Publications by authors named "Su Jiang"

84 Publications

Deductive Reinforcement Learning for Visual Autonomous Urban Driving Navigation.

IEEE Trans Neural Netw Learn Syst 2021 Sep 14;PP. Epub 2021 Sep 14.

Existing deep reinforcement learning (RL) are devoted to research applications on video games, e.g., The Open Racing Car Simulator (TORCS) and Atari games. However, it remains under-explored for vision-based autonomous urban driving navigation (VB-AUDN). VB-AUDN requires a sophisticated agent working safely in structured, changing, and unpredictable environments; otherwise, inappropriate operations may lead to irreversible or catastrophic damages. In this work, we propose a deductive RL (DeRL) to address this challenge. A deduction reasoner (DR) is introduced to endow the agent with ability to foresee the future and to promote policy learning. Specifically, DR first predicts future transitions through a parameterized environment model. Then, DR conducts self-assessment at the predicted trajectory to perceive the consequences of current policy resulting in a more reliable decision-making process. Additionally, a semantic encoder module (SEM) is designed to extract compact driving representation from the raw images, which is robust to the changes of the environment. Extensive experimental results demonstrate that DeRL outperforms the state-of-the-art model-free RL approaches on the public CAR Learning to Act (CARLA) benchmark and presents a superior performance on success rate and driving safety for goal-directed navigation.
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http://dx.doi.org/10.1109/TNNLS.2021.3109284DOI Listing
September 2021

Neuroinflammation Mediates Faster Brachial Plexus Regeneration in Subjects with Cerebral Injury.

Neurosci Bull 2021 Sep 14. Epub 2021 Sep 14.

Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China.

Our previous investigation suggested that faster seventh cervical nerve (C7) regeneration occurs in patients with cerebral injury undergoing contralateral C7 transfer. This finding needed further verification, and the mechanism remained largely unknown. Here, Tinel's test revealed faster C7 regeneration in patients with cerebral injury, which was further confirmed in mice by electrophysiological recordings and histological analysis. Furthermore, we identified an altered systemic inflammatory response that led to the transformation of macrophage polarization as a mechanism underlying the increased nerve regeneration in patients with cerebral injury. In mice, we showed that, as a contributing factor, serum amyloid protein A1 (SAA1) promoted C7 regeneration and interfered with macrophage polarization in vivo. Our results indicate that altered inflammation promotes the regenerative capacity of the C7 nerve by altering macrophage behavior. SAA1 may be a therapeutic target to improve the recovery of injured peripheral nerves.
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http://dx.doi.org/10.1007/s12264-021-00769-7DOI Listing
September 2021

Anti-fouling and protein separation of [email protected] filtration membrane with in-situ grown MnO nanorods.

Chemosphere 2021 Aug 3;286(Pt 2):131756. Epub 2021 Aug 3.

Key Laboratory of Interfacial Physics and Technology, Chinese Academy of Sciences, Shanghai, 201800, China; Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory (SSRF, ZJLab), Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201204, China. Electronic address:

MnO nanorods with controllable scale were grown in the PVDF-g-PMAA modified membrane to form [email protected] MnO membrane through the in situ redox reaction of KMnO solution, which is confirmed by scanning electron microscopy (SEM) and X-ray energy-dispersion spectroscopy (EDX). The pore size of the membrane decreased with the increase of KMnO solution concentration. The thermodynamic stability and the hydrophilicity of the membrane were also enhanced by the MnO nanorods. The water flux, bovine serum albumin (BSA)/Lysozyme protein solution flux and rejection, flux recovery, etc. showed effective improvement of the anti-fouling performance of the [email protected] MnO membrane. More importantly, it can effectively separate BSA from lysozyme, which provided a potential application in the field of biology, food, and other industrial fields for the requirement of separation and purification.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131756DOI Listing
August 2021

Application of simple bladder volume measurement and cystography in rehabilitation training of patients with neurogenic bladder dysfunction.

Asian J Surg 2021 Jul 28. Epub 2021 Jul 28.

Department of Rehabilitation, Taizhou People's Hospital, Taizhou, Jiangsu, 225300, China.

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http://dx.doi.org/10.1016/j.asjsur.2021.07.019DOI Listing
July 2021

Identification of hub genes in rheumatoid arthritis through an integrated bioinformatics approach.

J Orthop Surg Res 2021 Jul 16;16(1):458. Epub 2021 Jul 16.

Department of Rheumatology and Immunology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 32 West of First Ring Road, Chengdu, Sichuan, 610072, P.R. China.

Background: Rheumatoid arthritis (RA) is a common chronic autoimmune disease characterized by inflammation of the synovial membrane. However, the etiology and underlying molecular events of RA are unclear. Here, we applied bioinformatics analysis to identify the key genes involved in RA.

Methods: GSE77298 was downloaded from the Gene Expression Omnibus (GEO) database. We used the R software screen the differentially expressed genes (DEGs). Gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway were analyzed by using the DAVID online tool. The STRING database was used to analyze the interaction of differentially encoded proteins. PPI interaction network was divided into subnetworks using MCODE algorithm and was analyzed using Cytoscape. Gene set enrichment analysis (GSEA) was performed to identify relevant biological functions. qRT-PCR analysis was also performed to verify the expression of identified hub DEGs.

Results: A total of 4062 differentially expressed genes were selected, including 1847 upregulated genes and 2215 downregulated genes. In the biological process, DEGs were mainly concentrated in the fields of muscle filament sliding, muscle contraction, intracellular signal transduction, cardiac muscle contraction, signal transduction, and skeletal muscle tissue development. In the cellular components, DEGs were mainly concentrated in the parts of cytosol, Z disk, membrane, extracellular exosome, mitochondrion, and M band. In molecular functions, DEGs were mainly concentrated in protein binding, structural constituent of muscle, actin binding, and actin filament binding. KEGG pathway analysis shows that DEGs mainly focuses on pathways about lysosome, Wnt/β-catenin signaling pathway, and NF-κB signaling pathway. CXCR3, GNB4, and CXCL16 were identified as the core genes that involved in the progression of RA. By qRT-PCR analysis, we found that CXCR3, GNB4, and CXCL16 were significantly upregulated in RA tissue as compared to healthy controls.

