallmark of Csn2, high-fat diet-treated, and ob/ob mice. The CRL neddylation inhibitor Pevonedistat/MLN4924 stabilizes ETV5 and remediates the hyperinsulinemia and obesity/diabetes phenotypes of these mice. These observations were extended to human islets and EndoC-βH1 cells. Thus, a CRL4-ETV5 proteolytic checkpoint licensing GSIS is safeguarded by IP-assisted CSN-COP1 competition. Deregulation of the IP-CSN-CRL4-ETV5 axis underlies hyperinsulinemia and can be intervened to reduce obesity and diabetic risk.
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http://dx.doi.org/10.1038/s41467-021-22941-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080631PMC
April 2021

SynNotch CAR circuits enhance solid tumor recognition and promote persistent antitumor activity in mouse models.

Sci Transl Med 2021 Apr;13(591)

Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.

The first clinically approved engineered chimeric antigen receptor (CAR) T cell therapies are remarkably effective in a subset of hematological malignancies with few therapeutic options. Although these clinical successes have been exciting, CAR T cells have hit roadblocks in solid tumors that include the lack of highly tumor-specific antigens to target, opening up the possibility of life-threatening "on-target/off-tumor" toxicities, and problems with T cell entry into solid tumor and persistent activity in suppressive tumor microenvironments. Here, we improve the specificity and persistent antitumor activity of therapeutic T cells with synthetic Notch (synNotch) CAR circuits. We identify alkaline phosphatase placental-like 2 (ALPPL2) as a tumor-specific antigen expressed in a spectrum of solid tumors, including mesothelioma and ovarian cancer. ALPPL2 can act as a sole target for CAR therapy or be combined with tumor-associated antigens such as melanoma cell adhesion molecule (MCAM), mesothelin, or human epidermal growth factor receptor 2 (HER2) in synNotch CAR combinatorial antigen circuits. SynNotch CAR T cells display superior control of tumor burden when compared to T cells constitutively expressing a CAR targeting the same antigens in mouse models of human mesothelioma and ovarian cancer. This was achieved by preventing CAR-mediated tonic signaling through synNotch-controlled expression, allowing T cells to maintain a long-lived memory and non-exhausted phenotype. Collectively, we establish ALPPL2 as a clinically viable cell therapy target for multiple solid tumors and demonstrate the multifaceted therapeutic benefits of synNotch CAR T cells.
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http://dx.doi.org/10.1126/scitranslmed.abd8836DOI Listing
April 2021

Characteristics of anammox granular sludge using color differentiation, and nitrogen removal performance of its immobilized fillers based on microbial succession.

Bioresour Technol 2021 Aug 18;333:125188. Epub 2021 Apr 18.

Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, College of Architectural Engineering, Beijing University of Technology, Beijing 100124, China.

The characteristics of anammox granular sludge (AnGS) based on color differentiation, and the regulation mechanism of immobilized fillers in the system were investigated. The results showed that biomass content, EPS and activity of red AnGS (R1) were higher than those of brown AnGS (R2). Moreover, R1 showed nitrification, while R2 showed denitrification. Filamentous bacteria constituted the granule skeleton of R1, while R2 mainly constituted inorganic nucleation and granulation. Additionally, immobilization improved the contribution rate of Anammox, and involved different regulatory mechanisms. High-throughput sequencing analysis showed that R1 encapsulation biomass eliminated miscellaneous bacteria and established specific flora, while mixed encapsulated biomass of R1 and R2 re-formed a functional bacterial network, which strengthened interspecies cooperation. The R2 encapsulated biomass and AnAOB copy numbers were inferior and the interspecific cooperation was weak, resulting in an unsatisfactory nitrogen removal performance. These results can strengthen the understanding and optimization of AnGS and its immobilization system.
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http://dx.doi.org/10.1016/j.biortech.2021.125188DOI Listing
August 2021

Construction of Competitive Endogenous RNA Network and Verification of 3-Key LncRNA Signature Associated With Distant Metastasis and Poor Prognosis in Patients With Clear Cell Renal Cell Carcinoma.

Front Oncol 2021 24;11:640150. Epub 2021 Mar 24.

Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Clear cell renal cell carcinoma (ccRCC) is a common malignancy with high distant metastasis rate. Long non-coding RNAs (LncRNAs) are reported to be upregulated or downregulated in multiple cancers and play a crucial role in the metastasis of tumors or prognosis. Therefore, the purpose of our study is to construct a prognostic signature for ccRCC based on distant metastasis-related lncRNAs and explore the involved potential competitive endogenous RNA (ceRNA) network. The differentially expressed genes (DEGs) screened from the database of the cancer genome atlas (TCGA) were used to construct a co-expression network and identify the distant metastasis-related module by weighted gene co-expression network analysis (WGCNA). Key genes with metastatic and prognostic significance were identified through rigorous screening, including survival analysis, correlation analysis, and expression analyses in stage, grade, and distant metastasis, and were verified in the data set of gene expression omnibus (GEO) and the database from gene expression profiling interactive analysis (GEPIA). The potential upstream miRNAs and lncRNAs were predicted five online databases and LncBase. Here, we constructed a ceRNA network of key genes that are significantly associated with the distant metastasis and prognosis of patients with ccRCC. The distant metastasis-related lncRNAs were used to construct a risk score model through the univariate, least absolute shrinkage selection operator (LASSO), and multivariate Cox regression analyses, and the patients were divided into high- and low-risk groups according to the median of the risk score. The Kaplan-Meier survival analysis demonstrated that mortality was significantly higher in the high-risk group than in the low-risk group. Considering the other clinical phenotype, the Cox regression analyses indicated that the lncRNAs model could function as an independent prognostic factor. Quantitative real-time (qRT)-PCR in the tissues and cells of ccRCC verified the high-expression level of three lncRNAs. Gene set enrichment analysis (GSEA) revealed that the lncRNA prognostic signature was mainly enriched in autophagy- and immune-related pathways, indicating that the autophagy and immune functions may play an important role in the distant metastasis of ccRCC. In summary, the constructed distant metastasis-related lncRNA signature could independently predict prognosis in patients with ccRCC, and the related ceRNA network provided a new sight on the potential mechanism of distant metastasis and a promising therapeutic target for ccRCC.
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http://dx.doi.org/10.3389/fonc.2021.640150DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044754PMC
March 2021

Increase in arsenic methylation and volatilization during manure composting with biochar amendment in an aeration bioreactor.

J Hazard Mater 2021 06 13;411:125123. Epub 2021 Jan 13.

Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou 310058, China.

Biochar is widely used as an amendment to optimize the composting process. In this study, we firstly investigated the effects of biochar amendment on methylation and volatilization of arsenic (As), and the microbial communities during manure composting. Biochar amendment was found to increase the concentrations of monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) during mesophilic (days 0-10) and early thermophilic (days 11-15) phases, and promote As volatilization during the maturing phase (days 60-80) of composting. In addition, the abundances of As(V) reductase (arsC) and As(III) S-adenosyl-L-methionine methyltransferase (arsM) genes were higher in the biochar treatment than that in the control. Moreover, biochar amendment influenced the microbial communities by promoting As methylation and volatilization via Ensifer and Sphingobium carrying arsC genes, and Rhodopseudomonas and Pseudomonas carrying arsM genes. This study emphasized the considerable role of biochar on methylation and volatilization of As during manure composting and provided an overall characterization of the community compositions of arsC and arsM genes during manure composting. It will broaden our insights in As biogeochemical cycle during manure composting with biochar amendment, which will facilitate the regulation of As during manure composting and its application in agricultural soil.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125123DOI Listing
June 2021

Interleukin-17D regulates group 3 innate lymphoid cell function through its receptor CD93.

Immunity 2021 Apr;54(4):673-686.e4

Institute for Immunology and School of Medicine, Tsinghua University, Beijing 100084, China; Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai 200127, China. Electronic address:

The interleukin (IL)-17 family, consisting of six members, promotes host defense but can in some context promote the development of autoimmune disease. Here, we examined the role of IL-17D, a poorly understood member in the IL-17 family. IL-17D was expressed primarily by colonic epithelial cells. Il17d mice were more susceptible to acute colitis, bacterial infection and experimentally induced colon cancer than their wildtype counterparts. Il17d deficiency impaired IL-22 production by group 3 innate lymphoid cells (ILC3s) and reduced expression of IL-22-dependent antimicrobial peptides, RegIIIβ and RegIIIγ, in colon tissue at steady state and in colitis; this was associated with changes in microbial composition and dysbiosis. Protein purification studies revealed that IL-17D bound not canonical IL-17 receptors, but rather CD93, a glycoprotein expressed on mature ILC3s. Mice lacking Cd93 in ILC3s exhibited impaired IL-22 production and aggravated colonic inflammation in experimental colitis. Thus, an IL-17D-CD93 axis regulates ILC3 function to preserve intestinal homeostasis.
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http://dx.doi.org/10.1016/j.immuni.2021.03.018DOI Listing
April 2021

Stereo- and regiodefined DNA-encoded chemical libraries enable efficient tumour-targeting applications.

