Publications by authors named "Somayeh Gharaie Fathabad"

5 Publications

  • Page 1 of 1

Impact of Zero-Valent Iron Nanoparticles on Transesterified Lipids and Fatty Acid Methyl Esters.

ACS Omega 2020 Jun 20;5(21):12166-12173. Epub 2020 May 20.

Department of Biology, Morgan State University, 1700 East Cold Spring Lane, Baltimore, Maryland 21251, United States.

Efforts to enhance the transformative potential of biofuels is an important step to achieving an environment-friendly and sustainable energy source. is an ideal third-generation biofuel agent due to its ability to produce lipids and desirable essential fatty acids. In this study, the impact of Nanofer 25s nanoscale zero-valent iron nanoparticles (nZVIs) on total lipid content and fatty acid composition of strains SF33 and B481 was investigated. We observed significant increases ( < 0.05) in the growth of treated with 0.2-1.6 mg L Nanofer 25s, indicating that trace concentrations of nZVIs were not toxic to the organism. Chlorophyll , carotenoids, and phycobiliprotein levels were not altered in treated with nZVIs ranging from 0.4 to 1.6 mg L, confirming that these concentrations did not negatively impact photosynthetic efficacy. In addition, Nanofer 25s ranging from 0.2 to 1.6 mg L had an optimal impact on SF33 and B481 total lipid content. We identified significant increases in unsaturated fatty acid methyl esters (FAMEs) from Nanofer 25s-treated transesterified lipids. Theoretical chemical and physical biofuel properties revealed a product with elevated cetane number and oxidative stability for both strains. Scanning electron microscopy and energy-dispersive X-ray spectroscopy validated the localization of nZVIs. Our findings indicate that Nanofer 25s nZVIs significantly enhance total lipid content and essential FAMEs, thus offering a promising approach to augment the potential of the cyanobacterium as a large-scale biofuel agent.
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http://dx.doi.org/10.1021/acsomega.0c00566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271364PMC
June 2020

T Lymphocytes in Acute Kidney Injury and Repair.

Semin Nephrol 2020 03;40(2):114-125

Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, MD. Electronic address:

Innate and adaptive immune systems participate in the pathogenesis of acute kidney injury (AKI). Considerable data from different research teams have shown the importance of T lymphocytes in the pathophysiology of AKI and, more recently, prevention and repair. T cells can generate or resolve inflammation by secreting specific cytokines and growth factors as well as interact with other immune and stromal cells to induce kidney injury or promote tissue repair. There also are emerging data on the role of T cells in the progression of AKI to chronic kidney disease and organ cross-talk in AKI. These data set the stage for immunomodulatory therapies for AKI. This review focuses on the major populations of T lymphocytes and their roles as mediators for AKI and repair.
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http://dx.doi.org/10.1016/j.semnephrol.2020.01.003DOI Listing
March 2020

Microcystin Levels in Selected Cyanobacteria Exposed to Varying Salinity.

J Water Resour Prot 2019 Apr;11(4):395-403

Department of Biology, Morgan State University, Baltimore, MD, USA.

Microcystins produced by cyanobacteria pose a great threat to human health by releasing toxins upon cell death. In the present study, we studied microcystin production in the cyanobacterial strains (B629 and 2949) and (SF33) exposed to 1, 2 and 4 g/L sodium chloride (NaCl). Cultures grown for 7 days in BG11/HEPES medium were pelleted, re-grown in the corresponding NaCl levels, and enzyme linked immunosorbent assay (ELISA) performed. ELISA assays revealed enhanced microcystin production in B629 exposed to 4 g/L NaCl and 29414 exposed to 2 and 4 g/L NaCl, after growth in the corresponding NaCl levels for 14 days. We observed a significant decrease (p >0.05) in microcystin levels in the control strains after exposure to NaCl for 5 days. After exposure to 1, 2, or 4 g/L NaCl for 10 days, no microcystin release was observed in B629, 29414 or F. SF33. Sodium dodecyl sulfate polyacrylamide gel electrophoresis identified the presence of an additional band at 120 - 130 kDa in B629 exposed to 2 and 4 g/L NaCl, and at 14 kDa in cultures amended with 1 and 2 g/L NaCl as well as the untreated control, indicating that exposure to salinity induces alterations in protein expression.
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http://dx.doi.org/10.4236/jwarp.2019.114023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010315PMC
April 2019

