Publications by authors named "Song Han"

537 Publications

The role of Mfn2 in the structure and function of ER-mitochondrial tethering in vivo.

J Cell Sci 2021 Jun 10. Epub 2021 Jun 10.

Department of Pathology, Case Western Reserve University, Cleveland, OH, USA.

The mitochondria-ER contacts (MERCs) plays an essential role in multiple cell physiological process. While Mfn2 was the first protein implicated in the formation of MERCs, it is debated whether it acts as a tether or antagonizer, largely based on in vitro studies. To understand the role of Mfn2 in MERCs in vivo, we characterized ultrastructural and biochemical changes of MERCs in pyramidal neurons of hippocampus in Mfn2 conditional knockout (KO) mice and in Mfn2 overexpression (OE) mice and found Mfn2 ablation caused reduced close contacts while Mfn2 OE caused increased close contacts between ER and mitochondria in vivo. Functional studies on SH-SY5Y cells with Mfn2 KO or overexpression demonstrating similar biochemical changes found that mitochondrial calcium uptake along with IP3R3-Grp75 interaction was decreased in Mfn2 KO cells but increased in the Mfn2 OE cells. Lastly, we found Mfn2 KO decreased and Mfn2 OE increased the interaction between the ER-mitochondria tethering pair of VAPB-PTPIP51. In conclusion, our study supports the notion that Mfn2 plays a critical role in ER-mitochondrial tethering and the formation of close contacts in neuronal cells in vivo.
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http://dx.doi.org/10.1242/jcs.253443DOI Listing
June 2021

Effect of exercise intervention on primary insomnia: a meta-analysis.

J Sports Med Phys Fitness 2021 Jun;61(6):857-866

Institute of Sport Science, Sichuan University, Chengdu, China -

Introduction: Exercise can have a positive effect on body functioning and immunity. This study systematically evaluated the effect of exercise intervention on patients diagnosed with primary insomnia. Based on the meta-analysis, we developed exercise recommendations to help improving sleep quality in these patients.

Evidence Acquisition: Using a combination of manual and electronic retrieval strategies, we examined the CNKI, VIP, Wanfang, Web of Science, SpringerLink, EBSCO, PubMed, Cochrane Library, and Embase databases; and identified randomized controlled trials that addressed the effects of exercise intervention on primary insomnia and were published before October 2019. Risk of bias was assessed based on the Cochrane Handbook 5.1.0, and meta-analysis was conducted using STATA 13.0 (StataCorp LLC; College Station, TX, USA).

Evidence Synthesis: Data were included for 1269 patients who received exercise interventions and 1203 patients who received drug therapy or no intervention (controls) in 23 trials of high quality. Meta-analysis showed that exercise intervention had a significant effect on the treatment of primary insomnia (SMD: -1.64, 95% CI: -2.08 to -1.19, P<0.001). Subgroup analysis showed significant effect sizes for older patients (>60 years) (SMD: -1.69, 95% CI: -2.40 to -0.97, P<0.001), aerobic exercise (SMD: -2.21, 95% CI: -2.89 to -1.53, P<0.001), interventions lasting 8-12 weeks (SMD: -2.58, 95% CI: -3.61 to -1.54, P<0.001), interventions lasting ≤60 min (SMD: -2.29, 95% CI: -3.66 to -0.92, P=0.001), Asian patients (SMD: -1.86, 95% CI: -2.42 to -1.31, P<0.001), and interventions ≤4 times/week (SMD: -1.70, 95% CI: -2.29 to -1.11, P<0.001). Both bias and sensitivity analyses suggested that our meta-analysis gave robust results.

Conclusions: Our results indicate that exercise intervention has a significant positive influence on primary insomnia, especially for older patients. We recommend that an exercise regimen of 60 min 4-5 times a week for 8-12 weeks can improve sleep quality and treat the symptoms of primary insomnia. Future studies should focus on verifying and extending our results by increasing sample size and improving the quality of studies included.
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http://dx.doi.org/10.23736/S0022-4707.21.11443-4DOI Listing
June 2021

Alterations in the RTK/Ras/PI3K/AKT pathway serve as potential biomarkers for immunotherapy outcome of diffuse gliomas.

Aging (Albany NY) 2021 Jun 8;13. Epub 2021 Jun 8.

Department of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, China.

Background: Diffuse gliomas are the most common malignant brain tumors, and immune checkpoint inhibitors have limited therapeutic effects against this cancer. Three oncogenic pathways are altered in diffuse gliomas: the RTK/Ras/PI3K/AKT signaling, TP53, and RB pathways. Although these pathways may affect the tumor immune microenvironment, their association with immunotherapy biomarkers remains unclear.

Methods: We used copy number variation and mutation data to stratify patients with specific oncogenic signaling alterations, and evaluated their correlation with predictive immunotherapy biomarkers, including tumor mutation burden (TMB), immune cytolytic activity (CYT), tumor purity, and tumor-infiltrating CD8 T cells. Immune checkpoint expression and interferon-γ signaling activity were also compared in these samples.

Results: We identified differentially expressed genes in three distinct oncogenic pathways. Gene ontology analysis of these genes revealed the involvement of RTK/Ras/PI3K/AKT-associated genes in immune and inflammatory responses. Moreover, significantly elevated TMB, CYT, and numbers of CD8 T cells and decreased tumor purity were correlated with altered RTK/Ras/PI3K/AKT signaling. Single cell sequencing also confirmed that this tumor subgroup had increased immune checkpoint expression and interferon-γ signaling activity. Immune phenotyping based on the presence of CD274 and TMB or CD274 and CD8 T cells indicated that tumors with altered RTK/Ras/PI3K/AKT pathways represent a beneficial subtype and are associated with improved survival.

Conclusion: Altered RTK/Ras/PI3K/AKT signaling and immunotherapy biomarkers are strongly correlated in gliomas. Gliomas with altered expression of RTK/Ras/PI3K/AKT pathway components may be sensitive to immunotherapy. A combination of small-molecule kinase inhibitors and immunotherapy is proposed for this subgroup of tumors.
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http://dx.doi.org/10.18632/aging.203102DOI Listing
June 2021

Impact of Using Median vs. Mean in Calculating ERBB2 FISH Results in Breast Cancer.

Cancer Med J 2021 Dec 15;4(3):87-96. Epub 2021 Apr 15.