Nat Chem 2021 Jun 8;13(6):540-548. Epub 2021 Apr 8.

Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETH Zurich), Zurich, Switzerland.

The encoding of chemical compounds with amplifiable DNA tags facilitates the discovery of small-molecule ligands for proteins. To investigate the impact of stereo- and regiochemistry on ligand discovery, we synthesized a DNA-encoded library of 670,752 derivatives based on 2-azido-3-iodophenylpropionic acids. The library was selected against multiple proteins and yielded specific ligands. The selection fingerprints obtained for a set of protein targets of pharmaceutical relevance clearly showed the preferential enrichment of ortho-, meta- or para-regioisomers, which was experimentally verified by affinity measurements in the absence of DNA. The discovered ligands included novel selective enzyme inhibitors and binders to tumour-associated antigens, which enabled conditional chimeric antigen receptor T-cell activation and tumour targeting.
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http://dx.doi.org/10.1038/s41557-021-00660-yDOI Listing
June 2021

Highly active and stable Ir nanoclusters derived from Ir/MgAlO single-atom catalysts.

J Chem Phys 2021 Apr;154(13):131105

CAS Key Laboratory of Science and Technology on Applied Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Single-atom catalysts (SACs) prepared by the atom trapping method often possess high stability yet have limited advantages regarding catalytic performance due to the strong metal-support interaction. Using these SACs as seeds to develop supported nanoclusters or nanoparticles has, however, been proven to be effective in improving the catalysts' intrinsic activity. Herein, we have prepared extremely stable Ir SACs supported by MgAlO via atomic trapping and used them as seeds to fabricate highly active and stable Ir nanocluster catalysts by high-temperature reduction. The activity toward NO decomposition increased by more than ten times compared with that of the parent Ir SACs. This study provides a new avenue to design and develop highly active and stable catalysts for industrial use.
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http://dx.doi.org/10.1063/5.0048565DOI Listing
April 2021

Highly efficient Co single-atom catalyst for epoxidation of plant oils.

J Chem Phys 2021 Apr;154(13):131103

CAS Key Laboratory of Science and Technology on Applied Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Epoxidation of bio-derived plant oils is a sustainable route to manufacturing plasticizers, additives in lubricants, and other chemicals. The traditional synthetic approaches suffer from the employment of corrosive mineral acid or expensive peroxides (e.g., HO). In this work, we report the epoxidation of plant oils using O as the terminal oxidant catalyzed by Co-N-C/SiO single-atom catalyst. The single-atom dispersion of cobalt is confirmed by high-angle annular dark field-STEM and x-ray absorption fine structure techniques. In the epoxidation of methyl oleate under mild reaction conditions (35 °C, 0.1 MPa O), 99% selectivity to the desired product is achieved at full conversion. Even for crude oils, Co-N-C/SiO is also effective and good yields of the corresponding epoxides are obtained. In addition, the catalyst is easily recovered and can be reused five times without obvious decay in catalytic activity/selectivity. A superoxide radical involved reaction mechanism is proposed on the basis of kinetic study and EPR experiment.
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http://dx.doi.org/10.1063/5.0046166DOI Listing
April 2021

Ionic Liquid-Induced Ostwald Ripening Effect for Efficient and Stable Tin-Based Perovskite Solar Cells.

ACS Appl Mater Interfaces 2021 Apr 24;13(13):15420-15428. Epub 2021 Mar 24.

College of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.

