Publications by authors named "Siyi Liu"

43 Publications

Corrigendum to "Ovarian metastasis risk factors in endometrial carcinoma: A systematic review and meta-analysis" [Eur J Obstet Gynecol Reprod Biol 267 (2021) 245-255].

Eur J Obstet Gynecol Reprod Biol 2021 Dec 29;269:83. Epub 2021 Dec 29.

Department of Gynecology, Guangxi Medical University First Affiliated Hospital, Nanning, Guangxi, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejogrb.2021.12.005DOI Listing
December 2021

Ovarian metastasis risk factors in endometrial carcinoma: A systematic review and meta-analysis.

Eur J Obstet Gynecol Reprod Biol 2021 Dec 13;267:245-255. Epub 2021 Nov 13.

Department of Gynecology, Guangxi Medical University First Affiliated Hospital, Nanning, Guangxi, China. Electronic address:

Objective: To investigate the incidence of ovarian metastasis in endometrial carcinoma (EC) and analyze its risk factors and provide a theoretical basis for whether retention of the ovary in patients with EC.

Methods: A systematic search using synonyms of 'ovarian cancer' and 'metastasis' was conducted in PubMed, Cochrane database, Embase, Google Scholar, and WOS database. Meta-analysis was performed on 7 included studies, comprising 4281 clinical-stage I-IV EC patients. Studies were assessed using the Newcastle-Ottawa Scale (NOS) criteria. Odds risks (OR) and 95% confidence intervals (CI) were calculated using an inverse variance weighted random-effects model.

Results: The ovarian metastasis risk of EC was significantly higher for patients with myometrial invasion >1/2 (OR = 18.19, 95% CI 5.34 to 61.96 compared to myometrial invasion ≤1/2), any pelvic lymph node invasion (PLNI) (OR = 5.41, 95% CI 2.60-10.97 compared to without PLNI), G3 pathological grade (OR = 2.66, 95%CI 1.35-5.24 compared to G1-G2), non-endometrioid pathological type (OR = 6.46, 95% CI 3.25 to 12.83 compared to endometrioid), lymphatic vascular space invasion (LVSI) (OR = 6.46, 95% CI 3.25 to 12.83 compare to without LVSI), age >45 (OR = 2.01, 95% CI 0.29 to 14.11 compared to age ≤45), and cervical invasion (OR = 4.12, 95% CI 1.87 to 9.08 compared to without cervical invasion).

Conclusion: About 4.95% of EC patients develop ovarian metastasis. Age >45, myometrial invasion >1/2, cervical invasion, PLNI, pathological type, G3 pathological grade, and LVSI were the high-risk factors for ovarian metastasis of EC. Ovarian preservation should be carefully selected for patients with EC, and preoperative and intraoperative evaluations should be entirely performed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejogrb.2021.11.016DOI Listing
December 2021

Pathogenic Characteristics and Risk Factors for ESKAPE Pathogens Infection in Burn Patients.

Infect Drug Resist 2021 12;14:4727-4738. Epub 2021 Nov 12.

Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, People's Republic of China.

Objective: This study aimed to determine the clinical manifestations, antimicrobial resistance, molecular characteristics, and risk factors for ESKAPE pathogens infection in burn patients.

Methods: A retrospective study of 187 burn patients infected with ESKAPE pathogens was conducted at the Department of Plastic and Burn Surgery of the Affiliated Hospital of Southwest Medical University (Luzhou, China) from October 2018 to June 2021. All strains were identified using a MicroScan WalkAway 96 Plus System, and antimicrobial susceptibilities were determined using the VITEK system or the disk diffusion method. The antimicrobial resistance genes of multi-drug resistant ESKAPE (MDR-ESKAPE) were detected by polymerase chain reaction (PCR). The multivariable logistic regression analysis was used to estimate the risk factors for ESKAPE infection and MDR-ESKAPE infection.

Results: A total of 255 strains were isolated in various types of clinical specimens from 187 burn patients, of which 47.5% were ESKAPE pathogens (121/255). Among these, MDR-ESKAPE pathogens accounted for 55% (67/121). Additionally, , , , and were the most prevalent genes detected in , and spp., respectively. The independent risk factors for ESKAPE infection were total body surface area (TBSA) >30-50% (odds ratio [OR] = 10.428; 95% confidence interval [CI], 2.047 to 53.108), TBSA >50% (OR = 15.534; 95% CI, 1.489 to 162.021), and parenteral nutrition (OR = 3.597; 95% CI, 1.098 to 11.787). No independent risk factors were found for MDR-ESKAPE infection.

Conclusion: Clinical staff should be alert to the risk of nosocomial infection with ESKAPE pathogens in burn patients receiving parenteral nutrition and under TBSA >30%. Full attention should also be paid to the ESKAPE resistance, strict adherence to infection control protocols for the rational use of antimicrobial agents, and enhanced clinical standardization of antimicrobial agents management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IDR.S338627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594746PMC
November 2021

Resting-state functional connectivity within orbitofrontal cortex and inferior frontal gyrus modulates the relationship between reflection level and risk-taking behavior in internet gaming disorder.

Brain Res Bull 2022 Jan 30;178:49-56. Epub 2021 Oct 30.

School of Psychology and Cognitive Science, East China Normal University, Shanghai, PR China; Shanghai Key Laboratory of Magnetic Resonance, School of Physics and Materials Science, East China Normal University, Shanghai, PR China.