Department of Pathology, The University of Texas Medical Branch, Galveston, Texas, USA.

Introduction: Erb-b2 receptor tyrosine kinase 2 (ERBB2) testing is used to measure the status of ERBB2 gene expression and DNA amplification. Test results have been reported with a discrepancy as high as 20%. The purpose of this study was to improve ERBB2 fluorescence in situ hybridization (FISH) sensitivity by evaluating results generated by median as well as mean calculations.

Methods: We retrospectively identified breast cancer cases at our institution in which ERBB2 FISH testing was performed in-house from June 2018 to May 2020. FISH results were classified using the 2018 American Society of Clinical Oncology/College of American Pathologists guidelines: groups 1 and 5 are FISH positive and negative, respectively, and groups 2-4 are equivocal requiring additional work-up. FISH counting sheets were collected and regrouped by median ERBB2 copy number counts and median ERBB2/CEP17 ratio and compared with the mean ERBB2 and mean ERBB2/CEP17 ratio. Intra-tumor genetic heterogeneity and CEP17 copy number gain (CEP17 ≥3) were assessed to see if they affect the discrepancy between median and mean groups.

Results: Seventy-two breast cancer cases were collected and evaluated. Eleven cases (11 of 72 [15%]) had discrepant grouping by mean and median calculations. A significant number of discrepancies were found for CEP17 copy number gain (p = 0.027) but not for ERBB2 (p = 0.411), the ERBB2/CEP17 ratio (p = 0.445), FISH results (p = 0.194), or genetic heterogeneity (p = 0.465). Among the four cases regrouped to median group 1, 2 were from mean group 3 and underwent anti-ERBB2 targeted therapy and 2 were from mean groups 4 and 5 may have benefitted from targeted therapy with more than 30% amplified cells. The median may be better to reflect the monosomy subclone within tumor tissues for the case 387 moved from mean group 5 to median group 2. The 6 cases moved from mean group 5 to median group 4 with CEP17 copy number gain may have had a poor prognosis based on other study result.

Conclusion: Including the median calculation may increase ERBB2 sensitivity and identification of CEP17 copy number gain. Further clinical studies are necessary to examine the outcome of including median in calculating ERBB2/CEP17 values.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171267PMC
December 2021

Mine Vegetation Identification via Ecological Monitoring and Deep Belief Network.

Plants (Basel) 2021 May 30;10(6). Epub 2021 May 30.

School of Environmental Studies, China University of Geosciences, Wuhan 430074, China.

Based on the characteristics of remote sensing images of mine vegetation, this research studied the application of deep belief network model in mine vegetation identification. Through vegetation identification and classification, the ecological environment index of mining area was determined according to the analysis of vegetation and coverage. Deep learning algorithm is adopted to improve the depth study, the vegetation coverage in the analysis was studied. Parameters and parameter values were selected for identification by establishing the optimal experimental design. The experimental results were compared with remote sensing images to determine the accuracy of deep learning identification and the effectiveness of the algorithm. When the sample size is 2,000,000 pixels, through repeated tests and classification effect comparison, the optimal parameter setting suitable for mine vegetation identification is obtained. Parameter setting: the number of network layers is 3 layers; the number of hidden layer neurons is 60. The learning rate is 0.01 and the number of iterations is 2. The average recognition rate of vegetation coverage was 95.95%, outperforming some other models, and the accuracy rate of kappa coefficient was 0.95, which can accurately reflect the vegetation coverage. The clearer the satellite image is, the more accurate the recognition result is, and the accuracy is closer to 100%. The identification of vegetation coverage has important guiding significance for determining the area and area of ecological restoration.
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http://dx.doi.org/10.3390/plants10061099DOI Listing
May 2021

Inflammatory myofibroblastic tumors arising from pancreas head and peri-splenic area mimicking a malignancy.

Ann Hepatobiliary Pancreat Surg 2021 May;25(2):287-292

Department of Pathology, Dong-A University Hospital, Dong-A University College of Medicine, Busan, Korea.

Inflammatory myofibroblastic tumors (IMTs) are a rare chronic inflammatory disease with unclear pathogenesis and pathological features that are not those of a malignant tumor. It is difficult to differentially diagnose them without surgical excision because of their unpredictable clinical behavior, which ranges from benign to locally invasive aggressiveness. We report two cases of IMTs that were diagnosed after surgery. In one case, the IMT originated in peri-splenic area in a 63-year-old female patient. The other case involved a 48-year-old female patient who suffered from an IMT of the head of the pancreas. Both of these cases did not require further treatment based on histological findings, and there has been no evidence of recurrence or metastasis so far. These cases show that the primary choice for the exact diagnosis and proper treatment of IMTs is complete surgical resection.
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http://dx.doi.org/10.14701/ahbps.2021.25.2.287DOI Listing
May 2021

The effect of dipeptidyl peptidase IV on disease-associated microglia phenotypic transformation in epilepsy.

J Neuroinflammation 2021 May 11;18(1):112. Epub 2021 May 11.

Department of Pathophysiology, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan, 430071, China.

Background: Accumulating evidence suggests that disease-associated microglia (DAM), a recently discovered subset of microglia, plays a protective role in neurological diseases. Targeting DAM phenotypic transformation may provide new therapeutic options. However, the relationship between DAM and epilepsy remains unknown.

Methods: Analysis of public RNA-sequencing data revealed predisposing factors (such as dipeptidyl peptidase IV; DPP4) for epilepsy related to DAM conversion. Anti-epileptic effect was assessed by electroencephalogram recordings and immunohistochemistry in a kainic acid (KA)-induced mouse model of epilepsy. The phenotype, morphology and function of microglia were assessed by qPCR, western blotting and microscopic imaging.

Results: Our results demonstrated that DPP4 participated in DAM conversion and epilepsy. The treatment of sitagliptin (a DPP4 inhibitor) attenuated KA-induced epilepsy and promoted the expression of DAM markers (Itgax and Axl) in both mouse epilepsy model in vivo and microglial inflammatory model in vitro. With sitagliptin treatment, microglial cells did not display an inflammatory activation state (enlarged cell bodies). Furthermore, these microglia exhibited complicated intersections, longer processes and wider coverage of parenchyma. In addition, sitagliptin reduced the activation of NF-κB signaling pathway and inhibited the expression of iNOS, IL-1β, IL-6 and the proinflammatory DAM subset gene CD44.

