Publications by authors named "Song Liu"

1,030 Publications

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The Effect of High-Density Built Environments on Elderly Individuals' Physical Health: A Cross-Sectional Study in Guangzhou, China.

Int J Environ Res Public Health 2021 Sep 29;18(19). Epub 2021 Sep 29.

School of Geography and Planning, Sun Yat-sen University, Guangzhou 510275, China.

The built environment refers to the objective material environment built by humans in cities for living and production activities. Existing studies have proven that the built environment plays a significant role in human health, but little attention is paid to the elderly in this regard. At the same time, existing studies are mainly concentrated in Western developed countries, and there are few empirical studies in developing countries such as China. Based on POI (point of interest) data and 882 questionnaires collected from 20 neighborhoods in Guangzhou, we employ multilevel linear regression modeling, mediating effect modeling, to explore the path and mechanism of the impact of the built environment on elderly individuals' physical health, especially the mediating effects of physical and social interaction activity. The results show that the number of POIs, the distance to the nearest park and square, and the number of parks and squares are significantly positively correlated with the physical health of the elderly, while the number of bus and subway stations and the distance to the nearest station are significantly negatively correlated. Secondly, physical activity and social networks play a separate role in mediating the effect of the built environment on elderly individuals' physical health. The results enrich the research on the built environment and elderly individuals' health in the context of high-density cities in China and provide some reference basis for actively promoting spatial intervention and cultivating a healthy aging society.
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http://dx.doi.org/10.3390/ijerph181910250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508494PMC
September 2021

Van Der Waals gap-rich BiOCl atomic layers realizing efficient, pure-water CO-to-CO photocatalysis.

Nat Commun 2021 Oct 11;12(1):5923. Epub 2021 Oct 11.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Institute of Environmental & Applied Chemistry, College of Chemistry, Central China Normal University, 152 Luoyu Road, 430079, Wuhan, China.

Photocatalytic CO reduction (PCR) is able to convert solar energy into chemicals, fuels, and feedstocks, but limited by the deficiencies of photocatalysts in steering photon-to-electron conversion and activating CO, especially in pure water. Here we report an efficient, pure water CO-to-CO conversion photocatalyzed by sub-3-nm-thick BiOCl nanosheets with van der Waals gaps (VDWGs) on the two-dimensional facets, a graphene-analog motif distinct from the majority of previously reported nanosheets usually bearing VDWGs on the lateral facets. Compared with bulk BiOCl, the VDWGs-rich atomic layers possess a weaker excitonic confinement power to decrease exciton binding energy from 137 to 36 meV, consequently yielding a 50-fold enhancement in the bulk charge separation efficiency. Moreover, the VDWGs facilitate the formation of VDWG-Bi-V-Bi defect, a highly active site to accelerate the CO-to-CO transformation via the synchronous optimization of CO activation, *COOH splitting, and *CO desorption. The improvements in both exciton-to-electron and CO-to-CO conversions result in a visible light PCR rate of 188.2 μmol g h in pure water without any co-catalysts, hole scavengers, or organic solvents. These results suggest that increasing VDWG exposure is a way for designing high-performance solar-fuel generation systems.
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http://dx.doi.org/10.1038/s41467-021-26219-6DOI Listing
October 2021

Modulation of macrophages by a paeoniflorin-loaded hyaluronic acid-based hydrogel promotes diabetic wound healing.

Mater Today Bio 2021 Sep 21;12:100139. Epub 2021 Sep 21.

School of Pharmaceutical Sciences, Jilin University, Changchun, 130021, China.

The impaired wound healing in diabetes is a central concern of healthcare worldwide. However, current treatments often fail due to the complexity of diabetic wounds, and thus, emerging therapeutic approaches are needed. Macrophages, a prominent immune cell in the wound, play key roles in tissue repair and regeneration. Recent evidence has demonstrated that macrophages in diabetic wounds maintain a persistent proinflammatory phenotype that causes the failure of healing. Therefore, modulation of macrophages provides great promise for wound healing in diabetic patients. In this study, the potential of paeoniflorin (PF, a chemical compound derived from the herb ) for the transition of macrophages from M1 (proinflammatory phenotype) to M2 (anti-inflammatory/prohealing phenotype) was confirmed using and experimental approaches. A hydrogel based on high molecular weight hyaluronic acid (HA) was developed for local administration of PF in experimental diabetic mice with a full-thickness wound. The resultant formulation (HA-PF) was able to significantly promote cutaneous healing as compared to INTRASITE Gel (a commercial hydrogel wound dressing). This outcome was accompanied by the amelioration of inflammation, the improvement of angiogenesis, and re-epithelialization, and the deposition of collagen. Our study indicates the significant potential of HA-PF for clinical translation in diabetic wound healing.
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http://dx.doi.org/10.1016/j.mtbio.2021.100139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488309PMC
September 2021

Induction of cell death in ovarian cancer cells by doxorubicin and oncolytic vaccinia virus is associated with CREB3L1 activation.

Mol Ther Oncolytics 2021 Dec 29;23:38-50. Epub 2021 Apr 29.

Department of Immunology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA.

We have demonstrated that oncolytic vaccinia virus synergizes with doxorubicin (DOX) in inducing immunogenic cell death in platinum-resistant ovarian cancer cells and increases survival in syngeneic and xenograft tumor models. However, the mechanisms underlying the virus- and doxorubicin-mediated cancer cell death remain unknown. In this study, we investigated the effect of the oncolytic virus and doxorubicin used alone or in combination on activation of the cytoplasmic transcription factor CREB3L1 (cyclic AMP [cAMP] response element-binding protein 3-like 1) in ovarian cancer cell lines and clinical specimens. We demonstrated that doxorubicin-mediated cell death in ovarian cancer cell lines was associated with nuclear translocation of CREB3L1 and that the effect was augmented by infection with oncolytic vaccinia virus or treatment with recombinant interferon (IFN)-β used as a viral surrogate. This combination treatment was also effective in mediating nuclear translocation of CREB3L1 in cancer cells isolated from ovarian tumor biopsies at different stages of disease progression. The measurement of CREB3L1 expression in clinical specimens of ovarian cancer revealed lack of correlation with the stage of disease progression, suggesting that understanding the mechanisms of nuclear accumulation of CREB3L1 after doxorubicin treatment alone or in combination with oncolytic virotherapy may lead to the development of more effective treatment strategies against ovarian cancer.
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http://dx.doi.org/10.1016/j.omto.2021.04.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479291PMC
December 2021

Inhibition of CDK9 activity compromises global splicing in prostate cancer cells.

