Publications by authors named "Sooyeon Park"

254 Publications

sp. nov., isolated from a sand dune.

Authors:
Sooyeon Park Seo Yeon Lee Jung-Sook Lee Wonyong Kim Jung-Hoon Yoon

Int J Syst Evol Microbiol 2021 Feb 22. Epub 2021 Feb 22.

Department of Food Science and Biotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterial strain, designated BSSL-BM3, was isolated from sand collected from a dune near the Yellow Sea, Republic of Korea, and subjected to a polyphasic taxonomic study. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BSSL-BM3 fell within the clade comprising the type strains of species. Strain BSSL-BM3 exhibited 16S rRNA gene sequence similarity values of 98.0-99.0 % to the type strains of , , , and and of 94.2-96.7 % to the type strains of the other species. The averagenucleotide identity and digitalDNA-DNA hybridization values between strain BSSL-BM3 and the type strains of , , , and were 82.2-88.8 % and 25.0-36.5 %, respectively. The DNA G+C content of strain BSSL-BM3 from genomic sequence data was 38.75 mol%. Strain BSSL-BM3 contained MK-6 as the predominant menaquinone and iso-C 3-OH, summed feature 3 (C7 and/or C6) and iso-C G as the major fatty acids. The major polar lipids of strain BSSL-BM3 were phosphatidylethanolamine and two unidentified lipids. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain BSSL-BM3 is separated from recognized species. On the basis of the data presented here, strain BSSL-BM3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BSSL-BM3 (=KACC 21632=NBRC 114502).
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http://dx.doi.org/10.1099/ijsem.0.004709DOI Listing
February 2021

sp. nov., a gammaproteobacterium isolated from seawater.

Authors:
Young-Ok Kim In-Suk Park Sooyeon Park Jung-Hoon Yoon

Int J Syst Evol Microbiol 2020 Dec;70(12):6396-6401

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 16419, South Korea.

A Gram-stain-negative, aerobic, non-spore-forming, non-motile and ovoid or rod-shaped bacterial strain, MYP5, was isolated from seawater in Jeju island of South Korea. MYP5 grew optimally at 30-35 °C and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that MYP5 fell within the clade enclosed by the type strains of species of the genus , clustering with the type strains of and . MYP5 exhibited the highest 16S rRNA gene sequence similarity value (98.0 %) to the type strain of and similarities of 95.1-97.9 % to the type strains of the other species of the genus . ANI and dDDH values of genomic sequences between MYP5 and the type strains of 22 species of the genus were 66.8-70.5 % and 18.6-27.5 %, respectively. The DNA G+C content of MYP5, determined from the genome sequence, was 46.1 %. MYP5 contained Q-8 as the predominant ubiquinone and C ω7, summed feature 3 (C ω7 and/or C ω6), C and 10-methyl C as the major fatty acids. The major polar lipids of MYP5 were phosphatidylethanolamine and phosphatidylglycerol. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that MYP5 is separated from species of the genus . On the basis of the data presented, MYP5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MYP5 (=KCTC 82144=NBRC 114354).
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http://dx.doi.org/10.1099/ijsem.0.004545DOI Listing
December 2020

sp. nov., isolated from a tidal flat.

Authors:
Sooyeon Park Seo Yeon Lee Wonyong Kim Jung-Hoon Yoon

Int J Syst Evol Microbiol 2020 Dec;70(12):6301-6306

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative, aerobic, non-spore-forming, motile by single polar flagellum and ovoid or rod-shaped bacterial strain, designated JBTF-M23, was isolated from tidal flat sediment collected from the Yellow Sea, Republic of Korea. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M23 fell within the clade comprising the type strains of species, clustering with the type strains of and . Strain JBTF-M23 exhibited the highest 16S rRNA gene sequence similarity value (98.6 %) to the type strain of and sequence similarities of 98.3 and 97.7 % to the type strains of and respectively. The DNA G+C content of strain JBTF-M23 from genomic sequence data was 41.98 %. The ANI and dDDH values between strain JBTF-M23 and the type strains of , and were 71.3-76.6 and 19.4-19.9 %, respectively. Strain JBTF-M23 contained Q-8 as the predominant ubiquinone and C7 and/or C6, C and C7 as the major fatty acids. The major polar lipids of strain JBTF-M23 were phosphatidylethanolamine and one unidentified aminolipid. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain JBTF-M23 is separated from recognized species. On the basis of the data presented, strain JBTF-M23is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JBTF-M23(=KACC 19900=NBRC 113647).
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http://dx.doi.org/10.1099/ijsem.0.004532DOI Listing
December 2020

Ping-Pong Free Advanced and Energy Efficient Sensor Relocation for IoT-Sensory Network.

Authors:
Moonseong Kim Sooyeon Park Woochan Lee

Sensors (Basel) 2020 Oct 2;20(19). Epub 2020 Oct 2.

Department of Electrical Engineering, Incheon National University, Incheon 22012, Korea.

With the growing interest in big data technology, mobile IoT devices play an essential role in data collection. Generally, IoT sensor nodes are randomly distributed to areas where data cannot be easily collected. Subsequently, when data collection is impossible (i.e., sensing holes occurrence situation) due to improper placement of sensors or energy exhaustion of sensors, the sensors should be relocated. The cluster header in the sensing hole sends requests to neighboring cluster headers for the sensors to be relocated. However, it can be possible that sensors in the specific cluster zones near the sensing hole are continuously requested to move. With this knowledge, there can be a ping-pong problem, where the cluster headers in the neighboring sensing holes repeatedly request the movement of the sensors in the counterpart sensing hole. In this paper, we first proposed the near-uniform selection and movement scheme of the sensors to be relocated. By this scheme, the energy consumption of the sensors can be equalized, and the sensing capability can be extended. Thus the network lifetime can be extended. Next, the proposed relocation protocol resolves a ping-pong problem using queues with request scheduling. Another crucial contribution of this paper is that performance was analyzed using the fully-customed OMNeT++ simulator to reflect actual environmental conditions, not under over-simplified artificial network conditions. The proposed relocation protocol demonstrates a uniform and energy-efficient movement with ping-pong free capability.
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http://dx.doi.org/10.3390/s20195654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583995PMC
October 2020

Characterization of a novel carboxylesterase belonging to family VIII hydrolyzing β-lactam antibiotics from a compost metagenomic library.