Conclusion: The present results highlight that the DPP4 inhibitor sitagliptin can attenuate epilepsy and promote DAM phenotypic transformation. These DAM exhibit unique morphological features, greater migration ability and better surveillance capability. The possible underlying mechanism is that sitagliptin can reduce the activation of NF-κB signaling pathway and suppress the inflammatory response mediated by microglia. Thus, we propose DPP4 may act as an attractive direction for DAM research and a potential therapeutic target for epilepsy.
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http://dx.doi.org/10.1186/s12974-021-02133-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114532PMC
May 2021

The Upregulation of a Novel Long Noncoding RNA AK097647 Promotes Enterovirus 71 Replication and Decreases IFN-λ1 Secretion.

Intervirology 2021 May 5:1-9. Epub 2021 May 5.

Shenzhen Institute of Wuhan University, Shenzhen, China.

Background: Enterovirus 71 (EV71) infects millions of children every year in China and has become a challenge to public health. However, there is no effective treatment for EV71 infection. Long noncoding RNAs (lncRNAs) have been found to play various roles in virus replication and infection.

Objective: We aimed to explore the role of a novel long noncoding RNA AK097647 (lncRNA-AK097647) during EV71 infection.

Methods: To assess the role of lncRNA-AK097647 during EV71 infection, siRNAs were used to silence lncRNA-K097647 expression. RT-qPCR assay and Western blotting were applied to measure the mRNA and protein levels of EV71 VP1 and the phosphorylation of NF-κB. ELISA was used to detect the level of IFN-λ1 expression.

Results: The novel lncRNA-AK097647 was upregulated in human rhabdomyosarcoma cells and the blood of hand, foot, and mouth disease patients infected with EV71, as demonstrated by RT-qPCR. Interestingly, RNAi-mediated knockdown of lncRNA-AK097647 dramatically increased the level of IFN-λ1 expression, resulting in the suppression of EV71 replication. In contrast, overexpression of lncRNA-AK097647 decreased the level of IFN-λ1 expression and resulted in increased EV71 replication. In addition, we found that lncRNA-AK097647 could inhibit the phosphorylation of NF-κB.

Conclusion: These results suggest a novel mechanism by which EV71 evades the IFN-mediated host antiviral response by increasing lncRNA-AK097647 expression.
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http://dx.doi.org/10.1159/000515903DOI Listing
May 2021

Simultaneous Use of Bioabsorbable and Nonbioabsorbable Packing Materials During Closed Reduction of a Nasal Bone Fractures.

J Craniofac Surg 2021 Apr 29. Epub 2021 Apr 29.

Department of Plastic and Reconstructive Surgery, Konkuk University School of Medicine, Konkuk University Chungju Hospital, Chungju, South Korea.

Abstract: The nasal packing material after closed reduction of nasal bone fracture may be bioabsorbable or nonbioabsorbable. To investigate the usefulness of the packing method using bioabsorbable and nonbioabsorbable materials simultaneously, a chart review was retrospectively performed involving 255 patients. Group A (n = 119), Nasopore (bioabsorbable) was cut appropriately and packed, and subsequently, supportive packing with Merocel (nonbioabsorbable) was performed. Group B (n = 78), nasal packing was performed with Vaseline roll gauze. Group C (n = 49), packed only Merocel. Pain and rhinorrhea were significantly lower in Merocel + Nasopore group (P < 0.05). It is thought that rhinorrhea is reduced by less mucosal irritation and maximizing the discharge absorption capacity by using Merocel and Nasopore simultaneously. By packing Nasopore first, pain is thought to be reduced by reducing the contact surface between Merocel and the injured mucosa and preventing a direct force to the fracture site. Therefore, our packing method, which uses Merocel and Nasopore simultaneously, can be recommended because it can provide sufficient support for the reduced fracture site, cause less pain, and maximize discharge absorption.
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http://dx.doi.org/10.1097/SCS.0000000000007705DOI Listing
April 2021

A New Method Based on Time-Varying Filtering Intrinsic Time-Scale Decomposition and General Refined Composite Multiscale Sample Entropy for Rolling-Bearing Feature Extraction.

Entropy (Basel) 2021 Apr 11;23(4). Epub 2021 Apr 11.

Undergraduate College, Songsim Global Campus, The Catholic University of Korea, Bucheon-si 14662, Korea.

The early fault diagnosis of rolling bearings has always been a difficult problem due to the interference of strong noise. This paper proposes a new method of early fault diagnosis for rolling bearings with entropy participation. First, a new signal decomposition method is proposed in this paper: intrinsic time-scale decomposition based on time-varying filtering. It is introduced into the framework of complete ensemble intrinsic time-scale decomposition with adaptive noise (CEITDAN). Compared with traditional intrinsic time-scale decomposition, intrinsic time-scale decomposition based on time-varying filtering can improve frequency-separation performance. It has strong robustness in the presence of noise interference. However, decomposition parameters (the bandwidth threshold and B-spline order) have significant impacts on the decomposition results of this method, and they need to be artificially set. Aiming to address this problem, this paper proposes rolling-bearing fault diagnosis optimization based on an improved coyote optimization algorithm (COA). First, the minimal generalized refined composite multiscale sample entropy parameter was used as the objective function. Through the improved COA algorithm, optimal intrinsic time-scale decomposition parameters based on time-varying filtering that match the input signal are obtained. By analyzing generalized refined composite multiscale sample entropy (GRCMSE), whether the mode component is dominated by the fault signal is determined. The signal is reconstructed and decomposed again. Finally, the mode component with the highest energy in the central frequency band is selected for envelope spectrum variation for fault diagnosis. Lastly, simulated and experimental signals were used to verify the effectiveness of the proposed method.
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http://dx.doi.org/10.3390/e23040451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069719PMC
April 2021

Effects of network structures on the tensile toughness of copper-catalyzed azide-alkyne cycloaddition (CuAAC)-based photopolymers.

Macromolecules 2021 Jan 4;54(2):747-756. Epub 2021 Jan 4.

Department of Chemical and Biological Engineering, University of Colorado Boulder, 596 UCB, Boulder, CO, United States.

