Publications by authors named "Songtian Che"

7 Publications

  • Page 1 of 1

Lupeol induces autophagy and apoptosis with reduced cancer stem-like properties in retinoblastoma via phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin inhibition.

J Pharm Pharmacol 2021 Apr 9. Epub 2021 Apr 9.

Department of Ocular Fundus Disease, the Second Hospital of Jilin University, Changchun, People's Republic of China.

Objectives: To evaluate the anticancer effects of lupeol in retinoblastoma cells.

Methods: WERI-Rb-1 and Y-79 cell lines were used to evaluate the anticancer effect of lupeol. After lupeol treatment, the viability, proliferation, apoptosis, cancer stem-like properties, autophagy and in vivo tumour xenograft formation were detected.

Key Findings: In this study, lupeol decreased cell viability in both WERI-Rb-1 and Y-79 cell lines. Lupeol could also inhibit proliferation and induce apoptosis of RB cells, with increased Bax level and decreased Ki67, survivin and Bcl-2 levels. Furthermore, lupeol could suppress the spheroid formation and stem-like properties of RB cells. Moreover, LC3 II/LC3 I ratio and the levels of Beclin1 and ATG7 were increased after lupeol treatment, indicating that lupeol could induce autophagy in RB cells. Next, the inhibitory effect of lupeol on the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin pathway was observed. In tumour-bearing mice, lupeol suppressed tumour growth, and this might relate to its role in cell apoptosis, autophagy and stem-like properties.

Conclusions: Lupeol suppressed proliferation and cancer stem-like properties, and promoted autophagy and apoptosis of RB cells by restraining the PI3K/AKT/mTOR pathway.
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April 2021

Ca(MgAl)(SiAl)O:Ce,Tb Phosphors: Structure Control, Density-Functional Theory Calculation, and Luminescence Property for pc-wLED Application.

Inorg Chem 2020 Apr 9;59(7):4790-4799. Epub 2020 Mar 9.

College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.

A modified structure Ca(MgAl)(SiAl)O (denoted as CMASO) from the evolution of CaMgSiO (denoted as CMSO) codoped with Ce and Tb ions was designed successfully by solid reaction method for application in phosphor-converted white-light-emitting diode (pc-wLED). The Rietveld refinement of these two structures verified the changes derived from the replacement of some of the Mg and Si ions by Al ions. The band gaps were calculated by density-functional theory (DFT) calculation method to verify the change of Al ions replacing further, and the diffuse reflectance spectra (DRS) proved the veracity of the calculation result. The phosphors CMASO:Ce showed blue emission excited by a wider excitation wavelength from 280 nm to 370 nm. The change of structure lead to the absorbable range broaden and the emission peak shifted to longer wavelength, compared with CMSO:Ce, although the amount of emitting center was the same. The reason for these phenomena was discussed in detail. The codoped phosphors CMASO:Ce,Tb exhibited different emission colors from blue to green as the concentration of Tb ions increased. Combined with commercial red phosphor CaAlSiN:Eu and ultraviolet LED (UV-LED) chips, the selected appropriate samples achieved white emission. The correlated color temperature (CCT) was 6137 K and the color rendering index (Ra) was 80.5, indicating that they could act as potential phosphors for possible applications in pc-wLED.
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April 2020

Inhibitory role of miR-203 in the angiogenesis of mice with pathological retinal neovascularization disease through downregulation of SNAI2.

Cell Signal 2020 07 19;71:109570. Epub 2020 Feb 19.

Department of Ophthalmology, The Second Hospital of Jilin University, Changchun 130041, PR China. Electronic address:

Background: Pathological retinal neovascularization is a disease characterized by abnormal angiogenesis in retina that is a major cause of blindness in humans. Previous reports have highlighted the involvement of microRNAs (miRNAs) in retinal angiogenesis. Therefore, we aimed at exploring the mechanism underlying miR-203 regulating the progression of pathological retinal neovascularization.

Methods: Initially, the mouse model of pathological retinal neovascularization disease was established and the hypoxia-induced human retinal microvascular endothelial cells (HRMECs) were generated. Then, miR-203 and SNAI2 expression in HRMECs and retinal tissues was examined. Subsequently, the effects of miR-203 and SNAI2 on viability, migration, apoptosis and angiogenesis of HRMECs were investigated, with the expression of Bax, Ki-67, MMP-2, MMP-9, VEGF and CD34 measured. Finally, the regulation of miR-203 or SNAI2 on GSK-3β/β-catenin pathway was determined through examining the levels of phosphorylated p-GSK-3β and β-catenin.

Results: Poorly expressed miR-203 and highly expressed SNAI2 were found in HRMECs and retinal tissues of pathological retinal neovascularization. Importantly, overexpressed miR-203 or silencing SNAI2 inhibited viability, migration and angiogenesis but promoted apoptosis of HRMECs, evidenced by elevated Bax expression but reduced expression of Ki-67, MMP-2, MMP-9, VEGF and CD34. Moreover, overexpression of miR-203 was found to repress the GSK-3β/β-catenin pathway by downregulating SNAI2.

Conclusion: Collectively, this study demonstrated that overexpression of miR-203 suppressed the angiogenesis in mice with pathological retinal neovascularization disease via the inactivation of GSK-3β/β-catenin pathway by inhibiting SNAI2, which provided a novel therapeutic insight for pathological retinal neovascularization disease.
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July 2020

Robust Fovea Localization Based on Symmetry Measure.

IEEE J Biomed Health Inform 2020 08 4;24(8):2315-2326. Epub 2020 Feb 4.

