Publications by authors named "Song Wang"

850 Publications

Efficient Dual-Band White-Light Emission with High Color Rendering from Zero-Dimensional Organic Copper Iodide.

ACS Appl Mater Interfaces 2021 May 4. Epub 2021 May 4.

School of Optical and Electronic Information, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.

Broad-band white-light emissions from organic-inorganic lead halide hybrids have attracted considerable attention in energy-saving solid-state lighting (SSL) applications. However, the toxicity of lead in these hybrids hinders their commercial prospects, and the low photoluminescence quantum yields (PLQYs) cannot meet the requirements for efficient lighting. Here, we report a highly efficient dual-band white-light emission from organic copper iodide, (CHN)CuI, which exhibits a high PLQY of 54.3% and excellent air stability. The single-crystalline (CHN)CuI possesses a unique zero-dimensional (0D) structure, in which the isolated [CuI] dimers are periodically embedded in the wide band gap organic framework of CHN. This perfect 0D structure can cause significant quantum confinement and strong electron-phonon coupling, which contributes to efficient emissions from self-trapped excitons (STEs). Photophysical studies revealed the presence of two self-trapped emitting states in [CuI] dimers, whose populations are highly sensitive to the temperature that governs the molecular environment for [CuI] dimers and the thermal activation energy of STEs. An ultraviolet (UV) excited white light-emitting diode fabricated using this single-phase white-light emitter exhibits a high color rendering index (CRI) of 78. The new material provides a promising emitter, having a high PLQY and a high CRI simultaneously, for SSL and display applications.
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http://dx.doi.org/10.1021/acsami.1c03881DOI Listing
May 2021

A systematic review and meta-analysis comparing arthroplasty and internal fixation in the treatment of elderly displaced femoral neck fractures.

OTA Int 2021 Mar 22;4(1):e087. Epub 2020 Dec 22.

Department of Orthopaedics.

Background: Currently, there are 2 mainstream treatments for displaced femoral neck fracture, including internal fixation and arthroplasty. However, there are still some controversial problems as to which treatment should be primarily chosen.

Methods: The relevant studies comparing arthroplasty with internal fixation were searched in the databases of PubMed, Embase, and Cochrane Library. Finally, 31 relevant randomized controlled trials were included in this meta-analysis. The quality of studies was evaluated and meta-analyses were performed using RevMan 5.3 software. We also assessed the heterogeneity among studies and publication bias via the I-squared index and forest plots.

Results: There was no significant difference between arthroplasty and internal fixation groups in patient mortality at both short-term and long-term points. However, patients treated with arthroplasty showed significantly lowered risks of reoperation both at short-term (5.6% vs 31.5%; relative risks (RR)  = 0.19; 95% CI, 0.13-0.28;  < .00001) and long-term follow-up (9.5% vs 45.9%; RR = 0.23; 95% CI, 0.17-0.33;  < .00001). Similarly, arthroplasty-treated patients demonstrated a significant decrease in the risk of postoperation complications at short-term (10.3% vs 34.4%; RR = 0.37, 95% CI, 0.24-0.57;  < .00001) and long-term follow-up (11.7% vs 42.5%; RR = 0.30, 95% CI, 0.16-0.57;  < .0002). Besides, patients in the arthroplasty group were associated with better alleviation of pain postoperation (18.3% vs 31.1%; RR = 0.50, 95% CI, 0.33-0.78;  = .002).In trial sequence analyses, all cumulative Z curves except that of mortality crossed the trial sequential monitoring boundaries and conventional boundaries, and required information size has been reached.

Conclusions: Arthroplasty leads to a lower rate of reoperation, a reduced risk of complications, and a better alleviation of postoperation pain both at short-term and long-term follow-up. Most importantly, and according to trial sequence analyses, more than enough evidence has been achieved that arthroplasty does show better outcomes than internal fixation in terms of reoperation rate, complications, and postoperation pain.

Level Of Evidence: Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
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http://dx.doi.org/10.1097/OI9.0000000000000087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016607PMC
March 2021

A Novel Blind Signal Detector Based on the Entropy of the Power Spectrum Subband Energy Ratio.

Entropy (Basel) 2021 Apr 11;23(4). Epub 2021 Apr 11.

School of Modern Post, Beijing University of Posts and Telecommunications, Beijing 100876, China.

In this paper, we present a novel blind signal detector based on the entropy of the power spectrum subband energy ratio (PSER), the detection performance of which is significantly better than that of the classical energy detector. This detector is a full power spectrum detection method, and does not require the noise variance or prior information about the signal to be detected. According to the analysis of the statistical characteristics of the power spectrum subband energy ratio, this paper proposes concepts such as interval probability, interval entropy, sample entropy, joint interval entropy, PSER entropy, and sample entropy variance. Based on the multinomial distribution, in this paper the formulas for calculating the PSER entropy and the variance of sample entropy in the case of pure noise are derived. Based on the mixture multinomial distribution, the formulas for calculating the PSER entropy and the variance of sample entropy in the case of the signals mixed with noise are also derived. Under the constant false alarm strategy, the detector based on the entropy of the power spectrum subband energy ratio is derived. The experimental results for the primary signal detection are consistent with the theoretical calculation results, which proves that the detection method is correct.
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http://dx.doi.org/10.3390/e23040448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070492PMC
April 2021

Preparation and Characterization of Paraffin/Mesoporous Silica Shape-Stabilized Phase Change Materials for Building Thermal Insulation.

Materials (Basel) 2021 Apr 3;14(7). Epub 2021 Apr 3.

State Key Laboratory of Green Building in Western China, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, China.

