Publications by authors named "Siyi Huang"

31 Publications

New Precordial T wave Inversions in Hospitalized Patients.

Am J Med 2021 Nov 20. Epub 2021 Nov 20.

Division of Cardiovascular Medicine, University of California San Francisco, Fresno, California.

Background: The incidence of precordial T changes has been described in athletes and in specific populations, while the etiology in a large patient population admitted to the hospital has not previously been reported.

Methods: All ECGs read by the same physician with new (compared to prior ECGs) or presumed new (no prior ECGs) precordial T wave inversions of >1 mm (0.1 mV) in multiple precordial leads were retrospectively reviewed and various ECG, patient-related and imaging parameters assessed. 226 patients and their ECGs were initially selected for analysis. Of these, 35 were eliminated leaving 191 for the final analysis.

Results: Patients and their ECGs were divided into 5 groups based on diagnosis and incidence including Wellens' syndrome, takotsubo, type 2 myocardial infarction, other (including multiple diagnoses) and unknown. While subtle differences including number of T inversion leads, depth of T waves, QTc intervals and other variables were present between some groups, diagnosis in individual cases required appropriate clinical, laboratory and/or imaging studies. For example, although Wellens' syndrome was identified in <20% of cases, a presenting history of chest discomfort with precordial T changes either on the admission or next day ECG was highly sensitive and specific for this diagnosis. In some cases, Type 2 myocardial infarction can also have a Wellens' like ECG phenotype without significant left anterior descending disease.

Conclusions: Precordial T wave changes in hospitalized patients have various etiologies and, in individual cases, the changes on the ECG alone cannot easily distinguish the presumptive diagnosis and additional data are required.
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http://dx.doi.org/10.1016/j.amjmed.2021.10.030DOI Listing
November 2021

Is positron emission tomography enough to rule out cardiac sarcoidosis? A case report.

Eur Heart J Case Rep 2021 Sep 13;5(9):ytab300. Epub 2021 Sep 13.

Cardiology, University of California, San Francisco, Fresno, 2335 E Kashian Ln, Fresno, CA 93701, USA.

Background: Cardiac sarcoidosis (CS) is associated with poor prognosis, yet the clinical diagnosis is often challenging. Advanced cardiac imaging including cardiac magnetic resonance (CMR) and positron emission tomographic (PET) have emerged as useful modalities to diagnose CS.

Case Summary: A 66-year-old woman presented with palpitations. A 24-h Holter monitor detected a high premature ventricular contraction burden of 25.6%. She underwent two transthoracic echocardiograms; both showed normal results. Stress perfusion CMR did not show any evidence of ischaemic aetiology; however, myocardial lesions detected by late gadolinium enhancement (LGE) imaging raised suspicion for CS. While there was no myocardial uptake of fluorodeoxyglucose (FDG) in subsequent cardiac PET, high FDG uptake was seen in hilar lymph nodes. Lymph node biopsy confirmed the diagnosis of sarcoidosis.

Discussion: Cardiac magnetic resonance and PET imaging are designed to evaluate different aspects CS pathophysiology. The characteristic LGE in the absence of increased FDG uptake suggested inactive CS with residual myocardial scarring.
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http://dx.doi.org/10.1093/ehjcr/ytab300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453392PMC
September 2021

Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes.

Nat Commun 2021 08 17;12(1):4995. Epub 2021 Aug 17.

Institute for Medical Engineering & Science, MIT, Cambridge, MA, USA.

A cell's phenotype and function are influenced by dynamic interactions with its microenvironment. To examine cellular spatiotemporal activity, we developed SPACECAT-Spatially PhotoActivatable Color Encoded Cell Address Tags-to annotate, track, and isolate cells while preserving viability. In SPACECAT, samples are stained with photocaged fluorescent molecules, and cells are labeled by uncaging those molecules with user-patterned near-UV light. SPACECAT offers single-cell precision and temporal stability across diverse cell and tissue types. Illustratively, we target crypt-like regions in patient-derived intestinal organoids to enrich for stem-like and actively mitotic cells, matching literature expectations. Moreover, we apply SPACECAT to ex vivo tissue sections from four healthy organs and an autochthonous lung tumor model. Lastly, we provide a computational framework to identify spatially-biased transcriptome patterns and enriched phenotypes. This minimally perturbative and broadly applicable method links cellular spatiotemporal and/or behavioral phenotypes with diverse downstream assays, enabling insights into the connections between tissue microenvironments and (dys)function.
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http://dx.doi.org/10.1038/s41467-021-25279-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371137PMC
August 2021

Loss of WTAP Impairs Early Parthenogenetic Embryo Development.

Animals (Basel) 2021 Jun 4;11(6). Epub 2021 Jun 4.

Jilin Provincial Key Laboratory of Animal Model, College of Animal Science, Jilin University, Changchun 130062, China.

mA is one of the most common and abundant modifications of RNA molecules present in eukaryotes. The methyltransferase complex, consisting of methyltransferase-like 3 (METTL3), METTL14, and WTAP, is responsible for the mA modification of RNA. WTAP was identified as an mRNA splicing regulator. Its role as a regulatory subunit of the mA methyltransferase complex in embryonic development remains largely unknown. To investigate the role of WTAP in porcine early embryonic development, si-WTAP was microinjected into porcine parthenogenetic zygotes. WTAP knockdown significantly reduced the blastocyst rate and global mA levels, but did not affect the cleavage rate. Betaine was supplemented into the in vitro culture (IVC) to increase the mA levels. Betaine significantly increased the global mA levels but did not affect the blastocyst rate. Furthermore, the pluripotency genes, including , , and , were downregulated following WTAP knockdown. The apoptotic genes and were upregulated, while the anti-apoptotic gene was downregulated in WTAP knockdown blastocysts. TUNEL staining revealed that the number of apoptotic cells was significantly increased following WTAP knockdown. Our study indicated that WTAP has an indispensable role in porcine early embryonic development.
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http://dx.doi.org/10.3390/ani11061675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229322PMC
June 2021

Compact Biomimetic Hair Sensors Based on Single Silicon Nanowires for Ultrafast and Highly-Sensitive Airflow Detection.

