Publications by authors named "Sitong Wang"

16 Publications

  • Page 1 of 1

The significance of homeodomain transcription factor 2 in colon cancer cells.

Biomed Eng Online 2021 Aug 9;20(1):81. Epub 2021 Aug 9.

Department of Oncology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road Middle, Shanghai, 200072, People's Republic of China.

Background: Colon cancer is a serious malignant tumor. It has been reported that paired-like homeodomain transcription factor 2 (PITX2) can promote the progression of several types of cancer via regulating the Wnt/β-catenin pathway. It has also been demonstrated that high levels of long non-coding RNA (lncRNA) gastric carcinoma high expressed transcript 1 (GHET1) can also promote the development of cervical cancer via activating the Wnt/β-catenin pathway. However, whether PITX2 can affect the development of colon cancer via regulating the expression of lncRNA GHET1 remains unclear.

Results: The results demonstrated that PITX2 knockdown attenuated the proliferation, migration and invasion abilities of colon cancer cells. Additionally, PITX2 promoted the expression of lncRNA GHET1 via binding to its promoter. Overexpression of lncRNA GHET1 induced the expression of Wnt/β-catenin signaling-related proteins, cyclin D1, c-Myc and MMP-7. Furthermore, lncRNA GHET1 overexpression abrogated the PITX2 silencing-mediated decreased proliferation, migration and invasion abilities of colon cancer cells.

Conclusion: The findings of the present study suggested that PITX2 could enhance the proliferation, migration and invasion abilities of colon cancer cells via upregulating lncRNA GHET1 and activating the Wnt/β-catenin pathway.
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http://dx.doi.org/10.1186/s12938-021-00912-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351361PMC
August 2021

The upregulation of PYCR2 is associated with aggressive colon cancer progression and a poor prognosis.

Biochem Biophys Res Commun 2021 Oct 28;572:20-26. Epub 2021 Jul 28.

Department of Oncology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China; Tongji University Cancer Center, Shanghai, China. Electronic address:

PYCR2 has previously been shown to be related to a range of malignancies including hepatocellular carcinoma and melanoma, but its mechanistic functions and prognostic relevance in colon cancer patients remain to be defined. Herein, we used the Oncomine, Human Protein Atlas, The Cancer Genome Atlas (TCGA), and UALCAN databases to explore the expression of this gene in different human cancer, after which the relationship between PYCR2 expression and patient clinicopathologic characteristics was evaluated. We utilized an in vitro approach to evaluate the association between PYCR2 expression and colon cancer cell proliferation, migration, invasion, and tumor microsphere formation. The cell apoptosis was analyzed by flow cytometry. Gene set enrichment analysis (GSEA) approaches were additionally used to probe signaling pathways related to PYCR2. These analyses confirmed that PYCR2 was upregulated in several cancer types including colon cancer, with such upregulation correlating with a poor patient prognosis and with malignant clinicopathological characteristics. PYCR2 expression was identified as an independent predictor of colon cancer patients' survival, and in vitro analyses suggested that knocking down this gene was sufficient to disrupt the proliferative, migratory, invasive, and microsphere formation activities of colon cancer cells. Moreover, shPYCR2 transfection induced colon cancer cell apoptosis. GSEA suggested that high PYCR2 expression correlates with the differential enrichment of the Wnt β-catenin signaling, MYC targets, RNA polymerase, and Notch signaling pathways. Overall, these data indicate that PYCR2 is an important mediator of tumor progression and metastasis, and suggest that it may be a valuable prognostic indicator for colon cancer patient evaluation.
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http://dx.doi.org/10.1016/j.bbrc.2021.07.084DOI Listing
October 2021

"Shared Decision Making Assistant": A Smartphone Application to Meet the Decision-Making Needs of Patients With Primary Liver Cancer.

Comput Inform Nurs 2021 Jun 2. Epub 2021 Jun 2.

Author Affiliations: Department of Nursing (Drs Wang, Ye, Yunyun Li, and L. Li), Department of No. 3 Hepatobiliary Surgery (Dr Pan), Department of No. 5 Hepatobiliary Surgery (Dr Yang), and Department of Organ Transplantation (Dr Yu Li), Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, PR China.