Internet gaming disorder (IGD) has become an increasing mental health issue worldwide. Previous studies indicated that IGD was related to maladaptive risk-taking behavior. However, the relationship among risk-taking behavior, reflection level, and resting-state functional connectivity (rsFC) between brain regions in IGD individuals remains unclear. The current study combined resting-state fMRI and the Devil task to investigate this issue. The behavioral results suggested that IGD participants exhibited increased risk-taking behavior in the Devil task than healthy controls. Moreover, IGD participants' risk-taking behavior was positively correlated with their reflection level. As for fMRI results, IGD participants showed stronger rsFC between orbitofrontal cortex (OFC) and inferior frontal gyrus (IFG) than healthy controls. Additionally, the mediation analyses revealed that, among IGD participants, the rsFC between OFC and IFG fully mediated the relationship between reflection level and risk-taking behavior. Together, the current study highlighted that the altered rsFC between OFC and IFG in IGD individuals modified the relationship between their reflection level and risk-taking behavior, which might contribute to the understanding of neural mechanisms underlying risk-taking behavior in IGD individuals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.brainresbull.2021.10.019DOI Listing
January 2022

Evaluation of the Stress Tolerance of with Different Antibiotic Resistance Profiles.

Biomed Res Int 2021 14;2021:5604458. Epub 2021 Sep 14.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, MOA Laboratory of Quality & Safety Risk Assessment for Agro-Products (Hangzhou), Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

Disease caused by antibiotic-resistant is a serious clinical problem that poses a great threat to public health. The present study is aimed at assessing differences in bacterial kinetics with different antibiotic resistance profiles under environmental stress and at developing microbial tolerance models in lettuce during storage from 4 to 36°C. The drug-resistance phenotypes of 10 Typhimurium (. Typhimurium) isolates were examined using the broth microdilution method. The results of 10 S. Typhimurium isolates in the suspensions showed that a slow trend towards reduction of drug-sensitive (DS) isolates in relation to the others though without statistical difference. Compared to DS . Typhimurium SA62, greater bacterial reduction was observed in multidrug-resistant (MDR) . Typhimurium HZC3 during lettuce storage at 4°C ( < 0.05). It was likely that a cross-response between antibiotic resistance and food-associated stress tolerance. The greater growth in lettuce at 12°C was observed for DS . Typhimurium SA62 compared to MDR . Typhimurium HZC3 and was even statistically different ( < 0.05), while no significant difference was observed for bacterial growth between MDR . Typhimurium HZC3 and DS . Typhimurium SA62 strains in lettuce storage from 16 to 36°C ( > 0.05). The goodness-of-fit indices indicated the Log-linear primary model provided a satisfactory fit to describe the MDR . Typhimurium HZC3 and DS . Typhimurium SA62 survival at 4°C. A square root secondary model could be used to describe the effect of temperature (12, 16, 28, and 36°C) on the growth rates of . Typhimurium HZC3 ( - = 0.91, RMSE = 0.06) and . Typhimurium SA62 ( - = 0.99, RMSE = 0.01) derived from the Huang primary model. It was necessary to pay attention to the tolerance of antibiotic resistant bacteria under environmental stress, and the generated models could provide parts of the input data for microbial risk assessment of with different antibiotic resistance profile in lettuce.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5604458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457946PMC
January 2022

The complete mitochondrial genome of (Hymenoptera: Cimbicidae) and phylogenetic analysis.

Mitochondrial DNA B Resour 2021 Jun 21;6(7):2031-2032. Epub 2021 Jun 21.

Jiangxi Normal University, Nanchang, PR China.

The complete mitochondrial genome of was sequenced with 15,127 bp in length. The mitogenome includes 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs genes, and an AT-rich region. The nucleotide compositions of (43.7% A, 38.0% T, 7.4% G, and 10.9% C) were biased toward A and T. Based on Bayesian inference (BI) and Maximum Likelihood (ML) analyses, was identified as one of the basal lineages of family Cimbicidae.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1907800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218853PMC
June 2021

Platelet-to-Lymphocyte Ratio is Associated with the Mortality in Peritoneal Dialysis Patients.

Iran J Kidney Dis 2021 May;15(3):206-212

Department of Nephrology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China.

Introduction: Platelet-to-lymphocyte ratio (PLR) is widely used as an inflammatory marker and is associated with poor prognosis in some diseases, such as cardiovascular diseases and malignancies. However, the association between the PLR and all-cause mortality in peritoneal dialysis (PD) patients is unclear.

Methods: A total of 939 patients were enrolled. The X-tile program was performed to calculate the optimal cut-off values for the PLR, and the patients were divided into three groups according to the cut-off values: a low PLR group (< 108.33), medium PLR group (108.33 to 257.50), and high PLR group (> 257.50). Multivariate analysis was performed to assess the prognostic value of PLR. The primary end point was all-cause mortality.

Results: Of the 939 patients, the mean age was 49.9 years, and 57% of the patients were male. During a median follow-up of 27.5 months (interquartile range, 13.6-41.6 months), 221 (23.5%) died, in whom 114 (51.6%) deaths were attributed to cardiovascular mortality. Patients in the high PLR group had a higher mortality rate than patients in the low PLR group (log rank = 13.75, P < .001). The 1-year and 3-year overall survival rates were 88.9% and 71.7% for patients in the high PLR group compared with 98.6% and 86.2% for patients in the low PLR group, respectively. Similarly, multivariate Cox regression analysis showed that the mortality rate was higher in the high PLR group than in the low PLR group (HR = 1.64, 95% CI: 1.02 to 2.63, P < .05).

Conclusion: An increased PLR value was independently associated with all-cause mortality in PD patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
May 2021

The modulation of attentional deployment on regret: an event-related potential study.

Neuroreport 2021 05;32(7):621-630

Shaanxi Key Laboratory of Behavior and Cognitive Neuroscience, School of Psychology, Shaanxi Normal University, Xi'an.

Adopting a sequential risk-taking task, this study explored the modulation of attentional deployment on regret. Attentional deployment was manipulated during outcome feedback of the task by highlighting different parts to induce participants to focus on collected gains (GF context) or missed chances (MF context). The control context without attentional deployment manipulation was also set. Behaviorally, compared to the control context, participants felt less regret in the GF context but more regret in the MF context. Event-related potential results showed that the GF context elicited stronger reward positivity and late positive potential (LPP) than the control context. Furthermore, openness (NEO Five-Factor Inventory) negatively predicted the amplitude of LPP in the GF context. Source localization indicated that the superior frontal gyrus showed stronger activation in the GF context than in the control context during the time window of LPP. These results suggested that focusing on collected gains was an effective way to repress regret and that the LPP component played a key role in this process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/WNR.0000000000001640DOI Listing
May 2021

The efficacy and safety of fast track surgery (FTS) in patients after hip fracture surgery: a meta-analysis.