Authors:
Ji-Min Park Sung-Min Won Chul-Hyung Kang Sooyeon Park Jung-Hoon Yoon

Int J Biol Macromol 2020 Dec 15;164:4650-4661. Epub 2020 Sep 15.

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea. Electronic address:

A novel esterase, EstCS3, was isolated from a metagenomic library constructed from a compost. The EstCS3, which consists of 409 amino acids with an anticipated molecular mass of 44 kDa, showed high amino acid sequence identities to predicted esterases, serine hydrolases and β-lactamases from uncultured and cultured bacteria. Phylogenetic analysis suggested that EstCS3 belongs to family VIII of lipolytic enzymes. EstCS3 had catalytic Ser78 residue in the consensus tetrapeptide motif SXXK, which is characteristic of family VIII esterases. Two conserved YXX and W(H or K)XG motifs in an oxyanion hole of family VIII esterases were also present in EstCS3. EstCS3 demonstrated the highest activity toward p-nitrophenyl butyrate (C4) and was stable up to 70 °C with optimal activity at 55 °C. EstCS3 had optimal activity at pH 8 and maintained its stability within pH range of 7-10. EstCS3 had over 70% activity in the presence of 20% (v/v) methanol and DMSO and hydrolyzed sterically hindered tertiary alcohol esters of t-butyl acetate and linalyl acetate. EstCS3 hydrolyzed ampicillin, cephalothin and cefepime. The properties of EstCS3, including moderate thermostability, stability against organic solvents and activity toward esters of tertiary alcohols, indicated that it has the potential to be used in industrial applications.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.09.070DOI Listing
December 2020

Description of Shewanella salipaludis sp. nov., isolated from a salt marsh.

Authors:
Sooyeon Park Siyu Chen Jung-Sook Lee Wonyong Kim Jung-Hoon Yoon

FEMS Microbiol Lett 2020 Aug;367(15)

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, 16419, Republic of Korea.

A Gram-stain-negative and flagellated bacterial strain, SHSM-M6T, was isolated from salt marsh from Yellow Sea, Republic of Korea. Neighbor-joining phylogenetic tree of 16S rRNA gene sequences showed that strain SHSM-M6T belongs to the genus Shewanella. 16S rRNA gene sequence similarity values between strain SHSM-M6T and the type strains of Shewanella species were <98.0%. The average nucleotide identity and DNA-DNA hybridization values between genomic sequences of strain SHSM-M6T and the type strains of Shewanella species were <73.3 and 20.7%, respectively. Strain SHSM-M6T contained MK-6 as predominant menaquinone and Q-7 and Q-8 as the predominant ubiquinones. The novel strain contained C16:1ω7c and/or C16:1ω6c, iso-C15:0 and C16:0 as major fatty acids. Major polar lipids of strain SHSM-M6T were phosphatidylethanolamine, phosphatidylglycerol, one unidentified lipid, one unidentified aminolipid and one unidentified phospholipid. Differential phenotypic properties of strain SHSM-M6T, together with its phylogenetic and genetic distinctiveness, revealed that strain SHSM-M6T is separated from recognized Shewanella species. On the basis of the data presented, strain SHSM-M6T is considered to represent a novel species of the genus Shewanella, for which the name Shewanella salipaludis sp. nov. is proposed. The type strain is SHSM-M6T (=KACC 19901T = NBRC 113646T).
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http://dx.doi.org/10.1093/femsle/fnaa121DOI Listing
August 2020

Noncanonical Head-to-Head Hairpin DNA Dimerization Is Essential for the Synthesis of Orange Emissive Silver Nanoclusters.

Authors:
Pratik Shah Riddhi Nagda Il Lae Jung Yong Joo Bhang Sang-Woo Jeon Chang Seop Lee Changwoo Do Keonwook Nam Young Min Kim Sooyeon Park Young Hoon Roh Peter Waaben Thulstrup Morten Jannik Bjerrum Tae-Hwan Kim Seong Wook Yang

ACS Nano 2020 07 22;14(7):8697-8706. Epub 2020 Jun 22.

Department of Systems Biology, Institute of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.

DNA secondary structures, such as dimers and hairpins, are important for the synthesis of DNA template-embedded silver nanoclusters (DNA/AgNCs). However, the arrangement of AgNCs within a given DNA template and how the AgNC influences the secondary structure of the DNA template are still unclear. Here, we introduce a noncanonical head-to-head hairpin DNA nanostructure that is driven by orange-emissive AgNCs. Through detailed in-gel analysis, sugar backbone switching, inductively coupled plasma mass spectrometry, small-angle X-ray scattering, and small angle neutron scattering, we show that the orange-emissive AgNCs mediate cytosine-Ag-cytosine bridging between two six-cytosine loop (6C-loop) hairpin DNA templates. Unlike green, red, or far-red emissive AgNCs, which are embedded inside a hairpin and duplex DNA template, the orange-emissive AgNCs are localized on the interface between the two 6C-loop hairpin DNA templates, thereby linking them. Moreover, we found that deoxyribose in the backbone of the 6C-loop at the third and fourth cytosines is crucial for the formation of the orange-emissive AgNCs and the head-to-head hairpin DNA structure. Taken together, we suggest that the specific wavelength of AgNCs fluorescence is determined by the mutual interaction between the secondary or tertiary structures of DNA- and AgNC-mediated intermolecular DNA cross-linking.
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http://dx.doi.org/10.1021/acsnano.0c03095DOI Listing
July 2020

sp. nov., isolated from a tidal flat.

