Publications by authors named "Siyuan Wang"

216 Publications

Li MTeO (M=Ti, Sn): Mid-Infrared Nonlinear Optical Crystal with Strong Second Harmonic Generation Response and Wide Transparency Range.

Angew Chem Int Ed Engl 2021 Aug 30. Epub 2021 Aug 30.

State Key Laboratory of Crystal Materials, Shandong University, Jinan, Shandong, 250100, China.

Oxide crystals have been widely used in nonlinear optics (NLO) in the ultraviolet-visible and near-infrared regions. Most traditional oxide crystals are restricted to the mid-infrared region due to their narrow transmission window. Hence, attempting to extend infrared cutoff wavelength of oxides has attracted much attention. Herein, we report two new tellurates Li TiTeO (LTT) and Li SnTeO (LST) with broad transparent regions of 0.38-6.72 and 0.38-6.86 μm, respectively, as excellent candidates for mid-infrared NLO applications. Both LTT and LST crystallize in the orthorhombic space group Pnn2. The LTT crystal exhibits intense powder second-order generation efficiency (26×KDP) under the fundamental wavelength of 1064 nm. First-principles calculations and dipole moments were used to illustrate the results of the powder second-harmonic generations based on the crystal structures. Our results provide a novel oxide NLO crystal with a strong SHG and wide transparency range. They also pave a way for the design of new oxide mid-IR NLO crystals.
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http://dx.doi.org/10.1002/anie.202108978DOI Listing
August 2021

CYP2C19 genotype has prognostic value in specific populations following coronary stenting.

Ann Transl Med 2021 Jul;9(13):1066

Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: The prognostic value of the CYP2C19 genotype in post-percutaneous coronary intervention (PCI) patients remains controversial. The recently-published, limited-sample PHARMCLO trial indicates a personalized pharmacogenomic approach may reduce adverse events. This study aimed to determine the prognostic value of CYP2C19 genotypes.

Methods: The original cohort consisted of 10,724 PCI patients in 2013. 756 patients with genotyped CYP2C19 were included in our analysis. The CYP2C19 genotype prognostic value was tested based on different clinical factors. The primary endpoint was major adverse cardio- and cerebro-vascular event (MACCE).

Results: MACCE 2-years post-PCI occurred in 19 patients (17.4%) in poor metabolizers (PM, CYP2C19 *2/*2, *2/*3, *3/*3), 43 patients (12.2%) in intermediate metabolizers (IM, CYP2C19 *1/*2 or *1/*3) and 27 patients (9.2%) in extensive metabolizers (EM, CYP2C19 *1/*1). PM was an independent MACCE predictor compared with EM (HR: 1.960, 95% CI: 1.139-3.372), but the difference between IM and PM was not significant (HR: 1.314, 95% CI: 0.843-2.048). Major bleeding (BARC grade ≥3) was not significantly different between the three groups (2.5% . 2.1% . 0.8%, P=0.133). Subgroup analysis showed that the CYP2C19 genotype prognostic value was present in the following subgroups: male, age >60 years, body mass index (BMI) >24 kg/m, SYNTAX score >15, current smokers, and patients without chronic kidney disease.

Conclusions: Utilizing CYP2C19 genotype to guide post-PCI antiplatelet therapy might be appropriate in patients with the following characteristics: male, age >60 years, BMI >24 kg/m, SYNTAX score >15, current smokers, and non-chronic kidney disease (CKD) patients.
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http://dx.doi.org/10.21037/atm-20-7724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339845PMC
July 2021

Duck Complement Factor H Binds to Outer Membrane Protein Omp24 of .

Avian Dis 2021 06;65(2):261-268

College of Veterinary Medicine, Southwest University, Chongqing 402460, People's Republic of China,

The resistance to serum complement-mediated killing is a vital virulence property of microbial pathogens. Complement factor H (FH) is a key negative regulator of the complement alternative pathway (AP) that prevents formation and accelerates the decay of AP C3 convertase and acts as a cofactor in the inactivation of C3b. Pathogens can recruit host FH through their surface proteins to escape the clearance of the complement system. could also evade the complement system attack to achieve host infection, but the mechanism is still unclear. In this study, the proteins that could interact with FH in host serum were screened and analyzed, and the functions were determined. Affinity chromatography with a Ni-nitrilotriacetic acid Sefinose column and mass spectrometry identified three outer membrane proteins (Omp) of , Omp54, Omp53, and Omp24, as potential FH-binding proteins. We then successfully conducted the prokaryotic expression and polyclonal antibody preparation of three candidate proteins. Indirect immunofluorescence assay showed that three candidate proteins were all present in . The affinity blotting assay, anti-serum-inhibiting assay, and serum bactericidal assay presented evidence that Omp24 could bind FH. Moreover, FH bound to Omp24 was associated with resistance to the alternative pathway and functional for survival in the normal duck serum. These results suggested that Omp24 was a FH-binding protein and the interaction with FH blocked the alternative pathway. Recruitment of complement regulatory proteins may facilitate better resistance to this vital line of host defense.
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http://dx.doi.org/10.1637/0005-2086-65.2.261DOI Listing
June 2021

Curcumin Inhibits Replication of Human Parainfluenza Virus Type 3 by Affecting Viral Inclusion Body Formation.

Biomed Res Int 2021 9;2021:1807293. Epub 2021 Aug 9.

Pathogen Biology and Immunology Laboratory and Laboratory of Tissue and Cell Biology, Experimental Teaching and Management Center, Chongqing Medical University, Chongqing 401331, China.

Human Parainfluenza Virus Type 3 (HPIV3) is one of the main pathogens that cause acute lower respiratory tract infections in infants and young children. However, there are currently no effective antiviral drugs and vaccines. Herein, we found that a natural compound, curcumin, inhibits HPIV3 infection and has antiviral effects on entry and replication of the virus life cycle. Immunofluorescence and western blotting experiments revealed that curcumin disrupts F-actin and inhibits viral inclusion body (IB) formation, thus inhibiting virus replication. Curcumin can also downregulate cellular PI4KB and interrupt its colocalization in viral IBs. This study verified the antiviral ability of curcumin on HPIV3 infection and preliminarily elucidated its influence on viral replication, providing a theoretical basis for antiviral drug development of HPIV3 and other parainfluenza viruses.
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http://dx.doi.org/10.1155/2021/1807293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367592PMC
August 2021

Remote-controllable bone-targeted delivery of estradiol for the treatment of ovariectomy-induced osteoporosis in rats.