Tin-based perovskite solar cells (PVSCs) are regarded as the most promising alternative among lead-free PVSCs. However, the rapid crystallization for tin-based perovskite tends to cause inferior film morphology and abundant defect states, which make poor photovoltaic performance. Here, 1-butyl-3-methylimidazolium bromide (BMIBr) ionic liquids (ILs) with strong polarity and a low melting point are first employed to produce the Ostwald ripening effect and obtain high-quality tin-based perovskite films with a large grain size. Meanwhile, the non-radiative recombination ascribed from defect states can also be effectively reduced for BMIBr-treated perovskite films. Consequently, a photoelectric conversion efficiency (PCE) of 10.09% for inverted tin-based PVSCs is attained by the Ostwald ripening effect. Moreover, the unencapsulated devices with BMIBr retain near 85% of the original PCE in a N glovebox beyond 1200 h and about 40% of the original PCE after exposure to air for 48 h.
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http://dx.doi.org/10.1021/acsami.1c01408DOI Listing
April 2021

Single-Cell Transcriptome Analysis Decipher New Potential Regulation Mechanism of ACE2 and NPs Signaling Among Heart Failure Patients Infected With SARS-CoV-2.

Front Cardiovasc Med 2021 23;8:628885. Epub 2021 Feb 23.

Kelly Government Solutions, Rockville, MD, United States.

COVID-19 patients with comorbidities such as hypertension or heart failure (HF) are associated with poor clinical outcomes. The cellular distribution of Angiotensin-converting enzyme 2 (ACE2), the critical enzyme for SARS-CoV-2 infection, in the human heart is unknown. We explore the underlying mechanism that leads to increased susceptibility to SARS-CoV-2 in patients with cardiovascular diseases and patients of cardiac dysfunction have increased risk of multi-organ injury compared with patients of normal cardiac function. We analyzed single-cell RNA sequencing (scRNA-seq) data in both normal and failing hearts. The results demonstrated that ACE2 is present in cardiomyocytes (CMs) and non-CMs, while the number of ACE2-postive (ACE2+) CMs and ACE2 gene expression in these CMs are significantly increased in the failing hearts. Interestingly, both brain natriuretic peptides (BNP) and atrial natriuretic peptide (ANP) are significantly up-regulated in the ACE2+ CMs, which is consistent with other studies that ACE2, ANP, and BNP increased in HF patients. We found that genes related to virus entry, virus replication and suppression of interferon-gamma signaling are all up-regulated in failing CMs, and the increase was significantly higher in ACE2+ CMs, suggesting that these CMs may be more vulnerable to virus infection. As the level of expression of both ACE2 and BNP in CMs were up-regulated, we further performed retrospective analysis of the plasma BNP levels and clinical outcomes of 91 COVID-19 patients from a single-center. Patients with higher plasma BNP were associated with significantly higher mortality and expression levels of inflammatory and infective markers. In the failing heart, the upregulation of ACE2 and virus infection associated genes could potentially facilitate SARS-CoV-2 virus entry and replication in these vulnerable cardiomyocyte subsets. COVID-19 patients with higher plasma BNP levels had poorer clinical outcomes. These observations may allude to a potential regulatory association between ACE2 and BNP in mediating myocarditis associated with COVID-19.
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http://dx.doi.org/10.3389/fcvm.2021.628885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952310PMC
February 2021

Alzheimer's disease detection using depthwise separable convolutional neural networks.

Comput Methods Programs Biomed 2021 May 2;203:106032. Epub 2021 Mar 2.

College of Foreign Languages, University of Shanghai for Science and Technology, Shanghai, China.

To diagnose Alzheimer's disease (AD), neuroimaging methods such as magnetic resonance imaging have been employed. Recent progress in computer vision with deep learning (DL) has further inspired research focused on machine learning algorithms. However, a few limitations of these algorithms, such as the requirement for large number of training images and the necessity for powerful computers, still hinder the extensive usage of AD diagnosis based on machine learning. In addition, large number of training parameters and heavy computation make the DL systems difficult in integrating with mobile embedded devices, for example the mobile phones. For AD detection using DL, most of the current research solely focused on improving the classification performance, while few studies have been done to obtain a more compact model with less complexity and relatively high recognition accuracy. In order to solve this problem and improve the efficiency of the DL algorithm, a deep separable convolutional neural network model is proposed for AD classification in this paper. The depthwise separable convolution (DSC) is used in this work to replace the conventional convolution. Compared to the traditional neural networks, the parameters and computing cost of the proposed neural network are found greatly reduced. The parameters and computational costs of the proposed neural network are found to be significantly reduced compared with conventional neural networks. With its low power consumption, the proposed model is particularly suitable for embedding mobile devices. Experimental findings show that the DSC algorithm, based on the OASIS magnetic resonance imaging dataset, is very successful for AD detection. Moreover, transfer learning is employed in this work to improve model performance. Two trained models with complex networks, namely AlexNet and GoogLeNet, are used for transfer learning, with average classification rates of 91.40%, 93.02% and a less power consumption.
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http://dx.doi.org/10.1016/j.cmpb.2021.106032DOI Listing
May 2021

Fiber Optic Sensing Technologies for Battery Management Systems and Energy Storage Applications.

