Publications by authors named "Somayeh Sharifynia"

14 Publications

  • Page 1 of 1

Fatal invasive pulmonary aspergillosis in an immunocompetent patient with COVID-19 due to : A case study.

Clin Case Rep 2021 Apr 16;9(4):2414-2418. Epub 2021 Mar 16.

Department of Hematology and Medical Oncology Shahid Beheshti University of Medical Sciences Tehran Iran.

Case reports of CAPA emerged. In most of the reports, the predominant species is . Uncommon species are less reported. Due to poor clinical outcome with , the increasing reports with this agent require attention.
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http://dx.doi.org/10.1002/ccr3.4051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077262PMC
April 2021

Diagnosis of allergic bronchopulmonary aspergillosis in patients with persistent allergic asthma using three different diagnostic algorithms.

Mycoses 2021 Mar 8;64(3):272-281. Epub 2020 Dec 8.

Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Background: Allergic bronchopulmonary aspergillosis (ABPA) has been reported in various degrees among patients with persistent allergic asthma (PAA). Currently, there is no gold standard approach for diagnosis of ABPA.

Objectives: In the current study, we aimed the evaluation of three different mainly used algorithms as Rosenberg & Patterson (A), ISHAM Working Group (B) and Greenberger (C) for diagnosis of ABPA in 200 patients with underlying PAA.

Methods: All patients were evaluated using Aspergillus skin prick test (SPTAf), Aspergillus-specific IgE (sIgEAf) and IgG (sIgGAf), total IgE (tIgE), pulmonary function tests, radiological findings and peripheral blood eosinophil count. The prevalence rate of ABPA in PAA patients was estimated by three diagnostic criteria. We used Latent Class Analysis for the evaluation of different diagnostic parameters in different applied ABPA diagnostic algorithms.

Results: Aspergillus sensitisation was observed in 30 (15.0%) patients. According to algorithms A, B and C, nine (4.5%), six (3.0%) and 11 (5.5%) of patients were diagnosed with ABPA, respectively. The sensitivity and specificity of criteria B and C were (55.6% and 99.5%) and (100.0% and 98.9%) respectively. sIgEAf and sIgGAf showed the high significant sensitivity. The performance of algorithm A, in terms of sensitivity and specificity, was somewhat better than algorithm B.

Conclusion: Our study demonstrated that the sensitivity of different diagnostic algorithms could change the prevalence rate of ABPA. We also found that all of three criteria resulted an adequate specificity for ABPA diagnosis. A consensus patterns combining elements of all three criteria may warrant a better diagnostic algorithm.
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http://dx.doi.org/10.1111/myc.13217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902363PMC
March 2021

Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens.

Rev Soc Bras Med Trop 2020 7;53:e20190214. Epub 2020 Feb 7.

Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran.

Introduction: The aim of this study was to evaluate some virulence factors in Candida albicans isolates from patients with onychomycosis and determine the correlation between these factors and the antifungal resistance profile.

Methods: Seventy species of C. albicans were confirmed using polymerase chain reaction amplification of the HWP1 gene. According to the Clinical & Laboratory Standards Institute guidelines, the susceptibility profile of four antifungal agents was investigated, and the production of aspartyl protease, phospholipase, haemolysin, and biofilm was determined. The correlation between these profiles was also investigated.

Results: The isolates indicated different levels of resistance and production of virulence factors. Significant correlations were observed between the minimum inhibitory concentration (MIC) of fluconazole/itraconazole and biofilm production, between phospholipase production and fluconazole/itraconazole MIC, and between fluconazole MIC and hemolytic activity in C. albicans isolates. The results also showed significant correlations between phospholipase activity and biofilm production.

Conclusions: Our findings will contribute to a better understanding of the pathogenesis of C. albicans and characterize the relationship between virulence factors and antifungal resistance, which may suggest new therapeutic strategies considering the possible involvement of the virulence mechanism in the effectiveness of treatment.
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http://dx.doi.org/10.1590/0037-8682-0214-2019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083380PMC
March 2020

Prevalence of specific immunoglobulin E and G against in patients with asthma.