Authors:
Sooyeon Park In Kyu Kim Wonyong Kim Jung-Hoon Yoon

Int J Syst Evol Microbiol 2020 Jun;70(6):3872-3877

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative, aerobic, non-spore-forming, motile by single polar flagellum and ovoid or rod-shaped bacterial strain, designated JBTF-M18, was isolated from tidal-flat sediment collected from the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M18 fell within the clade comprising the type strains of species. Strain JBTF-M18 exhibited 16S rRNA gene sequence similarity values of 97.1-98.8 % to the type strains of , , and and of less than 96.9 % to the type strains of the other species. The average nucleotide identity and digital DNA-DNA hybridization values between strain JBTF-M18 and the type strains of and were 72.0 and 89.5% and 18.9 and 38.1 %, respectively. DNA-DNA relatedness values between strain JBTF-M18 and the type strains of and were 14 and 19 %, respectively. The DNA G+C content of strain JBTF-M18 from genomic sequence data was 52.9 %. Strain JBTF-M18contained MK-6 as the predominant menaquinone and Q-7 and Q-8 as the predominant ubiquinones. It had iso-C, summed feature 3 (Cω and/or Cω) and C as the major fatty acids. The major polar lipids of strain JBTF-M18 were phosphatidylethanolamine and phosphatidylglycerol. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain JBTF-M18 is separated from recognized species. On the basis of the data presented, strain JBTF-M18 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JBTF-M18 (=KACC 19869=NBRC 113583).
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http://dx.doi.org/10.1099/ijsem.0.004252DOI Listing
June 2020

Description of Sulfitobacter sediminilitoris sp. nov., isolated from a tidal flat.

Authors:
Sooyeon Park Siyu Chen Jung-Sook Lee Wonyong Kim Jung-Hoon Yoon

FEMS Microbiol Lett 2020 05;367(9)

Department of Food Science and Biotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative bacterial strain, JBTF-M27T, was isolated from a tidal flat from Yellow Sea, Republic of Korea. Neighbor-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M27T fell within the clade comprising the type strains of Sulfitobacter species. Strain JBTF-M27T exhibited the highest 16S rRNA gene sequence similarity (98.8%) to the type strain of S. porphyrae. Genomic ANI and dDDH values of strain JBTF-M27T between the type strains of Sulfitobacter species were less than 76.1 and 19.2%, respectively. Mean DNA-DNA relatedness value between strain JBTF-M27T and the type strain of S. porphyrae was 21%. DNA G + C content of strain JBTF-M27T from genome sequence was 57.8% (genomic analysis). Strain JBTF-M27T contained Q-10 as the predominant ubiquinone and C18:1ω7c as the major fatty acid. The major polar lipids of strain JBTF-M27T were phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain JBTF-M27T is separated from recognized Sulfitobacter species. On the basis of the data presented, strain JBTF-M27T ( = KACC 21648T = NBRC 114356T) is considered to represent a novel species of the genus Sulfitobacter, for which the name Sulfitobacter sediminilitoris sp. nov. is proposed.
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http://dx.doi.org/10.1093/femsle/fnaa074DOI Listing
May 2020

sp. nov., isolated from a tidal flat.

Authors:
Sooyeon Park Siyu Chen Jung-Hoon Yoon

Int J Syst Evol Microbiol 2020 Mar;70(3):1470-1477

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A Gram-staining-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated as JBTF-M21, was isolated from a tidal flat sediment on the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences indicated that JBTF-M21 fell within the clade comprising the type strains of species of the genus . JBTF-M21 exhibited 16S rRNA gene sequence similarities of 97.0-98.4 % to the type strains of , , , , , and and 93.7-96.6 % to the type strains of the other species of the genus . The ANI and dDDH values between JBTF-M21 and the type strains of , , and were 70.83-72.93 % and 18.0-18.8 %, respectively. Mean DNA-DNA relatedness values between JBTF-M21 and the type strains of , and were 12-24 %. The DNA G+C content of JBTF-M21 was 57.0 mol%. JBTF-M21 contained Q-10 as the predominant ubiquinone and Cω7 and Cω6 as the major fatty acids. The major polar lipids ofJBTF-M21 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. Distinguishing phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that JBTF-M21 is separated from species of the genus with validly published names. On the basis of the data presented, strain JBTF-M21 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JBTF-M21 (=KACC 19864=NBRC 113584).
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http://dx.doi.org/10.1099/ijsem.0.003824DOI Listing
March 2020

sp. nov., isolated from a marine sand.

Authors:
Sooyeon Park In Kyu Kim Wonyong Kim Jung-Hoon Yoon

Int J Syst Evol Microbiol 2020 Feb;70(2):909-914

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative, aerobic, non-spore-forming, motile by gliding and rod-shaped bacterial strain, designated HSMS-1, was isolated from a marine sand collected from the Yellow Sea, Republic of Korea, and identified by a polyphasic taxonomic approach. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that HSMS-1 fell within the clade comprising the type strains of species of the genus . HSMS-1 exhibited 16S rRNA gene sequence similarity values of 99.0 and 98.7 % to the type strains of and and of 93.3-98.5 % to the type strains of the other species of the genus . The ANI and dDDH values between HSMS-1 and the type strains of , , , , and were 72.6-79.3 % and 17.4-22.2 %, respectively. Mean DNA-DNA relatedness value between HSMS-1 and the type strain of was 18 %. HSMS-1 contained MK-6 as the predominant menaquinone and iso-C, anteiso-C, iso-C 3-OH and iso-C as the major fatty acids. The major polar lipid of HSMS-1 was phosphatidylethanolamine. The DNA G+C content of HSMS-1 from genomic sequence data was 39.2 %. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that HSMS-1 is separated from recognized species of the genus . On the basis of the data presented, strain HSMS-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HSMS-1(=KACC 19899=NBRC 113648).
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http://dx.doi.org/10.1099/ijsem.0.003845DOI Listing
February 2020

sp. nov., isolated from marine sand.

