Publications by authors named "Song Peng"

400 Publications

Physical Mechanism of Photoinduced Charge Transfer in One- and Two-Photon Absorption in D-D-π-A Systems.

Materials (Basel) 2021 Jul 14;14(14). Epub 2021 Jul 14.

College of Physics, Liaoning University, Shenyang 110036, China.

The photoinduced charge transfer process of a D-π-A molecule (W1) and three D-D-π-A molecules (WS5-WS7) with triphenylamine as a donor was studied theoretically. D-D-π-A molecules are formed by inserting donors between the triphenylamine and π-linker (π-bridge) on the base of the W1 molecule. The results showed that donor insertion resulted in a red shift in the absorption spectrum, and the absorption intensity increased to a certain extent. A visualization method was used to observe the charge transfer of the four molecules in the process of one- and two-photon absorption (TPA). The local excitation enhanced charge transfer excitation in the TPA process was analyzed and discussed, and the insertion of the thiazolo[5,4-d]thiazole donor showed the largest TPA cross-section. This work contributed to the profound understanding of D-D-π-A molecules and the design of large cross-section TPA molecules.
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http://dx.doi.org/10.3390/ma14143925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306063PMC
July 2021

Functional expression and characterization of a novel aminopeptidase B from in .

Authors:
Peng Song Wei Feng

3 Biotech 2021 Aug 6;11(8):366. Epub 2021 Jul 6.

School of Life Sciences, Liaocheng University, Liaocheng, 252000 China.

A novel aminopeptidase B (APB-AN) was identified from CGMCC 3.1454 for the first time and was cloned and expressed in . The mature enzyme of approximately 100 kDa was purified for characterization. The optimum pH and temperature of the recombinant APB-AN were determined to be 7.0 and 40 °C, respectively. The enzyme was stable below 40 °C and at pH values from 5.0 to 8.0. The and values were determined to be 0.61 mmol/L and 11.45 mmol/L/min, respectively, using Arg-NA as the substrate. APB-AN was inhibited by Cu and Fe and activated by Co and Na. Most metal chelators (Ca, Mg and Mn) and aminopeptidase inhibitors (bestatin and puromycin) suppressed its activity. APB-AN was found to be active towards 13 kinds of amino acid -nitroanilide (NA) substrates:Arg-NA, Lys-NA, Tyr- pNA, Trp-NA, Phe-NA, His-NA, Ala-NA, Met-NA, Leu-NA, Glu-NA, Val-NA, Pro-NA and Ile-NA, and the most preferred N-terminal amino acids were arginine and lysine. APB-AN also hydrolyzed 4 natural proteins: casein, bovine serum albumin, soy protein isolate and water-soluble wheat protein. It is expected that APB-AN has potential food processing applications.
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http://dx.doi.org/10.1007/s13205-021-02915-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257800PMC
August 2021

Systematic Analysis of Survival-Associated Alternative Splicing Signatures in Thyroid Carcinoma.

Front Oncol 2021 23;11:561457. Epub 2021 Jun 23.

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Alternative splicing (AS) is a key mechanism involved in regulating gene expression and is closely related to tumorigenesis. The incidence of thyroid cancer (THCA) has increased during the past decade, and the role of AS in THCA is still unclear. Here, we used TCGA and to generate AS maps in patients with THCA. Univariate analysis revealed 825 AS events related to the survival of THCA. Five prognostic models of AA, AD, AT, ES, and ME events were obtained through lasso and multivariate analyses, and the final prediction model was established by integrating all the AS events in the five prediction models. Kaplan-Meier survival analysis revealed that the overall survival rate of patients in the high-risk group was significantly shorter than that of patients in the low-risk group. The ROC results revealed that the prognostic capabilities of each model at 3, 5, and 8 years were all greater than 0.7, and the final prognostic capabilities of the models were all greater than 0.9. By reviewing other databases and utilizing qPCR, we verified the established THCA gene model. In addition, gene set enrichment analysis showed that abnormal AS events might play key roles in tumor development and progression of THCA by participating in changes in molecular structure, homeostasis of the cell environment and in cell energy. Finally, a splicing correlation network was established to reveal the potential regulatory patterns between the predicted splicing factors and AS event candidates. In summary, AS should be considered an important prognostic indicator of THCA. Our results will help to elucidate the underlying mechanism of AS in the process of THCA tumorigenesis and broaden the prognostic and clinical application of molecular targeted therapy for THCA.
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http://dx.doi.org/10.3389/fonc.2021.561457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261059PMC
June 2021

Activation of UCP2 by anethole trithione suppresses neuroinflammation after intracerebral hemorrhage.

Acta Pharmacol Sin 2021 Jun 28. Epub 2021 Jun 28.

Jiangsu Key Laboratory of Neuropsychiatric Diseases & College of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, China.

Intracerebral hemorrhage (ICH) is a devastating disease, in which neuroinflammation substantially contributes to brain injury. Uncoupling protein 2 (UCP2) is a member of the mitochondrial anion carrier family, which uncouples oxidative phosphorylation from ATP synthesis by facilitating proton leak across the mitochondrial inner membrane. UCP2 has been reported to modulate inflammation. In this study we investigated whether and how UCP2 modulated neuroinflammation through microglia/macrophages following ICH in vitro and in vivo. We used an in vitro neuroinflammation model in murine BV2 microglia to mimic microglial activation following ICH. ICH in vivo model was established in mice through collagenase infusion into the left striatum. ICH mice were treated with anetholetrithione (ADT, 50 mg· kg ·d, ip) or the classical protonophoric uncoupler FCCP (injected into hemorrhagic striatum). We showed that the expression and mitochondrial location of microglial UCP2 were not changed in both in vitro and in vivo ICH models. Knockdown of UCP2 exacerbated neuroinflammation in BV2 microglia and mouse ICH models, suggesting that endogenous UCP2 inhibited neuroinflammation and therefore played a protective role following ICH. ADT enhanced mitochondrial ROS production thus inducing mitochondrial uncoupling and activating UCP2 in microglia. ADT robustly suppressed neuroinflammation, attenuated brain edema and improved neurological deficits following ICH, and these effects were countered by striatal knockdown of UCP2. ADT enhanced AMP-activated protein kinase (AMPK) activation in the hemorrhagic brain, which was abrogated by striatal knockdown of UCP2. Moreover, striatal knockdown of AMPK abolished the suppression of neuroinflammation by ADT following ICH. On the other hand, FCCP-induced mitochondrial uncoupling was independent of UCP2 in microglia; and striatal knockdown of UCP2 did not abrogate the suppression of neuroinflammation by FCCP in ICH mice. In conclusion, the uncoupling activity is essential for suppression of neuroinflammation by UCP2. We prove for the first time the concept that activators of endogenous UCP2 such as anetholetrithione are a new class of uncouplers with translational significance.
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http://dx.doi.org/10.1038/s41401-021-00698-1DOI Listing
June 2021

Osteocrin, a novel myokine, prevents diabetic cardiomyopathy via restoring proteasomal activity.

