Publications by authors named "Song Zhu"

133 Publications

The inhibitory mechanism of chlorogenic acid and its acylated derivatives on α-amylase and α-glucosidase.

Food Chem 2021 Oct 5;372:131334. Epub 2021 Oct 5.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:

Due to the poor lipophilicity of chlorogenic acid (CA), five CA derivatives (C2-CA, C4-CA, C6-CA, C8-CA, and C12-CA) with different lipophilicities were synthesized using acylation catalyzed by lipase in present study. The inhibitory activities and mechanisms of CA and its derivatives on α-amylase and α-glucosidase were then determined. Results showed that the inhibitory activities of CA derivatives on α-amylase and α-glucosidase were enhanced as lipophilicity increased, and the inhibitory activities of C12-CA were stronger than those of CA. IC values of C12-CA were 13.30 ± 0.26 μmol/mL for α-amylase and 3.42 ± 0.10 μmol/mL for α-glucosidase. C12-CA possessed the smallest K and K values, and its inhibitory actions on α-amylase and α-glucosidase were stronger than those of CA and the other derivatives. Effects of C12-CA on microenvironments of amino acid residues and secondary structures of α-amylase and α-glucosidase were greater than those of CA and the other derivatives.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.131334DOI Listing
October 2021

Neonatal LPS Administered Before Sensitization Reduced the Number of Inflammatory Monocytes and Abrogated the Development of OVA-Induced Th2 Allergic Airway Inflammation.

Front Immunol 2021 22;12:725906. Epub 2021 Sep 22.

Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

It is becoming increasingly clear that environment factors during early life play a pivotal role in the development of allergic asthma. Among these, a traditional farm is one of the strongest protective environments, and the protective effects have been, at least in part, attributed to the high-level exposure to lipopolysaccharide (LPS) on farms. However, the underlying mechanisms remain elusive, especially in ovalbumin (OVA)-induced neonatal allergic asthma model. Here, we used the OVA-induced asthma model in two age groups, neonatal and adult, when mice were first sensitized with peritoneal OVA/alum as neonates and adults, respectively. LPS was injected in the peritoneal cavity before OVA/alum sensitization. The effects of LPS treatment on allergic airway inflammation in the lung and the immune milieu in the peritoneal cavity were determined and compared between these two age groups. We found that LPS treatment abrogated the development of Th2 allergic airway responses in the neonatal group. In the adult group, the ameliorated Th2 allergic responses were accompanied with Th17 responses and neutrophil infiltration upon LPS treatment. We further investigated the immune milieu in the peritoneal cavity to elucidate the underlying mechanisms of this age-dependent difference. Our data show that in neonatal mice, LPS treatment significantly reduced the number of inflammatory monocytes in the peritoneal cavity. In the adult group, LPS treatment shifted the function of these cells which associated with Th1 and Th17 polarization. Our results provide more evidence that immunity in early life is distinct from that in adults, especially in the peritoneal cavity, and emphasize the importance of timing for the intervention of allergic asthma. Our results suggest that LPS treatment during early life is protective for the development of Th2 allergic responses. On the other hand, it might lead to a more severe phenotype of asthma when dampening the Th2 responses in adult mice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.725906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8493091PMC
September 2021

Structural characterization and antioxidant property of enzymatic-transesterification derivatives of (-)-epigallocatechin-3-O-gallate and vinyl laurate.

J Food Sci 2021 Oct 23;86(10):4717-4729. Epub 2021 Sep 23.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

(-)-Epigallocatechin-3-O-gallate(EGCG) was enzymatically modified to enhance the lipophilicity and the antioxidant property. The determination of optimal reaction conditions are as follows: Lipase DF "Amano" 15 and acetone were used as catalyst and solvent, respectively. Equal molar of EGCG and vinyl laurate (1:1); lipase addition of 6.0% (w/w of total substrates); reaction temperature of 50°C and reaction time of 96 h, which obtained the conversion rate of EGCG at 80.1%. The structure of EGCG lauroyl derivatives were 5″-O-lauroyl-EGCG, 3″,5″-2-O-lauroyl-EGCG, and 5',3″,5″-3-O-lauroyl-EGCG, identified by high-performance liquid chromatography-mass spectrometry (HPLC-MS) and nuclear magnetic resonance (NMR). Compared with the logP of precursor EGCG (0.69 ± 0.03), the logP of EGCG lauroyl derivatives was 1.37 ± 0.19, 2.27 ± 0.33, and 3.28 ± 0.37, increasing by 0.98, 2.28, and 3.75 times, respectively (p < 0.05), suggesting the grafted fatty acid chains make EGCG derivatives more lipophilic, and the lipid solubility gradually increased as the number of substituents increased. Furthermore, EGCG lauroyl derivatives had excellent lipid oxidation than that of EGCG. The POVs (peroxide values) of soybean oil with mono-, di-, tri-lauroyl EGCG were significantly reduced by 42%, 47%, and 57% than that of EGCG at 21 days, respectively, indicating the antioxidative inhibition of these derivatives decreased with the increase in substituents. This indicates that these derivatives have broad prospects of the antioxidant application while improving their solubility properties in lipophilic environments/high-fat food. Practical Application: The lipophilic esterification reaction of EGCG catalyzed by new catalytic lipase DF "Amano" 15 was carried out in a non-aqueous solvent.Various reaction factors on a higher conversion rate of EGCG lauroyl derivatives were evaluated. The lipophilicity and antioxidant properties of EGCG lauroyl derivatives were much excellent than that of parent EGCG.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1750-3841.15894DOI Listing
October 2021

Self-Assembled Biophotonic Lasing Network Driven by Amyloid Fibrils in Microcavities.