Curr Med Mycol 2018 Dec;4(4):7-11

Invasive Fungi Research Centre, Mazandaran University of Medical Sciences, Sari, Iran.

Background And Purpose: as a ubiquitous fungus can be found in the respiratory tract of the asthmatic and healthy people. The inhalation of spores leads to an immune response in individuals with asthma and results in the aggravation of the clinical symptoms. The present study aimed to investigate the prevalence of specific immunoglobulin E and G IgE and IgG) against in asthmatic patients.

Materials And Methods: This study was conducted on 200 consecutive patients with moderate to severe asthma referring to Masih Daneshvari hospital Tehran, Iran, from January 2016 to February 2018. Skin prick test (SPT) was performed in all subjects with allergens. Moreover, all patients underwent specific IgE testing for using Hycor method. Enzyme immune assay was applied to measure total IgE and specific IgG.

Results: According to the results, the mean age of the patients was 45.8 years (age range: 18-78 years). The mean levels of total IgE and specific IgE in asthmatic patients were obtained as 316.3 (range: 6-1300 IU/ml) and 1.5 (range: 0.1-61.3 IU/ml), respectively. Out of 200 patients, 27 (13.5%), 65 (32.5%), 22 (11.0%), and 86 (43.0%) cases had positive SPT, total IgE of > 417 IU/ml, specific IgE, and IgG, respectively. The level of these variables in patients with severe asthma were 16 (16.5%), 36 (37.1%), 15 (15.5%), and 46 (47.4%), respectively.

Conclusion: As the findings indicated, reactivity to is a remarkable phenomenon in asthmatic patients. It is also emphasised that the climatic condition may affect the positive rate of hypersensitivity to .
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http://dx.doi.org/10.18502/cmm.4.4.380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386509PMC
December 2018

Genetic Diversity and Antifungal Susceptibility of Candida parapsilosis Sensu Stricto Isolated from Bloodstream Infections in Turkish Patients.

Mycopathologia 2018 Aug 3;183(4):701-708. Epub 2018 May 3.

Department of Microbiology and Immunology, Center of Excellence for Infection Biology and Antimicrobial Pharmacology, Tehran, Iran.

Candida parapsilosis sensu stricto is an emerging cause of hospital-acquired Candida infections, predominantly in southern Europe, South America, and Asia. We investigated the genetic diversity and antifungal susceptibility profile of 170 independent C. parapsilosis sensu stricto strains obtained from patients with candidemia who were treated at the Ege University Hospital in Izmir, Turkey, between 2006 and 2014. The identity of each strain was confirmed via PCR amplification and digestion of the secondary alcohol dehydrogenase-encoding gene. The 24-h geometric mean minimum inhibitory concentrations of the antifungal agents, in increasing order, were as follows: posaconazole, 0.10 µg/mL; voriconazole, 0.21 µg/mL; caspofungin, 0.38 µg/mL; amphotericin B, 0.61 µg/mL; anidulafungin, 0.68 µg/mL; and fluconazole, 2.95 µg/mL. Microsatellite genotyping of the isolates (using fluorescently labeled primers and a panel of four different short-nucleotide repeat fragments) identified 25, 17, 17, and 8 different allelic genotypes at the CP6, B5, CP4, and CP1 locus, respectively. Posaconazole, caspofungin, and amphotericin B showed the greatest in vitro activity of the tested systemic azole, echinocandin, and polyene agents, respectively, and the observed antifungal susceptibility of the isolates was shown to be independent of their isolation source. We obtained a combined discriminatory power of 0.99 with a total of 130 genotypes for 170 isolates tested. Finally, microsatellite profiling analysis confirmed the presence of identical genotype between separate isolates, supporting that effective surveillance and infection-prevention programs are essential to limit the impact of C. parapsilosis sensu stricto on hospitalized patients' health.
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http://dx.doi.org/10.1007/s11046-018-0261-xDOI Listing
August 2018

Morphological changes and induction of antifungal resistance in due to different CO2 levels.