Sensors (Basel) 2021 Feb 17;21(4). Epub 2021 Feb 17.

Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA 15260, USA.

Applications of fiber optic sensors to battery monitoring have been increasing due to the growing need of enhanced battery management systems with accurate state estimations. The goal of this review is to discuss the advancements enabling the practical implementation of battery internal parameter measurements including local temperature, strain, pressure, and refractive index for general operation, as well as the external measurements such as temperature gradients and vent gas sensing for thermal runaway imminent detection. A reasonable matching is discussed between fiber optic sensors of different range capabilities with battery systems of three levels of scales, namely electric vehicle and heavy-duty electric truck battery packs, and grid-scale battery systems. The advantages of fiber optic sensors over electrical sensors are discussed, while electrochemical stability issues of fiber-implanted batteries are critically assessed. This review also includes the estimated sensing system costs for typical fiber optic sensors and identifies the high interrogation cost as one of the limitations in their practical deployment into batteries. Finally, future perspectives are considered in the implementation of fiber optics into high-value battery applications such as grid-scale energy storage fault detection and prediction systems.
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http://dx.doi.org/10.3390/s21041397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923102PMC
February 2021

An Early Stage Researcher's Primer on Systems Medicine Terminology.

Netw Syst Med 2021 Feb 25;4(1):2-50. Epub 2021 Feb 25.

Centre for Molecular Medicine Norway (NCMM), Forskningparken, Oslo, Norway.

Systems Medicine is a novel approach to medicine, that is, an interdisciplinary field that considers the human body as a system, composed of multiple parts and of complex relationships at multiple levels, and further integrated into an environment. Exploring Systems Medicine implies understanding and combining concepts coming from diametral different fields, including medicine, biology, statistics, modeling and simulation, and data science. Such heterogeneity leads to semantic issues, which may slow down implementation and fruitful interaction between these highly diverse fields. In this review, we collect and explain more than100 terms related to Systems Medicine. These include both modeling and data science terms and basic systems medicine terms, along with some synthetic definitions, examples of applications, and lists of relevant references. This glossary aims at being a first aid kit for the Systems Medicine researcher facing an unfamiliar term, where he/she can get a first understanding of them, and, more importantly, examples and references for digging into the topic.
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http://dx.doi.org/10.1089/nsm.2020.0003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919422PMC
February 2021

TRPM2-AS Promotes Bladder Cancer by Targeting miR-22-3p and Regulating GINS2 mRNA Expression.

Onco Targets Ther 2021 23;14:1219-1237. Epub 2021 Feb 23.

Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450000, People's Republic of China.

Background: Bladder cancer (BLCA) refers to the malignancy growth that spreads from the bladder linings to the bladder muscles. However, the impact of miR-22-3p and lncRNA TRPM2-AS on this tumor has generated divergent views in the literature. This research aimed to study the effects of lncRNA TRPM2-AS on BLCA and its interaction with miR-22-3p and GINS2 mRNA.

Methods: qRT-PCR was employed to measure the expression of TRPM2-AS, miR-22-3p and GINS2 mRNA in bladder tissues and cells. The subcellular localization of TRPM2-AS in T24 and 5637 cell lines was identified using a cell fractionation system. Luciferase assay, RIP assay and RNA pull-down assay were later performed to validate the direct binding relationship between TRPM2-AS, miR-22-3p and GINS2 mRNA. Several experiments were conducted to determine the viability, proliferation, colony formation and apoptosis of the cell lines.

