Publications by authors named "Soheila Moein"

16 Publications

  • Page 1 of 1

Signaling, metabolism, and cancer: An important relationship for therapeutic intervention.

J Cell Physiol 2021 Feb 12. Epub 2021 Feb 12.

Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

In cancerous cells, significant changes occur in the activity of signaling pathways affecting a wide range of cellular activities ranging from growth and proliferation to apoptosis, invasiveness, and metastasis. Extensive changes also happen with respect to the metabolism of a cancerous cell encompassing a wide range of functions that include: nutrient acquisition, biosynthesis of macromolecules, and energy generation. These changes are important and some therapeutic approaches for treating cancers have focused on targeting the metabolism of cancerous cells. Oncogenes and tumor suppressor genes have a significant effect on the metabolism of cells. There appears to be a close interaction between metabolism and the signaling pathways in a cancerous cell, in which the interaction provides the metabolic needs of a cancerous cell for uncontrolled proliferation, resistance to apoptosis, and metastasis. In this review, we have reviewed the latest findings in this regard and briefly review the most recent research findings regarding targeting the metabolism of cancer cells as a therapeutic approach for treatment of cancer.
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http://dx.doi.org/10.1002/jcp.30276DOI Listing
February 2021

Association between serum folate with inflammatory markers, disease clinical activity and serum homocysteine in patients with inflammatory bowel disease. Does folate level have an effect on maintaining clinical remission?

Acta Biomed 2020 11 10;91(4):e2020106. Epub 2020 Nov 10.

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Background Folate is an important vitamin with protective effect against some human diseases. The aim of this study was to evaluate the relationship between serum folate levels, inflammatory markers and disease clinical activity in patients with inflammatory bowel disease (IBD).   Methods The participants were classified into two groups in which 38 IBD patients and 38 healthy controls were studied. Disease clinical activities were evaluated by means of established score systems. Serum folate, homocysteine and C-reactive protein and ESR were measured. Obtained data were analyzed with proper statistical methods and P- value less than 0.05 was considered as statistical significant.   Results The level of serum folate was significantly reduced in IBD patients with active disease compared to patients with clinical remission (p=0.043) and also healthy controls (p = 0.008). Moreover, there was a significant inverse correlation between serum folate levels and C-reactive protein in IBD patients (r = -0.563 p =0.001).         Conclusion Serum folate levels is associated with inflammatory markers and disease clinical activity in IBD patients, therefore there is a possibility that disease clinical activity is reduced with adequate folate level.
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http://dx.doi.org/10.23750/abm.v91i4.8467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927538PMC
November 2020

Evaluation of MASP1, CMPF and U.A serum levels in pre-diabetic subjects in comparison to Normal individuals for early diagnosis of subjects with pre-diabetes, a case-control study.

J Diabetes Metab Disord 2020 Dec 21;19(2):1593-1598. Epub 2020 Nov 21.

Department of Biochemistry, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

Background: The present study designed to evaluate the Serum levels of CMPF, MASP1 and UA in pre-diabetic subjects was compared to normal subjects

Methods: This research is a case-control study. We studied 44 pre-diabetics with 44 normal subjects and were evaluated serum concentration of CMPF, Masp1 and U.Ain both groups andfurthermore serum concentration FPG, BUN, Cr, Cho, TG, HDLc, LDLc, AST, ALT, ALP, HbA1c was examined and correlation between of CMPF, Masp1 and U.Aand other parameters was statistically analyzed.

Results: Serum levels of MASP1, CMPF, fasting plasma glucose ( < 0.001)and uric acid ( < 0.002) were significantly increased in pre-diabetic subjects. In this study, a significant difference was found between MASP1 and CMPF in pre-diabetic subjects compared to normal subjects (P=0.005, r=0.291). There was also a significant difference between serum levels of MASP1 with HbA1C (P=0.01, r=0.269).

Conclusion: Serum levels of CMPF, MASP1 and uric acid were increased in pre-diabetic subjects. These parameters can be used as a biomarker for the diagnosis of pre-diabetes.
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http://dx.doi.org/10.1007/s40200-020-00697-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843784PMC
December 2020

Sensitivity and specificity of mean platelet volume as a laboratory marker for irritable bowel syndrome: Can it be added to Rome criteria?

