Publications by authors named "Stephen J Tucker"

68Publications

Electric Field Induced Wetting of a Hydrophobic Gate in a Model Nanopore Based on the 5-HT Receptor Channel.

ACS Nano 2020 08 23;14(8):10480-10491. Epub 2020 Jul 23.

Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom.

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August 2020

Altered functional properties of a missense variant in the TRESK K channel (KCNK18) associated with migraine and intellectual disability.

Pflugers Arch 2020 07 12;472(7):923-930. Epub 2020 May 12.

Department of Physiology & Biochemistry, Faculty of Medicine and Surgery, University of Malta, MSD, Msida, 2080, Malta.

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July 2020

Induced Polarization in Molecular Dynamics Simulations of the 5-HT Receptor Channel.

J Am Chem Soc 2020 05 8;142(20):9415-9427. Epub 2020 May 8.

Department of Biochemistry, University of Oxford, Oxford OX1 3QU, U.K.

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May 2020

Structure and assembly of calcium homeostasis modulator proteins.

Nat Struct Mol Biol 2020 02 27;27(2):150-159. Epub 2020 Jan 27.

WM Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.

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February 2020

Selectivity filter instability dominates the low intrinsic activity of the TWIK-1 K2P K channel.

J Biol Chem 2020 01 5;295(2):610-618. Epub 2019 Dec 5.

Clarendon Laboratory, Department of Physics, University of Oxford, Oxford OX1 3PU, United Kingdom

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January 2020

A heuristic derived from analysis of the ion channel structural proteome permits the rapid identification of hydrophobic gates.

Proc Natl Acad Sci U S A 2019 07 24;116(28):13989-13995. Epub 2019 Jun 24.

Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom;

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July 2019

CHAP: A Versatile Tool for the Structural and Functional Annotation of Ion Channel Pores.

J Mol Biol 2019 08 17;431(17):3353-3365. Epub 2019 Jun 17.

Clarendon Laboratory, Department of Physics, University of Oxford, UK; OXION Initiative in Ion Channels and Disease, University of Oxford, UK. Electronic address:

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August 2019

Asymmetric mechanosensitivity in a eukaryotic ion channel.

Proc Natl Acad Sci U S A 2017 10 18;114(40):E8343-E8351. Epub 2017 Sep 18.

Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, United Kingdom;

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October 2017

The effects of stretch activation on ionic selectivity of the TREK-2 K2P K channel.

Channels (Austin) 2017 Sep 19;11(5):482-486. Epub 2017 Jul 19.

a Clarendon Laboratory, Department of Physics and OXION Initiative in Ion Channels and Disease , University of Oxford , Oxford , UK.

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September 2017

Dynamic role of the tether helix in PIP-dependent gating of a G protein-gated potassium channel.

J Gen Physiol 2017 08 7;149(8):799-811. Epub 2017 Aug 7.

Fishberg Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY

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August 2017

Bilayer-Mediated Structural Transitions Control Mechanosensitivity of the TREK-2 K2P Channel.

Structure 2017 05 6;25(5):708-718.e2. Epub 2017 Apr 6.

Clarendon Laboratory, Department of Physics, University of Oxford, Oxford OX1 3PU, UK; OXION Initiative in Ion Channels and Disease, University of Oxford, Oxford OX1 3PT, UK. Electronic address:

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May 2017

A BEST example of channel structure annotation by molecular simulation.

Channels (Austin) 2017 Jul 20;11(4):347-353. Epub 2017 Mar 20.

a Department of Biochemistry , University of Oxford , Oxford , UK.

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July 2017

Functional Annotation of Ion Channel Structures by Molecular Simulation.

Structure 2016 12 17;24(12):2207-2216. Epub 2016 Nov 17.

Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK; OXION Initiative in Ion Channels and Disease, University of Oxford, Oxford, UK. Electronic address:

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December 2016

Solution-Based Single-Molecule FRET Studies of K(+) Channel Gating in a Lipid Bilayer.

Biophys J 2016 Jun;110(12):2663-2670

Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, United Kingdom. Electronic address:

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June 2016

Polymodal activation of the TREK-2 K2P channel produces structurally distinct open states.

J Gen Physiol 2016 06;147(6):497-505

Clarendon Laboratory, Department of Physics, University of Oxford, Oxford OX1 3PU, England, UK OXION Initiative in Ion Channels and Disease, University of Oxford, Oxford OX1 3PU, England, UK

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June 2016

Dominant-Negative Effect of a Missense Variant in the TASK-2 (KCNK5) K+ Channel Associated with Balkan Endemic Nephropathy.

PLoS One 2016 26;11(5):e0156456. Epub 2016 May 26.

Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, United Kingdom.

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July 2017

Modular Design of the Selectivity Filter Pore Loop in a Novel Family of Prokaryotic 'Inward Rectifier' (NirBac) channels.