J Nanobiotechnology 2021 Aug 18;19(1):248. Epub 2021 Aug 18.

Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Osteoporosis (OP) is a systemic skeletal disease marked by bone mass reduction and bone tissue destruction. Hormone replacement therapy is an effective treatment for post-menopausal OP, but estrogen has poor tissue selectivity and severe side effects.

Results: In this study, we constructed a poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs)-based drug delivery system to co-load 17β estradiol (E) and iron oxide (FeO) together, modified with alendronate (AL) to achieve bone targeting and realize a magnetically remote-controllable drug release. The NPs were fabricated through the emulsion solvent diffusion method. The particle size was approximately 200 nm while the encapsulation efficiency of E was 58.34 ± 9.21%. The NPs were found to be spherical with a homogenous distribution of particle size. The NPs showed good stability, good biocompatibility, high encapsulation ability of E and excellent magnetic properties. The NPs could be effectively taken up by Raw 264.7 cells and were effective in enriching drugs in bone tissue. The co-loaded NPs exposed to an external magnetic field ameliorated OVX-induced bone loss through increased BV/TV, decreased Tb.N and Tb.Sp, improved bone strength, increased PINP and OC, and downregulated CTX and TRAP-5b. The haematological index and histopathological analyses displayed the NPs had less side effects on non-skeletal tissues.

Conclusions: This study presented a remote-controlled release system based on bone-targeted multifunctional NPs and a new potential approach to bone-targeted therapy of OP.
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http://dx.doi.org/10.1186/s12951-021-00976-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371851PMC
August 2021

The Application of Functional Magnetic Resonance Imaging in Type 2 Diabetes Rats With Contrast-Induced Acute Kidney Injury and the Associated Innate Immune Response.

Front Physiol 2021 29;12:669581. Epub 2021 Jun 29.

Department of Radiology, Xiang'an Hospital of Xiamen University, Xiamen, China.

Aims: Contrast-induced acute kidney injury (CI-AKI) is the third most common in-hospital acquired AKI, and its mechanism is not fully clear. Its morbidity increases among populations with chronic kidney disease (CKD), older age, diabetes mellitus (DM), and so on. Immediate and effective noninvasive diagnostic methods are lacking, so CI-AKI often prolongs hospital stays and increases extra medical costs. This study aims to explore the possibility of diagnosing CI-AKI with functional magnetic resonance imaging (fMRI) based on type 2 DM rats. Moreover, we attempt to reveal the immune response in CI-AKI and to clarify why DM is a predisposing factor for CI-AKI.

Methods: A type 2 DM rat model was established by feeding a high-fat and high-sugar diet combined with streptozotocin (STZ) injection. Iodixanol-320 was the contrast medium (CM) administered to rats. Images were obtained with a SIEMENS Skyra 3.0-T magnetic resonance imager. Renal histopathology was evaluated using H&E staining and immunohistochemistry (IHC). The innate immune response was revealed through western blotting and flow cytometry.

Results: Blood oxygenation level-dependent (BOLD) imaging and intravoxel incoherent motion (IVIM) imaging can be used to predict and diagnose CI-AKI effectively. The value (r > 0.6, < 0.0001) and value (| r| > 0.5, < 0.0001) are strongly correlated with histopathological scores. The NOD-like receptor pyrin 3 (NLRP3) inflammasome participates in CI-AKI and exacerbates CI-AKI in DM rats. Moreover, the percentages of neutrophils and M1 macrophages increase dramatically in rat kidneys after CM injection (neutrophils range from 56.3 to 56.6% and M1 macrophages from 48 to 54.1% in normal rats, whereas neutrophils range from 85.5 to 92.4% and M1 macrophages from 82.1 to 89.8% in DM rats).

Conclusions/interpretation: BOLD and IVIM- can be effective noninvasive tools in predicting CI-AKI. The innate immune response is activated during the progression of CI-AKI and DM will exacerbate this progression.
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http://dx.doi.org/10.3389/fphys.2021.669581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276794PMC
June 2021

Microfluidic Colorimetric Biosensors Based on MnO Nanozymes and Convergence-Divergence Spiral Micromixers for Rapid and Sensitive Detection of .

ACS Sens 2021 08 8;6(8):2883-2892. Epub 2021 Jul 8.

Key Laboratory of Modern Precision Agriculture System Integration Research, Ministry of Education, China Agricultural University, Beijing 100083, China.

In-field screening of foodborne pathogens plays an important role in ensuring food safety. Thus, a microfluidic biosensor was developed for rapid and sensitive detection of using manganese dioxide nanoflowers (MnO NFs) for amplifying the biological signal, a microfluidic chip with a convergence-divergence spiral micromixer for performing automatic operations, and a smartphone app with a saturation calculation algorithm for processing the image. First, immune magnetic nanoparticles (MNPs), the sample, and immune MnO NFs were fully mixed and sufficiently incubated in the spiral micromixer to form MNP-bacteria-MnO sandwich complexes, which were magnetically captured in a separation chamber in the microfluidic chip. Then, a 3,3',5,5'-tetramethylbenzidine (TMB) substrate was injected and catalyzed by a MnO NF nanomimetic enzyme on the complexes, resulting in the production of yellow catalysate. Finally, the catalysate was transferred into a detection chamber and its image was measured and processed using the smartphone app to determine the number of bacteria. This biosensor was able to detect from 4.4 × 10 to 4.4 × 10 CFU/mL in 45 min with a detection limit of 44 CFU/mL, and has the potential to provide a promising platform for on-site detection of foodborne bacteria.
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http://dx.doi.org/10.1021/acssensors.1c00292DOI Listing
August 2021

SAHA could inhibit TGF-β1/p38 pathway in MI-induced cardiac fibrosis through DUSP4 overexpression.

Heart Vessels 2021 Jul 8. Epub 2021 Jul 8.

Department of Cardiology and Institute of Vascular Medicine, Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University Third Hospital, Beijing, 100191, China.