Afr J Lab Med 2020 21;9(1):1001. Epub 2020 Dec 21.

Department of Biostatistics and Epidemiology, Babol University of Medical Sciences, Babol, Iran.

Background: Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder.

Objective: This study aimed to evaluate red blood cell distribution width (RDW) and mean platelet volume (MPV) as laboratory markers to discriminate IBS patients from both healthy controls and patients with inflammatory bowel disease (IBD).

Methods: This case-control study enrolled patients referred to Ayatollah Rouhani Hospital, Endoscopy Department, Babol, Iran, for colonoscopy examination from 2015 to 2017. Fifty IBS patients were selected from among patients who had undergone a normal colonoscopy and showed symptoms matching the Rome III criteria. Fifty healthy participants and 50 IBD patients, matched for sex and age, were also enrolled in this study. Both RDW and MPV were measured and analysed by independent sample -test and receiver operating characteristic curve analysis. A -value of less than 0.05 was considered statistically significant.

Results: While RDW was higher and MPV was lower among IBS patients compared to healthy controls ( = 0.047 and = 0.001), there were no significant differences in RDW or MPV levels between IBS and IBD patients. The area under the curve of RDW in the discrimination between IBS and IBD was 0.620 ( = 0.039), and the area under the curve of MPV in the discrimination between healthy controls and IBS patients was 0.801 ( = 0.001).

Conclusion: Mean platelet volume is potentially a useful laboratory marker for distinguishing between IBS patients and healthy individuals. Red blood cell distribution width should be considered as a potential marker to distinguish among IBS and IBD patients.
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http://dx.doi.org/10.4102/ajlm.v9i1.1001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756523PMC
December 2020

MicroRNAs and colorectal cancer chemoresistance: New solution for old problem.

Life Sci 2020 Oct 17;259:118255. Epub 2020 Aug 17.

Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

Background: Colorectal cancer (CRC) is one of the most common gastrointestinal malignancies with a significant mortality rate. Despite the great advances in cancer treatment in the last few decades, effective treatment of CRC is still under challenge. One of the main problems associated with CRC treatment is the resistance of cancer cells to chemotherapy drugs.

Methods: Many studies have been carried out to identify CRC chemoresistance mechanisms, and shed light on the role of ATP-binding cassette transporters (ABC transporters), enzymes as thymidylate synthase, some signaling pathways, and cancer stem cells (CSC) in chemoresistance and failed CRC chemotherapies. Other studies have also been recently carried out to find solutions to overcome chemoresistance. Some of these studies have identified the role of miRNAs in chemoresistance of the CRC cells and the effective use of these micro-molecules to CRC treatment.

Results: Considering the results of these studies, more focus on miRNAs likely leads to a proper solution to overcome CRC chemoresistance.

Conclusion: The current study has reviewed the related literature while discussing the efficacy of miRNAs as potential clinical tools for overcoming CRC chemoresistance and reviewing the most important chemoresistance mechanisms in CRC cells.
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http://dx.doi.org/10.1016/j.lfs.2020.118255DOI Listing
October 2020

Calprotectin in inflammatory bowel disease.

Clin Chim Acta 2020 Nov 18;510:556-565. Epub 2020 Aug 18.

Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

The term IBD is usually used for referring to a group of inflammatory gastro-intestinal diseases (mainly Crohn's disease and ulcerative colitis). Accordingly, IBD arises as a result of inappropriate immune response to intestinal commensal organisms among genetically susceptible individuals. Performing colonoscopy and histopathologic evaluation on an inflamed bowel biopsy specimen are currently considered as gold standards for diagnosis and management of IBD. Correspondingly, these techniques are known to be invasive and costly. In recent decades, fecal calprotectin, as a biomarker, has received much attention for the diagnosis and non-invasive management of IBD. Up to now, many studies have investigated the efficacy of fecal calprotectin in the areas of IBD differentiation from IBS, prediction of endoscopic and histologic activities of IBD and prediction of disease recurrence. Although some of these studies have reported promising results, some others have shown significant limitations. Therefore, in this paper, we reviewed the most interesting ones of these studies after a brief discussion of the laboratory measurement of fecal calprotectin. Moreover, we attempted to provide an answer for the question of whether fecal-calprotectin could be considered as a potential surrogate marker for colonoscopy.
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http://dx.doi.org/10.1016/j.cca.2020.08.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431395PMC
November 2020

MiRNAs and inflammatory bowel disease: An interesting new story.

