Publications by authors named "Song Zhang"

1,014 Publications

  • Page 1 of 1

Blood-Repellent Performance of a Controllable Facile-Generated Superhydrophobic Surface.

ACS Appl Mater Interfaces 2021 Jun 9. Epub 2021 Jun 9.

Key Laboratory of High Efficiency and Clean Mechanical Manufacture of Ministry of Education, School of Mechanical Engineering, Shandong University, Jinan 250061, P. R. China.

Fabrication of a blood-repellent surface is essential for implantable or interventional medical devices to avoid thrombosis which can induce several serious complications. In this research, a novel micropatterned surface was fabricated a facile and cost-effective method, and then, the and blood-repellent performances of the controllable superhydrophobic surface were systematically evaluated. First, a facile and cost-effective strategy was proposed to fabricate a controllable superhydrophobic surface on a medically pure titanium substrate using an ultraviolet laser process, ultrasonic acid treatment, and chemical modification. Second, the superhydrophobicity, durability, stability, and corrosion resistance of the superhydrophobic surface were confirmed with advanced testing techniques, which display a high contact angle, low adhesion to water and blood, and excellent resistant element precipitation. Third, the platelet-rich plasma and whole blood were applied to evaluate the hemocompatibility of the superhydrophobic surface by means of an experiment, and no blood cell activation or aggregation was observed on the superhydrophobic surface. Finally, hollow tubes with an inner superhydrophobic surface were implanted into the left carotid artery of rabbits for 2 weeks to verify the biocompatibility . The superhydrophobic surface could effectively eliminate blood cell adhesion and thrombosis. No obvious inflammation or inordinate proliferation was found by histological analysis. This research provides a facile and cost-effective strategy to prepare a blood-repellent surface, which may have promising applications in implanted biomedical devices.
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http://dx.doi.org/10.1021/acsami.0c21058DOI Listing
June 2021

Ultraviolet Pump-Probe Photodissociation Spectroscopy of Electron-Rotation Coupling in Diatomics.

J Phys Chem Lett 2021 Jun 8:5534-5539. Epub 2021 Jun 8.

School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710119, China.

The electronic angular momentum projected onto the diatomic axis couples with the angular momentum of the nuclei, significantly affecting the rotational motion of the system under electronic excitations by intense lasers. In this letter, we propose a pump-probe photodissociation scheme for an accurate determination of electron-rotation coupling effects induced by the strong fields. As a showcase we study the CH molecule excited by a short intense ultraviolet pump pulse to the AΠ state, which triggers coupled rovibrational dynamics. The dynamics is observed by measuring the kinetic energy release and angular resolved photofragmentation upon photodissociation induced by the time-delayed probe pulse populating the CΣ state. Simulations of the rovibrational dynamics unravel clear fingerprints of the electron-rotation coupling effects that can be observed experimentally. The proposed pump-probe scheme opens new possibilities for the study of ultrafast dynamics following valence electronic transitions with current laser technology, and possible applications are also discussed.
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http://dx.doi.org/10.1021/acs.jpclett.1c01387DOI Listing
June 2021

Distinct Patterns of Host Adherence by Isolated from Experimental Gonorrhea.

Can J Infect Dis Med Microbiol 2021 13;2021:7865405. Epub 2021 May 13.

Department of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan, China.

( gonococci, or GC), the etiologic agent of gonorrhea, is a human-obligate bacterial pathogen. The GC surface contains pili that mediate the adherence to host cells. Studies have shown that GC pili, coded by pilin genes, undergo remarkable changes during human experimental gonorrhea, possibly generated by DNA phase variation during infection. The question that arises is whether the changes in pilins can alter the adherence capacity of to host cells. In this study, six variants initially isolated from male volunteers infected with one single clone of GC were examined for their adherence patterns with human Chang conjunctiva cells. In this study, we showed that the variants showed distinct adherence patterns to this cell line under light microscopy and scanning electron microscopy. Moreover, two reisolates showed higher adherence capacities than that of the input strain. The results provide an additional example as to how the pilus variation may play a role in the pathogenesis of .
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http://dx.doi.org/10.1155/2021/7865405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140856PMC
May 2021

Perfluorotridecanoic Acid Inhibits Leydig Cell Maturation in Male Rats in Late Puberty via Changing Testicular Lipid Component.

Chem Res Toxicol 2021 Jun 3. Epub 2021 Jun 3.

Department of Urology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang, China.

Perfluorotridecanoic acid (PFTrDA) is a long-chain (C13) perfluoroalkyl carboxylic acid. Here, we report the influence of PFTrDA exposure on the maturation of rat Leydig cells in late puberty . Male Sprague-Dawley rats were administered PFTrDA by gavage of 0, 1, 5, and 10 mg/kg/day from 35 days to 56 days postpartum. PFTrDA had no effect on body weight, testis weight, and epididymis weight. It significantly decreased the serum testosterone level after 5 and 10 mg/kg exposure, while it did not alter the serum estradiol level. The serum luteinizing hormone level was markedly reduced after 10 mg/kg PFTrDA exposure, while the follicle-stimulating hormone level was unchanged. , and transcript levels in the testis were markedly lowered in the 1-5 mg/kg PFTrDA group and the transcript level in the pituitary in the 10 mg/kg group. CYP11A1 and HSD11B1-positive Leydig cell numbers were markedly reduced after 10 mg/kg PFTrDA exposure. Testicular triglyceride and free fatty acid (palmitic acid, oleic acid, and linoleic acid) levels were significantly reduced by PFTrDA, while (up-regulation) and and ) expression were altered. AKT1 and AMPK phosphorylation was stimulated after 10 PFTrDA mg/kg exposure. In conclusion, PFTrDA delays the maturation of Leydig cells in late puberty mainly by altering the free fatty acid profile.
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http://dx.doi.org/10.1021/acs.chemrestox.0c00458DOI Listing
June 2021

Zebrafish as a Model for In-Depth Mechanistic Study for Stroke.