In the present study, the photo-initiated copper-catalyzed azide-alkyne cycloaddition (CuAAC) polymerization was utilized to form structurally diverse glassy polymer networks. Systematic alterations in the monomer backbone rigidity (e.g., cyclic or aliphatic groups with a different length of backbone) and the reactive functional group density (e.g., tetra-, tri-, di-, and mono-functional azide and alkyne monomers) were used to provide readily tailorable network structures with crosslink densities (estimated from the rubbery modulus) varying by a factor of over 20. All eight of the resultant networks exhibited glass transition temperatures (T) between 50 and 80 °C with tensile toughness ranging from 28 to 61 MJ m. A nearly linear dependence of yield stress and elongation at break (broadly defined as strength and ductility, respectively) on the T and rubbery modulus was established in these triazole networks. When a flexible di-alkyne monomer (5 carbon spacing between alkynes) was incorporated in a network composed of a tri-alkyne and di-azide monomer, the elongation at break was improved from 166 to 300 %, while the yield stress was reduced from 36 to 23 MPa. Additionally, the polymer ductility was also varied by incorporating mono-functional azides as chain ends in the network - replacing a sterically hindered stiff mono-azide with a more flexible mono-azide increased the elongation at break from 24 to 185 % and the tensile toughness from 6 to 28 MJ m.
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http://dx.doi.org/10.1021/acs.macromol.0c02455DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057713PMC
January 2021

Increasing Age Associated with Higher Dipeptidyl Peptidase-4 Inhibition Rate Is a Predictive Factor for Efficacy of Dipeptidyl Peptidase-4 Inhibitors.

Diabetes Metab J 2021 Apr 19. Epub 2021 Apr 19.

Department of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.

Background: It is not known which type 2 diabetes mellitus (T2DM) patients would most benefit from dipeptidyl peptidase-4 (DPP-4) inhibitor treatment. We aimed to investigate the predictors of response to DPP-4 inhibitors considering degree of DPP-4 inhibition.

Methods: This study is a post hoc analysis of a 24-week, randomized, double-blind, phase III trial that compared the efficacy and safety of a DPP-4 inhibitor (gemigliptin vs. sitagliptin) in patients with T2DM. Subjects were classified into tertiles of T1 <65.26%, T2=65.26%-76.35%, and T3 ≥76.35% by DPP-4 inhibition. We analyzed the change from baseline in glycosylated hemoglobin (HbA1c) according to DPP-4 inhibition with multiple linear regression adjusting for age, ethnicity, body mass index, baseline HbA1c, and DPP-4 activity at baseline.

Results: The mean age was greater in the high tertile group compared with the low tertile group (T1: 49.8±8.3 vs. T2: 53.1±10.5 vs. T3: 55.3±9.5, P<0.001) of DPP-4 inhibition. Although HbA1c at baseline was not different among tertiles of DPP-4 inhibition (P=0.398), HbA1c after 24-week treatment was lower in the higher tertile compares to the lower tertile (T1: 7.30%±0.88% vs. T2: 7.12%±0.78% vs. T3: 7.00%±0.78%, P=0.021). In multiple regression analysis, DPP-4 enzyme inhibition rate was not a significant determent for HbA1c reduction due to age. In subgroup analysis by tertile of DPP-4 inhibition, age was the only significant predictor and only in the highest tertile (R2=0.281, B=-0.014, P=0.024).

Conclusion: This study showed that HbA1c reduction by DPP-4 inhibitor was associated with increasing age, and this association was linked with higher DPP-4 inhibition.
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http://dx.doi.org/10.4093/dmj.2020.0253DOI Listing
April 2021

Effect of diabetes mellitus on long-term outcomes of surgical revascularization in patients with ischemic heart failure: a propensity score-matching study.

Chin Med J (Engl) 2021 04 1;134(10):1146-1151. Epub 2021 Apr 1.

Department of Cardiovascular Surgery, Institute of Cardiac Surgery, PLA General Hospital, Institute of Cardiac Surgery, Beijing 100853, China.

Background: Diabetes mellitus (DM) is an important risk factor in the long-term outcomes of surgical revascularization. However, few studies have focused on patients with ischemic heart failure (IHF) and DM, and the results are controversial. This study aimed to evaluate the effect of DM on the long-term outcomes of IHF patients undergoing coronary artery bypass grafting (CABG).

Methods: In this propensity-matched study, data of IHF patients who underwent CABG in our hospital from January 2007 to December 2017 were analyzed. With a mean 73-month follow-up time, the patients were divided into two groups according to whether they had DM. The primary endpoint was all-cause death, and the secondary endpoint was a composite of all-cause death, stroke, recurrent myocardial infarction, and revascularization.

Results: There was no significant difference in all-cause mortality between the two groups (5.8% vs. 4.1%, P = 0.216). The incidence of main adverse cardiovascular and cerebrovascular events (MACCE) in the secondary endpoint was significantly higher in the DM group than that in the non-DM group (10.4% vs. 8.1%, P = 0.023).

Conclusions: DM can negatively affect the long-term outcomes of IHF patients undergoing CABG by significantly increasing the overall incidence of MACCE, though the long-term survival does not show a significant difference between the DM and non-DM patients.
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http://dx.doi.org/10.1097/CM9.0000000000001421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143767PMC
April 2021

The prevalence and risk factors of musculoskeletal disorders in the hands of fishermen working as oyster shuckers.

Int J Occup Med Environ Health 2021 Mar 30. Epub 2021 Mar 30.

Chosun University Hospital, Gwang-ju, Republic of Korea (Department of Occupational and Environmental Medicine).

Objectives: This study aimed to investigate the prevalence of musculoskeletal disorders in the hands and wrists of fishermen working as oyster shuckers, and to identify the risk factors associated with oyster shucking.

Material And Methods: A total of 590 fishery workers including those working as oyster shuckers in 2015-2017 were included in the study analysis. Hand osteoarthritis (OA), carpal tunnel syndrome (CTS), and ulnar impaction syndrome were diagnosed using X-ray, magnetic resonance imaging, and physical examinations according to each diagnostic criterion. The Rapid Entire Body Assessment was used as a measurement tool for analyzing the occupation-related burden factors.

Results: The prevalence of hand OA, CTS, and ulnar impaction syndrome was higher in oyster shuckers than in aquaculture/offshore fishery workers. The prevalence of upper limb disease was higher in oyster shuckers than in other fishery workers, even in the prevalence of exposure to occupational burden factors. In oyster shuckers, the incidence of hand and wrist OA was 84%, that of CTS was 72%, and that of ulnar impaction syndrome was 41%. In addition, oyster shuckers were more likely to be exposed to occupational burden factors than were other types of fishermen.