J Cell Physiol 2019 04 11;234(4):3277-3293. Epub 2018 Nov 11.

Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Inflammatory bowel disease (IBD), as a chronic and recurrent inflammatory disorder, is caused by a dysregulated and aberrant immune response to exposed environmental factors in genetically susceptible individuals. Despite huge efforts in determining the molecular pathogenesis of IBD, an increasing worldwide incidence of IBD has been reported. MicroRNAs (miRNAs) are a set of noncoding RNA molecules that are about 22 nucleotides long, and these molecules are involved in the regulation of the gene expression. By clarifying the important role of miRNAs in a number of diseases, their role was also considered in IBD; numerous studies have been performed on this topic. In this review, we attempt to summarize a number of studies and discuss some of the recent developments in the roles of miRNAs in the pathophysiology, diagnosis, and treatment of IBD.
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http://dx.doi.org/10.1002/jcp.27173DOI Listing
April 2019

Alleviating the progression of acute myeloid leukemia (AML) by sulforaphane through controlling miR-155 levels.

Mol Biol Rep 2018 Dec 22;45(6):2491-2499. Epub 2018 Oct 22.

Department of Medical Genetic, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

Acute myeloid leukemia (AML) has the highest rate of mortality among the leukemias. Disruption in miRNAs level is involved in the pathogenesis of the disease. The miR-155 has a role in primary differentiation of myeloid progenitor. Meanwhile, there is little knowledge about the effects of sulforaphane against leukemia. The present study tried to evaluate pathologic effect of miR-155 in patients in various subgroups of AML, and then pioneered in assessing miR-155 levels by the effect of sulforaphane in different AML cell lines. The miR-155 level was significantly higher in patients with AML compared to the controls. Interestingly, the increase in miR-155 was converged with raising the subtype of AML (from M1 to M5). The miR-155 levels increased by 1.2 times in patients with M1, but this increase reached 2.5 times in the patients in the M5 subgroup. Sulforaphane reduced the number of live cells and increased the mortality rate of AML cells particularly by induction of apoptosis. However, the anti-proliferative effect of this agent was more dominant and could dose-dependently lessen miR-155 levels in myeloid leukemia cells. More or less, about 80% reduction in miR-155 expression was almost observed after 48 h treatment with 60 µM sulforaphane in all four studied cell lines. The obtained results indicated that miR-155 might function as an oncomir in AML and can potentially be considered as a prognosis biomarker for AML. The anti-cancer effects of sulforaphane can be correlated with reduction of miR-155 levels. These findings suggested that sulforaphane could induce more differentiation in myeloid progenitor cells through controlling the miR-155, thereby mitigating the progress of AML.
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http://dx.doi.org/10.1007/s11033-018-4416-0DOI Listing
December 2018

Positive Correlation of Fecal Calprotectin With Serum Antioxidant Enzymes in Patients With Inflammatory Bowel Disease: Accidental Numerical Correlation or a New Finding?

Am J Med Sci 2018 05 27;355(5):449-455. Epub 2017 Dec 27.

Departments of Biostatistics and Epidemiology, Babol University of Medical Sciences, Babol, Iran.

Background: Oxidative stress occuring in patients diagnosed with inflammatory bowel disease (IBD), but the relationship between oxidative stress, disease activity and inflammatory markers has not been well established.

Materials And Methods: A total of 30 patients diagnosed with IBD and 30 volunteers who had normal colonoscopies, selected as controls, were used for this study. The serum levels of antioxidant enzymes (catalase and glutathione peroxidase) and oxidative markers (malondialdehyde [MDA] and total antioxidant capacity) were compared between the 2 groups. Furthermore, their correlations with disease activity scores and inflammatory markers, especially the fecal calprotectin, were examined.