Nano Lett 2021 06 29;21(11):4684-4691. Epub 2021 May 29.

Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou 215123, Jiangsu, People's Republic of China.

Wearable sensors that can mimic functionalities of human bodies have attracted intense recent attention. However, research on wearable airflow sensors is still lagging behind. Herein, we report a biomimetic hair sensor based on a single ultralong silicon nanowire (SiNW-BHS) for airflow detection. In our device, the SiNW can provide both mechanical and electrical responses in airflow, which enables a simple and compact design. The SiNW-BHSs can detect airflow with a low detection limit (<0.15 m/s) and a record-high response speed (response time <40 ms). The compact design of the SiNW-BHSs also enables easy integration of an array of devices onto a flexible substrate to mimic human skin to provide comprehensive airflow information including wind speed, incident position, incident angle, and so forth. This work provides novel-designed BHSs for ultrafast and highly sensitive airflow detection, showing great potential for applications such as e-skins, wearable electronics, and robotics.
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http://dx.doi.org/10.1021/acs.nanolett.1c00852DOI Listing
June 2021

Effect of Graded-Indium-Content Superlattice on the Optical and Structural Properties of Yellow-Emitting InGaN/GaN Quantum Wells.

Materials (Basel) 2021 Apr 9;14(8). Epub 2021 Apr 9.

Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.

We have improved the material quality of the high indium composition InGaN/GaN multiple quantum wells (MQWs) grown on free-standing GaN substrates using the graded-indium-content superlattice. We found that by adopting a graded-indium-content superlattice structure, the spectral FWHM of the yellow emitting InGaN/GaN MQW was reduced from 181 meV to 160 meV, and the non-radiative recombination lifetime increased from 13 ns to 44 ns. Besides, the graded-indium-content superlattice can mitigate strain relaxation in high indium composition MQWs as shown by the TEM diffraction patterns.
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http://dx.doi.org/10.3390/ma14081877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069205PMC
April 2021

Hydroxyurea affects in vitro porcine oocyte maturation through increased apoptosis and oxidative stress.

Biosci Rep 2021 04;41(4)

Jilin Provincial Key Laboratory of Animal Model, College of Animal Science, Jilin University, Changchun 130062, China.

Hydroxyurea (HU) is an FDA-approved drug used to treat a variety of diseases, especially malignancies, but is harmful to fertility. We used porcine oocytes as an experimental model to study the effect of HU during oocyte maturation. Exposure of cumulus-oocyte complexes (COCs) to 20 µM (P<0.01) and 50 µM (P<0.001) HU reduced oocyte maturation. Exposure to 20 µM HU induced approximately 1.5- and 2-fold increases in Caspase-3 (P<0.001) and P53 (P<0.01) gene expression levels in cumulus cells, respectively, increased Caspase-3 (P<0.01) and P53 (P<0.001) protein expression levels in metaphase II (MII) oocytes and increased the percentage of apoptotic cumulus cells (P<0.001). In addition, HU decreased the mitochondrial membrane potential (Δφm) (P<0.01 and P<0.001) and glutathione (GSH) levels (P<0.01 and P<0.001) of both cumulus cells and MII oocytes, while increasing their reactive oxygen species (ROS) levels (P<0.001). Following parthenogenetic activation of embryos derived from MII oocytes, exposure to 20 µM HU significantly reduced total blastocyst cell numbers (P<0.001) and increased apoptosis of blastocyst cells (P<0.001). Moreover, HU exposure reduced the rate of development of two-celled, four- to eight-celled, blastocyst, and hatching stages after parthenogenetic activation (P<0.05). Our findings indicate that exposure to 20 µM HU caused significant oxidative stress and apoptosis of MII oocytes during maturation, which affected their developmental ability. These results provide valuable information for safety assessments of HU.
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http://dx.doi.org/10.1042/BSR20203091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062957PMC
April 2021

Preparation of CeZrO/SnInS composite photocatalyst and its degradation of typical antibiotic pollutants.

Environ Sci Pollut Res Int 2021 Jun 1;28(22):28024-28037. Epub 2021 Feb 1.

Research Institute of Photocatalysis, College of Environment and Resources, Fuzhou University, Fuzhou, 350116, China.