Cell Death Dis 2021 Jun 16;12(7):624. Epub 2021 Jun 16.

Department of Cardiology, Renmin Hospital of Wuhan University, 430060, Wuhan, China.

Proteasomal activity is compromised in diabetic hearts that contributes to proteotoxic stresses and cardiac dysfunction. Osteocrin (OSTN) acts as a novel exercise-responsive myokine and is implicated in various cardiac diseases. Herein, we aim to investigate the role and underlying molecular basis of OSTN in diabetic cardiomyopathy (DCM). Mice received a single intravenous injection of the cardiotrophic adeno-associated virus serotype 9 to overexpress OSTN in the heart and then were exposed to intraperitoneal injections of streptozotocin (STZ, 50 mg/kg) for consecutive 5 days to generate diabetic models. Neonatal rat cardiomyocytes were isolated and stimulated with high glucose to verify the role of OSTN in vitro. OSTN expression was reduced by protein kinase B/forkhead box O1 dephosphorylation in diabetic hearts, while its overexpression significantly attenuated cardiac injury and dysfunction in mice with STZ treatment. Besides, OSTN incubation prevented, whereas OSTN silence aggravated cardiomyocyte apoptosis and injury upon hyperglycemic stimulation in vitro. Mechanistically, OSTN treatment restored protein kinase G (PKG)-dependent proteasomal function, and PKG or proteasome inhibition abrogated the protective effects of OSTN in vivo and in vitro. Furthermore, OSTN replenishment was sufficient to prevent the progression of pre-established DCM and had synergistic cardioprotection with sildenafil. OSTN protects against DCM via restoring PKG-dependent proteasomal activity and it is a promising therapeutic target to treat DCM.
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http://dx.doi.org/10.1038/s41419-021-03922-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209005PMC
June 2021

A Highly Stable Photodetector Based on a Lead-Free Double Perovskite Operating at Different Temperatures.

J Phys Chem Lett 2021 Jun 11;12(24):5682-5688. Epub 2021 Jun 11.

College of Materials Science and Engineering, Key Laboratory of Advanced Materials of Yunnan Province, Kunming University of Science and Technology, Kunming 650093, Yunnan, China.

In recent years, considerable breakthroughs have been achieved in the explored photodetectors with improved performance and stability. However, such devices suffer from the drifting parameters (photoresponsivity, response time, and specific detectivity) in the case of evident operating temperature changes. Here, a double perovskite CsNaBiCl-based ultraviolet (UV) photodetector is developed free from thermal disturbance, exhibiting a steady photoresponsivity (≈ 67.98 mA/W) and response time (≈ 16.42 ms) within a wide temperature range (from 273 to 333 K). Further studies demonstrate that the stability of the crystal structure endows the superior photodetection capability. This result unambiguously highlights the great potential of such double perovskite CsNaBiCl compound as an environmentally friendly alternative for UV photodetectors.
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http://dx.doi.org/10.1021/acs.jpclett.1c01416DOI Listing
June 2021

Ramulus mori polysaccharide-loaded PLGA nanoparticles and their anti-inflammatory effects in vivo.

Int J Biol Macromol 2021 Jul 1;182:2024-2036. Epub 2021 Jun 1.

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address:

In this study, ramulus mori polysaccharide (RMP) was encapsulated into Poly (lactic-co-glycolicacid) (PLGA) to form PLGA-RMP (PR). The aim of study is to investigate anti-inflammatory effects of PR. The particle size of PR nanoparticles was approximately 205.6 ± 1.86 nm. PR nanoparticles showed significant therapeutic effects on colitis mice model, evidenced by attenuation of the loss of body weight, reduction of the DAI score, and restoration of the colon length. From the histopathological analysis, alleviation of the histopathological damage, less production of IFN-γ and IL-6, and improvement of IL-10 were observed with the treatment of PR. Meanwhile, the treatment of PR not only promoted the expression of ZO-1 and occludin, but also improved the contents of acetate, propionate, and butyrate in the colitis colon. Furthermore, PR extenuated the reduction of the diversity and richness of gut microbiota induced by DSS, and decreased the ratio of Firmicutes to Bacteroidetes while increasing the proportion of Clostridium XIVa, Mucispirillum, and Paraprevotella in the gut microbiota. What's more, PR nanoparticles attenuated the metabolic disorders in the colitis colon induced by DSS. These results indicated that PR nanoparticles could serve as a potent nanomedicine to treat IBD and be used as potential prebiotics.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.200DOI Listing
July 2021

Molecular Targets and Biological Functions of cAMP Signaling in .

Biomolecules 2021 May 3;11(5). Epub 2021 May 3.

School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.

Cyclic AMP (cAMP) is a pivotal signaling molecule existing in almost all living organisms. However, the mechanism of cAMP signaling in plants remains very poorly understood. Here, we employ the engineered activity of soluble adenylate cyclase to induce cellular cAMP elevation in plants and identify 427 cAMP-responsive genes (CRGs) through RNA-seq analysis. Induction of cellular cAMP elevation inhibits seed germination, disturbs phytohormone contents, promotes leaf senescence, impairs ethylene response, and compromises salt stress tolerance and pathogen resistance. A set of 62 transcription factors are among the CRGs, supporting a prominent role of cAMP in transcriptional regulation. The CRGs are significantly overrepresented in the pathways of plant hormone signal transduction, MAPK signaling, and diterpenoid biosynthesis, but they are also implicated in lipid, sugar, K, nitrate signaling, and beyond. Our results provide a basic framework of cAMP signaling for the community to explore. The regulatory roles of cAMP signaling in plant plasticity are discussed.
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http://dx.doi.org/10.3390/biom11050688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147800PMC
May 2021

Malnutrition in Relation with Dietary, Geographical, and Socioeconomic Factors among Older Chinese.

Biomed Environ Sci 2021 May;34(5):337-347

National Institute of Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: Nutrition is closely related to the health of the elderly population. This study aimed to provide a comprehensive picture of the nutrition status of elderly Chinese and its related dietary, geographical, and socioeconomic factors.

Methods: A total of 13,987 ≥ 60-year-old persons from the 2010-2013 Chinese National Nutrition and Health Survey were included to evaluate various aspects of malnutrition, including underweight, overweight or obesity, and micronutrient inadequacy.