Authors:
Sooyeon Park Sung Min Won Jung-Hoon Yoon

Int J Syst Evol Microbiol 2020 Jan;70(1):246-250

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 16419, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile and ovoid- or rod-shaped bacterial strain, designated HSMS-39, was isolated from marine sand sampled at Hongsung, Republic of Korea. Strain HSMS-39 grew optimally at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain HSMS-39 fell within the clade comprising the type strains of species, clustering with the type strain of showing 16S rRNA gene sequence similarity of 99.1 %. It exhibited 16S rRNA gene sequence similarities of less than 95.5 % to the type strains of the other species. Strain HSMS-39 contained MK-7 as the predominant menaquinone and iso-Cω9, iso-C and summed feature 3 (Cω7 and/or Cω6) as the major fatty acids. The major polar lipids of strain HSMS-39 were phosphatidylethanolamine and one unidentified phospholipid. The DNA G+C content of strain HSMS-39 was 60.0 mol%. The mean DNA-DNA relatedness value between strain HSMS-39 and the type strain of was 15 %. The average nucleotide identity value between strain HSMS-39and the type strain of was 81.87 %. The phylogenetic and genetic data and differential phenotypic properties indicated that strain HSMS-39 is separated from other recognized species of the genus . On the basis of the polyphasic data, strain HSMS-39 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HSMS-39 (=KACC 19866=NBRC 113585).
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http://dx.doi.org/10.1099/ijsem.0.003747DOI Listing
January 2020

Description of Polaribacter aestuariivivens sp. nov., isolated from a tidal flat.

Authors:
Sooyeon Park Ji-Min Park Jung-Sook Lee Jung-Hoon Yoon

FEMS Microbiol Lett 2019 08;366(15)

Department of Food Science and Biotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 16419, Republic of Korea.

A bacterial strain, DBTF-3T, was isolated from a tidal flat of Republic of Korea. Phylogenetic trees of 16S rRNA gene sequences showed that strain DBTF-3T belonged to the genus Polaribacter. Strain DBTF-3T exhibited 16S rRNA gene sequence similarities of 97.1-98.1% to type strains of P. dokdonensis, P. haliotis, P. marinaquae, P. insulae, P. vadi, P. glomeratus, P. irgensii and P. reichenbachii, and 94.0-96.9% to those of the other Polaribacter species. DNA-DNA relatedness values of strain DBTF-3T with type strains of P. marinaquae and P. insulae were 14-19%. Average nucleotide identity and digital DNA-DNA hybridization values between strain DBTF-3T and type strains of six other Polaribacter species were 76.5-83.5% and 20.9-27.1%, respectively. Strain DBTF-3T contained MK-6 as the predominant menaquinone, and iso-C15:0, summed feature 3, iso-C15:1 G and iso-C15:0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain DBTF-3T is separated from Polaribacter species. On the basis of the data presented, strain DBTF-3T (= KACC 19612T = NBRC 113191T) represents a novel species of the genus Polaribacter, for which the name Polaribacter aestuariivivens sp. nov. is proposed.
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http://dx.doi.org/10.1093/femsle/fnz185DOI Listing
August 2019

sp. nov., isolated from marine sand.

Authors:
Sooyeon Park In Kyu Kim Jung-Sook Lee Jung-Hoon Yoon

Int J Syst Evol Microbiol 2019 Oct;69(10):3230-3236

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, HSMS-29, was isolated from a marine sand sample collected from the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain HSMS-29 fell within the clade comprising the type strains of species. Strain HSMS-29 exhibited 16S rRNA gene sequence similarities of 97.2-98.4 % to the type strains of , , , , , , and and 96.3-96.9 % to the type strains of the other species. The genomic ANI values of strain HSMS-29 with the type strains of , , , , , and were 72.66-74.99 %. The DNA-DNA relatedness value between strain HSMS-29 and the type strain of was 17 %. Strain HSMS-29 contained Q-10 as the predominant ubiquinone and C7 as the major fatty acid. The major polar lipids of strain HSMS-29 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain HSMS-29 was 65.0 mol% (HPLC) or 64.4 % (genome analysis). Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain HSMS-29 is separated from recognized species. On the basis of the data presented here, strain HSMS-29 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HSMS-29 (=KACC 19870=NBRC 113549).
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http://dx.doi.org/10.1099/ijsem.0.003614DOI Listing
October 2019

gen. nov., sp. nov., isolated from seawater.

Authors:
Young-Ok Kim In-Suk Park Sooyeon Park Hakju Kim Bo-Hye Nam Dong-Gyun Kim Sung-Min Won Jung-Hoon Yoon

Int J Syst Evol Microbiol 2019 Oct;69(10):3109-3115

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 16419, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated MYP11, was isolated from seawater around Jeju island, Republic of Korea and identified by polyphasic taxonomic study. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain MYP11 joined the cluster comprising the type strains of , and , showing 16S rRNA gene sequence similarities of 96.3-96.8 %. Strain MYP11 exhibited 16S rRNA gene sequence similarity values of 94.2-94.9 % to the type strains of other species. In the upgma dendrogram based on the average nucleotide identity values of genomic sequences, strain MYP11 formed an evolutionary lineage independent of those of species and other taxa. Strain MYP11 contained Q-10 as the predominant ubiquinone and C7 and cyclo C8 as the major fatty acids. The major polar lipids of strain MYP11 were phosphatidylcholine, phosphatidylglycerol, two unidentified lipids and one unidentified aminolipid. The DNA G+C content of strain MYP11 was 63.1 or 61.5 mol%. The differences in the fatty acid and polar lipid profiles and DNA G+C contents made it reasonable to distinguish strain MYP11 from the type strains of , and and those of other species. On the basis of the polyphasic data presented here, strain MYP11 is considered to constitute a new genus and species within the class , for which the name gen. nov., sp. nov. is proposed. The type strain is MYP11 (=KCTC 62899=NBRC 113544).
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http://dx.doi.org/10.1099/ijsem.0.003596DOI Listing
October 2019

sp. nov., isolated from seawater.

Authors:
Sooyeon Park Ji-Min Park Jung-Hoon Yoon

Int J Syst Evol Microbiol 2019 Oct;69(10):3062-3067

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.

