Publications by authors named "Song Ke"

138 Publications

Acetabular Edge Loading During Gait Is Elevated by the Anatomical Deformities of Hip Dysplasia.

Front Sports Act Living 2021 1;3:687419. Epub 2021 Jul 1.

Program in Physical Therapy, Movement Science Research Center, Washington University in St. Louis School of Medicine, St. Louis, MO, United States.

Developmental dysplasia of the hip (DDH) is a known risk factor for articular tissue damage and secondary hip osteoarthritis. Acetabular labral tears are prevalent in hips with DDH and may result from excessive loading at the edge of the shallow acetabulum. Location-specific risks for labral tears may also depend on neuromuscular factors such as movement patterns and muscle-induced hip joint reaction forces (JRFs). To evaluate such mechanically-induced risks, we used subject-specific musculoskeletal models to compare acetabular edge loading (AEL) during gait between individuals with DDH ( = 15) and healthy controls ( = 15), and determined the associations between AEL and radiographic measures of DDH acetabular anatomy. The three-dimensional pelvis and femur anatomy of each DDH and control subject were reconstructed from magnetic resonance images and used to personalize hip joint center locations and muscle paths in each model. Model-estimated hip JRFs were projected onto the three-dimensional acetabular rim to predict instantaneous AEL forces and their accumulative impulses throughout a gait cycle. Compared to controls, subjects with DDH demonstrated significantly higher AEL in the antero-superior acetabulum during early stance (3.6 vs. 2.8 × BW, ≤ 0.01), late stance (4.3 vs. 3.3 × BW, ≤ 0.05), and throughout the gait cycle (1.8 vs. 1.4 × BWs, ≤ 0.02), despite having similar hip movement patterns. Elevated AEL primarily occurred in regions where the shallow acetabular edge was in close proximity to the hip JRF direction, and was strongly correlated with the radiographic severity of acetabular deformities. The results suggest AEL is highly dependent on movement and muscle-induced joint loading, and significantly elevated by the DDH acetabular deformities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fspor.2021.687419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281296PMC
July 2021

Altered Small-World Functional Network Topology in Patients with Optic Neuritis: A Resting-State fMRI Study.

Dis Markers 2021 14;2021:9948751. Epub 2021 Jun 14.

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, The Key Laboratory of Neuro-informatics & Rehabilitation Engineering of Ministry of Civil Affairs, Xi'an, Shaanxi 710049, China.

Aim: This study investigated changes in small-world topology and brain functional connectivity in patients with optic neuritis (ON) by resting-state functional magnetic resonance imaging (rs-fMRI) and based on graph theory.

Methods: A total of 21 patients with ON (8 males and 13 females) and 21 matched healthy control subjects (8 males and 13 females) were enrolled and underwent rs-fMRI. Data were preprocessed and the brain was divided into 116 regions of interest. Small-world network parameters and area under the integral curve (AUC) were calculated from pairwise brain interval correlation coefficients. Differences in brain network parameter AUCs between the 2 groups were evaluated with the independent sample -test, and changes in brain connection strength between ON patients and control subjects were assessed by network-based statistical analysis.

Results: In the sparsity range from 0.08 to 0.48, both groups exhibited small-world attributes. Compared to the control group, global network efficiency, normalized clustering coefficient, and small-world value were higher whereas the clustering coefficient value was lower in ON patients. There were no differences in characteristic path length, local network efficiency, and normalized characteristic path length between groups. In addition, ON patients had lower brain functional connectivity strength among the rolandic operculum, medial superior frontal gyrus, insula, median cingulate and paracingulate gyri, amygdala, superior parietal gyrus, inferior parietal gyrus, supramarginal gyrus, angular gyrus, lenticular nucleus, pallidum, superior temporal gyrus, and cerebellum compared to the control group ( < 0.05).

Conclusion: Patients with ON show typical "small world" topology that differed from that detected in HC brain networks. The brain network in ON has a small-world attribute but shows reduced and abnormal connectivity compared to normal subjects and likely causes symptoms of cognitive impairment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/9948751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219459PMC
June 2021

Role of polystyrene microplastics in sunlight-mediated transformation of silver in aquatic environments: Mechanisms, kinetics and toxicity.

J Hazard Mater 2021 Jun 21;419:126429. Epub 2021 Jun 21.

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; China University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Sunlight-oxidative ageing is a common and critical process for microplastics (MPs) in aquatic environments. O, O, and •OH generation has been widely proven in this process, which can alter metal speciation based on its reduction and oxidation potential. Herein, chemical speciation of Ag mediated by polystyrene (PS) MPs was determined under simulated sunlight irradiation. The O generation on the PS MPs surfaces is the vital factor for Ag reduction, regardless of acid or base conditions. The O and •OH are dominant factors, and O played a more important role than •OH for its higher formation amount, causing oxidative dissolution of newly formed Ag nanoparticles (NPs). The Ag NPs can hetero-aggregate with PS MPs through electrostatic interactions with O-containing groups (C-O, C-OH and CO), and co-precipitate from the water phase. This hetero-aggregation can stabilize Ag NPs by inhibiting Ag NPs surface photooxidation and suppressing Ag release. Transformation of Ag species (from Ag to Ag NPs) mediated by sunlight with PS MPs significantly suppressed acute toxicity of Ag to Escherichia coli, Selenastrum capricornutum, Daphnia magna and zebrafish. This study emphasized that PS MPs play an important role in the speciation, migration and toxicity of Ag in freshwater environments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.126429DOI Listing
June 2021

Silencing of Sulfoquinovosyl Diacylglycerol Synthase 1 Impairs the Glycolipids Accumulation and Photosynthesis in Phosphate-deprived Rice.

J Exp Bot 2021 Jun 24. Epub 2021 Jun 24.