Nanoparticle-mediated Impact on Growth and Fatty Acid Methyl Ester Composition in the Cyanobacterium .

Bioenergy Res 2019 Jun 8;12:409-418. Epub 2019 Mar 8.

Department of Biology, Morgan State University, 1700 East Cold Spring Lane, Baltimore, Maryland 21251, United States.

Insufficient light supply is a major limitation in cultivation of cyanobacteria for scaled up biofuel production and other biotechnological applications, which has driven interest in nanoparticle-mediated enhancement of cellular light capture. In the present study, wild type (Fd33) and halotolerant (HSF33-2) strains were grown in solution with 20, 100, and 200 nm-diameter gold nanoparticles (AuNPs) to determine their impact on biomass accumulation, pigmentation, and fatty acid methyl ester (FAME) production. Results revealed a significant increase in growth of Fd33 (0.244 ± 0.006) and HSF33-2 (0.112 ± 0.003) when treated with 200 nm AuNPs. In addition, we observed a significant increase in chlorophyll accumulation in 200 nm AuNP-treated Fd33 (25.7%) and HSF33-2 (36.3%) indicating that NPs enhanced photosynthetic pigmentation. We did not observe any alteration in FAME composition and biodiesel properties of transesterified lipids among all AuNP treatments. Interactions between and AuNPs were visualized using scanning electron microscopy. Energy dispersive X-ray spectroscopy confirmed the presence of AuNPs outside cells with aggregation in high cell density locales. Our findings indicate that nanotechnological approaches could significantly enhance growth of the organism with no negative effect on FAME-derived biodiesel properties, thus augmenting as a potential biofuel agent.
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http://dx.doi.org/10.1007/s12155-019-09966-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6980354PMC
June 2019

Augmenting Fremyella diplosiphon Cellular Lipid Content and Unsaturated Fatty Acid Methyl Esters Via Sterol Desaturase Gene Overexpression.

Appl Biochem Biotechnol 2019 Dec 6;189(4):1127-1140. Epub 2019 Jun 6.

Department of Biology, Morgan State University, 1700 East Cold Spring Lane, Baltimore, MD, 21251, USA.

Cyanobacteria have immense prospective as a platform for renewable energy; however, a major barrier in achieving optimal productivity is the low lipid yield. Fremyella diplosiphon, a model cyanobacterium, is an ideal biofuel agent due to its desirable fatty acid methyl esters (FAMEs). To enhance lipid content, we overexpressed the sterol desaturase (SD) gene in F. diplosiphon B481 wild type by genetic transformation. This effort resulted in a transformant (B481-SD) with a 64-fold increase in the SD gene at the mRNA transcript level, with no loss in growth and pigmentation. The transformant was persistently grown for over 32 generations indicating long-term stability and vitality. We observed 27.3% and 23% increases in total lipid content and unsaturated FAMEs respectively in B481-SD transesterified lipids with methyl octadecadienoate as the most abundant unsaturated component. In addition, we detected an 81% increase in FAME composition in the transformant compared with the wild type. Theoretical physical and chemical properties confirmed a FAME profile with very high cetane number (65.972-67.494) and oxidative stability (50.493-18.66 h) in the engineered strain. Results of the study offer a promising approach to augment F. diplosiphon total lipid content and unsaturated FAMEs, thus paving the way to enhance biofuel capacity of the organism.
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http://dx.doi.org/10.1007/s12010-019-03055-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884679PMC
December 2019