Conclusions: The prevalence of hand OA, CTS, and ulnar impaction syndrome was higher in oyster shuckers. Therefore, proper stretching or exercises for rest and musculoskeletal relaxation, along with health-care education and promotion, will be needed for the fishermen's repeated posture and impact work.
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http://dx.doi.org/10.13075/ijomeh.1896.01752DOI Listing
March 2021

One-Step Synthesis of Water-Soluble CdS Quantum Dots for Silver-Ion Detection.

ACS Omega 2021 Mar 4;6(10):7139-7146. Epub 2021 Mar 4.

College of Forestry, Northeast Forestry University, Harbin 150040, China.

To realize fast synthesis of cadmium sulfide (CdS) quantum dots with a low-toxic material, a one-step synthesis method is investigated and conducted. Potato extract is used as a stabilizer and modifier, by which aqueous CdS quantum dots can be prepared at a lower temperature with a shorter time. Through systematic characterization and analysis, a green and fast synthesis mechanism is demonstrated in detail. And the nanoscale CdS quantum dots are uniform in size and dispersity. With low cost and high sensitivity, the prepared CdS quantum dots show promising application in silver-ion detection. This method shows great significance for an environmentally friendly and facile synthesis of CdS quantum dots.
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http://dx.doi.org/10.1021/acsomega.1c00162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970548PMC
March 2021

Improving the Efficiency of Quantum Dot Sensitized Solar Cells beyond 15% via Secondary Deposition.

J Am Chem Soc 2021 Mar 18;143(12):4790-4800. Epub 2021 Mar 18.

Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, People's Republic of China.

Low loading is one of the bottlenecks limiting the performance of quantum dot sensitized solar cells (QDSCs). Although previous QD secondary deposition relying on electrostatic interaction can improve QD loading, due to the introduction of new recombination centers, it is not capable of enhancing the photovoltage and fill factor. Herein, without the introduction of new recombination centers, a convenient QD secondary deposition approach is developed by creating new adsorption sites via the formation of a metal oxyhydroxide layer around QD presensitized photoanodes. MgCl solution treated Zn-Cu-In-S-Se (ZCISSe) QD sensitized TiO film electrodes have been chosen as a model device to investigate this secondary deposition approach. The experimental results demonstrate that additional 38% of the QDs are immobilized on the photoanode as a single layer. Due to the increased QD loading and concomitant enhanced light-harvesting capacity and reduced charge recombination, not only photocurrent but also photovoltage and fill factor have been remarkably enhanced. The average PCE of resulted ZCISSe QDSCs is boosted to 15.31% ( = 26.52 mA cm, = 0.802 V, FF = 0.720), from the original 13.54% ( = 24.23 mA cm, = 0.789 V, FF = 0.708). Furthermore, a new certified PCE record of 15.20% has been obtained for liquid-junction QDSCs.
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http://dx.doi.org/10.1021/jacs.1c01214DOI Listing
March 2021

Is immunonutrition superior to standard enteral nutrition in reducing postoperative complications in patients undergoing esophagectomy? A meta-analysis of randomized controlled trials.

J BUON 2021 Jan-Feb;26(1):204-210

UT Health San Antonio Department of Radiation Oncology, San Antonio, Texas, USA.

Purpose: Perioperative enteral nutrition supports are recommended in esophagus cancer patients. Immunonutrition contains immuno-enhancing nutrients in addition to standard formula. These new nutrients are thought to be efficacious in reducing inflammatory response and improving postoperative immune response and they have been proved to be better than standard enteral nutrition in reducing postoperative complications in gastric cancer. However, if it would lead to a better clinical outcome in patients undergoing esophagectomy remains controversial.

Methods: A systematic literature search was performed in the online database of PubMed, Medline, EMBASE and Cochrane Library. The relevant studies were screened out of the results by reading titles and abstracts. Then, we read the full-texts to finally confirm the studies included in this meta-analysis.

Results: Six randomized controlled trials having enrolled 638 patients were included in the final analysis. The pooled analysis didn't show statistically significant difference between immunonutrition group and standard nutrition group in reducing postoperative complications.

Conclusions: The postoperative complications are comparable between immunonutrition and the standard enteral nutrition in patients undergoing esophagectomy, but its value in severe malnutrition patients is undetermined, whereas the high tolerance and other advantages brought by the immunonutrition should not be overlooked and need to be further proved.
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March 2021

Maternal Lead Exposure Impairs Offspring Learning and Memory via Decreased GLUT4 Membrane Translocation.

Front Cell Dev Biol 2021 25;9:648261. Epub 2021 Feb 25.

Department of Occupational and Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an, China.

Lead (Pb) can cause a significant neurotoxicity in both adults and children, leading to the impairment to brain function. Pb exposure plays a key role in the impairment of learning and memory through synaptic neurotoxicity, resulting in the cognitive function. Researches have demonstrated that Pb exposure plays an important role in the etiology and pathogenesis of neurodegenerative diseases, such as Alzheimer's disease. However, the underlying mechanisms remain unclear. In the current study, a gestational Pb exposure (GLE) rat model was established to investigate the underlying mechanisms of Pb-induced cognitive impairment. We demonstrated that low-level gestational Pb exposure impaired spatial learning and memory as well as hippocampal synaptic plasticity at postnatal day 30 (PND 30) when the blood concentration of Pb had already recovered to normal levels. Pb exposure induced a decrease in hippocampal glucose metabolism by reducing glucose transporter 4 (GLUT4) levels in the cell membrane through the phosphatidylinositol 3 kinase-protein kinase B (PI3K-Akt) pathway. and GLUT4 over-expression increased the membrane translocation of GLUT4 and glucose uptake, and reversed the Pb-induced impairment to synaptic plasticity and cognition. These findings indicate that Pb exposure impairs synaptic plasticity by reducing the level of GLUT4 in the cell membrane as well as glucose uptake via the PI3K-Akt signaling pathway, demonstrating a novel mechanism for Pb exposure-induced neurotoxicity.
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http://dx.doi.org/10.3389/fcell.2021.648261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947239PMC
February 2021

Detection of aflatoxins B1 in  peanut oil using ATR-FTIR spectroscopy combined with partial least squares discriminant analysis and support vector machine models.