The development of medical technology provides medical specialists with a variety of choices for their primary liver cancer patients, including partial liver resection, transcatheter arterial chemoembolization, liver transplantation, and so on. However, in this context, because patients with primary liver cancer frequently do not receive adequate information to help make complicated medical decisions, those patients, who are usually otherwise ignorant about their disease, are facing multiple difficult choices. The problem might be alleviated with a process called "shared decision making." Accordingly, researchers developed a smartphone application named "Shared Decision Making Assistant" for primary liver cancer patients in China, and in this article, we report the process of its development. First, individual interviews were conducted to identify the specific needs and status of primary liver cancer patients participating in shared decision making. Next, expert group discussions were held among primary liver cancer medical experts, nurses, and software engineers, using a decision-making process called the Delphi method, which was used to arrive at a group opinion or decision by surveying a panel of experts, to draft the framework and decide on the contents of the mobile health-based decision aids program. Feedbacks and suggestions were collected to optimize the workflow of "Shared Decision Making Assistant." The resulting application consisted of seven modules: personal information, primary liver cancer treatment knowledge center, decision aids path, continuing care, interactive platform, health education, and backstage management.
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http://dx.doi.org/10.1097/CIN.0000000000000775DOI Listing
June 2021

Margination and adhesion dynamics of tumor cells in a real microvascular network.

PLoS Comput Biol 2021 02 19;17(2):e1008746. Epub 2021 Feb 19.

Department of Computational Mathematics, School of Mathematics, Jilin University, Changchun, China.

In tumor metastasis, the margination and adhesion of tumor cells are two critical and closely related steps, which may determine the destination where the tumor cells extravasate to. We performed a direct three-dimensional simulation on the behaviors of the tumor cells in a real microvascular network, by a hybrid method of the smoothed dissipative particle dynamics and immersed boundary method (SDPD-IBM). The tumor cells are found to adhere at the microvascular bifurcations more frequently, and there is a positive correlation between the adhesion of the tumor cells and the wall-directed force from the surrounding red blood cells (RBCs). The larger the wall-directed force is, the closer the tumor cells are marginated towards the wall, and the higher the probability of adhesion behavior happen is. A relatively low or high hematocrit can help to prevent the adhesion of tumor cells, and similarly, increasing the shear rate of blood flow can serve the same purpose. These results suggest that the tumor cells may be more likely to extravasate at the microvascular bifurcations if the blood flow is slow and the hematocrit is moderate.
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http://dx.doi.org/10.1371/journal.pcbi.1008746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928530PMC
February 2021

Fast and large-range frequency hopping receiving based on simultaneous photonic filtering and digitizing.

Opt Lett 2021 Feb;46(4):749-752

We propose a frequency hopping receiving method that can receive signals with a large frequency hopping range and rapid hopping speed simultaneously. It is based on simultaneous photonic filtering and digitizing, and the reception frequency is tuned by changing the temporal shape of the sampling optical pulse. The proposed receiving method is verified by an experimental verification scheme. In the verification scheme, the optical pulse from a mode-locked laser is shaped to obtain several sub-pulses with different temporal shapes by spectral shaping and frequency-to-time mapping. The reception of the frequency hopping signal is performed by introducing certain delay through a switchable delay line to select different sub-pulses as the optical sampling pulse. The frequency hopping speed and range of the verification scheme are limited mainly by the speed of the optical switches and the bandwidth of electro-optic modulators, respectively. In experiments, with available devices, frequency hopping signals with a switching time of 250 ns and signals whose carrier frequency hops from 13.35 GHz to 39.30 GHz are successfully received.
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http://dx.doi.org/10.1364/OL.415003DOI Listing
February 2021

Biomechanics in thrombus formation from direct cellular simulations.

Phys Rev E 2020 Oct;102(4-1):042410

Department of Computational Mathematics, School of Mathematics, Jilin University, Changchun, 130012, China.

Numerically reproducing the process of thrombus formation is highly desired for understanding its mechanism but still remains challenging due to the polydisperse feature of blood components and their multiple biochemical or biomechanical behaviors involved. We numerically implemented a simplified version of the process from the perspective of biomechanics, using a mesoscale particle-based method, smoothed dissipative particle dynamics-immersed boundary method. This version covers the adhesion and aggregation of platelets (PLTs), the deformation and aggregation of red blood cells (RBCs), and the interaction between PLTs and RBCs, as well as the blockage of microvessels. Four critical factors that can affect thrombus formation were investigated: the velocity of blood flow, the adhesive ability of PLTs, the interaction strength between PLTs and RBCs, and the deformability of RBCs. Increasing the velocity of blood flow was found to be the most effective way to reduce the microvessel blockage, and reducing the adhesive ability of PLTs is also a direct and efficient way. However, decreasing the interaction strength between PLTs and RBCs sometimes does not alleviate thrombus formation, and similarly, increasing the deformability of RBCs does not have a significant improvement for the severely blocked microvessel. These results imply that maintaining high-rate blood flow plays a crucial role in the prevention and treatment of thrombosis, which is even more effective than antiplatelet or anticoagulant drugs. The drugs or treatments concentrating on reducing the PLT-RBC interaction or softening the RBCs may not have a significant effect on the thrombosis.
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http://dx.doi.org/10.1103/PhysRevE.102.042410DOI Listing
October 2020

A high-resolution map of reactive nitrogen inputs to China.