J Orthop Surg Res 2021 Feb 27;16(1):162. Epub 2021 Feb 27.

Department of Bone and Joint Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Background: Fast track surgery (FTS) has been gradually applied in perioperative management of orthopedic surgery, but there still some research suspected that the prognosis of patients is not as expected and the cost is high, the effect of the FTS still urgently needed for support by evidence-based medicine.

Methods: We retrieved RCTs from medical research literature databases. Risk ratios (RR), standard mean difference (SMD), and 95% confidence intervals (CI) were calculated to compare the primary and safety endpoints.

Results: Overall, a total of 8886 patients were retrieved from 57 articles, of which 4448 patients (50.06%) were randomized to experimental group whereas 4438 patients (49.94%) were randomized to control group. The result showed that FTS could significantly shorten the length of stay (LOS), decrease the visual analog scale (VAS), reduce the leaving bed time and the hospitalization costs, and improve Harris hip joint function score. The incidence of complications such as respiratory system infection, urinary system infection, venous thrombus embolism (VTE), pressure sore, incision infection, constipation, and prosthesis dislocation also has been decreased significantly. Meanwhile, FTS improved patients' satisfaction apparently.

Conclusions: This meta-analysis reveals that FTS could significantly shorten the length of stay, alleviate the pain, reduce the leaving bed time and the hospitalization costs, and improve hip function. The incidence of complications also has been decreased significantly. Meanwhile, FTS has been spoken highly in patients in terms of nursing satisfaction. Its efficacy and safety were proved to be reliable.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-021-02277-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913454PMC
February 2021

Adversity Tries Friends: A Multilevel Analysis of Corporate Philanthropic Response to the Local Spread of COVID-19 in China.

J Bus Ethics 2021 Feb 17:1-28. Epub 2021 Feb 17.

UIBE Business School, University of International Business and Economics, No.10 Huixin Dongjie, Beijing, 100029 P.R. China.

We examine corporate philanthropic decisions in response to the local spread of COVID-19. From a strategic perspective, firms may proactively undertake philanthropic efforts to limit the spread of the pandemic and avoid a degraded business environment. From the perspective of non-trivial costs, increased economic uncertainty can raise concerns about business survival and lead to conservative philanthropic strategies. Following the proverb "prosperity makes friends, adversity tries them," at the provincial level, our results support the second perspective. Specifically, when the spread of the pandemic worsens in a province, local firms are less likely to make COVID-19-related donations in terms of likelihood and amount. Investors also react negatively, not only to the local spread of COVID-19 but also to COVID-19-related philanthropic donations. At the organizational level, our evidence indicates that there is at least some level of cost-benefit analysis underlying corporate philanthropic decisions. Specifically, corporate philanthropic donations, especially those made to the local business environment, are significantly affected by organizational-level factors, such as pre-existing resource availability and motives to acquire political and reputational resources. Overall, our multilevel study presents a comprehensive picture of corporate philanthropic decisions amid the COVID-19 crisis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10551-021-04745-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887540PMC
February 2021

Activation of Ovarian Taste Receptors Inhibits Progesterone Production Potentially via NO/cGMP and Apoptotic Signaling.

Endocrinology 2021 03;162(3)

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Taste receptors are not only expressed in the taste buds, but also in other nongustatory tissues, including the reproductive system. Taste receptors can be activated by various tastants, thereby exerting relatively physiologic functions. The aim of this study was to investigate the effects and potential mechanisms underlying ovarian taste receptor activation on progesterone production using saccharin sodium as the receptor agonist in a pseudopregnant rat model. Taste 1 receptor member 2 (TAS1R2) and taste 2 receptor member 31 (TAS2R31) were demonstrated to be abundantly expressed in the corpora lutea of rats, and intraperitoneal injection of saccharin sodium can activate both of them and initiate their downstream signaling cascades. The activation of these ovarian taste receptors promoted nitric oxide (NO) production via endothelial nitric oxide synthase (eNOS). NO production then increased ovarian cyclic guanosine 3',5'-monophosphate (cGMP) levels, which, in turn, decreased ovarian cyclic adenosine 3',5'-monophosphate levels. In addition, the activation of ovarian taste receptors induced apoptosis, possibly through NO and mitogen-activated protein kinase signaling. As a result, the activation of ovarian taste receptors reduced the protein expression of steroidogenesis-related factors, causing the inhibition of ovarian progesterone production. In summary, our data suggest that the activation of ovarian taste receptors inhibits progesterone production in pseudopregnant rats, potentially via NO/cGMP and apoptotic signaling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1210/endocr/bqaa240DOI Listing
March 2021

Electrically compensated, tattoo-like electrodes for epidermal electrophysiology at scale.

Sci Adv 2020 Oct 23;6(43). Epub 2020 Oct 23.

State Key Laboratory of Digital Manufacturing Equipment and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.

Epidermal electrophysiology is widely carried out for disease diagnosis, performance monitoring, human-machine interaction, etc. Compared with thick, stiff, and irritating gel electrodes, emerging tattoo-like epidermal electrodes offer much better wearability and versatility. However, state-of-the-art tattoo-like electrodes are limited in size (e.g., centimeters) to perform electrophysiology at scale due to challenges including large-area fabrication, skin lamination, and electrical interference from long interconnects. Therefore, we report large-area, soft, breathable, substrate- and encapsulation-free electrodes designed into transformable filamentary serpentines that can be rapidly fabricated by cut-and-paste method. We propose a Cartan curve-inspired transfer process to minimize strain in the electrodes when laminated on nondevelopable skin surfaces. Unwanted signals picked up by the unencapsulated interconnects can be eliminated through a previously unexplored electrical compensation strategy. These tattoo-like electrodes can comfortably cover the whole chest, forearm, or neck for applications such as multichannel electrocardiography, sign language recognition, prosthetic control or mapping of neck activities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/sciadv.abd0996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608837PMC
October 2020

Pillar[5]arene-Based Switched Supramolecular Photosensitizer for Self-Amplified and pH-Activated Photodynamic Therapy.