ACS Nano 2021 09 17;15(9):15007-15016. Epub 2021 Sep 17.

School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.

Self-assembled biological structures have played a significant role in many living systems for its functionality and distinctiveness. Here, we experimentally demonstrate that the random dynamic behavior of strong light-matter interactions in complex biological structures can provide hidden information on optical coupling in a network. The concept of biophotonic lasing network is therefore introduced, where a self-assembled human amyloid fibril network was confined in a Fabry-Perot optical cavity. Distinctive lasing patterns were discovered from self-assembled amyloids with different structural dimensions (0D, 1D, 2D, and 3D) confined in a microcavity. Network laser emission exhibiting evidence of light coupling at different wavelengths and locations was spectrally resolved. Dynamic changes of lasing patterns can therefore be interpreted into a graph to reveal the optical correlation in biophotonic networks. Our findings indicate that each graph represents the highly unclonable features of a self-assembled network which can sensitively respond to environmental stimulus. This study offers the potential for studying dynamic biological networks through amplified interactions, shedding light on the development of biologically controlled photonic devices, biosensing, and information encryption.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c05266DOI Listing
September 2021

Sphingolipid profiles and their relationship with inflammatory factors in asthmatic patients of different sexes.

Chronic Dis Transl Med 2021 Sep 13;7(3):199-205. Epub 2021 May 13.

Department of Pulmonary and Critical Care Medicine, Peking University Third Hospital, Beijing 100191, China.

Background: Asthma is a heterogeneous disease with distinct prevalence and manifestation between sexes. This study was to identify sex-specific features of asthma via metabolomic analysis of sphingolipids.

Methods: Forty-two asthma patients (27 women and 15 men) admitted to the Peking University Third Hospital from January 2015 to December 2015 were enrolled. Peripheral venous blood was collected for metabolomic analysis by targeted liquid chromatography-mass spectrometry. Sex hormones(estradiol, progesterone, testosterone, and androstenedione) and multiple inflammatory factors (periostin, leptin, IgE, IL-4, IL-5, IL-10, IL-13, IL-17A, and IFN-γ) were also assessed. The eosinophil percentage in induced sputum was also detected. All these data were applied to comparative analysis between sexes.

Results: Testosterone was negatively related to periostin ( = -0.420,  = 0.009) and IL-5 ( = -0.540,  = 0.012), while estradiol was positively related to the blood eosinophil percentage ( = 0.384,  = 0.025). Among the eighteen species of sphingolipids detected in the 42 patients, five ceramide (Cer) species (Cer16:0, Cer:20:0, Cer22:0, Cer24:0, and Cer26:0) and one sphingomyelin (SM) species (SM38:0) were significantly higher in male than in female patients. Further investigation found that the correlation between Cer20:0 and IL-5 was positive in males ( = 0.943,  = 0.005) but negative in females ( = -0.561,  = 0.030).

Conclusions: Testosterone was negatively correlated with eosinophil inflammatory factors, but estradiol was positively correlated. Male asthma patients had higher ceramide and sphingomyelin levels than female patients. Different sexes had opposite correlations with ceramide and IL-5, respectively, suggesting that therapeutic strategies targeting ceramide should be different between sexes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cdtm.2021.04.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413120PMC
September 2021

Comparison of bond strength of universal adhesives using different etching modes: A systematic review and meta-analysis.

Dent Mater J 2021 Sep 2. Epub 2021 Sep 2.

Department of Prosthodontics, School and Hospital of Stomatology, Jilin University.

This review aims to evaluate whether the etch-and-rinse or self-etch mode is the better protocol for dentin adhesion by universal adhesives. A total of 15 articles were included in the meta-analysis. Two reviewers performed a literature search up to October 2020in four databases: PubMed, Web of Science, Embase, and the Cochrane Library. Without considering the difference in aging mode, the analysis of the immediate and long-term bond strength of dentin showed that there was no statistical significance between the etch-and-rinse and self-etch mode of universal adhesive, and the long-term bond strength decreased relative to the immediate. In vitro studies suggest that prior acid etching did not improve bond performance. Whether from the perspective of long-term bonding performance or simplifying operating procedures, the self-etch mode is preferred.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4012/dmj.2021-111DOI Listing
September 2021

Influence of Selenium Biofortification on the Growth and Bioactive Metabolites of .

Foods 2021 Aug 12;10(8). Epub 2021 Aug 12.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education & School of Biotechnology, Jiangnan University, Wuxi 214122, China.