Institute of Eco-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Phosphate (Pi) starved crops utilize the phospholipids as a source for internal Pi supply by replacing the non-phosphorus glycolipids. In rice, sulfoquinovosyl diacylglycerol synthase 1 (OsSQD1) functions as a key enzyme to catalyse SQDG formation. In this study, differential expression of OsSQD1 in response to Pi, nitrogen (N), potassium (K) and iron (Fe)-deficiencies in different durations was measured. A electrophoretic mobility shift assay (EMSA) suggests that OsSQD1 is regulated by OsPHR2, an MYB domain-containing transcription factor. The analyses of different lipid species concentrations in a ossqd1-knockout mutant demonstrated that OsSQD1 silencing increased the phospholipid contents and altered the fatty acid composition under Pi-deficiency. Moreover, OsSQD1 silencing reduces glycolipid accumulation under -Pi condition. It also triggered the saturation of fatty acid in phospholipids and glycolipids treated with different Pi regimes. Relative amounts of transcripts related to phospholipid degradation and glycolipid synthesis was assessed to explore the mechanism by which OsSQD1 exerts an effect on lipid homeostasis under -P conditions. Furthermore, OsSQD1 silencing inhibited photosynthesis, especially under Pi-deficient conditions, by down-regulating glycolipids in rice shoots. The study reveals that OsSQD1 plays a key role in lipid homeostasis, especially the glycolipids accumulation under Pi-deficiency, which results in the inhibition of photosynthesis responding to Pi-deficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jxb/erab300DOI Listing
June 2021

Microparticles and microplastics released from daily use of plastic feeding and water bottles and plastic injectors: potential risks to infants and children in China.

Environ Sci Pollut Res Int 2021 Jun 19. Epub 2021 Jun 19.

Collaborative Innovation Center of Water Security for Water Source Region of the Midline of the South-to-North Diversion Project of Henan Province, Nanyang Normal University, Nanyang, 473061, China.

Daily use of plastic feeding and water bottles occur widely in China, and they could be sources for release of microplastics (MPs), which threaten the health of Chinese infants and children during daily usage. In this work, we investigated the use of polycarbonate (PC) and polypropylene (PP) for making water bottles (WBs) and polyphenylene sulfone resins (PPSU) for making feeding bottles (FBs), and we found that feeding bottles and water bottles released microparticles in amounts ranging from 53 to 393 particles/mL during 100 opening/closing cycles. The good linear regressions for plots of microparticles released vs. abrasion distance (r = 0.811) indicated that thick-necked bottles release more microparticles than thin-necked bottles. The brands and types of bottles (plastic vs. glass) influence microparticle release, and this indicates that high-quality plastic and glass bottles release fewer microparticles and are good for the health of infants and children. In addition to calcium stearate and silicone additives, the identified MPs account for 7.5 to 42.1% of released microparticles with different polymer types, sizes (from 20 to 500 μm) and shapes (cubic, spherical and irregular shapes). Additionally, an average of 1.74 MPs were released from an injection with a single-use plastic injector. Nevertheless, a number of microparticles and nanosized plastics were observed with all samples, suggesting that the health risks of micro- and nanosized particles to humans, especially babies and children, and the environment should be considered seriously.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-14939-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214457PMC
June 2021

Efficiency Analysis of New Rural Cooperative Medical System in China: Implications for the COVID-19 Era.

Front Psychol 2021 28;12:686954. Epub 2021 May 28.

School of Economics and Management, Hunan University of Science and Engineering, Yongzhou, China.

The sudden outbreak of coronavirus disease 2019 (COVID-19) has caused a huge impact on the Chinese residents' health and economic level. In the pandemic background, the country and its institutions have introduced pandemic-related insurance to stabilize the national situation. At this stage, insurance has played an increasingly important role in social life. With the popularization of insurance, the idea of buying insurance to avoid risk has gradually become popular among people. Among them, the New Rural Cooperative Medical System (NRCMS) has been farmers' common choice. The NRCMS, a mutual aid system created by farmers spontaneously in the country, plays a great role in guaranteeing farmers access to basic health services, alleviating poverty caused by disease and returning to poverty due to disease, and promoting poverty alleviation and rural revitalization. Given this backdrop, we study the efficiency of the NRCMS that can effectively promote poverty alleviation and rural revitalization and ensure the people's happy life. Implementing the Data Envelopment Analysis (DEA), we find that technological progress is one of the main factors influencing the efficiency of the NRCMS. Therefore, it is important to improve the technology for providing the efficiency of the NRCMS and promoting the happiness of the society.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2021.686954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193048PMC
May 2021

Incidence and Distribution of the Pathogens Causing Central Nervous System Infections at the University Hospital of Korea.

Clin Lab 2021 Jun;67(6)

Background: The pathogens involved in central nervous system (CNS) infections are various, such as viruses, bacteria, and fungi, so a syndromic approach can be required. In addition, since their rapid and accurate detection is very crucial, molecular diagnostics using cerebrospinal fluid is becoming the emerging standard method.

Methods: The study was conducted retrospectively to identify the incidence and distribution patterns of the pathogens according to gender, age, season, and month and to analyze their codetection from August 2017 to July 2020. It was also conducted to investigate turn-around times (TATs) according to the detection method. The detection methods were FilmArray® Meningitis/Encephalitis (M/E) method (FilmArray), Cepheid® Xpert EV assay (Xpert), and Multiplex PCR method for five species of bacteria.

Results: The overall incidence for at least one pathogen was 13.9% (346/2,496). The highest incidence was shown in age group 4 (3 - 6 years), with 27.4%. The detection rates by FilmArray, Xpert, and Multiplex PCR method were 39.8%, 41.7%, and 0.4%, respectively. Enterovirus (EV) showed the highest incidence rate, which accounted for 37.0%. The distribution of the pathogens according to the age groups were the highest in age group 4, with 47.5% (168/354), followed by 27.4% (97/354) in age group 5. Of the ten cases in which bacteria were detected, S. agalactiae accounted for 60.0% (6/10), most of which occurred in age group 1. E. coli K1, L. monocytogenes, and N. meningitidis were not detected. In the viral distribution, EV accounted for the highest proportion in all age groups. The overall proportion of EV accounted for 87.6% (310/354), followed by human parechovirus with 2.8% (10/354). The most commonly detected season was summer, comprising 75.1%. A total of eight cases of co-detection with two pathogens accounted for 1.6% (8/507) in FilmArray. In FilmArray, all TATs were found to be shorter than Xpert.

Conclusions: The information on the incidence and distribution patterns of the pathogens causing CNS infections and their rapid detection are critically important to clinicians in the management of immunocompromised patients, elderly, and children. The expeditious molecular diagnostics for these pathogens would be valuable in medical decisions by clinicians.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7754/Clin.Lab.2020.201118DOI Listing
June 2021

The biomechanical influence of facet joint parameters on corresponding segment in the lumbar spine: a new visualization method.

Spine J 2021 May 30. Epub 2021 May 30.