Transl Stroke Res 2021 May 29. Epub 2021 May 29.

Department of Anesthesiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine Shanghai Jiaotong University, 160 Pujian Rd, Shanghai, 200127, China.

Stroke is one of the world's leading causes of death and disability, posing enormous burden to the society. However, the pathogenesis and mechanisms that underlie brain injury and brain repair remain largely unknown. There's an unmet need of in-depth mechanistic research in this field. Zebrafish (Danio rerio) is a powerful tool in brain science research mainly due to its small size and transparent body, high genome synteny with human, and similar nervous system structures. It can be used to establish both hemorrhagic and ischemic stroke models easily and effectively through different ways. After the establishment of stroke model, research methods including behavioral test, in vivo imaging, and drug screening are available to explore mechanisms that underlie the brain injury and brain repair after stroke. This review focuses on the advantages and the feasibility of zebrafish stroke model, and will also introduce the key methods available for stroke studies in zebrafish, which may drive future mechanistic studies in the pursuit of discovering novel therapeutic targets for stroke patients.
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http://dx.doi.org/10.1007/s12975-021-00907-3DOI Listing
May 2021

Enhancing Dielectric Strength of Epoxy Polymers by Constructing Interface Charge Traps.

ACS Appl Mater Interfaces 2021 Jun 26;13(22):25850-25857. Epub 2021 May 26.

School of Electrical Engineering and Automation, Hefei University of Technology, Hefei 230009, P. R. China.

Epoxy polymer-based dielectric materials play a crucial role in advanced electronic devices and power equipment. However, high voltage-stress applications impose stringent requirements, such as a high dielectric strength, on epoxy polymers. Previously reported studies have shown promising material architectures in the form of epoxy polymer-nanoparticle dielectrics, which can restrict the movement of high-energy electrons by the interface charge traps associated with the various interfacial regions. However, these high-energy electrons inevitably traverse the epoxy polymer matrix and destroy the molecular structure, thereby creating a weak link for dielectric breakdown. In this study, a general strategy is developed to improve the dielectric strength by constructing interface charge traps in the molecular structure of the epoxy polymer matrix, using the -CF group in partial replacement of the -CH group. The proposed strategy increases the dielectric strength (39.5 kV mm) and surface breakdown voltage (26.9 kV) of the epoxy polymer matrix by 22.08% and 13.3%, respectively, because the interface charge trap hinders the movement of high-energy electrons. At the same time, the strategy does not degrade the mechanical and thermal properties. The results hold potential for wide application in the manufacturing of advanced future electrical and electronic equipment requiring resilience to high-voltage stress.
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http://dx.doi.org/10.1021/acsami.1c01933DOI Listing
June 2021

Hyperforin Ameliorates Imiquimod-Induced Psoriasis-Like Murine Skin Inflammation by Modulating IL-17A-Producing γδ T Cells.

Front Immunol 2021 5;12:635076. Epub 2021 May 5.

Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Hyperforin is a major active constituent of  L. extract, which is widely used for the treatment of depressive disorders. Recent studies have reported that hyperforin reduced inflammation in stroke and suppressed proliferation and differentiation in keratinocytes. Psoriasis is a chronic immune-mediated inflammatory skin disease in which the IL-23/IL-17 axis plays an important role. To investigate the underlying inflammatory mechanisms and response of hyperforin in psoriasis, we use imiquimod (IMQ)-induced mice model, cultured murine splenic γδ T cells, and HaCaT cells in this study. Data showed that hyperforin reduced epidermal thickness and decreased IMQ-induced pathological scores of cutaneous skin lesions in mice. Meanwhile we proved that hyperforin suppressed infiltration of CD3 T cells and downregulated expression of , , , , , antimicrobial peptides (AMPs) in the skin lesion. Hyperforin significantly inhibited imiquimod-induced splenomegaly, reduced serum levels of TNF-α and IL-6, and IL-17A in splenocytes and draining lymph nodes. Our study also suggested that hyperforin lessened the infiltration of γδ T cell and CCR6 γδ T cells in spleen and lymph nodes. Hyperforin also suppressed the typical psoriasis-like inflammatory responses and the infiltration of IL-17A cells in dermal γδ T cells of IMQ treated mice transferred with γδ T cells. studies, hyperforin reduced the expression and secretion of IL-17A in γδ T cells, and suppressed the activation of MAPK/STAT3 pathways in human keratinocyte HaCaT cells and γδ T cells. In conclusion, hyperforin alleviates IMQ-induced inflammation in psoriasis through suppressing the immune responses exerted by IL-17 A-producing γδ T cells and related cytokines by modulating MAPK/STAT3 pathways. Our study provided a novel therapeutic tragedy for psoriasis by which hyperforin attenuates psoriasis-related inflammatory responses.
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http://dx.doi.org/10.3389/fimmu.2021.635076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131513PMC
May 2021

A highly stable lithium metal anode enabled by Ag nanoparticle-embedded nitrogen-doped carbon macroporous fibers.

Sci Adv 2021 May 21;7(21). Epub 2021 May 21.

School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459, Singapore.

Lithium metal has been considered as an ideal anode candidate for future high energy density lithium batteries. Herein, we develop a three-dimensional (3D) hybrid host consisting of Ag nanoparticle-embedded nitrogen-doped carbon macroporous fibers (denoted as [email protected]) with selective nucleation and targeted deposition of Li. The 3D macroporous framework can inhibit the formation of dendritic Li by capturing metallic Li in the matrix as well as reducing local current density, the lithiophilic nitrogen-doped carbons act as homogeneous nucleation sites owing to the small nucleation barrier, and the Ag nanoparticles improve the Li nucleation and growth behavior with the reversible solid solution-based alloying reaction. As a result, the [email protected] composite enables a dendrite-free Li plating/stripping behavior with high Coulombic efficiency for more than 500 cycles. When this anode is coupled with a commercial LiFePO cathode, the assembled full cell manifests high rate capability and stable cycling life.
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http://dx.doi.org/10.1126/sciadv.abg3626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139591PMC
May 2021

Highly Enantioselective Synthesis of Phosphorus-containing ε-Benzosultams via Bifunctional Phosphonium Salt-promoted Hydrophosphonylation.