Results: Catalase and glutathione peroxidase concentrations were significantly correlated with the level of fecal calprotectin in patients with IBD. Nevertheless, there were no significant correlations between the concentrations of the above-mentioned enzymes and C-reactive protein, erythrocyte sedimentation rate or the activity scores of IBD patients. It should be noted that MDA and total antioxidant capacity levels did not correlate with the inflammatory markers or the disease activity scores.

Conclusions: There was a positive correlation between fecal calprotectin and serum antioxidant enzymes in patients with IBD, but, there was no correlation between antioxidant and oxidative markers in terms of disease activity scores. Hence, the observed significant correlation between the antioxidant enzymes and the fecal calprotectin may be due to either the pro-oxidant potential of calprotectin or its antioxidant role.
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http://dx.doi.org/10.1016/j.amjms.2017.12.009DOI Listing
May 2018

Direct correlation between serum homocysteine level and insulin resistance index in patients with subclinical hypothyroidism: Does subclinical hypothyroidism increase the risk of diabetes and cardio vascular disease together?

Diabetes Metab Syndr 2018 Nov 5;12(6):863-867. Epub 2018 May 5.

Department of Endocrinology, Internal Medicine, Ayatollah Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran.

Background: Subclinical hypothyroidism known as mild thyroid disorder without significant sign and symptoms. The correlation between subclinical hypothyroidism and some of cardiovascular disease risk factors such as serum lipids, homocysteine levels and also insulin resistance index is not well established and the current study was conducted to clarify this issue.

Methods And Materials: Seventy four patients with mild elevation in levels of thyroid stimulating hormone (TSH) along with normal levels of T3 and T4 were selected as patients group and 74 age and sex matched individuals were selected as healthy control group. Serum insulin, triglyceride, glucose, total cholesterol, LDL-cholesterol, HDL-cholesterol and homocysteine levels were measured. Obtained data compared between groups with independent sample t-test. For evaluation of the correlation between mentioned parameters Pearson correlation coefficient method was used.

Results: Serum levels of LDL-C and total cholesterol significantly increased in SCH group compared to healthy control group. Homeostatic Model Assessment of Insulin Resistance (HOM-IR) and serum homocysteine level significantly elevated in patients with SCH compared to control group. There was a significant direct correlation between HOM-IR and serum homocysteine levels in SCH patients.

Conclusion: Subclinical hypothyroidism likely have significant effect on insulin resistance as major diabetes risk factors and also cardiovascular disease risk factors such as homocysteine. The direct correlation between HOM-IR with serum homocysteine level indicate the possible role of insulin resistance in elevation of serum homocysteine in SCH patient group.
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http://dx.doi.org/10.1016/j.dsx.2018.05.002DOI Listing
November 2018

Evaluation of Antioxidant Potentials and -Amylase Inhibition of Different Fractions of Labiatae Plants Extracts: As a Model of Antidiabetic Compounds Properties.

Biomed Res Int 2017 10;2017:7319504. Epub 2017 Sep 10.

Department of Biochemistry, Faculty of Basic Sciences, Islamic Azad University of Shiraz, Sadra City, Fars, Iran.

In an attempt to identify herbal drugs which may become useful in the prevention of diabetes, antioxidant potentials and -amylase inhibition by the ethanol extracts of two plants belonging to Lamiaceae family, and , and their different fractions were studied. Also, inhibition of -amylase by and its fractions was evaluated. All of the samples exhibited antioxidant activities, among which ethyl acetate fraction of (17.21 ± 0.17 mg GAE/g) was found to contain the highest amounts of phenols and the ethyl acetate fraction of (218 ± 2.76 mg QUE/g) had the most values of flavonoids. Ethyl acetate fraction of (IC = 3.05 ± 0.51 g/ml) was shown to have the most reducing power and the ethyl acetate fraction of (IC = 32.17 ± 1.82 g/ml) exhibited the highest DPPH radical scavenging. The ethyl acetate fraction of (99.39 ± 0.94%) showed the highest -amylase inhibitory activity which was similar to acarbose used as a standard. Mode of -amylase inhibition of the most samples was uncompetitive except for , , , and which presented competitive inhibition. The present findings showed that studied samples may have some compounds with antioxidant and antidiabetic effects.
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http://dx.doi.org/10.1155/2017/7319504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5610887PMC
June 2018

Diagnostic accuracy of fecal calprotectin in assessing the severity of inflammatory bowel disease: From laboratory to clinic.