Department of Orthopaedics, First Affiliated Hospital of Dalian Medical University, No. 5 Longbin Road, Dalian 116600,China; Key Laboratory of Molecular Mechanism for Repair and Remodeling of Orthopaedic Diseases, No. 5 Longbin Road, Dalian 116600,China. Electronic address:

Background Context: Facet joints have been discussed as influential factors in the development of lumbar degeneration, which includes disc herniation and degenerative lumbar spondylolisthesis. Facet orientation (FO) and facet tropism (FT) are two important structural parameters of the lumbar facet joints. Many previous studies have focused on single parameter analysis of the lumbar spine. Owing to the correlation between independent variables, single-factor analysis cannot reflect the interaction between variables; however, there has been no corresponding biomechanical method developed to address this problem.

Purpose: To investigate the complex biomechanical influences on the lumbar spine when vertebral FO and FT are varied using finite element analysis (FEA) and contour maps visualization, and analyze the biomechanical role of facet joint structural parameters in the process of lumbar degenerative diseases.

Study Design: A biomechanical modelling, analysis, and verification study was performed.

Methods: A three-dimensional non-linear FEA model of 3 denucleated intervertebral discs (L2-3, L3-4, L4-5) with adjacent vertebral bodies (L2-L5) was created. Previously performed in vitro experiments provided experimental data for the range of motion in each load direction that was used for calibration. For 12 lumbar models, different facet joint angles relative to the sagittal plane at both L3-4 facet joints were simulated for 35°≤FO≤50° and 0°≤FT≤15°. By modifying different values of FO and FT, FEA simulation of different lumbar spine models was performed. Contour maps were used to visualize the FO- and FT-relevant data.

Results: Under flexion, extension, and torsion moments, facet joint contact force and intradiscal stress increased with increasing FT. In the condition where FT remained 0° and increasing FO values, facet joint contact force and intradiscal stress remained low with no apparent increasing or decreasing trend when the model was under flexion, extension, and torsion moments. In the condition where FO and the FT values were varied at the same time, the highest force and stress regions in the contour maps were observed when all three types of moments were applied. Stress distributions of the L3-4 disc with different FT and FO values showed disc stress increased significantly with increases of FT and was concentrated on the ipsilateral region of the facet joint with the more sagittal orientation.

Conclusions: The combination of FO and FT has an important impact on the corresponding disc and facet joints, but FT played a more significant role. Moreover, disc stress was concentrated on the ipsilateral region of facet joint with greater sagittal orientation when FT existed. FT with high sagittal orientation may increase risk of recurrent LDH due to increase ipsilateral disc pressure.

Clinical Significance: These biomechanical findings may help clinicians to understand the prognosis of some lumbar degenerative conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.spinee.2021.05.024DOI Listing
May 2021

Influence of elemental sulfur on cadmium bioavailability, microbial community in paddy soil and Cd accumulation in rice plants.

Sci Rep 2021 Jun 1;11(1):11468. Epub 2021 Jun 1.

ECO-Environment Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.

Cadmium (Cd) is highly toxic to living organisms and the contamination of Cd in paddy soil in China has received much attention. In the present study, by conducting pot experiment, the influence of S fertilizer (S) on rice growth, iron plaque formation, Cd accumulation in rice plants and bacterial community in rice rhizosphere soil was investigated. The biomass of rice plants was significantly increased by S addition (19.5-73.6%). The addition of S increased the formation of iron plaque by 24.3-45.8%, meanwhile the amount of Cd sequestered on iron plaque increased. In soil treated with 5 mg/kg Cd, addition of 0.2 g/kg S decreased the diffusive gradients in thin films (DGT) extractable Cd by 60.0%. The application of S significantly decreased the concentration of Cd in rice grain by 12.1% (0.1 g/kg) and 36.6% (0.2 g/kg) respectively. The addition of S significantly increased the ratio of Acidobacteria, Bacteroidetes in rice rhizosphere soil. Meanwhile, the ratio of Planctomycetes and Chloroflexi decreased. The results indicated that promoting Fe- and S-reducing and residue decomposition bacterial in the rhizosphere by S may be one biological reason for reducing Cd risk in the soil-rice system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-91003-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169911PMC
June 2021

Soil bacterial community composition in rice-fish integrated farming systems with different planting years.

Sci Rep 2021 May 25;11(1):10855. Epub 2021 May 25.

Eco-Environmental Protection Institute of Shanghai Academy of Agricultural Sciences, 1000 Jinqi Rd., Shanghai, 201403, People's Republic of China.

The high productivity and efficient nutrient utilization in rice-fish integrated farming system are well reported. However, the characteristics of soil bacterial communities and their relationship with soil nutrient availability in rice-fish field remain unclear. In this study, we selected three paddy fields, including a rice monoculture field and two rice-fish fields with different planting years, to investigate the soil bacterial community composition with Illumina MiSeq sequencing technology. The results indicated that the soil properties were significantly different among different rice farming systems. The soil bacterial community composition in the rice-fish field was significantly different from that in the rice monoculture field. Five of the top 15 phyla were observed with significant differences and Nitrospirae was the most significant one. However, no taxa observed with significance between the rice planting area and aquaculture area no matter in the 1st or 5th year of rice-fish field. RDA analysis showed that the soil bacterial community differentiation in the 5th year of rice-fish field was positively correlated with soil properties, such as AN and OM contents, EC and pH value. Although the rice yields in rice-fish field decreased, the net economic benefit of the rice-fish system enhanced obviously due to the high value of aquaculture animals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-90370-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149657PMC
May 2021

Effects of long-term integrated agri-aquaculture on the soil fungal community structure and function in vegetable fields.

Sci Rep 2021 May 24;11(1):10813. Epub 2021 May 24.

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

The diversity and community structure of soil fungi play an important role in crop production and ecosystem balance, especially in paddy-upland vegetable field systems. High-throughput sequencing was used to study changes in the soil fungal community structure and function in paddy-upland vegetable field systems. The results showed that compared with traditional planting, the diversity and community structure of soil fungi were changed by the combination of flooding and drought, the Shannon index increased by 11.07%, and the proportion of the dominant species, Mortierella, decreased by 22.74%. Soil available nitrogen, total phosphorus, available phosphorus, total nitrogen and organic matter played a leading role in the initial stage of the experiment, while the dominant factor changed to total potassium 3 years later and then to soil pH and water content 6 years later. FUNGuild analysis showed that the proportion of three independent trophic modes of soil fungi were increased by the combined flooded-drought model, and there were multiple interaction factors, For example, nutrient supply, pH and planting pattern. This study showed that soil fertility, crop yield and economic benefits were better than the traditional model after three years of planting and breeding. The longer the time, the better the effect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-90109-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144417PMC
May 2021

Enhanced Electrochemical Properties of Non-stoichiometric Layered Perovskites, SmBaCoO, for IT-SOFC Cathodes.