The use of phase change materials (PCMs) is an attractive method for energy storage and utilization in building envelopes. Here, shape-stabilized phase change materials (SS-PCMs) were prepared via direct adsorption using mesoporous silica (MS) with different pore diameters as the support matrix. The leakage properties, microstructure, chemical structure, thermophysical properties, activation energy, thermal stability and thermal storage-release characteristics of paraffin and SS-PCMs were investigated. The results show that the maximum mass proportion of paraffin in SS-PCMs is 70% when the average pore diameter of mesoporous silica is 15 nm, and the phase change temperature and latent heat of the corresponding SS-PCM are 23.6 °C and 135.4 kJ/kg, respectively. No chemical reaction occurs between mesoporous silica and paraffin and the SS-PCMs exhibit high thermal stability. The high activation energy of the paraffin (70%)/MS1 SS-PCM verifies that the shape and thermal properties can be maintained stably during phase change conversions. The time required for SS-PCMs to complete the thermal storage and release process is reduced by up to 34.0% compared with that for pure paraffin, showing a decline in the thermal conductivity of SS-PCMs after the addition of mesoporous silica. Hence, the prepared paraffin/MS SS-PCMs, in particular paraffin (70%)/MS1 SS-PCM, can be used for storing thermal energy and regulating indoor temperature in buildings.
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http://dx.doi.org/10.3390/ma14071775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038418PMC
April 2021

Bufalin inhibits peritoneal dissemination of gastric cancer through endothelial nitric oxide synthase-mitogen-activated protein kinases signaling pathway.

FASEB J 2021 May;35(5):e21601

The First Laboratory of Cancer Institute, the First Hospital of China Medical University, Shenyang, China.

Peritoneal dissemination threatens the survival of patients with gastric cancer (GC). Bufalin is an extract of traditional Chinese medicine, which has been proved to have anticancer effect. The target of bufalin in suppressing gastric cancer peritoneal dissemination (GCPD) and the underlying mechanism are still unclear. In this research, GC cell line MGC-803 and high-potential peritoneal dissemination cell line MKN-45P were treated with bufalin or L-NAME. Malignant biological behavior and protein level of GC cell lines were detected with MTT, wound healing, transwell, adhesion, and western blotting. Bioinformatics analysis and patient tissues were used to verify the role of endothelial nitric oxide synthase (NOS3) in GC. Mice model was used to assess the effect of bufalin and role of NOS3 in vivo. We found that bufalin inhibited the proliferation, invasion, and migration in GC cell lines. NOS3, which was an independent prognostic factor of GC patients, was predicted to be a potential target of bufalin. Further experiments proved that bufalin reduced the phosphorylation of NOS3, thereby inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway, and ultimately suppressed GCPD by inhibiting EMT process. In conclusion, NOS3 was a potential therapeutic target and prognostic biomarker of GC. Bufalin could suppress GCPD through NOS3-MAPK signaling pathway, which provided more evidence support for intraperitoneal perfusion of bufalin to treat GCPD.
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http://dx.doi.org/10.1096/fj.202002780RDOI Listing
May 2021

Proctotomy leak following laparoscopic total gastrectomy with transrectal specimen extraction for gastric cancer: a case report.

BMC Surg 2021 Apr 27;21(1):218. Epub 2021 Apr 27.

Department of General Surgery, Cheeloo College of Medicine, Qilu Hospital (Qingdao), Shandong University, 758 Hefei Road, 266035, Qingdao, Shandong, China.

Background: Despite increasing acceptance in colorectal surgery, natural orifice specimen extraction (NOSE) surgery for the treatment of gastric cancer is still in its infancy, especially via the transrectal approach, which was barely reported. So little is known about its complications. Here we report the first case of proctotomy leak after transrectal NOSE gastrectomy, and our experience in preventive interventions.

Case Presentation: A 62-year-old male patient complaining of upper abdominal pain who underwent open distal gastrectomy for gastric cancer one year ago was diagnosed with recurrent gastric cancer by gastroscopic biopsy. We performed laparoscopic total gastrectomy with transrectal specimen extraction on the patient. The operation was completed in a total laparoscopic approach and the specimen was extracted through a 3 cm longitudinal incision in the anterior wall of the upper rectum, then interrupted sutures were used for full-thickness closure of the rectal incision. The operative time was 470 min and intra-operative blood loss was 100 mL. The postoperative pathological examination showed pT1bN0M0 gastric adenocarcinoma. The patient developed proctotomy leak on the 10th postoperative day. We analyzed the causes of this rare complication and put forward a series of technical improvements. After failure of conservative treatment, a diverting ileostomy was created and the patient eventually recovered. We successfully prevented proctotomy leak in the subsequent 20 transrectal NOSE gastrectomies using improved techniques.

Conclusions: Proctotomy leak after transrectal specimen extraction should be considered among the complications of NOSE surgery and can be prevented by technical precautions.
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http://dx.doi.org/10.1186/s12893-021-01217-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077703PMC
April 2021

Biomimetic Ca nanogenerator based on ions interference strategy for tumour-specific therapy.

J Drug Target 2021 May 3:1-8. Epub 2021 May 3.

Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China.

Intracellular Ca ions as second messenger played key role in cell behaviour, which was often overlooked in traditional antitumor treatment. Disrupting Ca ion homeostasis by Ca overload might switch ions signal from 'regulating' to 'destroying'. Inspired by this, a biomimetic Ca nanogenerator was constructed. Briefly, the curcumin (CUR) was loaded into mesoporous calcium carbonate nanoparticles (MCC NPs), and then coated with platelet (PLT) membrane. Upon reaching tumour cells by PLT membrane-mediated tumour targeting effect, PLT@MCC/CUR would instantaneously decompose in acidic lysosomes, concurrently accompanying with Ca generation and CUR release. The CUR could further facilitate Ca release from endoplasmic reticulum (ER) and inhibit Ca efflux, aggravating Ca overload to disrupt mitochondrial Ca homeostasis for mitochondria apoptosis signalling pathway activation. Interestingly, such effect was ineffective in normal cells, realising the tumour-specific therapeutic therapy. Based on ions interference strategy, PLT@MCC/CUR herein offered synergistic combination of Ca overload therapy and chemotherapy, which would pave the way towards more effective nanotherapeutics.
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http://dx.doi.org/10.1080/1061186X.2021.1919123DOI Listing
May 2021

The hidden mediating role of innovation efficiency in coordinating development of economy and ecological environment: evidence from 283 Chinese cities.