Considering the high environmental risk, the remediation of antibiotic pollutants attracted numerous attentions. In this work, a novel photocatalyst, CeZrO/SnInS, was fabricated by in situ precipitation and hydrothermal method and then applied to the degradation of norfloxacin under the irritation of visible light. The SEM, TEM, XRD, XPS, and electrochemical results clearly showed that the n-type heterojunction between CeZrO and SnInS was successfully constructed, which greatly reduces the recombination of the photogenic electron and holes, leading to the improvement of photocatalytic performance and stability (recycled over eight times). Besides, the CeZrO/SnInS composite also exhibited good ability to mineralize norfloxacin. Under the optimal condition (pH 3, 1 g L of 10% CeZrO/SnInS, and 8 mg L of initial norfloxacin concentration), norfloxacin could be fully and rapidly degraded in 60 min, and completely mineralized in 4 h (99.3 ± 1.7%). LC-QTOF-MS results evidently displayed eight intermediates during norfloxacin degradation. In addition, with the attack of the reactive oxygen species (h, •OH, and •O), norfloxacin could be effectively decomposed via deoxygenation, hydroxylation, and carboxylation reactions. Notably, compared to photodegradation, the photocatalytic process could completely eliminate the norfloxacin from water because it could avoid the accumulation of toxic byproducts.
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http://dx.doi.org/10.1007/s11356-021-12610-9DOI Listing
June 2021

Rapidly photocatalytic mineralization of typical veterinary drugs with the SnO/SnInS composite.

Chemosphere 2021 May 28;271:129452. Epub 2020 Dec 28.

Research Institute of Photocatalysis, College of Environment & Resources, Fuzhou University, Fuzhou, 350116, China. Electronic address:

Considering the high environmental risk, the remediation of veterinary drug pollutants aroused numerous concerning. In this paper, a novel photocatlyst, SnO/SnInS, was fabricated by in situ precipitation and hydrothermal method and then employed to simulate photocatalytic degradation of olaquindox under visible light. The SEM, TEM, XRD, XPS and electrochemical results clearly showed that the n-type heterojunction between SnO and SnInS was successfully constructed, which greatly reduce the recombination of the photogenic electron and holes, leading to the improvement of photocalytic performance and stability (recycled over 10 times). Besides, the SnO/SnInS composite also exhibited good ability to mineralize the olaquindox. Under the optimal condition (pH of 3, 1 g L of 30 wt% SnO/SnInS and 10 mg L of initial olaquindox concentration), the olaquindox could be fully and rapidly degraded in 25 min, and completely mineralized in 2 h (99.3 ± 1.7%). LC-QTOF-MS analysis evidently displayed 10 intermediates during the olaquindox degradation. In addition, with the attack of the reactive oxygen species (h, •OH and •O), olaquindox could be effectively decomposed via deoxygenation, hydroxylation and carboxylation reactions. Importantly, compared to photodegradation, the photocatalytic process was an ideal way to eliminate the olaquindox form water because it could avoid the accumulation of toxic byproducts.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129452DOI Listing
May 2021

Lymph nodes are innervated by a unique population of sensory neurons with immunomodulatory potential.

Cell 2021 01 16;184(2):441-459.e25. Epub 2020 Dec 16.

The Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA; Department of Immunology & HMS Center for Immune Imaging, Harvard Medical School, Boston, MA 02115, USA. Electronic address:

Barrier tissue immune responses are regulated in part by nociceptors. Nociceptor ablation alters local immune responses at peripheral sites and within draining lymph nodes (LNs). The mechanisms and significance of nociceptor-dependent modulation of LN function are unknown. Using high-resolution imaging, viral tracing, single-cell transcriptomics, and optogenetics, we identified and functionally tested a sensory neuro-immune circuit that is responsive to lymph-borne inflammatory signals. Transcriptomics profiling revealed that multiple sensory neuron subsets, predominantly peptidergic nociceptors, innervate LNs, distinct from those innervating surrounding skin. To uncover LN-resident cells that may interact with LN-innervating sensory neurons, we generated a LN single-cell transcriptomics atlas and nominated nociceptor target populations and interaction modalities. Optogenetic stimulation of LN-innervating sensory fibers triggered rapid transcriptional changes in the predicted interacting cell types, particularly endothelium, stromal cells, and innate leukocytes. Thus, a unique population of sensory neurons monitors peripheral LNs and may locally regulate gene expression.
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http://dx.doi.org/10.1016/j.cell.2020.11.028DOI Listing
January 2021

Procyanidin B1 promotes in vitro maturation of pig oocytes by reducing oxidative stress.

Mol Reprod Dev 2021 01 25;88(1):55-66. Epub 2020 Nov 25.

Department of Laboratory Animal Science, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun, China.

Oxidative stress negatively affects the in vitro maturation (IVM) of oocytes. Procyanidin B1 (PB1) is a natural polyphenolic compound that has antioxidant properties. In this study, we investigated the effect of PB1 supplementation during IVM of porcine oocytes. Treatment with 100 μM PB1 significantly increased the MII oocytes rate (p <0.05), the parthenogenetic (PA) blastocyst rate (p <0.01) and the total cell number in the PA blastocyst (p < 0.01) which were cultured in regular in vitro culture (IVC) medium. The PA blastocyst rate of regular MII oocytes activated and cultured in IVC medium supplemented with 100 and 150 μM PB1 significantly increased compared with control (p < 0.01 and p < 0.05). We also evaluated the reactive oxygen species (ROS) levels, mitochondrial membrane potential (Δψm) levels, glutathione (GSH) levels, and apoptotic levels in MII oocytes and cumulus cells following 100 μM PB1 treatment. The results showed that the PB1 supplementation decreased ROS production and apoptotic levels. In addition, PB1 was found to increase Δψm levels and GSH levels. In conclusion, PB1 inhibited apoptosis of oocytes and cumulus cells by reducing oxidative stress. Moreover, PB1 improved the quality of oocytes and promoted PA embryo development. Taken together, our results suggest that PB1 is a promising antioxidant additive for IVM of oocytes.
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http://dx.doi.org/10.1002/mrd.23440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894521PMC
January 2021

LncWNT3-IT affects the proliferation of Sertoli cells by regulating the expression of the WNT3 gene in goat testis.