Conclusion: In conclusion, DEGs and hub genes identified in the present study help us understand the molecular mechanisms underlying the progression of RA, and provide candidate targets for diagnosis and treatment of RA.
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http://dx.doi.org/10.1186/s13018-021-02583-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283956PMC
July 2021

Clinical Anatomy of Human Donor C7 Nerve Roots for Surgical Transfer in Patients with Spastic Arm Paralysis.

World Neurosurg 2021 Sep 26;153:e213-e219. Epub 2021 Jun 26.

Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai, China; Limb Function Reconstruction Center, Huashan Hospital, Fudan University, Shanghai, China; National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, China; Department of Hand and Upper Extremity Surgery, Jing'an District Central Hospital, Shanghai, China. Electronic address:

Background: Contralateral C7 (CC7) nerve transfer has successfully restored hand function in patients with spastic hemiplegia from chronic central nervous system injuries. However, little is known about the morphology and anatomy of the donor C7 nerve root in patients undergoing this procedure. This study quantified intraoperative measurements of donor C7 nerve roots during CC7 transfer surgery for spastic hemiplegia in patients treated at a high-volume center to describe observed anatomical variations for successful direct anastomosis.

Methods: A database of images from 21 patients (2 females, 19 males) undergoing CC7 surgery was searched for photographic data that contained a standard ruler measuring donor C7 nerve root length after surgical sectioning and before transfer. Two independent observers analyzed these images and recorded C7 nerve root diameter, length, and branch lengths.

Results: Mean (SD) values of donor C7 nerve measurements were length, 53.5 (8.0) mm; diameter, 5.1 (0.9) mm; branch length following surgical sectioning, 18.3 (6.3) mm. Right-sided donor C7 nerve roots yielded significantly longer branches compared with left-sided donor C7 nerve roots (P = 0.01). Other patient factors such as age, sex, or laterality of brain injury did not influence intraoperative anatomy.

Conclusions: We report detailed intraoperative measurements of the donor C7 root during CC7 nerve transfer for spastic hemiplegia. These findings describe existing variation in surgical C7 nerve root anatomy in patients undergoing this procedure and may serve as a general reference for the expected donor C7 length in successful direct anastomosis.
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http://dx.doi.org/10.1016/j.wneu.2021.06.087DOI Listing
September 2021

DHA-enriched phosphatidylserine ameliorates non-alcoholic fatty liver disease and intestinal dysbacteriosis in mice induced by a high-fat diet.

Food Funct 2021 May;12(9):4021-4033

Department of Aquaculture, Zhejiang Ocean University, Zhoushan 316000, China.

Docosahexaenoic acid-enriched phosphatidylserine (DHA-PS) has attracted increasing attention because of its unique health benefits. In this study, DHA-PS was biosynthesized from DHA-enriched phosphatidylcholine (DHA-PC), which was extracted from herring roe, Clupea harengus. The ameliorating effect of DHA-PS on high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) was investigated using a mouse model. The DHA-PS treatment ameliorated NAFLD and effectively decreased the serum total cholesterol, triglyceride, non-esterified fatty acid, and low-density lipoprotein cholesterol levels and considerably increased the serum high-density lipoprotein cholesterol levels. Moreover, the DHA-PS treatment reduced the levels of liver-function enzymes and pro-inflammatory cytokines and also the oxidative stress indices. Furthermore, DHA-PS increased the diversity and richness of the beneficial intestinal microorganisms, suggesting its potential as a dietary supplement and functional food to combat HFD-induced NAFLD.
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http://dx.doi.org/10.1039/d0fo03471aDOI Listing
May 2021

Occurrence, latitudinal gradient and potential sources of perchlorate in the atmosphere across the hemispheres (31°N to 80°S).

Environ Int 2021 11 8;156:106611. Epub 2021 May 8.

MNR Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China.

Perchlorate (ClO) is harmful to human health, and knowledge on the levels and sources of natural ClO in different environments remains rather limited. Here, we investigate ClO in aerosol samples collected along a cross-hemisphere ship cruise between China and Antarctica and on a traverse between coastal East Antarctica and the ice sheet summit (Dome Argus). Perchlorate concentrations range from a few to a few hundred pg m. A clear latitudinal trend is found, with elevated ClO concentrations near populated areas and in the southern mid-high latitudes. Spatial patterns of atmospheric ClO over oceans near the landmasses support that terrestrial ClO is not transported efficiently over long distances. In the southern mid-latitudes, higher ClO concentrations in March than in November-December may be caused by significant stratospheric inputs in March. Perchlorate concentrations appear to be higher in the warm half than in the cold half of the year in the southern high latitudes, suggesting seasonal difference in main atmospheric sources. ClO may be formed in the reactions between chlorine free radical (Cl·) and ozone (O) in the stratosphere when Antarctic ozone hole occurs during September-October. And the stratosphere-produced ClO is moved to the boundary layer in several months and may be responsible for the high ClO concentrations in the warm half of the year. Perchlorate produced by photochemical reactions between O and Cl· in the Antarctic stratosphere is likely responsible for the higher ClO concentrations in Antarctica than in Arctic.
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http://dx.doi.org/10.1016/j.envint.2021.106611DOI Listing
November 2021

ANCA-associated vasculitis overlaps with systemic sclerosis: a case report and literature review.

Eur J Med Res 2021 Mar 31;26(1):30. Epub 2021 Mar 31.