Results: Findings indicated that TRPM2-AS was significantly upregulated in BLCA tissues and cell lines. Apart from that, it was observed that TRPM2-AS knockdown significantly inhibited the viability, proliferation and colony formation of BCLA cells, but it promoted the apoptosis of the BCLA cells. A significant downstream target of TRPM2-AS, miR-22-3p was found to show a lower expression level in BLCA tissues and cell lines. However, the inhibition of miR-22-3p considerably enhanced BLCA cell phenotypes. As well as discovering that GINS2 mRNA was a downstream target of miR-22-3p and was significantly upregulated in BLCA, experimental results also indicated that the knockdown of GINS2 suppressed BLCA cell phenotypes.

Conclusion: This research confirmed that TRPM2-AS could promote BCLA by binding to miR-22-3p to increase GINS2 expression. This novel interactome in BLCA cell lines might provide more insights into BLCA therapy.
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http://dx.doi.org/10.2147/OTT.S282151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914110PMC
February 2021

The Top 100 Most Cited Articles on Anterior Cruciate Ligament Reconstruction: A Bibliometric Analysis.

Orthop J Sports Med 2021 Feb 8;9(2):2325967120976372. Epub 2021 Feb 8.

Department of Orthopaedic Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.

Background: The concept of anterior cruciate ligament (ACL) reconstruction (ACLR) has become widely accepted, gaining increased attention in recent years and resulting in many research achievements in this field.

Purpose: The aim of this study was to determine which original articles on ACLR have been most influential in this field by identifying and analyzing the characteristics of the 100 most cited articles.

Study Design: Cross-sectional study.

Methods: Articles on ACLR were identified via the Thomson ISI Web of Science database on November 30, 2019. The 100 most cited articles were identified based on inclusion and exclusion criteria. The data extracted from each article for the subsequent analysis included title, date of publication, total citations, average citations per year (ACY), journal name, first author, institutions, themes, level of evidence, and keywords.

Results: The total number of citations was 29,629. The date of publication ranged from 1975 to 2015. A majority of the articles originated from the United States (58%) and were published in the 1990s (32%) and 2000s (48%). The mean ACY was 18.43 ± 9.51. Of the selected articles, nearly one-half were published in the (42%). The most prolific co-author and first author were Freddie H. Fu (n = 13) and K. Donald Shelbourne (n = 5), respectively. The most productive institution was the University of Pittsburgh (14%). Material comparison (19%) and technique comparison (16%) were the 2 most popular themes. More than one-quarter of articles were level 4 evidence (37%). Moreover, the keywords , , , , , and showed the highest degree of centrality.

Conclusion: By analyzing the characteristics of articles, this study demonstrated that ACLR is a growing and popular area of research, with the focus of research varying through timeline trends. Studies on anatomic reconstruction and biomechanics might be areas of future trends.
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http://dx.doi.org/10.1177/2325967120976372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876772PMC
February 2021

Epigallocatechin-3-gallate inhibits proliferation and triggers apoptosis in colon cancer via the hedgehog/phosphoinositide 3-kinase pathways.

Authors:
Feng Ding Su Yang

Can J Physiol Pharmacol 2021 Feb 22. Epub 2021 Feb 22.

First Affiliated Hospital of Jinzhou Medical University, 457651, No.40.Section 3, Songpo Road, Jinzhou, China, 121002;

The present study evaluated whether EGCG effectively attenuates tumor growth in colon cancer cells and in the xenografts of nude mice and to investigated the underlying mechanisms by focusing on the Shh and PI3K pathways. Three kinds of colon cancer cells and BALB/c nude mice were used to evaluate the antiproliferative effect of EGCG.The results showed that EGCG exhibited an antiproliferative effect against colon cancer cells in a dose-dependent manner with low toxicity against normal colon epithelial cells. Administration of EGCG caused significant apoptosis and inhibited the migration and invasion of colon cancer cells. The toxic effect of EGCG was accompanied by downregulation of the Shh and PI3K/Akt pathways. In addition, EGCG reduced tumor weight without affecting the body weight of nude mice and inhibited the activation of the Shh and PI3K/Akt pathways in tumor tissue. Purmorphamine (Shh agonist) or IGF-1 (PI3K agonist) partly abolished the effect of EGCG on cell proliferation, migration and apoptosis. Cyclopamine (Shh inhibitor) and LY294002 (PI3K inhibitor) showed the similar toxic effects as EGCG on colon cancer cells. In conclusion, EGCG inhibited colon tumor growth via downregulation of the Shh and PI3K pathways and may be a potential chemotherapeutic agent against colon cancer.
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http://dx.doi.org/10.1139/cjpp-2020-0588DOI Listing
February 2021

High probability of yield gain through conservation agriculture in dry regions for major staple crops.