ACS Appl Mater Interfaces 2020 Sep 4;12(37):41038-41046. Epub 2020 Sep 4.

Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, People's Republic of China.

Photodynamic therapy (PDT) has emerged as a promising and spatiotemporally controllable cancer treatment modality. However, serious skin photosensitization during the PDT process limits the clinical application of PDT. Thus, the construction of "smart" and multifunctional photosensitizers has attracted substantial interest. Herein, we develop a mitochondria-targeting and pH-switched hybrid supramolecular photosensitizer by the host-guest interaction. The PDT efficacy of supramolecular photosensitizers can be quenched by the Förster resonance energy transfer (FRET) effect during long circulation and activated by the dissociation of supramolecular photosensitizers in an acidic tumor microenvironment, benefitting from the dynamic feature of the host-guest interaction and pH responsiveness of the water-soluble pillar[5]arene on gold nanoparticles. The rational integration of mitochondria-targeting and reductive glutathione (GSH) elimination in the hybrid switchable supramolecular photosensitizer prolongs the lifetime of reactive oxygen species generated in the PDT near mitochondria and further amplifies the PDT efficacy. Thus, the facile and versatile construction of switchable supramolecular photosensitizer offers not only the targeted and precise phototherapy but also high therapeutic efficacy, which would provide a new path for the clinic application of PDT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c10372DOI Listing
September 2020

Effects of dietary sweeteners supplementation on growth performance, serum biochemicals, and jejunal physiological functions of broiler chickens.

Poult Sci 2020 Aug 26;99(8):3948-3958. Epub 2020 Apr 26.

National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China. Electronic address:

The objective of this study was to investigate the effects of dietary 3 kinds of sweeteners supplementation on growth performance, serum biochemicals, and jejunal physiological functions of broiler chickens for 21 D. A total of one hundred ninety-two 1-day-old male Ross 308 broiler chicks were randomly divided into 4 treatments with 6 replicates for each treatment. The treatments were basal diet (CON), a basal diet supplemented with 250 mg/kg stevioside (STE), a basal diet supplemented with 100 mg/kg sucralose (SUC), and a basal diet supplemented with 600 mg/kg saccharin sodium (SAC). All birds were housed in 3-level battery cages. The results showed that dietary STE supplementation increased (P < 0.05) growth performance, serum total protein, serum albumin, and jejunal antioxidant capacity of broiler chickens. Both SUC and SAC supplementation decreased (P < 0.05) serum total protein and albumin. Dietary SAC supplementation impaired the intestinal integrity, permeability, and mucus layer of the jejunum in broiler chickens. In addition, SAC supplementation elevated (P < 0.05) the transcription expression level of jejunal bitter taste receptors and induced excessive jejunal apoptosis. Our data suggest that STE could be potentially applied as a growth-promoting and antioxidant feed additive in broiler chickens. Whereas, dietary supplementation with high level SAC has side-effects on the jejunal physiological functions of broiler chickens.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.psj.2020.03.057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597925PMC
August 2020

First report of causing leaf spot on .

Plant Dis 2020 Jul 28. Epub 2020 Jul 28.

Sichuan Agricultural University, College of Forestry, No. 211 Huimin Road, Chengdu, United States, 611130.

Chabaud is a vital ornamental and widely planted in the urban landscape of China (Lan et al. 2019). In December 2019, six of seven plants exhibited typical symptoms of leaf spot in Chengdu campus of Sichuan Agricultural University in Sichuan, China, with roughly 80% leaves per plant affected. Symptomatic leaves initially showed dark brown spots with a yellow halo at the periphery. As the disease progressed, spots gradually expanded and eventually formed necrotic spots, with the whole leaf dying when severely infected. 10 diseased leaves from different plants were collected in December 2019 and deposited in Sichuan Agricultural University Herbarium. A single spore isolation was performed following Chomnunti et al. (2014) and transferred to potato dextrose agar (PDA). Five fungal isolates were obtained from five different infected leaves respectively and deposited in Sichuan Agricultural University Culture Collection. The fungal colonies were incipiently greyish-white becoming pale brown at the margins and dark brown in the center. The reverse was the same as the obverse with radial cracking at the center. Morphological characteristics on the host were examined by light microscopy. Ascomata were semi-immersed to immersed, black, pyriform to subglobose, and measured 180-310 × 140-260 μm. The peridium comprised several layers of hyaline cells of textura angularis and measured 9-27 μm wide. Pseudoparaphyses were numerous, septate, hyaline, usually longer than asci. Asci were 8-spored, bitunicate, cylindric-clavate, and measured 50-95 × 8-11.5 μm (n=35). Ascospores were 1-septate, straight or slightly curved, hyaline, usually with globose appendages at both ends, and measured 14-18.5 × 4-6.5 μm (n=50). Conidiogenesis was not observed on PDA incubated four months at 25℃ and 95 % relative humidity on a 12-h fluorescent light/dark incubation. Morphology was consistent with the sexual stage of (Sacc.) A. Hashim. et al. described by Hashimoto et al. 2018, Tennakoon et al. 2018, and Hyde et al. 2020. The rDNA internal transcribed spacer region (ITS), 28S large subunit (LSU), 18S small subunit (SSU), translation elongation factor 1-alpha (TEF 1-α), and RNA polymerase II subunit 2 (RPB2) gene regions were amplified by using the primers ITS5/ITS4, LR0R/LR5, NS1/NS4, EF1-983F/EF1-2218R, and fRPB2-5F/fRPB2-7cR, respectively. The DNA amplification products of the representative isolate SICAUCC 20-0008 isolated from SICAU 20-0008 were sequenced and accessioned in GenBank, viz MT427731, MT427734, MT427738, MT441923, and MT441920, respectively. The sequences were compared with the GenBank database using nucleotide BLAST, and these five sequences were great identical with the sequences of the fungus (GenBank accession no. ITS, LC001732, 522/524, 99.62%; LSU, AB619009, 839/840, 99.88%; SSU, AB618690, 976/976, 100%; TEF 1-α, LC001750, 895/912, 98.14%; RPB2, MN482130, 935/935, 100%). Using phylogenetic analysis in which reference sequences collected from GenBank were included, the isolate SICAUCC 20-0008 clustered within with high bootstrap support (Fig. 1). To conduct Koch's postulates, fifteen healthy 2 to 3-year-old plants were inoculated by placing a mycelium plug from the growing margin of 15-day-old colonies upside down directly onto fresh wounds punctured with a fine needle. Five healthy plants were inoculated with a sterile agar plug as the control. Plants were incubated in a growth chamber at 25 ± 1℃ and 95% relative humidity on a 12-h fluorescent light/dark regimen. After ten days, the leaves of inoculated plants turned brown and gradually expanded and became necrotic spots, similar to symptoms observed in the field. The control plants were symptomless. The pathogen was re-isolated from leaf lesions and identified by morphological characteristics, whereas no fungus was recovered from control treatments. has been often reported as a saprobe on numerous hosts including species of , , , , , , , , and , as well as other genera (Thambugala et al. 2015, Hashimoto et al. 2018, Hyde et al. 2020). To our knowledge, this is the first report of causing leaf spot on . Some proteaceous plants and other palms are known to be infected by this fungus (Hyde et al. 2000, Taylor et al. 2000), which may result in a great threat to ornamental horticulture. Fungicides treatments should be considered to prevent the damage to plants and spread of this fungus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-05-20-1079-PDNDOI Listing
July 2020