Chemistry 2021 May 19. Epub 2021 May 19.

Sichuan University, College of Chemistry, 29 Wangjiang Road, 610064, Chengdu, CHINA.

ε-Benzosultam derivatives are potential drug candidates with diverse biological activities. A series of chiral ε-benzosultams bearing phosphorus functionalities were synthesized via catalytic asymmetric hydrophosphonylation in the presence of a bifunctional phosphonium salt catalyst. The desired phosphonylation products were obtained in good yields with high enantioselectivities, and scale-up reactions and further derivations were successfully accomplished. Some control experiments were also conducted to explain the plausible reaction mechanism of this chemical transformation.
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http://dx.doi.org/10.1002/chem.202101038DOI Listing
May 2021

Enhancing the Solubility of Semiconducting Polymers in Eco-Friendly Solvents with Carbohydrate-Containing Side Chains.

ACS Appl Mater Interfaces 2021 Jun 18;13(21):25175-25185. Epub 2021 May 18.

Department of Chemistry and Biochemistry, University of Windsor, Windsor, Ontario N9B 3P4, Canada.

Semiconducting polymers are at the forefront of next-generation organic electronics due to their robust mechanical and optoelectronic properties. However, their extended π-conjugation often leads to materials with low solubilities in common organic solvents, thus requiring processing in high-boiling-point and toxic halogenated solvents to generate thin-film devices. To address this environmental concern, a natural product-inspired side-chain engineering approach was used to incorporate galactose-containing moieties into semiconducting polymers toward improved processability in greener solvents. Novel isoindigo-based polymers with different ratios of galactose-containing side chains were synthesized to improve the solubilities of the organic semiconductors in alcohol-based solvents. The addition of carbohydrate-containing side chains to π-conjugated polymers was found to considerably impact the intermolecular aggregation of the materials and their microstructures in the solid state as confirmed by atomic force microscopy and grazing-incidence wide-angle X-ray scattering. The charge transport characteristics of the new semiconductors were evaluated by the fabrication of organic field-effect transistors prepared from both toxic halogenated and greener alcohol-based solvents. Importantly, the incorporation of carbohydrate-containing side chains was shown to have very little detrimental impact on the electronic properties of the polymer when processed from green solvents.
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http://dx.doi.org/10.1021/acsami.1c02860DOI Listing
June 2021

Exotoxin A-Based Immunotherapy Targeting CCK2R-Expressing Colorectal Malignancies: An In Vitro and In Vivo Evaluation.

Mol Pharm 2021 Jun 17;18(6):2285-2297. Epub 2021 May 17.

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine/Double-First Class Discipline of Human-Animal Medicine, China-Japan Union Hospital, Jilin University, Changchun 130062, China.

Cholecystokinin-2 receptor (CCK2R) has been proven to be a specific biomarker for colorectal malignancies. Immunotoxins are a valuable class of immunotherapy agents consisting of a targeting element and a bacterial or plant toxin. Previous work demonstrated that targeting CCK2R is a good therapeutic strategy for the treatment of colorectal cancer (CRC). In the present study, we developed a new version of CCK2R-targeting immunotoxin GD9P using a targeted peptide, GD9, as the binding motif and a truncated exotoxin A (PE38) as the cytokiller. BALB/c nude mice were treated with different doses of GD9P, and pharmacodynamics, pharmacokinetic, and toxicological data were obtained throughout this study. Compared to the parental immunotoxin rCCK8PE38, GD9P exhibited about 1.5-fold yield, higher fluorescence intensity, and increased antitumor activity against human CRC in vitro and in vivo. The IC values of GD9P in vitro ranged from 1.61 to 4.55 nM. Pharmacokinetic studies were conducted in mice with a of 69.315 min. When tumor-bearing nude mice were treated with GD9P at doses ≥2 mg/kg for five doses, a rapid shrinkage in tumor volume and, in some cases, complete remission was observed. A preliminary safety evaluation demonstrated a good safety profile of GD9P as a exotoxin A-based immunotherapy. The therapy in combination with oxaliplatin can increase the antitumor efficacy and reduce the toxic side effects caused by chemotherapy. In conclusion, the data support the use of GD9P as a promising immunotherapy targeting CCK2R-expressing colorectal malignancies.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00095DOI Listing
June 2021

2  kW narrow-linewidth Yb-Raman fiber amplifier.

Opt Lett 2021 May;46(10):2404-2407

In this Letter, we report a high-power narrow-linewidth Yb-Raman fiber amplifier with a high second-order Raman threshold and high intensity stability. By employing two temporally stable seed lasers, over 2 kW output power at 1120 nm is achieved at a pump power of 2.6 kW with an optical-to-optical efficiency of 76.3%. The 3 dB linewidth of the 1120 nm Raman-signal laser varies slightly from 0.41 nm to 0.53 nm, and the power ratio of the second-order Raman Stokes light is only about ${-}{46.3}\;{\rm{dB}}$ at the output power of 2 kW. The results further confirm that the technique of employing temporally stable seed lasers is superior to the power scaling of narrow-linewidth Yb-Raman fiber amplifiers. To the best of our knowledge, it is the first demonstration of an over 2 kW narrow-linewidth fiber laser operating at 1120 nm.
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http://dx.doi.org/10.1364/OL.425714DOI Listing
May 2021

LncRNA AK087124/miR-224-5p/PTEN axis modulates endothelial cell injury in atherosclerosis through apoptosis and AKT signaling pathway.