RNA Biol 2021 Sep 30:1-8. Epub 2021 Sep 30.

Department of Biochemistry and Developmental Biology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.

Cyclin-dependent kinase 9 (CDK9) phosphorylates RNA polymerase II to promote productive transcription elongation. Here we show that short-term CDK9 inhibition affects the splicing of thousands of mRNAs. CDK9 inhibition impairs global splicing and there is no evidence for a coordinated response between the alternative splicing and the overall transcriptome. Alternative splicing is a feature of aggressive prostate cancer (CRPC) and enables the generation of the anti-androgen resistant version of the ligand-independent androgen receptor, AR-v7. We show that CDK9 inhibition results in the loss of AR and AR-v7 expression due to the defects in splicing, which sensitizes CRPC cells to androgen deprivation. Finally, we demonstrate that CDK9 expression increases as PC cells develop CRPC-phenotype both and also in patient samples. To conclude, here we show that CDK9 inhibition compromises splicing in PC cells, which can be capitalized on by targeting the PC-specific addiction androgen receptor.
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http://dx.doi.org/10.1080/15476286.2021.1983287DOI Listing
September 2021

Role of Fucoxanthin towards Cadmium-induced renal impairment with the antioxidant and anti-lipid peroxide activities.

Bioengineered 2021 Dec;12(1):7235-7247

CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Kidney damages caused by cadmium are considered to be one of the most dangerous consequences for the human body. This study aimed to investigate the protective effects of fucoxanthin supplementation on mice models subjected to cadmium-induced kidney damage. The mice treated with cadmium chloride (CdCl) were observed to have significantly reduced the cross-section area of glomeruli. Cadmium exposure has also caused the damage of the structural integrity of mitochondria and increased blood urea nitrogen (BUN), kidney injury molecule 1 (KIM1), and neutrophil gelatinase associated lipocalin (NGAL) levels. Peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) levels in cadmium-exposed mice were markedly declined. Caspase3, caspase8, and caspase9 gene expressions in association with apoptosis were dramatically elevated in renal tissues. The CdCl treated mice were orally administered with 50 mg/kg Shenfukang, 10 mg/kg, 25 mg/kg, and 50 mg/kg fucoxanthin for 14 days. The results revealed that high doses of fucoxanthin administration significantly decreased BUN, KIM1, NGAL levels, increasing POD, SOD, CAT, and ascorbate APX levels. Fucoxanthin administration also promoted recovery of the renal functions, micro-structural organization, and ultra-structural organization in the renal cells. In summary, the ameliorative effects of fucoxanthin supplementation against cadmium-induced kidney damage were mediated via inhibiting oxidative stress and apoptosis, promoting the recovery of structural integrity of mitochondria.
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http://dx.doi.org/10.1080/21655979.2021.1973875DOI Listing
December 2021

Effective immobilization of heavy metals via reactive barrier by rhizosphere bacteria and their biofilms.

Environ Res 2021 Sep 24:112080. Epub 2021 Sep 24.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China. Electronic address:

As the portal of plants, rhizosphere microorganisms play an essential role in controlling the species, transformation, and bioavailability of heavy metals, yet the potential passivation mechanism is still unclear. In this study, two heavy metal resistant and growth-promoting rhizosphere bacteria were screened, and their mechanisms in dealing with external stress and immobilizing heavy metal were explored. The results showed that heavy metals inhibited the ability of Pseudomonas sp. H13 and Brevundomonas sp. H16 to promote plant growth, but stimulated the production of extracellular polysaccharides and inorganic labile sulfide, and enhanced biofilm formation, thereby significantly improved the removal efficiency of Cu, Zn, Cd, and Pb. Compared with H16, the biofilm of H13 disintegrated rapidly in the later stage, so more metal ions were adsorbed on the planktonic cells. The C-OH and PO groups related to polysaccharides play a crucial role in heavy metal adsorption, and the immobilization mechanism of the planktonic cell is mainly ion exchange and group complex, but for H16, intracellular enrichment cannot be ignored. Functional group complexes played a dominant role in biofilm, and the immobilized heavy metals were more difficult to release into the environment. This study highlighted the potential application prospects of biofilm bacteria in heavy metal remediation and explained the reactive barrier of rhizosphere bacteria to heavy metals.
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http://dx.doi.org/10.1016/j.envres.2021.112080DOI Listing
September 2021

Visualized Multigene Editing System for .

ACS Synth Biol 2021 Oct 24;10(10):2607-2616. Epub 2021 Sep 24.

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.

The resistance markers could ensure the entry of the CRISPR/Cas9 system into cells instead of gene editing. To increase the efficiency of positive colony screening on the primary transformation plates, we designed a visualized multigene editing system (VMS) via a unique tRNA-guide RNA (gRNA) array containing the gRNAs of a pigment gene and target genes. Disruption of produces white colonies, and the sequences of the endogenous tRNA, tRNA, tRNA, tRNA, and tRNA enhance gRNA release. The disruption efficiencies of multigene were analyzed in the strain AG11 using , , , , and as reporters. In white colonies on the primary transformation plates, the disruption rates of one-, two-, three-, four-, and five-target genes reached 89.2, 70.91, 50, 22.41, and 4.17%, respectively. The VMS developed here provides an effective method for screening homokaryotic multigene editing strains of .
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http://dx.doi.org/10.1021/acssynbio.1c00231DOI Listing
October 2021

Ultrahigh-Resolution, Label-Free Hyperlens Imaging in the Mid-IR.

Nano Lett 2021 10 17;21(19):7921-7928. Epub 2021 Sep 17.