A Gram-stain-negative, aerobic, motile and rod-shaped bacterial strain, designated OISW-25, was isolated from seawater in Republic of Korea. Strain OISW-25 grew optimally at 25 °C and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OISW-25 fell within the clade comprising the type strains of species. Strain OISW-25 exhibited 16S rRNA gene sequence similarity values of 97.5, 97.2 and 97.1 % to the type strains of , and , respectively, and of 93.6-96.6 % to the type strains of the other species. The average nucleotide identity values between strain OISW-25 and ATCC BAA-637 and two non-type strains of were 78.16-79.35 % and DNA-DNA relatedness value of strain OISW-25 with the type strain of . was 17 %. The DNA G+C content of strain OISW-25 was 39.2 mol% (HPLC) or 38.7 mol% (genome data). Strain OISW-25 contained Q-8 as the predominant ubiquinone and summed feature 3 (C7 and/or C6) and C as the major fatty acids. The major polar lipids of strain OISW-25 were phosphatidylethanolamine and phosphatidylglycerol. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain OISW-25 is distinct from species. On the basis of the data presented, strain OISW-25 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is OISW-25 (=KCTC 62426=NBRC 113187).
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http://dx.doi.org/10.1099/ijsem.0.003590DOI Listing
October 2019

gen. nov., sp. nov., isolated from a tidal flat.

Authors:
Sooyeon Park Sung Min Won Jung-Hoon Yoon

Int J Syst Evol Microbiol 2019 Oct 9;69(10):3049-3055. Epub 2019 Jul 9.

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative, strictly aerobic, non-motile and ovoid- or rod-shaped bacterial strain, JBTF-M29, was isolated from tidal flat sediment sampled from the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain JBTF-M29 clustered with the type strains of species and this cluster joined the clade comprising and species. Strain JBTF-M29 exhibited 16S rRNA gene sequence similarity values of 96.2-96.6 % to the type strains of three species, of 96.4 % to the type strain of and of 93.9-94.7 % to the type strains of three species. In the UPGMA dendrogram based on the ANI values of genomic sequences, strain JBTF-M29 formed an evolutionary lineage independent of the genera and and some other taxa. Strain JBTF-M29 contained Q-10 as the predominant ubiquinone and C7 and C as the major fatty acids. The major polar lipids of strain JBTF-M29 were phosphatidylglycerol and one unidentified aminolipid. The DNA G+C content of strain JBTF-M29 was 64.5 mol%. The differences in fatty acid and polar lipid profiles and other differential phenotypic properties including nitrate reduction and casein hydrolysis made it reasonable to distinguish strain JBTF-M29 from the genera , and . Therefore, on the basis of the polyphasic taxonomic data presented here, strain JBTF-M29 constitutes a new genus and species within the class , for which the name gen. nov., sp. nov. is proposed. The type strain is JBTF-M29 (=KACC 19867=NBRC 113548).
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http://dx.doi.org/10.1099/ijsem.0.003588DOI Listing
October 2019

sp. nov., isolated from an intestine of Nile tilapia ().

Authors:
Young-Ok Kim Sooyeon Park In-Suk Park Bo-Hye Nam Dong-Gyun Kim Jung-Hoon Yoon

Int J Syst Evol Microbiol 2019 Sep;69(9):2781-2786

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 16419, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, MRS2, was isolated from an intestine of Nile tilapia () collected from the Republic of Korea. Strain MRS2 grew optimally at 30 °C and in the presence of 0-2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain MRS2 clustered with the type strains of species. It exhibited the highest 16S rRNA gene sequence similarity (98.5 %) to the type strain of and sequence similarities of 97.4-97.6 % to the type strains of two other species. Strain MRS2 contained MK-6 as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6), iso-C 3-OH and iso-C as the major fatty acids. The major polar lipids of strain MRS2 were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C contents of strain MRS2 were 32.2 mol% or 30.65 mol%. Strain MRS2 exhibited DNA-DNA relatedness values of 12-20 % to the type strains of , and . The average nucleotide identity values between strain MRS2 and five strains of and were 84.8-91.0 %. The phylogenetic, genetic and differential phenotypic properties indicated that strain MRS2 is separated from species. On the basis of the data presented here, strain MRS2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MRS2 (=KCTC 62904=NBRC 113550).
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http://dx.doi.org/10.1099/ijsem.0.003558DOI Listing
September 2019

sp. nov., isolated from a salt marsh.

Authors:
Sooyeon Park Ji-Min Park Jung-Hoon Yoon

Int J Syst Evol Microbiol 2019 Sep;69(9):2750-2754

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 16419, South Korea.

A Gram-stain-negative, aerobic, non-motile and rod-shaped or filamentous bacterial strain, designated SHSM-M15, was isolated from a salt marsh at Siheung in Republic of Korea and identified by polyphasic taxonomic study. Strain SHSM-M15 grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain SHSM-M15 clusters with the type strain of , showing 16S rRNA gene sequence similarity of 97.8 %. Strain SHSM-M15 had 16S rRNA gene sequence similarities of less than 93.7 % with the type strains of other recognised species. Strain SHSM-M15 contained MK-7 as the predominant menaquinone and iso-C, iso-C 3-OH and anteiso-C as the major fatty acids. The major polar lipid detected in strain SHSM-M15 was phosphatidylethanolamine. The DNA G+C content of strain SHSM-M15 from genomic sequence was 36.6 %. Mean DNA-DNA relatedness value between strain SHSM-M15 and the type strain of was 18 % and the average nucleotide identity value between strain SHSM-M15 and the type strain of was 87.98 %. The phylogenetic and genetic data and differential phenotypic properties indicated that strain SHSM-M15 is separated from . On the basis of the polyphasic data presented, strain SHSM-M15 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SHSM-M15 (=KACC 19862=NBRC 113749).
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http://dx.doi.org/10.1099/ijsem.0.003553DOI Listing
September 2019

sp. nov., isolated from isolated from estuary water.