Sci Rep 2015 Oct 16;5:15305. Epub 2015 Oct 16.

Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, United Kingdom.

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October 2015

Crystal Structures of the Extracellular Domain from PepT1 and PepT2 Provide Novel Insights into Mammalian Peptide Transport.

Structure 2015 Oct 27;23(10):1889-1899. Epub 2015 Aug 27.

Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK; OXION Initiative in Ion Channels and Membrane Transport, University of Oxford OX1 3PU, UK. Electronic address:

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October 2015

De novo point mutations in patients diagnosed with ataxic cerebral palsy.

Brain 2015 Jul 16;138(Pt 7):1817-32. Epub 2015 May 16.

4 Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford OX3 9DU, UK 16 Department of Clinical Genetics, Churchill Hospital, Oxford University Hospitals NHS Trust, Oxford, OX3 7LJ, UK

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July 2015

Molecular simulation studies of hydrophobic gating in nanopores and ion channels.

Biochem Soc Trans 2015 Apr;43(2):146-50

*Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.

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April 2015

Novel phenotype associated with a mutation in the KCNA1(Kv1.1) gene.

Front Physiol 2014 15;5:525. Epub 2015 Jan 15.

Department of Neurology, Friedrich-Baur-Institute, Ludwig-Maximilians-University Munich, Germany ; German Network for Mitochondrial Disorders (mitoNET) Ludwigshafen, Germany ; DZNE - German Center for Neurodegenerative Diseases Munich, Germany ; German Center for Vertigo and Balance Disorders Munich, Germany.

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February 2015

Influence of lipids on the hydrophobic barrier within the pore of the TWIK-1 K2P channel.

Channels (Austin) 2015 ;9(1):44-9

a Clarendon Laboratory, Department of Physics; University of Oxford ; Oxford , UK.

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December 2015

Hydrophobic gating in ion channels.

J Mol Biol 2015 Jan 12;427(1):121-30. Epub 2014 Aug 12.

Clarendon Laboratory, Department of Physics, University of Oxford, Oxford OX1 3PU, UK; OXION Initiative in Ion Channels and Disease, University of Oxford, Oxford OX1 2JD, UK. Electronic address:

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January 2015

A hydrophobic barrier deep within the inner pore of the TWIK-1 K2P potassium channel.

Nat Commun 2014 Jul 8;5:4377. Epub 2014 Jul 8.

1] Clarendon Laboratory, Department of Physics, University of Oxford, Oxford OX1 3PU, UK [2] OXION Initiative in Ion Channels and Disease, University of Oxford, Oxford OX1 3PT, UK.

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July 2014

State-dependent network connectivity determines gating in a K+ channel.

Structure 2014 Jul 26;22(7):1037-46. Epub 2014 Jun 26.

Physiological Institute, Christian-Albrechts University, 24118 Kiel, Germany. Electronic address:

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July 2014

Influence of the N terminus on the biophysical properties and pharmacology of TREK1 potassium channels.

Mol Pharmacol 2014 May 7;85(5):671-81. Epub 2014 Feb 7.

Medway School of Pharmacy, University of Kent, Chatham Maritime, Kent, United Kingdom (E.L.V., E.A.-M., N.B., A.M.); Pfizer Neusentis, Great Abington, Cambridge, United Kingdom (K.O., L.C., E.B.S.); and Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, United Kingdom (S.J.T.).

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May 2014

Control of KirBac3.1 potassium channel gating at the interface between cytoplasmic domains.

J Biol Chem 2014 Jan 20;289(1):143-51. Epub 2013 Nov 20.

From the Biological Physics Group, Clarendon Laboratory, University of Oxford, Oxford OX1 3PU, United Kingdom.

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January 2014

Simulation-based prediction of phosphatidylinositol 4,5-bisphosphate binding to an ion channel.

Biochemistry 2013 Jan 28;52(2):279-81. Epub 2012 Dec 28.

Department of Biochemistry, University of Oxford , Oxford OX1 3QU, United Kingdom.

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January 2013

Renal phenotype in mice lacking the Kir5.1 (Kcnj16) K+ channel subunit contrasts with that observed in SeSAME/EAST syndrome.

Proc Natl Acad Sci U S A 2011 Jun 1;108(25):10361-6. Epub 2011 Jun 1.

Université Pierre et Marie Curie Paris 6, Université Paris 5, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche en Santé 872, 75006 Paris, France.

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June 2011

In vitro reconstitution of eukaryotic ion channels using droplet interface bilayers.

J Am Chem Soc 2011 Jun 27;133(24):9370-5. Epub 2011 May 27.

Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, United Kingdom.

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June 2011

Respiratory responses to hypercapnia and hypoxia in mice with genetic ablation of Kir5.1 (Kcnj16).