Growing evidences have revealed that a histone deacetylase inhibitor (HDACi), suberoylanilide hydroxamic acid (SAHA) has anti-fibrotic effect in different diseases. In this study, we first evaluated whether SAHA could suppress cardiac fibrosis. Mice with MI-induced cardiac fibrosis were treated with SAHA by intraperitoneal injection and their cardiac function was improved after SAHA treatment. Results of western blotting and qRT-PCR in heart tissues suggested that TGFβ1/P38 pathway was activated in MI mice, and this effect was reversed by SAHA. Cell proliferation assay suggested that SAHA could suppress TGF-β1-induced cardiac fibroblasts proliferation. Furthermore, results of western blotting and qRT-PCR in cardiac fibroblasts depicted that SAHA may exert its anti-fibrotic effect through inhibiting TGF-β1-induced P38 phosphorylation by promoting DUSP4 expression. Our findings may substantiate SAHA as a promising treatment for MI-induced cardiac fibrosis.
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http://dx.doi.org/10.1007/s00380-021-01900-4DOI Listing
July 2021

Determination of Bioelectrical Impedance Thresholds for Early Detection of Breast Cancer-related Lymphedema.

Int J Med Sci 2021 11;18(13):2990-2996. Epub 2021 Jun 11.

Breast Center, Peking University People's Hospital, Beijing, China.

Bioelectrical impedance technology is a common technique used for the early detection of breast cancer-related lymphedema (BCRL). However, studies on the threshold value established by Inbody 720 device (Biospace, Korea) have been extremely limited. We aimed to determine its reference range and cutoff values. All patients were recruited from October 2017 to October 2019 at the Peking University People's Hospital Breast Center. In total, 82 patients with unilateral BCRL and 1305 healthy subjects were recruited in this study. We measured the extracellular fluid (ECF) ratio, extracellular water (ECW) ratio, as well as the single-frequency bioimpedance analysis (SFBIA) ratios at 1 and 5 kHz with the Inbody 720 device. The Youden index-based cutoff points, mean + 2SD and mean + 3SD values of these four indicators for both dominant and nondominant arms were also calculated. Data were collected from 1387 women, including healthy subjects and patients with lymphedema. All statistical analyses were performed with SPSS. Significant differences were found between the two groups in the ECW, ECF, and SFBIA ratios. For the dominant affected arms, the Youden index-based cutoff points for the ECF, ECW, as well as SFBIA ratios at 1 and 5 kHz were 1.009, 1.008, 1.068, and 1.068, respectively. For the nondominant affected arms, the Youden index-based cutoff points were 1.014, 1.013, 1.047, and 1.048, respectively. The mean + 2 standard deviations (SD) and mean + 3SD values were also calculated. We determined the Youden index-based cutoff points, mean + 2SD and mean + 3SD values of the ECF, ECW, as well as SFBIA ratios at 1 and 5 kHz for both dominant and nondominant arms with data from 1305 healthy subjects. Next, the Youden index-based cutoff points, the mean + 2SD and mean + 3SD values were used to recognize patients with lymphedema. We found that the Youden index-based cutoff points and the mean + 2SD showed similar identification capacity on lymphedema, and they seemed to distinguish more patients with lymphedema than mean + 3SD values.
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http://dx.doi.org/10.7150/ijms.53812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241780PMC
June 2021

Machine Learning of Schizophrenia Detection with Structural and Functional Neuroimaging.

Dis Markers 2021 9;2021:9963824. Epub 2021 Jun 9.

Department of Radiology, Xiang'an Hospital of Xiamen University, Xiamen 361002, China.

Schizophrenia (SZ) is a severe psychiatric illness, and it affects around 1% of the general population; however, its reliable diagnosis is challenging. Functional MRI (fMRI) and structural MRI (sMRI) are useful techniques for investigating the functional and structural abnormalities of the human brain, and a growing number of studies have reported that multimodal brain data can improve diagnostic accuracy. Machine learning (ML) is widely used in the diagnosis of neuroscience and neuropsychiatry diseases, and it can obtain high accuracy. However, the conventional ML which concatenated the features into a longer feature vector could not be sufficiently effective to combine different features from different modalities. There are considerable controversies over the use of global signal regression (GSR), and few studies have explored the role of GSR in ML in diagnosing neurological diseases. The current study utilized fMRI and sMRI data to implement a new method named multimodal imaging and multilevel characterization with multiclassifier (M3) to classify SZs and healthy controls (HCs) and investigate the influence of GSR in SZ classification. We found that when we used Brainnetome 246 atlas and without performed GSR, our method obtained a classification accuracy of 83.49%, with a sensitivity of 68.69%, a specificity of 93.75%, and an AUC of 0.8491, respectively. We also got great classification performances with different processing methods (with/without GSR and different brain parcellation schemes). We found that the accuracy and specificity of the models without GSR were higher than that of the models with GSR. Our findings indicate that the M3 method is an effective tool to distinguish SZs from HCs, and it can identify discriminative regions to detect SZ to explore the neural mechanisms underlying SZ. The global signal may contain important neuronal information; it can improve the accuracy and specificity of SZ detection.
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http://dx.doi.org/10.1155/2021/9963824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208855PMC
June 2021

Spatial transcriptome profiling by MERFISH reveals fetal liver hematopoietic stem cell niche architecture.

Cell Discov 2021 Jun 29;7(1):47. Epub 2021 Jun 29.

Department of Genetics, Yale School of Medicine, New Haven, USA.

The hematopoietic stem cell (HSC) niche has been extensively studied in bone marrow, yet a more systematic investigation into the microenvironment regulation of hematopoiesis in fetal liver is necessary. Here we investigate the spatial organization and transcriptional profile of individual cells in both wild type (WT) and Tet2 fetal livers, by multiplexed error robust fluorescence in situ hybridization. We find that specific pairs of fetal liver cell types are preferentially positioned next to each other. Ligand-receptor signaling molecule pairs such as Kitl and Kit are enriched in neighboring cell types. The majority of HSCs are in direct contact with endothelial cells (ECs) in both WT and Tet2 fetal livers. Loss of Tet2 increases the number of HSCs, and upregulates Wnt and Notch signaling genes in the HSC niche. Two subtypes of ECs, arterial ECs and sinusoidal ECs, and other cell types contribute distinct signaling molecules to the HSC niche. Collectively, this study provides a comprehensive picture and bioinformatic foundation for HSC spatial regulation in fetal liver.
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http://dx.doi.org/10.1038/s41421-021-00266-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238952PMC
June 2021

Fe induction strategy for hollow porous N-doped carbon with superior performance in oxygen reduction.