Selenium biofortification of edible and medicinal mushrooms is an effective way to produce selenium-enriched food supplements. is the typical one with excellent biological activity. This study investigated growth and bioactive metabolites alterations during liquid culture with different concentrations of selenite. Low selenium levels did not affect growth and mycelia morphology, whereas high selenium levels negatively influenced growth, dramatically decreased biomass, caused nucleic acid and protein leakage, damaged cell walls and membranes, and resulted in indicators such as degraded cells, a red color, and an unpleasant odor. Through headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) analysis, ten volatile Se compounds were identified in with 200 ppm selenite, which led to an odor change, whereas only three with 50 ppm selenite. SeMet was the major selenoamino acid in the 50 ppm selenite group by high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS), but more MeSeCys was produced with 200 ppm selenite. Polysaccharide yields were promoted and inhibited with 50 and 200 ppm selenite, respectively. These results provide comprehensive insights into the effects of selenite on in liquid culture and are beneficial for functional selenium-enriched mushroom production and improving nutritive values.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/foods10081860DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391904PMC
August 2021

Identification and Antioxidant Abilities of Enzymatic-Transesterification (-)-Epigallocatechin-3--gallate Stearyl Derivatives in Non-Aqueous Systems.

Antioxidants (Basel) 2021 Aug 13;10(8). Epub 2021 Aug 13.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Vinyl stearate was added to enzymatic transesterification of (-)-Epigallocatechin-3--gallate (EGCG) to enhance its lipophilicity and antioxidant ability in a non-aqueous system. The lipase DF "Amano" 15 was used as the catalyst. The optimal reaction conditions were: acetonitrile as the solvent, the molar ratio of vinyl stearate: EGCG as 3:1, an enzyme amount of 4.0% (ratio of substrate mass), and a reaction temperature and time of 50 °C and 96 h, respectively, achieving 65.2% EGCG conversion. HPLC-MS and NMR were used to determine the structure of EGCG stearyl derivative (3″,5″-2-O-stearyl-EGCG). The lipophilicity of EGCG stearyl derivatives (3.49 ± 0.34) was higher (5.06 times) than that of the parent EGCG (0.69 ± 0.08). Furthermore, EGCG stearyl derivatives had excellent lipid oxidation compared with BHT, BHA, and parent EGCG. The POVs of soybean oil with EGCG stearyl derivatives (18.17 ± 0.92 mEq/kg) were significantly reduced (by 62.5%) at 21 d compared with those of EGCG (48.50 ± 1.23 mEq/kg). These results indicate that EGCG derivatives have broad antioxidant application prospects in lipophilic environments/high-fat food.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/antiox10081282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389292PMC
August 2021

Analysis and Design of Multivalued High-Capacity Associative Memories Based on Delayed Recurrent Neural Networks.

IEEE Trans Cybern 2021 Aug 4;PP. Epub 2021 Aug 4.

This article aims at analyzing and designing the multivalued high-capacity-associative memories based on recurrent neural networks with both asynchronous and distributed delays. In order to increase storage capacities, multivalued activation functions are introduced into associative memories. The stored patterns are retrieved by external input vectors instead of initial conditions, which can guarantee accurate associative memories by avoiding spurious equilibrium points. Some sufficient conditions are proposed to ensure the existence, uniqueness, and global exponential stability of the equilibrium point of neural networks with mixed delays. For neural networks with n neurons, m-dimensional input vectors, and 2k-valued activation functions, the autoassociative memories have (2k) storage capacities and heteroassociative memories have min storage capacities. That is, the storage capacities of designed associative memories in this article are obviously higher than the 2 and min storage capacities of the conventional ones. Three examples are given to support the theoretical results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TCYB.2021.3095499DOI Listing
August 2021

Multistability of Dynamic Memristor Delayed Cellular Neural Networks With Application to Associative Memories.

IEEE Trans Neural Netw Learn Syst 2021 Aug 4;PP. Epub 2021 Aug 4.

Recently, dynamic memristor (DM)-cellular neural networks (CNNs) have received widespread attention due to their advantage of low power consumption. The previous works showed that DM-CNNs have at most 318 equilibrium points (EPs) with n=16 cells. Since time delay is unavoidable during the process of information transmission, the goal of this article is to research the multistability of DM-CNNs with time delay, and, meanwhile, to increase the storage capacity of DM-delay (D)CNNs. Depending on the different constitutive relations of memristors, two cases of the multistability for DM-DCNNs are discussed. After determining the constitutive relations, the number of EPs of DM-DCNNs is increased to 3ⁿ with n cells by means of the appropriate state-space decomposition and the Brouwer's fixed point theorem. Furthermore, the enlarged attraction domains of EPs can be obtained, and 2ⁿ of these EPs are locally exponentially stable in two cases. Compared with standard CNNs, the dynamic behavior of DM-DCNNs shows an outstanding merit. That is, the value of voltage and current approach to zero when the system becomes stable, and the memristor provides a nonvolatile memory to store the computation results. Finally, two numerical simulations are presented to illustrate the effectiveness of the theoretical results, and the applications of associative memories are shown at the end of this article.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNNLS.2021.3099814DOI Listing
August 2021

Corrigendum: Immune Phenotyping of Patients With Acute Vogt-Koyanagi-Harada Syndrome Before and After Glucocorticoids Therapy.

Front Immunol 2021 16;12:731824. Epub 2021 Jul 16.

Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

[This corrects the article DOI: 10.3389/fimmu.2021.659150.].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.731824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323518PMC
July 2021

Modulating storage stability of binary gel by adjusting the ratios of starch and kappa-carrageenan.