Reprod Domest Anim 2020 Sep 11;55(9):1061-1071. Epub 2020 Aug 11.

College of Animal Science and Technology, Southwest University, Chongqing, China.

The proliferation and differentiation ability of testicular Sertoli cells directly affects spermatogenesis and male reproductive development. WNT proteins are involved in the regulation of cell proliferation, differentiation and spermatogenesis. Therefore, to study whether lncRNAs, which regulate the expression of WNT proteins during cell proliferation and differentiation, are worthwhile. In this study, testicular tissue from the Dazu black goat (Capra, goat, Chongqing, China) at neonatal time (less than 7 days old), early puberty time (45 days old) and sexual maturity time (90 days old) at three ages was subjected to high-throughput sequencing to predict testicular growth and development associated with WNT lncRNA. The final screening of lncWNT3-IT may be targeted to regulate the expression of WNT3. At the same time, the expression of WNT3 was verified by lncWNT3-IT by paraffin sectioning, fluorescence in situ hybridization, interference, overexpression, cytotoxicity assay, Western blotting and qPCR. The following results were obtained: lncWNT3-IT was expressed in the testicular Sertoli cells and played a role in the Sertoli cell cytoplasm. Fluorescence in situ hybridization localization analysis showed that lncWNT3-IT positively regulated the expression of WNT3, and through cell viability and cell proliferation experiments, it was found that the expression of lncWNT3-IT assisted in Sertoli cell proliferation. In summary, lncWNT3-IT can influence the proliferation of Sertoli cells by positively regulating the expression of WNT3.
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http://dx.doi.org/10.1111/rda.13738DOI Listing
September 2020

Galangin promotes cell apoptosis through suppression of H19 expression in hepatocellular carcinoma cells.

Cancer Med 2020 08 2;9(15):5546-5557. Epub 2020 Jun 2.

Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, China.

Background: Galangin has been extensively studied as the antitumor agent in various cancers. However, the effect of galangin in hepatocellular carcinoma (HCC) remains elusive.

Methods: Using RNA sequencing, the differential expression of lncRNA in human HCC cell line with highly metastatic potential (MHCC97H) cells treated with galangin was investigated. Furthermore, H19 expression pattern was also determined in MHCC97H cells following treatment with galangin. In addition, knockdown and overexpression of H19 was performed to analyze the effect of the expression pattern of H19 on cell apoptosis, cell cycle, migration, and invasion in HCC cells. Moreover, the in vivo effect of galangin on tumor development was also determined in nude mice. In order to analyze loss expression of H19 in vivo, clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) was used.

Results: Total of 50 lncRNAs were significantly differentially expressed in MHCC97H cells treated with galangin. Besides, the expression of H19 was markedly reduced following treatment with galangin in MHCC97H cells. Compared to the Control group, the galangin-treated group inhibited cell migration and invasion. Knockdown of H19 expression showed increased cell apoptosis and decreased invasion. In addition, RNA-seq data also identified 161 mRNA which was significantly differentially expressed following treatment with galangin. To further determine the underlying mechanism, p53 protein was analyzed. Notably, the results indicated that knockdown of H19 and miR675 induced the expression of p53, eventually promoting cell apoptosis in MHCC97H cells. These results indicated that galangin promoted cell apoptosis through reduced the expression of H19 and miR675 in MHCC97H cells. The in vivo result showed that compared to the Con, tumor growth was remarkably suppressed with loss expression of H19.

Conclusion: Our data suggested that galangin has a crucial role in hepatocarcinogenesis through regulating the expression pattern of H19.
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http://dx.doi.org/10.1002/cam4.3195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7402821PMC
August 2020

Suppression of substrate mode in GaN-based green laser diodes.

Opt Express 2020 May;28(10):15497-15504

Parasitic substrate mode readily appears in GaN-based laser diodes (LDs) because of insufficient optical confinement, especially for green LDs. Substrate modes affect the behavior of a LD severely, including the laser beam quality, the optical output power, the longitudinal mode stability, and the maximum modulation speed. In this article, systematic studies on the n-cladding layer (CL) design to suppress the substrate mode of GaN-based green LDs were carried out. We established a contour map to describe the relationship between the optical confinement (determined by the thickness and the refractive index) of n-CL and the substrate mode intensity by simulating the near-field pattern and the far-field pattern. We found that it was difficult to obtain the Gaussian-shape far-field pattern using AlGaN as a cladding layer due to the appearance of cracks induced by tensile strain. However, this can be realized by introducing quaternary AlInGaN as a cladding layer since refractive index and strain can be tuned separately for quaternary alloy.
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http://dx.doi.org/10.1364/OE.389880DOI Listing
May 2020

Biomechanics study of a 3D printed sacroiliac joint fixed modular hemipelvic endoprosthesis.

Clin Biomech (Bristol, Avon) 2020 04 28;74:87-95. Epub 2020 Feb 28.

Musculoskeletal Tumor Center, People's Hospital, Peking University, Xizhimen Nan 11#, Xicheng District, Beijing 100044, China. Electronic address:

Background: Reconstructing pelvic type II + III defect caused by bone tumors is challenging. The purpose of this study was to explore the in vitro biomechanical properties of a reconstructed pelvis after periacetabular resection using three-dimensional (3D) printed sacroiliac joint (SIJ) fixed modular hemipelvic endoprosthesis.