Arch Biochem Biophys 2021 Jul 8;705:108916. Epub 2021 May 8.

Department of Cardiovascular Diseases, The Affiliated Hospital of Jiaxing University, Jiaxing, 314000, China; Jiaxing Institute of Atherosclerotic Disease, Jiaxing, Zhejiang, 314000, PR China. Electronic address:

Noncoding RNAs (ncRNAs) have been shown to play important roles in atherosclerosis-related endothelial cells dysfunction during atherosclerosis processes. In the study, our purpose was to discover new long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) via competitively interacting each other to regulate the pathogenesis process of atherosclerosis. We investigated the roles of lncRNA AK087124 and miR-224-5p in atherosclerotic pathogenesis and found that AK087124 was up-regulated while miR-224-5p was down-regulated in in the plasma and plaque from atherosclerotic mice compared with normal mice. Ox-LDL was used to establish the mouse aorta endothelial cell (MAEC) injury model. The function study indicated that knockdown of AK087124 inhibited ox-LDL induced endothelial apoptosis and inflammatory response. Bioinformatic prediction combining with luciferase assays indicated that AK087124 could sponge miR-224-5p and enhance the PTEN expression which is a target of miR-224-5p. RNA pull down assays also showed that biotin-miR-224-5p probe could interacted directly with AK087124 and PTEN. Pearson correlation analysis further demonstrated that AK087124 and PTEN expression are negatively correlated with miR-224-5p. Rescue study revealed that miR-224-5p silencing and PTEN overexpression both can reverse the effect of AK087124 on the ox-LDL induced endothelial injury. These data indicated that AK087124 and miR-224-5p could be potential biomarkers and target molecules to treatment and diagnosis for atherosclerosis.
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http://dx.doi.org/10.1016/j.abb.2021.108916DOI Listing
July 2021

Ligustilide Prevents Radiation Enteritis by Targeting Gch1/BH/eNOS to Improve Intestinal Ischemia.

Front Pharmacol 2021 22;12:629125. Epub 2021 Apr 22.

Department of Pharmacy, The Second Affiliated Hospital of Air Force Medical University, Xi'an, China.

There is a high incidence of radiation enteritis (RE) after abdominal radiotherapy. The occurrence of RE seriously affects the treatment and quality of life of patients; however, its pathogenesis is complex and there are no effective drugs for its prevention or treatment. Intestinal ischemia plays an important role in the occurrence of enteritis. Previous studies have shown that targeting GTP-cyclohydrolase 1 (Gch1) to improve intestinal ischemia could be a new strategy to prevent and treat RE. A high content of the naturally occurring phthalide derivative ligustilide (LIG) has been found in the plant drug Rhizoma Ligustici Chuanxiong for the treatment of cardiovascular diseases. The purpose of this study was to evaluate the protective effects of LIG on RE. Ionizing radiation (IR) rat and endothelial cell models were used to observe and record rat body weights and stool morphologies, measure intestinal blood perfusion by laser Doppler blood flow imaging, determine the diastolic functions of mesenteric arteries, detect the levels of Gch1/BH/eNOS pathway-related proteins and regulatory molecules in the mesenteric arteries and endothelial cells, and predict affinity by molecular docking technology. The results showed that LIG significantly improved the body weights, loose stools, intestinal villi lengths, intestinal perfusion and vasodilatory functions of IR rats. LIG also significantly improved Gch1 protein and BH levels in the mesenteric arteries and endothelial cells after IR, increased the NO content, reduced superoxide accumulation, and improved p-eNOS (Ser1177) levels in endothelial cells. LIG has good affinity for Gch1, which significantly improves its activity. These results indicate that LIG is the preferred compound for the prevention and treatment of RE by improving intestinal ischemia through the Gch1/BH/eNOS pathway. This study provides a theoretical basis and new research ideas for the development of new drugs for RE.
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http://dx.doi.org/10.3389/fphar.2021.629125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100595PMC
April 2021

The mediating effect of self-efficacy on the relationship between family functioning and quality of life among elders with chronic diseases.

Nurs Open 2021 May 7. Epub 2021 May 7.

School of Medicine, Yangzhou University, Yangzhou, China.

Aim: To explore whether self-efficacy has any positive or negative mediating effects between family functioning and quality of life among elders with chronic diseases.

Design: A cross-sectional study.

Methods: Questionnaires were collected from 516 community-dwelling elderly individuals with chronic diseases using a convenience sampling method. The questionnaires included the Self-efficacy for Managing Chronic Disease Six-Item Scale, the Family Adaptation Partnership Growth Affection Resolve Index and the MOS 36-Item Short Form Health Survey.

Results: Family functioning and self-efficacy impacted the quality of life of community-dwelling elderly individuals with chronic diseases. Family functioning was mediated by self-efficacy and had an indirect impact on quality of life. The mediating effect accounted for 62.50% of the total effect.
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http://dx.doi.org/10.1002/nop2.906DOI Listing
May 2021

Complete genome sequence of a novel citrus virus with characteristics of members of the family Tymoviridae.

Arch Virol 2021 Jul 5;166(7):2055-2058. Epub 2021 May 5.

National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.

A novel positive-stranded RNA virus provisionally named "citrus virus C" (CVC) was discovered in citrus trees displaying mottling symptoms. Its genome comprises 7,215 nucleotides (nt), excluding the 3' poly(A) tail, and contains two open reading frames (ORFs) that encode a replication-associated polyprotein (RP) and a putative coat protein (CP). The CVC genome contains a 16-nt 'marafibox', which is highly conserved in most viruses belonging to the genus Marafivirus of the same family. Sequence analysis suggested that the virus is most closely related to grapevine Red Globe virus (GRGV), which is yet to be officially classified in the family Tymoviridae. The sequence identities between CVC and GRGV in the whole genome (50.7%, nt) and CP (49.4% for amino acid, and 53.9% for nt) are lower than the thresholds (80% in the genome and 90% in the CP) for species demarcation in the family. Therefore, it is legitimate to propose that CVC is a member of new species in the family Tymoviridae.
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http://dx.doi.org/10.1007/s00705-021-05082-2DOI Listing
July 2021

Strehl Ratio and Myopia in Chinese Adolescents: The Tuyou County Pediatric Eye (TYPE) Study.