Carbohydr Polym 2021 Sep 28;268:118264. Epub 2021 May 28.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address:

This study aimed to investigate the interaction mechanism of the waxy starch and kappa carrageenan (KC) gel with different ratios during co-gelatinization and storage. Water distributions, mobilities and rheological properties of the mixture gels were studied. When KC concentration was low (0.5%KC and 4% starch), the starch dominated the system, and the gel strength was the lowest. When KC concentration increased to 0.75%, the gel had the lowest change rate of fracture force, and the most homogeneous network of the freeze-dried gel was observed. When KC concentration increased to 1.0%, the gel strength was high, but the uneven structure led to the instability of the gel. Overall, the gel with 0.75%KC and 4% starch was the most stable during storage, and the exclusion of the two components to each other was the weakest, resulting in the uniform structure of the gel.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2021.118264DOI Listing
September 2021

A novel dental adhesive containing Ag/polydopamine-modified HA fillers with both antibacterial and mineralization properties.

J Dent 2021 08 4;111:103710. Epub 2021 Jun 4.

State Key Lab of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, PR China. Electronic address:

Objectives: To evaluate the antibacterial and mineralization properties of a dental adhesive containing Ag/polydopamine-modified HA (HA, hydroxyapatite) fillers.

Methods: First, an HA-polydopamine-Ag-polydopamine (HA-PDA-Ag-PDA) filler was prepared and characterized using SEM, TEM, XPS, XRD and FTIR. Then, the HA-PDA-Ag-PDA filler was mixed into an adhesive at different mass fractions (0 wt%, 0.5 wt%, 1 wt%, 2 wt%) to prepare a functional adhesive. Antibacterial and mineralization tests were carried out, and the cytotoxicity of the functional adhesive against L929 fibroblasts was also examined.

Results: The SEM, TEM, XPS, XRD and FTIR characterizations confirmed the successful preparation of the HA-PDA-Ag filler. The 1 wt% and 2 wt% functional adhesives showed the strongest bacterial inhibition effect among all the samples (p < 0.05). Obvious apatite crystals were observed in the SEM micrograph of the surface of the functional adhesive sample after immersion in artificial saliva for predetermined times (1 d, 7 d, 14 d and 28 d). There was no significant difference between the experimental group and the control group in terms of cell proliferation activity (p > 0.05).

Conclusions: The 1 wt% and 2 wt% functional adhesives demonstrated good antibacterial and mineralization properties, as well as good biocompatibility.

Clinical Significance: Functional adhesives containing Ag/polydopamine-modified HA fillers with antibacterial and mineralization capabilities might have excellent potential to enhance the stability and durability of hybrid layers and prolong the service life of dental restorations. Our study on bifunctional adhesives has paved the way for future clinical applications to increase restoration longevity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jdent.2021.103710DOI Listing
August 2021

Multistability and associative memory of neural networks with Morita-like activation functions.

Neural Netw 2021 Oct 30;142:162-170. Epub 2021 Apr 30.

Centre for Artificial Intelligence, University of Technology Sydney, Ultimo, NSW 2007, Australia. Electronic address:

This paper presents the multistability analysis and associative memory of neural networks (NNs) with Morita-like activation functions. In order to seek larger memory capacity, this paper proposes Morita-like activation functions. In a weakened condition, this paper shows that the NNs with n-neurons have (2m+1) equilibrium points (Eps) and (m+1) of them are locally exponentially stable, where the parameter m depends on the Morita-like activation functions, called Morita parameter. Also the attraction basins are estimated based on the state space partition. Moreover, this paper applies these NNs into associative memories (AMs). Compared with the previous related works, the number of Eps and AM's memory capacity are extensively increased. The simulation results are illustrated and some reliable associative memories examples are shown at the end of this paper.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neunet.2021.04.035DOI Listing
October 2021

Immune Phenotyping of Patients With Acute Vogt-Koyanagi-Harada Syndrome Before and After Glucocorticoids Therapy.

Front Immunol 2021 28;12:659150. Epub 2021 Apr 28.

Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Previous studies have established that disturbed lymphocytes are involved in the pathogenesis of Vogt-Koyanagi-Harada (VKH) syndrome. Accordingly, glucocorticoids (GCs), with their well-recognized immune-suppressive function, have been widely used for treatment of VKH patients with acute relapses. However, the systemic response of diverse immune cells to GC therapy in VKH is poorly characterized. To address this issue, we analyzed immune cell subpopulations and their phenotype, as well as cytokine profiles in peripheral blood from VKH patients (n=25) and health controls (HCs, n=21) by flow cytometry and luminex technique, respectively. For 16 patients underwent GC therapy (methylprednisolone, MP), the aforementioned measurements as well as the transcriptome data from patients before and after one-week's GC therapy were also compared to interrogate the systemic immune response to GC therapy. Lymphocyte composition in the blood was different in VKH patients and HCs. VKH patients had significantly higher numbers of T cells with more activated, polarized and differentiated phenotype, more unswitched memory B cells and monocytes, as compared to HCs. MP treatment resulted in decreased frequencies of T cells and NK cells, inhibited NK cell activation and T cell differentiation, and more profoundly, a marked shift in the distribution of monocyte subsets. Collectively, our findings suggest that advanced activation and differentiation, as well as dysregulated numbers of peripheral lymphocytes are the major immunological features of VKH, and GC therapy with MP not only inhibits T cell activation directly, but also affects monocyte subsets, which might combinatorically result in the inhibition of the pathogenic immune response.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.659150DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113950PMC
September 2021

Antioxidant activities of chlorogenic acid derivatives with different acyl donor chain lengths and their stabilities during in vitro simulated gastrointestinal digestion.