Department of Mechanical Engineering, Vanderbilt University, Nashville, Tennessee 37212, United States.

The hyperbolic phonon polaritons supported in hexagonal boron nitride (hBN) with long scattering lifetimes are advantageous for applications such as super-resolution imaging via hyperlensing. Yet, hyperlens imaging is challenging for distinguishing individual and closely spaced objects and for correlating the complicated hyperlens fields with the structure of an unknown object underneath. Here, we make significant strides to overcome each of these challenges. First, we demonstrate that monoisotopic hBN provides significant improvements in spatial resolution, experimentally resolving structures as small as 44 nm and those with sub 25 nm spacings at 6.76 μm free-space wavelength. We also present an image reconstruction algorithm that provides a structurally accurate, visual representation of the embedded objects from the complex hyperlens field. Further, we offer additional insights into optimizing hyperlens performance on the basis of material properties, with an eye toward realizing far-field imaging modalities. Thus, our results significantly advance label-free, high-resolution, spectrally selective hyperlens imaging and image reconstruction methodologies.
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http://dx.doi.org/10.1021/acs.nanolett.1c01808DOI Listing
October 2021

Prediction of serosal invasion in gastric cancer: development and validation of multivariate models integrating preoperative clinicopathological features and radiographic findings based on late arterial phase CT images.

BMC Cancer 2021 Sep 16;21(1):1038. Epub 2021 Sep 16.

Department of Radiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, No.321, Zhongshan Road, Nanjing City, 210008, Jiangsu Province, China.

Background: To develop and validate multivariate models integrating endoscopic biopsy, tumor markers, and CT findings based on late arterial phase (LAP) to predict serosal invasion in gastric cancer (GC).

Methods: The preoperative differentiation degree, tumor markers, CT morphological characteristics, and CT value-related and texture parameters of 154 patients with GC were analyzed retrospectively. Multivariate models based on regression analysis and machine learning algorithms were performed to improve the diagnostic efficacy.

Results: The differentiation degree, carbohydrate antigen (CA) 199, CA724, CA242, and multiple CT findings based on LAP differed significantly between T1-3 and T4 GCs in the primary cohort (all P < 0.05). Multivariate models based on regression analysis and random forest achieved AUCs of 0.849 and 0.865 in the primary cohort, respectively.

Conclusion: We developed and validated multivariate models integrating endoscopic biopsy, tumor markers, CT morphological characteristics, and CT value-related and texture parameters to predict serosal invasion in GCs and achieved favorable performance.
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http://dx.doi.org/10.1186/s12885-021-08672-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447770PMC
September 2021

The short-term and long-term outcomes of indocyanine green tracer-guided laparoscopic radical gastrectomy in patients with gastric cancer.

World J Surg Oncol 2021 Sep 9;19(1):271. Epub 2021 Sep 9.

Department of General Surgery, Drum Tower Medical College of Nanjing Medical University, 321 Zhongshan Road, Nanjing, 210008, Jiangsu, China.

Background: The safety and efficacy of indocyanine green (ICG) imaging navigational laparoscopic gastrectomy remain controversial. This study is to evaluate the short-term and long-term outcomes of ICG-guided laparoscopic radial gastrectomy in patients with gastric cancer.

Methods: Consecutive patients with definitive diagnosis of gastric cancer that underwent laparoscopic radical gastrectomy were collected retrospectively. Propensity score matching (PSM) at 1:1 ratio was performed to compare the outcomes of two groups.

Results: A total of 122 qualified patients were divided into ICG group (n = 34) and non-ICG group (n = 88). PSM yielded 28 patients with comparable baseline characteristics into each group. The number of retrieved lymph node in ICG group was significantly higher than that in non-ICG group (P = 0.0196). There was no statistical difference of perioperative, short-term, and long-term complications between the two groups.

Conclusion: ICG-guided laparoscopic radical gastrectomy is safe and effective, and ICG-navigated lymphadenectomy improves the number of retrieved lymph nodes for patients with gastric cancer.
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http://dx.doi.org/10.1186/s12957-021-02385-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431906PMC
September 2021

Biodegradation of sulfonamide antibiotics through the heterologous expression of laccases from bacteria and investigation of their potential degradation pathways.

J Hazard Mater 2021 08 13;416:125815. Epub 2021 Apr 13.

Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, PR China; SynBio Research Platform, Collaborative Innovation Centre of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, PR China. Electronic address:

In this study, seven laccase genes from different bacteria were linked with the signal peptides PelB, Lpp or Ompa for heterologous expression in E. coli. The recombinant strains were applied for the removal of sulfadiazine (SDZ), sulfamethazine (SMZ), and sulfamethoxazole (SMX). The results obtained for different signal peptides did not provide insights into the removal mechanism. The removal ratios of SDZ, SMZ, and SMX obtained with the recombinant strain 6#P at 60 h were around 92.0%, 89.0%, and 88.0%, respectively. The degradation pathways of sulfonamides have been proposed, including SO elimination, hydroxylation, oxidation, pyrimidine ring cleavage, and N-S bond cleavage. Different mediators participate in the degradation of antibiotics through different mechanisms, and different antibiotics have different responses to the same mediator. The addition of 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) slightly promoted the removal of sulfonamides by most recombinant strains with different signal peptides, especially for the recombinant strain 2#O. The removal of sulfonamides by 1-hydroxybenzotriazole (HBT) varied with the recombinant strains. Syringaldehyde (SA) had a slight inhibitory effect on the removal of sulfonamides, with the most significant effect on strains 7#L and 7#O.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125815DOI Listing
August 2021

Nitrogen dioxide decline and rebound observed by GOME-2 and TROPOMI during COVID-19 pandemic.

Air Qual Atmos Health 2021 Aug 28:1-19. Epub 2021 Aug 28.

Deutsches Zentrum für Luft- und Raumfahrt (DLR), Institut für Methodik der Fernerkundung (IMF), Oberpfaffenhofen, Germany.