Int J Gen Med 2021 27;14:1541-1546. Epub 2021 Apr 27.

Department of Ophthalmology, Changzhi People's Hospital, Changzhi, 046000, People's Republic of China.

Aim: To investigate the relationships between property of the visual quality, Strehl ratio (SR) and the degree of myopia.

Methods: A total of 444 anatomically normal eyes of 222 adolescents were enrolled in the TYPE study. Based on spherical equivalent (SE), subjects were divided into four groups: emmetropia/control (SE: +0.75 to -0.75 D), low myopia (SE: -0.75 to -3.00D), moderate myopia (SE: -3.00 to 5.00D), high myopia (SE: <-5.00D). Axial length (AL) was measured. SR was attained with an OPD-III SCAN and calculated under a 3 mm pupil diameter.

Results: The overall SR (mean ± SD) was 0.40 ± 0.08. Among all included eyes, the SR in eyes with the emmetropia, low myopia, moderate myopia and high myopia was 0.80 ± 0.11, 0.31 ± 0.04, 0.21 ± 0.11, and 0.11 ± 0.02, respectively. Furthermore, the K2 in eyes with the emmetropia, low myopia, moderate myopia and high myopia was 43.83±1.50, 43.96±1.37, 43.4±5.52, and 45.16±1.43, respectively. Significant differences were detected within the four groups in terms of SR and K2 ( < 0.001). Multiple regression analysis confirmed that AL negatively affected SR independently ( < 0.001).

Conclusion: Our findings provide a useful basis for the conclusion that myopia affects visual quality SR in Chinese adolescents. Besides, when performing visual quality SR, axial length must be taken into consideration, as it will influence SR.
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http://dx.doi.org/10.2147/IJGM.S307898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088292PMC
April 2021

Bisphenol B stimulates Leydig cell proliferation but inhibits maturation in late pubertal rats.

Food Chem Toxicol 2021 Apr 30;153:112248. Epub 2021 Apr 30.

Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, 109 Xueyuan West Road, Wenzhou, Zhejiang, 325027, China. Electronic address:

Bisphenol B (BPB) has been used as a substitute for bisphenol A (BPA) in plastic materials. Whether BPB disrupts the male reproductive system remains unknown. Here, we report the effect of BPB on Leydig cell maturation in late puberty. Male Sprague-Dawley (35 days old) rats were gavaged with BPB at 0, 10, 100, and 200 mg/kg/day for 21 days. BPB significantly reduced body and epididymis weight at 200 mg/kg. BPB markedly decreased serum testosterone levels at 100 and 200 mg/kg and serum luteinizing hormone and follicle-stimulating hormone levels at 200 mg/kg. BPB significantly increased Leydig cell number at 100 and 200 mg/kg, while down-regulating the expression of Leydig cell genes (Cyp11a1 and Hsd3b1) at ≥100 mg/kg and up-regulating the expression of Sertoli cell genes (Pdgfra, Fshr, Sox9) and cell cycle regulators (Pcna, Ccnb1, Cdk2, and Cdk4) at 10-200 mg/kg. BPB markedly increased the phosphorylation of AKT1, AKT2, and ERK1/2 at 200 mg/kg. BPB increased the proliferation of rat immature Leydig cells via promoting the S/M2 phase shift at 100 and 1000 nM after 24-h culture in vitro. In conclusion, BPB disrupts Leydig cell maturation in late puberty by increasing Leydig cell number while inhibiting its maturation.
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http://dx.doi.org/10.1016/j.fct.2021.112248DOI Listing
April 2021

Microanchored borehole fiber optics allows strain profiling of the shallow subsurface.

Sci Rep 2021 Apr 28;11(1):9173. Epub 2021 Apr 28.

Suzhou NanZee Sensing Technology Ltd., Suzhou, 215123, Jiangsu, China.

Vertical deformation profiles of subterranean geological formations are conventionally measured by borehole extensometry. Distributed strain sensing (DSS) paired with fiber-optic cables installed in the ground opens up possibilities for acquiring high-resolution static and quasistatic strain profiles of deforming strata, but it is currently limited by reduced data quality due to complicated patterns of interaction between the buried cables and their surroundings, especially in upper soil layers under low confining pressures. Extending recent DSS studies, we present an improved approach using microanchored fiber-optic cables-designed to optimize ground-to-cable coupling at the near surface-for strain determination along entire lengths of vertical boreholes. We proposed a novel criterion for soil-cable coupling evaluation based on the geotechnical bearing capacity theory. We applied this enhanced methodology to monitor groundwater-related vertical motions in both laboratory and field experiments. Corroborating extensometer recordings, acquired simultaneously, validated fiber optically determined displacements, suggesting microanchored DSS as an improved means for detecting and monitoring shallow subsurface strain profiles.
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http://dx.doi.org/10.1038/s41598-021-88526-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080621PMC
April 2021

KCNQ Channels in the Mesolimbic Reward Circuit Regulate Nociception in Chronic Pain in Mice.

Neurosci Bull 2021 May 26;37(5):597-610. Epub 2021 Apr 26.

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, 221004, China.