Department of Rheumatology and Immunology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, No.32 The First Ring Road West 2, Chengdu, 610072, China.

Background: Systemic sclerosis (SSc) and anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) both affect the kidney and may cause renal failure. Treatment of AAV is dramatically different from that of SSc renal crisis (SRC). Kidney biopsy is not recommended for diagnosing SRC, but it is the only reliable diagnostic method for AAV.

Case Presentation: Here, a 49-year-old male patient with diffuse SSc presented with acute renal insufficiency and detectable ANCA with myeloperoxidase-specific antibodies. A renal biopsy revealed necrotizing glomerulonephritis and was consistent with AAV. This finding confirms the existence of AAV and SSc overlap syndrome. The patient was treated with intravenous methylprednisolone, intravenous cyclophosphamide, tandem membrane plasma exchange, and hemodialysis. After treatment, his clinical symptoms remained stable, and his creatinine and C-reactive protein (CRP) levels have remained normalized as of his most recent follow-up after hospital discharge.

Conclusions: AAV can overlap with SSc; although this condition is rare, it is associated with considerable morbidity and mortality. Therefore, it is critical to recognize AAV in the setting of worsening renal function due to SSs and provide appropriate treatment. Several clinical features are suggestive of AAV rather than SRC, but renal biopsy is required for accurate diagnosis.
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http://dx.doi.org/10.1186/s40001-021-00500-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011393PMC
March 2021

Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity.

Chem Commun (Camb) 2021 Feb 28;57(16):2041-2044. Epub 2021 Jan 28.

Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China.

We develop for the first time an aptamer-mediated rolling circle amplification approach for label-free and sensitive detection of histone-modifying enzyme (HME) activity. This method can achieve femtomolar sensitivity for histone acetyltransferase Tip60 assay, which is the most sensitive HME assay reported so far. It can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.
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http://dx.doi.org/10.1039/d0cc07763aDOI Listing
February 2021

Asymmetric Synthesis of -Fissistigmatin C.

Org Lett 2021 01 18;23(1):93-96. Epub 2020 Dec 18.

Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, China.

The asymmetric synthesis of -fissistigmatin C is successively accomplished in 12 steps (longest linear sequence (LLS)). Relying on the enantioselective coupling of aliphatic aldehyde with 2-hydroxychalcone promoted by cooperative organocatalysts, the pivotal linkage of -fissistigmatin C between the flavonoid and the sesquiterpenoid fragment was stereoselectively established. An unprecedented final-stage radical cascade was also featured in this synthesis, which enabled the simultaneous establishment of the -decalin framework via forging two consecutive C-C bonds in one step.
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http://dx.doi.org/10.1021/acs.orglett.0c03766DOI Listing
January 2021

Ticagrelor prevents tumor metastasis via inhibiting cell proliferation and promoting platelet apoptosis.

Anticancer Drugs 2020 11;31(10):1012-1017

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.

Tumor cells can activate platelets, which in turn facilitate tumor cell survival and dissemination. Platelets inhibition or blocking platelet-tumor cell interactions has become a strategy to suppress tumor progression. In this study, we investigated the effect of ticagrelor, a new antiplatelet drug, on tumor cell proliferation and metastasis. Our results show that ticagrelor not only inhibits the proliferation, migration, and invasion of B16F10 and Lewis lung carcinoma cells but also induces platelet apoptosis. In addition, we find that apoptosis of the platelet cells is dose dependent. Further, the result of in-vivo experiments proved that ticagrelor treatment decreased the tumor metastasis. The results of this study demonstrate that ticagrelor may be a potential anti-tumor agent for tumor metastasis.
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http://dx.doi.org/10.1097/CAD.0000000000000925DOI Listing
November 2020

Hyperselective neurectomy in the treatment of elbow and wrist spasticity: an anatomical study and incision design.

Br J Neurosurg 2020 Sep 21:1-6. Epub 2020 Sep 21.

Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai, China.

Objective: Hyperselective neurectomy is used to treat spastic arm paralysis. The aim of the study was to analyze the nerve branching patterns of elbow and wrist flexors/pronator to inform hyperselective neurectomy approached.

Methods: Eighteen upper extremities of fresh cadaver specimen were dissected. The number of motor branches from the musculocutaneous nerve to biceps brachii and brachialis, median nerve to pronator teres, flexor carpi radialis and ulnar nerve to flexor carpi ulnaris were counted. The origin site of each primary motor branch was documented.

Results: Either biceps or brachialis was innervated by one or two primary motor branches. Pronator teres was innervated by one to three motor trunks and the pattern for flexor carpi radialis was a common trunk with other branches. The origin of the biceps and brachialis nerve trunk was located approximately 30% to 60% of the length of the arm. The median nerve branched to pronator teres and flexor carpi radialis at the region about 34mm (SD 18.8mm) above and 50mm (SD 14.9mm) below the medial epicondyle. Flexor carpi ulnaris was innervated by one to three motor trunks and the mean distance from the medial epicondyle to the origin of flexor carpi ulnaris nerve on ulnar nerve was 18.7 mm (SD 6.5mm).

Conclusion: Primary motor branches to elbow flexors, wrist flexors and pronators were various, while the regions of their origins were relatively settled. It was recommended the incisions be designed according to the location of the primary motor trunks.
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http://dx.doi.org/10.1080/02688697.2020.1823939DOI Listing
September 2020

Individuals' math and science motivation and their subsequent STEM choices and achievement in high school and college: A longitudinal study of gender and college generation status differences.

Dev Psychol 2020 Nov 10;56(11):2137-2151. Epub 2020 Sep 10.

School of Education.

