Publications by authors named "Soujanya Kuntam"

4 Publications

  • Page 1 of 1

Thin cell layer cultures of Chlamydomonas reinhardtii L159I-N230Y, pgrl1 and pgr5 mutants perform enhanced hydrogen production at sunlight intensity.

Bioresour Technol 2021 Aug 27;333:125217. Epub 2021 Apr 27.

Institute of Plant Biology, Biological Research Centre, Szeged, Temesvári krt. 62, H-6726 Szeged, Hungary. Electronic address:

Photobiological hydrogen (H) production is a promising renewable energy source. HydA hydrogenases of green algae are efficient but O-sensitive and compete for electrons with CO-fixation. Recently, we established a photoautotrophic H production system based on anaerobic induction, where the Calvin-Benson cycle is inactive and O scavenged by an absorbent. Here, we employed thin layer cultures, resulting in a three-fold increase in H production relative to bulk CC-124 cultures (50 µg chlorophyll/ml, 350 µmol photons m s). Productivity was maintained when increasing the light intensity to 1000 µmol photons ms and the cell density to 150 µg chlorophyll/ml. Remarkably, the L159I-N230Y photosystem II mutant and the pgrl1 photosystem I cyclic electron transport mutant produced 50% more H than CC-124, while the pgr5 mutant generated 250% more (1.2 ml H/ml culture in six days). The photosynthetic apparatus of the pgr5 mutant and its in vitro HydA activity remained remarkably stable.
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http://dx.doi.org/10.1016/j.biortech.2021.125217DOI Listing
August 2021

Characterization of a new class of blue-fluorescent lipid droplet markers for live-cell imaging in plants.

Plant Cell Rep 2015 Apr 21;34(4):655-65. Epub 2015 Jan 21.

Cellular Imaging Laboratory, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary.

Key Message: The present work demonstrates the use and advantages of novel, live cell permeable, lipid droplet localizing, non toxic, blue fluorochromes for use in live plant cells. Lipid droplets (LDs) are ubiquitous components of both animal and plant cells. They consist of a core of neutral lipids surrounded by a monolayer of phospholipids, glycolipids and/or sterols with embedded amphipathic proteins. Although initially considered to be simple energy depots, they have recently emerged as organelles that serve important regulatory functions. Here we report three new fluorochromes as markers for LDs in plants. These bright blue fluorochromes with their unique spectral properties can easily be combined with other green and red fluorescent reporters for multicolor fluorescence imaging. The fluorochromes are non-toxic and photo-stable. All in all, they represent a reliable tool to use, for the investigation of dynamic LD biology within living plant cells using fluorescence microscopy.
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http://dx.doi.org/10.1007/s00299-015-1738-4DOI Listing
April 2015

Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery.

Molecules 2013 Aug 20;18(8):9999-10013. Epub 2013 Aug 20.

Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged H-6726, Hungary.

Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1-10 micromolar range, they were non-toxic even during long-term incubations.
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http://dx.doi.org/10.3390/molecules18089999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270374PMC
August 2013