Serum C-reactive protein to albumin ratio and mortality associated with peritoneal dialysis.

Ren Fail 2020 Nov;42(1):600-606

Department of Nephrology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Serum C-reactive protein to albumin ratio (CAR) was recently identified as a poor marker of prognosis among various populations. The current study aimed to examine the association between CAR and all-cause mortality among patients undergoing peritoneal dialysis (PD). A total of 758 patients with PD were included in this study during the period from 1 November 2005 to 28 February 2017 and followed up until 31 May 2017. The primary outcome was all-cause mortality. We used multivariate Cox proportional hazard models and Kaplan-Meier survival curves to assess the relationship between CAR and all-cause mortality in these patients. Among 758 participants, mean age was 49.1 ± 14.2 years, with 56% males and 18.6% prevalence of diabetes. Median CAR was 0.13 (interquartile range [IQR], 0.07-0.34). After 27 months (IQR, 14-40 months) of follow-up, 157 deaths had been reported. After adjusting for confounding factors, we found a significant association between serum CAR and all-cause mortality among those in the highest CAR group (hazard ratio 1.91, 95% confidence interval 1.05- 3.47,  = 0.034). In patients undergoing PD, an increase in serum CAR is independently associated with increased risk for all-cause mortality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/0886022X.2020.1783680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946068PMC
November 2020

Hirudin protects against isoproternol-induced myocardial infraction by alleviating oxidative via an Nrf2 dependent manner.

Int J Biol Macromol 2020 Nov 15;162:425-435. Epub 2020 Jun 15.

Nanjing University of Chinese Medicine, Nanjing 210023, China; Jintan Boai Hospital, Jintan 213200, Jiangsu province, China.. Electronic address:

Oxidative stress plays a critical role in the progression of myocardial injury. Increasing evidence suggests that hiruidin can treat patients with cardio-injury. However, the mechanism of hirudin against myocardial infraction remains unknown. In the present study, we evaluated the potential role and mechanism of hirudin on both isoproterenol (ISO)-induced myocardial infraction (MI) in rats and Hypoxia-Reoxygenation model in H9C2 cells. Compared with the model group, hirudin apparently decreased the levels of myocardial Creatine Kinase Isoenzyme-MB (CK-MB), lactate dehydrogenase (LDH), and alleviated myocardial histopathological changes induced by ISO injection. The underlying mechanisms were revealed by the following observations: Hirudin exerted its cardioprotective effect via restoring super oxide dismutase (SOD), attenuating reactive oxygen species (ROS) and malondialdehyde (MDA). It induced the activation of Nuclear factor erythroid 2-related factor 2 (Nrf2) signal pathway through disrupting Keap1-Nrf2 complex, thus Nrf2 translocated from cytoplasm to nucleus to regulate Nrf2-dependent gene (HO-1, SOD) expressions. Furthermore, it should be noted that hirudin restored mitochondrial membrane potential in addition to cytochrome C-related apoptosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2020.06.097DOI Listing
November 2020

Chromogranin A (CGA)-derived polypeptide (CGA) inhibits TNF-α-induced vascular endothelial hyper-permeability through SOC-related Ca signaling.

Peptides 2020 09 5;131:170297. Epub 2020 May 5.

Department of Emergency, The Third People's Hospital of Chengdu, The Second Affiliated Chengdu Clinical College of Chongqing Medical University, Chengdu, Sichuan 610031, PR China.