Front Chem 2021 21;9:633868. Epub 2021 Apr 21.

Department of Advanced Materials Science and Engineering, Hanbat National University, Daejeon, South Korea.

In this study, electrochemical properties of layered perovskites having non-stoichiometric compositions (SmBaCoO, = 0, 0. 01, 0.02, 0.03, 0.04, 0.05, 0.10, and 0.15) were analyzed for the direct application of cathode materials for Intermediate Temperature-operating Solid Oxide Fuel Cells (IT-SOFC). From the SmBaCoO oxide systems calcined at 1,100°C for 8 h, single phase (SmBaCoO, SBCO_1) was maintained only in the case of the = 0 composition. In the compositions of = 0.05-0.10, BaCoO was mixed with the pattern of SBCO. In addition, in the composition of = 0.15, it was confirmed that BaCoO and CoO phases coexisted with SBCO. In the compositions of SmBaCoO, the overall Area Specific Resistance (ASR) values decreased as the removal amount of Sm increased from = 0-0.10; then, the values increased for compositions from = 0.15. For example, the ASRs of SBCO_1, SmBaCoO (SBCO_0.95), SmBaCoO (SBCO_0.90), and SmBaCoO (SBCO_0.85) measured at 600°C were 0.301, 0.147, 0.119, and 0.179 Ω cm, respectively. In particular, SBCO_0.90 was found to have an excellent ASR property of about 0.035 Ω cm at 700°C. Typical properties of the metal-insulator transition (MIT) electrical conductivity were shown in all measured compositions. The temperature at which MIT occurred increased as the non-stoichiometric composition increased.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2021.633868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097098PMC
April 2021

The chromatin accessibility landscape reveals distinct transcriptional regulation in the induction of human primordial germ cell-like cells from pluripotent stem cells.

Stem Cell Reports 2021 May 29;16(5):1245-1261. Epub 2021 Apr 29.

Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China; Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, Guangdong, China; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China; Key Laboratory of Mental Health of the Ministry of Education, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Southern Medical University, Guangzhou, Guangdong, China; Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; National Clinical Research Center for Kidney Disease, Guangzhou, China. Electronic address:

In vitro induction of human primordial germ cell-like cells (hPGCLCs) provides an ideal platform to recapitulate hPGC development. However, the detailed molecular mechanisms regulating the induction of hPGCLCs remain largely uncharacterized. Here, we profiled the chromatin accessibility and transcriptome dynamics throughout the process of hPGCLC induction. Genetic ablation of SOX15 indicated the crucial roles of SOX15 in the maintenance of hPGCLCs. Mechanistically, SOX15 exerted its roles via suppressing somatic gene expression and sustaining latent pluripotency. Notably, ETV5, a downstream regulator of SOX15, was also uncovered to be essential for hPGCLC maintenance. Finally, a stepwise switch of OCT4/SOX2, OCT4/SOX17, and OCT4/SOX15 binding motifs were found to be enriched in closed-to-open regions of human embryonic stem cells, and early- and late-stage hPGCLCs, respectively. Collectively, our data characterized the chromatin accessibility and transcriptome landscapes throughout hPGCLC induction and defined the SOX15-mediated regulatory networks underlying this process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.stemcr.2021.03.032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185471PMC
May 2021

Hormesis effects of gamma radiation on growth of quinoa ().

Int J Radiat Biol 2021 6;97(7):906-915. Epub 2021 May 6.

Department of Agronomy, Gyeongsang National University, Jinju, Korea.

Purpose: Quinoa is an annual plant that grows well in high altitude regions with high radiation and ultraviolet intensity. It has known that high-dose radiation damages living organisms, but low-dose radiation also has a beneficial effect. Therefore, the purpose of this study is to investigate the hormesis effect of gamma-ray on quinoa by growth analysis and hyperspectral imaging.

Materials And Methods: Quinoa seeds were irradiated at 50, 100, and 200 Gy emitted by CO. Subsequently, the seeds were germinated and transplanted into pots, then conducted growth analysis and physiological evaluation every week, and hyperspectral imaging. Photosynthetic ability was measured at 35 days after transplanting (DAT), and the plants for each dose were divided into aerial and underground parts for biomass evaluation at 91 DAT. Various vegetation indices were estimated from 14 to 35 DAT by hyperspectral analysis, and the specific bands were extracted based on the PLS model using plant height, SPAD value, and chlorophyll fluorescence parameters.

Results: We found that plant height and biomass were increased in quinoa plants treated with a low dose (50 Gy) as compared to control. Chlorophyll content and chlorophyll fluorescence were not different between doses at the early growth stage, but as growth progressed, the plant irradiated at 200 Gy began to be lower. The photosynthetic ability of the quinoa plant treated at 50 Gy was greater than other plants at 35 DAT. The vegetation indices related to the pigment status also were higher in the plants treated by irradiation at 50 Gy than the plants grown in other doses treatment units at the beginning of the growth. Using the PLS model we collected sensitive band wavelengths from hyperspectral image analysis. Among the collected bands, eight bands closely related to plant height, nine bands to chlorophyll content, and ten bands to chlorophyll fluorescence were identified.

Conclusion: Our results showed that the growth and physiological parameters of quinoa treated by low dose gamma irradiation to seeds were greater than that of control as well as the plant with higher doses. These findings confirm that the positive changes in the characteristics of quinoa with low dose radiation indicated that hormesis occurs at 50 Gy radiation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/09553002.2021.1919783DOI Listing
May 2021

In vitro and in vivo research of atmosphere pressure nonequilibrium plasmas on root canal disinfection: implication for alternative strategy for irrigation.

Clin Oral Investig 2021 Mar 24. Epub 2021 Mar 24.

Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, Hubei, China.

Objective: To investigate an intracanal disinfection methodology of APNPs (atmosphere pressure nonequilibrium plasmas) or modified APNPs in root canal treatment and evaluate the antimicrobial efficiency against in vitro infected dentinal tubules and in vivo experimental apical periodontitis.