Authors:
Sooyeon Park Sung Min Won Jung-Hoon Yoon

Int J Syst Evol Microbiol 2019 Sep;69(9):2696-2702

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile and coccoid-, ovoid- or rod-shaped bacterial strain, designated KEM-5, was isolated from water sampled at an estuary environment on the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain KEM-5 fell within the clade comprising the type strains of species. Strain KEM-5 exhibited 16S rRNA gene sequence similarities of 97.01-97.66 % to the type strains of , and and of 94.18-96.95 % to the type strains of the other species. The genomicaverage nucleotide identity values of strain KEM-5 with a non-type strain (LAMA 915) of and SW-135 were 76.04 and 74.98 %, respectively. Mean DNA-DNA relatedness values between strain KEM-5 and the type strains of , and were 10-18 %. Strain KEM-5 contained Q-10 as the predominant ubiquinone and C7, summed feature 3 (Cω7 and/or Cω6), C and Cω6 as the major fatty acids. The major polar lipids of strain KEM-5 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The DNA G+C content of strain KEM-5 was 62.4 mol%. Distinguishing phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain KEM-5 is separated from recognized species. On the basis of the data presented here, strain KEM-5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KEM-5 (=KACC 19865=KCTC 62896=NBRC 113546).
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http://dx.doi.org/10.1099/ijsem.0.003510DOI Listing
September 2019

Altererythrobacter aquimixticola sp. nov., isolated from sediment sampled at the junction between the ocean and a freshwater spring.

Authors:
Sooyeon Park Ji-Min Park Jung-Hoon Yoon

Int J Syst Evol Microbiol 2019 Aug 31;69(8):2408-2414. Epub 2019 May 31.

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile and coccoid-, ovoid- or rod-shaped bacterial strain, designated SSKS-13, was isolated from sediment sampled at the junction between the ocean and a freshwater spring at Jeju island, Republic of Korea. Strain SSKS-13 grew optimally at 37 °C and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain SSKS-13 fell within the clade comprising the type strains of Altererythrobacter species, clustering with the type strains of Altererythrobacter lauratis, Altererythrobacter palmitatis and Altererythrobacter buctensis having 16S rRNA gene sequence similarities of 97.2-97.6 %. Strain SSKS-13 exhibited 16S rRNA gene sequence similarities of less than 97.0 % to the type strains of the other recognized species. Strain SSKS-13 contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids detected in strain SSKS-13 were phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The DNA G+C content of strain SSKS-13 was 64.6 mol%. The mean DNA-DNA relatedness values of strain SSKS-13 with the type strains of A. lauratis, A. palmitatis and A. buctensis were 11.7-25.3 %. Differential phenotypic properties, together with the phylogenetic and genetic data, proved that strain SSKS-13 is distinct from recognized Altererythrobacter species. On the basis of the data presented here, strain SSKS-13 is considered to represent a novel species of the genus Altererythrobacter, for which the name Altererythrobacter aquimixticola sp. nov. is proposed. The type strain is SSKS-13 (=KACC 19863=KCTC 62900=NBRC 113545).
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http://dx.doi.org/10.1099/ijsem.0.003494DOI Listing
August 2019

Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence.

Authors:
Riddhi Nagda Pratik Shah Chang Seop Lee Sooyeon Park Seong Wook Yang

Nanomaterials (Basel) 2019 Apr 28;9(5). Epub 2019 Apr 28.

Department of Systems Biology, College of Life Science and Biotechnology, Institute of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.

DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) based sensors have gained increasing attention in past years due to their diverse applications in bioimaging, biosensing, and enzymatic assays. Given the potential of DNA/AgNCs for practical applications, the systematic studies of the fluorescent stability over an extended period is necessary. However, the correlation between nucleic acid properties and the long-term stability of DNA/AgNCs is less known. With locking-to-unlocking sensors, in which the secondary structure of DNA template is standardized, we investigated the correlation between the DNA structure and the fluorescence stability of AgNCs. Post-synthesis of DNA/AgNCs, the fluorescence, and structures of templates were monitored over three weeks. By combining the fluorescence spectroscopy with the in-gel fluorescent assay, we found that AgNCs encapsulated by dimer-structured DNA/AgNCs templates were more stable than those of hairpin-structured DNA/AgNCs templates. While the orange fluorescence from the dimer templates increased over three weeks, the red fluorescence from the hairpin templates was diminished by >80% within two days at room temperature. Further tests revealed that hairpin-encapsulated red-emissive AgNCs is more sensitive to oxidation by atmospheric oxygen compared to dimer encapsulated orange AgNCs. Our observations may provide an important clue in encapsulating photophysically more stable AgNCs by tuning the DNA secondary structures. The proposed strategy here can be essential for pragmatic applications of DNA/AgNCs templates.
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http://dx.doi.org/10.3390/nano9050667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566520PMC
April 2019

Croceicoccus ponticola sp. nov., a lipolytic bacterium isolated from seawater.

Authors:
Sooyeon Park Sung-Min Won Tae-Kwang Oh Jung-Hoon Yoon

Int J Syst Evol Microbiol 2019 Jun 16;69(6):1551-1556. Epub 2019 Apr 16.

1​Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea.

A lipolytic, Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated GM-16, was isolated from seawater around Pohang in the Republic of Korea. Strain GM-16 grew optimally at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain GM-16 fell within the clade comprising the type strains of Croceicoccus species. Strain GM-16 exhibited the highest 16S rRNA gene sequence similarity (97.7 %) to the type strain of Croceicoccus pelagius and sequence similarities of 96.3-96.7 % to the type strains of the other Croceicoccus species. Strain GM-16 contained Q-10 as the predominant ubiquinone and C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as major fatty acids. The major polar lipids of strain GM-16 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and one unidentified glycolipid. The DNA G+C content of strain GM-16 was 62.6 mol%. The mean DNA-DNA relatedness value between strain GM-16 and C. pelagius DSM 101479 was 16 %. The average nucleotide identity values between strain GM-16 and the type strains of C.roceicoccus pelagius, C.roceicoccus marinus, C.roceicoccus naphthovorans and C.roceicoccus mobilis were 83.96-84.44 %. The phylogenetic and genetic data and differential phenotypic properties indicated that strain GM-16 is separated from recognized Croceicoccus species. On the basis of the data presented here, strain GM-16 is considered to represent a novel species of the genus Croceicoccus, for which the name Croceicoccus ponticola sp. nov. is proposed. The type strain is GM-16 (=KACC 19611=KCTC 62423=NBRC 113192).
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http://dx.doi.org/10.1099/ijsem.0.003301DOI Listing
June 2019

Energy and Distance-Aware Hopping Sensor Relocation for Wireless Sensor Networks.