Since its first confirmed case in December 2019, coronavirus disease 2019 (COVID-19) has become a worldwide pandemic with more than 90 million confirmed cases by January 2021. Countries around the world have enforced lockdown measures to prevent the spread of the virus, introducing a temporal change of air pollutants such as nitrogen dioxide (NO) that are strongly related to transportation, industry, and energy. In this study, NO variations over regions with strong responses to COVID-19 are analysed using datasets from the Global Ozone Monitoring Experiment-2 (GOME-2) sensor aboard the EUMETSAT Metop satellites and TROPOspheric Monitoring Instrument (TROPOMI) aboard the EU/ESA Sentinel-5 Precursor satellite. The global GOME-2 and TROPOMI NO datasets are generated at the German Aerospace Center (DLR) using harmonized retrieval algorithms; potential influences of the long-term trend and seasonal cycle, as well as the short-term meteorological variation, are taken into account statistically. We present the application of the GOME-2 data to analyze the lockdown-related NO variations for morning conditions. Consistent NO variations are observed for the GOME-2 measurements and the early afternoon TROPOMI data: regions with strong social responses to COVID-19 in Asia, Europe, North America, and South America show strong NO reductions of 30-50% on average due to restriction of social and economic activities, followed by a gradual rebound with lifted restriction measures.

Supplementary Information: The online version contains supplementary material available at 10.1007/s11869-021-01046-2.
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http://dx.doi.org/10.1007/s11869-021-01046-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397874PMC
August 2021

Growth mechanism and atomic structure of group-IIA compound-promoted CVD-synthesized monolayer transition metal dichalcogenides.

Nanoscale 2021 Aug 22;13(30):13030-13041. Epub 2021 Jul 22.

Science and Technology on Advanced Ceramic Fibers and Composites Laboratory, College of Aerospace Science and Engineering, National University of Defense Technology, Changsha 410073, P. R. China.

Developing promoters that can boost the growth quality, efficiency, and robustness of two-dimensional (2D) transition metal dichalcogenides is significant for their industrial applications. Herein a new group (group IIA) of promoters in the periodic table has been disclosed, whose chlorides (especially CaCl and SrCl) exhibit a versatile promoting effect on the CVD growth of various TMD monolayers, including hexagonal MoS, MoSe, Re doped MoS, and triclinic ReS. The promoting effect of group IIA promoters relies on the appropriate dose and is strongly substrate-dependent. The performances of five typical group IA-IIA metal chlorides are ranked by quantitative investigations, displaying periodic variations closely related to the electronegativities of the metal elements. A brand-new acid-base match model is proposed, attributing the promoting mechanism to an increase of the substrate basicity due to the usage of promoters, thus leading to the sufficient adsorption of the acidic precursor. Aberration-corrected annular dark field scanning transmission electron microscopy (ADF-STEM) was applied, unveiling anomalous grain boundaries (GBs) with a low density of coincident sites in the as-grown ReS and detailed atomic configurations of Re doped MoS. This work expands the promoter library and gives an insight into GB engineering for the CVD growth of 2D TMDs.
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http://dx.doi.org/10.1039/d1nr03273aDOI Listing
August 2021

Central functional reorganization and recovery following facial-hypoglossal neurorrhaphy for facial paralysis.

Neuroimage Clin 2021 Aug 23;32:102782. Epub 2021 Aug 23.

Department of Injury and Repair, and Beijing Key Laboratory of Central Nervous System Injury, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100070, China; Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; U 1195, INSERM and Université Paris-Saclay, 94276 Le Kremlin-Bicêtre, France. Electronic address:

Objective: Functional deficits induced by nerve injuries can be restored by achieving effective reinnervation of the denervated targets and functional reorganization of the central nervous system after nerve reconstruction. In this study, we investigated the effect and extent of cortical functional reorganization related to the ability of transferred hypoglossal neurons to restore facial function in facial paralysis patients after a surgical bridge of neurorrhaphy ectopically between the ipsilateral hypoglossal nerve and injured facial nerve.

Methods: We treated 23 patients (35.4 ± 10.3 years, 10 males) and followed them up for 2.9 ± 0.61 years. We used motor-task-related functional magnetic resonance imaging to map activation change at multiple time points before and after neurorrhaphy; 20 normal subjects were included as control.

Results: All patients regained facial function to some extent after neurorrhaphy. Enhanced activation in motor-related cortices gradually returned to normal levels and was positively correlated with regained facial function. The related cortical functional areas included the left middle temporal gyrus, left inferior frontal gyrus, insula, bilateral motor cortex and the supplementary motor area extending to the paracingulate involved in intensive eye closing, as well as the left superior temporal gyrus, right putamen and the bilateral motor cortex involved in lip pursing. Intriguingly, significant correlations were found between the pre-surgery activation while intensive eye closing in bilateral motor cortex and recovery of facial nerve function induced by the neurorrhaphy treatment.

Conclusion: This is the first study mapping activation change in motor cortices at multiple time points before and after repair of the facial nerve. The cortex functional reorganization found may suggest potential treatment targets in the central nervous system for adjuvant therapies such as repetitive transcranial magnetic stimulation to further improve functional recovery.
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http://dx.doi.org/10.1016/j.nicl.2021.102782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405913PMC
August 2021

Apolipoprotein C-II induces EMT to promote gastric cancer peritoneal metastasis via PI3K/AKT/mTOR pathway.

Clin Transl Med 2021 Aug;11(8):e522

Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Background: Peritoneal metastasis (PM) occurs frequently in patients with gastric cancer (GC) and confers poor survival. Lipid metabolism acts as a non-negligible regulator in epithelial-mesenchymal transition (EMT), which is crucial for the metastasis of GC. As apolipoprotein C2 (APOC2) is a key activator of lipoprotein lipase for triglyceride metabolism, the exact mechanism of APOC2 remains largely unknown in GC.

Methods: Tandem mass tags identified differentially expressed proteins between human PM and GC tissues, and showed that APOC2 overexpressed in PM tissues, which was further confirmed by immunoblotting, immunohistochemistry, and ELISA. Global gene expression changes were identified in APOC2 knockdown cells via RNA-sequencing. The role of APOC2 in lipid metabolism of GC cells was assessed via the Seahorse XF analyzer and lipid staining assays. The biological role of APOC2 in GC cells was determined by 3D Spheroid invasion, apoptosis, colony formation, wound healing, transwell assay, and mouse models. The interaction between APOC2 and CD36 was analyzed by co-immunoprecipitation and biolayer interferometry. The underlying mechanisms were investigated using western blot technique.