Math and science motivational beliefs are essential in understanding students' science, technology, engineering, and math (STEM) achievement and choices in high school and college. Drawing on the Eccles' expectancy-value theory and Arnett's emerging adulthood framework, this study examined the relations among high school students' motivational beliefs in ninth grade and their STEM course taking and grade point average (GPA) throughout high school as well as their STEM major choice in college. In addition, we examined subgroup differences across (a) gender and (b) college generation status by testing mean-level differences as well as whether these relations between math and science motivational beliefs and STEM outcomes varied by gender and college generation status. Using nationally representative data from the High School Longitudinal Study ( = 14,040; = 14; 51% female students), this study found that adolescents' math and science motivational beliefs at the beginning of high school were positively associated with STEM achievement and course taking throughout high school and college major choices 7 years later. The results showed that female and first-generation college students had lower math and science self-concept of ability and were less likely to pursue a STEM major in college. However, in most cases, the relations among indicators did not vary by gender and college generation status. This study provided insights for policymakers and practitioners that gender and college generation gaps in STEM are evident at least by the beginning of high school and carry forward to their STEM college choices. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
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http://dx.doi.org/10.1037/dev0001110DOI Listing
November 2020

Unusual ectopic ACTH syndrome in a patient with orbital neuroendocrine tumor, resulted false-positive outcome of BIPSS:a case report.

BMC Endocr Disord 2020 Jul 31;20(1):116. Epub 2020 Jul 31.

Division of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.

Background: Cushing's syndrome has been described as a complex endocrine disorder characterized with high cortisol concentration. Correct and early diagnosis of Cushing's syndrome is challenging. According to the latest guideline, bilateral inferior petrosal sinus sampling (BIPSS) is considered to be the gold standard for the differential diagnosis. However, in some unusual cases, this method may be false positive. Here, we presented a rare case of orbital neuroendocrine tumor secreting adrenocorticotrophic hormone with false positive inferior petrosal sinus sampling.

Case Presentation: A 48-year-old woman was admitted to West China Hospital of Sichuan University, presenting with fatigue, whole body edema for 1 year, alopecia and skin pigmentation for 5 months. Hormonal profiles including plasma cortisol and adrenocorticotrophic hormone (ACTH) measurements and low-dose dexamethasone inhibition test suggested that the patient had Cushing's syndrome. However, during tumor location phase, the results of high-dose dexamethasone inhibition test (HDDST) contradicted desmopressin (DDAVP) stimulation test. Thus, BIPSS was employed, and its results indicated a pituitary origin. Interestingly, MRI of sellar region showed an innocent pituitary but caught a serendipitous lesion in the lateral rectus muscle of left eye, which was later proved to be an orbital neuroendocrine tumor secreting ACTH by pathological and immunohistochemical results. ACTH level of the patients was < 0.1 ng/L and cortisol level was 51.61 nmol/L 1 week after surgery. At 24 months follow-up, the patient appeared stable with no complaints nor any symptoms of Cushing's syndrome, including moon face, purple striate and central obesity. The patient's life quality also improved significantly.

Conclusion: We reported a rare case of endogenous Cushing's syndrome due to ectopic ACTH secreting from an orbital neuroendocrine tumor. This unique case of orbital EAS suggests that orbital venous blood backflow, owning to abnormal anatomic structures, may possibly lead to false positive BIPSS results.
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http://dx.doi.org/10.1186/s12902-020-00590-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7393836PMC
July 2020

Silencing of circular RNA ANRIL attenuates oxygen-glucose deprivation and reoxygenation-induced injury in human brain microvascular endothelial cells by sponging miR-622.

Biol Res 2020 Jul 2;53(1):27. Epub 2020 Jul 2.

Department of Critical Care Medicine, Taizhou People's Hospital, Taizhou, Jiangsu, 225300, People's Republic of China.

Background: Circular RNA (circRNA) is highly expressed in the brain tissue, but its molecular mechanism in cerebral ischemia-reperfusion remains unclear. Here, we explored the role and underlying mechanisms of circRNA antisense non-coding RNA in the INK4 locus (circ_ANRIL) in oxygen-glucose deprivation and reoxygenation (OGD/R)-induced cell injury.

Results: The expression of circ_ANRIL in OGD/R-induced human brain microvascular endothelial cells (HBMECs) was significantly up-regulated, while that of miR-622 was significantly down-regulated. Overexpression of circ_ANRIL significantly inhibited the proliferation of OGD/R-induced HBMECs and aggravated OGD/R-induced cell apoptosis. Moreover, circ_ANRIL overexpression further increased the secretion of interleukin (IL)-1β, IL-6, tumor necrosis factor-α, and monocyte chemoattractant protein-1 in OGD/R-treated HBMECs. The results of bioinformatics analysis and luciferase reporter assay indicated that circ_ANRIL served as an miR-622 sponge to negatively regulate the expression of miR-622 in OGD/R-treated HBMECs. Additionally, circ_ANRIL silencing exerted anti-apoptotic and anti-inflammatory effects by positively regulating the expression of miR-622. Furthermore, inhibition of OGD/R-induced activation of the nuclear factor (NF)-κB pathway by circ_ANRIL silencing was significantly reversed by treatment with miR-622 inhibitor.

Conclusions: Knockdown of circ_ANRIL improved OGD/R-induced cell damage, apoptosis, and inflammatory responses by inhibiting the NF-κB pathway through sponging miR-622.
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http://dx.doi.org/10.1186/s40659-020-00295-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331154PMC
July 2020

Neuroprotective effects of ZL006 in Aβ-treated neuronal cells.

Neural Regen Res 2020 Dec;15(12):2296-2305

Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University; The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University; Jiangsu Key Laboratory for Molecular Medicine, Nanjing, Jiangsu Province, China.