Caspian J Intern Med 2017 ;8(3):178-182

Social Determinant of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol,Iran.

Background: Inflammatory bowel disease (IBD) involves chronic inflammation of the digestive tract. In the past decades, fecal calprotectin has been proposed as a useful biomarker for the differential diagnosis between IBD patients and healthy controls. We designed this study to evaluate the diagnostic ability of fecal calprotectin (FC) and conventional inflammatory markers in IBD patients.

Methods: Thirty patients who underwent colonoscopy were cases and thirty as part of a medical check-up were the controls. These 2 evaluated with regard to  and . Severity of the disease was evaluated based on disease endoscopic index. FC, Cross reactive protein (CRP) and Erythrocyte sedimentation rate (ESR) were measured using ELISA, colorimetric and Westergren methods, respectively. The obtained data were analyzed by independent test, correlation test and receiver operating characteristic (ROC) curve analysis. A p<0.05 was considered statistically significant.

Results: Fecal calprotectin level in the case group increased compared to control group (p<0.05). Moreover FC has stronger correlation with disease endoscopic activity than conventional inflammatory markers (r=0.847 versus r= -0.44 for CRP and r=0.054 for ESR in Crohn's disease and r=0.798 versus r=0.463 for CRP and r=0.467 for ESR in ulcerative colitis). Receiver operating characteristic (ROC) curve analysis showed FC has larger area under the curve (AUC) than conventional inflammatory markers (1 versus 0.849 for CRP and 0.846 for ESR).

Conclusion: Discriminating IBD patients from healthy controls was better for FC than conventional inflammatory markers. Additionally, the results produced by FC correlate with the severity of IBD.
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http://dx.doi.org/10.22088/cjim.8.3.178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596188PMC
January 2017

Kinetics of α-glucosidase inhibition by different fractions of three species of Labiatae extracts: a new diabetes treatment model.

Pharm Biol 2017 Dec;55(1):1483-1488

d Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences , Shiraz , IR Iran.

Context: Glucosidases are a group of enzymes playing crucial roles in digestion of carbohydrates. Glucosidase inhibitors can reduce carbohydrate digestion rate and have the potential to prevent development of type 2 diabetes. The Labiatae is one of the largest plant families grown globally and many studies that have isolated new pharmaceutical compounds. In folk medicine, some of Labiatae plants such as Zataria multiflora Boiss, Salvia mirzayanii Rech. F. & Esfand, and Otostegia persica Boiss are consumed for the treatment of diabetes.

Objectives: This study investigates the inhibitory effects of different fractions of three mentioned species extracts on α-glucosidase.

Materials And Methods: Ethanol extracts of these plants leaves were fractionated using petroleum ether, chloroform, ethyl acetate, and n-butanol solutions. The duration of this study was 12 months. To measure enzyme inhibition, 5 μL of the enzyme, 20 μL of substrate and samples were used and for evaluation mode of inhibition, constant amounts of α-glucosidase were incubated with rising concentrations of substrate (PNPG).

Results: The results revealed that the ethyl acetate fraction of Zataria multiflora (IC = 0.35 ± 0.01 mg/mL) and petroleum ether fraction of Salvia mirzayanii (IC = 0.4 ± 0.11 mg/mL) were the most potent inhibitors of α-glucosidase in comparison with the other samples and acarbose as the standard (IC = 7 ± 0.19 mg/mL). All of the samples exhibited noncompetitive-uncompetitive inhibition.

Discussion And Conclusion: It can be inferred from this study that α-glucosidase inhibitory potential of the studied extracts may be a marker of antidiabetic potential of these extracts.
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http://dx.doi.org/10.1080/13880209.2017.1306569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011978PMC
December 2017

Evaluation of alpha- amylase inhibition by Urtica dioica and Juglans regia extracts.

Iran J Basic Med Sci 2014 Jun;17(6):465-9

Medicinal Plant Processing Research Center and Department of Pharmacognosy, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.