Mesocorticolimbic dopaminergic (DA) neurons have been implicated in regulating nociception in chronic pain, yet the mechanisms are barely understood. Here, we found that chronic constructive injury (CCI) in mice increased the firing activity and decreased the KCNQ channel-mediated M-currents in ventral tegmental area (VTA) DA neurons projecting to the nucleus accumbens (NAc). Chemogenetic inhibition of the VTA-to-NAc DA neurons alleviated CCI-induced thermal nociception. Opposite changes in the firing activity and M-currents were recorded in VTA DA neurons projecting to the medial prefrontal cortex (mPFC) but did not affect nociception. In addition, intra-VTA injection of retigabine, a KCNQ opener, while reversing the changes of the VTA-to-NAc DA neurons, alleviated CCI-induced nociception, and this was abolished by injecting exogenous BDNF into the NAc. Taken together, these findings highlight a vital role of KCNQ channel-mediated modulation of mesolimbic DA activity in regulating thermal nociception in the chronic pain state.
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http://dx.doi.org/10.1007/s12264-021-00668-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099961PMC
May 2021

SMART transfer method to directly compare the mechanical response of water-supported and free-standing ultrathin polymeric films.

Nat Commun 2021 Apr 20;12(1):2347. Epub 2021 Apr 20.

Center for Optoelectronic Materials and Devices, School of Polymer Science and Engineering, University of Southern Mississippi, Hattiesburg, MS, 39406, USA.

Intrinsic mechanical properties of sub-100 nm thin films are markedly difficult to obtain, yet an ever-growing necessity for emerging fields such as soft organic electronics. To complicate matters, the interfacial contribution plays a major role in such thin films and is often unexplored despite supporting substrates being a main component in current metrologies. Here we present the shear motion assisted robust transfer technique for fabricating free-standing sub-100 nm films and measuring their inherent structural-mechanical properties. We compare these results to water-supported measurements, exploring two phenomena: 1) The influence of confinement on mechanics and 2) the role of water on the mechanical properties of hydrophobic films. Upon confinement, polystyrene films exhibit increased strain at failure, and reduced yield stress, while modulus is reduced only for the thinnest 19 nm film. Water measurements demonstrate subtle differences in mechanics which we elucidate using quartz crystal microbalance and neutron reflectometry.
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http://dx.doi.org/10.1038/s41467-021-22473-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058343PMC
April 2021

Serum lncRNAs in early pregnancy as potential biomarkers for the prediction of pregnancy-induced hypertension, including preeclampsia.

Mol Ther Nucleic Acids 2021 Jun 17;24:416-425. Epub 2021 Mar 17.

Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China.

Long noncoding RNAs (lncRNAs) are key mediators of biological regulation with diagnostic value as disease biomarkers. We explored serum lncRNA levels in early pregnancy as potential biomarkers of pregnancy-induced hypertension (PIH), including gestational hypertension (GH) and preeclampsia (PE). We performed a two-phase nested case-control study in pregnant women before 20 weeks' gestation (before clinical diagnosis). The screening phase assessed lncRNA expression profiles with a human lncRNA microarray in 5 pairs of serum samples (5 PE patients and 5 matched controls). The second phase validated levels of 8 candidate lncRNAs selected via the random walk method by quantitative real-time polymerase chain reaction (qRT-PCR). Serum levels of the 8 lncRNAs were markedly increased in women with PIH compared with matched normotensive pregnant (NP) women (p < 0.001), consistent with the microarray results. In addition, 7 candidate lncRNAs were correlated with PIH severity. Logistic regression analysis revealed that serum levels of ENST00000527727 (odds ratio [OR], 1.113; 95% confidence interval [CI], 1.024-1.209; p = 0.0113) and ENST00000415029 (OR, 1.126; 95% CI, 1.000-1.267; p = 0.0496) were associated with adverse pregnancy outcomes, such as fetal growth restriction (FGR) and placenta accreta of PIH. Nine pathways associated with the candidate lncRNAs had confirmed associations with PIH.
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http://dx.doi.org/10.1016/j.omtn.2021.03.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042241PMC
June 2021

T Cell Receptor Repertoires Acquired Routine Pap Testing May Help Refine Cervical Cancer and Precancer Risk Estimates.

Front Immunol 2021 2;12:624230. Epub 2021 Apr 2.

Department of Population and Data Sciences, UT Southwestern Medical Center, Dallas, TX, United States.

Cervical cancer is the fourth most common cancer and fourth leading cause of cancer death among women worldwide. In low Human Development Index settings, it ranks second. Screening and surveillance involve the cytology-based Papanicolaou (Pap) test and testing for high-risk human papillomavirus (hrHPV). The Pap test has low sensitivity to detect precursor lesions, while a single hrHPV test cannot distinguish a persistent infection from one that the immune system will naturally clear. Furthermore, among women who are hrHPV-positive and progress to high-grade cervical lesions, testing cannot identify the ~20% who would progress to cancer if not treated. Thus, reliable detection and treatment of cancers and precancers requires routine screening followed by frequent surveillance among those with past abnormal or positive results. The consequence is overtreatment, with its associated risks and complications, in screened populations and an increased risk of cancer in under-screened populations. Methods to improve cervical cancer risk assessment, particularly assays to predict regression of precursor lesions or clearance of hrHPV infection, would benefit both populations. Here we show that women who have lower risk results on follow-up testing relative to index testing have evidence of enhanced T cell clonal expansion in the index cervical cytology sample compared to women who persist with higher risk results from index to follow-up. We further show that a machine learning classifier based on the index sample T cells predicts this transition to lower risk with 95% accuracy (19/20) by leave-one-out cross-validation. Using T cell receptor deep sequencing and machine learning, we identified a biophysicochemical motif in the complementarity-determining region 3 of T cell receptor β chains whose presence predicts this transition. While these results must still be tested on an independent cohort in a prospective study, they suggest that this approach could improve cervical cancer screening by helping distinguish women likely to spontaneously regress from those at elevated risk of progression to cancer. The advancement of such a strategy could reduce surveillance frequency and overtreatment in screened populations and improve the delivery of screening to under-screened populations.
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http://dx.doi.org/10.3389/fimmu.2021.624230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050337PMC
April 2021

Broadband generation of perfect Poincaré beams via dielectric spin-multiplexed metasurface.