Environ Sci Pollut Res Int 2021 Apr 24. Epub 2021 Apr 24.

Institute of the Central China Development, Wuhan University, Wuhan, 430072, China.

The advancement of technological innovations may facilitate the coordinated development of the economy and ecological environment (EGE); however, few studies have noted the role of innovation efficiency (INEF) as a bridge between economic development and EGE. To fill in this gap, this paper adopts DMSP/OLS nighttime light data to measure the level of economic development and the improved data envelopment analysis model to measure INEF, establishing an index system to quantify EGE. Then we propose the panel threshold-mediating (PTM) model that combines the panel threshold model and the mediating effect model. On these bases, considering INEF as a mediator, we use this novel PTM model to empirically test the nonlinear relationship between economic development and EGE in 283 Chinese cities from 2003 to 2018. The results indicate that (1) while economic development can improve EGE, its positive impact on EGE differs with different INEF thresholds. A higher INEF threshold indicates a stronger positive effect of economic development on EGE. (2) For the entire sample, the mediating effect of INEF between economic development and EGE is not significant. However, after applying the PTM model, we found that the mediating effect of INEF was concealed by the sample population. (3) When INEF is lower than 0.2381, its complete mediating effect is verified. When INEF is higher than 0.2381, its mediating effect is not significant. On the contrary, economic development directly improves EGE. (4) Industrial agglomeration, the scale of government expenditure, residents' education level, degree of opening up, and transport infrastructure are all conducive to EGE. These findings can help cities at different INEF levels to achieve coordinated development of economy and EGE.
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http://dx.doi.org/10.1007/s11356-021-13808-7DOI Listing
April 2021

Nomogram including chemotherapy response for prediction of intrahepatic progression-free survival in patients with colorectal liver metastasis through chemotherapy followed by radiofrequency ablation.

Int J Hyperthermia 2021 ;38(1):633-639

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Ultrasound, Peking University Cancer Hospital & Institute, Beijing, China.

Background: Radiofrequency ablation (RFA) is being considered as the favorable treatment option for unresectable colorectal cancer liver metastases (CRLM) receiving chemotherapy, yet there still exist challenges for recurrence after RFA. The present study aims to establish an effective nomogram to predict intrahepatic progression-free survival (PFS) and select RFA candidates.

Methods: Patients with unresectable CRLM treated with chemotherapy followed by RFA between 2010 and 2016 were enrolled in this study. The nomogram to predict intrahepatic PFS was established based on multivariable Cox regression analysis. The predictive performance of the nomogram was assessed according to the C-index, calibration plots and Kaplan-Meier curve.

Results: Of a total of 158 patients, the earlier new intrahepatic metastases over local tumor progression were observed in 157 patients during the follow-up, and the mean intrahepatic PFS was 16.9 ± 1.4 months in the present cohort. The optimal cutoff value of tumor size after chemotherapy was identified as 16 mm by X-tile analysis. Based on multivariate analysis, independent prognostic factors for intrahepatic PFS included primary positive lymph nodes, multiple metastases, tumor size >16 mm, no primary lesion resection, mutant KRAS and PD response after chemotherapy. The nomogram was established to predict intrahepatic PFS based on all independent factors, which achieved favorable discrimination and calibration.

Conclusion: This study firstly established the nomogram to predict intrahepatic PFS for unresectable CRLM patients receiving chemotherapy followed by RFA. It can facilitate the selection of RFA candidates, and help both surgeons and patients choose individualized regimens in the treatment decision.
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http://dx.doi.org/10.1080/02656736.2021.1912415DOI Listing
January 2021

Has ridesourcing reduced haze? An analysis using the Didi app.

Environ Sci Pollut Res Int 2021 Apr 19. Epub 2021 Apr 19.

Institute of Central China Development, Wuhan University, 430072, Wuhan, Hubei, People's Republic of China.

Environmental degradation, often evidenced by haze, has not only increased the cost of China's economic development, but its unfair distribution among regional development, social hierarchy, and generations has also reduced quality of life. Hence, this study uses cross-sectional data from Didi, a Chinese ridesourcing service company, from 2017 to study the impact mechanism of shared mobility on haze. First, it applies the generalized spatial two-stage least squares model after controlling for urban economic, geographic, transportation, meteorological, and policy variables. We then use urbanization depth (urban agglomeration) and breadth (urban sprawl) variables, which represent the intensity and scope of human activities, respectively, as mediators to test the transmission mechanism from shared mobility to haze. Finally, differences based on urban heterogeneity were tested in the end. The study finds that the scale of ridesourcing and haze show a significant U-shaped relationship: the initial development of ridesourcing has a significant effect on reducing haze, but will then intensify haze as it develops to scales such as those of Beijing and Shenzhen. Moreover, the depth of urbanization is the main transmission mechanism of haze. This conclusion is verified in Tier 3 cities and above; however, the transmission mechanism in Tier 4 and Tier 5 cities is not obvious, indicating that the scale of ridesourcing should be compatible with the scale of urban development. Therefore, the design of emission reduction policies should be considered from the perspective of urban agglomeration.
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http://dx.doi.org/10.1007/s11356-021-13934-2DOI Listing
April 2021

Laparoscopic Pancreaticoduodenectomy with Transanal Specimen Extraction for Periampullary Tumors.

Ann Surg 2021 Apr 7. Epub 2021 Apr 7.