Results: APOC2 overexpressed in GC PM tissues. Upregulation of APOC2 correlated with a poor prognosis in GC patients. APOC2 promoted GC cell invasion, migration, and proliferation via CD36-mediated PI3K/AKT/mTOR signaling activation. Furthermore, APOC2-CD36 axis upregulated EMT markers of GC cells via increasing the phosphorylation of PI3K, AKT, and mTOR. Knockdown either APOC2 or CD36 inhibited the malignant phenotype of cancer cells, and delayed GC PM progression in murine GC models.

Conclusion: APOC2 cooperates with CD36 to induce EMT to promote GC PM via PI3K/AKT/mTOR pathway. APOC2-CD36 axis may be a potential target for the treatment of aggressive GC.
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http://dx.doi.org/10.1002/ctm2.522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351524PMC
August 2021

Metabonomics analysis of drought resistance of wheat seedlings induced by β-aminobutyric acid-modified chitooligosaccharide derivative.

Carbohydr Polym 2021 Nov 15;272:118437. Epub 2021 Jul 15.

CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), No. 1 Wenhai Road, Qingdao 266237, China.

Chitooligosaccharide grafted with β-aminobutyric acid based on the idea of bioactive molecular splicing was prepared, and the differences in drought resistance activity before and after grafting were compared. The mechanism was investigated by comparing the differences of the derivative with the Control and Drought about metabolomes. The results showed that the expected derivative was successfully synthesized, named COS-BABA, and had better drought resistance-inducing activity than the raw materials. We suggest that COS-BABA induced drought resistance through second messenger-induced activation of signaling pathways related to traumatic acid and indol-3-lactic acid, which enhanced nucleic acid metabolism to accumulate nucleotides and decreased some amino acids to facilitate protein synthesis. These proteins are regulated to strengthen photosynthesis, resulting in the promotion of carbohydrate metabolism. The accumulation of unsaturated fatty acids stabilized the cell membrane structure and prevented nonstomatal water dissipation. This study provides ideas for the development of more effective drought resistance inducers.
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http://dx.doi.org/10.1016/j.carbpol.2021.118437DOI Listing
November 2021

Clinical application of gelatin sponge microparticles combined with pirarubicin for hepatic transcatheter arterial chemoembolization in breast cancer liver metastasis treatment: results of a single-center long-term study.

World J Surg Oncol 2021 Aug 21;19(1):249. Epub 2021 Aug 21.

Hepatobiliary and Pancreatic Center, Beijing Tsinghua Changgung Hospital, 168 Litang Road, Beijing, 102218, Changping District, China.

Objective: To retrospectively analyze the safety and long-term clinical efficacy of gelatin sponge microparticles combined with the chemotherapy drug pirarubicin for hepatic transcatheter arterial chemoembolization (GSMs-TACE) in order to treat breast cancer liver metastasis (BCLM).

Methods: Twenty-seven BCLM patients who underwent GSMs-TACE from July 2010 to July 2016 were enrolled. Tumor target blood vessels were slowly and regionally embolized with absorbable gelatin sponge particles and pirarubicin injections. Plain computed tomography (CT) scans and biochemical indexes were re-examined at 4 days after treatment, and enhanced CT scans or magnetic resonance images and biochemical indexes, 1 month later. For patients with stable tumors, the follow-up period was 2 to 3 months, and the tumor response was evaluated using Modified Response Evaluation Criteria in Solid Tumors. Adverse reactions, survival time, and prognostic factors were assessed.

Results: By October 2019, 27 patients with BCLM had undergone GSMs-TACE, with an average of 2.44 ± 1.58 treatments. The 1-, 3-, and 5-year survival rates were 62.96%, 22.22%, and 14.81%, respectively, and the mOS was 22.0 months. No serious complications, such as acute liver failure and liver abscess, had occurred. There were two cases of acute cholecystitis that recovered after symptomatic treatment. Multivariate analysis of the prognosis showed that the primary tumor size, number of metastatic lymph nodes, estrogen receptor/progesterone receptor (ER/PR) status, and time to postoperative liver metastasis and combination therapy were statistically significant.

Conclusions: The overall prognosis of BCLM was poor. GSMs-TACE was safe and effective for BCLM treatment and could prolong the median survival time of patients. Therefore, it is worthy of widespread clinical application.
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http://dx.doi.org/10.1186/s12957-021-02332-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8380398PMC
August 2021

Dynamic Observation of Autophagy and Transcriptome Profiles in a Mouse Model of Bleomycin-Induced Pulmonary Fibrosis.

Front Mol Biosci 2021 29;8:664913. Epub 2021 Jul 29.

Department of Pulmonary and Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Pulmonary fibrosis is a group of progressive, fibrotic, and fatal lung diseases, and the role of autophagy in pulmonary fibrosis is controversial. In the current research, we dynamically observed a bleomycin-induced pulmonary fibrosis mouse model after 3, 7, 14, 21, and 28 days and investigated the expression of autophagy markers. We found that autophagy markers were not significantly changed on the indicated days in the mouse lung tissue. Then, RNA-Seq was used to analyze the gene expression and associated functions and pathways in fibrotic lung tissue on different days post-bleomycin. In addition, short time series expression miner (STEM) analysis was performed to explore the temporal post-bleomycin gene expression. Through STEM, continually up- or downregulated profiles did not demonstrate the critical role of autophagy in the development of fibrosis. Furthermore, gene ontology (GO) annotations showed that continually upregulated profiles were mainly related to fibrosis synthesis, extracellular space, and inflammation, while enriched pathways were mainly related to the PI3K-Akt signaling pathway, ECM-receptor interactions, and focal adhesion signaling pathway. For continually downregulated profiles, GO annotations mainly involved sarcomere organization, muscle contraction, and muscle fiber development. The enriched KEGG signaling pathways were the cAMP signaling pathway, cGMP-PKG signaling pathway, calcium signaling pathway, and cardiac muscle contraction. Moreover, we analyzed autophagy-related genes' expression in specific cells from a publicly available database of three human and one animal study of pulmonary fibrosis using single-cell sequencing technology. All results consistently demonstrated no critical role of autophagy in the pathogenesis of pulmonary fibrosis. In summary, autophagy may not critically and consistently change during the development of pulmonary fibrosis at different stages post-bleomycin in a mouse model. These continually up- or downregulated profiles, including gene profiles, and the corresponding functions and pathways may provide mechanistic insights into IPF therapy.
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http://dx.doi.org/10.3389/fmolb.2021.664913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358296PMC
July 2021

Mechanisms Underlying the Effects of Landscape Features of Urban Community Parks on Health-Related Feelings of Users.