Chem Commun (Camb) 2021 Jul;57(58):7108-7111

College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, P. R. China.

An Fe induction strategy is introduced to achieve template-free synthesis of Co,Fe dual-metal N-codoped hollow porous carbon from zeolitic imidazole frameworks, which is beneficial for the exposure of highly dispersed metal (M)-Nx active sites and enhancement of mass transport, thereby exhibiting a superior electrocatalytic activity (E1/2, 0.86 VRHE).
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http://dx.doi.org/10.1039/d1cc02127cDOI Listing
July 2021

A network analysis investigation of the comorbidity between sleep dysfunction and PTSD symptomatology among Filipino domestic workers in Macao (SAR) China.

J Psychiatr Res 2021 08 30;140:337-345. Epub 2021 May 30.

New York University (Shanghai), People's Republic of China; School of Global Public Health, New York University, New York, NY, USA. Electronic address:

Posttraumatic stress disorder (PTSD) is highly comorbid with sleep dysfunction. This association was previously explained based on cognitive and emotional dysfunction. The current study extends this literature by investigating the symptom level comorbidity of sleep dysfunction and DSM-5 PTSD utilizing a network approach. Participants were trauma-exposed female Filipino domestic workers (N = 1241). Network analysis was applied to 23 items: 18 items from PCL-5 measuring PTSD (Community 1) and 5 items from PSQI assessing sleep dysfunction (Community 2). The results showed that the symptoms within each community had the strongest correlations. Bridge connections were identified between the sleep dysfunction and PTSD symptom communities. Symptoms with the highest bridge strength were concentration difficulties, recklessness, irritability, and sleep disturbance. This is among the first studies investigating the comorbidity between PTSD and sleep dysfunction from the network approach. Future interventions may be developed that emphasize the bridge symptoms to address comorbidity among trauma exposed migrants.
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http://dx.doi.org/10.1016/j.jpsychires.2021.05.040DOI Listing
August 2021

Identification and Validation of Immune-Related lncRNA Signature as a Prognostic Model for Skin Cutaneous Melanoma.

Pharmgenomics Pers Med 2021 3;14:667-681. Epub 2021 Jun 3.

Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.

Purpose: Skin cutaneous melanoma (SKCM) is the most aggressive skin cancer that results in high morbidity and mortality rate worldwide. Immune-related long non-coding RNAs (IRlncRs) play an important role in regulating gene expression in tumors. Therefore, in this study, we aimed to identify IRlncRs signature that could predict prognosis and therapeutic targets for melanoma irrespective of the gene expression levels.

Methods: RNA-sequencing data were obtained from The Cancer Genome Atlas (TCGA). IRlncRs were identified using co-expression analysis and recognized using univariate analysis. The impact of IRlncRs on survival was analyzed using a modified least absolute shrinkage and selection operator (Lasso) regression model. A 1-year survival receiver operating characteristic curve was constructed, and the area under the curve was calculated to identify the optimal cut-off point to distinguish between high and low-risk groups in patients with SKCM. Furthermore, integrative analysis was performed to identify the impact of clinicopathological features, chemotherapeutic treatment, tumor-infiltrating immune cells, and mutant genes on survival.

Results: A total of 28 IRlncRs significantly associated with survival were identified. Seventeen IRlncRs pairs were used to build a survival risk model that could be used to distinguish between low and high-risk groups. The high-risk group was negatively associated with tumor-infiltrating immune cells and had a higher half inhibitory centration for chemotherapeutic agents such as cisplatin and vinblastine. Additionally, the high-risk group had a positive correlation with the expression of specific mutant genes such as BRAF and KIT.

Conclusion: Our findings demonstrate that some IRlncRs have a significant correlation with survival and therapeutic targets for SKCM patients and may provide new insight into the clinical diagnosis and treatment strategies for SKCM patients.
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http://dx.doi.org/10.2147/PGPM.S310299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184246PMC
June 2021

Hypothyroidism is associated with clinical outcomes in patients with acute myocardial infarction: subgroup analysis of China PEACE study.

Endocrine 2021 Oct 3;74(1):128-137. Epub 2021 Jun 3.

Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China.

Purpose: Thyroid dysfunction contributes to adverse events in several types of cardiovascular diseases. The aim of the present study is to determine whether thyroid status is associated with the prognosis of patients with acute myocardial infarction (AMI).

Methods: The present cohort arose from the China PEACE‑Prospective AMI study. Based on the evaluation of thyroid status, participants were divided into euthyroid, hypothyroid, and hyperthyroid groups. A total of 2569 AMI patients met the inclusion criteria of our present study. The primary outcomes were the 12-month composite cardiovascular endpoint (CCVE, a composite of all-cause death, myocardial infarction, revascularization, and heart failure) and the composite cardio-cerebral vascular endpoint (CCCVE, comprising CCVE and stroke).

Results: Of the entire cohort, 431 patients (16.8%) confirmed hypothyroid status and 102 (4.0%) were at hyperthyroid status. There were total 594 CCVEs (23.1%) and 687 CCCVEs (26.7%) in the general population. After adjusting conventional risk factors, AMI patients from the hypothyroid status group were at increased risk of the two composite endpoints, compared with euthyroid individuals (CCVE, HR:1.337, 95%CI: 1.097-1.630; CCCVE, HR:1.336, 95%CI: 1.111-1.607). However, no significant increased trends of the two composite endpoints could be observed in hyperthyroid group. Furthermore, hypothyroid status was also independently associated with a higher risk of revascularization (HR: 1.648, 95%CI: 1.047-2.595) and heart failure (HR: 1.382, 95%CI: 1.066-1.792).

Conclusion: Compared with euthyroid status, hypothyroid status has an independent predicting value for adverse cardiovascular events in AMI patients. Further investigations are required to illustrate whether treatment of thyroid dysfunction could improve the prognosis of AMI patients.
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http://dx.doi.org/10.1007/s12020-021-02742-wDOI Listing
October 2021

A matter of perspective-Cutting-edge technology-driven urine proteome in COVID-19.