Department of General Surgery, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, 758 Hefei Road, Qingdao, Shandong, 266035, China Department of General Surgery, Zibo Municipal Hospital, Shandong, 255400, China Department of Respiratory Medicine, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, 758 Hefei Road, Qingdao, Shandong, 266035, China.

Objective: To explore the possibility of laparoscopic pancreaticoduodenectomy (LPD) with transanal specimen extraction for periampullary tumors.

Background: Natural orifice specimen extraction (NOSE) has been widely performed in colorectal surgery. But so far there is no report regarding NOSE in LPD.

Methods: Data of 3 patients who underwent LPD with transanal specimen extraction by the same surgeon between July 2018 to March 2019 due to periampullary tumors were evaluated retrospectively.

Results: All patients underwent LPD with no conversion to open surgery. Specimens were all extracted transanally. No complications occurred except for delayed gastric emptying in one patient. The patients' anorectal function was intact and no opioids were required postoperatively. After a follow-up of 17-25 months, all patients were alive with no tumor recurrence.

Conclusion: Treatment of periampullary tumors by LPD with transanal specimen extraction is feasible and safe.
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http://dx.doi.org/10.1097/SLA.0000000000004886DOI Listing
April 2021

Subjective Family Socioeconomic Status and Peer Relationships: Mediating Roles of Self-Esteem and Perceived Stress.

Front Psychiatry 2021 24;12:634976. Epub 2021 Mar 24.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu, China.

This study explored the relationships between subjective family socioeconomic status (FSES), self-esteem, perceived stress, and perceived peer relationships among Chinese adolescents. A total of 1,353 adolescents (age range: 15-19 years) were asked to complete a questionnaire. Mediation analysis revealed that subjective FSES influenced perceived peer relationships in three ways: first, through the mediating effect of perceived stress; second, through the mediating effect of self-esteem; and third, through the serial mediating effects of perceived stress and self-esteem. The results remained significant after controlling for parental education. In addition, a contrast analysis showed no significant differences in the mediating effects of self-esteem and perceived stress. Thus, we suggest that steps should be taken to improve adolescents' self-esteem and reduce their stress through training interventions and preventive measures, to help them improve their perceived peer relationships and reduce adverse effects associated with low subjective FSES.
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http://dx.doi.org/10.3389/fpsyt.2021.634976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024469PMC
March 2021

Retrieving the deleterious mutations before extinction: genome-wide comparison of shared derived mutations in liver cancer and normal population.

Postgrad Med J 2021 Apr 9. Epub 2021 Apr 9.

Department of Interventional Radiology, The Affiliated Hospital of Qingdao University, Qingdao, China

Study Purpose: Deleterious mutations would be rapidly purged from natural populations along with the extinction of their carriers. The currently observed mutations in existing species are mostly neutral. The inaccessibility of deleterious mutations impedes the functional studies on how these mutations affect the fitness at individual level.

Study Design: The connection between the deleterious genotype and the non-adaptive phenotype could be bridged by sequencing the genome before extinction. Although this approach is no longer feasible for evolutionary biologists, it is feasible for cancer biologists by profiling the mutations in tumour samples which are so deleterious that the carriers hardly live.

Results: By comparing the derived mutation profile between normal populations and patients with liver cancer, we found that the shared mutations, which are highly deleterious, are suppressed to low allele frequencies in normal populations and tissues, but show remarkably high frequency in tumours. The density of shared mutations is negatively correlated with gene conservation and expression levels.

Conclusions: Deleterious mutations are suppressed in functionally important genes as well as in normal populations. This work deepened our understanding on how natural selection act on deleterious mutations by analogising the cancer evolution to species evolution, which are essentially the same molecular process but at different time scales.
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http://dx.doi.org/10.1136/postgradmedj-2021-139993DOI Listing
April 2021

Synonymous mutations that regulate translation speed might play a non-negligible role in liver cancer development.

BMC Cancer 2021 Apr 9;21(1):388. Epub 2021 Apr 9.

Department of interventional radiology, The Affiliated Hospital of Qingdao University, Qingdao, China.

Background: Synonymous mutations do not change the protein sequences. Automatically, they have been regarded as neutral events and are ignored in the mutation-based cancer studies. However, synonymous mutations will change the codon optimality, resulting in altered translational velocity.

Methods: We fully utilized the transcriptome and translatome of liver cancer and normal tissue from ten patients. We profiled the mutation spectrum and examined the effect of synonymous mutations on translational velocity.

Results: Synonymous mutations that increase the codon optimality significantly enhanced the translational velocity, and were enriched in oncogenes. Meanwhile, synonymous mutations decreasing codon optimality slowed down translation, and were enriched in tumor suppressor genes. These synonymous mutations significantly contributed to the translational changes in tumor samples compared to normal samples.

Conclusions: Synonymous mutations might play a role in liver cancer development by altering codon optimality and translational velocity. Synonymous mutations should no longer be ignored in the genome-wide studies.
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http://dx.doi.org/10.1186/s12885-021-08131-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033552PMC
April 2021

Psychological Status and Correlated Factors of Primary Medical Staff During the COVID-19 Outbreak in Hefei City, China.

J Multidiscip Healthc 2021 31;14:751-756. Epub 2021 Mar 31.

Lianhua Community Health Service Center, The Second Affiliated Hospital, Anhui Medical University, Hefei, Anhui, People's Republic of China.

Objective: To investigate the mental illness and correlated factors of primary medical staff during the COVID-19 outbreak in Hefei city, China.

Methods: A total of 180 primary medical staff were randomly selected from seven community hospitals in Hefei Economic and Technological Development Zone as a study group. One hundred and eighty-two health people were recruited as the control group. The self-rating Anxiety Scale (SAS), self-rating Depression Scale (SDS) and Psychological questionnaire of public health emergencies were distributed to them for evaluation.