J Food Prot 2021 Mar 12. Epub 2021 Mar 12.

Jinan University Huangpu street CHINA Guangzhou 510000.

This study establishes a rapid and accurate method to identify aflatoxin contamination in peanut oil. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, combined with either partial least squares discriminant analysis (PLS-DA) or a support vector machine (SVM) algorithm, were used to construct discriminative models for distinguishing between uncontaminated and aflatoxin-contaminated peanut oil. Peanut oil samples containing various concentrations of aflatoxin B1 (AFB1) were subjected to spectroscopic examination using an ATR-FTIR spectrometer. Preprocessed spectral data were input to PLS-DA and SVM algorithms to construct discriminative models for aflatoxin contamination in peanut oil. SVM penalty and kernel function parameters were optimized using grid search, a genetic algorithm, and particle swarm optimization. Results demonstrated that with the PLS-DA model established using spectral data, with an accuracy of 94.64%, exhibited better discriminative capacities than models established based on preprocessed data. The SVM model established after data normalization and grid search optimization with a penalty parameter of 16 and kernel function parameter of 0.0359 displayed the best discriminative capacity, with 98.2143% accuracy. The discriminative models for aflatoxin contamination in peanut oil established by combining ATR-FTIR spectral data and nonlinear SVM algorithm were superior to the linear PLS-DA models.
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http://dx.doi.org/10.4315/JFP-20-447DOI Listing
March 2021

Detection of aflatoxins B1 in  peanut oil using ATR-FTIR spectroscopy combined with partial least squares discriminant analysis and support vector machine models.

J Food Prot 2021 Mar 12. Epub 2021 Mar 12.

Jinan University Huangpu street CHINA Guangzhou 510000.

This study establishes a rapid and accurate method to identify aflatoxin contamination in peanut oil. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, combined with either partial least squares discriminant analysis (PLS-DA) or a support vector machine (SVM) algorithm, were used to construct discriminative models for distinguishing between uncontaminated and aflatoxin-contaminated peanut oil. Peanut oil samples containing various concentrations of aflatoxin B1 (AFB1) were subjected to spectroscopic examination using an ATR-FTIR spectrometer. Preprocessed spectral data were input to PLS-DA and SVM algorithms to construct discriminative models for aflatoxin contamination in peanut oil. SVM penalty and kernel function parameters were optimized using grid search, a genetic algorithm, and particle swarm optimization. Results demonstrated that with the PLS-DA model established using spectral data, with an accuracy of 94.64%, exhibited better discriminative capacities than models established based on preprocessed data. The SVM model established after data normalization and grid search optimization with a penalty parameter of 16 and kernel function parameter of 0.0359 displayed the best discriminative capacity, with 98.2143% accuracy. The discriminative models for aflatoxin contamination in peanut oil established by combining ATR-FTIR spectral data and nonlinear SVM algorithm were superior to the linear PLS-DA models.
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http://dx.doi.org/10.4315/JFP-20-447DOI Listing
March 2021

[Acute cerebral ischemia-induced down-regulation of Sirt3 protein expression contributes to neuronal injury via damaging mitochondrial function].

Sheng Li Xue Bao 2021 Feb;73(1):17-25

Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.

This study was aimed to determine the effect of acute cerebral ischemia on the protein expression level of silent mating type information regulator 2 homolog 3 (Sirt3) in the neurons and clarify the pathological role of Sirt3 in acute cerebral ischemia. The mice with middle cerebral artery occlusion (MCAO) and primary cultured rat hippocampal neurons with oxygen glucose deprivation (OGD) were used as acute cerebral ischemia models in vivo and in vitro, respectively. Sirt3 overexpression was induced in rat hippocampal neurons by lentivirus transfection. Western blot was utilized to measure the changes in Sirt3 protein expression level. CCK8 assay was used to detect cell viability. Immunofluorescent staining was used to detect mitochondrial function. Transmission electron microscope was used to detect mitochondrial autophagy. The results showed that, compared with the normoxia group, hippocampal neurons from OGD1 h/reoxygenation 2 h (R2 h) and OGD1 h/R12 h groups exhibited down-regulated Sirt3 protein expression levels. Compared with contralateral normal brain tissue, the ipsilateral penumbra region from MCAO1 h/reperfusion 24 h (R24 h) and MCAO1 h/R72 h groups exhibited down-regulated Sirt3 protein expression levels, while there was no significant difference between the Sirt3 protein levels on both sides of sham group. OGD1 h/R12 h treatment damaged mitochondrial function, activated mitochondrial autophagy and reduced cell viability in hippocampal neurons, whereas Sirt3 over-expression attenuated the above damage effects of OGD1 h/R12 h treatment. These results suggest that acute cerebral ischemia results in a decrease in Sirt3 protein level. Sirt3 overexpression can alleviate acute cerebral ischemia-induced neural injuries by improving the mitochondrial function. The current study sheds light on a novel strategy against neural injuries caused by acute cerebral ischemia.
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February 2021

[Changes in microglia number and Iba1 expression level in the prefrontal cortex of type 1 diabetic mice].

Sheng Li Xue Bao 2021 Feb;73(1):10-16

Department of Neurobiology, School of Basic Medicine, Capital Medical University, Beijing 100069, China.

The aim of the present study was to observe the activation of microglia in the prefrontal cortex of type 1 diabetes mellitus (T1DM) mice, and the expression of the marker genes of the disease-associated microglia (DAM) associated with neurodegenerative diseases. Sixty healthy adult male C57BL/6J mice were randomly divided into two groups, normal control (CON) group and T1DM group. Streptozocin (STZ) was injected intraperitoneally to induce T1DM mice. The spatial learning and memory function of mice was detected by Morris water maze at the 8th week after the successful model establishment. The number and activation of microglia in the prefrontal cortex of mice were detected by immunofluorescence staining and Western blot. Changes in the mRNA level of several DAM molecular markers were detected by RT-FQ-PCR. The results showed that, compared with CON mice, the fasting blood glucose of T1DM mice increased significantly, while the body weight of T1DM mice decreased remarkably (P < 0.05). The escape latency of water maze in T1DM mice was longer than that in CON mice (P < 0.05). Compared with CON group, the Iba1 protein expression and the number of microglia in prefrontal cortex of T1DM group increased significantly (P < 0.05). In addition, the mRNA levels of several DAM markers in prefrontal cortex of T1DM group were increased significantly (P < 0.05). These results suggest that the microglia are activated and transformed to DAM type in the prefrontal cortex of T1DM mice.
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February 2021

In vivo reduction of hippocampal Caveolin-1 by RNA interference alters morphine addiction and neuroplasticity changes in male mice.