Materials And Methods: Dentine specimens were centrifugated with Enterococcus faecalis to generate 1-day-old and 3-week-old biofilms, and were treated with 2% chlorhexidine (Chx), APNP or modified APNP for 3 and 10 min (n=4). LIVE/DEAD staining was employed to analyze the ratio of deactivated bacteria. Experimental apical periodontitis in beagles was induced. Root canal therapy with APNPs or modified APNPs was performed and the antimicrobial effect was evaluated by histological and radiographical analyses.

Results: APNP deactivated 1-day-old and 3-week-old E. feacalis in dentinal tubules as much as 2% Chx irrigating. Modified APNP significantly deactivated more E. faecalis biofilms in dentinal tubules for 3-min and 10-min treatments, without thermal damage or dentinal destruction being observed. In beagles' apical periodontitis, significantly increased BV/TV and decreased lesion volume of apical bone were found in modified APNP group than 2% Chx irrigation group according to μCT. Fewer inflammatory cells and bacterial residual in dentine were observed in modified APNP-treated apical tissue by histology staining compared with those in the 2% Chx irrigation group.

Conclusion: The antimicrobial effect of APNP jet irradiation was comparable to that of 2% Chx irrigation. No structural damage in dentine or tissue necrosis at the periapical region was induced upon treatment. The modified APNP demonstrated an increased antimicrobial efficacy compared with 2% Chx irrigation both in vitro and in vivo.

Clinical Relevance: The modified APNPs can be used as an alternative intracanal disinfection strategy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00784-021-03888-7DOI Listing
March 2021

Detection of Human Sapoviruses in Sewage in China by Next Generation Sequencing.

Food Environ Virol 2021 06 23;13(2):270-280. Epub 2021 Mar 23.

Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, No. 16992 Jingshi Road, Jinan, 250014, People's Republic of China.

Human sapovirus (SaV) is an important causative agent of nonbacterial gastroenteritis in humans. However, little is known about its circulation in China. To study the prevalence and diversity of human SaV genotypes circulating in eastern China, a 3-year environmental surveillance combined with next generation sequencing (NGS) technology was conducted. A total of 36 raw sewage samples were collected from January 2017 to December 2019 in Jinan and processed. Thirty-five (97.22%) samples were positive for human SaV genome in quantitative RT-PCR assay; 33 (91.67%) samples were positive in nested RT-PCR assay on partial capsid VP1 sequence and all amplicons were further analyzed separately by NGS. Among those, ten genotypes belonging to the genogroups of GI, GII, GIV, and GV were identified by NGS, including 4 major genotypes (GI.2, GI.1, GV.1 and GI.3) and 6 uncommon genotypes (GII.5, GII.1, GII.NA1, GII.3, GI.6 and GIV.1). A temporal switch of predominant genotype was observed from GI.2 to GI.1 around June 2019. Local and foreign sequences clustered together in some branches according to phylogenetic analysis, indicating frequent transmission of various lineages in different regions of the world. Environmental surveillance provides a comprehensive picture of human SaV in China. NGS-based environmental surveillance improves our knowledge on human SaV circulating in communities greatly and should be encouraged as a sensitive surveillance tool.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12560-021-09469-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985922PMC
June 2021

SmBaCaCoO Layered Perovskite Cathodes for Intermediate Temperature-operating Solid Oxide Fuel Cells.

Front Chem 2020 25;8:628813. Epub 2021 Jan 25.

Department of Advanced Materials Science and Engineering, Hanbat National University, Daejeon, South Korea.

In SmBaCaCoO (x = 0.01, 0.03, 0.1, and 0.2, SBCCO) oxide systems calcined at 1100°C for 8 h, the XRD patterns of the SBCCO single phase were maintained in the cases of SmBaCaCoO (SBCCO-0.97) and SmBaCaCoO (SBCCO-0.99) compositions. In SmBaCaCoO (SBCCO-0.8) and SmBaCaCoO (SBCCO-0.9), CaCoSmO existed with the pattern SBCCO. SBCCO structures were identified as orthorhombic crystal structures because they showed splitting of the X-ray diffraction (XRD) peaks at 23.4°, 47.9°, and 59.1°.Typical metallic conduction behaviors were found in all measured compositions except SBCCO-0.8, which showed a metal-insulator transition (MIT) behavior. Compared to other SmBaCaCoO compositions, SBCCO-0.8 showed the highest electrical conductivity of 460 S/cm at 500°C. In particular, SBCCO-0.9 was found to have an excellent ASR characteristic of about 0.077 Ωcm at 700°C. The activation energy of SBCCO-0.9 was the lowest among SBCCO oxide systems with a value of 0.77 eV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2020.628813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958919PMC
January 2021

Impact of the 2019 Novel Coronavirus Disease Pandemic on the Performance of a Cardiovascular Department in a Non-epidemic Center in Beijing, China.

Front Cardiovasc Med 2021 18;8:630816. Epub 2021 Feb 18.

Department of Cardiology and Macrovascular Disease, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Knowledge of the impact of the 2019 novel coronavirus disease (COVID-19) pandemic on the performance of a cardiovascular department in a medical referral hub center from a non-epidemic area of China is limited. The data on the total number of non-emergency medical cares (including the number of out-patient clinic attendances, the number of patients who were hospitalized in non-intensive care wards, and patients who underwent elective cardiac intervention procedures) and emergency medical cares [including the number of emergency department (ED attendances) and chest pain center (CPC attendances), as well as the number of patients who were hospitalized in coronary care unit (CCU) and the number of patients who underwent emergency cardiac intervention procedures] before and during the pandemic (time before the pandemic: 20th January 2019 to 31st March 2019 and time during the pandemic: 20th January 2020 to 31st March 2020) in the Department of Cardiology and Macrovascular Disease, Beijing Tiantan Hospital, Capital Medical University were collected and compared. Both the non-emergency medical and emergency medical cares were affected by the pandemic. The total number of out-patient clinic attendance decreased by 44.8% and the total number of patients who were hospitalized in non-intensive care wards decreased by 56.4%. Pearson correlation analysis showed that the number of out-patient clinic attendance per day was not associated with the number of new confirmed COVID-19 cases and the cumulative number of confirmed COVID-19 patients in Beijing ( = -0.080, = 0.506 and = -0.071, = 0.552, respectively). The total number of patients who underwent non-emergency cardiac intervention procedures decreased during the pandemic, although there were no statistically significant differences except for patent foramen ovale (PFO) occlusion (1.7 ± 2.9 vs. 8.3 ± 2.3, = 0.035). As for the emergency medical cares, the ED attendances decreased by 22.4%, the total number of CPC attendances increased by 10.3%, and the number of patients who were hospitalized in CCU increased by 8.9%: these differences were not statistically significant. During the pandemic, the proportion of hospitalized patients with ST segment elevation myocardial infarction (STEMI) and non-ST segment elevation myocardial infarction (NSTEMI) significantly increased (19.0 vs. 8.7%, < 0.001; 28.8 vs. 18.0%, < 0.001, respectively); also, the number of primary percutaneous coronary intervention (PCI) increased by 10.3%. There was no significant difference between patients before and during the pandemic regarding the age, gender, baseline and discharge medication therapy, as well as length of stay and in-hospital mortality. Our preliminary results demonstrate that both the non-emergency and emergency medical cares were affected by the COVID-19 pandemic even in a referral medical center with low cross-infection risk. The number of the out-patient clinic attendances not associated with the number of confirmed COVID-19 cases could be due to different factors, such as the local government contamination measures. The proportion of hospitalized patients with acute myocardial infarction increased in our center during the pandemic since other hospitals stopped performing primary angioplasty. A hub-and-spoke model could be effective in limiting the collateral damage for patients affected by cardiovascular diseases when the medical system is stressed by disasters, such as COVID-19 pandemic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2021.630816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7929980PMC
February 2021