Results: Overall, the prevalence of obesity, overweight, and underweight was 12.4%, 34.8%, and 5.7%, respectively, with disparities both geographically and socioeconomically. The prevalence of underweight was higher among the older old (≥ 75 years), rural residents and those with low income, with low education status, and residing in undeveloped West areas. More than 75% of the elderly do not meet the Dietary Reference Intakes for vitamins A, B , B , and E, folate, calcium, selenium, potassium, biotin, and choline, with the prevalence of inadequate intake increasing with age for most nutrients. At the population level, the mean intakes of numerous food groups did not meet the recommendations by the Chinese Dietary Guideline.

Conclusions: Obesity epidemic, inadequacy of micronutrient intake, and high prevalence of underweight and anemia in susceptible older people are the major nutrition challenges for the rapidly aging population in China.
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http://dx.doi.org/10.3967/bes2021.045DOI Listing
May 2021

MicroRNA-27a promotes tumorigenesis in tongue squamous cell carcinoma by enhancing proliferation, migration and suppressing apoptosis.

Eur Arch Otorhinolaryngol 2021 Apr 28. Epub 2021 Apr 28.

Department of Stomatology, the Fourth Hospital of Hebei Medical University, No.12 Jiankang Road, Shijiazhuang, 050000, Hebei province, China.

Background: Tongue squamous cell carcinoma (TSCC) is a major subtype of head and neck squamous cell carcinoma (HNSCC), which is an intractable cancer with a poor prognosis. Studies have shown that microRNAs (miRNAs) play an important role in TSCC biology. However, the expression and functions of miRNAs in TSCC remain unclear.

Methods: The non-coding RNA profiles of TSCC were downloaded from the GEO database. WGCNA (Weighted gene co-expression network analysis) and differential expression miRNA (DE-miRNA) analyses were employed to identify key candidate miRNAs. miRNA expression was detected using RT-qPCR analysis. The target genes of key miRNAs were predicted. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed to explore the potential functions and pathways of key miRNA. miRNA inhibitor was transfected to detect the function of miRNA. The effect of miRNA deregulation on TSCC cell proliferation and apoptosis was investigated using MTS, Annexin V-FITC/PI double staining, and flow cytometry assays.

Results: miR-27a was a key miRNA in TSCC, which was significantly up-regulated in both Cal-27 cells and malignant tissues from the TSCC patients. In addition, functional analysis showed that miR-27a was involved in the regulation of the MAPK, ERBB, and Jak-STAT signaling pathways. Moreover, RHOA and PRKACA were potential target genes of miR-27a, suggesting them as possible mediators of the tumor-promoting effect of miR-27a. Moreover, downregulation of miR-27a inhibited cell proliferation and facilitated cell apoptosis in Cal-27 cells.

Conclusion: Our findings strongly suggest that miR-27a could promote the tumorigenesis and development of TSCC, which makes it a potential new diagnostic marker and therapeutic target for TSCC.
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http://dx.doi.org/10.1007/s00405-021-06837-yDOI Listing
April 2021

Traditional Chinese Medicine Brucea Javanica Oil Enhances the Efficacy of Anlotinib in a Mouse Model of Liver-Metastasis of Small-cell Lung Cancer.

In Vivo 2021 May-Jun;35(3):1437-1441

AntiCancer Inc., San Diego, CA, U.S.A.

Background/aim: Small-cell lung cancer (SCLC) is a recalcitrant disease with liver and other metastasis. The present study evaluated the efficacy of the traditional Chinese medicine Brucea javanica oil (BJO) combined with anlotinib, a multi-tyrosine kinase inhibitor with anti-angiogenic activity, on a nude-mouse model of SCLC liver metastasis.

Materials And Methods: The mouse model was established by injecting NCI-H446 cells (1×10) in Matrigel (20 μl) into the upper liver lobe. All animals were randomized and assigned to three groups: Control (n=8); anlotinib alone (n=8; 3 mg/kg, qd×14+7-day interval with two cycles, oral); anlotinib plus BJO (n=8; 3 mg/kg anlotinib qd×14+7-day interval with two cycles, orally; BJO: 1 g/kg, qd×6 weeks, orally). Body weight was determined every week. Six weeks after initial treatment, tumors were collected for analysis of angiogenesis using immunohistochemistry.

Results: The combination of anlotinib and BJO significantly inhibited growth of SCLC liver metastases and angiogenesis more than anlotinib monotherapy (p=0.043). In addition, BJO alleviated body-weight loss associated with anlotinib therapy, including general mouse condition.

Conclusion: The results of the present study indicate that the combination of anlotinib with BJO is promisingly active against liver metastases of SCLC, and has clinical potential.
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http://dx.doi.org/10.21873/invivo.12395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193324PMC
June 2021

The role of chest CT quantitative pulmonary inflammatory index in the evaluation of the course and treatment outcome of COVID-19 pneumonia.

Sci Rep 2021 04 8;11(1):7752. Epub 2021 Apr 8.

Department of Radiology, First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing, 400016, China.

To explore the clinical application value of chest CT quantitative pulmonary inflammation index (PII) in the evaluation of the course and treatment outcome of COVID-19 pneumonia. One hundred and eighteen patients with COVID-19 pneumonia diagnosed by RT-PCR were analyzed retrospectively. The correlation between chest CT PII, clinical symptoms and laboratory examinations during the entire hospitalization period was compared. The average age of the patients was 46.0 ± 15 (range: 1-74) years. Of the 118 patients, 62 are male (52.5%) and 56 are female (47.5%). Among them, 116 patients recovered and were discharged, 2 patients died, and the median length of hospital stay was 22 (range: 9-41) days. On admission, 76.3% of the patients presented with fever, and the laboratory studies showed a decrease in lymphocyte (LYM) count and an increase in lactate dehydrogenase (LDH) levels, C-reactive protein (CRP) levels, and erythrocyte sedimentation rate (ESR). Within the studies' chest CTs, the median number of involved lung lobes was 4 (range: 0-5) and the median number of involved lung segments was 9 (range 0-20). The left lower lobe and the right lower lobe were the most likely areas to be involved (89.0% and 83.9%), and 84.7% of the patients had inflammatory changes in both lungs. The main manifestations on chest CT were ground glass opacities (31.4%), ground glass opacities and consolidation (20.3%), ground glass opacities and reticular patterns (32.2%), mixed type (13.6%), and white lungs (1.7%); common accompanying signs included linear opacities (55.9%), air bronchograms (46.6%), thick small vessel shadows (36.4%), and pleural hypertrophy (13.6%). The chest CT at discharge showed complete absorption of lesions in 19 cases (16.1%), but not in the remaining 99 cases. Lesions remained in a median of 3 lung lobes (range: 0-5). Residual lesions remained in a median of 5 lung segments (range: 0-20). The residual lesions mainly presented as ground glass opacities (61.0%), and the main accompanying sign was linear opacities (59.3%). Based on chest CT, the median maximum PII of lungs was 30.0% (range: 0-97.5%), and the median PII after discharge in the patients excluding the two deaths was 12.5% (range: 0-53.0%). PII was significantly negatively correlated with the LYM count and significantly positively correlated with body temperature, LDH, CRP, and ESR. There was no significant correlation between the PII and the white blood cell count, but the grade of PII correlated well with the clinical classification. PII can be used to monitor the severity and the treatment outcome of COVID-19 pneumonia, provide help for clinical classification, assist in treatment plan adjustments and aid assessments for discharge.
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http://dx.doi.org/10.1038/s41598-021-87430-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032697PMC
April 2021