Objectives: One strategy for the treatment of diabetes is inhibition of pancreatic α- amylase. Plants contains different chemical constituents with potential for inhibition of α-amylase and hence maybe used as therapeutic.

Materials And Methods: Urtica dioica and Juglans regia Linn were tested for α-amylase inhibition. Different concentrations of leaf aqueous extracts were incubated with enzyme substrate solution and the activity of enzyme was measured. For determination of the type of inhibition, Dixon plot was depicted. Acarbose was used as the standard inhibitor.

Results: Both plant extracts showed time and concentration dependent inhibition of α-amylase. 60% inhibition was seen with 2 mg/ml of U. dioica and 0.4 mg/ml of J. regia aqueous extract. Dixon plots revealed the type of α-amylase inhibition by these two extracts as competitive inhibition.

Conclusion: Determination of the type of α-amylase inhibition by these plant extracts could provide by successful use of plant chemicals as drug targets.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137939PMC
June 2014

In vitro antioxidant properties evaluation of 10 Iranian medicinal plants by different methods.

Iran Red Crescent Med J 2012 Dec 6;14(12):771-5. Epub 2012 Dec 6.

Department of Biochemistry, Faculty of Medicine and Research Center for Molecular Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IR Iran.

Background: There is an interest in finding new and safe antioxidants from natural sources such as medicinal plants.

Objectives: The aim of this study was to evaluate the antioxidant activity of ten Iranian medicinal plants extracts.

Materials And Methods: For antioxidant activity, the radical scavenging activity, reducing power and phenolic contents of ethanol plant extracts were determined. Gallic acid was used as standard reference with well-documented antioxidant activity.

Results: The highest antioxidant activity in terms of DPPH radical scavenging was found in Verbascum sinuatum L. Var (VS) with an IC50 equal to 263.52 ± 5.981 μg/ml and Rosa damascena Mill (RD) with and IC50 equal to 287.9 ± 5.675 μg/ml that are higher than gallic acid (IC50 = 25.32 ± 5.593 μg/ml). The highest antioxidant activity in terms of ferric reducing capacity was also found in Verbascum sinuatum L. Var extracts (in 85.08 ± 8.66 μg/ml concentration with absorbance 0.5). Also, this extract contains the highest phenolic compounds (8.53 ± 0.11 mg/g).

Conclusion: In this study, Verbascum sinuatum L. Var contains the highest level of phenolic compounds may be contribute to higher free radical scavenging activity and reducing power in comparison to the other plant extracts. Therefore this plant is a good candidate as natural antioxidant.
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http://dx.doi.org/10.5812/ircmj.1408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587865PMC
December 2012

Radical scavenging and reducing power of Salvia mirzayanii subfractions.

Molecules 2008 Nov 12;13(11):2804-13. Epub 2008 Nov 12.

Department of Pharmacognosy and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.

In this research, the radical scavenging activity and reducing power of the ethyl acetate fraction and subfractions of Salvia mirzayanii (SM) have been investigated. The plant material was initially extracted with ethanol. The fractionation was carried out using liquid-liquid extraction, then the ethyl acetate fraction, which showed the greatest antioxidant activity, was selected. This fraction was submitted to column chromatography on a Sephadex LH 20 column eluted with pure MeOH to obtain subfractions A-G. No significant differences exist between the IC(50) of Salvia mirzayanii ethyl acetate subfraction C (IC(50) = 37.9 +/- 0.85), F (IC(50) = 40.05 +/- 1.4) and quercetin (38.84 +/- 0.86), (P > 0.05), indicating that the radical scavenging capacity of these two subfractions and quercetin (antioxidant standard) were similar. The reducing power of the ethyl acetate fraction was less than that of all subfractions, except for subfraction A. The greatest amount of phenolic compounds was found in subfraction E (55.23 +/- 4.2) and the lowest in subfraction F (5.23 +/- 0.18). The greatest total flavonoid content was established in subfraction D (1.84 +/- 0.01) and the lowest was in subfraction A (0.108 +/- 0.007).
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http://dx.doi.org/10.3390/molecules13112804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245311PMC
November 2008