Automatic fovea localization is a challenging issue. In this article, we focus on the study of fovea localization and propose a robust fovea localization method. We propose concentric circular sectional symmetry measure (CCSSM) for symmetry axis detection, and region of interest (ROI) determination, which is a global feature descriptor robust against local feature changes, to solve the lesion interference issue, i.e., fovea visibility interference from lesions, using both structure features and morphological features. We propose the index of convexity and concavity (ICC) as the convexity-concavity measure of the surface and provide a quantitative evaluation tool for ophthalmologists to learn whether the occurrence of lesion within the ROI. We propose the weighted gradient accumulation map, which is insensitive to local intensity changes and can overcome the influence of noise and contamination, to perform refined localization. The advantages of the proposed method lies in two aspects. First, the accuracy and robustness can be achieved without typical sophisticated manner, i.e., blood vessel segmentation and parabola fitting. Second, the lesion interference is considered in our plan of fovea localization. Our proposed symmetry-based method is innovative in the solution of fovea detection, and it is simple, practical, and controllable. Experiment results show that the proposed method can resist the interference of unbalanced illumination and lesions, and achieve high accuracy rate in five datasets. Compared to the state-of-the-art methods, high robustness and accuracy of the proposed method guarantees its reliability.
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August 2020

One-step fabrication of boronic-acid-functionalized carbon dots for the detection of sialic acid.

Talanta 2019 May 24;197:548-552. Epub 2019 Jan 24.

College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China. Electronic address:

Typically, sialic acids (SA) with a nine-carbon backbone are found at the glycan chain termini on the cell membranes, which play crucial roles in various physiological and pathological processes. The expression level of SA in the blood serum has been reported to correlate with various disease states among cancer. In this study, a novel approach for preparing fluorescent boronic-acid-modified carbon dots (C-dots) for the detection of SA was developed. The functionalized C-dots were synthesized by a facile, one-step hydrothermal method using 3-pyridineboronic acid as the sole carbon source. The added SA selectively recognized the C-dots, leading to the fluorescence quenching of the C-dots in a linear range of 80-4000 μM with a detection limit of 54 μM. The as-developed boronic-acid nanoprobe was successfully applied for the detection of SA in human serum samples with satisfactory results. In addition, this method afforded results within 4 min. Compared to other methods, this new proposed approach was simpler and exhibited excellent sensitivity and selectivity, demonstrating immense potential as an alternative for SA detection.
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May 2019

Knockdown of long noncoding RNA 00152 (LINC00152) inhibits human retinoblastoma progression.

Onco Targets Ther 2018 6;11:3215-3223. Epub 2018 Jun 6.

Department of Ophthalmology, The First Hospital of Jilin University, Changchun, People's Republic of China.

Background: A growing body of evidence supports the involvement of long noncoding RNA 00152 (LINC00152) in the progression and metastasis of multiple cancers. However, the exact roles of LINC00152 in the progression of human retinoblastoma (RB) remain unknown. We explored the expression and biological function of human RB.

Materials And Methods: The expression level of LINC00152 in RB tissues and cells was analyzed using quantitative real-time PCR. The function of LINC00152 was determined using a series of in vitro assays. In vivo, a nude mouse model was established to analyze the function of LINC00152. Gene and protein expressions were detected using quantitative real-time PCR and Western blot assays, respectively.

Results: The expression of LINC00152 mRNA was upregulated in RB tissues and cell lines. Knockdown of LINC00152 significantly inhibited cell proliferation, colony formation, migration, and invasion and promoted cell apoptosis and caspase-3 and caspase-8 activities in vitro, as well as suppressing tumorigenesis in vivo. We identified several genes related to proliferation, apoptosis, and invasion including Ki-67, Bcl-2, and MMP-9 that were transcriptionally inactivated by LINC00152.

Conclusion: Taken together, these data implicate LINC00152 as a therapeutic target in RB.
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June 2018

as candidate gene associated with long-term and short-term survival with primary glioblastoma.

Onco Targets Ther 2017 16;10:387-395. Epub 2017 Jan 16.

Department of Neurosurgery of the First Clinical Hospital; Department of Pathology and Laboratory Medicine, School of Medicine, University of California - Irvine, Irvine, CA, USA.

Background: Glioblastoma multiforme (GBM) is the most common malignant and lethal type of primary central nervous system tumor in humans. In spite of its high lethality, a small percentage of patients have a relatively good prognosis, with median survival times of 36 months or longer. The identification of clinical subsets of GBM associated with distinct molecular genetic profiles has made it possible to design therapies tailored to treat individual patients.

Methods: We compared microarray data sets from long-term survivors (LTSs) and short-term survivors (STSs) to screen for prognostic biomarkers in GBM patients using the WebArrayDB platform. We focused on , , and , all members of a group of 10 of the most promising, differentially regulated gene candidates. Using formalin-fixed paraffin-embedded GBM samples, we corroborated the relationship between these genes and patient outcomes using methylation-specific polymerase chain reaction (PCR) for methylation status and quantitative reverse transcription PCR for expression of these genes.

Results: Expression levels of the mRNAs of these 3 genes were higher in the GBM samples than in normal brain samples and these 3 genes were significantly upregulated in STSs compared to the levels in LTS samples (<0.01). Furthermore, Kaplan-Meier analysis showed that the expression patterns of and serve as independent prognostic indicators for overall survival (<0.01 and <0.05, respectively).

Conclusion: To our knowledge, this is the first report describing as a prognostic factor for GBM patient survival, demonstrating that increased GBM survival time correlates with decreased expression. Understanding expression patterns could aid in the creation of powerful tools to predict clinical prognoses of GBM patients.
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January 2017