Osthole Attenuates Macrophage Activation in Experimental Asthma by Inhibitingthe NF-ĸB/MIF Signaling Pathway.

Front Pharmacol 2021 22;12:572463. Epub 2021 Mar 22.

Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Guilin Medical University, Guilin, China.

Inhibition of activated macrophages is an alternative therapeutic strategy for asthma. We investigated whether a coumarin compound, osthole, isolated from (L.) Cuss, alleviated macrophage activation and . Osthole could reduce expression of a marker of activated macrophages, cluster of differentiation (CD)206, in an ovalbumin-challenge model of asthma in mice. Osthole could also inhibit infiltration of inflammatory cells, collagen deposition and production of proinflammatory cytokines [interleukin (IL)-1β, tumor necrosis factor-ɑ, macrophage migration inhibitory factor (MIF)] in asthmatic mice. , expression of phosphorylated-IĸBɑ, MIF and M2 cytokines (Ym-1, Fizz-1, arginase-1) in IL-4-induced macrophages decreased upon exposure to the NF-ĸB inhibitor MG-132. In our short hairpin (sh)RNA-MIF-knockdown model, reduced expression of M2 cytokines was detected in the IL-4 + shRNA-MIF group. Osthole could attenuate the proliferation and migration of an IL-4-induced rat alveolar macrophages line (NR8383). Osthole could reduce IL-4-induced translocation of nuclear factor-kappa B (NF-ĸB) in NR8383 cells. Collectively, our results suggest that osthole ameliorates macrophage activation in asthma by suppressing the NF-ĸB/MIF signaling pathway, and might be a potential agent for treating asthma.
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http://dx.doi.org/10.3389/fphar.2021.572463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020258PMC
March 2021

Carboxypeptidase A4 negatively correlates with p53 expression and regulates the stemness of breast cancer cells.

Int J Med Sci 2021 18;18(8):1753-1759. Epub 2021 Feb 18.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, P.R. China.

Triple-negative breast cancer (TNBC) is an aggressive cancer subtype lacking effective treatment options, and p53 is the most frequently mutated or deleted gene. Carboxypeptidase A4 (CPA4) is an extracellular metallocarboxypeptidase, which was closely associated with aggressiveness. Although a recent study indicated that CPA4 could induce epithelial‑mesenchymal transition in breast cancer cells, no studies investigated its stemness-related function and the correlation between CPA4 and p53 in TNBC. In this study, we aimed to investigate the CPA4 levels in breast cancer tissues and analyze its association with p53, and study its roles in cancer stemness maintenance. CPA4 mRNA level and its prognostic value were analyzed by using online database UALCAN (http://ualcan.path.uab.edu) and Kaplan-Meier plotter (www.kmplot.com), respectively. The expression of CPA4, p53 and ALDH1A1 in breast cancer and adjacent normal tissues were evaluated by IHC using the corresponding primary antibodies on a commercial tissue array (Shanghai Biochip Co., Ltd., Shanghai, China). siRNA knockdown was used to study the function of proliferation, colony formation assay and sphere formation in serum-free medium. Analysis of the UALCAN datasets identified that CPA4 mRNA levels were elevated in TNBC, especially in the TP53-mutant subgroup. Furthermore, high levels of CPA4 mRNA were significantly associated with unfavourable overall survival OS in breast cancer patients. Immunohistochemistical analysis demonstrated that CPA4 levels were elevated in 32.1% of breast cancer samples (45/140), and the positive rates of ALDH1A1 and p53 in the breast cancer tissues were 25% (35/140) and 50% (70/140), respectively. Statistical analysis revealed high levels of CPA4 was significantly associated with TNBC phenotype. Correlation analysis indicated that CPA4 over-expression was positively associated with ALDH1A1 (P<0.01) and negatively correlated with p53 (P<0.05). In Kaplan-Meier survival analysis, either high CPA4 or ALDH1A1 levels was significantly correlated with poor survival in breast cancer patients. Functional studies demonstrated that down-regulation of CPA4 significantly inhibited TNBC cell proliferation, colony-formation assays in soft agar and sphere formation in serum-free medium. This study demonstrated for the first time that CPA4 was negatively correlates with p53 expression and inhibition of CPA4 could reduce the number of breast cancer cells with stemness property. It might be a potential target for the TNBC treatment.
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http://dx.doi.org/10.7150/ijms.54954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7976593PMC
February 2021

Molecular Characterization and Immunological Evaluation of Truncated Rhoptry Neck Protein 2 as a Vaccine Candidate.

Front Immunol 2021 24;12:616343. Epub 2021 Feb 24.

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China.

is a protozoan that infects red blood cells. Babesiosis is becoming a new global threat impacting human health. Rhoptry neck proteins (RONs) are proteins located at the neck of the rhoptry and studies indicate that these proteins play an important role in the process of red blood cell invasion. In the present study, we report on the bioinformatic analysis, cloning, and recombinant gene expression of two truncated rhoptry neck proteins 2 (BmRON2), as well as their potential for incorporation in a candidate vaccine for babesiosis. Western blot and immunofluorescence antibody (IFA) assays were performed to detect the presence of specific antibodies against BmRON2 in infected mice and the localization of N-BmRON2 in parasites. experiments were carried out to investigate the role of BmRON2 proteins during the invasion process and experiments to investigate immunoprotection. Homologous sequence alignment and molecular phylogenetic analysis indicated that BmRON2 showed similarities with RON2 proteins of other species. We expressed the truncated N-terminal (33-336 aa, designated rN-BmRON2) and C-terminal (915-1171 aa, designated rC-BmRON2) fragments of the BmRON2 protein, with molecular weights of 70 and 29 kDa, respectively. Western blot assays showed that the native BmRON2 protein is approximately 170 kDa, and that rN-BmRON2 was recognized by serum of mice experimentally infected with Immunofluorescence analysis indicated that the BmRON2 protein was located at the apical end of merozoites, at the opposite end of the nucleus. red blood cell invasion inhibition studies with rBmRON2 proteins showed that relative invasion rate of rN-BmRON2 and rC-BmRON2 group is 45 and 56%, respectively. Analysis of the host immune response after immunization and infection showed that both rN-BmRON2 and rC-BmRON2 enhanced the immune response, but that rN-BmRON2 conferred better protection than rC-BmRON2. In conclusion, our results indicate that truncated rhoptry neck protein 2, especially its N-terminal fragment (rN-BmRON2), plays an important role in the invasion of host red blood cells, confers immune protection, and shows good potential as a candidate vaccine against babesiosis.
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http://dx.doi.org/10.3389/fimmu.2021.616343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943735PMC
July 2021

Continuous artificial light at night exacerbates diisononyl phthalate-induced learning and memory impairment in mice: Toxicological evidence.