Sci Rep 2021 Feb 8;11(1):3344. Epub 2021 Feb 8.

UMR Agronomie, INRAE AgroParisTech, Université Paris-Saclay, 78850, Thiverval-Grignon, France.

Conservation agriculture (CA) has been promoted to mitigate climate change, reduce soil erosion, and provide a variety of ecosystem services. Yet, its impacts on crop yields remains controversial. To gain further insight, we mapped the probability of yield gain when switching from conventional tillage systems (CT) to CA worldwide. Relative yield changes were estimated with machine learning algorithms trained by 4403 paired yield observations on 8 crop species extracted from 413 publications. CA has better productive performance than no-till system (NT), and it stands a more than 50% chance to outperform CT in dryer regions of the world, especially with proper agricultural management practices. Residue retention has the largest positive impact on CA productivity comparing to other management practices. The variations in the productivity of CA and NT across geographical and climatical regions were illustrated on global maps. CA appears as a sustainable agricultural practice if targeted at specific climatic regions and crop species.
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http://dx.doi.org/10.1038/s41598-021-82375-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870656PMC
February 2021

Harvesting More Energetic Photoexcited Electrons from Closely Packed Gold Nanoparticles.

J Am Soc Mass Spectrom 2021 Mar 8;32(3):815-824. Epub 2021 Feb 8.

Department of Chemistry and State Key Laboratory of Synthetic Chemistry, The University of Hong Kong, Hong Kong S.A.R., P. R. China.

The characterization of photoexcited electrons on the surface of nanomaterial remains challenging. Herein, laser excitation mass spectrometry combined with a chemical thermometer and electron acceptor has been developed to characterize the energetics and population density of photoexcited electrons transferred from gold nanoparticles (AuNPs). In contrast to laser fluence and bias voltage, the hot spots of closely packed AuNPs play a more significant role in enhancing the average energetics of photoexcited electrons, which can be harvested effectively by the electron acceptor. By harvesting more energetic photoexcited electrons for the desorption and ionization process, it is anticipated that the sensitive detection of biomarkers can be achieved, which is beneficial to metabolomic studies and early disease diagnosis.
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http://dx.doi.org/10.1021/jasms.0c00480DOI Listing
March 2021

The Top 100 Most-Cited Articles on Arthroscopy: Most Popular Topic Is Rotator Cuff Rather Than Cartilage in the Last 5 Years.

Arthroscopy 2021 Jun 2;37(6):1779-1797.e1. Epub 2021 Feb 2.

Orthopaedic Department, The Second Xiangya Hospital of Central South University, Changsha, Hunan Province, China. Electronic address:

Purpose: To highlight the characteristics of the 100 most-cited articles on arthroscopy and provide the variation trend of citation rate among the top 25 articles in the past 9 years. We further analyzed the topics of interest in the past or currently.

Methods: The Thomson ISI Web of Science database was used to identify arthroscopy-related articles that were published from 1950 to March 31, 2020. The 100 most-cited articles were selected for further analysis. In addition, author key words of the articles that published in the recent 5 years were further analyzed.

Results: Mean of citations was 433.59 ± 400.73. The publication year ranged from 1980 to 2013. Most articles were focused on cartilage lesions and treatments (26%). A large proportion of articles were published in the 2000s (61%). Arthroscopy-the Journal of Arthroscopic and Related Surgery (23%) was the most popular journal. One half of the articles originated from the United States. The most prolific institution and first author were the Steadman Philippon Research Institute (5%) and Marc J. Philippon (4%), respectively. Most of the articles were Level IV evidence (33%). The citation rate increased by 131% from the previous top 25 articles published in 2011. A growth trend can be seen in the citation density over time. "Shoulder" (occurrences = 535) was the most used joint key word and "rotator cuff" (342) was the most used key word of research objective in the last 5 years, whereas "cartilage" only occurred 262 times.

Conclusions: Based on bibliometric analysis of the 100 most-cited articles on arthroscopy combined with network analysis of the whole articles that published in the recent 5 years, the topic of most interest in the recent 5 years was rotator cuff rather than cartilage. The number of citations among the 25 most-cited articles is growing rapidly and has at least doubled in size on average in the past 9 years. Citation density among the 100 most-cited articles on arthroscopy has seen a growth trend.