WIPI1 promotes osteosarcoma cell proliferation by inhibiting CDKN1A.

Gene 2021 May 23;782:145537. Epub 2021 Feb 23.

Orthopeadic Dept. Xinqiao Hospital, Army Medical University, Chongqing, China. Electronic address:

Detection of TCGA data revealed that WIPI1 is highly expressed in osteosarcoma cells. So we explore the mechanisms of WIPI1 affecting the proliferation of osteosarcoma cells through Affymetrix microarray analysis. Functional analysis of differentially expressed genes shows that the classical signaling pathways affecting tumor formation and development have changed significantly. By fitting analysis, it is speculated that the WIPI1 may function in the direction of osteosarcoma by regulating the expression of multiple cell cycle-related genes such as CDKN1A, CDK4 and CCND1. Therefore, the key genes are selected for RT-PCR and Western-blot verification. Combined with flow and other means, WIPI1 may affect the cell cycle and the osteosarcoma by regulating the expression of CDKN1A, CDK4 and CCND1. To verify the results, the effect of WIPI1 on cell proliferation was quantified by MTT, cell counts and nude mouse tumorigenicity assay. The results showed that WIPI1 promotes osteosarcoma cell proliferation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gene.2021.145537DOI Listing
May 2021

The Effects of Earthworms on Fungal Diversity and Community Structure in Farmland Soil With Returned Straw.

Front Microbiol 2020 17;11:594265. Epub 2020 Dec 17.

Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Background: To promote the decomposition of returned straw, reduce the incidence of soil-borne diseases caused by returned straw, and accelerate the conversion of straw carbon into soil carbon, we inoculated earthworms into fields with returned straw. The earthworms accelerated straw degradation and promoted carbon conversion. However, the impact of externally inoculated earthworms on the farmland soil ecosystem, especially the structure and the function of its microbial community, remains unclear.

Methods: We analyzed the effects of straw return and earthworms on the diversity of fungal populations and the community structure of dominant fungal taxa in soil by quantifying fungal population size and community composition PCR amplification of internal transcribed spacer genes and 18S rRNA gene sequencing.

Results: The results showed that earthworm inoculation significantly accelerated the degradation of rice straw and promoted the conversion of straw carbon to soil carbon. Both fungal abundance and α-diversity (Sobs and Shannon indices) were higher in the plots with surface straw but without earthworms than in those inoculated with earthworms and in the CK. Principal component analysis indicated that straw return increased the diversity and the abundance of the fungal community, whereas earthworms inhibited this expansion of the fungal community caused by straw return. Interestingly, the overall differences in fungal community composition were smallest in plots with straw return, while the dominant fungal community features in plots inoculated with earthworms were closer to those of the CK.

Conclusion: Generally, straw return stimulated unclassified_K_fungi, , and with strong cellulolytic ability. In contrast, the abundances of , unclassified_c_Sordariomycetes, unclassified_f_Lasiosphaeriaceae, and were higher in the plots inoculated with earthworms and in the CK. Furthermore, evolutionary analysis showed that the evolution of soil fungal communities tended to diverge after straw return, and the evolutionary directions of fungal species in the plots inoculated with earthworms were similar to those in the CK.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2020.594265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773728PMC
December 2020

Indirubin-3'-Oxime (IDR3O) Inhibits Proliferation of Osteosarcoma Cells in vitro and Tumor Growth in vivo Through AMPK-Activation and PGC-1α/TFAM Up-Regulation.

Dokl Biochem Biophys 2020 Nov 25;495(1):354-360. Epub 2020 Dec 25.

Department of Traumatology, Dezhou People's Hospital, 253000, Dezhou, China.

Osteosarcoma, a malignant tumor of bones, has very high incidence in adolescents and young people. The present study investigated the effect of indirubin-3'-oxime (IDR3O) derivative on proliferation of osteosarcoma cells in vitro and tumor growth in vivo. Changes in growth and induction of apoptosis in osteosarcoma cells were assessed using WST-8 and TUNEL staining assays. Treatment of MG63 and Saos‑2 cells with IDR3O inhibited proliferation, activated apoptosis and promoted AMPK-activation. In IDR3O treated MG63 and Saos‑2 cells PGC-1α (Peroxisome proliferator-activated receptor-γ coactivator-1α) levels were markedly promoted compared to control (untreated) cells. In the mice model osteosarcoma was induced by implantation of 2 × 10 MG63 cells on dorsal side subcutaneously. Then the experimental group of mice received IDR3O intra-peritoneally during 45 days. IDR3O-treatment suppressed tumor development significantly compared to control (untreated) group but didn't changed body weight. IDR3O inhibits osteosarcoma cell growth and activates apoptosis through AMPK dependent pathway. Therefore, IDR3O may be considered for treatment of osteosarcoma as it effectively arrests tumor growth in mice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1134/S1607672920060022DOI Listing
November 2020

Incidence and Distribution of Respiratory Microorganisms Causing Acute Respiratory Infections at the University Hospital of Korea.

Clin Lab 2020 Dec;66(12)

Background: Acute respiratory infection caused by respiratory microorganisms including various kinds of viruses and bacteria is the most common infectious disease. When managing patients, it is crucial to detect these microorganisms rapidly and monitor their occurrence and tendency. Recently, the methods of detecting them have been implemented by molecular diagnostics. The authors intended to investigate their incidence and distribution and identify the significance of the molecular diagnosis for their detection.