Amyloid beta (Aβ)-induced neurotoxicity and oxidative stress plays an important role in the pathogenesis of Alzheimer's disease (AD). ZL006 is shown to reduce over-produced nitric oxide and oxidative stress in ischemic stroke by interrupting the interaction of neuronal nitric oxide synthase and postsynaptic density protein 95. However, few studies are reported on the role of ZL006 in AD. To investigate whether ZL006 exerted neuroprotective effects in AD, we used Aβ to treat primary cortical neurons and N2a neuroblastoma cells as an in vitro model of AD. Cortical neurons were incubated with ZL006 or dimethyl sulfoxide for 2 hours and treated with Aβ or NH•HO for another 24 hours. The results of cell counting Kit-8 (CCK-8) assay and calcein-acetoxymethylester/propidium iodide staining showed that ZL006 pretreatment rescued the neuronal death induced by Aβ. Fluorescence and western blot assay were used to detect oxidative stress and apoptosis-related proteins in each group of cells. Results showed that ZL006 pretreatment decreased neuronal apoptosis and oxidative stress induced by Aβ. The results of CCK8 assay showed that inhibition of Akt or NF-E2-related factor 2 (Nrf2) in cortical neurons abolished the protective effects of ZL006. Moreover, similar results were also observed in N2a neuroblastoma cells. ZL006 inhibited N2a cell death and oxidative stress induced by Aβ, while inhibition of Akt or Nrf2 abolished the protective effect of ZL006. These results demonstrated that ZL006 reduced Aβ-induced neuronal damage and oxidative stress, and the mechanisms might be associated with the activation of Akt/Nrf2/heme oxygenase-1 signaling pathways.
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http://dx.doi.org/10.4103/1673-5374.285006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749460PMC
December 2020

Ameliorate effect of pyrroloquinoline quinone against cyclophosphamide-induced nephrotoxicity by activating the Nrf2 pathway and inhibiting the NLRP3 pathway.

Life Sci 2020 Sep 3;256:117901. Epub 2020 Jun 3.

College of Food Science and Technology, Bohai University, No. 19 Keji Road, Jinzhou 121013, China.. Electronic address:

Aims: Cyclophosphamide (CTX) is an effective anti-tumor and immunosuppressive agent, but it induces nephrotoxicity in clinical applications. The present study aimed to evaluate the protective effect of pyrroloquinoline quinone (PQQ) on CTX-induced nephrotoxicity.

Main Methods: We injected male ICR mice with CTX (80 mg/kg/day), and determined nephrotoxicity indices, MDA and antioxidant defenses, inflammatory cytokines, and the levels of main proteins in the Nrf2-HO-1 and NLRP3 signaling pathways.

Key Findings: PQQ has significantly decreased the serum levels of creatinine and urea compared to Model group. When treated with PQQ, MDA, IL-1β, IL-6, and TNF-α levels have decreased, and SOD, GSH-Px, and CAT activity have increased in the kidney tissues of CTX-induced mice. PQQ activated the Nrf2-mediated signaling pathway, as indicated by the increased expression of Nrf2, HO-1, GCLM, and NQO1. Moreover, PQQ inhibited the NLRP3 inflammatory pathway, as indicated by the reduced expression of NLRP3, ASC, and Caspase-1.

Significance: Our results suggest that PQQ protects against CTX-induced nephrotoxicity, probably by activating the Nrf2-mediated antioxidant pathway and inhibiting the NLRP3 inflammatory pathway.
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http://dx.doi.org/10.1016/j.lfs.2020.117901DOI Listing
September 2020

Sauchinone attenuates inflammatory responses in dendritic cells via Blimp-1 and ameliorates dextran sulfate sodium (DSS)-induced colitis.

Biochem Biophys Res Commun 2020 07 16;527(4):902-908. Epub 2020 May 16.

Clinical Immunology Translational Medicine Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Department of Gastroenterology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. Electronic address:

Inflammatory bowel disease (IBD) is a complex inflammatory disorder of the digestive tract with dysregulated innate and adaptive immune responses. Dendritic cells (DC), the most important antigen presenting cells, act as bridges connecting the adaptive and innate immune systems, and play a crucial role in the regulation of local homeostasis in the gut and are also essential mediators in the initiation and development of intestinal inflammation. Our recent study found that sauchinone (SAU) was able to ameliorate experimental colitis in mice by restraining Th17 cell differentiation and their pathogenicity. Here, we found that SAU significantly inhibited LPS-induced DC activation. Moreover, SAU suppressed the ability of LPS-primed DC to induce Th1/Th17 cell differentiation, but SAU-treated DC up-regulated their ability to initiate Foxp3 Treg cell generation. Of note, we found that genetical ablation of Blimp-1 in DC markedly abrogated the SAU suppression of pro-inflammatory cytokine or promote immunomodulatory molecule production by DC. Blimp-1 deficiency boosted the ability of DC to polarize naïve CD4 T cells into Th1/Th17 cell lineages. SAU failed to alleviated DSS-induced colitis in mice with Blimp-1-deficient DC. Our results shed new lights on the mechanisms of how SAU regulates DC biology and intestinal inflammation.
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http://dx.doi.org/10.1016/j.bbrc.2020.05.022DOI Listing
July 2020

SiRNA-directed self-assembled quantum dot biosensor for simultaneous detection of multiple microRNAs at the single-particle level.

Biosens Bioelectron 2020 Jun 26;157:112177. Epub 2020 Mar 26.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan, 250014, China. Electronic address:

MicroRNAs are essential post-transcriptional regulators and may act as the noninvasive biomarkers for disease diagnosis. Sensitive and multiplexed detection of microRNAs may facilitate the accurate and early clinical diagnosis, but the available methods are usually compromised by using organic dyes as the signal probes, laborious chemical and enzymatic manipulations, and the complicated reaction schemes. Here we reported a siRNA-directed self-assembled quantum dot (QD) biosensor for facile and simultaneous detection of multiple microRNAs. In this biosensor, the binding of microRNA targets with corresponding QD nanoprobes leads to the formation of siRNA duplexes, which not only induces the spectrally resolved coding of microRNAs, but also facilitates the assembly of QDs:magnetic nanoparticle bioconjugates for the isolation and enrichment of target microRNAs. The disassembled QDs can be sensitively detected by single-molecule detection, enabling quantitatively sensing of microRNAs at the single-particle level. This biosensor employs only QDs as the signal reporters, which can simultaneously detect multiple microRNAs from the same sample and achieves femtomolar sensitivity and single-base mismatch selectivity without the involvement of any target labeling and amplification steps. Moreover, it can be successfully applied for simultaneous detection of circulating microRNAs in clinical serum samples, holding great potential in non-invasive early diagnosis and biomedical researches.
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http://dx.doi.org/10.1016/j.bios.2020.112177DOI Listing
June 2020

The promoting effect of MMP13 on mediating the development of HFLS-RA by the target of miR-19a through IL-17 signaling pathway.