Food Chem Toxicol 2021 May 9;151:112102. Epub 2021 Mar 9.

Laboratory of Environment-immunological and neurological diseases, Research Center of Basic Medical Sciences, Hubei University of Science and Technology, Xianning, 437100, PR China; Xianning Engineering Research Center for Healthy Environment, Xianning, 437100, PR China. Electronic address:

Previously, we reported that exposure to diisononyl phthalate (DINP) resulted in cognitive deficits and anxiety in mice (https://doi.org/10.1038/srep14676). Artificial light at night (ALAN) is now recognized as being a potential threat to human health. However, toxicological evidence concerning exposure to a combination of ALAN and DINP in vivo is limited. To this end, mice were orally exposed to different concentrations of DINP for 28 consecutive days, and ALAN (intensity 150 lux, every night for 12 h). The results showed that oxidative stress levels increased with increasing DINP exposure concentrations, which triggered apoptosis (Bcl-2 levels decreased, Bax levels increased), resulting in nerve cell damage and a decline in the learning and memory abilities of mice. The combined effects of ALAN and DINP exposure on the learning ability and memory of mice are more serious than for DINP exposure alone. The antioxidant vitamin E was shown to have a certain antagonistic effect on the oxidative damage caused by ALAN and DINP exposure. These results highlight a previously unknown relationship between exposure to ALAN and DINP-induced learning and memory impairment, and provide evidence that ALAN may be exacerbating the effects of DINP.
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http://dx.doi.org/10.1016/j.fct.2021.112102DOI Listing
May 2021

Ag-ZnO Nanocomposites Are Used for SERS Substrates and Promote the Coupling Reaction of PATP.

Materials (Basel) 2021 Feb 15;14(4). Epub 2021 Feb 15.

College of Chemistry, Liaoning University, Shenyang 110036, China.

Noble metal-semiconductor nanocomposites have received extensive attention in Surface Enhanced Raman Scattering (SERS) due to their unique properties. In this paper, the Ag-ZnO nanocomposites are prepared by hydrothermal growth and simple chemical reduction immersion. The synthesized nanocomposite material simultaneously integrates the individual enhancement effects of the two materials in the SERS, such as the electromagnetic enhancement of silver nanoparticles and the chemical enhancement of ZnO semiconductor materials. Using this substrate, Rhodamine 6G molecules with a concentration as low as 10 M can be detected, and the coupling reaction of PATP can be effectively promoted. The nanocomposite materials prepared by selecting appropriate semiconductor materials and metal materials combined, could be potentially applied, as SERS substrates, in certain catalytic reactions.
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http://dx.doi.org/10.3390/ma14040922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919486PMC
February 2021

Antioxidant Enzymatic Activity and Osmotic Adjustment as Components of the Drought Tolerance Mechanism in .

Plants (Basel) 2021 Feb 25;10(3). Epub 2021 Feb 25.

Beijing Research Center of Intelligent Equipment for Agriculture, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.

Drought stress is a major environmental constraint for plant growth. Climate-change-driven increases in ambient temperatures resulted in reduced or unevenly distributed rainfalls, leading to increased soil drought. C. A. Mey is a typical drought-tolerant sedge, but few reports have examined the mechanisms conferring its tolerant traits. In the present study, the drought responses of were assessed by quantifying activity of antioxidant enzymes in its leaf and root tissues and evaluating the relative contribution of organic and inorganic osmolyte in plant osmotic adjustment, linking it with the patterns of the ion acquisition by roots. Two levels of stress-mild (MD) and severe (SD) drought treatments-were used, followed by re-watering. Drought stress caused reduction in a relative water content and chlorophyll content of leaves; this was accompanied by an increase in the hydrogen peroxide (HO) and superoxide (O) contents in leaves and roots. Under MD stress, the activities of catalase (CAT), peroxidase (POD), and glutathione peroxidase (GPX) increased in leaves, whereas, in roots, only CAT and POD activities increased. SD stress led to an increase in the activities of CAT, POD, superoxide dismutase (SOD), and GPX in both tissues. The levels of proline, soluble sugars, and soluble proteins in the leaves also increased. Under both MD and SD stress conditions, increased K, Na, and Cl uptake by plant roots, which resulted in an increased K, Na, and Cl concentrations in leaves and roots. This reliance on inorganic osmolytes enables a cost-efficient osmotic adjustment in . Overall, this study revealed that was able to survive arid environments due to an efficient operation of its ROS-scavenging systems and osmotic adjustment mechanisms.
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http://dx.doi.org/10.3390/plants10030436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996351PMC
February 2021

Elevated Heterogeneous Nuclear Ribonucleoprotein C Expression Correlates With Poor Prognosis in Patients With Surgically Resected Lung Adenocarcinoma.

Front Oncol 2020 25;10:598437. Epub 2021 Jan 25.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Lung adenocarcinoma (LUAD), as the most common histological subtype of lung cancer, is a high-grade malignancy and a leading cause of cancer-related death globally. Identification of biomarkers with prognostic value is of great significance for the diagnosis and treatment of LUAD. Heterogeneous nuclear ribonucleoprotein C (HNRNPC) is an RNA-binding protein "reader" of N6-methyladenosine (mA) methylation, and is related to the progression of various cancers; however, its role in LUAD is unclear. The aims of this study aims were to study the expression and prognostic value of HNRNPC in LUAD.