Exp Physiol 2011 Apr 14;96(4):451-9. Epub 2011 Jan 14.

Department of Surgery and Cancer, Biophysics Section, Imperial College London, London, UK.

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April 2011

Genetic inactivation of Kcnj16 identifies Kir5.1 as an important determinant of neuronal PCO2/pH sensitivity.

J Biol Chem 2011 Jan 3;286(1):192-8. Epub 2010 Nov 3.

Section of Human Physiology, University of Perugia School of Medicine, Perugia 06100, Italy.

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January 2011

Random mutagenesis screening indicates the absence of a separate H(+)-sensor in the pH-sensitive Kir channels.

Channels (Austin) 2010 Sep-Oct;4(5):390-7. Epub 2010 Sep 1.

Department of Physiology, Anatomy and Genetics, University of Oxford, UK.

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May 2011

Kir5.1 underlies long-lived subconductance levels in heteromeric Kir4.1/Kir5.1 channels from Xenopus tropicalis.

Biochem Biophys Res Commun 2009 Oct 8;388(3):501-5. Epub 2009 Aug 8.

Clarendon Laboratory, Department of Physics, University of Oxford, Oxford OX1 3PU, United Kingdom.

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October 2009

Contribution of the central hydrophobic residue in the PXP motif of voltage-dependent K+ channels to S6 flexibility and gating properties.

Channels (Austin) 2009 Jan-Feb;3(1):39-45. Epub 2009 Jan 3.

Section of Human Physiology, Department of Internal Medicine, University of Perugia School of Medicine, Perugia, Italy.

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May 2009

Genetic selection of activatory mutations in KcsA.

Channels (Austin) 2008 Nov-Dec;2(6):413-8. Epub 2008 Nov 27.

Oxford Centre for Gene Function, Department of Physiology Anatomy and Genetics, University of Oxford, Oxford, UK.

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April 2009

Peptide backbone mutagenesis of putative gating hinges in a potassium ion channel.

Chembiochem 2008 Jul;9(11):1725-8

Oxford Centre for Gene Function, Department of Physiology, Anatomy and Genetics, Sherrington Building, Parks Road, Oxford, OX13PT, UK.

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July 2008

How highly charged anionic lipids bind and regulate ion channels.

J Gen Physiol 2008 May 14;131(5):431-8. Epub 2008 Apr 14.

Oxford Centre for Gene Function, Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK.

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May 2008

H bonding at the helix-bundle crossing controls gating in Kir potassium channels.

Neuron 2007 Aug;55(4):602-14

Institute of Physiology II, Friedrich Schiller University, D-07743 Jena, Germany.

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August 2007

Non-equivalent role of TM2 gating hinges in heteromeric Kir4.1/Kir5.1 potassium channels.

Eur Biophys J 2008 Feb 27;37(2):165-71. Epub 2007 Jul 27.

Department of Physiology Anatomy and Genetics, University of Oxford, Oxford, UK.

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February 2008

Molecular dynamics simulations of inwardly rectifying (Kir) potassium channels: a comparative study.

Biochemistry 2007 Mar 28;46(12):3643-52. Epub 2007 Feb 28.

Department of Biochemistry, University of Oxford, UK.

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March 2007

Cloning and functional characterization of a superfamily of microbial inwardly rectifying potassium channels.

Physiol Genomics 2006 Jun 4;26(1):1-7. Epub 2006 Apr 4.

Oxford Centre for Gene Function, Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom.

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June 2006

Long chain CoA esters as competitive antagonists of phosphatidylinositol 4,5-bisphosphate activation in Kir channels.

J Biol Chem 2005 Sep 24;280(35):30760-7. Epub 2005 Jun 24.

Institute of Physiology II, Friedrich Schiller University Jena, Teichgraben 8, 07743 Jena, Germany.

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September 2005

Functional characterisation of missense variations in the Kir4.1 potassium channel (KCNJ10) associated with seizure susceptibility.

Brain Res Mol Brain Res 2005 Sep;139(1):178-83

Oxford Centre for Gene Function, University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, UK.

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September 2005

Identification of a heteromeric interaction that influences the rectification, gating, and pH sensitivity of Kir4.1/Kir5.1 potassium channels.

J Biol Chem 2003 Oct 15;278(44):43533-40. Epub 2003 Aug 15.

Istituto di Ricerche Farmacologiche "Mario Negri," Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro (Chieti), Italy.

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October 2003

Identification of domains that control the heteromeric assembly of Kir5.1/Kir4.0 potassium channels.

Am J Physiol Cell Physiol 2003 Apr 27;284(4):C910-7. Epub 2002 Nov 27.

University Laboratory of Physiology, Oxford, OX1 3PT, United Kingdom.

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April 2003