Sci Data 2020 11 11;7(1):379. Epub 2020 Nov 11.

College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, P.R. China.

To feed an increasingly affluent population, reactive nitrogen (Nr) inputs to China's lands and waters have substantially increased over the past century. Today, China's Nr emissions account for over one third of global total emissions, leading to serious environmental pollution and health damages. Quantifying the spatial variability of Nr inputs is crucial for the identification of intervention points to mitigate Nr pollution, which, however, is not well known. Here, we present a database describing Nr inputs to China for the year 2017 with a 1 km × 1 km resolution, considering land use and Nr sources, compiled by using the CHANS model. Results show that the North China Plain, the Sichuan Basin and the Middle-Lower Yangtze River Plain are hotspots of Nr inputs, where per hectare Nr input is an order of magnitude higher than that in other regions. Cropland and surface water bodies receive much higher Nr inputs than other land use types. This unique database will provide basic data for research on environmental health and global change modelling.
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http://dx.doi.org/10.1038/s41597-020-00718-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658216PMC
November 2020

Spatio-temporal distribution of six pollutants and potential sources in the Hexi Corridor, Northwest China.

Environ Monit Assess 2020 Sep 7;192(10):624. Epub 2020 Sep 7.

College of Atmospheric Sciences, Lanzhou University, Lanzhou, China.

Particulate matter (PM) concentrations are affected by anthropogenic emissions and sand transport jointly; however, the relative contributions from those two aspects are usually unknown. In our work, statistical analysis and back trajectories model were used to identify the dominant source in such area, by taking Yumen City as an example. We come to the conclusion that local emissions dominate the concentration of airborne pollutants, while sand transport plays a significant role on PM concentration. The conclusions were supported by the following results. (1) PM monthly mean concentrations at the two air quality stations, which are 70 km far away from each other, have the similar levels and variation trend; furthermore, a regression analysis of PM and PM daily concentrations between both stations indicated a significant correlation, suggesting that PM at both locations was influenced by the same emission sources; (2) statistical analysis results revealed that PM concentration has a positive correlation with wind speed, indicating the wind-blown dust and sand contribute mainly on PM concentration; (3) back-trajectory clustering analysis indicates that long-distance transport particulates from dust sources and their pathways had a significant impact on local PM concentrations.
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http://dx.doi.org/10.1007/s10661-020-08590-xDOI Listing
September 2020

Early-stage dysfunction of hippocampal theta and gamma oscillations and its modulation of neural network in a transgenic 5xFAD mouse model.

Neurobiol Aging 2020 10 12;94:121-129. Epub 2020 May 12.

College of Life Sciences, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin, PR China. Electronic address:

Alzheimer's disease (AD) is pathologically characterized by amyloid-β (Aβ) accumulation, which induces Aβ-dependent neuronal dysfunctions. We focused on the early-stage disease progression and examined the neuronal network functioning in the 5xFAD mice. The simultaneous intracranial recordings were obtained from the hippocampal perforant path (PP) and the dentate gyrus (DG). Concomitant to Aβ accumulation, theta power was strongly attenuated in the PP and DG regions of 5xFAD mice compared to those in nontransgenic littermates. For either theta rhythm or gamma oscillation, the phase synchronization on the PP-DG pathway was impaired, evidenced by decreased phase locking value and diminished coherency index. To alleviate the neural oscillatory deficits in early-stage AD, a neural modulation approach (rTMS) was used to activate gamma oscillations and strengthen the synchronicity of neuronal activity on the PP-DG pathway. In brief, there was a significant neuronal network dysfunction at an early-stage AD-like pathology, which preceded the onset of cognitive deficits and was likely driven by Aβ accumulation, suggesting that the neural oscillation analysis played an important role in early AD diagnosis.
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http://dx.doi.org/10.1016/j.neurobiolaging.2020.05.002DOI Listing
October 2020

High-speed and broadband digital receiver based on optical sampling pulse waveform matching.

Opt Lett 2020 Mar;45(6):1338-1341

In this Letter, we propose a high-speed, broadband photonic digital receiver that can realize the matched filtering of the digital signal through shaping the optical sampling pulse according to the specific waveform of the transmitted digital signal. The receiver's filtering response is matched with the spectrum of the digital signal's specific waveform, and the instantaneous signal-to-average-noise ratio of the filtered signal is maximized at the sampling points. The principle of proposed receiver is theoretically analyzed and experimentally verified. The weak digital signals with different signal-to-noise ratio are detected and correctly distinguished in the experiments.
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http://dx.doi.org/10.1364/OL.385657DOI Listing
March 2020

Targeting STAT3 enhances NDV-induced immunogenic cell death in prostate cancer cells.

