Publications by authors named "Stephanie M Jensen"

7 Publications

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Refractory Hypocalcemia Following Stomach Intestinal Pylorus-Sparing Bariatric Surgery and Thyroidectomy: Successful Management With Creation of a Proximal Roux-en-Y Gastric Bypass.

Am Surg 2021 Apr 30;87(4):576-580. Epub 2020 Oct 30.

Department of Surgery, 2331University of North Carolina, Chapel Hill, NC, USA.

Some forms of bariatric surgery make patients susceptible to calcium malabsorption, and the parathyroid hormone (PTH) axis is important for maintaining normocalcemia in these patients. Injury to the parathyroid glands due to anterior neck surgery commonly causes PTH axis disruption and can result in severe hypocalcemia in bariatric surgery patients. Herein, we present a case of a patient with a history of stomach intestinal pylorus-sparing bariatric surgery who developed refractory hypocalcemia requiring daily intravenous (IV) calcium 2 years after thyroidectomy. PTH levels were inappropriately normal during episodes of hypocalcemia, and urinary calcium level was <3.0 mg/dL following large oral doses of calcium, suggesting that both inadequate PTH response and malabsorption contributed to her severe hypocalcemia. In order to enhance calcium absorptive capacity while minimizing the risk of weight regain, she was surgically treated with a Roux-en-Y gastric bypass proximal to the prior operation. The surgery successfully improved blood calcium levels; the patient was successfully weaned from IV calcium and was able to maintain normocalcemia with oral supplements. We discuss the case in the context of available literature and provide our recommendations.
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http://dx.doi.org/10.1177/0003134820952427DOI Listing
April 2021

Does Early Low-Intensity Aerobic Exercise Hasten Recovery in Adolescents With Sport-Related Concussion?

J Sport Rehabil 2020 Feb;29(2):248-252

Clinical Scenario: Low-intensity aerobic exercise (LIAEX) below the threshold of symptom exacerbation has been shown to be superior to rest for resolving prolonged (>4 wk) symptoms following sport-related concussion (SRC), but the effects of LIAEX earlier than 4 weeks after SRC need to be elucidated. Focused Clinical Question: Does LIAEX within the first 4 weeks following SRC hasten symptom resolution? Summary of Key Findings: Two randomized controlled trials (RCT) and 1 nonrandomized trial involving adolescent athletes (10-19 y) were included. One RCT reported faster recovery time with LIAEX versus placebo stretching. Likewise, recovery time was faster with LIAEX versus rest in the nonrandomized trial, but not in the underpowered RCT, although effect sizes were similar between these studies (0.5 and 0.4, respectively). All 3 studies reported a reduction in concussion symptom severity with LIAEX; however, the magnitude of symptom reduction across the recovery timeline was greater in the LIAEX group than the rest group in the nonrandomized trial, but not the 2 RCTs. Importantly, no adverse effects or incidence of delayed recovery from LIAEX were reported in any of the studies. Clinical Bottom Line: LIAEX initiated within 10 days after SRC may facilitate a faster recovery time versus placebo stretching or rest, although additional clinical trials are strongly advised to verify this. Strength of Recommendation: Level 1b and 2b evidence suggests subsymptom exacerbation LIAEX may decrease Postconcussion Symptom Scale scores and hasten symptom resolution in adolescent athletes following SRC.
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http://dx.doi.org/10.1123/jsr.2019-0070DOI Listing
February 2020

Specific MHC-I Peptides Are Induced Using PROTACs.

Front Immunol 2018 22;9:2697. Epub 2018 Nov 22.

Discovery Chemistry and Technology, AbbVie North Chicago, IL, United States.