Results: The score of SAS, SDS in study group was higher than that in control group [(35.57±10.39) vs (31.31±7.98); (44.16±8.46) vs (41.47±9.47)] (=4.371, < 0.001; =2.849, =0.005). The fear subscale and total score in the psychological questionnaire of sudden public health events were negatively correlated with age (=-0.216, =0.004; =-0.154, =0.039). Marriage was negatively correlated with depression subscales in psychological questionnaires of SAS, SDS and sudden public health events (=-0.184, =0.013; =-0.298, <0.001; =-0.161, =0.031; =-0.147, =0.049). Education level was positively correlated with the total score of a psychological questionnaire for sudden public health events (=0.151, =0.043); Logistic regression analysis showed that marital status was a protective factor of psychological abnormality.

Conclusion: It is necessary to pay attention to the psychological status of primary medical staff, especially the young unmarried medical staff.
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http://dx.doi.org/10.2147/JMDH.S289336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020133PMC
March 2021

Design of Graphene/Ionic Liquid Composites for Carbon Capture.

ACS Appl Mater Interfaces 2021 Apr 8;13(15):17511-17516. Epub 2021 Apr 8.

Department of Chemistry, University of California, Riverside, California 92521, United States.

Pore size is a crucial factor impacting gas separation in porous separation materials, but how to control the pore size to optimize the separation performance remains a challenge. Here, we propose a design of graphene/ionic liquid composites with tunable slit pore sizes, where cations and anions of ionic liquids are intercalated between graphene layers. By varying the sizes of the ions, we show from first-principles density functional theory calculations that the accessible pore size can be tuned from 3.4 to 6.0 Å. Grand canonical Monte Carlo simulations of gas sorption find that the composite materials possess high CO uptake at room temperature and 1 bar (up to ∼8.5 mmol/g). Further simulations of the sorption of gas mixtures reveal that high CO/N and CO/CH adsorption selectivities can be obtained when the accessible pore size is <5 Å. This work suggests a new strategy to achieve tunable pore sizes via the graphene/IL composites for highly selective CO/N and CO/CH adsorption.
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http://dx.doi.org/10.1021/acsami.1c01242DOI Listing
April 2021

Screening a prognosis-related target gene in patients with HER-2 positive breast cancer by bioinformatics analysis.

Authors:
Song Wang Yi Quan

Med Princ Pract 2021 Apr 6. Epub 2021 Apr 6.

Objective: HER-2 positive breast cancer has a high risk of for relapse, metastasis and drug resistance, and is related to a poor prognosis. Thus, the study objective was to determine a target gene and explore the associated molecular mechanisms in HER-2 positive breast cancer.

Methods: Three RNA expression profiles were obtained from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA), and were used to identify differentially expressed genes (DEGs) using R software. A Protein-Protein Interaction (PPI) network was constructed and hub genes were determined. Subsequently, the relationship between clinical parameters and hub genes was examined to screen target gene. Next, DNA methylation and genomic alterations of the target gene were evaluated. To further explore potential molecular mechanisms, genes co-expressed with the target gene were performed functional enrichment analysis Results: The differential expression analysis revealed 217 DEGs in HER-2 positive breast cancer tissues compared to normal breast tissues. RRM2 was the only hub gene closely associated with lymphatic metastasis and prognosis in HER-2 positive breast cancer. Additionally, RRM2 was frequently often amplified and negatively associated with the methylation level. Functional enrichment analysis showed that the co-expression genes were mainly involved in cell cycle.

Conclusions: The present study identified RRM2 as a target gene associated with the initiation, progression and prognosis of HER-2 positive breast cancer, which may contribute to provide a new biomarker and therapeutic target.
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http://dx.doi.org/10.1159/000516322DOI Listing
April 2021

Effects of psychosocial factors on posttraumatic growth among lung cancer patients: A structural equation model analysis.

Eur J Cancer Care (Engl) 2021 Apr 4:e13450. Epub 2021 Apr 4.

West China Hospital/West China School of Nursing, Sichuan University, Chengdu, China.

Objective: The purpose of this study was to investigate how locus of control, coping strategies, emotion regulation strategies and social support affect posttraumatic growth.

Methods: A cross-sectional study design was adopted. A convenience sample of 540 patients with lung cancer was recruited from November 2016 to July 2017 at two tertiary grade A hospitals in China. The participants completed a series of questionnaires. Structural equation modelling was used to explore the effects of psychosocial factors on posttraumatic growth.

Results: Social support had a direct positive effect on posttraumatic growth (β = 0.318, SE = 0.071, BC 95% CI = 0.252/0.390), and it also had indirect effects on posttraumatic growth (β = 0.112, SE = 0.023, BC 95%CI = 0.088/0.135) through mediating psychological factors. Coping strategies and the cognitive reappraisal emotion regulation strategy were the main mediating variables, explaining approximately 73.1% of the indirect effect between social support and posttraumatic growth.

Conclusions: Our study suggests that Chinese lung cancer survivors can show relatively high posttraumatic growth. There are many mediating paths between social support and posttraumatic growth. These results may help healthcare professionals to identify the psychosocial factors that may benefit lung cancer patients and develop interventions to promote posttraumatic growth.
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http://dx.doi.org/10.1111/ecc.13450DOI Listing
April 2021

Single-Cell Sequencing Confirms Transcripts and VDJ Rearrangements of Immunoglobulin Genes in Human Podocytes.

Genes (Basel) 2021 Mar 25;12(4). Epub 2021 Mar 25.

Department of Nephrology, Peking University Third Hospital, Beijing 100191, China.