Int J Environ Res Public Health 2021 07 26;18(15). Epub 2021 Jul 26.

College of Architecture and Urban Planning, Tongji University, Shanghai 200092, China.

Urban community parks are closely related to the health of residents, and have a positive effect on residents' perception of nature, alleviating anxiety, and promoting physical health. Many previous studies have examined the impact of community parks on the health of the population, but few studies have investigated the potential of specific landscape elements in community parks to restore physical health. We conducted psychological questionnaires with 440 users of community parks in Shanghai through on-site surveys. Based on the psychological questionnaire, a structural equation model of the relationship between the community park landscape environment and users' feelings was established. The model indicated that the natural environment, activity environment, and rest environment in the community park had positive effects on the physical, mental, and social health of users. At the same time, we recruited 50 participants to conduct laboratory experiments examining physiological changes while participants viewed different types of scene photographs showing the same landscape element. By measuring physiological indicators, including skin conductivity and heart rate, we sought to identify the types of landscape elements that help relieve the stress of users. The results revealed that flower clusters and waterscapes in the natural environment landscape, plastic fitness trails and fitness equipment places in the sports area, landscape elements such as benches with backrests, Chinese style pavilions, and green corridors with plants in the rest space, played positive roles in alleviating feelings of pressure and promoting relaxation among community park users. Based on these findings, we propose specific design strategies to improve the landscape health of community parks.
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http://dx.doi.org/10.3390/ijerph18157888DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345660PMC
July 2021

Immune cytolytic activity is associated with reduced intra-tumoral genetic heterogeneity and with better clinical outcomes in triple negative breast cancer.

Am J Cancer Res 2021 15;11(7):3628-3644. Epub 2021 Jul 15.

Department of Surgical Oncology, Roswell Park Cancer Institute Buffalo, NY 14263, USA.

Evaluation of the functional aspects if the tumor immune microenvironment (TIME), such as the recently introduced cytolytic activity score (CYT) index have been under the spotlight in cancer research; however, clinical relevance of immune cell killing activity in breast cancer has never been analyzed in large patient cohorts. We hypothesized that CYT reflects the immune activity of TIME and can predict patient survival. A total of 7533 breast cancer patients were analyzed as both discovery and validation cohorts. We found that high CYT was associated with advanced histological grade and triple-negative breast cancer (TNBC). High CYT in tumors was significantly associated with better survival in TNBC, but unexpectedly, not in other breast cancer subtypes. High CYT TNBC included both favorable immune-related, as well as unfavorable (suppressive) inflammation-related gene sets, and characterized by high infiltration with T cells and B cells. High CYT TNBC was associated with high homologous recombination deficiency and low somatic copy number alteration score and less mutant allele tumor heterogeneity, but not with tumor mutation burden (TMB). Although CYT was not associated with pathological complete response after neoadjuvant chemotherapy, it was significantly associated with high expression of multiple immune checkpoint molecules. In conclusion, CYT of TNBC is associated with enhanced anti-cancer immunity, less intra-tumoral heterogeneity, and with better survival.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8332854PMC
July 2021

Strain Gri From the Tea Geometrid Moth Contributes to Its Host's Fecundity.

Front Microbiol 2021 19;12:694466. Epub 2021 Jul 19.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China.

Members of the genus manipulate insect-host reproduction and are the most abundant bacterial endosymbionts of insects. The tea Geometrid moth (Warren) (Lepidoptera: Geometridae) is the most devastating insect pest of tea plants [ (L.) O. Kuntze] in China. However, limited data on the diversity, typing, or phenotypes of in are available. Here, we used a culture-independent method to compare the gut bacteria of and other tea Geometridae moths. The results showed that the composition of core gut bacteria in larvae of the three Geometridae moth species was similar, except for the presence of . Moreover, was also present in adult female samples. A strain was isolated from and designated as Gri. Comparative analyses showed that this strain shared multilocus sequence types and surface protein hypervariable region profiles with cytoplasmic incompatibility (CI)-inducing strains in supergroup B; however, the Gri-associated phenotypes were undetermined. A reciprocal cross analysis showed that -uninfected females mated with infected males resulted in 100% embryo mortality (0% eggs hatched per female). Eggs produced by mating between uninfected males and infected females hatched normally. These findings indicated that Gri induces strong unidirectional CI in . Additionally, compared with uninfected females, -infected females produced approximately 30-40% more eggs. Together, these results show that this strain induces reproductive CI in and enhances the fecundity of its female host. We also demonstrated that Gri potential influences reproductive communication between and through CI.
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http://dx.doi.org/10.3389/fmicb.2021.694466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326765PMC
July 2021

Body fatness and breast cancer risk in relation to phosphorylated mTOR expression in a sample of predominately Black women.

Breast Cancer Res 2021 07 30;23(1):77. Epub 2021 Jul 30.

Department of Cancer Prevention and Control, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.

Background: The mechanistic target of rapamycin (mTOR) pathway promoted by positive energy imbalance and insulin-like growth factors can be a mechanism by which obesity influences breast cancer risk. We evaluated the associations of body fatness with the risk of breast cancer varied with phosphorylated (p)-mTOR protein expression, an indication of the pathway activation.