Urine (Amst) 2021 27;3:1-2. Epub 2021 May 27.

Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University, Beijing, 100191, China.

In a recent issue of Nature Communications, we highlighted in-depth urine proteomic research in which significant immunosuppression was revealed in early SARS-CoV-2- infected patients 1. The application of urine in mapping the landscape of molecular changes closely associated with human diseases has been widely accepted. Herein, we take a systematic review of the published article from the perspective of both methodology and clinical significance.
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http://dx.doi.org/10.1016/j.urine.2021.01.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157115PMC
May 2021

Application of Functional Magnetic Resonance Imaging in the Diagnosis of Parkinson's Disease: A Histogram Analysis.

Front Aging Neurosci 2021 11;13:624731. Epub 2021 May 11.

Department of Radiology, Xiang'an Hospital of Xia Men University, Xiamen, China.

This study aimed to investigate the value of amplitude of low-frequency fluctuation (ALFF)-based histogram analysis in the diagnosis of Parkinson's disease (PD) and to investigate the regions of the most important discriminative features and their contribution to classification discrimination. Patients with PD ( = 59) and healthy controls (HCs; = 41) were identified and divided into a primary set (80 cases, including 48 patients with PD and 32 HCs) and a validation set (20 cases, including 11 patients with PD and nine HCs). The Automated Anatomical Labeling (AAL) 116 atlas was used to extract the histogram features of the regions of interest in the brain. Machine learning methods were used in the primary set for data dimensionality reduction, feature selection, model construction, and model performance evaluation. The model performance was further validated in the validation set. After feature data dimension reduction and feature selection, 23 of a total of 1,276 features were entered in the model. The brain regions of the selected features included the frontal, temporal, parietal, occipital, and limbic lobes, as well as the cerebellum and the thalamus. In the primary set, the area under the curve (AUC) of the model was 0.974, the sensitivity was 93.8%, the specificity was 90.6%, and the accuracy was 93.8%. In the validation set, the AUC, sensitivity, specificity, and accuracy were 0.980, 90.9%, 88.9%, and 90.0%, respectively. ALFF-based histogram analysis can be used to classify patients with PD and HCs and to effectively identify abnormal brain function regions in PD patients.
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http://dx.doi.org/10.3389/fnagi.2021.624731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144304PMC
May 2021

Diversity and distribution of viruses inhabiting the deepest ocean on Earth.

ISME J 2021 10 10;15(10):3094-3110. Epub 2021 May 10.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

As the most abundant biological entities on the planet, viruses significantly influence the overall functioning of marine ecosystems. The abundance, distribution, and biodiversity of viral communities in the upper ocean have been relatively well studied, but our understanding of viruses in the hadal biosphere remains poor. Here, we established the oceanic trench viral genome dataset (OTVGD) by analysing 19 microbial metagenomes derived from seawater and sediment samples of the Mariana, Yap, and Kermadec Trenches. The trench viral communities harbored remarkably high novelty, and they were predicted to infect ecologically important microbial clades, including Thaumarchaeota and Oleibacter. Significant inter-trench and intra-trench exchange of viral communities was proposed. Moreover, viral communities in different habitats (seawater/sediment and depth-stratified ocean zones) exhibited distinct niche-dependent distribution patterns and genomic properties. Notably, microbes and viruses in the hadopelagic seawater seemed to preferably adopt lysogenic lifestyles compared to those in the upper ocean. Furthermore, niche-specific auxiliary metabolic genes were identified in the hadal viral genomes, and a novel viral D-amino acid oxidase was functionally and phylogenetically characterized, suggesting the contribution of these genes in the utilization of refractory organic matter. Together, these findings highlight the genomic novelty, dynamic movement, and environment-driven diversification of viral communities in oceanic trenches, and suggest that viruses may influence the hadal ecosystem by reprogramming the metabolism of their hosts and modulating the community of keystone microbes.
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http://dx.doi.org/10.1038/s41396-021-00994-yDOI Listing
October 2021

Elaboration of Non-naturally Occurring Helical Tripeptides as p53-MDM2/MDMX Interaction Inhibitors.

Chem Pharm Bull (Tokyo) 2021 Jul 29;69(7):681-692. Epub 2021 Apr 29.

Graduate School of Pharmaceutical Sciences, The University of Tokyo.

Protein-protein interactions (PPIs) are often mediated by helical, strand and/or coil secondary structures at the interface regions. We previously showed that non-naturally occurring, stable helical trimers of bicyclic β-amino acids (Abh) with all-trans amide bonds can block the p53-MDM2/MDMX α-helix-helix interaction, which plays a role in regulating p53 function. Here, we conducted docking and molecular dynamics calculations to guide the structural optimization of our reported compounds, focusing on modifications of the C-terminal/N-terminal residues. We confirmed that the modified peptides directly bind to MDM2 by means of thermal shift assay, isothermal titration calorimetry, and enzyme-linked immunosorbent assay (ELISA) experiments. Biological activity assay in human osteosarcoma cell line SJSA-1, which has wild-type p53 and amplification of the Mdm2 gene, indicated that these peptides are membrane-permeable p53-MDM2/MDMX interaction antagonists that can rescue p53 function in the cells.
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http://dx.doi.org/10.1248/cpb.c21-00238DOI Listing
July 2021

A Clinical Study of Minilaparoscopy in the Treatment of Cryptorchidism with an Ipsilateral Inguinal Hernia.

J Laparoendosc Adv Surg Tech A 2021 May 4. Epub 2021 May 4.

Department of Hernia and Abdominal Wall Surgery, Capital Medical University, Beijing Chaoyang Hospital, Beijing, P. R. China.