Most glomerular diseases are associated with inflammation caused by deposited pathogenic immunoglobulins (Igs), which are believed to be produced by B cells. However, our previous study indicated that the human podocyte cell line can produce IgG. In this study, we aimed to confirm the transcripts and characterize the repertoires of Igs in primary podocytes at single cell level. First, single-cell RNA sequencing of cell suspensions from "normal" kidney cortexes by a 10xGenomics Chromium system detected Ig transcripts in 7/360 podocytes and Ig gene segments in 106/360 podocytes. Then, we combined nested PCR with Sanger sequencing to detect the transcripts and characterize the repertoires of Igs in 48 single podocytes and found that five classes of Ig heavy chains were amplified in podocytes. Four-hundred and twenty-nine VDJ rearrangement sequences were analyzed; podocyte-derived Igs exhibited classic VDJ rearrangements with nucleotide additions and somatic hypermutations, biased VH1 usage and restricted diversity. Moreover, compared with the podocytes from healthy control that usually expressed one class of Ig and one VDJ pattern, podocytes from patients expressed more classes of Ig, VDJ patterns and somatic hypermutations. These findings suggested that podocytes can express Igs in normal condition and increase diversity in pathological situations.
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http://dx.doi.org/10.3390/genes12040472DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064494PMC
March 2021

Multiple Human Association and Tracking from Egocentric and Complementary Top Views.

IEEE Trans Pattern Anal Mach Intell 2021 Apr 2;PP. Epub 2021 Apr 2.

Crowded scene surveillance can significantly benefit from combining egocentric-view and its complementary top-view cameras. A typical setting is an egocentric-view camera, e.g., a wearable camera on the ground capturing rich local details, and a top-view camera, e.g., a drone-mounted one from high altitude providing a global picture of the scene. To collaboratively analyze such complementary-view videos, an important task is to associate and track multiple people across views and over time, which is challenging and differs from classical human tracking, since we need to not only track multiple subjects in each video, but also identify the same subjects across the two complementary views. This paper formulates it as a constrained mixed integer programming problem, wherein a major challenge is how to effectively measure subjects similarity over time in each video and across two views. Although appearance and motion consistencies well apply to over-time association, they are not good at connecting two highly different complementary views. To this end, we present a spatial distribution based approach to reliable cross-view subject association. We also build a dataset to benchmark this new challenging task. Extensive experiments verify the effectiveness of our method.
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http://dx.doi.org/10.1109/TPAMI.2021.3070562DOI Listing
April 2021

Acoustical modeling of the saxophone mouthpiece as a transfer matrix.

J Acoust Soc Am 2021 Mar;149(3):1901

Computational Acoustic Modeling Laboratory, Center for Interdisciplinary Research in Music Media and Technology, Schulich School of Music, McGill University, Montreal, Quebec H3A 1E3, Canada.

This paper proposes an acoustic model of the saxophone mouthpiece as a transfer matrix (TM). The acoustical influence of the mouthpiece is investigated, and the TM mouthpiece model is compared to previously reported mouthpiece representations, including cylindrical and lumped models. A finite element mouthpiece model is first developed, from which the TM model is derived, and both models are validated by input impedance measurements. The comparison of acoustic properties among different mouthpiece models shows that the TM mouthpiece is more accurate than the other two models, especially in preserving the high-frequency acoustic characteristics. The TM model also produces the best overall tuning of the first several impedance peaks when coupled to a measured saxophone impedance. The internal and radiated sound pressure are synthesized for an alto saxophone connected to different mouthpiece models by jointly modeling the input impedance and the radiation transfer function using recursive parallel filters. Differences are found among mouthpiece models in terms of oscillation thresholds, playing frequencies, spectral centroids, pressure waveforms, and bifurcation delays, which can be partially explained by differences in the tuning and high-frequency characteristics.
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http://dx.doi.org/10.1121/10.0003814DOI Listing
March 2021

Expression of immunoglobulin G in human proximal tubular epithelial cells.

Mol Med Rep 2021 May 24;23(5). Epub 2021 Mar 24.

Department of Nephrology, Peking University Third Hospital, Beijing 100191, P.R. China.

Proximal tubular epithelial cells (PTECs) have innate immune characteristics, and produce proinflammatory factors, chemokines and complement components that drive epithelial‑mesenchymal transition (EMT). Our previous studies revealed that human mesangial cells and podocytes were able to synthesize and secrete immunoglobulin (Ig)A and IgG, respectively. The aim of the present study was to evaluate the expression of Igs in PTECs. Firstly, IgG was detected in the cytoplasm, the cell membrane and the lumen of PTECs in the normal renal cortex by immunohistochemistry. Secondly, Igγ gene transcription and V(D)J recombination were detected in single PTECs by nested PCR and Sanger sequencing. Thirdly, Igγ, Igκ and Igλ were clearly detected in an immortalized PTEC line (HK‑2) by immunostaining and western blotting, in which RP215 (an antibody that predominantly binds to non‑B cell‑derived IgG) was used. In addition, Igγ, Igκ and Igλ gene transcripts, conservative V(D)J recombination in the Igγ variable region, recombination activating gene 1/2 and activation‑induced cytidine deaminase were all detected in HK‑2 cells. These data suggested that PTECs may express IgG in a similar manner to B cells. Furthermore, IgG expression was upregulated by TGF‑β1 and may be involved in EMT.
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http://dx.doi.org/10.3892/mmr.2021.11966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974459PMC
May 2021

ANLN, Regulated by SP2, Promotes Colorectal Carcinoma Cell Proliferation via PI3K/AKT and MAPK Signaling Pathway.

J Invest Surg 2021 Mar 23:1-9. Epub 2021 Mar 23.

Department of General Surgery, The Second Hospital of Anhui Medical University, Hefei, China.

Background: Aberrant expression of Anillin (ANLN) has been shown to function in the development of multiple cancers. However, its effects on colorectal carcinoma (CRC) remain unclear. We aimed to explore the role of ANLN in CRC development.

Methods: By real-time quantitative polymerase chain reaction (RT-qPCR), Western blot, and immunohistochemistry (IHC), we assessed the expression level of ANLN in CRC tissues and cell lines. The role of ANLN in CRC cell proliferation was evaluated by CCK-8 assays, colony formation assays, EdU assays and cell cycle assays. A mouse tumorigenic model was established to evaluate the in vivo function of ANLN.

