Publications by authors named "Stefano Vullo"

4 Publications

  • Page 1 of 1

New Real-Time PCRs to Differentiate spp. and .

Molecules 2020 Sep 27;25(19). Epub 2020 Sep 27.

Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90100 Palermo, Italy.

species are an important cause of emerging infectious diseases in people and animals, and rickettsiosis is one of the oldest known vector-borne diseases. Laboratory diagnosis of is complex and time-consuming. This study was aimed at developing two quantitative real-time PCRs targeting and genes for the detection, respectively, of spp. and DNA. Primers were designed following an analysis of gene sequences. The assays were optimized using SYBR Green and TaqMan methods and tested for sensitivity and specificity. This study allowed the development of powerful diagnostic methods, able to detect and quantify spp. DNA and differentiate species.
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http://dx.doi.org/10.3390/molecules25194431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582818PMC
September 2020

Innate Immune Response to Tick-Borne Pathogens: Cellular and Molecular Mechanisms Induced in the Hosts.

Int J Mol Sci 2020 Jul 30;21(15). Epub 2020 Jul 30.

Central Laboratory of Advanced Diagnostic and Biological Research (CLADIBIOR), BIND, University Hospital "Paolo Giaccone", Università degli studi di Palermo, Via del Vespro 129, 90100 Palermo, Italy.

Many pathogens are transmitted by tick bites, including spp., spp., spp., and sensu stricto species. These pathogens cause infectious diseases both in animals and humans. Different types of immune effector mechanisms could be induced in hosts by these microorganisms, triggered either directly by pathogen-derived antigens or indirectly by molecules released by host cells binding to these antigens. The components of innate immunity, such as natural killer cells, complement proteins, macrophages, dendritic cells and tumor necrosis factor alpha, cause a rapid and intense protection for the acute phase of infectious diseases. Moreover, the onset of a pro-inflammatory state occurs upon the activation of the inflammasome, a protein scaffold with a key-role in host defense mechanism, regulating the action of caspase-1 and the maturation of interleukin-1β and IL-18 into bioactive molecules. During the infection caused by different microbial agents, very similar profiles of the human innate immune response are observed including secretion of IL-1α, IL-8, and IFN-α, and suppression of superoxide dismutase, IL-1Ra and IL-17A release. Innate immunity is activated immediately after the infection and inflammasome-mediated changes in the pro-inflammatory cytokines at systemic and intracellular levels can be detected as early as on days 2-5 after tick bite. The ongoing research field of "inflammasome biology" focuses on the interactions among molecules and cells of innate immune response that could be responsible for triggering a protective adaptive immunity. The knowledge of the innate immunity mechanisms, as well as the new targets of investigation arising by bioinformatics analysis, could lead to the development of new methods of emergency diagnosis and prevention of tick-borne infections.
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http://dx.doi.org/10.3390/ijms21155437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432002PMC
July 2020

Tetracycline Residues in Bovine Muscle and Liver Samples from Sicily (Southern Italy) by LC-MS/MS Method: A Six-Year Study.

Molecules 2019 Feb 15;24(4). Epub 2019 Feb 15.

Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", via Gino Marinuzzi 3, 90129 Palermo, Italy.

We examined a total of 369 bovine liver and muscle samples for the detection of oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), and doxycycline (DOX) residues by implementation and validation of a LC-MS/MS method. The method showed good recovery values between 86% and 92% at three levels of concentrations. The linearity tests revealed r² > 0.996 for all the tetracyclines examined. Furthermore, the Youden test revealed that the method was robust. Only 14.4% of the samples showed OTC and TC residues in a concentration range of 10.4⁻40.2 µg kg. No CTC and DOX residues were found in all the samples analyzed. Liver samples showed the highest average values (31.5 ± 20.6 and 21.8 ± 18.9 for OTC and TC, respectively). The results showed a low incidence of TCs in all the samples examined, in comparison with other studies reported in the literature. A significant decrease in TC residues frequency was found from 2013 ( < 0.05). This work reports for the first time epidemiological data on the presence of TC residues in liver and muscle samples of cattle farmed in Sicily (Southern Italy). The very low incidence of TC residues indicates a continuous improvement in farming techniques in Southern Italy, which is essential to ensure consumers' protection.
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http://dx.doi.org/10.3390/molecules24040695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413177PMC
February 2019

Spatial distribution of brucellosis in sheep and goats in Sicily from 2001 to 2005.

Vet Ital 2007 Jul-Sep;43(3):541-8

Area Sorveglianza Epidemiologica - Istituto Zooprofilattico Sperimentale della Sicilia A. Mirri, Via Gino Marinuzzi 3, 90129 Palermo, Italy.

Spatial analysis is making an increasingly important contribution to surveillance measures due to its ability to enable immediate visualization of information on the phenomenon studied. The authors describe the spatial distribution of prevalence and incidence of brucellosis in small ruminants in Sicily between 2001 and 2005. The study was conducted by integrating geographic information systems (GIS) technology (MapInfo Professional 7.0) with SaTScan software to perform an epidemiological analysis of the municipalities and to locate problem areas. A comparison between the thematic maps produced for brucellosis in small ruminants on the basis of prevalence and incidence data for each individual year has shown that in terms of prevalence, the area identified as the secondary cluster in 2001 became the primary cluster from 2002 onwards whereas, in terms of incidence, the distribution of the clusters was irregular throughout the entire region during the years studied.
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May 2010
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