Food Chem 2021 Apr 20;357:129904. Epub 2021 Apr 20.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:

In this study, chlorogenic acid (CA) was acylated with vinyl esters of different carbon chain lengths under the action of the lipase Lipozyme RM. Five CA derivatives (C2-CA, C4-CA, C6-CA, C8-CA, and C12-CA) with different lipophilicities were obtained, and their digestive stabilities and antioxidant activities were evaluated. The lipophilicities were positively correlated with the digestive stabilities of CA derivatives. The antioxidant activities of CA derivatives did not change with the reduction of phenolic hydroxyl groups, and their capacity to scavenge 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•) and 1,1-diphenyl-2-picrylhydrazyl (DPPH•) were similar to those of CA. In cellular antioxidant activity (CAA) tests, it was found that the capacity of these derivates to cross cell membranes were enhanced upon enhancing lipophilicity, and their antioxidant activities were improved. C12-CA showed the best antioxidant activity with a median effective dose (EC) of 9.40 μg/mL, which was significantly lower than that of CA (i.e., 29.08 μg/mL).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.129904DOI Listing
April 2021

Composition and distribution of vegetation in the water level fluctuating zone of the Lantsang cascade reservoir system using UAV multispectral imagery.

PLoS One 2021 29;16(3):e0247682. Epub 2021 Mar 29.

School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan, Hubei Province, P.R.China.

With the development of a large number of tall dams and large cascade reservoir projects in the Lantsang River Basin, a large water level fluctuating zone (WLFZ) containing cascading reservoirs has formed. This newborn ecosystem is related to the sustainable development of hydropower projects, and has become a new problem to be studied urgently. Taking WLFZs in the Huangdeng, Xiaowan and Nuozhadu Reservoirs in the Lantsang River Basin as study areas, this study used multi-spectral remote-sensing field data obtained with unmanned aerial vehicles (UAVs) to ascertain the species types, coverage, distribution characteristics, dominant species and pioneer species of naturally restored vegetation. The considered data were subjected to UAV data processing, vegetation classification using multi-spectral images and a geographic information system (GIS) terrain-distribution analysis. Results show that: Polygonum Plebeium, Cynodon dactylon, Xanthium sibiricum, Ageratum conyzoides, Eleusine indica, Digitaria sanguinalis and Verbena officinalis are the dominant species of vegetation that could be naturally restored in the WLFZ; the vegetation coverage and the number of species are significantly positively correlated with the age and restoration periods of the WLFZ; the vegetation coverage of each study area increases at first, and then decreases, as a function of elevation; gentle slopes about 0-25°are more suitable for vegetation restoration. This study provides first-hand data on the natural restoration of vegetation in WLFZs, and gives a useful reference for its ecological restoration as a consequence of hydropower cascade development in the Lantsang River Basin. Finally, the study demonstrates that light UAV remote sensing is an attractive choice for investigating vegetation in reservoir WLFZs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247682PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007002PMC
September 2021

Real-time observation of the thermo-optical and heat dissipation processes in microsphere resonators.

Opt Express 2021 Jan;29(2):2402-2410

This work reports the real-time observation of the thermo-optical dynamics in silica microsphere resonators based on the dispersive time stretch technique. In general, the thermo-optical dynamics of silica microsphere resonators, including the thermal refraction and thermal expansion, can be characterized by the resonance wavelength shift, whose duration is at the millisecond timescale. However, this fast wavelength shift process cannot be directly captured by conventional spectroscopy, and only its transmission feature can be characterized by a fast-scanning laser and an intensity detector. With the advance of the time-stretch spectroscopy, whose temporal resolution is up to tens of nanoseconds, the thermo-optical dynamics can be observed in a more straight-forward way, by utilizing the pump-probe technology and mapping the resonance wavelength to the time domain. Here, the thermo-optical dynamics are explored as a function of the power and the scanning rate of the pump laser. Theoretical simulations reproduce the experimental results, revealing that the thermo-optical dynamics of silica microsphere resonators is dominated by the fast thermo-optical effect and the slow heat dissipation process to the surroundings, which leads to gradual regression of the resonance wavelength. This work provides an alternative solution for studying the thermo-optical dynamics in whispering gallery mode microresonators, which would be crucial for future applications of microresonator photonic systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.408568DOI Listing
January 2021

A community-based nurse-led medication self-management intervention in the improvement of medication adherence in older patients with multimorbidity: protocol for a randomised controlled trial.

BMC Geriatr 2021 03 2;21(1):152. Epub 2021 Mar 2.

The Nethersole School of Nursing, Faculty of Medicine, The Chinese University of Hong Kong, 6/F, Esther Lee Building, Shatin, Hong Kong SAR, China.

Background: Older patients suffering from multimorbidity are at high risk of medication nonadherence. It has been well established that self-management support is an effective strategy to enhance medication adherence for patients with chronic conditions. However, little is known about the effect of the medication self-management intervention in older patients with multimorbidity. This paper presents the protocol for a study that aims to evaluate the effectiveness of a nurse-led medication self-management intervention in improving medication adherence and health outcomes for community-dwelling older patients with multimorbidity.