Methods: Type II/II + III pelvic resection was simulated on an artificial pelvic model. The bilateral acetabulum and pubis were constrained, and the pelvis was maintained in a human physiological standing position. A vertically continuous linear load was applied on the upper face of S1 until obvious unloading or fixed failure occurred. A noncontact optical 3D strain measuring system was used to measure the strains and displacements at the selected area.

Findings: The strain at the points of interest did not obviously differ between the intact and reconstructed pelvis models. The difference in the displacement on the reconstructed side was 0.237 mm, and that on the contralateral side was 0.245 mm. The maximum differences in the displacement at the acetabulum were 0.209 mm (vertical) and 0.324 mm (horizontal). A crack at the superior rim of the contralateral acetabulum occurred, and failure loading of 7.126 kN.

Interpretation: The prosthesis in this study showed satisfactory mechanical properties and structural stability. According to the mechanical evaluations, the 3D printed sacroiliac-stabilized hemipelvic endoprosthesis can be used to reconstruct a stable acetabular structure, and there was little influence on the mechanical properties of the surrounding bone structures. The prosthesis design is reasonable, and the mechanical distribution on the reconstructed side was similar to that on the contralateral side.
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http://dx.doi.org/10.1016/j.clinbiomech.2020.02.014DOI Listing
April 2020

Ultraminiaturized Stretchable Strain Sensors Based on Single Silicon Nanowires for Imperceptible Electronic Skins.

Nano Lett 2020 04 11;20(4):2478-2485. Epub 2020 Mar 11.

Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory of Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu 215123, People's Republic of China.

Miniaturized stretchable strain sensors are key components in E-skins for applications such as personalized health-monitoring, body motion perception, and human-machine interfaces. However, it remains a big challenge to fabricate miniaturized stretchable strain sensors with high imperceptibility. Here, we reported for the first time novel ultraminiaturized stretchable strain sensors based on single centimeter-long silicon nanowires (cm-SiNWs). With the diameter of the active materials even smaller than that of spider silks, these sensors are highly imperceptible. They exhibit a large strain sensing range (>45%) and a high durability (>10 000 cycles). Their optimum strain sensing ranges could be modulated by controlling the prestrains of the stretchable cm-SiNWs. On the basis of this capability, sensors with appropriate sensing ranges were chosen to respectively monitor large and subtle human motions including joint motion, swallow, and touch. The strategy of applying single cm-SiNWs in stretchable sensors would open new doors to fabricate ultraminiaturized stretchable devices.
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http://dx.doi.org/10.1021/acs.nanolett.9b05217DOI Listing
April 2020

Remediation of organic arsenic contaminants with heterogeneous Fenton process mediated by SiO-coated nano zero-valent iron.

Environ Sci Pollut Res Int 2020 Apr 25;27(11):12017-12029. Epub 2020 Jan 25.

Fujian Provincial Engineering Research Center for High-value Utilization Technology of Plant Resources, College of Environment & Resources, Fuzhou University, No. 2 Xueyuan Road, Shangjie Town, Minhou County, Fuzhou, 350116, Fujian, China.

Given their considerable solubility in water and potentially high toxicity to human health, organoarsenic compounds have become an emerging contaminant. Herein, a heterogeneous Fenton process mediated by SiO-coated nano zero-valent iron (SiO-nZVI) was employed to simultaneously remove the p-arsanilic acid (p-ASA, a typical organoarsenic compound) and the released arsenic. The initial pH value significantly influenced on the degradation of p-ASA and at the optimal pH (3.0), p-ASA (10 mg L) could be completely oxidized to As(V), NH, and plentiful phenolic compounds such as phenol and p-hydroquinone via the cleavage of C-N and C-As bonds within 60 min in pure water. Meanwhile, although the formed lepidocrocite and magnetite on the surface of SiO-nZVI significantly limited the reutilization, they played a vital role in the adsorption of the released As(V) and the residual arsenic levels in the effluent were as low as 0.031 mg L, meeting both the drinking water standard of the World Health Organization (WHO) and the surface water standard of China (0.05 mg L). Furthermore, high-level dissolved organic matters (DOM) (> 2 mg C L) exhibited strong interference with both the oxidation of p-ASA and adsorption of arsenic, but the interference could be eliminated by increasing the SiO-nZVI dosage or adding HO. Importantly, this system could completely remediate p-ASA in a short time and simultaneously avoid the secondary pollution caused by inorganic arsenic, which was significant for the remediation of organoarsenic pollutants in swine wastewater.
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http://dx.doi.org/10.1007/s11356-020-07808-2DOI Listing
April 2020

Multicentric Castleman disease with TAFRO syndrome and Sjögren's.

Clin Case Rep 2019 Dec 24;7(12):2388-2392. Epub 2019 Oct 24.

Department of Internal Medicine University of California, San Francisco-Fresno Fresno CA USA.

We describe a patient with Castleman's disease with TAFRO syndrome and concurrent Sjögren's syndrome and investigate whether the autoimmune process may have accelerated the onset of her Castleman's disease. Patient was treated with R-CVP therapy with remission of symptoms although there is no current standard treatment.
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http://dx.doi.org/10.1002/ccr3.2502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935625PMC
December 2019

LncNONO-AS regulates AR expression by mediating NONO.

Theriogenology 2020 Mar 7;145:198-206. Epub 2019 Nov 7.

College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.