Methods: Women with newly diagnosed breast cancer (n = 715; 574 [80%] Black and 141 [20%] White) and non-cancer controls (n = 1983; 1280 [64%] Black and 713 [36%] White) were selected from the Women's Circle of Health Study. Surgical tumor samples among the cases were immunostained for p-mTOR (Ser2448) and classified as p-mTOR-overexpressed, if the expression level ≥ 75th percentile, or p-mTOR-negative/low otherwise. Anthropometrics were measured by trained staff, and body composition was determined by bioelectrical impedance analysis. Odds ratios (ORs) of p-mTOR-overexpressed tumors and p-mTOR-negative/low tumors compared to controls were estimated using polytomous logistic regression. The differences in the associations by the p-mTOR expression status were assessed by tests for heterogeneity.

Results: Cases with p-mTOR-overexpressed tumors, but not cases with p-mTOR-negative/low tumors, compared to controls were more likely to have higher body mass index (BMI), percent body fat, and fat mass index (P-heterogeneity < 0.05), although the OR estimates were not significant. For the measurement of central adiposity, cases with p-mTOR overexpressed tumors had a higher odds of being at the Q3 (OR = 2.52, 95% CI = 1.46 to 4.34) and Q4 (OR = 1.99, 95% CI = 1.12 to 3.50) of waist circumference (WC) compared to controls. Similarly, cases with p-mTOR overexpressed tumors had a higher odds of being at the Q3 (OR = 1.82, 95% CI = 1.11 to 2.98) and Q4 (OR = 1.81, 95% CI = 1.11 to 2.98) of WHR compared to controls. These associations of WC and waist-to-hip ratio (WHR) did not differ by tumor p-mTOR status (P-heterogeneity = 0.27 and 0.48, respectively).

Conclusions: Our findings suggest that in this population composed of predominately Black women, body fatness is associated with breast cancer differently for p-mTOR overexpression and p-mTOR negative/low expression. Whether mTOR plays a role in the obesity and breast cancer association warrants confirmation by prospective studies.
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http://dx.doi.org/10.1186/s13058-021-01458-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325192PMC
July 2021

Selective Adsorption and Separation of Proteins by Ligand-Modified Nanofiber Fabric.

Polymers (Basel) 2021 Jul 14;13(14). Epub 2021 Jul 14.

Department of Chemical Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 4648603, Japan.

Electrospun polyvinyl alcohol (PVA) nanofiber fabric was modified by Cibacron Blue F3GA (CB) to enhance the affinity of the fabric. Batch experiments were performed to study the nanofiber fabric's bovine hemoglobin (BHb) adsorption capacity at different protein concentrations before and after modification. The maximum BHb adsorption capacity of the modified nanofiber fabric was 686 mg/g, which was much larger than the 58 mg/g of the original fabric. After that, the effect of feed concentration and permeation rate on the dynamic adsorption behaviors for BHb of the nanofiber fabric was investigated. The pH impact on BHb and bovine serum albumin (BSA) adsorption was examined by static adsorption experiments of single protein solutions. The selective separation experiments of the BHb-BSA binary solution were carried out at the optimal pH value, and a high selectivity factor of 5.45 for BHb was achieved. Finally, the reusability of the nanofiber fabric was examined using three adsorption-elution cycle tests. This research demonstrated the potential of the CB-modified PVA nanofiber fabric in protein adsorption and selective separation.
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http://dx.doi.org/10.3390/polym13142313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309411PMC
July 2021

A folate-targeted PEGylated cyclodextrin-based nanoformulation achieves co-delivery of docetaxel and siRNA for colorectal cancer.

Int J Pharm 2021 Sep 14;606:120888. Epub 2021 Jul 14.

School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China; Pharmacodelivery Group, School of Pharmacy, University College Cork, Cork T12 YN60, Ireland. Electronic address:

Docetaxel (DTX) is a chemotherapeutic agent used for a range of cancers, but it has little activity against colorectal cancer (CRC). However, combination therapy with other therapeutic agents is a potential strategy to enhance the efficacy of DTX in CRC treatment. The nuclear factor-κB (NF-κB) signaling pathway is implicated in a variety of malignancies (e.g., CRC), and the blockade of NF-κB may increase the sensitivity of cancer cells to chemotherapy. The application of small interference RNA (siRNA) to inhibit the translation of complementary mRNA has demonstrated the potential for cancer gene therapy. In this study, an amphiphilic cationic cyclodextrin (CD) nanoparticle modified with PEGylated folate (FA; a ligand to target folate receptor on CRC) has been developed for co-delivery of DTX and siRNA (against the RelA, a subunit of NF-κB) in the treatment of CRC. The resultant co-formulation (CD.DTX.siRelA.PEG-FA) achieved cell-specific uptake indicating the function of the folate targeting ligand. The CD.DTX.siRelA.PEG-FA nanoparticle enhanced the apoptotic effect of DTX with the downregulation of RelA expression, which significantly retarded the growth of CRC in mice, without causing significant toxicity. These results suggest that the FA-targeted PEGylated CD-based co-formulation provides a promising strategy for combining DTX and siRNA in treating CRC.
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http://dx.doi.org/10.1016/j.ijpharm.2021.120888DOI Listing
September 2021

Reconfigurable Spin-Wave Interferometer at the Nanoscale.

Nano Lett 2021 Jul 16;21(14):6237-6244. Epub 2021 Jul 16.

Fert Beijing Institute, MIIT Key Laboratory of Spintronics, School of Integrated Circuit Science and Engineering, Beihang University, Beijing 100191, China.

Spin waves can transfer information free of electron transport and are promising for wave-based computing technologies with low-power consumption as a solution to severe energy losses in modern electronics. Logic circuits based on the spin-wave interference have been proposed for more than a decade, while it has yet been realized at the nanoscale. Here, we demonstrate the interference of spin waves with wavelengths down to 50 nm in a low-damping magnetic insulator. The constructive and destructive interference of spin waves is detected in the frequency domain using propagating spin-wave spectroscopy, which is further confirmed by the Brillouin light scattering. The interference pattern is found to be highly sensitive to the distance between two magnetic nanowires acting as spin-wave emitters. By controlling the magnetic configurations, one can switch the spin-wave interferometer on and off. Our demonstrations are thus key to the realization of spin-wave computing system based on nonvolatile nanomagnets.
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http://dx.doi.org/10.1021/acs.nanolett.1c02010DOI Listing
July 2021

Linking rare and abundant phoD-harboring bacteria with ecosystem multifunctionality in subtropical forests: From community diversity to environmental adaptation.