CGA (Vasostatin-1, VS-1) a N-terminal Chromogranin A (CGA)-derived peptide, has been shown to have a protective effect against TNF-α-induced impairment of endothelial cell integrity. However, the mechanisms of this effect have not yet been clarified. CGA (Chromofungin, CHR) is an important bioactive fragment of CGA. The present study aims to explore the protective effects of CHR on the vascular endothelial cell barrier response to TNF-α and its related Ca signaling mechanisms. EA.hy926 cells were used as a vascular endothelial culture model. The synthetic peptides CHR and CGA were assessed for their ability to suppress TNF-α-induced EA.hy926 cells hyper-permeability through Transwell® and TEER assays. Changes in [Ca] were measured through confocal laser scanning microscopy. SOC channel currents (Isoc) were measured via patch-clamp analysis. RT-PCR and western blot were used to analyze mRNA and protein expression of the transient receptor potential channels TRPC1 and TRPC4, respectively. FITC and rhodamine-phalloidin fluorescence were used to assess cell morphology and the distribution of MyPT-1 and F-actin. Compared to untreated cells, TNF-α increased the permeability of EA.hy926 cells that was inhibited by pre-treatment with CHR (10-1000 nM) in concentration-dependent manner, and the effect was most obvious at 100 nM, but CGA (100 nM) had no effect. TNF-α treatment increased the phosphorylation of MyPT-1 and stress fiber formation. CHR (10-1000 nM) pretreatment inhibited the cytoskeletal rearrangements and increased [Ca] in response to TNF-α treatment. CHR also reduced TRPC1 expression following TNF-α induction. Similar to SOC inhibitor 2-APB, CHR suppressed IP mediated SOC activation. These findings suggest that CHR inhibits TNF-α-induced Ca influx and protects the barrier function of vascular endothelial cells, and that these effects are related to the inhibition of SOC and Ca signaling by CHR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.peptides.2020.170297DOI Listing
September 2020

Letter regarding [Risk factors for intrahepatic and extrahepatic cholangiocarcinoma: A systematic review and meta-analysis].

J Hepatol 2020 06 17;72(6):1216. Epub 2020 Mar 17.

Guangxi Medical University, Nanning, Guangxi, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhep.2020.01.022DOI Listing
June 2020

Graphene oxide exposure suppresses nitrate uptake by roots of wheat seedlings.

Environ Pollut 2020 Jul 21;262:114224. Epub 2020 Feb 21.

College of Environmental Science and Engineering, Zhejiang Gongshang University, Zhejiang Province, Hangzhou, 310018, China; Instrumental Analysis Center of Zhejiang Gongshang University, Hangzhou, 310018, China. Electronic address:

Despite the large number of studies reporting the phytotoxicity of graphene-based materials, the effects of these materials on nutrient uptake in plants remain unclear. The present study showed that nitrate concentrations were significantly decreased in the roots of wheat plants treated with graphene oxide (GO) at 200-800 mg L. Non-invasive microelectrode measurement demonstrated that GO could significantly inhibit the net NO influx in the meristematic, elongation, and mature zones of wheat roots. Further analysis indicated that GO could be trapped in the root vacuoles, and that the maximal root length and the number of lateral roots were significantly reduced. Additionally, root tip whitening, creases, oxidative stress, and weakened respiration were observed. These observations indicate that GO is highly unfavorable for vigorous root growth and inhibits increase in root uptake area. At the molecular level, GO exposure caused DNA damage and inhibited the expression of most nitrate transporters (NRTs) in wheat roots, with the most significantly downregulated genes being NRT1.3, NRT1.5, NRT2.1, NRT2.3, and NRT2.4. We concluded that GO exposure decreased the root uptake area and root activity, and decreased the expression of NRTs, which may have consequently suppressed the NO uptake rate, leading to adverse nitrate accumulation in stressed plants.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2020.114224DOI Listing
July 2020

Reconstruction of a Distal Foot Skin Defect Using an Intermediate Dorsal Neurocutaneous Flap.

Orthop Surg 2020 Apr 11;12(2):442-449. Epub 2020 Feb 11.

Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan City, Hubei Province, China.

Objective: To present the use of an intermediate dorsal neurocutaneous flap for the reconstruction of defects on the distal foot.

Methods: From September 2016 to October 2018, five patients (mean age at operation 33.8 years; range, 7-70 years; female/male = 2/3) with skin defects on one of their feet caused by road-traffic accidents, electrical injury, and syndactyly correction were retrospectively reviewed. The size of the defects ranged from 2.0 cm × 1.0 cm to 5.0 cm × 3.5 cm. All patients had undergone a reconstruction surgery using intermediate dorsal neurocutaneous flap. One patient underwent a syndactyly correction, and four patients first experienced aggressive debridement. The sizes of the flaps were between 5.0 cm × 2.0 cm and 6.0 cm × 4.0 cm. The function, appearance, and pain of the injured foot were assessed using the Chinese Manchester Foot Pain and Disability Index and visual analogue scale.

Results: These five patients were systematically followed up for a mean of 15.8 months (range, 12-20 months). The donor sites were closed primarily in two cases, and skin grafts were performed in three cases. All the flaps survived with a success rate of 100%; the wounds healed well, and the color matches were excellent. Partial superficial flap necrosis occurred in one of five flaps, which was treated by dressing change using a hypertonic saline gauze. No significant problems were found at the donor site in any patient immediately afterwards or at follow-up. There were no problems in any patients associated with wearing shoes. Based on the Chinese Manchester Foot Pain and Disability Index, four patients were strongly satisfied and one was satisfied with the recovery of physical function; all the patients were strongly satisfied with the appearance of the injured foot; all five patients had an excellent score of pain intensity subscale. Except for one patient who reported mild pain, all the other patients reported no pain based on the visual analogue scale. Two typical cases are presented in this paper.

Conclusions: The intermediate dorsal neurocutaneous flap is an alternative and effective technique that can reliably cover minor- to medium-sized defects on the distal foot, toes, and web spaces. This surgical method leads to satisfactory functional recovery with minimal donor site morbidity, and no major vessels need to be sacrificed. This procedure offers an advisable option for orthopaedic surgeons to treat defects on the distal foot.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/os.12623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189044PMC
April 2020

Serum alkaline phosphatase levels correlate with long-term mortality solely in peritoneal dialysis patients with residual renal function.

Ren Fail 2019 Nov;41(1):718-725

a Department of Nephrology, The First Affiliated Hospital of Nanchang University , Nanchang , China.