Methods: The retrospective study was conducted to investigate the incidence and distribution of respiratory microorganisms according to the age, gender, month, season, and the detection method and to analyze their co-infections from July 2016 to December 2019. In addition, the four types of turn-around time (TAT) for each detec-tion method were also analyzed.

Results: The overall incidence for at least one respiratory microorganism was 23.1% (3,645/15,808). The highest incidence was identified in age group 2 (1 - 3 months), 38.5%. The incidence rates by multiplex PCR using Anyplex and Allplex, FilmArray method, and influenza virus (flu) antigen detection test were 44.2% (718/1,625), 63.1% (1,198/1,899), and 14.1% (1,729/12,284), respectively. The overall incidence between male and female patients showed no statistically significant difference (p = 0.980), except for the flu antigen detection test (p = 0.000). Influenza A viruses (flu A) accounted for the highest percentage (34.9%), followed by rhinovirus/enterovirus (20.5%), RSV (12.8%), flu B (8.3%), and adenovirus (7.6%). These microorganisms showed characteristic distribution patterns according to season and month. Flu A and flu B predominated in winter and accounted for an increasing proportion as age increased according to the age groups. The overall co-infection rate was 22.5% (432/1,916). The average TATs of the FilmArray method were significantly much faster than multiplex PCR using Anyplex and Allplex (p = 0.000).

Conclusions: The information on the incidence and distribution of respiratory microorganisms and their expeditious detection are considered critical to the management of the elderly, immunocompromised patients, and children. The rapid molecular-based diagnosis of respiratory infections would be beneficial in medical decision and prevention of their propagation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7754/Clin.Lab.2020.200441DOI Listing
December 2020

Effect of Platelet-Rich Plasma on Intervertebral Disc Degeneration and : A Critical Review.

Oxid Med Cell Longev 2020 21;2020:8893819. Epub 2020 Nov 21.

Department of Orthopaedics, First Affiliated Hospital of Dalian Medical University, Dalian, China.

Intervertebral disc degeneration (IDD) is a globally occurring disease that represents a significant cause of socioeconomic problems. Currently, the main method for treating IDD is surgery, including discectomy and vertebral fusion. Several experiments demonstrated that platelet-rich plasma (PRP) could stimulate cell proliferation and extracellular matrix regeneration. Additionally, experiments have proven that PRP injection could restore intervertebral disc height. Clinical studies demonstrated that PRP injection could significantly relieve patient pain. However, further studies are still required to clarify the roles of PRP in IDD prevention and treatment. This review is aimed at summarizing and critically analyzing the current evidence regarding IDD treatment with PRP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/8893819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7704139PMC
June 2021

Electronic Health Record Reminder Effect on Hepatitis C Antibody Screening.

J Am Board Fam Med 2020 Nov-Dec;33(6):1016-1019

From the Department of Family Medicine and Community Health, William Beaumont School of Medicine, Oakland University, Sterling Heights, MI (EM, IB); Research Institute, Beaumont Hospital, Royal Oak, MI (KS).

In the United States, national guidelines recommend screening adults born between 1945 and 1965 for hepatitis C, but screening rates in this population continue to be low. We added a hepatitis C screening reminder to the Epic Electronic Health Record and educated physicians on the use of the Health Maintenance section in Epic. We assessed the effect of this intervention on the completion of screening hepatitis C antibody tests. We examined data from 2 years before and after the addition of the reminder. Completed hepatitis C antibody testing increased from 733 to 6502, and the rate of positive testing decreased from 5.9% to 2.0%. Implementing the electronic health record reminder and educating providers on the routine use of the Health Maintenance section increased hepatitis C screening for at risk adults.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3122/jabfm.2020.06.190440DOI Listing
November 2019

Cardiac CaMKII and Wenxin Keli Prevents Ang II-Induced Cardiomyocyte Hypertrophy by Modulating CnA-NFATc4 and Inflammatory Signaling Pathways in H9c2 Cells.

Evid Based Complement Alternat Med 2020 19;2020:9502651. Epub 2020 Oct 19.

Guang'anmen Hospital, Chinese Academy of Chinese Medical Sciences, Beijing 100053, China.

Previous studies have demonstrated that calcium-/calmodulin-dependent protein kinase II (CaMKII) and calcineurin A-nuclear factor of activated T-cell (CnA-NFAT) signaling pathways play key roles in cardiac hypertrophy (CH). However, the interaction between CaMKII and CnA-NFAT signaling remains unclear. H9c2 cells were cultured and treated with angiotensin II (Ang II) with or without silenced CaMKII (siCaMKII) and cyclosporine A (CsA, a calcineurin inhibitor) and subsequently treated with Wenxin Keli (WXKL). Patch clamp recording was conducted to assess L-type Ca current (I), and the expression of proteins involved in signaling pathways was measured by western blotting. Myocardial cytoskeletal protein and nuclear translocation of target proteins were assessed by immunofluorescence. The results indicated that siCaMKII suppressed Ang II-induced CH, as evidenced by reduced cell surface area and I. Notably, siCaMKII inhibited Ang II-induced activation of CnA and NFATc4 nuclear transfer. Inflammatory signaling was inhibited by siCaMKII and WXKL. Interestingly, CsA inhibited CnA-NFAT pathway expression but activated CaMKII signaling. In conclusion, siCaMKII may improve CH, possibly by blocking CnA-NFAT and MyD88 signaling, and WXKL has a similar effect. These data suggest that inhibiting CaMKII, but not CnA, may be a promising approach to attenuate CH and arrhythmia progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/9502651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603598PMC
October 2020

PDGFRA in vascular adventitial MSCs promotes neointima formation in arteriovenous fistula in chronic kidney disease.

JCI Insight 2020 11 5;5(21). Epub 2020 Nov 5.

Selzman Institute for Kidney Health, Section of Nephrology, Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.