Methods: The study protocol follows the recommendations of the Standard Protocol Items: Recommendations for Interventional Trials 2013 statement. This study is a multicentre, single-blind, two-arm randomised controlled trial. Older patients with multimorbidity will be recruited from three community health centres in Changsha, China. A total of 136 participants will be randomly allocated to receive usual care or usual care plus the medication self-management intervention. The intervention will be delivered by community nurses. The 6-week intervention includes three face-to-face education sessions and two weekly follow-up phone calls. Participants in the control group continue to receive all respects of usual care offered by community healthcare providers, including chronic disease management, drug prescription, referral to hospital specialists, health education and consultations regarding patients' diseases and treatments during centre visits. The primary outcome is medication adherence as measured by the 5-item Medication Adherence Report Scale. Secondary outcomes include medication self-management capacity (medication knowledge, medication beliefs, medication social support, medication skills, and medication self-efficacy), treatment experiences (medication treatment satisfaction and treatment burden), quality of life, and utilisation of healthcare services. All outcomes will be measured at baseline, immediately post-intervention, and at 3-month post-intervention.

Discussion: This study will provide evidence about the effectiveness of a medication self-management intervention, delivered by nurses, for older patients with multimorbidity and adherence problems. It is expected that the results of the study, if proven effective in improving patients' adherence and health outcomes, will provide evidence-based self-management support strategies for healthcare providers in routine chronic disease management in community settings.

Trial Registration: The trial is registered at ChiCTR.org.cn ( ChiCTR2000030011 ; date February 19, 2020).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12877-021-02097-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923480PMC
March 2021

SAM68 promotes tumorigenesis in lung adenocarcinoma by regulating metabolic conversion via PKM alternative splicing.

Theranostics 2021 19;11(7):3359-3375. Epub 2021 Jan 19.

Department of Respiratory, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan 511518, P. R. China.

A metabolic "switch" from oxidative phosphorylation to glycolysis provides tumor cells with energy and biosynthetic substrates, thereby promoting tumorigenesis and malignant progression. However, the mechanisms controlling this metabolic switch in tumors is not entirely clear. Clinical specimens were used to determine the effect of SAM68 on lung adenocarcinoma (LUAD) tumorigenesis and metastasis, and mouse models and molecular biology assays were performed to elucidate the function and underlying mechanisms and . mRNA levels were higher in LUAD tissue than in normal lung tissue, indicating that expression is upregulated in LUAD. Patients with LUAD with (n = 257) had a higher frequency of tumor recurrence ( 0.025) and recurrence-free survival ( 0.013) than did those with (n = 257). Patients with mRNA levels (n = 257) were at a higher risk for cancer-related death ( 0.006), and had shorter overall survival ( 0.044) than did those with . SAM68 promotes tumorigenesis and metastasis of LUAD cells and by regulating the cancer metabolic switch. SAM68 drives cancer metabolism by mediating alternative splicing of pyruvate kinase (PKM) pre-mRNAs, and promoting the formation of PKM2. Mechanistically, SAM68 increased the binding of the splicing repressor hnRNP A1 to exon 9 of , thereby enhancing PKM2 isoform formation and PKM2-dependent aerobic glycolysis and tumorigenesis. SAM68 promotes LUAD cell tumorigenesis and cancer metabolic programming via binding of the 351-443 aa region of SAM68 to the RGG motif of hnRNP A1, driving hnRNP A1-dependent splicing, contributing to increased oncogene PKM2 isoform formation and inhibition of PKM1 isoform formation. SAM68 is therefore a promising therapeutic target for the treatment of LUAD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.51360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847678PMC
July 2021

Observer-Based Adaptive Synchronization of Multiagent Systems With Unknown Parameters Under Attacks.

IEEE Trans Neural Netw Learn Syst 2021 Jan 29;PP. Epub 2021 Jan 29.

This article studies the observer-based adaptive synchronization of multiagent systems (MASs) with unknown parameters under attacks. First, to estimate the state of agents, the observer for MAS is introduced. When disturbance, nonlinear function, and system model uncertainty are not considered, the nominal controller is proposed to achieve synchronization and state estimation. Then, in order to eliminate the effect of unknown parameters in the disturbance, nonlinear function, and system model uncertainty, the adaptive controller with switching term is introduced. However, the attack will lead to the destruction of the network topology so as the destruction of the nominal controller. By constructing an appropriate Lyapunov function, we analyze the effect caused by attacks, and the security control law is given to make sure the synchronization of the MASs under attacks. Finally, a numerical simulation is given to verify the validness of the obtained theorem.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNNLS.2021.3051017DOI Listing
January 2021

Formation, structural characteristics and physicochemical properties of beeswax oleogels prepared with tea polyphenol loaded gelators.

Food Funct 2021 Mar;12(4):1662-1671

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. and School of Food Science and Technology, Jiangnan University, Wuxi 214122, China and International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.

In this study, a tea polyphenol (TP) loaded beeswax gelator (TP gelator) was constructed and incorporated into soybean oil to improve the oxidative stability of oleogels. The effects of TP on the structure and storage stability of oleogels were investigated. The TP gelator prepared by embedding TP into beeswax improved the dispersity of TP in lipids. The thermal characteristics and X-ray diffraction analysis showed that the components of the TP gelator coexisted homogeneously and TP was well dispersed in beeswax. The Fourier-transform infrared spectra indicated that the phenolic hydroxyl group of TP had intermolecular force with the quaternary ammonium nitrogen in the choline portion of soybean lecithin. The formation of needle-like crystals by beeswax restricted the flow of liquid oil, and TP did not participate in the construction of the internal network structure of the oleogel. In the accelerated storage experiment at 60 °C, the peroxide value of the TP loaded oleogel decreased by 60.6% at the end of the storage period compared with soybean oil. TP was successfully embedded in the oleogel without changing its structure, which not only solved the problem of poor dispersion of TP in oil, but also showed a significant inhibitory effect on lipid oxidation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0fo02772cDOI Listing
March 2021

Bioavailability and antioxidant activity of nanotechnology-based botanic antioxidants.