Results: We found that ANLN was overexpressed in CRC tissues and cell lines. Highly expressed ANLN correlated with tumor size, tumor number, and stage in patients with CRC. Silencing ANLN in CRC cell lines suppressed proliferation both in vitro and in vivo and induced G0/G1 cell cycle arrest. Downregulation of ANLN led to reduced phosphorylated levels of AKT and ERK. However, total AKT protein showed no change. SP2, a critical transcription factor, was implicated in the upregulation of ANLN.

Conclusions: Our study demonstrated that ANLN regulates CRC cell proliferation via the PI3K/AKT and MAPK pathways, indicating that ANLN may represent a novel and effective target for CRC treatment.
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http://dx.doi.org/10.1080/08941939.2020.1850939DOI Listing
March 2021

Synthesis and biological evaluation of disulfides as anticancer agents with thioredoxin inhibition.

Bioorg Chem 2021 May 10;110:104814. Epub 2021 Mar 10.

State Key Laboratory of Applied Organic Chemistry & College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China. Electronic address:

Altered redox homeostasis as a hallmark of cancer cells is exploited by cancer cells for growth and survival. The thioredoxin (Trx), an important regulator in maintaining the intracellular redox homeostasis, is cumulatively recognized as a promising target for the development of anticancer drugs. Herein, we synthesized 72 disulfides and evaluated theirinhibition for Trx and antitumor activity. First, we established an efficient and fast method to screen Trx inhibitors by using the probe NBL-SS that was developed by our group to detect Trx function in living cells. After an initial screening of the Trx inhibitory activity of these compounds, 8 compounds showed significant inhibition activity against Trx. We then evaluated the cytotoxicity of these 8 disulfides, compounds 68 and 69 displayed high cytotoxicity to HeLa cells, but less sensitive to normal cell lines. Next, we performed kinetic studies of both two disulfides, 68 had faster inhibition of Trx than 69. Further studies revealed that 68 led to the accumulation of reactive oxygen species and eventually induced apoptosis of Hela cells via inhibiting Trx. The establishment of a method for screening Trx inhibitors and the discovery of 68 with remarkable Trx inhibition provide support for the development of anticancer candidates with Trx inhibition.
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http://dx.doi.org/10.1016/j.bioorg.2021.104814DOI Listing
May 2021

Clinical importance of ADC in the prediction of I in the treatment for gliomas.

J Cancer 2021 30;12(7):1945-1951. Epub 2021 Jan 30.

Department of the Interventional Medical Center, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.

To determine whether the minimum apparent diffusion coefficient (minADC) value can stratify survival in patients with glioma before I brachytherapy. The study was approved by the Institutional Review Board, and the requirement for informed consent was waived. Twenty-three patients (16 male, 7 female; median age, 48 years) with high-grade glioma (HGG) (n=9) or recurrence after multimodal treatment (n=14) were included in this study. minADC values were obtained before I implantation. Overall survival (OS) and progression-free survival (PFS) were analyzed with Cox proportional hazards regression models and the Kaplan-Meier method with the log-rank test. For I-treated patients, the hazard ratio for OS in patients with ADC≥1.0*10^ mm·sec (high minADC) versus ADC<1.0*10^ mm·sec (low minADC) was 0.220 (95% confidence interval: 0.066, 0.735). The median OS was 12 months for patients with high minADC values and 6.0 months for those with low minADC values, and the differences were significant (p=0.032). The median PFS was 12 months for patients with high minADC values and 4 months for those with low minADC values. Significant differences were found in the long-rank test (p=0.013). The multivariate analysis results showed that minADC pre-I implantation was an independent predictor of OS and PFS in patients receiving I brachytherapy. Pre-I implantation ADC analysis can stratify prognosis in I-treated patients with glioma, which may aid in choosing a suitable therapy for glioma patients.
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http://dx.doi.org/10.7150/jca.50789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974523PMC
January 2021

Computed Tomography- (CT-) Based Virtual Surgery Planning for Spinal Intervertebral Foraminal Assisted Clinical Treatment.

J Healthc Eng 2021 6;2021:5521916. Epub 2021 Mar 6.

Department of Orthopaedics, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, China.

With the development of minimally invasive spine concepts and the introduction of new minimally invasive instruments, minimally invasive spine technology, represented by foraminoscopy, has flourished, and percutaneous foraminoscopy has become one of the most reliable minimally invasive procedures for the treatment of lumbar disc herniation. Percutaneous foraminoscopy is a safe and effective minimally invasive spinal endoscopic surgical technique. It fully protects the paravertebral muscles and soft tissues as well as the posterior column structure of the spine, provides precise treatment of the target nucleus pulposus tissue, with the advantages of less surgical trauma, fewer postoperative complications, and rapid postoperative recovery, and is widely promoted and used in clinical practice. In this paper, we can view the location, morphology, structure, alignment, and adjacency relationships by performing coronary, CT, and diagonal reconstruction along the attachment of the yellow ligaments and performing 3D reconstruction or processing techniques after performing CT scans. This allows clinicians to observe the laminoplasty and the stenosis of the vertebral canal in a more intuitive and overall manner. It has clinical significance for the display of the sublaminar spine as well as the physician's judgment of the disease and the choice of surgery.
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http://dx.doi.org/10.1155/2021/5521916DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960066PMC
March 2021

Accuracy of rapid diagnostic test to diagnose malaria in children under 5 years of age, a meta-analysis.

Diagn Microbiol Infect Dis 2021 Feb 19;100(2):115351. Epub 2021 Feb 19.

Department of Epidemiology and Statistics, School of Public Health, Jilin University, Changchun, Jilin Province, China. Electronic address:

Objective: To assess the accuracy of the rapid diagnostic test for malaria diagnosis in children under 5 years of age.