The aim of this study was to investigate the safety and efficacy of performing minilaparoscopy compared with standard laparoscopy in the treatment of cryptorchidism with an ipsilateral inguinal hernia. In total, 46 patients with cryptorchidism and an ipsilateral inguinal hernia were admitted to the Urology and Hernia and Abdominal Wall Surgery Departments of Beijing Chaoyang Hospital between October 2009 and July 2019. They were assigned to two groups: Group M and Group S. In Group M, 24 patients underwent herniorrhaphy and orchiopexy using minilaparoscopy, and in Group S, 22 patients underwent herniorrhaphy and orchiopexy using standard laparoscopy. Surgeons chose the procedure at random, and the patients were blinded to the selected procedure. Postoperative painkiller demand ( = .043) and first postoperative day Numerical Rating Scale scores ( = .032) were lower in Group M than Group S, and the average hospital stay was shorter ( = .041) in Group M. Furthermore, 21 of the 24 procedures in Group M were successful, 3 procedures of Group M were converted from mini- to standard laparoscopy, and all 22 procedures in Group S were successful. The Observer Scar Assessment Scale questionnaire results of Group M were significantly higher than for patients in Group S ( = .038). Our findings suggest that treatment of cryptorchidism with ipsilateral inguinal hernia using minilaparoscopy is as safe and effective as standard laparoscopy.
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http://dx.doi.org/10.1089/lap.2020.1038DOI Listing
May 2021

Chromatin tracing and multiplexed imaging of nucleome architectures (MINA) and RNAs in single mammalian cells and tissue.

Nat Protoc 2021 05 26;16(5):2667-2697. Epub 2021 Apr 26.

Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.

The genome is hierarchically organized into several 3D architectures, including chromatin loops, domains, compartments and regions associated with nuclear lamina and nucleoli. Changes in these architectures have been associated with normal development, aging and a wide range of diseases. Despite its critical importance, understanding how the genome is spatially organized in single cells, how organization varies in different cell types in mammalian tissue and how organization affects gene expression remains a major challenge. Previous approaches have been limited by a lack of capacity to directly trace chromatin folding in 3D and to simultaneously measure genomic organization in relation to other nuclear components and gene expression in the same single cells. We have developed an image-based 3D genomics technique termed 'chromatin tracing', which enables direct 3D tracing of chromatin folding along individual chromosomes in single cells. More recently, we also developed multiplexed imaging of nucleome architectures (MINA), which enables simultaneous measurements of multiscale chromatin folding, associations of genomic regions with nuclear lamina and nucleoli and copy numbers of numerous RNA species in the same single cells in mammalian tissue. Here, we provide detailed protocols for chromatin tracing in cell lines and MINA in mammalian tissue, which take 3-4 d for experimental work and 2-3 d for data analysis. We expect these developments to be broadly applicable and to affect many lines of research on 3D genomics by depicting multiscale genomic architectures associated with gene expression, in different types of cells and tissue undergoing different biological processes.
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http://dx.doi.org/10.1038/s41596-021-00518-0DOI Listing
May 2021

Design, synthesis and biological evaluation of selective histone deacetylase 6 (HDAC6) inhibitors bearing benzoindazole or pyrazoloindazole scaffold as surface recognition motif.

Bioorg Chem 2021 06 20;111:104910. Epub 2021 Apr 20.

Key Laboratory of Structure-Based Drugs Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address:

A series of compounds were designed and synthesized based on the compound 11i bearing phenylpyrazole scaffold with histone deacetylase 6 (HDAC6) inhibitory activity. Most of the compounds showed considerable inhibitory activity against HDAC6 and compound A16 with good inhibitory activity was found therein. We further found that A16 had an inhibitory effect on inflammatory mediators (NO, TNF-α, IL-6) involved in inflammatory response and neuroendocrine regulation. In addition, A16 has a certain neuroprotective effect on PC12 cells injured by hydrogen peroxide. Acute toxicity assay showed that the LD of A16 was 274.47 mg/kg in mouse model. Furthermore, A16 displayed good stability properties in microsomes and plasma.
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http://dx.doi.org/10.1016/j.bioorg.2021.104910DOI Listing
June 2021

Application of the ACOSOG Z0011 criteria to Chinese patients with breast cancer: a prospective study.

World J Surg Oncol 2021 Apr 20;19(1):128. Epub 2021 Apr 20.

Breast Center, Peking University People's Hospital, Beijing, China.

Background: Although the ACOSOG Z0011 study showed that axillary lymph node dissection (ALND) could be avoided in a specific population of sentinel lymph node-positive patients, it is not widely accepted by Chinese surgeons. We conducted a prospective single-arm study to confirm whether or not the results of Z0011 are applicable to Chinese patients.

Methods: Patients conforming to the Z0011 criteria were prospectively enrolled at the Peking University People's Hospital Breast Center from November 2014 to June 2019. The clinicopathological features of the study group were compared with those of the Z0011 study group. Lymphedema after surgery, the incidence of local-regional recurrence, and survival were analyzed.

Results: One hundred forty-two patients who met the Z0011 eligibility criteria were enrolled in this study; 115 underwent sentinel lymph node biopsy (SLNB) alone. Compared with the Z0011 trial, younger patients were included (median age, 52 [26-82] years vs 54 [25-90] years; P = 0.03). For clinical T stage, tumor histology, hormone status, lymphovascular invasion, and the number of positive sentinel lymph nodes (SLNs), no statistically significant differences were observed. More patients received adjuvant chemotherapy and endocrine therapy in this study (90.85% vs 58.0% and 80.99% vs 46.6% respectively, P <0.001). A similar percentage of patients received radiotherapy, but more nodal radiotherapy procedures were carried out in our study (54.5% vs 16.9%). After a median follow-up of 29 months, only 1 patient (0.9%) had ipsilateral breast tumor recurrence, and no regional recurrence occurred.

Conclusion: Our study showed that it is achievable to avoid ALND in patients eligible for Z0011 in China.

Trial Registration: ClinicalTrials.gov. Registration number NCT03606616 . Retrospectively registered on 31 July 2018.
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http://dx.doi.org/10.1186/s12957-021-02242-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059271PMC
April 2021

Biochar heavy metal removal in aqueous solution depends on feedstock type and pyrolysis purging gas.

Environ Pollut 2021 Jul 7;281:117094. Epub 2021 Apr 7.