Methods: The Oncomine database and gene expression profiling interactive analysis (GEPIA) were used for preliminary exploration of HNRNPC expression and prognostic value in LUAD. LUAD cases from The Cancer Genome Atlas (TCGA) (n = 416) and the Kaplan-Meier plotter database (n = 720) were extracted to study the differential expression and prognostic value of HNRNPC. HNRNPC expression in the National Cancer Center of China (NCC) cohort was analyzed by immunohistochemical staining, and the relationship between HNRNPC expression and survival rate evaluated using the Kaplan-Meier method and log-rank test. Univariate and multivariate Cox regression analyses were used to identify independent prognostic factors. Several pathways that were significantly enriched in the HNRNPC high expression group were identified by Gene Set Enrichment Analysis (GSEA).

Results: Five data sets from the Oncomine and GEPIA databases all supported that HNRNPC expression is significantly higher in LUAD than in normal lung tissue. In TCGA cohort, HNRNPC was highly expressed in LUAD tissues and significantly related to age, sex, smoking history, ethnicity, lymph node metastasis, and TNM staging ( < 0.001). High HNRNPC expression was significantly correlated with poor prognosis in the three cohorts (NCC, TCGA, and K-M plotter) ( < 0.05). Multivariate Cox regression analysis showed that HNRNPC expression was an independent prognostic factor in both TCGA and NCC cohorts ( < 0.05). Further, 10 significantly enriched pathways were identified from TCGA data and 118 lung cancer cell lines in CCLE, respectively.

Conclusions: High HNRNPC expression is significantly related to poor overall survival in patients with LUAD, suggesting that HNRNPC may be a cancer-promoting factor and a potential prognostic biomarker in LUAD.
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http://dx.doi.org/10.3389/fonc.2020.598437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868529PMC
January 2021

Association between Baseline SBP/DBP and All-Cause Mortality in Residents of Shanxi, China: A Population-based Cohort Study from 2002 to 2015.

Biomed Environ Sci 2021 Jan;34(1):1-8

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To investigate the association between blood pressure and all-cause mortality in Shanxi, China.

Methods: The '2002 China Nutrition and Health Survey' baseline data in Shanxi province was used. A retrospective investigation was performed in 2015. The effects of SBP and DBP on the all-cause mortality were analyzed using the Cox regression model. The hazard ratio ( ) and 95% confidence interval ( ) were estimated by the sex and age groups.

Results: The follow-up rate was 76.52% over 13 years, while the cumulative mortality rate for all participants was 917.12/100,000 person-years. The mortality rose with an increasing SBP ( = 270.537, < 0.001) or DBP level ( = 57.240, < 0.001). After adjustment for the confounding factors, a significant association between mortality and high SBP (≥ 160 mmHg) and high DBP (≥ 100 mmHg), with adjusted ranging from 1.405- to 2.179-fold for SBP and 1.550- to 2.854-fold for DBP, was noted. Significant HRs for most DBP subgroups were found in > 60-year-old participants. Males with DBP ≥ 100 mmHg had a significantly higher mortality, with an (95% ) of 2.715 (1.377-5.351).

Conclusion: Adults with SBP > 160 mmHg and DBP > 100 mmHg had a higher mortality risk. Sex and age difference was noted in both DBP and mortality risk.
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http://dx.doi.org/10.3967/bes2021.002DOI Listing
January 2021

Prognostic Impact of Expression in Patients with Surgically Resected Lung Adenocarcinoma.

DNA Cell Biol 2021 Feb 25;40(2):316-331. Epub 2021 Jan 25.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

Lung adenocarcinoma (LUAD) acts as the representative histological subtype regarding lung cancer, and its mortality and morbidity remain high. Therefore, more therapeutic targets are needed. Insulin-like growth factor 2 messenger RNA-binding proteins (IGF2BPs), a class of RNA-binding proteins, including , , and , are considered to be the "reader" of N6-methyladenosine (m6A) methylation and remarkably affects cancer occurrence and development. Studies have shown that has prognostic potential in multiple public databases compared with other members of the IGF2BPs family. This research aims to use the expression data of in multiple cohorts to explore the expression as well as prognostic significance of in LUAD. Based on the six datasets from the Oncomine database, LUAD tissue presented obviously higher expression relative to normal tissue. In the three cohorts of The Cancer Genome Atlas (TCGA), Gene expression profiling interactive analysis, and Gene Expression Omnibus, was abnormally highly expressed in LUAD, and it could lead to a weaker prognosis ( < 0.05). The cohort from National Cancer Center of China (NCC) verified the prognostic value of , and the high expression could be remarkably related to gender, tumor length, differentiation, and T stage ( < 0.05). Cox regression analysis of TCGA and NCC cohorts consistently showed that expression could serve for independently predicting the prognosis of LUAD patients ( < 0.05). Ten hallmark pathways with significant enrichment were identified. is abnormally highly expressed in LUAD tissue, and can lead to worse overall survival. In summary, may be an oncogene and potential prognostic biomarker of LUAD.
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http://dx.doi.org/10.1089/dna.2020.6136DOI Listing
February 2021

Endothelial ERG alleviates cardiac fibrosis via blocking endothelin-1-dependent paracrine mechanism.

Cell Biol Toxicol 2021 Jan 20. Epub 2021 Jan 20.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.

Cardiac endothelium communicates closely with adjacent cardiac cells by multiple cytokines and plays critical roles in regulating fibroblasts proliferation, activation, and collagen synthesis during cardiac fibrosis. E26 transformation-specific (ETS)-related gene (ERG) belongs to the ETS transcriptional factor family and is required for endothelial cells (ECs) homeostasis and cardiac development. This study aims at investigating the potential role and molecular basis of ERG in fibrotic remodeling within the adult heart. We observed that ERG was abundant in murine hearts, especially in cardiac ECs, but decreased during cardiac fibrosis. ERG knockdown within murine hearts caused spontaneously cardiac fibrosis and dysfunction, accompanied by the activation of multiple Smad-dependent and independent pathways. However, the direct silence of ERG in cardiac fibroblasts did not affect the expression of fibrotic markers. Intriguingly, ERG knockdown in human umbilical vein endothelial cells (HUVECs) promoted the secretion of endothelin-1 (ET-1), which subsequently accelerated the proliferation, phenotypic transition, and collagen synthesis of cardiac fibroblasts in a paracrine manner. Suppressing ET-1 with either a neutralizing antibody or a receptor blocker abolished ERG knockdown-mediated deleterious effect in vivo and in vitro. This pro-fibrotic effect was also negated by RGD (Arg-Gly-Asp)-peptide magnetic nanoparticles target delivery of ET-1 small interfering RNA to ECs in mice. More importantly, we proved that endothelial ERG overexpression notably prevented pressure overload-induced cardiac fibrosis. Collectively, endothelial ERG alleviates cardiac fibrosis via blocking ET-1-dependent paracrine mechanism and it functions as a candidate for treating cardiac fibrosis. • ERG is abundant in murine hearts, especially in cardiac ECs, but decreased during fibrotic remodeling. • ERG knockdown causes spontaneously cardiac fibrosis and dysfunction. • ERG silence in HUVECs promotes the secretion of endothelin-1, which in turn activates cardiac fibroblasts in a paracrine manner. • Endothelial ERG overexpression prevents pressure overload-induced cardiac fibrosis.
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http://dx.doi.org/10.1007/s10565-021-09581-5DOI Listing
January 2021

Effect of Polymerization on the Charge-Transfer Mechanism in the One (Two)-Photon Absorption Process of D-A-Type Triphenylamine Derivatives.