Methods: As of August 31, 2020, PubMed, Web of Science and Cochrane Library databases had been systematically searched. Relevant data were extracted and meta-analysis was carried out. A random effects model was used for subgroup analysis.

Results: According to the inclusion criteria, a total of 26 studies were included in this meta-analysis. The pooled sensitivity and specificity were 0.92 (95% confidence interval 0.83-0.96) and 0.92 (0.86-0.95), the parasite-specific lactate dehydrogenase-based test were 0.96 (0.85-0.98) and 0.93 (0.86-0.95), the histidine-rich protein 2-based test were 0.94 (0.84-0.98) and 0.86 (0.77-0.91).

Conclusions: This meta-analysis showed that rapid diagnostic test had good accuracy in diagnosing malaria in children under 5 years of age. And the diagnostic performance of parasite-specific lactate dehydrogenase test was better than that of the histidine-rich protein 2 test.
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http://dx.doi.org/10.1016/j.diagmicrobio.2021.115351DOI Listing
February 2021

Dual-color graphene quantum dots and carbon nanoparticles biosensing platform combined with Exonuclease III-assisted signal amplification for simultaneous detection of multiple DNA targets.

Anal Chim Acta 2021 Apr 21;1154:338346. Epub 2021 Feb 21.

State Key Laboratory of Chemical Resource Engineering, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, 100082, China. Electronic address:

Sensitive and simultaneous detection of multiple biomarkers such as target DNA or proteins using biocompatible materials with good analysis performance remains an important challenge. Herein, we successfully developed a signal "off-on" highly sensitive multiplex detection platform based on the combination of dual-color graphene quantum dots (blue GQDs and green GQDs) modified DNA probes with carbon nanoparticles (CNPs), which is a cheap, effective nonfluorescent quencher to simultaneously quench the fluorescence of both GQDs-DNA probes. The Exo III-assisted sequence-independent target recycling and signal amplification strategy was integrated into this sensing platform, which endows it with high sensitivity towards the multiplex detection of targets DNA. The detection limits of 6.6 pM for HIV and 9.5 pM for HBV were achieved respectively, which is about 60-fold lower than that of traditional unamplified homogeneous fluorescent assay methods. Our proposed multiplex detecting platform is advantageous in both respective and simultaneous detection of multiple targets and can also discriminate perfectly matched targets from mismatched targets in both PBS buffer and 1% human serum samples, demonstrating its potential to be a reliable strategy for highly sensitive simultaneous detection of multiple target genes in practical diagnosis applications.
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http://dx.doi.org/10.1016/j.aca.2021.338346DOI Listing
April 2021

Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection.

ACS Nano 2021 04 18;15(4):6340-6351. Epub 2021 Mar 18.

Department of Nephrology, The Key Laboratory for The Prevention and Treatment of Chronic Kidney Disease of Chongqing, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.

The ongoing COVID-19 pandemic worldwide necessitates the development of therapeutics against SARS-CoV-2. ACE2 is the main receptor of SARS-CoV-2 S1 and mediates viral entry into host cells. Herein, membrane nanoparticles (NPs) prepared from ACE2-rich cells were discovered to have potent capacity to block SARS-CoV-2 infection. The membranes of human embryonic kidney-239T cells highly expressing ACE2 were applied to prepare NPs using an extrusion method. The nanomaterials, termed ACE2-NPs, contained 265.1 ng mg ACE2 on the surface and acted as baits to trap S1 in a dose-dependent manner, resulting in reduced recruitment of the viral ligand to HK-2 human renal tubular epithelial cells. Aside from affecting receptor recongnition, S1 translocated to the cytoplasm and induced apoptosis by reducing optic atrophy 1 expression and increasing cytochrome c release, which was also inhibited by ACE2-NPs. Further investigations revealed that ACE2-NPs efficiently suppressed SARS-CoV-2 S pseudovirions entry into host cells and blocked viral infection and . This study characterizes easy-to-produce memrbane nanoantagonists of SARS-CoV-2 that enrich the existing antiviral arsenal and provide possibilities for COVID-19 treatment.
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http://dx.doi.org/10.1021/acsnano.0c06836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009101PMC
April 2021

Toward Covalent Organic Framework Metastructures.

J Am Chem Soc 2021 Apr 16;143(13):5003-5010. Epub 2021 Mar 16.

State Key Lab of Chemical Engineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.

The bottom-up assembly of periodically ordered structures provides a scalable way for producing metastructured materials with exotic optical and mechanical properties. However, direct self-assembly of small molecules into such metastructures beyond the nanoscale remains an unresolved issue. Here we demonstrate that metastructured assemblies of two-dimensional (2D) polymers, specifically 2D covalent organic frameworks (COFs), can be directly synthesized in solution. We applied 2D COF monomer polycondensation to prepare flower-shaped particles consisting of highly crystalline "petals" with sizes larger than 20 μm. The petal comprises periodically arranged COF nanoflake units with tunable lengths of 490-850 nm, thicknesses about 20 nm, interflake spacing around 14 nm, and Hermans orientation factors up to 0.998. Such a metastructure is mechanically robust and remains almost intact even after full pyrolysis at 900 °C. It also demonstrates unique birefringence and polarization-dependent resonances under visible-near-infrared light not observed in its constituents, 2D COF polycrystals, and with well-defined nanopores of 1.8 nm and the high surface area of 1576 m/g. Such metastructured particles with nanopores are well-suited as novel particulate optical devices for collecting and storing information about their surroundings that can be easily read out by polarization imaging with high sensitivity, as demonstrated by their explosive detection and anticounterfeiting applications. Self-assembly of 2D polymers into metastructures may become an important method for developing functional materials with unprecedented properties and extensive applications.
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http://dx.doi.org/10.1021/jacs.0c13090DOI Listing
April 2021