Nat Commun 2021 Apr 13;12(1):2230. Epub 2021 Apr 13.

National Laboratory of Solid-State Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, College of Engineering and Applied Sciences, Nanjing University, Nanjing, China.

The term Poincaré beam, which describes the space-variant polarization of a light beam carrying spin angular momentum (SAM) and orbital angular momentum (OAM), plays an important role in various optical applications. Since the radius of a Poincaré beam conventionally depends on the topological charge number, it is difficult to generate a stable and high-quality Poincaré beam by two optical vortices with different topological charge numbers, as the Poincaré beam formed in this way collapses upon propagation. Here, based on an all-dielectric metasurface platform, we experimentally demonstrate broadband generation of a generalized perfect Poincaré beam (PPB), whose radius is independent of the topological charge number. By utilizing a phase-only modulation approach, a single-layer spin-multiplexed metasurface is shown to achieve all the states of PPBs on the hybrid-order Poincaré Sphere for visible light. Furthermore, as a proof-of-concept demonstration, a metasurface encoding multidimensional SAM and OAM states in the parallel channels of elliptical and circular PPBs is implemented for optical information encryption. We envision that this work will provide a compact and efficient platform for generation of PPBs for visible light, and may promote their applications in optical communications, information encryption, optical data storage and quantum information sciences.
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http://dx.doi.org/10.1038/s41467-021-22462-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044217PMC
April 2021

Effect of quadratus lumborum block on postoperative cognitive function in elderly patients undergoing laparoscopic radical gastrectomy: a randomized controlled trial.

BMC Geriatr 2021 04 9;21(1):238. Epub 2021 Apr 9.

Department of Anesthesiology,Ningbo Beilun People's Hospital, No1288 Lushan East Road, Zhejiang, 315800, Ningbo, China.

Background: Quadratus lumborum block (QLB) is a novel and effective postoperative analgesia method for abdominal surgeries. However, whether QLB can affect early postoperative cognitive function by inhibiting surgical traumatic stress and the inflammatory response remains unclear. This study aimed to explore the effect of QLB on postoperative cognitive function in elderly patients undergoing laparoscopic radical gastrectomy.

Methods: Sixty-four elderly patients who underwent laparoscopic radical gastrectomy were randomly divided into the QLB group (Q group, n = 32) and control group (C group, n = 32). The Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) were used to measure cognitive function 1 day before and 7 days after surgery. Postoperative cognitive dysfunction (POCD) was defined as a decline of ≥ 1 SD in both tests. The visual analog scale (VAS) scores 6 h (T1), 24 h (T2), and 48 h (T3) after surgery were measured. The serum levels of high mobility group box protein 1 (HMGB1), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were evaluated 1 day before surgery (baseline), and 1 day (day 1) and 3 days after surgery (day 3). The intraoperative remifentanil dosage, sufentanil consumption 24 h after surgery, recovery time from anesthesia, and adverse effects were also compared.

Results: POCD was present in two patients in the QLB group and eight patients in the C group 7 days after surgery (6.7 % vs. 27.6 %, P = 0.032). The MMSE and MoCA scores were similar in both groups preoperatively, and the two scores were higher in the QLB group than in the C group 7 days after surgery (P < 0.05). The VAS scores were significantly lower in the Q group at all times after surgery (P < 0.05). Compared with the C group, the levels of HMGB1, TNF-α, and IL-6 were significantly decreased 1 and 3 days after surgery in the QLB group (P < 0.05). The remifentanil consumption intraoperatively and sufentanil 24 h postoperatively were significantly lower in the QLB group (P < 0.05). The recovery time from anesthesia was shorter in the QLB group (P < 0.05). No severe adverse effects occurred in either group.

Conclusions: QLB could improve postoperative cognitive function in elderly patients undergoing laparoscopic radical gastrectomy. This may be related to the suppression of the inflammatory response after surgery.

Trial Registration: Chictr.org.cn identifier ChiCTR1900027574 (Date of registry: 19/11/2019, prospectively registered).
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http://dx.doi.org/10.1186/s12877-021-02179-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033654PMC
April 2021

A Feedback Loop Involving MicroRNA-150 and MYB Regulates VEGF Expression in Brain Microvascular Endothelial Cells After Oxygen Glucose Deprivation.

Front Physiol 2021 17;12:619904. Epub 2021 Mar 17.

Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Vascular endothelial growth factor (VEGF) plays a pivotal role in regulating cerebral angiogenesis after stroke. Meanwhile, excessive VEGF expression induces increased microvascular permeability in brain, probably leading to neurological deterioration. Therefore, the appropriate level of VEGF expression is significant to the recovery of brain exposed to stroke. In this work, we demonstrate that microRNA-150 (miR-150) and its predicted target MYB form a negative feedback loop to control the level of post-stroke VEGF expression. Repression of MYB leads to decreased expression of miR-150 in brain microvascular endothelial cells (BMVECs) exposed to oxygen glucose deprivation (OGD), thus miR-150 was predicted to be down-regulated by MYB. Moreover, MYB was confirmed to be a direct target of miR-150 by using dual luciferase reporter assay. In our previous work, we have validated VEGF as another direct target of miR-150. Therefore, MYB participates in regulation of VEGF via miR-150 under OGD, forming a feedback loop with miR-150. We also find that high levels of miR-150 inhibitors combined with MYB silence contribute to further enhancement of VEGF expression in BMVECs in response to OGD. These observations suggest that the feedback loop comprised of miR-150 and MYB, which is a pivotal endogenous epigenetic regulation to control the expression levels of VEGF in BMVECs subjected to OGD.
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http://dx.doi.org/10.3389/fphys.2021.619904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010145PMC
March 2021

Combinatorial Synthesis of Novel 3/5(3,5)-(Di)nitro/chloropaeonol Carbonyl Hydrazone Derivatives as Nematicidal Agents.