J Phys Chem A 2021 Jan 12;125(3):777-794. Epub 2021 Jan 12.

Department of Physics, Liaoning University, Shenyang, 110036 Liaoning, China.

To investigate the effect of polymerization ( = 1, 2, 3, and 4) on the charge-transfer (CT) mechanisms in the one (two)-photon absorption (OPA and TPA) process of D-A-type triphenylamine derivatives, charge density difference is used to graphically represent the CT characteristics. A transition density matrix is utilized to reveal the direction of CT on different groups quantitatively. With the increasing, electrons are mainly transferred between the groups in the middle position of the molecular chain during OPA and TPA processes. Simulated results show that the energy gap and excitation energy have a good linear relationship with the reciprocal of the polymerization degree. Importantly, the polymerization effect can effectively increase the electronic transmission capability, TPA performance, and second hyperpolarizability. Besides, the simplified sum over state model reveals the variation factor of the TPA cross-section and the second static hyperpolarizability. The McRae formula and Bakhshiev formula are used to estimate the difference of dipole moments, which is an important parameter of the second hyperpolarizability. The comprehensive analysis of the nonlinear optical (NLO) parameters of triphenylamine derivatives can provide some significant guidance for molecular design and improve the NLO performance of D-A molecular materials. Also, the thermodynamic parameters can provide some theoretical supports for solving practical problems.
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http://dx.doi.org/10.1021/acs.jpca.0c09309DOI Listing
January 2021

A review of physiological and cellular mechanisms underlying fibrotic postoperative adhesion.

Int J Biol Sci 2021 1;17(1):298-306. Epub 2021 Jan 1.

Department of Gastrointestinal Surgery, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School.

Postoperative adhesions (PA) are fibrotic tissues that are the most common driver of long-term morbidity after abdominal and pelvic surgery. The optimal drug or material to prevent adhesion formation has not yet been discovered. Comprehensive understanding of cellular and molecular mechanisms of adhesion process stimulates the design of future anti-adhesive strategies. Recently, disruption of peritoneal mesothelial cells were suggested as the 'motor' of PA formation, followed by a cascade of events (coagulation, inflammation, fibrinolysis) and influx of various immune cells, ultimately leading to a fibrous exudate. We showed that a variety of immune cells were recruited into adhesive peritoneal tissues in patients with small bowel obstruction caused by PA. The interactions among various types of immune cells contribute to PA development following peritoneal trauma. Our review focuses on the specific role of different immune cells in cellular and humoral mechanisms underpinning adhesion development.
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http://dx.doi.org/10.7150/ijbs.54403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757036PMC
January 2021

[Characteristics and Ecological Risk Assessment of POPs Pollution in Sediments of Xiaoxingkai Lake in the Northeast China].

Huan Jing Ke Xue 2021 Jan;42(1):147-158

National Engineering Laboratory of Lake Water Pollution Control and Ecological Restoration Technology, Chinese Resarch Academy of Environmental Sciences, Beijing 100012, China.

The pollution characteristics of polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs), and phthalate esters (PAEs) in the surface sediments of Xiaoxingkai Lake were analyzed by GC-MS, and the main sources and biological toxicity risks of the pollutants were discussed. The results show that:① The content of PAHs in the sediments of xiaoxingkai lake ranged from 82.1 to 534.6 ng·g, and the concentration of PAHs in the northwestern port of the lake was higher. The content of OCPs and PAEs in the sediments ranged from 4.8 to 50.4 ng·g and 33.3 to 401.6 ng·g, respectively. The concentration was higher in the southeastern lakes. ② PAHs in the sediments were dominated by 3-5-ring compounds (accounting for more than 85%), which were mainly combustion sources, among which the combustion of coal and firewood contributed 47%, the combustion of gasoline and diesel contributed 39%, and the oil product leakage contributed 14%. The OCPs were mainly HCHs (78%) from the use of new lindane and the input of a small amount of industrial HCHs. The PAEs were mainly dibutyl phthalate (DBP) and diethyl phthalate (2-ethyl hexyl) ester (DEHP; 94%), which were mainly derived from household garbage and common human articles. ③ Compared with other lakes in China, PAHs and PAEs in the sediments of Xiaoxingkai Lake are at a low pollution level, and there is no ecological risk at present, but some OCPs at some points present a moderate ecological risk.
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http://dx.doi.org/10.13227/j.hjkx.202005131DOI Listing
January 2021

The VHL/HIF Axis in the Development and Treatment of Pheochromocytoma/Paraganglioma.

Front Endocrinol (Lausanne) 2020 24;11:586857. Epub 2020 Nov 24.

Department of Urology, Daping Hospital, Army Medical University, Chongqing, China.

Pheochromocytomas and paragangliomas (PPGLs) are rare neuroendocrine tumors originating from chromaffin cells in the adrenal medulla (PCCs) or extra-adrenal sympathetic or parasympathetic paraganglia (PGLs). About 40% of PPGLs result from germline mutations and therefore they are highly inheritable. Although dysfunction of any one of a panel of more than 20 genes can lead to PPGLs, mutations in genes involved in the VHL/HIF axis including , , , and are more frequently found in PPGLs. Multiple lines of evidence indicate that pseudohypoxia plays a crucial role in the tumorigenesis of PPGLs, and therefore PPGLs are also known as metabolic diseases. However, the interplay between VHL/HIF-mediated pseudohypoxia and metabolic disorder in PPGLs cells is not well-defined. In this review, we will first discuss the VHL/HIF axis and genetic alterations in this axis. Then, we will dissect the underlying mechanisms in VHL/HIF axis-driven PPGL pathogenesis, with special attention paid to the interplay between the VHL/HIF axis and cancer cell metabolism. Finally, we will summarize the currently available compounds/drugs targeting this axis which could be potentially used as PPGLs treatment, as well as their underlying pharmacological mechanisms. The overall goal of this review is to better understand the role of VHL/HIF axis in PPGLs development, to establish more accurate tools in PPGLs diagnosis, and to pave the road toward efficacious therapeutics against metastatic PPGLs.
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http://dx.doi.org/10.3389/fendo.2020.586857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732471PMC
May 2021

Multi-Level Effects of Humble Leadership on Employees' Work Well-Being: The Roles of Psychological Safety and Error Management Climate.