Curr Med Mycol 2017 Sep;3(3):21-26

Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background And Purpose: Aspergillosis is one of the most common opportunistic fungal infections in immunocompromised and neutropenic patients. () is the most common causative agent of this infection. Due to variable CO concentrations that pathogens are exposed to during the infection process and to understand the role of CO, we examined the effects of various CO concentrations as one of the environmental factors on morphological changes and induction of antifungal resistance in .

Materials And Methods: strains were cultured and incubated under 1%, 3%, 5%, and 12% CO atmospheres, each time for one, two, and four weeks. The control culture was maintained for one week without CO atmosphere. Morphological changes were investigated and antifungal susceptibility test was performed according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI) M38-A2 document. The results of different CO atmospheres were compared with that of the control sample.

Results: We found that 1%, 3%, 5%, and 12% CO atmospheres were associated with morphological colony changes. Macroscopically, the colonies were shallow dark green, smooth, crisp to powdery with reduced growth; microscopic examination revealed the absence of conidiation. The induction of antifungal resistance in the susceptible strains to itraconazole, voriconazole, and amphotericin B increased after exposure to 12% CO atmosphere and four weeks of incubation. The MIC values for itraconazole, voriconazole, and amphotericin B were 16 g/ml, 1 g/ml, and 16 g/ml, respectively. These values for the control group were 0.125 g/ml, 0.125 g/ml, and 2 g/ml, respectively.

Conclusion: Exposure to different CO atmospheres induced morphological changes in , it seems to increase the MIC values, as well. In parallel, resistance to both itraconazole and voriconazole was also observed.
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http://dx.doi.org/10.29252/cmm.3.3.21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914923PMC
September 2017

Genetic diversity and antifungal susceptibility of Candida albicans isolated from Iranian patients.

Med Mycol 2019 Jan;57(1):127-131

Invasive Fungi Research Center, and Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

A total of 105 independent Candida albicans strains isolated from patients in Iran were investigated. According to CLSI documents M27-A3 and M27-S4, the 24 h geometric mean MICs of caspofungin, itraconazole, and fluconazole were 0.27, 3.19, and 11.91 μg/ml, respectively. Microsatellites analysis of CEF3, CAIII, LOC4 Loci identified 93 different allelic genotypes clustered apart into six different clades. Antifungal susceptibility was not linked with the source of isolation and the corresponding genotype of C. albicans strains.
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http://dx.doi.org/10.1093/mmy/myy016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506605PMC
January 2019

Molecular identification and antifungal susceptibility profile of species isolated from patients with vulvovaginitis in Tehran, Iran.

J Res Med Sci 2017 26;22:132. Epub 2017 Dec 26.

Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.

Background: Rapid and accurate identification and evaluation of antifungal susceptibility pattern of isolates are crucial to determine suitable antifungal drugs for the treatment of patients with vulvovaginitis candidiasis.

Materials And Methods: Vaginal samples were collected from 150 women with suspicious vaginal candidiasis, and then cultured on Sabouraoud's Dextrose Agar with chloramphenicol to isolate species. After identification of isolates using polymerase chain reaction-restriction fragment length polymorphism technique, antifungal susceptibility testing of four azolic antifungal drugs was carried out using broth microdilution method according to the CLSI M27-A3.

Results: species were isolated from eighty suspected patients (61.79%). The most common pathogen was (63.75%). Resistance to fluconazole and ketoconazole was observed in 27.5% and 23.75% of isolates, respectively, and only 2% of isolates were resistant to miconazole. Interestingly, resistance to fluconazole in was more than other species.