Chronic kidney disease (CKD) induces the failure of arteriovenous fistulas (AVFs) and promotes the differentiation of vascular adventitial GLI1-positive mesenchymal stem cells (GMCs). However, the roles of GMCs in forming neointima in AVFs remain unknown. GMCs isolated from CKD mice showed increased potential capacity of differentiation into myofibroblast-like cells. Increased activation of expression of PDGFRA and hedgehog (HH) signaling were detected in adventitial cells of AVFs from patients with end-stage kidney disease and CKD mice. PDGFRA was translocated and accumulated in early endosome when sonic hedgehog was overexpressed. In endosome, PDGFRA-mediated activation of TGFB1/SMAD signaling promoted the differentiation of GMCs into myofibroblasts, extracellular matrix deposition, and vascular fibrosis. These responses resulted in neointima formation and AVF failure. KO of Pdgfra or inhibition of HH signaling in GMCs suppressed the differentiation of GMCs into myofibroblasts. In vivo, specific KO of Pdgfra inhibited GMC activation and vascular fibrosis, resulting in suppression of neointima formation and improvement of AVF patency despite CKD. Our findings could yield strategies for maintaining AVF functions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1172/jci.insight.137298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710276PMC
November 2020

Earthworms accelerate rice straw decomposition and maintenance of soil organic carbon dynamics in rice agroecosystems.

PeerJ 2020 17;8:e9870. Epub 2020 Sep 17.

Institute of Eco-Environmental and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Background: To promote straw degradation, we inoculated returned farmland straw with earthworms (). Increasing the number of earthworms may generally alter soil organic carbon (SOC) dynamics and the biological activity of agricultural soils.

Methods: We performed soil mesocosm experiments with and without earthworms to assess the decomposition and microbial mineralization of returned straw and soil enzyme activity across different time periods.

Results: When earthworms were present in soil, the surface residues were completely consumed during the first four weeks, but when earthworms were absent, most of the residues remained on the soil surface after 18 weeks. On day 28, the SOC content was significantly higher in the treatment where both earthworms and residue had been added. The SOC content was lower in the treatment where earthworms but no residue had been added. The organic carbon content in water-stable macroaggregates showed the same trend. During the first 14 weeks, the soil basal respiration was highest in the treatments with both residues and earthworms. From weeks 14 to 18, basal respiration was highest in the treatments with residues but without earthworms. We found a significant positive correlation between soil basal respiration and soil dissolved organic carbon content. Earthworms increased the activity of protease, invertase, urease and alkaline phosphatase enzymes, but decreased β-cellobiohydrolase, β-glucosidase and xylosidase activity, as well as significantly reducing ergosterol content.

Conclusion: The primary decomposition of exogenous rice residues was mainly performed by earthworms. Over a short period of time, they converted plant carbon into soil carbon and increased SOC. The earthworms played a key role in carbon conversion and stabilization. In the absence of exogenous residues, earthworm activity accelerated the decomposition of original organic carbon in the soil, reduced SOC, and promoted carbon mineralization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.9870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7502234PMC
September 2020

Hidden blood loss and its possible risk factors in minimally invasive transforaminal lumbar interbody fusion.

J Orthop Surg Res 2020 Sep 29;15(1):445. Epub 2020 Sep 29.

Department of Orthopaedics, First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China.

Background: With respect to spinal surgeries, elucidating absolute and relative amount of hidden blood loss (HBL) is of great importance in order to avoid aforementioned potential complications. To evaluate HBL and its possible risk factors among patients undergoing minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) for lumbar degenerative diseases.

Methods: Between June 2018 and March 2019, 137 consecutive patients with lumbar degenerative disease, who underwent operation with MIS-TLIF technique, were enrolled in this study. The patient's demographic characteristics and blood loss-related parameters were collected, respectively. The Pearson or Spearman correlation analysis was used to investigate an association between patient's characteristics and HBL. Multivariate linear regression analysis was used to confirm independent risk factors of HBL.

Results: A total of 137 patients (86 males and 51 females, age range 19-78 years) were reviewed in our hospital. A substantial amount of HBL (488.4 ± 294.0 ml, 52.5% of TBL) occurred after MIS-TLIF. Multivariate linear regression showed that the age, muscle thickness, the Patients' Society of Anesthesiologists (ASA) classification, patient's blood volume (PBV), total blood loss (TBL), postoperative (i.e., day 2 or 3) hematocrit (Hct), Hct loss, and fibrinogen level were independent risk factors for HBL (P1 = 0.000, P2 = 0.002, P3 = 0.006, P4 = 0.002, P5 = 0.003, P6 = 0.048, P7 = 0.004, P8 = 0.000).

Conclusion: A large amount of HBL was incurred in patients undergoing MIS-TLIF. More importantly, the age, muscle thickness, ASA classification, PBV, TBL, postoperative Hct, Hct loss, and fibrinogen level were independent risk factors for HBL in MIS-TLIF. HBL and its risk factors should be paid more attention to during the perioperative period.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-020-01971-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7525988PMC
September 2020

Comparison of Clinical Outcomes in Patients with ST Elevation Myocardial Infarction with Percutaneous Coronary Intervention and the Use of a Telemedicine App Before and After the COVID-19 Pandemic at a Center in Beijing, China, from August 2019 to March 2020.

Med Sci Monit 2020 Sep 17;26:e927061. Epub 2020 Sep 17.

Department of Cardiology and Macrovascular Disease, Beijing Tiantan Hospital, Capital Medical University, Beijing, China (mainland).

BACKGROUND The efficacy of telemedicine in reducing delay times and short-term adverse clinical outcomes in patients with ST segment elevation myocardial infarction (STEMI) during the coronavirus disease 2019 (COVID-19) pandemic is unclear. This study compared outcomes in patients with STEMI who had percutaneous coronary intervention (PCI) and the use of a telemedicine app from August 2019 to March 2020 at a single center in Beijing, China. MATERIAL AND METHODS A total of 243 patients with STEMI who underwent PCI were consecutively enrolled and divided into 2 groups according to the date, before or after the pandemic. The 2 groups were further divided into patients who used the app for consulting and those who did not. RESULTS The time from symptom onset to calling an ambulance (SCT), door to balloon time (DTB), and total ischemia time (TIT) were significantly prolonged in patients after the pandemic. Patients who used the app had shorter SCT, DTB, and TIT before and after the pandemic compared to those who did not. Adverse clinical outcomes were significantly higher after compared with before the pandemic, despite the incidence rate of stroke, any revascularization, and stent thrombosis. However, there was no significant difference in short-term adverse clinical outcomes between patients who used the app and those who did not before and after the pandemic. CONCLUSIONS Telemedicine reduced the delay time of STEMI patients during the COVID-19 pandemic. The difference in short-term adverse clinical outcomes was not statistically significant between patients who used the app and those who did not.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12659/MSM.927061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521072PMC
September 2020
-->