Sci Total Environ 2021 Nov 9;796:148943. Epub 2021 Jul 9.

Key Laboratory of Aquatic Botany and Watershed Ecology Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, PR China; Center of the Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan 430074, PR China. Electronic address:

Environmental factor-driven bacterial diversity could be an indicator for evaluating ecosystem multifunctionality (EMF). However, little is known about interconnections between EMF and the community diversity of rare and abundant phoD-harboring bacteria responsible for organic phosphorus mineralization. Illumina MiSeq sequencing and multiple statistical analyses were used to evaluate diversity maintenance of rare and abundant phoD-harboring bacteria at both taxonomic and phylogenetic levels and their contributions to soil EMF in the subtropical Shennongjia primeval forest. We found that rare phoD-harboring bacteria exhibited higher community diversity and broader environmental breadths than abundant ones, while abundant phoD-harboring bacteria showed closer phylogenetic clustering and stronger phylogenetic signals of ecological preferences than rare ones. Stochastic processes dominated community assemblies of rare and abundant phoD-harboring bacteria, and temperature was an important environmental variable adjusting the balance between stochastic and deterministic processes. The taxonomic α-diversity of rare phoD-harboring bacteria showed larger contribution to soil EMF than that of abundant ones, while the phylogenetic α-diversity of abundant phoD-harboring bacteria contributed significantly more than that of rare ones. Our findings enrich knowledge of the environmental adaptation of rare and abundant phoD-harboring bacteria, and highlight linkages between soil EMF and the diversity of rare and abundant phoD-harboring bacteria at both the taxonomic and phylogenetic levels.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148943DOI Listing
November 2021

Irinotecan eluting beads-transarterial chemoembolization using Callispheres® microspheres is an effective and safe approach in treating unresectable colorectal cancer liver metastases.

Ir J Med Sci 2021 Jul 15. Epub 2021 Jul 15.

Cancer Interventional Center, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001, Liaoning, China.

Background: Callispheres® microspheres (CSM) are the first drug-eluting bead (DEB) product developed in China; meanwhile, DEB-transarterial chemoembolization (TACE) with CSM is effective and safe in the treatment of hepatocellular carcinoma and intrahepatic cholangiocarcinoma. However, the data regarding the role of irinotecan-eluting beads-TACE (DEBIRI-TACE) using CSM for colorectal cancer liver metastases (CRLM) treatment is limited. Therefore, the present study aimed to investigate the efficacy and safety of DEBIRI-TACE using CSM in the patients with unresectable CRLM.

Methods: Totally, 42 unresectable CRLM patients treated with DEBIRI-TACE using CSM were continuously enrolled in this study. Postoperative treatment response (including complete response rate (CR), objective response rate (ORR), and disease control rate (DCR)), survival data (overall survival (OS)), liver function, and adverse events were documented during the follow-up.

Results: CR, ORR, and DCR were 19.0%, 92.9%, and 100.0%, respectively, at month (M) 1; were 23.8%, 92.9%, and 97.6%, respectively, at M3; then were 14.3%, 78.6%, and 90.5%, respectively at M6. Regarding survival profiles, 1-year OS was 81.0%; 2-year OS was 58.5%; median OS was 25.0 months (95%CI: 19.3-30.7 months). Additionally, ALT and AST experienced an obviously increased trend at 4 days, but a declined trend at 7 days, while ALB and TBIL had no obvious change. No grade 3 or grade 4 adverse event was observed, and main adverse events included fever (95.3%), pain (57.1%), fatigue (50.0%), and nausea/vomiting (42.8%).

Conclusion: DEBIRI-TACE with CSM achieves high treatment response, acceptable survival benefits, and good toleration in unresectable CRLM treatment.
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http://dx.doi.org/10.1007/s11845-021-02629-9DOI Listing
July 2021

Comparative short-term and long-term outcomes between internal and external intestinal plication in the management of small bowel obstruction.

BMC Surg 2021 Jul 12;21(1):309. Epub 2021 Jul 12.

Department of Gastrointestinal Surgery, Nanjing Drum Tower Hospital, 321 Zhongshan RD, Nanjing, China.

Background: Small bowel obstruction (SBO) is common and usually requires surgical intervention. Intestinal plication is a traditional but critical strategy for SBO in certain scenarios. This study is to compare the short-term and long-term outcome between internal and external plications in the management of SBO.

Methods: All patients receiving intestinal plication in our hospital were retrospectively collected. Short-term outcome including postoperative complications, reoperation, postoperative ICU stay, starting day of liquid diet and postoperative hospitalization, as well as long-term outcome including recurrence of obstruction, readmission, reoperation and death were compared between groups. Gut function at annual follow-up visits was evaluated as well.

Results: Nine internal and 11 external candidates were recruited into each group. The major causes of plication were adhesive obstruction, abdominal cocoon, volvulus and intussusception. Lower incidence of postoperative complication (p = 0.043) and shorter postoperative hospitalization (p = 0.049) was observed in internal group. One patient receiving external plication died from anastomosis leakage. During the 5-year follow-up period, the readmission rate was low in both groups (22.2 % vs. 9.1 %), and none of patients required reoperation or deceased. None of patients exhibited gut dysfunction, and all patients restored normal gut function after 4 years. Patients in external group demonstrated accelerated recovery of gut function after surgery.

Conclusions: This study compares short-term and long-term outcome of patients receiving internal or external intestinal plication. We suggest a conservative attitude toward external plication strategy. Surgical indication for intestinal plication is critical and awaits future investigations.
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http://dx.doi.org/10.1186/s12893-021-01304-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276395PMC
July 2021
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