Spermatogenesis and healthy testicular development are prerequisites for male reproductive function. Androgen receptor (AR), an important receptor in testicular sertoli cells, is involved in androgen specific response and its dysfunction will lead to abnormal sperm development, resulting in male infertility. NONO (non-POU-domain-containing octamer binding protein) can act as a coactivator to enhance the transcription of AR, while AR may be regulated by NONO in testicular sertoli cells. LncRNAs are involved in almost every step of spermatogenesis. However, there are few studies focus on the relationship between lncRNAs and spermatogenesis in goat testis. Therefore, in this research, high throughput sequencing and bioinformatics analysis were performed on testicular tissues of Dazu black goats at different stages of development to obtain the target NONO lncRNA. It's called lncNONO-AS. This study further explored the biological functions of lncRNA through RNA pull down, overexpression, interference, fluorescence quantification, Western blot and other techniques on the basis of in vitro culture of testis sertoli cells, and we got the following results: The gene expression levels of NONO and AR in lncNONO-AS overexpression group were significantly higher than that in the empty vector group (P < 0.01). Compared with the untreated negative control group, the expression of NONO decreased from 1.00 to 0.68 (P < 0.01), and the expression of AR decreased from 1.01 to 0.34 (P < 0.01). The results showed that lncNONO-AS could regulate the expression of AR by mediating the expression of NONO.
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http://dx.doi.org/10.1016/j.theriogenology.2019.10.025DOI Listing
March 2020

Biomechanical comparison of a 3D-printed sacrum prosthesis versus rod-screw systems for reconstruction after total sacrectomy: A finite element analysis.

Clin Biomech (Bristol, Avon) 2019 Dec 20;70:203-208. Epub 2019 Oct 20.

Musculoskeletal Tumor Center, Peking University People's Hospital, Beijing, China. Electronic address:

Background: Reconstruction after total sacrectomy is a difficult problem in the field of orthopedic oncology. Current reconstruction methods have not completely solved the problems associated with instrumentation failure. The purpose of this study was to evaluate the biomechanical properties of a 3D-printed total sacrum prosthesis and to conduct biomechanical comparisons between the total sacrum prosthesis and rod-screw systems for lumbosacral reconstruction after total sacrectomy.

Methods: Three types of reconstruction were explored, and corresponding finite element models were simulated: four-rod reconstruction, four-rod plus anterior column reconstruction, and 3D-printed total sacrum prosthesis reconstruction. A vertical load of 600 N was applied to the L4 vertebra, and the bilateral acetabula were set as the boundary with six degrees of freedom fixed, simulating the bipedal standing position.

Findings: The order of the reconstructions according to decreasing maximum von Mises stress was as follows: four-rod reconstruction > four-rod plus anterior column reconstruction >3D-printed total sacrum prosthesis reconstruction. The order of reconstructions according to decreasing L5 shift-down displacement was as follows: four-rod reconstruction >3D-printed total sacrum prosthesis reconstruction > four-rod plus anterior column reconstruction.

Interpretation: Compared with the rod-screw systems, the total sacrum prosthesis reconstruction has the biomechanical advantages of a more uniform stress distribution, a lower peak stress and better stability and can thus serve as an alternative choice for reconstruction after total sacrectomy.
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http://dx.doi.org/10.1016/j.clinbiomech.2019.10.019DOI Listing
December 2019

Correlation between MCP-1-2518A/G polymorphism and the risk of Alzheimer's disease.

J Neural Transm (Vienna) 2018 12 3;125(12):1781-1786. Epub 2018 Oct 3.

Department of Biopharmaceutical, Yulin Normal University, Yulin, 537000, China.

The -2518A/G polymorphism in monocyte chemotactic protein-1 (MCP-1) has been extensively investigated for association with Alzheimer's disease (AD); however, the results from different studies are inconsistent. The aim of this study was to draw an accurate conclusion of the association. All eligible case-control studies were searched in PubMed, Embase, Chinese National Knowledge Infrastructure, China Biological Medicine Databases, and Wanfang Databases. Eight case-control studies with a total of 2370 cases and 2413 controls were eligible to be included in this meta-analysis. The association was evaluated by calculating the odds ratios (ORs) with the corresponding 95% confidence intervals (CIs). Overall, there was no significant association between MCP-1-2518A/G polymorphism and AD risk in all genetic models (the allele model G vs. A: OR = 1.15, 95% CI 0.92-1.45, p = 0.22; the co-dominant model GG vs. AA: OR = 1.38, 95% CI 0.80-2.36, p = 0.25; the dominant model AG + GG vs. AA: OR = 1.14, 95% CI 0.89-1.46, p = 0.31; the recessive model GG vs. AG + AA: OR = 1.35, 95% CI 0.87-2.09, p = 0.18). In subgroup analysis by ethnicity, a significant difference was not detected in both Caucasians and Asians. In allele model (G vs. A), the required sample size of 31858 was calculated by applying trial sequential analysis. Cumulative z curve is always below the trial sequential monitoring boundary and is nominally statistically significant (Z = 1.96). A consistent result was obtained in other genetic models. In summary, the present meta-analysis suggests that MCP-1-2518A/G polymorphism may not be associated with genetic susceptibility of AD in general population, but the association remains indeterminate due to the insufficient evidence.
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http://dx.doi.org/10.1007/s00702-018-1936-7DOI Listing
December 2018

Association of CD14-260 (-159) C/T and Alzheimer's disease: systematic review and trial sequential analyses.

J Neural Transm (Vienna) 2018 09 20;125(9):1313-1318. Epub 2018 Jun 20.

Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China.