Neurosci Lett 2021 04 16;749:135742. Epub 2021 Feb 16.

Department of Anesthesiology, Beijing Tian Tan Hospital, Capital Medical University, #119 Nan Si Huan Xi Lu, Beijing, 100050, PR China. Electronic address:

Prescription opioids are powerful pain-controlling medications that have both benefits and potentially serious risks. Morphine is one of the preferred analgesics that are widely used to treat chronic pain. However, chronic morphine exposure has been found to cause both functional and structural changes in several brain regions, including the medial prefrontal cortex (mPFC), ventral tegmental area (VTA), and hippocampus (HPC), which lead to addictive behavior. Caveolin-1 (Cav-1), a scaffolding protein of membrane lipid rafts (MLRs), has been shown to organize GPCRs and multiple synaptic signaling proteins within the MLRs to regulate synaptic signaling and neuroplasticity. Previously, we showed that in vitro morphine treatment significantly elevates Cav-1 expression and causes neuroplasticity changes. In this study, we confirmed that chronic morphine exposure can significantly increase Cav-1 expression (P < 0.05) and microtubule-associated protein (MAP-2)-positive neuronal dendritic growth in the hippocampus. Moreover, the rewarding effect and dendritic growth in the HPC induced by chronic morphine exposure were significantly inhibited by hippocampal Cav-1 knockdown. Together, these data suggest that Cav-1 in the hippocampus plays an essential role in the neuroplasticity changes that underlie morphine addiction behaviors.
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http://dx.doi.org/10.1016/j.neulet.2021.135742DOI Listing
April 2021

LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 Axis.

Cancer Manag Res 2021 5;13:1067-1073. Epub 2021 Feb 5.

Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin Institute of Technology, Harbin, 150036, People's Republic of China.

Background: Long noncoding RNA (lncRNA) has been reported to play important roles in tumor initiation. However, how lncRNA ELFN1-AS1 affects retinoblastoma development remains unclear. Thus, we sought to elucidate its functions in retinoblastoma progression.

Methods: ELFN1-AS1 expression was measured in retinoblastoma tissues and normal tissues by qRT-PCR. CCK8, colony formation and Transwell assay were carried out to investigate the effects of ELFN1-AS1 knockdown on cell malignant behaviors. Bioinformatics analyses were performed to predict the relationship among ELFN1-AS1, miR-4270 and SBK1.

Results: ELFN1-AS1 was highly expressed in retinoblastoma tissues and cell lines. ELFN1-AS1 was positively correlated with retinoblastoma progression and prognosis. ELFN1-AS1 knockdown curtailed retinoblastoma proliferation, migration and invasion. ELFN1-AS1 was the competing endogenous RNA for miR-4270 and promoted SBK1expression.

Conclusion: Altogether, our findings demonstrated that ELFN1-AS1 promotes retinoblastoma progression through mediating miR-4270/SBK1 axis and might be a promising therapeutic target.
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http://dx.doi.org/10.2147/CMAR.S281536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872934PMC
February 2021

A nanograting-based flexible and stretchable waveguide for tactile sensing.

Nanoscale Res Lett 2021 Feb 5;16(1):23. Epub 2021 Feb 5.

School of Mechanical and Electronic Engineering, Wuhan University of Technology, Wuhan, 430070, China.

Based on the related characteristics of optical waveguide and flexible optical materials, a flexible and stretchable optical waveguide structure oriented to tactile perception is proposed. The sensing principle of optical waveguide is based on mechanical deformation caused by output light loss. It overcomes the shortcomings of traditional optical waveguide devices, which are unable to conform to irregular surface. The flexible and stretchable optical waveguide is fabricated with nanoreplica molding method, and it has been applied to the measurement of pressure and strain in the field of tactile sensing. The flexible and stretchable optical waveguide had a strain detection range of 0 to 12.5%, and the external force detection range is from 0 to 23 × 10 N.
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http://dx.doi.org/10.1186/s11671-021-03488-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865040PMC
February 2021

Influence of polyethylene-microplastic on environmental behaviors of metals in soil.

Environ Sci Pollut Res Int 2021 Jun 4;28(22):28329-28336. Epub 2021 Feb 4.

College of Forestry, Northeast Forestry University, Harbin, 150040, China.

Microplastics (MPs) in terrestrial ecosystems have attracted increasing attention all over the world. The adsorption-desorption behavior and bioavailability of metals in soil would affect its toxicity to organisms. However, the influences of MPs on adsorption-desorption behavior between metals and soil as well as bioavailability of metals in soils are scarcely investigated. Herein, different percentage (0, 0.1%, 1%, 10%) of polyethylene-microplastic (PE-MP) were thoroughly mixed into the soil to investigate the impacts of PE-MP on adsorption-desorption and bioavailability of metals (Zn, Pb) in the soil. A series of characterization were carried out to determine the change of PE-MP before and after adsorption to investigate the mechanisms. When MP100 (average size: 129 μm) content in soil increased to 10%, the adsorption capacities of soil with Pb and Zn were 3.73 and 4.56 mg/g, respectively, which were significantly (p < 0.05) lower than that of pure soil. When MP300 (average size: 293 μm) content in soil increased to 10%, the extraction fraction of Zn and Pb from soil by diethylenetriaminepentaacetic acid reached 12.35% and 23.96%, respectively, which were significantly (p < 0.05) higher than that of pure soil, indicating high concentration (10%) of MPs in soil would decrease the adsorption capability of soil to metals and increase the mobility of metals in terrestrial environment. However, when MPs content in soil was 0.1%, the extraction fraction of Zn and Pb showed no significant difference with that of pure soil, indicating that actual MPs in soil is unlikely to bring significant influence on metal bioavailability.
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http://dx.doi.org/10.1007/s11356-021-12718-yDOI Listing
June 2021

Optimal treatment for penile verrucous carcinoma: a systematic literature review.

BMC Urol 2021 Jan 29;21(1):13. Epub 2021 Jan 29.