Department of Renewable Resources, University of Alberta, Edmonton, Alberta, T6G 2E3, Canada. Electronic address:

The effectiveness of biochar as a sorptive material to remove contaminants, particularly heavy metals, from water is dependent on biomass type and pyrolysis condition. Biochars were produced from pulp mill sludge (PMS) and rice straw (RS) with nitrogen (N) or carbon dioxide (CO) as the purging gas. The sorptive capacity of the biochars for cadmium(II), copper(II), nickel(II) and lead(II) was studied. The heavy metal adsorption capacity was mainly affected by biomass type, with biochars adsorption capacities higher for lead(II) (109.9-256.4 mg g) than for nickel(II) (40.2-64.1 mg g), cadmium(II) (29.5-42.7 mg g) and copper(II) (18.5-39.4 mg g) based on the Langmuir adsorption model. The highest lead(II) adsorption capacities for PMS and RS biochars were 256.4 and 133.3 mg g, respectively, when generated using N as the purging gas. The corresponding lead(II) adsorption capacities were 250.0 and 109.9 mg g, respectively, when generated using CO as the purging gas. According to the intraparticle diffusion model, 30-62% of heavy metal adsorption was achieved in 1 h; film diffusion was the rate-dominating step, whereas pore diffusion was a rate-limiting step. Ion exchange and complexation between heavy metals and biochar surface functional groups such as carbonyl and hydroxyl groups were effective mechanisms for heavy metal sorption from the aqueous solution. We conclude that proper selection of both the feedstock type and the purging gas is important in designing biochars for the effective removal of potentially toxic metals from wastewater.
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http://dx.doi.org/10.1016/j.envpol.2021.117094DOI Listing
July 2021

Competitive activation cross amplification combined with smartphone-based quantification for point-of-care detection of single nucleotide polymorphism.

Biosens Bioelectron 2021 Jul 26;183:113200. Epub 2021 Mar 26.

National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, Key Laboratory of Zoonoses, Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong Province, Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

In this study, we firstly propose a novel smartphone-assisted visualization SNP genotyping method termed competitive activation cross amplification (CACA). The mutation detection strategy depends on the ingenious design of both a start primer and a verification probe with ribonucleotide insertion through competitive combination and perfect matching with the target DNA, Meanwhile, the RNase H2 enzyme was utilized to specifically cleave ribonucleotide insertion and achieve extremely specific dual verification. Simultaneously, the results allow both colorimetric and fluorescence product dual-mode visualization by using self-designed 3D-printed dual function cassette. We validated this novel CACA by analyzing the Salmonella Pullorum rfbS gene at the 237th site, successfully solve the current bottleneck of specific identification and visual detection of this pathogen. The concentration detection limits of the plasmid and genomic DNA were 1500 copies/μL and 3.98 pg/μL, respectively, and as low as the presence of 0.1% mutant-type can be distinguished from 99.9% wild-type. Combined with a powerful hand-warmer, which can provide heating more than 60 °C for 20 h to realize power-free, dual function cassette and smartphone quantitation, our novel CACA platform firstly realizes user-friendly, cost-effective, portable, rapid, and accurate POC detection of SNP.
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http://dx.doi.org/10.1016/j.bios.2021.113200DOI Listing
July 2021

CrI/YCH Heterointerface-Induced Stable Half-Metallicity of Two-Dimensional CrI Monolayer Ferromagnets.

ACS Appl Mater Interfaces 2021 Apr 5;13(14):16694-16703. Epub 2021 Apr 5.

Institute of Fundamental and Frontier Sciences & School of Mechanical and Electrical Engineering, University of Electronic Science and Technology of China, Chengdu 610054, China.

Two-dimensional (2D) CrI monolayer ferromagnets are key to the development of future miniature spintronic devices and modulating them into a half-metal will greatly expand the application scenarios of CrI in nanospintronics. Nevertheless, existing strategies to induce half-metallicity of a CrI monolayer remain experimentally challenging and have unstable issues. In this work, the introduction of a 2D electride [YC]·2e as an auxiliary layer is shown to be an effective way to achieve the generation of stable half-metallicity in the CrI monolayer. When the fully hydrogenated YCH and ferromagnetic CrI monolayer combine to form a heterostructure, surprisingly the appropriate amount of charge injection (0.72 ) turns CrI into a half-metal. Hetero-interfacial half-metallicity in CrI is an intrinsic one and does not require any chemical functionalization or external physical modification. Therefore, it is advantageous for practical applications of CrI in miniature spintronic devices, such as magnetic tunnel junctions, spin valves or spin field-effect transistors. A new strategy of the stable CrI/YCH heterostructure was successfully developed to induce the half-metallicity of 2D CrI ferromagnets, which is experimentally feasible and half-metallic stable enough. This work paves the way for the application of the CrI monolayer in half-metallic-based spintronics.
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http://dx.doi.org/10.1021/acsami.1c01768DOI Listing
April 2021

Assessment of CPS + EG, Neo-Bioscore and Modified Neo-Bioscore in Breast Cancer Patients Treated With Preoperative Systemic Therapy: A Multicenter Cohort Study.

Front Oncol 2021 16;11:606477. Epub 2021 Mar 16.

Institute of Mental Health, Peking University, Beijing, China.

This study was to assess the prognosis stratification of the clinical-pathologic staging system incorporating estrogen receptor (ER)-negative disease, the nuclear grade 3 tumor pathology (CPS + EG), Neo-Bioscore, and a modified Neo-Bioscore system in breast cancer patients after preoperative systemic therapy (PST). A retrospective multicenter cohort study was conducted from 12 participating hospitals' databases from 2006 to 2015. Five-year disease free survival (DFS), disease specific survival (DSS), and overall survival (OS) were calculated using Kaplan-Meier Method. Area under the curve (AUC) of the three staging systems was compared. Wald test and maximum likelihood estimates in Cox proportional hazards model were used for multivariate analysis. A total of 1,077 patients were enrolled. The CPS + EG, Neo-Bioscore, and modified Neo-Bioscore could all stratify the DFS, DSS, and OS (all P < 0.001). While in the same stratum of Neo-Bioscore scores 2 and 3, the HER2-positive patients without trastuzumab therapy had much poorer DSS (P = 0.013 and P values < 0.01, respectively) as compared to HER2-positive patients with trastuzumab therapy and HER2-negative patients. Only the modified Neo-Bioscore had a significantly higher stratification of 5-year DSS than PS (AUC 0.79 . 0.65, P = 0.03). So, the modified Neo-Bioscore could circumvent the limitation of CPS + EG or Neo-Bioscore.

Clinical Trial Registration: ClinicalTrials.gov, identifier NCT03437837.
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http://dx.doi.org/10.3389/fonc.2021.606477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009183PMC
March 2021

Evaluating and Balancing the Risk of Breast Cancer-Specific Death and Other Cause-Specific Death in Elderly Breast Cancer Patients.