Current studies have evaluated the association between CD14-260 (also known as -159) C/T polymorphism and Alzheimer's disease (AD) susceptibility. However, the association remains inconclusive. The aim of this study was to draw an accurate conclusion of the association. The literature search was conducted using PubMed, Embase, Chinese National Knowledge Infrastructure, China Biological Medicine Database, and Wanfang Databases for related articles. Four case-control studies with a total of 868 cases and 766 controls were eligible to be included in this meta-analysis. The association was evaluated by calculating the odds ratios (ORs) with the corresponding 95% confidence intervals (CIs). Overall, there was no significant association between CD14-260C/T polymorphism and AD risk in all genetic models (the allele model T vs. C: OR = 1.06, 95% CI 0.92-1.21, p = 0.44; the homozygous model TT vs. CC: OR = 1.09, 95% CI 0.83-1.44, p = 0.53; the heterozygote model CT vs. CC: OR = 0.95, 95% CI 0.75-1.22, p = 0.71; the dominant model TT + CT vs. CC: OR = 1.05, 95% CI 0.84-1.32, p = 0.66; the recessive model TT vs. CT + CC: OR = 1.14, 95% CI 0.92-1.43, p = 0.24). The sample size of 5064 was calculated by applying trial sequential analysis. Cumulative z curve does not cross trial sequential monitoring boundary. In conclusion, the present meta-analysis suggests that the CD14-260C/T polymorphism may not be associated with genetic susceptibility of AD, but the association remains indeterminate due to the insufficient evidence.
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http://dx.doi.org/10.1007/s00702-018-1896-yDOI Listing
September 2018

[Development and current situation of reconstruction methods following total sacrectomy].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2018 05;32(5):513-518

Musculoskeletal Tumor Center, Peking University People's Hospital, Beijing, 100044,

Objective: To review the development of the reconstruction methods following total sacrectomy, and to provide reference for finding a better reconstruction method following total sacrectomy.

Methods: The case reports and biomechanical and finite element studies of reconstruction following total sacrectomy at home and abroad were searched. Development and current situation were summarized.

Results: After developing for nearly 30 years, great progress has been made in the reconstruction concept and fixation techniques. The fixation methods can be summarized as the following three strategies: spinopelvic fixation (SPF), posterior pelvic ring fixation (PPRF), and anterior spinal column fixation (ASCF). SPF has undergone technical progress from intrapelvic rod and hook constructs to pedicle and iliac screw-rod systems. PPRF and ASCF could improve the stability of the reconstruction system.

Conclusion: Reconstruction following total sacrectomy remains a challenge. Reconstruction combining SPF, PPRF, and ASCF is the developmental direction to achieve mechanical stability. How to gain biological fixation to improve the long-term stability is an urgent problem to be solved.
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http://dx.doi.org/10.7507/1002-1892.201712054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430014PMC
May 2018

The Regulation of Immunological Processes by Peripheral Neurons in Homeostasis and Disease.

Trends Immunol 2015 Oct;36(10):578-604

Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard University, Cambridge, MA 02139, USA. Electronic address:

The nervous system and the immune system are the principal sensory interfaces between the internal and external environment. They are responsible for recognizing, integrating, and responding to varied stimuli, and have the capacity to form memories of these encounters leading to learned or 'adaptive' future responses. We review current understanding of the cross-regulation between these systems. The autonomic and somatosensory nervous systems regulate both the development and deployment of immune cells, with broad functions that impact on hematopoiesis as well as on priming, migration, and cytokine production. In turn, specific immune cell subsets contribute to homeostatic neural circuits such as those controlling metabolism, hypertension, and the inflammatory reflex. We examine the contribution of the somatosensory system to autoimmune, autoinflammatory, allergic, and infectious processes in barrier tissues and, in this context, discuss opportunities for therapeutic manipulation of neuro-immune interactions.
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http://dx.doi.org/10.1016/j.it.2015.08.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592743PMC
October 2015

Extrinsic and intrinsic signals converge on the Runx1/CBFβ transcription factor for nonpeptidergic nociceptor maturation.

Elife 2015 Sep 29;4:e10874. Epub 2015 Sep 29.

Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United States.

The generation of diverse neuronal subtypes involves specification of neural progenitors and, subsequently, postmitotic neuronal differentiation, a relatively poorly understood process. Here, we describe a mechanism whereby the neurotrophic factor NGF and the transcription factor Runx1 coordinate postmitotic differentiation of nonpeptidergic nociceptors, a major nociceptor subtype. We show that the integrity of a Runx1/CBFβ holocomplex is crucial for NGF-dependent nonpeptidergic nociceptor maturation. NGF signals through the ERK/MAPK pathway to promote expression of Cbfb but not Runx1 prior to maturation of nonpeptidergic nociceptors. In contrast, transcriptional initiation of Runx1 in nonpeptidergic nociceptor precursors is dependent on the homeodomain transcription factor Islet1, which is largely dispensable for Cbfb expression. Thus, an NGF/TrkA-MAPK-CBFβ pathway converges with Islet1-Runx1 signaling to promote Runx1/CBFβ holocomplex formation and nonpeptidergic nociceptor maturation. Convergence of extrinsic and intrinsic signals to control heterodimeric transcription factor complex formation provides a robust mechanism for postmitotic neuronal subtype specification.
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http://dx.doi.org/10.7554/eLife.10874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657622PMC
September 2015

Comparison of myocardial transmural perfusion gradient by magnetic resonance imaging to fractional flow reserve in patients with suspected coronary artery disease.

Am J Cardiol 2015 May 18;115(10):1333-40. Epub 2015 Feb 18.