Department of Urology, Konkuk University School of Medicine, Gukwon-daero 82, 27376, Chungju, Chungbuk, Republic of Korea.

Background: Verrucous carcinoma, a rare low-grade well-differentiated squamous cell carcinoma, is known for its favorable biological behavior and lack of metastatic potential. However, aggressive resection is problematic in terms of compromised function and aesthetics. Hence, more conservative treatments are needed.

Methods: To identify the up-to-date general biological behavior, diagnosis, and treatment trends, we searched PubMed using the keyword "penile verrucous carcinoma" without restrictions on publication date.

Results: Current treatments for penile verrucous carcinoma include wide surgical excision, seldom preventive lymphadenectomy, and conservative chemotherapy without surgery or local excision with safe margins. Despite the advent of partial penectomy to minimally impact function and aesthetics, affected patients experience psychosexual problems. Local excision can be used to save the penile shaft and glans penis without preventive lymphadenectomy or adjuvant therapy and can achieve good clinical prognosis with rare recurrence.

Conclusions: To preserve the functional and cosmetic aspects, we recommend local excision, especially for tumors measuring < 3 cm and classified as stage T1 according to the 2016 tumor node metastasis clinical and pathological classification for penile cancer.
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http://dx.doi.org/10.1186/s12894-020-00777-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844965PMC
January 2021

Genomic studies on natural and engineered aquatic denitrifying eco-systems: A research update.

Bioresour Technol 2021 Apr 20;326:124740. Epub 2021 Jan 20.

Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan; College of Technology and Engineering, National Taiwan Normal University, Taipei 10610, Taiwan; College of Engineering, Tunghai University, Taichung 40070, Taiwan. Electronic address:

Excess nitrogenous compounds in municipal or industrial wastewaters can stimulate growth of denitrifying bacteria, in return, to convert potentially hazardous nitrate to inorganic nitrogen gas. To explore the community structure, distributions and succession of functional strains, and their interactions with other microbial communities, contemporary studies were performed based on detailed genomic analysis. This mini-review updated contemporary genomic studies on denitrifying genes in natural and engineered aquatic systems, with the constructed wetlands being the demonstrative system for the latter. Prospects for the employment of genomic studies on denitrifying systems for process design, optimization and development of novel denitrifying processes were discussed.
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http://dx.doi.org/10.1016/j.biortech.2021.124740DOI Listing
April 2021

Analysis of multiple cytokines in aqueous humor of patients with idiopathic macular hole.

BMC Ophthalmol 2021 Jan 11;21(1):27. Epub 2021 Jan 11.

Department of Ophthalmology, The Fourth Hospital of Shenyang, 110016, Shenyang, Liaoning, People's Republic of China.

Background: Idiopathic macular holes are common ophthalmic manifestations with unknown pathogenesis. Thus far, there has been minimal research regarding the causes of idiopathic macular holes, especially with respect to the underlying immune mechanism. To provide clarity regarding the treatment and prognosis of idiopathic macular holes, specifically regarding the levels of cytokines in affected patients, this study examined and analyzed multiple cytokine levels in aqueous humor from patients with idiopathic macular holes.

Methods: This comparative cross-sectional study included 38 patients in two groups: a cataract control group (n = 17) and an idiopathic macular hole group (n = 21). The levels of 48 cytokines in aqueous humor were detected by multiplex analysis with antibody-coupled magnetic beads. The Kolmogorov-Smirnov test was used to check whether the data were normally distributed; Student's t-test and the Mann-Whitney U test were used to assess differences in cytokine levels between the two groups. Spearman correlation analysis was used to assess relationships among cytokine levels in the experimental group. Signaling pathways containing cytokines with significantly different expression in the experimental group were identified.

Results: There were significant differences in aqueous humor cytokine levels between patients with idiopathic macular holes and patients in the cataract control group. Notably, hepatocyte growth factor (p = 0.0001), GM-CSF (p = 0.0111), and IFN-γ (p = 0.0120) were significantly upregulated in the experimental group, while TNF-α (p = 0.0032), GRO-α (p < 0.0001), and MIF (p < 0.0001) were significantly downregulated in the experimental group. Furthermore, the GM-CSF level showed significant positive correlations with levels of IL-1 (r = 0.67904, p < 0.001), IL-4 (r = 0.76017, p < 0.001), and IFN-γ (r = 0.59922, p = 0.004097) in the experimental group. Moreover, the levels of nerve growth factor and hepatocyte growth factor showed a significant positive correlation (r = 0.64951, p = 0.001441) in the experimental group.

Conclusions: Patients with idiopathic macular holes showed significant variation in aqueous humor immune response after the onset of hole formation, including the recruitment of immune cells and regulation of cytokine expression. Our findings also suggest that it is not appropriate to use patients with macular holes as the control group in studies of aqueous humor cytokine levels in ophthalmic diseases.
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http://dx.doi.org/10.1186/s12886-020-01782-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802234PMC
January 2021

FISH and Chromosome Microarray Testing of Gastroesophageal Adenocarcinomas at a Single Institution.

Cancer Med J 2020 7;3(Suppl 1):39-46. Epub 2020 Nov 7.

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.

Overexpression/amplification of erb-b2 receptor tyrosine kinase 2 (ERBB2) is a major prognostic factor in gastroesophageal cancers; it is currently the only biomarker established for the selection of targeted therapy for patients with advanced gastroesophageal adenocarcinoma (GEA). Current standard procedure for determining ERBB2 status in such patients is immunohistochemistry (IHC), followed by in situ hybridization (ISH), when IHC result is equivocal. Insufficient knowledge regarding the utilities of chromosomal microarray (CMA) has hindered its use as an adjunct tool in ERBB2 analysis. Here, we performed CMA on 7 formalin-fixed paraffin-embedded (FFPE) GEA specimens previously tested by ERBB2 fluorescence in situ hybridization (FISH) and evaluated the concordance and performance of CMA. CMA identified 4 (57.1%) samples with amplification of ERBB2, compared to 3 (42.9%) by FISH. CMA also detected several additional DNA copy number variants in these samples, which may have prognostic and therapeutic indications. Further case studies and clinical trials may provide evidence for the utility of CMA-based genomic studies in the management of patients with suspected ERBB2-positive gastroesophageal adenocarcinoma.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790124PMC
November 2020