Authors:
Moonseong Kim Sooyeon Park Woochan Lee

Sensors (Basel) 2019 Apr 1;19(7). Epub 2019 Apr 1.

Department of Electrical Engineering, Incheon National University, Incheon 22012, Korea.

Recent advances in big data technology collecting and analyzing large amounts of valuable data have attracted a lot of attention. When the information in non-reachable areas is required, IoT wireless sensor network technologies have to be applied. Sensors fundamentally have energy limitations, and it is almost impossible to replace energy-depleted sensors that have been deployed in an inaccessible region. Therefore, moving healthy sensors into the sensing hole will recover the faulty sensor area. In rough surfaces, hopping sensors would be more appropriate than wheel-driven mobile sensors. Sensor relocation algorithms to recover sensing holes have been researched variously in the past. However, the majority of studies to date have been inadequate in reality, since they are nothing but theoretical studies which assume that all the topology in the network is known and then computes the shortest path based on the nonrealistic backing up knowledge-The topology information. In this paper, we first propose a distributed hopping sensor relocation protocol. The possibility of movement of the hopping sensor is also considered to recover sensing holes and is not limited to applying the shortest path strategy. Finally, a performance analysis using OMNeT++ has demonstrated the solidification of the excellence of the proposed protocol.
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http://dx.doi.org/10.3390/s19071567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479443PMC
April 2019

Altererythrobacter insulae sp. nov., a lipolytic bacterium isolated from a tidal flat.

Authors:
Sooyeon Park Ji-Min Park Tae-Kwang Oh Jung-Hoon Yoon

Int J Syst Evol Microbiol 2019 Apr 31;69(4):1009-1015. Epub 2019 Jan 31.

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A lipolytic, Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated BPTF-M16, was isolated from tidal flat sediment on the Yellow Sea in the Republic of Korea. Strain BPTF-M16 grew optimally at 30 °C and in the presence of 2.0-3.0 % (w/v) NaCl. A phylogenetic tree of 16S rRNA gene sequences showed that strain BPTF-M16 fell within the clade comprising the type strains of Altererythrobacter species. Strain BPTF-M16 exhibited 16S rRNA gene sequence similarity values of 98.0 and 97.1 % to the type strains of Altererythrobacterishigakiensis and Altererythrobactermarinus, respectively, and of less than 97.0 % to the type strains of the other recognized species. Strain BPTF-M16 contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids detected in strain BPTF-M16 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and one unidentified glycolipid. Mean DNA-DNA relatedness values of strain BPTF-M16 with the type strains of A. ishigakiensis and A. marinus were 22 and 13 %, respectively. The average nucleotide identity value between strain BPTF-M16 and the type strain of A. ishigakiensis was 76.80 %. Differential phenotypic properties, together with the phylogenetic and genetic data, revealed that strain BPTF-M16 is separated from recognized Altererythrobacter species. On the basis of the data presented here, strain BPTF-M16 is considered to represent a novel species of the genus Altererythrobacter, for which the name Altererythrobacter insulae sp. nov. is proposed. The type strain is BPTF-M16 (=KCTC 62421=KACC 19609=NBRC 113190).
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http://dx.doi.org/10.1099/ijsem.0.003260DOI Listing
April 2019

Roseovarius ponticola sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from seawater.

Authors:
Sooyeon Park Ji-Min Park Jung-Sook Lee Jung-Hoon Yoon

Arch Microbiol 2019 Mar 27;201(2):215-222. Epub 2018 Nov 27.

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.

A lipolytic, Gram-negative, aerobic, non-motile and pleomorphic bacterial strain, designated W-SW3, was isolated from seawater around Wando, an island of South Korea, and its taxonomic position was determined using a polyphasic approach. Its optimal growth occurred at 30 °C, pH 7.0-8.0 and 2.0-4.0% (w/v) NaCl. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain W-SW3 belonged to the genus Roseovarius of the family Rhodobacteraceae, by showing the highest 16S rRNA gene sequence similarity value (97.3%) to the type strain of Roseovarius nanhaiticus. Strain W-SW3 exhibited 16S rRNA gene sequence similarities of 93.2-95.6% to the type strains of the other Roseovarius species. Ubiquinone-10 was found as the predominant ubiquinone and Cω7c and C were found as the major fatty acids. Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid and one unidentified aminolipid were major polar lipids detected. The DNA G + C content of strain W-SW3 was 63.9 mol%. Mean DNA-DNA relatedness values of strain W-SW3 with the type strains of R. nanhaiticus and R. antarcticus were 18 and 9%, respectively. The phenotypic properties, together with the phylogenetic and genetic data, made it possible strain W-SW3 to be separated from other recognized species of the genus Roseovarius. On the basis of the data presented, strain W-SW3 represents a novel species of the genus Roseovarius, for which the name Roseovarius ponticola sp. nov. is proposed. The type strain is W-SW3 (= KCTC 62424 = KACC 19615 = NBRC 113186).
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http://dx.doi.org/10.1007/s00203-018-1599-7DOI Listing
March 2019

Pseudoruegeria insulae sp. nov., isolated from a tidal flat.

Authors:
Sooyeon Park Ji-Min Park Jung-Hoon Yoon

Int J Syst Evol Microbiol 2018 Nov 21;68(11):3587-3592. Epub 2018 Sep 21.