Department of Cardiology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, China. Electronic address:

The goal of this study was to evaluate the diagnostic accuracy of transmural perfusion gradient (TPG) and transmural perfusion gradient reserve (TPGR) with 3.0 T cardiac magnetic resonance (CMR) against invasively determined fractional flow reserve (FFR) to detect coronary artery stenosis. Quantitative analysis of myocardial perfusion with CMR to diagnosis coronary artery disease (CAD) has been widely accepted. However, traditional transmural myocardial perfusion analysis with CMR neglects that endocardium is more vulnerable to ischemia than epicardium. TPG and TPGR can take the inhomogenous perfusion impairment into account and be more sensitive and specific for diagnosis of CAD. In this study, 71 patients (57 men, age 60.1 ± 6.4 years) with known or suspected CAD referred for invasive angiography study underwent rest and adenosine-induced stress CMR perfusion imaging scan. FFR was attempted to be measured in all major epicardial coronary arteries. FFR ≤0.75 was regarded to indicate a hemodynamic significant coronary lesion. A TPG ≤0.85 predicted significant CAD with sensitivity and specificity of 74.55% and 83.65%, respectively. Sensitivity and specificity of TPGR ≤0.81 were 90.91% and 89.94%, respectively. Area under the receiver-operating curve to detect FFR ≤0.75 was 0.86 for TPG and 0.95 for TPGR. TPGR yielded significantly better sensitivity and specificity for diagnosis of CAD than traditional myocardial blood flow, myocardial perfusion reserve, and TPG (p < 0.0001). In conclusion, TPG and TPGR analyses with MRI are capable of detecting hemodynamic stenosis of coronary artery and superior to traditional myocardial perfusion analysis. Furthermore, TPGR appears to be superior to TPG in the diagnosis of coronary artery stenosis.
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http://dx.doi.org/10.1016/j.amjcard.2015.02.039DOI Listing
May 2015

Coronary veins determine the pattern of sympathetic innervation in the developing heart.

Development 2013 Apr 5;140(7):1475-85. Epub 2013 Mar 5.

Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10/6C103, 10 Center Drive, Bethesda, MD 20892, USA.

Anatomical congruence of peripheral nerves and blood vessels is well recognized in a variety of tissues. Their physical proximity and similar branching patterns suggest that the development of these networks might be a coordinated process. Here we show that large diameter coronary veins serve as an intermediate template for distal sympathetic axon extension in the subepicardial layer of the dorsal ventricular wall of the developing mouse heart. Vascular smooth muscle cells (VSMCs) associate with large diameter veins during angiogenesis. In vivo and in vitro experiments demonstrate that these cells mediate extension of sympathetic axons via nerve growth factor (NGF). This association enables topological targeting of axons to final targets such as large diameter coronary arteries in the deeper myocardial layer. As axons extend along veins, arterial VSMCs begin to secrete NGF, which allows axons to reach target cells. We propose a sequential mechanism in which initial axon extension in the subepicardium is governed by transient NGF expression by VSMCs as they are recruited to coronary veins; subsequently, VSMCs in the myocardium begin to express NGF as they are recruited by remodeling arteries, attracting axons toward their final targets. The proposed mechanism underlies a distinct, stereotypical pattern of autonomic innervation that is adapted to the complex tissue structure and physiology of the heart.
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http://dx.doi.org/10.1242/dev.087601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596991PMC
April 2013

Sexually dimorphic BDNF signaling directs sensory innervation of the mammary gland.

Science 2012 Dec;338(6112):1357-60

The Solomon H. Snyder Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

How neural circuits associated with sexually dimorphic organs are differentially assembled during development is unclear. Here, we report a sexually dimorphic pattern of mouse mammary gland sensory innervation and the mechanism of its formation. Brain-derived neurotrophic factor (BDNF), emanating from mammary mesenchyme and signaling through its receptor TrkB on sensory axons, is required for establishing mammary gland sensory innervation of both sexes at early developmental stages. Subsequently, in males, androgens promote mammary mesenchymal expression of a truncated form of TrkB, which prevents BDNF-TrkB signaling in sensory axons and leads to a rapid loss of mammary gland innervation independent of neuronal apoptosis. Thus, sex hormone regulation of a neurotrophic factor signal directs sexually dimorphic axonal growth and maintenance, resulting in generation of a sex-specific neural circuit.
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http://dx.doi.org/10.1126/science.1228258DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826154PMC
December 2012

PDZ domains of Par-3 as potential phosphoinositide signaling integrators.

Mol Cell 2007 Dec;28(5):886-98

Department of Biochemistry, Molecular Neuroscience Center, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P.R. China.

Multiple PDZ domain scaffold protein Par-3 and phosphoinositides (PIPs) are required for polarity in diverse cell types. We show that the second PDZ domain of Par-3 binds to phosphatidylinositol (PI) lipid membranes with high affinity. We further demonstrate that a large subset of PDZ domains in mammalian genomes are capable of binding to PI lipid membranes, indicating that lipid binding is the second most prevalent interaction mode of PDZ domains known to date. The biochemical and structural basis of Par-3 PDZ2-mediated membrane interaction is characterized in detail. The membrane binding capacity of Par-3 PDZ2 is critical for epithelial cell polarization. Interestingly, the lipid phosphatase PTEN directly binds to the third PDZ domain of Par-3. The concatenation of the PIP-binding PDZ2 and the lipid phosphatase PTEN-binding PDZ3 endows Par-3 as an ideal scaffold protein for integrating PIP signaling events during cellular polarization.
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http://dx.doi.org/10.1016/j.molcel.2007.10.028DOI Listing
December 2007
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