Clinical Relevance: This article clarifies the characteristics of the 100 most-cited papers and provides guidance on the topics of interest in the past or currently as a roadmap for future research on arthroscopy.
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http://dx.doi.org/10.1016/j.arthro.2021.01.039DOI Listing
June 2021

High-throughput phenotypic screen and transcriptional analysis identify new compounds and targets for macrophage reprogramming.

Nat Commun 2021 02 3;12(1):773. Epub 2021 Feb 3.

Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.

Macrophages are plastic and, in response to different local stimuli, can polarize toward multi-dimensional spectrum of phenotypes, including the pro-inflammatory M1-like and the anti-inflammatory M2-like states. Using a high-throughput phenotypic screen in a library of ~4000 FDA-approved drugs, bioactive compounds and natural products, we find ~300 compounds that potently activate primary human macrophages toward either M1-like or M2-like state, of which ~30 are capable of reprogramming M1-like macrophages toward M2-like state and another ~20 for the reverse repolarization. Transcriptional analyses of macrophages treated with 34 non-redundant compounds identify both shared and unique targets and pathways through which the tested compounds modulate macrophage activation. One M1-activating compound, thiostrepton, is able to reprogram tumor-associated macrophages toward M1-like state in mice, and exhibit potent anti-tumor activity. Our compound-screening results thus help to provide a valuable resource not only for studying the macrophage biology but also for developing therapeutics through modulating macrophage activation.
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http://dx.doi.org/10.1038/s41467-021-21066-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858590PMC
February 2021

Circular RNA hsa_circ_0004277 Stimulates Malignant Phenotype of Hepatocellular Carcinoma and Epithelial-Mesenchymal Transition of Peripheral Cells.

Front Cell Dev Biol 2020 12;8:585565. Epub 2021 Jan 12.

Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an, China.

Accumulating evidence shows that exosomal circRNAs reflect the physiological status of donor cells, and various cell reactions are induced after exosomal circRNAs are captured by recipient cells. In this study, qRT-PCR was performed to detect circ-0004277 expression in hepatocellular carcinoma (HCC) cell lines, tissues, and plasma exosomes. The effects of circ-0004277 on the proliferation and migration of HCC cells were assessed by cell counting, 5-ethynyl-2'-deoxyuridine assays, Transwell migration assays, and tumor formation in nude mice. We found that circ-0004277 was significantly upregulated in HCC cells, tissues, and plasma exosomes compared to that in normal controls. Overexpression of circ-0004277 enhanced the proliferation, migration, and epithelial-mesenchymal transition (EMT) of HCC cells and . Furthermore, exosomes from HCC cells enhanced circ-0004277 expression in surrounding normal cells and stimulated EMT progression. ZO-1, a tight junction adapter protein, was downregulated in HCC tissues. In conclusion, our findings suggest that circ-0004277 promotes the malignant phenotype of HCC cells via inhibition of and promotion of EMT progression. In addition, exosomal circ-0004277 from HCC cells stimulates EMT of peripheral cells through cellular communication to further promote the invasion of HCC into normal surrounding tissues.
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http://dx.doi.org/10.3389/fcell.2020.585565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835424PMC
January 2021

A global dataset for crop production under conventional tillage and no tillage systems.

Sci Data 2021 01 28;8(1):33. Epub 2021 Jan 28.

UMR Agronomie, INRAE AgroParisTech, Université Paris-Saclay, 78850, Thiverval-Grignon, France.

No tillage (NT) is often presented as a means to grow crops with positive environmental externalities, such as enhanced carbon sequestration, improved soil quality, reduced soil erosion, and increased biodiversity. However, whether NT systems are as productive as those relying on conventional tillage (CT) is a controversial issue, fraught by a high variability over time and space. Here, we expand existing datasets to include the results of the most recent field experiments, and we produce a global dataset comparing the crop yields obtained under CT and NT systems. In addition to crop yield, our dataset also reports information on crop growing season, management practices, soil characteristics and key climate parameters throughout the experimental year. The final dataset contains 4403 paired yield observations between 1980 and 2017 for eight major staple crops in 50 countries. This dataset can help to gain insight into the main drivers explaining the variability of the productivity of NT and the consequence of its adoption on crop yields.
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http://dx.doi.org/10.1038/s41597-021-00817-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844240PMC
January 2021