J Food Sci 2021 Feb 12;86(2):284-292. Epub 2021 Jan 12.

Key Laboratory of Synthetic and Biological Colloids (Ministry of Education), School of Chemical and Materials Engineering, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, China.

Botanic bioactive substances have issues with their solubility, stability, and oral bioavailability in the application, which could be improved by nanotechnologies. In another hands, green synthesis of nanoparticles (NPs) with plant extract is also a promising technology for preparation of NPs due to its safety advantage, yet the bioactive botanic substances that could be more than the assistant of the green synthesis of NPs. Based on the above concerns, this review summarized the preparation of botanic NPs with various plant extract, their solubility, stability, and oral bioavailability; specific attention has been paid to the botanic Ag/Au NPs, their capacity of antioxidant, bioavailability, antimicrobial, anti-inflammatory, and anticancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1750-3841.15582DOI Listing
February 2021

State bounding for fuzzy memristive neural networks with bounded input disturbances.

Neural Netw 2021 Feb 3;134:163-172. Epub 2020 Dec 3.

Centre for Artificial Intelligence, University of Technology Sydney, Sydney, 2007, Australia. Electronic address:

This paper investigates the state bounding problem of fuzzy memristive neural networks (FMNNs) with bounded input disturbances. By using the characters of Metzler, Hurwitz and nonnegative matrices, this paper obtains the exact delay-independent and delay-dependent boundary ranges of the solution, which have less conservatism than the results in existing literatures. The validity of the results is verified by two numerical examples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neunet.2020.11.016DOI Listing
February 2021

Circular RNA Paired-Related Homeobox 1 Promotes Gastric Carcinoma Cell Progression via Regulating MicroRNA-665/YWHAZ Axis.

Dig Dis Sci 2021 Nov 17;66(11):3842-3853. Epub 2020 Nov 17.

Department of General Surgery, Shidong Hospital, No. 999, Shiguang Road, Shanghai, 200438, China.

Background: Gastric carcinoma (GC) is a ubiquitous malignant tumor worldwide. Circular RNA paired-related homeobox 1 (circ-PRRX1), one kind of non-coding RNAs, has been reported to act as a promoter in tumor growth. This study aims to explore the effects of circ-PRRX1 on proliferation, apoptosis, and metastasis in GC and the underlying regulatory mechanisms.

Methods: The expression of circ-PRRX1, miR-665, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) mRNA was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot was used to analyze YWHAZ protein expression. 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-Htetrazolium bromide (MTT), flow cytometry, and transwell assay were carried out to assess the viability, apoptosis, migration, and invasion in GC cells. The interaction between miR-665 and circ-PRRX1 or YWHAZ was predicted by StarBase v2.0 and identified by dual-luciferase reporter system. Xenograft mouse model was employed to determine the effects of circ-PRRX1 knockdown on GC growth in vivo.

Results: Compared with normal tissues and cells, circ-PRRX1 and YWHAZ levels were upregulated, and miR-665 was downregulated in GC tissues and cells. Functionally, circ-PRRX1 knockdown inhibited the viability, migration, and invasion and promoted apoptosis in GC cells, whereas anti-miR-665 abolished these effects. Mechanistically, circ-PRRX1 was confirmed as a sponge of miR-665 to regulate YWHAZ expression. Xenograft mouse model suggested that circ-PRRX1 knockdown reduced GC cells growth in vivo.

Conclusion: Circ-PRRX1 knockdown suppressed GC development by targeting miR-665 to inhibit YWHAZ expression, and the potential molecular mechanism may provide a theoretical basis for GC therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10620-020-06705-5DOI Listing
November 2021

New Criteria on Stability of Dynamic Memristor Delayed Cellular Neural Networks.

IEEE Trans Cybern 2020 Nov 11;PP. Epub 2020 Nov 11.

Dynamic memristor (DM)-cellular neural networks (CNNs), which replace a linear resistor with flux-controlled memristor in the architecture of each cell of traditional CNNs, have attracted researchers' attention. Compared with common neural networks, the DM-CNNs have an outstanding merit: when a steady state is reached, all voltages, currents, and power consumption of DM-CNNs disappeared, in the meantime, the memristor can store the computation results by serving as nonvolatile memories. The previous study on stability of DM-CNNs rarely considered time delay, while delay is quite common and highly impacts the stability of the system. Thus, taking the time delay effect into consideration, we extend the original system to DM-D(delay)CNNs model. By using the Lyapunov method and the matrix theory, some new sufficient conditions for the global asymptotic stability and global exponential stability with a known convergence rate of DM-DCNNs are obtained. These criteria generalized some known conclusions and are easily verified. Moreover, we find DM-DCNNs have 3ⁿ equilibrium points (EPs) and 2ⁿ of them are locally asymptotically stable. These results are obtained via a given constitutive relation of memristor and the appropriate division of state space. Combine with these theoretical results, the applications of DM-DCNNs can be extended to other fields, such as associative memory, and its advantage can be used in a better way. Finally, numerical simulations are offered to illustrate the effectiveness of our theoretical results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TCYB.2020.3031309DOI Listing
November 2020

Tunable polarization beam splitter and broadband optical power sensor using hybrid microsphere resonators.