J Cell Biochem 2020 10 21;121(10):4282-4294. Epub 2020 Jan 21.

Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu, China.

By investigating the expression profiles of miR-19a and metalloproteinases (MMP13) in human fibroblast-like synoviocytes-rheumatoid arthritis (HFLS-RA) and HFL cells lines, this study intends to confirm the directly target connection between them and reveal the effect of suppressing MMP13 on HLFS-RA migration, invasion and apoptosis. After screening the abnormal expressed messenger RNAs and microRNAs in synovial tissues of patients with RA, the underlying pathway was determined by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The HFLS-RA cell line was transfected for the following experiments with pcDNA3.1(+) served as vector. The directly target association between miR-19a and MMP13 was confirmed by Luciferase reporter assay. Microarray analysis suggested that MMP13 was upregulated while miR-19a was downregulated in HFLS of RA tissues compared with the healthy control group. MMP13 was related to many proteins in protein-protein interaction network, which might be the main influencing factor of RA. KEGG pathway analysis identified that interleukin (IL)-17 pathway was activated in the regulation of MMP13 in the development of RA. Through observing the alteration of luciferase activity, miR-19a could indeed bind to the 3'UTR of the downstream of MMP13, the target association was then confirmed. The proliferation and invasion of HFLS-RA were promoted by overexpressing MMP13 protein. miR-19a could function as a suppressor of MMP13 and thereby retard the severity of RA. The results showed that miR-19a could regulate the expression of MMP13 in HFLS-RA by mediating the proliferation and invasion of HFLS-RA through IL-17 signaling pathway, thereby participating in the degradation of chondrocytes in the progression of RA.
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http://dx.doi.org/10.1002/jcb.29609DOI Listing
October 2020

Brief report: Patterns of prosocial behaviors in middle childhood predicting peer relations during early adolescence.

J Adolesc 2020 01 29;78:1-8. Epub 2019 Nov 29.

School of Education, University of California Irvine, 200 Education Bldg, Irvine, CA, 92697, USA. Electronic address:

Introduction: Early adolescence is a developmental period in which peer victimization, bullying, relational aggression, and social exclusion are particularly prominent. As these behaviors have long-term implications for children, in this study, we investigated early variations of prosocial behaviors as one of the critical precedents that shape youth's subsequent peer relational outcomes. Specifically, we identified different profiles of prosocial behaviors in middle childhood (Grade 4) and related these profiles to peer relationships in early adolescence (Grade 6).

Method: Using longitudinal data from the NICHD Study of Early Child Care and Youth Development (N = 914; 52% girls), a three-step latent profile analysis was conducted on Grade 4 prosocial behaviors, which were then related to Grade 6 teacher- and student-rated peer relationships (e.g., relational aggression).

Results: Four patterns of prosocial behaviors emerged: low prosocial (18%), high prosocial (67%), primarily friendly (8%), and primarily kind (7%). These four patterns of prosocial behaviors were differentially related to later problematic peer relationships: Low prosocial youth demonstrated the most problematic peer relationships (Mdiff = 0.36-0.93, all p < .001). Primarily friendly (but not kind) youth displayed more general and relational aggression (Mdiff = 0.22, SE = 0.07, p = .002; and Mdiff = 0.18, SE = 0.06, p = .006, respectively).

Conclusion: Both low prosocial and primarily friendly youth are at risk for displaying peer-related problems; interventions that build prosocial behaviors in youth with a low prosocial or primarily friendly profile may help prevent problematic peer relationships at early adolescence.
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http://dx.doi.org/10.1016/j.adolescence.2019.11.004DOI Listing
January 2020

Contralateral cervical seventh nerve transfer for spastic arm paralysis via a modified prespinal route: a cadaveric study.

Acta Neurochir (Wien) 2020 01 18;162(1):141-146. Epub 2019 Nov 18.

Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China.

Background: We proposed contralateral cervical seventh nerve transfer for spastic arm paralysis after central neurological injury in the New England Journal of Medicine (NEJM) in 2018. In this surgery, we applied a new surgical route for nerve transfer, the Huashan prespinal route. The objective of this study was to elaborate our new surgical technique, clarify its relationship to the vertebral artery, and provide anatomical data on this novel method.

Methods: The effectiveness and safety of the Huashan prespinal route in contralateral C7 nerve transfer were evaluated anatomically. Nine cadavers (4 males, 5 females) were available for this study. Among these, anatomical parameters of the vertebral artery were obtained from 6 cadavers, and the anastomosis of the bilateral cervical seventh nerve was observed on 3 cadavers undergoing contralateral C7 nerve transfer via the Huashan prespinal route.

Results: Tension-free anastomosis of the bilateral cervical seventh nerve was achieved through the Huashan prespinal route. The tilt angle of the vertebral artery to the sagittal plane (with thyroid cartilage as the origin) was 25.5 ± 4.5°, at 22.5 ± 1.6° and 28.7 ± 4.3° on the left and right side, respectively. The safe drilling angle to penetrate through the longus colli muscles for the creation of a longus colli muscle tunnel to avoid injury to the vertebral artery in our surgical technique was above 33.2°.