Front Oncol 2021 12;11:578880. Epub 2021 Mar 12.

Breast Center, Peking University People's Hospital, Beijing, China.

The dilemma of undertreatment and overtreatment of elderly breast cancer patients is common. This study aimed to investigate clinicopathological features, treatment modalities, and survival in women diagnosed with breast cancer at age 70 years or over, and to assist clinicians in developing individualized treatment plans by balancing the risks of breast cancer-specific death (BCSD) and other cause-specific death (OCSD). This retrospective study included 420 women who were diagnosed with pathologically confirmed invasive breast cancer at age 70 years or older from January 2008 to December 2015 at Peking University People's Hospital (PKUPH). We collected baseline health status, tumor characteristics, treatment choices, and outcomes and created nomograms for clinicians to estimate individualized BCSD and OCSD risk directly. During a median follow-up of 71.5 months (range 2 to 144 months) in patients with stage I-III tumors, breast cancer specific survival (BCSS) was 92.4% (376/407) and overall survival (OS) was 78.1% (318/407). There were 89 deaths, and 65.2% (58/89) were non-breast cancer related. Upon multivariate analysis by Cox regression model, tumor size, positive lymph nodes, Ki-67, and surgery were independent predictors of BCSS, and comorbidities, positive lymph nodes, Ki-67, surgery, and endocrine therapy were independent predictors of OS. Propensity score weighted (PSW) was applied to analyze therapeutic efficacy, and there was BCSS and OS benefit with surgery (both < 0.001), BCSS benefit with chemotherapy ( = 0.029), BCSS and OS benefit with endocrine therapy ( = 0.006 and 0.004), and neither BCSS nor OS benefit with radiotherapy (RT) ( = 0.348 and 0.289). Competing-risk nomograms were developed to estimate cumulative mortality probabilities for BCSD and OCSD for individual patients according to clinicopathologic characteristics and treatments. The calibration curves displayed exceptionally, with C-indexes 0.714 for BCSD and 0.717 for OCSD. Older patients had greater risk of dying from non-breast cancer causes. Surgery, chemotherapy, and endocrine therapy were associated with improved survival. Competing risk nomograms allowed individual assessment of BCSD and OCSD, based on clinicopathological characteristics and treatment options, and can be used as a tool to help in choosing appropriate treatment strategies. This study was approved by the Peking University People's Hospital Research Ethics Board on September 4, 2018.
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http://dx.doi.org/10.3389/fonc.2021.578880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994517PMC
March 2021

Long-term follow-up results of fluorescence and blue dye guided sentinel lymph node biopsy in early breast cancer.

Breast Cancer Res Treat 2021 Jul 24;188(2):361-368. Epub 2021 Mar 24.

Breast Center, Peking University People's Hospital, Beijing, 100044, People's Republic of China.

Purpose: This study aimed to assess the efficacy of the combination of indocyanine green (ICG) and methylene blue (MB) dye in early breast cancer patients undergoing sentinel lymph node biopsy (SLNB).

Methods: Between January 2011 and December 2015, 1061 early breast cancer patients underwent SLNB were included. SLNB was performed for enrolled patients by injection of both ICG and MB. Detection rate, positivity, and number of sentinel lymph nodes (SLNs) by ICG and MB were analysed. Axillary recurrence and arm lymphedema at 5.6-year follow-up were reported.

Results: The combination of ICG and MB resulted in a very high detection rate of 99.6%, the median number of sentinel lymph nodes was 3. A total of 374 histologically confirmed positive SLNs were detected in 237 patients, 96.6% of the positive patients and 94.1% of the positive nodes could be identified by the combination of ICG and MB. After a median follow-up of 5.6 (2-9.3) years, 0.64% of patients with negative SLNs had ipsilateral axillary recurrence, and the incidence of arm lymphedema was 2.1%.

Conclusions: Although the missing isotope control weakens the interpretation of the findings, the dual tracing modality of ICG and MB, without involvement of radioactive isotopes, was an effective method for SLNB in patients with early breast cancer.

Trial Registration: ACTRN12612000109808. Registered on 23 January 2012.
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http://dx.doi.org/10.1007/s10549-021-06196-6DOI Listing
July 2021

Identification of Subclinical Myocardial Dysfunction in Breast Cancer Patients with Metabolic Syndrome after Cancer-Related Comprehensive Therapy.

Cardiol Res Pract 2021 2;2021:6640673. Epub 2021 Mar 2.

Department of Cardiology, Peking University People's Hospital, Beijing, China.

Background: Breast cancer patients with metabolic syndrome have an increased risk of cardiovascular disease. These patients are more prone to suffer from cardiotoxicity after anticancer therapy. Patients after completion of cancer-related comprehensive therapy, who show normal myocardial function, may already have subclinical myocardial dysfunction. We sought to evaluate the subclinical myocardial dysfunction in breast cancer patients with metabolic syndrome after cancer-related comprehensive therapy. Methods. In this study, 45 breast cancer patients with metabolic syndrome after completion of cancer-related comprehensive therapy, 45 non-breast cancer patients with metabolic syndrome, and 30 breast cancer patients without metabolic syndrome after therapy were enrolled. Left ventricular ejection fraction (LVEF) and global longitudinal strain (GLS) were measured using echocardiogram.

Results: All the patients had normal LVEF. However, nine breast cancer patients with metabolic syndrome (20%) had GLS that was lower than -17%, while all the noncancer patients had normal GLS. Breast cancer patients with metabolic syndrome had a decrease of GLS and LVEF, compared with noncancer patients with metabolic syndrome. Furthermore, we found that decrease of age was associated with reduction of LVEF and that use of trastuzumab for 1 year was a significant factor associated with reduction of GLS. In addition, breast cancer patients with metabolic syndrome had a decrease of GLS, compared with breast cancer patients without metabolic syndrome after cancer-related therapy.

Conclusions: Breast cancer patients with metabolic syndrome after completion of cancer-related comprehensive therapy suffered from subclinical myocardial dysfunction. GLS should be routinely performed to early identify subclinical myocardial damage of patients, in order to prevent the cardiotoxicity of cancer-related comprehensive therapy.
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http://dx.doi.org/10.1155/2021/6640673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943305PMC
March 2021
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