Opt Express 2020 Oct;28(22):32847-32857

Based on silica microsphere resonators embedded with iron oxide nanoparticles, we proposed and fabricated an all-optical and continuously tunable polarization beam splitter (PBS), and a broadband optical power sensor (OPS) with high sensitivity. The PBS is realized since the effective refractive indexes of the transverse-electric and transverse-magnetic polarization modes in the microsphere resonator are different. Due to the excellent photothermal effect of iron oxide nanoparticles, we realized the all-optical and continuously tunable PBS based on the hybrid microsphere resonator. A maximum polarization splitting ratio of 20 dB and a tuning range of 5 nm are achieved. Based on this mechanism, the hybrid microsphere resonator can also be used as a broadband OPS. The sensitivity of the OPS is 0.487 nm/mW, 0.477 nm/mW, and 0.398 nm/mW when the probe wavelength is 690 nm, 980 nm, and 1550 nm, respectively. With such good performances, the tunable PBS and the broadband OPS have great potential in applications such as optical routers, switches and filters.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.406083DOI Listing
October 2020

Simultaneous analysis of thirteen phytohormones in fruits and vegetables by SPE-HPLC-DAD.

Food Sci Biotechnol 2020 Nov 15;29(11):1587-1595. Epub 2020 Sep 15.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122 People's Republic of China.

Determination of phytohormones have attracted increasing attentions in food safety field. In this study, an efficient and quantitative method was developed which can simultaneously determinate thirteen phytohormones in fruits and vegetables using solid phase extraction (SPE) combined with high performance liquid chromatography-diode array detection (HPLC-DAD). The samples were extracted with 80% methanol containing 0.5% (V/V) formic acid, and the extracts were then concentrated and purified using primary secondary amine (PSA) and C18 tandem dual SPE cartridges. The analytes were separated on a Waters XBridge™ C18 column and eluated utilizing a gradient elution program of water and methanol. Mean recoveries of the thirteen analytes varied from 74.69 to 92.40%, with relative standard deviations < 3.57%. The limits of detection and quantitation were 0.005-0.018 mg/kg and 0.02-0.10 mg/kg, respectively. The phytohormones in kiwi fruit, strawberry, bean sprout, and green pepper were detected using the above method, respectively. Only the IAA content of 0.14 mg/kg was detected for the strawberry from a supermarket, which was lower than the prescribed limit in food safety standards (0.2 mg/kg).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10068-020-00814-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561641PMC
November 2020

Hydrolysis-resistant and stress-buffering bifunctional polyurethane adhesive for durable dental composite restoration.

R Soc Open Sci 2020 Jul 8;7(7):200457. Epub 2020 Jul 8.

State Key Lab of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.

A new elastic polyurethane (PU) adhesive was reported in this study to improve the stability and durability of the dental adhesion interface. A polyurethane oligomer was synthesized by the solution polymerization method, and a diluent and solvent were added to prepare PU adhesives. The water sorption, water solubility, contact angle, thermal stability, degree of conversion and mechanical properties of the PU adhesives were evaluated. Experimental applications for tooth restoration (microtensile bond strength and microleakage) were also performed, and cytotoxicity test was carried out. The water sorption and solubility of the PU adhesives were significantly lower than those of three commercial adhesives. The microtensile bond strength of the PU adhesives was improved after thermocycling test, and the extent of microleakage was diminished when compared with that of commercial adhesives. Biocompatibility testing demonstrated that the PU adhesive was non-toxic to L929 fibroblasts. This study shows the ability of PU adhesive to improve the stability and durability of the dental adhesion interface and may refocus the attention of scientists from rigid bonding to flexible bonding for dental adhesion, and it sheds light on a new strategy for the stable and durable bonding interface of dentine adhesives.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1098/rsos.200457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428285PMC
July 2020

Development and validation of a self-management scale of type 1 diabetes for Chinese adults.

J Int Med Res 2020 Aug;48(8):300060520947588

Clinic Nursing Teaching and Research Section, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China.

Objective: Self-management is beneficial for improving health outcomes in adults with type 1 diabetes. However, there are no validated instruments to assess self-management in Chinese adults with type 1 diabetes. The aim of this study was to develop and validate the Self-Management of Type 1 Diabetes for Chinese Adults (SMOD-CA) scale.

Methods: Qualitative and quantitative methods were used to develop the SMOD-CA.We conducted a literature review and semi-structured interviews to generate an initial item pool. An expert panel examined the content validity. We conducted a cross-sectional survey to evaluate scale reliability and validity. A total of 243 participants were recruited. Exploratory factor analyses were used to test the construct validity, and internal consistency and test-retest reliability were assessed.

Results: The expert panel determined that the SMOD-CA content validity index was satisfactory. The final 30-item scale consisted of four factors explaining 49.50% of the total variance in the data. Cronbach's α was 0.901 for the total scale and 0.911 for test-retest reliability.

Conclusions: The SMOD-CA demonstrated good reliability and validity. The scale is a credible and effective instrument that can be used by social workers and health care professionals to assess self-management in Chinese adults with type 1 diabetes. NCT03610984.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0300060520947588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469726PMC
August 2020
-->