Conclusion: The results indicated that therapy should be selected according to the antifungal susceptibility tests for the prevention of treatment failure and miconazole therapy can be considered as the best therapeutic choice in the management of vulvovaginitis.
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http://dx.doi.org/10.4103/jrms.JRMS_106_17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5767810PMC
December 2017

Multi-Gene Expression in Anthracosis of the Lungs as One of the Risk Factors for Non-Small Cell Lung Cancer

Asian Pac J Cancer Prev 2017 11 26;18(11):3129-3133. Epub 2017 Nov 26.

Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran. Email:

Background: Anthracosis of the lung occurs due to the deposition of carbon and silica in the mucosa and submucosa, manifested as black lesions. The association of anthracosis with lung cancer has remained to be clearly elucidated The current study aimed to assess the P16, CDH1 and LUNX genes expression level to evaluate the association of anthracotic lesions in the lungs with the occurrence of non-small cell lung cancer. Methods: Forty biopsy samples were taken from the center and 40 from the margins of black anthracotic lesions in the lungs; RNA was extracted from the samples and cDNA was synthesized. Real-time reverse-transcription polymerase chain reaction (RT-PCR) was performed to assess the expression of P16, CDH1 and LUNX genes. All steps were performed in triplicate. Results: A significant reduction in P16 gene expression was noted at the center compared to the margins of the lesions (P<0.001). expression level of CDH1 at the center of lesions was significantly lower than margins (P<0.001). However, LUNX gene had significantly higher expressionlevel at the center compared to margins (P<0.001). Conclusion: Decreased expression of P16 and CDH1 and increased expression of LUNX tumor genes were noted at the center of anthracotic lesions. Significant increase in expression of LUNX gene in NSCLC indicates an association between anthracosis and NSCLC, according to which, anthracotic patients may carry a high risk for NSCLC.
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http://dx.doi.org/10.22034/APJCP.2017.18.11.3129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773802PMC
November 2017

Effects of Aspirin as an Anti-inflammatory Drug on Azole-resistant In Vitro.

Iran J Public Health 2016 Nov;45(11):1523-1524

Division of Molecular Biology, Dept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5182267PMC
November 2016

Effects of on Growth and Aflatoxin Production by .

Jundishapur J Microbiol 2016 Jul 29;9(7):e35452. Epub 2016 May 29.

Department of Medical Mycology and Parasitology, Kurdistan University of Medical Sciences, Sanandaj, IR Iran.

Background: Aflatoxins are highly toxic secondary metabolites mainly produced by . This species can contaminate a wide range of agricultural commodities, including cereals, peanuts, and crops in the field. In recent years, research on medicinal herbs, such as , have led to reduced microbial growth, and these herbs also have a particular effect on the production of aflatoxins as carcinogenic compounds.

Objectives: In this study, we to examine as a natural compound used to inhibit the growth of and to act as an anti-mycotoxin.

Materials And Methods: In vitro antifungal susceptibility testing of for was performed according to CLSI document M38-A2. The rate of aflatoxin production was determined using the HPLC technique after exposure to different concentrations (62.5 - 125 mg/mL) of the gum. The changes in expression levels of the gene were analyzed with a quantitative real-time PCR assay.

Results: The results showed that can inhibit growth at a concentration of 125 mg/mL. HPLC results revealed a significant decrease in aflatoxin production with 125 mg/mL of , and AFL-B1 production was entirely inhibited. Based on quantitative real-time PCR results, the rate of gene expression was significantly decreased after treatment with .

Conclusions: has anti-toxic properties in addition to an inhibitory effect on growth, and is able to decrease aflatoxin production effectively in a dose-dependent manner. Therefore, this herbal extract maybe considered a potential anti-mycotoxin agent in medicine or industrial agriculture.
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http://dx.doi.org/10.5812/jjm.35452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5076382PMC
July 2016

Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care Unit.

Adv Med 2016 23;2016:9242031. Epub 2016 Aug 23.

Department of Medical Parasitology and Mycology, Kurdistan University of Medical Sciences, P.O. Box 14155-6446, Sanandaj, Iran.