Increased serum alkaline phosphatase (ALP) is predictive of a higher mortality in patients with end-stage renal disease. However, it remains unknown whether residual renal function (RRF) influences the outcome-association of serum ALP among peritoneal dialysis (PD) patients. A total of 650 incident PD patients receiving PD catheter implantation in an institute between 1 November 2005 and 28 February 2017 were retrospectively enrolled. These patients were divided into groups with and without RRF (RRF and non-RRF groups) and those with serum ALP levels in tertiles. The Kaplan-Meier method and multivariate Cox proportional hazard models were used to analyze their outcomes based on RRF and serum ALP levels. These 650 patients had a mean age of 49.4 ± 14.0 years old, their median ALP level was 74 U/L (interquartile range (IQR): 59-98). After 28-month (IQR: 14-41) follow-up, 80 patients in RRF group and 40 patients in non-RRF group died. PD patients with the highest serum ALP tertile had significant lower survival ( = .014), when compared to other patients in the RRF group. However, this relationship was not observed in patients in the non-RRF group. After multivariate adjustment, in the RRF group, patients with the highest ALP tertile had a significantly higher risk of mortality (hazard ratio (HR): 2.26, 95% confidence interval (CI): 1.06-4.82,  = .034). Each 10-U/L increase in ALP level was associated with a 4% (HR: 1.04, 95% CI: 1.00-1.08,  = .045) higher mortality risk. Higher serum ALP level is associated with increased mortality solely in PD patients with RRF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/0886022X.2019.1646662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713195PMC
November 2019

A Chest-Laminated Ultrathin and Stretchable E-Tattoo for the Measurement of Electrocardiogram, Seismocardiogram, and Cardiac Time Intervals.

Adv Sci (Weinh) 2019 Jul 21;6(14):1900290. Epub 2019 May 21.

Department of Electrical and Computer Engineering University of Texas at Austin TX 78712 USA.

Seismocardiography (SCG) is a measure of chest vibration associated with heartbeats. While skin soft electronic tattoos (e-tattoos) have been widely reported for electrocardiogram (ECG) sensing, wearable SCG sensors are still based on either rigid accelerometers or non-stretchable piezoelectric membranes. This work reports an ultrathin and stretchable SCG sensing e-tattoo based on the filamentary serpentine mesh of 28-µm-thick piezoelectric polymer, polyvinylidene fluoride (PVDF). 3D digital image correlation (DIC) is used to map chest vibration to identify the best location to mount the e-tattoo and to investigate the effects of substrate stiffness. As piezoelectric sensors easily suffer from motion artifacts, motion artifacts are effectively reduced by performing subtraction between a pair of identical SCG tattoos placed adjacent to each other. Integrating the soft SCG sensor with a pair of soft gold electrodes on a single e-tattoo platform forms a soft electro-mechano-acoustic cardiovascular (EMAC) sensing tattoo, which can perform synchronous ECG and SCG measurements and extract various cardiac time intervals including systolic time interval (STI). Using the EMAC tattoo, strong correlations between STI and the systolic/diastolic blood pressures, are found, which may provide a simple way to estimate blood pressure continuously and noninvasively using one chest-mounted e-tattoo.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.201900290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662084PMC
July 2019

A new type of DNA phosphorothioation-based antiviral system in archaea.

Nat Commun 2019 04 11;10(1):1688. Epub 2019 Apr 11.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, 430071, Wuhan, China.

Archaea and Bacteria have evolved different defence strategies that target virtually all steps of the viral life cycle. The diversified virion morphotypes and genome contents of archaeal viruses result in a highly complex array of archaea-virus interactions. However, our understanding of archaeal antiviral activities lags far behind our knowledges of those in bacteria. Here we report a new archaeal defence system that involves DndCDEA-specific DNA phosphorothioate (PT) modification and the PbeABCD-mediated halt of virus propagation via inhibition of DNA replication. In contrast to the breakage of invasive DNA by DndFGH in bacteria, DndCDEA-PbeABCD does not degrade or cleave viral DNA. The PbeABCD-mediated PT defence system is widespread and exhibits extensive interdomain and intradomain gene transfer events. Our results suggest that DndCDEA-PbeABCD is a new type of PT-based virus resistance system, expanding the known arsenal of defence systems as well as our understanding of host-virus interactions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-019-09390-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459918PMC
April 2019

Circular RNA involved in the protective effect of losartan on ischemia and reperfusion induced acute kidney injury in rat model.

Am J Transl Res 2019 15;11(2):1129-1144. Epub 2019 Feb 15.

Department of Intensive Care Unit of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences 96 Dongchuan Road, Guangzhou 510080, Guangdong Province, PR China.

Although losartan has inhibitory effects on acute kidney injury (AKI), the underlying molecular mechanisms have remained largely unclear. The expressional alteration of circular RNAs (circRNAs) was investigated in the present study to understand the therapeutic effects of losartan against AKI. AKI rat models were established by ischemia and reperfusion (I/R) treatment. Urea and creatinine levels were determined and histological features of kidney tissues examined following hematoxylin and eosin staining. Cell apoptosis was assessed by TUNEL. CircRNA profiles were obtained by RNA-Seq followed by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Expression of circRNAs was validated by quantitative RT-PCR. I/R treatment induced an increase in plasma urea and creatinine levels, abnormal kidney tubular structure, and cell apoptosis in Sprague-Dawley (SD) rats, which were effectively inhibited by pre-treatment with losartan. Further RNA-Seq analysis revealed a wide range of differentially expressed circRNAs in I/R rat kidneys, which were reversed by losartan pre-treatment. GO and KEGG analyses revealed that the circRNAs are associated with various biological processes, including the PI3K-Akt signaling pathway. Specifically, circ-Dnmt3a, circ-Akt3, circ-Plekha7, and circ-Me1 were down-regulated in AKI rats and restored by losartan. The current study provides an overview of circRNAs expression profiles based on the inhibitory effects of losartan in ischemic AKI rats.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413261PMC
February 2019

Rat mRNA expression profiles associated with inhibition of ischemic acute kidney injury by losartan.