Conclusions: The cadaveric study confirms that the presented technique allowed simple, effective, and safe contralateral C7 nerve transfer. This technique can be used in the treatment of hemiplegia and brachial plexus injury. There is a safe scope of drilling angle for creating the longus colli muscle tunnel required for this surgical route. The anatomical parameters obtained in this study will be helpful for the performance of this operation.
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http://dx.doi.org/10.1007/s00701-019-04069-yDOI Listing
January 2020

The Recognition of the Distribution Features of Corticospinal Neurons by a Retrograde Trans-synaptic Tracing to Elucidate the Clinical Application of Contralateral Middle Trunk Transfer.

Neuroscience 2020 01 31;424:86-101. Epub 2019 Oct 31.

Department of Hand and Upper Extremity Surgery, Jing'an District Central Hospital of Shanghai, Fudan University, Shanghai 200040, China; Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China; Institutes of Brain Science, Fudan University, Shanghai 200032, China; State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center of Brain Science, Fudan University, Shanghai 200032, China; Priority Among Priorities of Shanghai Municipal Clinical Medicine Center, Shanghai 200040, China; National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China. Electronic address:

Corticospinal neurons (CSNs) undertake direct cortical outputs to the spinal cord and innervate the upper limb through the brachial plexus. Our previous study has shown that the contralateral middle trunk transfer to the paralyzed upper extremity due to cerebral injury can reconstruct the functional cerebral cortex and improve the function of the paralyzed upper extremity. To interpret the cortical reconstruction and the motor improvement after the middle trunk transfer, we explored the distribution of CSNs connecting to the middle, upper, and lower trunk of the brachial plexus by retrograde trans-neuronal tracing using pseudorabies virus (PRV-EGFP or PRV-mRFP). We show that, rather than an individual specific area, these CSNs labelled by each trunk of the brachial plexus were widespread and mainly assembled within the primary motor cortex (M1), secondary motor cortex (M2), primary somatosensory cortex (S1), and slightly within the secondary somatosensory cortex (S2). The three trunk-labelled CSNs were intermingled in these cortices, and mostly connected to more than two trunks, especially the middle trunk-labelled CSNs with higher proportion of co-labelled neurons. Our findings revealed the distribution features of CSNs connecting to the adjacent spinal nerves that innervate the upper limb, which can improve our understanding of the corticospinal circuits associated with motor improvement and the functional cortical reconstruction after the middle trunk transfer.
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http://dx.doi.org/10.1016/j.neuroscience.2019.09.030DOI Listing
January 2020

Letter to the Editor Regarding "Treatment of Central Paralysis of Upper Extremity Using Contralateral C7 Nerve Transfer via Posterior Spinal Route".

World Neurosurg 2019 08;128:618-619

Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai, China; The National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, China; Department of Hand and Upper Extremity Surgery, Jing'an District Central Hospital, Fudan University, Shanghai, China. Electronic address:

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http://dx.doi.org/10.1016/j.wneu.2019.03.253DOI Listing
August 2019

Sudden sensorineural hearing loss with end-stage renal disease: a report of 32 cases.

Acta Otolaryngol 2019 Nov 5;139(11):1004-1007. Epub 2019 Sep 5.

Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Xin Jiang Medical University , Urumchi , China.

Patients with end-stage renal disease (ESRD) have a higher risk of suffering sudden sensorineural hearing loss (SSNHL), but little is known about this population. To investigate the clinical characteristics, treatment and recovery of SSNHL patients with ESRD. Records of 32 SSNHL patients with ESRD were reviewed, including clinical characteristics and hearing recovery. Patients were divided into intratympanic steroid (ITS) group and oral steroid (OS) group, and hearing recovery was compared between two groups. Twenty-six patients (81.3%) exhibited tinnitus, and 18 patients (56.3%) suffered vertigo. Mean pure-tone threshold at the initial presentation was 73.2 ± 19.4 dB, and the audiogram configuration was ascending in 9.4%, descending in 9.4%, flat in 34.4% and profound in 46.9% cases. At 3-month follow-up, percentages of patients in complete recovery, partial recovery, slight recovery, and no improvement were 18.8%, 31.3%, 21.9% and 28.1%, respectively. Furthermore, the overall recovery rate, complete recovery rate and hearing improvement were significantly higher in the ITS group than those in the OS group. SSNHL patient with ESRD often suffered a severe hearing loss with a high rate of accompanying tinnitus and vertigo. ITS may provide better audiological results for SSNHL with ESRD than OS.
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http://dx.doi.org/10.1080/00016489.2019.1659516DOI Listing
November 2019

The effectiveness of treatments for Kashin-Beck disease: a systematic review and network meta-analysis.

Clin Rheumatol 2019 Dec 2;38(12):3595-3607. Epub 2019 Aug 2.

Academic Rheumatology, Division of Rheumatology, Orthopedics and Dermatology, School of Medicine, Nottingham City Hospital, University of Nottingham, Room A18, Hucknall Road, Nottingham, NG5 1PB, UK.

Objectives: This study aims to evaluate the efficacy of treatments for Kashin-Beck disease (KBD).

Method: We searched PubMed, Cochrane Central Register of Controlled Trials, Embase, Web of Science, SinoMed, Chinese National Knowledge Infrastructure, reference lists and published systematic reviews and registries of ongoing trials through May 2015 for randomised controlled trials (RCTs) of treatments for KBD. Outcomes of interest were pain, function, stiffness, overall clinical improvement, radiographic improvement (X-ray) and adverse events. Frequentist network meta-analyses were conducted using random-effects consistency model to assess the efficacy of treatments for KBD.