Front Psychol 2020 11;11:571840. Epub 2020 Nov 11.

College of Business and Economics, University of Australian National, Canberra, ACT, Australia.

Employees' work well-being (WWB) is vital to employees' performance and organizations' sustainable development. This study aims to explore the role of psychological safety and error management climate (EMC) between humble leadership and WWB in Chinese organizations. Drawing upon social information processing theory, a multi-level study was conducted to test the underlying mechanisms between humble leadership and employees' WWB. A time-lagged data of 221 team members was collected from 12 small and medium sized companies in China. Results showed that team-level humble leadership was positively related to WWB. Psychological safety and EMC both played a partial mediating role linking humble leadership and WWB. EMC positively moderated the relationship between humble leadership and psychological safety. This paper contributes to revealing the multi-level effects of humble leadership on work well-being. These findings also provide some important implications for managerial practices.
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http://dx.doi.org/10.3389/fpsyg.2020.571840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685992PMC
November 2020

Survival Nomogram for Stage IB Non-Small-Cell Lung Cancer Patients, Based on the SEER Database and an External Validation Cohort.

Ann Surg Oncol 2021 Jul 28;28(7):3941-3950. Epub 2020 Nov 28.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objective: This study aimed to construct a nomogram to effectively predict the overall survival (OS) of patients with stage IB non-small-cell lung cancer (NSCLC).

Methods: In total, 5513 patients with stage IB NSCLC were extracted from the Surveillance, Epidemiology, and End Results (SEER) database and used as the training cohort. We enrolled 440 patients from the Cancer Hospital, Chinese Academy of Medical Sciences, for the external validation cohort. A nomogram was constructed based on the risk factors affecting prognosis using a Cox proportional hazards regression model. The discrimination and calibration of the nomogram were evaluated by C-indexes and calibration curves.

Results: Six independent risk factors for OS were identified and integrated into the nomogram. The discrimination of the nomogram revealed good prognostic accuracy and clinical applicability as indicated by C-index values of 0.637 (95% CI 0.634-0.641) and 0.667 (95% CI 0.656-0.678) for the training cohort and the external validation cohort, respectively. Additionally, the patients were divided into two groups according to risk (sum-score > 185), and significant differences in OS were observed between the high-risk and low-risk groups in the training and external validation cohorts (P < 0.001). Finally, chemotherapy was significantly associated with OS in patients with differentiation grades II-IV (P = 0.004) and patients with adenocarcinoma (P = 0.005).

Conclusion: This nomogram provides a convenient and reliable tool for individual evaluations and clinical decision-making for patients with stage IB NSCLC; among these patients, those with differentiation grades II-IV or adenocarcinoma could benefit from chemotherapy.
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http://dx.doi.org/10.1245/s10434-020-09362-0DOI Listing
July 2021

Overexpression of microRNA-124-5p sensitizes non-small cell lung cancer cells to treatment with 5-fluorouracil via AEG-1 regulation.

Oncol Lett 2021 Jan 3;21(1). Epub 2020 Nov 3.

Department of Respiration, The Third People's Hospital of Linyi City, Linyi, Shandong 371312, P.R. China.

Chemotherapeutic resistance represents a major obstacle for the treatment of patients with non-small cell lung cancer (NSCLC); however, the associated molecular mechanisms underpinning the development of resistance remain poorly characterized. In the current study, 5-fluorouracil (5-FU)-resistant A549 cells (A549/5-FU) were generated from A549 cells. Reverse transcription-quantitative PCR and western blotting were used to detect microRNA(miR)-124-5p and astrocyte elevated gene 1 (AEG-1) expression levels in cells and tumor tissues. In addition, the cytotoxic effect of 5-FU on the cells was determined using the Cell Counting Kit-8 assay, and the Dual-luciferase reporter assay was used to validate AEG-1 as a target gene of miR-124-5p. Transfection with a miR-124-5p mimic enhanced inhibition of cell viability induced by 5-FU in A549/5-FU cells, whereas miR-124-5p inhibitor transfection partially reversed 5-FU-induced cell viability inhibition in A549 and H1299 cells. A decrease in miR-124-5p expression level was observed in A549/5-FU cells compared with the parental A549 cells. Furthermore, AEG-1 was predicted as a target gene of miR-124-5p, and its expression was increased in A549/5-FU cells compared with A549 cells. Additionally, the upregulation of miR-124-5p was associated with lower expression levels of AEG-1 in A549/5-FU cells, compared with parental A549 cells. Moreover, the Dual-luciferase reporter assay confirmed the ability of miR-124-5p to bind directly to the 3'-untranslated region of AEG-1 mRNA. Notably, the overexpression of AEG-1 reversed the ability of the miR-124-5p mimic to increase the sensitivity of A549/5-FU cells to 5-FU treatment. Additionally, a significant negative correlation between miR-124-5p expression and AEG-1 mRNA levels was detected in 40 pairs of NSCLC tissues and their corresponding adjacent paracancerous tissues. The results of the present study indicated that miR-124-5p may regulate the chemotherapeutic sensitivity of NSCLC cells, and may therefore represent a promising biomarker or therapeutic target for patients with NSCLC.
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http://dx.doi.org/10.3892/ol.2020.12266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7681228PMC
January 2021

Computational Design of Self-actuated Deformable Solids via Shape Memory Material.

IEEE Trans Vis Comput Graph 2020 Nov 23;PP. Epub 2020 Nov 23.

The emerging 4D printing techniques open new horizons for fabricating self-actuated deformable objects by combing strength of 3D printing and stimuli-responsive shape memory materials. This work focuses on designing self-actuated deformable solids for 4D printing such that a solid can be programmed into a temporary shape and later recovers to its original shape after heating. To avoid a high material cost, we choose a dual-material strategy that mixes an expensive thermo-responsive shape memory polymer (SMP) material with a common elastic material, % for fabricating objects, which however leads to undesired deformation at the shape programming stage. We model this shape programming process as two elastic models with different parameters linked by a median shape based on customizing a constitutive model of thermo-responsive SMPs. Taking this material modeling as a foundation, we formulate our design problem as a nonconvex optimization to find the distribution of SMP materials over the whole object as well as the median shape, and develop an efficient and parallelizable method to solve it. We show that our proposed approach is able to design self-actuated deformable objects that cannot be achieved by state of the art approaches, and demonstrate their usefulness with three example applications.
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http://dx.doi.org/10.1109/TVCG.2020.3039613DOI Listing
November 2020
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