Biosci Rep 2019 04 9;39(4). Epub 2019 Apr 9.

Department of Intensive Care Unit of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, 96 Dongchuan Road, Guangzhou 510080, Guangdong Province, P.R. China

: Losartan was reported to inhibit the progression of acute kidney injury (AKI), but little is known about the underlying pharmacological mechanisms. In the present study, the mRNA expression profiles in ischemic AKI rat kidney altered by losartan treatment were analyzed by next-generation deep sequencing technology.: Ischemia and reperfusion treatment was applied to induce AKI in Sprague-Dawley (SD) rats. The urea and creatinine contents in rat blood were measured. H&E staining was performed to evaluate the histological alteration of rat kidney tissues under a microscope. The TUNEL method was applied to analyze apoptosis in rat kidney tissues. The mRNA profiles in rat kidney were analyzed using next-generation deep sequencing. Differential gene expression was confirmed by quantitative qRT-PCR.: The rat model of AKI induced by ischemia and reperfusion showed significant increases in urea and creatinine levels, accompanied by a disrupted kidney tubular structure and renal cell apoptosis. Losartan treatment effectively inhibited the changes in urea and creatinine, tubular structure, and apoptosis in AKI rat kidney. A large number of mRNAs were found to be differentially expressed in the kidneys of AKI rats treated with losartan, which are involved in multiple processes and signaling pathways. The expression of nine differentially expressed genes such as monocyte chemoattractant protein-1 (CCL2) and suppressor of cytokine signaling 3 (SOCS3) was confirmed by qRT-PCR and Western blot.: Losartan caused significant alterations in the gene expression profile in AKI rat kidney, which mediated its anti-AKI effects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1042/BSR20181774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454018PMC
April 2019

Importance of matching soil N transformations, crop N form preference, and climate to enhance crop yield and reducing N loss.

Sci Total Environ 2019 Mar 11;657:1265-1273. Epub 2018 Dec 11.

School of Geography Science, Nanjing Normal University, Nanjing 210023, China; Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, China; Key Laboratory of Vitual Geographical Environment (VGE), Ministry of Education, Nanjing Normal University, China. Electronic address:

The preferential uptake of nitrogen (N) by plant species is generally considered to be tightly associated with soil N transformation characteristics and climatic conditions. In the present study, the yield, N recovery, and N balance of wheat (an NO-preferring crop) and rice (an NH-preferring crop) planted in two sites with significantly different soil N transformations and precipitation were compared to test the connections among soil N transformations, species-specific N preferences, crop yield, N use efficiency (NUE), N loss, and climate. Nitrogen recoveries of N applied as either an NH or NO based fertilizer in crops and soil were determined, and N losses were calculated using the N balance. The results indicated that the match (or mismatch) of crop N preference, soil N transformation characteristics, and climate conditions could significantly affect the crop yield, recovery, and loss of applied N. A crop preferring NH (e.g., rice) planted in soil with low nitrification and low pH tended to have a higher yield and NUE, and a lower N loss compared with plants growing in soil characterized by a higher nitrification rates. In contrast, NO-preferring crops (e.g. wheat) planted in soil with higher nitrification rates and lower precipitation achieved a higher yield and lower N loss than those planted in acid soil with a lower nitrification rate and higher precipitation. Replacement of ammonium N fertilizer with nitrate N fertilizer improved the NUE for NO-preferring crops (wheat) planted in arid and semi-arid regions, but not in humid regions because of the mobility of NO. Thus, a good match of crop N preference, soil N transformation characteristics, and climate conditions is critical to enhance crop yield and reduce N loss. This intimate connection needs to be taken into consideration in terms of the choice of crop species and N fertilizer type to optimize N fertilization management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2018.12.100DOI Listing
March 2019

[Application of composite tissue flaps pedicled with distal perforating branch of posterior tibial artery for repairing distal leg defects].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2019 01;33(1):75-79

Department of Trauma and Micro-orthopedics, Zhongnan Hospital of Wuhan University, Wuhan Hubei, 430071,

Objective: To investigate the clinical application and effectiveness of the composite tissue flaps pedicled with perforating branch of posterior tibial artery for repairing distal leg defects.

Methods: Between September 2014 and August 2017, 12 patients with skin and bone defects of distal leg were repaired with the composite tissue flaps pedicled with perforating branch of posterior tibial artery. There were 8 males and 4 females with an average age of 41.3 years (range, 25-66 years). The causes of injury included traffic accident injury in 7 cases, heavy crushing injury in 2 cases, tibial osteomyelitis with soft tissue ulcer and necrosis in 2 cases, and bone and soft tissue defect after resection of bone tumor in 1 case. Eight patients underwent primary repair, and 4 patients underwent second-stage repair. The size ranged from 6 cm×4 cm to 10 cm×7 cm in skin flap, from 4.0 cm×2.5 cm to 8.0 cm×6.0 cm in muscle flap, and from 4 cm×2 cm×2 cm to 5 cm×4 cm×4 cm in tibial bone flap. Tibial defects of the donor region were repaired by autologous iliac bone grafting, and the wounds were sutured directly in 7 cases and repaired by autologous skin grafting in 5 cases.

Results: All composite tissue flaps survived and both the recipient and the donor wounds healed primarily. All patients were followed up 6-12 months, with an average of 10.8 months. The appearance, color, texture of the composite tissue flaps and ankle function were satisfactory. X-ray films showed that the bone flap at the tibia defect and the ilium graft at the donor site both healed well at 6 months after operation.

Conclusion: The composite tissue flaps pedicled with perforating branch of posterior tibial artery has abundant blood, and it is a good donor region for repairing the distal leg defects combined with circumscribed bone defect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7507/1002-1892.201805093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8337253PMC
January 2019
-->