Aims. Biofilms formed by Candida species which associated with drastically enhanced resistance against most antimicrobial agents. The aim of this study was to identify and determine the antifungal susceptibility pattern of Candida species isolated from endotracheal tubes from ICU patients. Methods. One hundred forty ICU patients with tracheal tubes who were intubated and mechanically ventilated were surveyed for endotracheal tube biofilms. Samples were processed for quantitative microbial culture. Yeast isolates were identified to the species level based on morphological characteristics and their identity was confirmed by PCR-RFLP. Antifungal susceptibility testing was determined according to CLSI document (M27-A3). Results. Ninety-five strains of Candida were obtained from endotracheal tubes of which C. albicans (n = 34; 35.7%) was the most frequently isolated species followed by other species which included C. glabrata (n = 24; 25.2%), C. parapsilosis (n = 16; 16.8%), C. tropicalis (n = 12; 12.6%), and C. krusei (n = 9; 9.4%). The resulting MIC90 for all Candida species were in increasing order as follows: caspofungin (0.5 μg/mL); amphotericin B (2 μg/mL); voriconazole (8.8 μg/mL); itraconazole (16 μg/mL); and fluconazole (64 μg/mL). Conclusion. Candida species recovered from endotracheal tube are the most susceptible to caspofungin.
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http://dx.doi.org/10.1155/2016/9242031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5011531PMC
September 2016

In Vitro Antifungal Susceptibility Profiles of Candida albicans Complex Isolated from Patients with Respiratory Infections.

Acta Med Iran 2016 Jun;54(6):376-81

Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Candidiasis, the main opportunistic fungal infection has been increased over the past decades. This study aimed to characterize C.albicans species complex (C.albicans, C.dubliniensis, and C.africana) isolated from patients with respiratory infections by molecular tools and in vitro antifungal susceptibilities by using broth microdilution method according to CLSI M27-A3 guidelines. Totally, 121 respiratory samples were collected from patients with respiratory infections. Of these, 83 strains were germ tube positive and green colonies on chromogenic media, so initially identified as C.albicans species complex and subsequently were classified as C.albicans (89.15%), C.dubliniensis (9.63%), and C.africana (1.2%) based on PCR-RFLP and amplification of hwp1 gene. Minimum inhibitory concentration (MICs) results showed that all tested isolates of C.albicans complex were highly susceptible to triazole drugs. However, caspofungin had highest activity against C.albicans, C.dubliniensis, and C.africana. Our findings indicated the variety of antifungal resistance of Candida strains in different areas. These results may increase the knowledge about the local distribution of the mentioned strains as well as their antifungal susceptibility pattern which play an important role in appropriate therapy.
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June 2016

Arthrocladium, an unexpected human opportunist in Trichomeriaceae (Chaetothyriales).

Fungal Biol 2016 Feb 5;120(2):207-18. Epub 2015 Sep 5.

Department of Basic Pathology, Federal University of Paraná State, 81540-970, Curitiba, Paraná, Brazil; CBS-KNAW Fungal Biodiversity Centre, PO Box 85167, 3508 AD, Utrecht, The Netherlands; Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, PO Box 94248, 1090 GE, Amsterdam, The Netherlands; Shanghai Institute of Medical Mycology, Changzheng Hospital, Second Military Medical University, 200003, ​Shanghai, China; Biological Sciences Department, Faculty of Science, King Abdulaziz University, PO Box 80203, ​Jeddah, Saudi Arabia. Electronic address:

The family Trichomeriaceae (Chaetothyriales) mainly comprises epiphytic and epilithic organisms. In some species elaborate ascomata are formed, but for the great majority the species no asexual conidium formation is known other than simple fragmentation of the thallus. The present paper re-establishes the genus Arthrocladium with three non-sporulating species. One of these is described for a strain causing a fatal infection in a human patient with a rare genetic immune disorder.
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http://dx.doi.org/10.1016/j.funbio.2015.08.018DOI Listing
February 2016