Peptides presented by the class-I major histocompatibility complex (MHC-I) are important targets for immunotherapy. The identification of these peptide targets greatly facilitates the generation of T-cell-based therapeutics. Herein, we report the capability of proteolysis targeting chimera (PROTAC) compounds to induce the presentation of specific MHC class-I peptides derived from endogenous cellular proteins. Using LC-MS/MS, we identified several BET-derived MHC-I peptides induced by treatment with three BET-directed PROTAC compounds. To understand our ability to tune this process, we measured the relative rate of presentation of these peptides under varying treatment conditions using label-free mass spectrometry quantification. We found that the rate of peptide presentation reflected the rate of protein degradation, indicating a direct relationship between PROTAC treatment and peptide presentation. We additionally analyzed the effect of PROTAC treatment on the entire immunopeptidome and found many new peptides that were displayed in a PROTAC-specific fashion: we determined that these identifications map to the BET pathway, as well as, potential off-target or unique-to-PROTAC pathways. This work represents the first evidence of the use of PROTAC compounds to induce the presentation of MHC-I peptides from endogenous cellular proteins, highlighting the capability of PROTAC compounds for the discovery and generation of new targets for immunotherapy.
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http://dx.doi.org/10.3389/fimmu.2018.02697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262898PMC
October 2019

Coumarin Triazabutadienes for Fluorescent Labeling of Proteins.

Chembiochem 2018 12 15;19(24):2550-2552. Epub 2018 Nov 15.

Chemistry and Biochemistry, University of Arizona, 1306 E. University Boulevard, Tucson, AZ, 85721, USA.

The use of small-molecule fluorophores to label proteins with minimal perturbation in response to an external stimulus is a powerful tool to probe chemical and biochemical environments. Herein, we describe the use of a coumarin-modified triazabutadiene that can deliver aryl diazonium ions to fluorescently label proteins by tyrosine-selective modification. The labeling can be triggered by low-pH-induced liberation of the diazonium species, thus making the fluorophore especially useful in labeling biochemical surroundings such as those found within the late endosome. Additionally, we show that a variety of coumarin triazabutadienes might also be prone to releasing their diazonium cargo after irradiation with UV light.
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http://dx.doi.org/10.1002/cbic.201800599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457986PMC
December 2018

Light-Activated Triazabutadienes for the Modification of a Viral Surface.

Chembiochem 2016 12 21;17(23):2216-2219. Epub 2016 Oct 21.

Department of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 E University Boulevard, Tucson, AZ, 85721, USA.

Chemical crosslinking is a versatile tool for the examination of biochemical interactions, in particular host-pathogen interactions. We report the critical first step toward the goal of probing these interactions by the synthesis and use of a new heterobifunctional crosslinker containing a triazabutadiene scaffold. The triazabutadiene is stable to protein conjugation and liberates a reactive aryl diazonium species upon irradiation with 350 nm light. We highlight the use of this technology by modifying the surface of several proteins, including the dengue virus envelope protein.
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http://dx.doi.org/10.1002/cbic.201600508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170875PMC
December 2016

A gradient-free method for the purification of infective dengue virus for protein-level investigations.

J Virol Methods 2016 09 2;235:125-130. Epub 2016 Jun 2.

The Department of Chemistry and Biochemistry, The University of Arizona, 1306 E. University Blvd., Tucson, AZ 85721, USA. Electronic address:

Dengue virus (DENV) is a mosquito-transmitted flavivirus that infects approximately 100 million people annually. Multi-day protocols for purification of DENV reduce the infective titer due to viral sensitivity to both temperature and pH. Herein we describe a 5-h protocol for the purification of all DENV serotypes, utilizing traditional gradient-free ultracentrifugation followed by selective virion precipitation. This protocol allows for the separation of DENV from contaminating proteins - including intact C6/36 densovirus, for the production of infective virus at high concentration for protein-level analysis.
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http://dx.doi.org/10.1016/j.jviromet.2016.05.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992633PMC
September 2016

A traceless Staudinger reagent to deliver diazirines.

Org Lett 2013 Oct 23;15(19):5060-3. Epub 2013 Sep 23.

University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.

A triarylphosphine reagent that reacts with organic azides to install amide-linked diazirines is reported. This traceless Staudinger reagent reacts with complex organic azides to yield amide-linked diazirines, thus expanding the scope of the utility of both azide and diazirine chemistry.
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http://dx.doi.org/10.1021/ol402404nDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857746PMC
October 2013