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated BPTF-M20, was isolated from tidal flat sediment in the Yellow Sea, Republic of Korea. Strain BPTF-M20 grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BPTF-M20 fell within the clade comprising the type strains of Pseudoruegeria species. Strain BPTF-M20 exhibited 16S rRNA gene sequence similarity values of 97.4-98.3 % to the type strains of Pseudoruegeria haliotis, Pseudoruegeria lutimaris, 'Pseudoruegeria litorisediminis' and Pseudoruegeria sabulilitoris and 96.4-96.9 % to the type strains of the other Pseudoruegeria species. Strain BPTF-M20 contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids detected in strain BPTF-M20 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content of strain BPTF-M20 was 63.2 mol%. Mean DNA-DNA relatedness values of strain BPTF-M20 with the type strains of P. haliotis, P. lutimaris, P. sabulilitoris and 'P. litorisediminis' were 18-27 %. Differential phenotypic properties, together with the phylogenetic and genetic data, revealed that strain BPTF-M20 was separated from recognized Pseudoruegeria species. On the basis of the data presented here, strain BPTF-M20 is considered to represent a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeriainsulae sp. nov. is proposed. The type strain is BPTF-M20 (=KACC 19614=KCTC 62422=NBRC 113188).
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http://dx.doi.org/10.1099/ijsem.0.003035DOI Listing
November 2018

Healthy Eating for Obese Children From Socioeconomically Disadvantaged Families: Its Ecological Factors and Strategies.

Authors:
Sooyeon Park Jina Choo Sung Ok Chang

J Transcult Nurs 2019 05 3;30(3):268-279. Epub 2018 Sep 3.

1 College of Nursing, Korea University, Seoul, South Korea.

Introduction: Researchers explored the meanings of "eating" for obese children from socioeconomically disadvantaged families and identified multilevel factors and strategies for their healthy eating based on ecological perspectives.

Methodology: Grounded on a qualitative research design, focus group interviews were conducted with each group of key stakeholders: obese children, their mothers, and social workers from public welfare systems in South Korea.

Results: "Eating" and "healthy eating" were defined as "fulfilling deprivations" and "integrating balance into their lives," respectively. Eight factors and four strategies for their healthy eating were identified at intrapersonal, interpersonal, organizational, and community/political levels.

Discussion: These findings may help nurses in Western culture understand the cultural context of obese children's eating embedded in socioeconomically disadvantaged families and public welfare systems in Asian culture. Furthermore, these findings provide specific guides to culturally relevant, multilevel strategies for promoting healthy eating in the field of childhood obesity prevention.
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http://dx.doi.org/10.1177/1043659618795037DOI Listing
May 2019

Pseudopontivivens aestuariicola gen. nov., sp. nov., a Novel Bacterium of the Class Alphaproteobacteria Isolated from a Tidal Flat.

Authors:
Sooyeon Park Jeehyun Choi Ji-Min Park Jung-Hoon Yoon

Curr Microbiol 2018 Nov 20;75(11):1516-1522. Epub 2018 Aug 20.

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.

A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57 grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57 exhibited the highest 16S rRNA gene sequence similarity value (94.2%) to the type strain of Pontivivens insulae forming a cluster in the neighbour-joining phylogenetic tree. In the maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences and the phylogenetic tress based on gyrB sequences, strain OITF-57 formed evolutionary lineages independent of those of other taxa. Strain OITF-57 contained Q-10 as the predominant ubiquinone and C ω7c as the major fatty acid. The major polar lipids of strain OITF-57 were phosphatidylcholine and phosphatidylglycerol. The DNA G + C content of strain OITF-57 was 66.0 mol%. The chemotaxonomic data and other differential phenotypic properties made it possible to distinguish strain OITF-57 from the genus Pontivivens and other phylogenetically related genera. On the basis of the data presented, strain OITF-57 constitutes a new genus and species within the class Alphaproteobacteria, for which the name Pseudopontivivens aestuariicola gen. nov., sp. nov. is proposed. The type strain is OITF-57 (= KACC 19570 = CGMCC 1.13481).
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http://dx.doi.org/10.1007/s00284-018-1553-6DOI Listing
November 2018

Pseudoruegeria litorisediminis sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from a tidal flat.

Authors:
Sooyeon Park Ji-Min Park Jung-Sook Lee Tae-Kwnag Oh Jung-Hoon Yoon

Arch Microbiol 2018 Oct 7;200(8):1183-1189. Epub 2018 Jun 7.

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A lipolytic bacterial strain, designated BPTF-M5, was isolated from a tidal flat sediment of the Yellow Sea in Republic of Korea, and identified by a polyphasic taxonomic approach. It was Gram negative, aerobic, non-motile and rod shaped. Strain BPTF-M5 grew optimally at 30 °C and in the presence of 2.0-3.0% (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BPTF-M5 fell within the clade comprising the type strains of Pseudoruegeria species. The novel strain exhibited the highest 16S rRNA gene sequence similarity value (98.6%) to Pseudoruegeria marinistellae SF-16. Strain BPTF-M5 exhibited sequence similarities of 97.0-98.5% to the type strains of five other Pseudoruegeria species and of less than 96.1% to other recognized species. Strain BPTF-M5 contained Q-10 as the predominant ubiquinone and C ω7c as the major fatty acid. Phosphatidylglycerol, one unidentified aminolipid, one unidentified lipid and one unidentified glycolipid were detected as major polar lipids in the novel strain. The DNA G + C content of strain BPTF-M5 was 63.3 mol%. DNA-DNA relatedness values between strain BPTF-M5 and the type strains of the six Pseudoruegeria species were 11.0-24.3%. The phenotypic properties of strain BPTF-M5 were clearly distinguished from those of the type strains of the six Pseudoruegeria species. Based on the polyphasic data presented, strain BPTF-M5 represents a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria litorisediminis sp. nov. is proposed. The type strain is BPTF-M5 (= KCTC 62420 = KACC 19613 = NBRC 113189).
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http://dx.doi.org/10.1007/s00203-018-1539-6DOI Listing
October 2018