Comb Chem High Throughput Screen 2021 Mar 24. Epub 2021 Mar 24.

Laboratory of Pesticidal Design & Synthesis, Department of Plant Protection, College of Horticultrue and Plant Protection, Henan University of Science and Technology, Luoyang 471023. China.

Background: Developing the high-efficiency and low-risk small-molecule green-nematocide is the key of effective control of the nematodes. Paeonol, is a naturally occurring phenolic compound, isolated from the root bark of Paeonia suffruticosa and the whole plant of Cynanchum paniculatum. Due to its crucial phenolic ketone skeleton, modern biological science research has indicated that paeonol has a wide range of biological activities. The structural modification of paeonol into paeonol carbonyl hydrazone derivatives is a potential approach for the development of novel nematodes, which showed more toxicity than paeonol. However, there are no reports on the nematicidal activity of paeonol carbonyl hydrazone derivatives to control Heterodera glycines.

Results: We always endeavor to discover and develop biorational natural products-based pesticidal agents, 4 significant intermediates and 21 novel 3/5(3,5)-(di)nitro/chloropaeonol carbonyl hydrazone derivatives were prepared, and their structures well characterized by 1H NMR, HRMS, MS, and mp. Due to the steric hindrance, the substituents on the C=N double bond of all hydrazine compounds adopted E configuration. Results of nematicidal activity revealed that, among all compounds, especially 5-nitropaeonol (5) and 3,5-dinitropaeonol (7) displayed the most potent nematicidal activity H. glycines in vivo with LC50 values of 0.0323 and 0.0367 mg/mL, respectively.

Conclusion: It suggested that for the 3/5(3,5)-(di)nitro/chloropaeonol carbonyl hydrazone derivatives, a nitro group introduced at C5 position of 1 was necessary for obtaining the potent compound as nematicidal agents. These preliminary results will pave the way for further modification of paeonol in the development of potential new nematicides.
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http://dx.doi.org/10.2174/1386207324666210324145627DOI Listing
March 2021

Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury.

Cell Rep 2021 Mar;34(12):108889

Department of Rehabilitation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

Spinal cord injury (SCI) often results in spasticity. There is currently no effective therapy for spasticity. Here, we describe a method to efficiently differentiate human pluripotent stem cells from spinal GABA neurons. After transplantation into the injured rat spinal cord, the DREADD (designer receptors exclusively activated by designer drug)-expressing spinal progenitors differentiate into GABA neurons, mitigating spasticity-like response of the rat hindlimbs and locomotion deficits in 3 months. Administering clozapine-N-oxide, which activates the grafted GABA neurons, further alleviates spasticity-like response, suggesting an integration of grafted GABA neurons into the local neural circuit. These results highlight the therapeutic potential of the spinal GABA neurons for SCI.
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http://dx.doi.org/10.1016/j.celrep.2021.108889DOI Listing
March 2021

Gut microbiota dysbiosis promotes age-related atrial fibrillation by lipopolysaccharide and glucose-induced activation of NLRP3-inflammasome.

Cardiovasc Res 2021 Mar 23. Epub 2021 Mar 23.

Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.

Aims: Aging is the most significant contributor to the increasing prevalence of atrial fibrillation (AF). The gut microbiota dysbiosis is involved in age-related diseases. However, whether the aged-associated dysbiosis contributes to age-related AF is still unknown. Direct demonstration that the aged gut microbiota is sufficient to transmit the enhanced AF susceptibility in a young host via microbiota-intestinal barrier-atria axis has not yet been reported. This study aimed to determine whether gut microbiota dysbiosis affects age-related AF.

Methods And Results: Herein, by using a fecal microbiota transplantation (FMT) rat model, we demonstrated that the high AF susceptibility of aged rats could be transmitted to a young host. Specially, we found the dramatically increased levels of circulating lipopolysaccharide (LPS) and glucose led to the up-regulated expression of NLR family pyrin domain containing 3 (NLRP3)-inflammasome, promoting the development of AF which depended on the enhanced atrial fibrosis in recipient host. Inhibition of inflammasome by a potent and selective inhibitor of the NLRP3 inflammasome, MCC950, resulted in a lower atrial fibrosis and AF susceptibility. Then we conducted cross-sectional clinical studies to explore the effect of aging on the altering trends with glucose levels and circulating LPS among clinical individuals in two China hospitals. We found that both of serum LPS and glucose levels were progressively increased in elderly patients as compared with those young. Furthermore, the aging phenotype of circulating LPS and glucose levels, intestinal structure and atrial NLRP3-inflammasome of rats were also confirmed in clinical AF patients. Finally, aged rats colonized with youthful microbiota restored intestinal structure and atrial NLRP3-inflammasome activity, which suppressed the development of aged-related AF.

Conclusions: Collectively, these studies described a novel causal role of aberrant gut microbiota in the pathogenesis of age-related AF, which indicates that the microbiota-intestinal barrier-atrial NLRP3 inflammasome axis may be a rational molecular target for the treatment of aged-related arrhythmia disease.

Translational Perspective: The current study demonstrates that aged-associated microbiota dysbiosis promotes AF in part through a microbiota-gut-atria axis. Increased AF susceptibility due to enhanced atrial NLRP3-inflammasome activity by LPS and high glucose was found in an aged FMT rat model, and also confirmed within elderly clinical individuals. In a long-term FMT rat study, the AF susceptibility was ameliorated by treatment with youthful microbiota. The present findings can further increase our understanding of aged-related AF and address a promising therapeutic strategy that involves modulation of gut microbiota composition.
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http://dx.doi.org/10.1093/cvr/cvab114DOI Listing
March 2021