Results: Forty-four RCTs with 9815 participants were included in the review. In children or adolescents, selenium (risk ratio 1.88, 95% confidence interval (CI) 1.51-2.33), vitamin C (2.03, 1.40-2.95) and aspirin (2.14, 1.12-4.08) were effective for radiographic structure improvement. In adults, chondroitin plus glucosamine was the best for pain (standardised mean difference 1.46, 95% CI 1.07-1.85), followed by intra-articular injection of hyaluronic acid (IAH) (1.09, 0.70-1.48), chondroitin (0.84, 0.47-1.21), diclofenac (0.63, 1.18-1.08), naproxen (0.55, 0.12-0.98), meloxicam (0.52, 0.03-1.01) and glucosamine (0.40, 0.13-0.67) compared to placebo, with similar results for other clinical outcomes in adults. However, the strength of most evidence was limited by the small number of trials with low to moderate quality.

Conclusions: Selenium supplement has demonstrated some benefits for structural improvement of the disease in children. Chondroitin, glucosamine, IAH and nonsteroid anti-inflammatory drugs are effective for symptom improvements of KBD in adults. Evidence of surgical and complementary treatments for symptoms and aspirin and vitamin C for structure has yet to be established.Key Points• There were 23 nutraceuticals, pharmaceuticals and surgical and complementary treatments assessed for Kashin-Beck disease (KBD) in randomised trials.• Among the 23 treatments, chondroitin, glucosamine, IAH and non-steroid anti-inflammatory drugs are more effective than placebo to relieve symptoms for adults with KBD.• Selenium supplement is more effective than placebo for radiographic improvement in children or adolescents.• The efficacy of surgeries, aspirin, vitamin C and complementary treatments for KBD has not been established yet.
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http://dx.doi.org/10.1007/s10067-019-04704-0DOI Listing
December 2019

A shape-memory and spiral light-emitting device for precise multisite stimulation of nerve bundles.

Nat Commun 2019 06 26;10(1):2790. Epub 2019 Jun 26.

State Key Laboratory of Medical Neurobiology and MOE Frontier Center for Brain Science, Institutes of Brain Science, Department of Hand Surgery, National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, 200433, Shanghai, China.

We previously demonstrated that for long-term spastic limb paralysis, transferring the seventh cervical nerve (C7) from the nonparalyzed side to the paralyzed side results in increase of 17.7 in Fugl-Meyer score. One strategy for further improvement in voluntary arm movement is selective activation of five target muscles innervated by C7 during recovery process. In this study, we develop an implantable multisite optogenetic stimulation device (MOSD) based on shape-memory polymer. Two-site stimulation of sciatic nerve bundles by MOSD induces precise extension or flexion movements of the ankle joint, while eight-site stimulation of C7 nerve bundles induce selective limb movement. Long-term implant of MOSD to mice with severed and anastomosed C7 nerve is proven to be both safe and effective. Our work opens up the possibility for multisite nerve bundle stimulation to induce highly-selective activations of limb muscles, which could inspire further applications in neurosurgery and neuroscience research.
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http://dx.doi.org/10.1038/s41467-019-10418-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594927PMC
June 2019

Dissection of the Genetic Architecture of Rice Tillering using a Genome-wide Association Study.

Rice (N Y) 2019 Jun 20;12(1):43. Epub 2019 Jun 20.

State Key Laboratory for Biology of Plant Diseases and Insect Pest, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Background: Rice tiller number (TN) is one of the most important components associated with rice grain yield. Around one hundred rice TN genes have been identified, but dissecting the genetic architecture of rice TN variations remains difficult because of its complex trait and control by both major genes and quantitative trait loci (QTLs).

Results: In this study, we used a subset of the rice diversity population II (S-RDP-II), genotyped with 700,000 single nucleotide polymorphisms (SNPs), to identify the loci associated with tiller number variations (LATNs) through a genome-wide association study (GWAS). The analysis revealed that 23 LATNs are significantly associated with TN variations. Among the 23 LATNs, eight are co-localized with previously cloned TN genes, and the remaining 15 LATNs are novel. DNA sequence analysis of the 15 novel LATNs led to the identification of five candidate genes using the accessions with extreme TN phenotypes. Genetic variations in two of the genes are mainly located in the promoter regions. qRT-PCR analysis showed that the expression levels of these two genes are also closely associated with TN variations.

Conclusions: We identified 15 novel LATNs that contribute significantly to the genetic variation of rice TN. Of these 15, the five identified TN-associated candidate genes will enhance our understanding of rice tillering and can be used as molecular markers for improving rice yield.
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http://dx.doi.org/10.1186/s12284-019-0302-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586736PMC
June 2019

The re-emerging of SADS-CoV infection in pig herds in Southern China.

Transbound Emerg Dis 2019 Sep 10;66(5):2180-2183. Epub 2019 Jul 10.

College of Animal Science, South China Agricultural University, Guangzhou, China.

A new highly virulent swine acute diarrhoea syndrome coronavirus (SADS-CoV) emerged in Guangdong province in 2017 followed by fatal diarrhoea that involved the death of 24,693 piglets. And yet from May 2017 to January 2019, there were no new SADS cases arising in pig herds in Guangdong. In this study, we reported the recent diarrhoea outbreak of SADS-CoV in Southern China on February 2019. Intestinal samples collected from diarrhoeal piglets were detected for common swine virus and confirmed that SADS-CoV was responsible for the diarrhoea case. Meanwhile, serological investigation of sows' sera implied that SADS-CoV has existed in the farm and PEDV antibody may not directly contribute to the amplification of SADS-CoV. Homology and phylogenetic analysis of the whole genome showed that the re-emerging SADS-CoV strain shared high sequence identities with existing SADS-CoV strains and all strains clustered together in Alpha coronavirus. All in all, the report herein emphasized the re-emerging of SADS-CoV and highlights continuous monitoring for this virus.
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http://dx.doi.org/10.1111/tbed.13270DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168562PMC
September 2019
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