J Cell Mol Med 2020 04 26;24(7):4286-4297. Epub 2020 Feb 26.

Department of Medical Oncology, Shanghai Tenths People's Hospital, School of Medicine, Tongji University, Shanghai, China.

Oncolytic Newcastle disease virus (NDV) induces immunogenic cell death (ICD), liberating danger-associated molecular patterns (DAMPs) that provokes defiance in neoplastic malignancy. The present study aims to investigate whether and how oncolytic NDV triggers ICD in prostate cancer cells. We show that NDV/FMW, an oncolytic NDV strain FMW, elicited the expression and release of several ICD markers, that is calreticulin (CRT), heat shock proteins (HSP70/90) and high-mobility group box 1 (HMGB1), in prostate cancer cells. Furthermore, pharmacological repression of apoptosis, necroptosis, autophagy or endoplasmic reticulum (ER) stress exerted diverse effects on the HMGB1 and HSP70/90 evacuation in NDV/FMW-infected prostate cancer cells. Moreover, ICD markers induced in prostate cancer cells upon NDV/FMW infection, were enhanced by either treatment with a STAT3 (signal transducer and activator of transcription 3) inhibitor or shRNA-mediated knockdown of STAT3. In nude mice bearing prostate cancer cell-derived tumours, the tumours injected with the supernatants of NDV/FMW-infected cells grew smaller than mock-treated tumours. These results indicate that oncolytic NDV provokes the expression of ICD makers in prostate cancer cells. Our data also suggest that a combination of inhibition of STAT3 with oncolytic NDV could boost NDV-based anti-tumour effects against prostate cancer.
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http://dx.doi.org/10.1111/jcmm.15089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7171322PMC
April 2020

Photonic compressive receiver for multiple microwave frequency measurement.

Opt Express 2019 Sep;27(18):25364-25374

We present a photonic compressive receiver, where the frequency information of the captured signal is directly mapped to the time intervals between compressed pulses for multiple microwave frequency measurement. The theoretical measurement error, multiple-frequency resolution and effective measurement range are derived. The effects of dispersion deviation and the electrical bandwidth are also discussed. The theoretical results are verified by the measured pulse waveforms and frequency-time mapping relationship. A photonic compressive receiver with an effective measurement range of 42 GHz, a multiple-frequency resolution of 1.2 GHz, a measurement accuracy of 88 MHz and a signal interception period of 27 ns is experimentally obtained.
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http://dx.doi.org/10.1364/OE.27.025364DOI Listing
September 2019

STAT3 Contributes To Oncolytic Newcastle Disease Virus-Induced Immunogenic Cell Death in Melanoma Cells.

Front Oncol 2019 29;9:436. Epub 2019 May 29.

Department of Medical Oncology, School of Medicine, Shanghai Tenths People's Hospital, Tongji University, Shanghai, China.

Oncolytic viruses (OVs) are emerging as potent inducers of immunogenic cell death (ICD), releasing danger-associated molecular patterns (DAMPs) that induce potent anticancer immunity. Oncolytic Newcastle disease virus (NDV) has been shown to educe ICD in both glioma and lung cancer cells. The objective of this study is to investigate whether oncolytic NDV induces ICD in melanoma cells and how it is regulated. Various time points were actuated to check the expression and release of ICD markers induced by NDV strain, NDV/FMW in melanoma cell lines. The expression and release of ICD markers induced by oncolytic NDV strain, NDV/FMW, in melanoma cell lines at various time points were determined. Surface-exposed calreticulin (CRT) was inspected by confocal imaging. The supernatants of NDV/FMW infected cells were collected and concentrated for the determination of ATP secretion by ELISA, HMGB1, and HSP70/90 expression by immunoblot (IB) analysis. Pharmacological inhibition of apoptosis, autophagy, necroptosis, ER Stress, and STAT3 (signal transducer and activator of transcription 3) was achieved by treatment with small molecule inhibitors. Melanoma cell lines stably depleted of STAT3 were established with lentiviral constructs. Supernatants from NDV-infected cells were intratumorally injected to mice bearing melanoma cells-derived tumors. Oncolytic NDV induced CRT exposure, the release of HMGB1 and HSP70/90 as well as secretion of ATP in melanoma cells. Inhibition of apoptosis, autophagy, necroptosis or ER stress attenuated NDV/FMW-induced release of HMGB1 and HSP70/90. Moreover, NDV/FMW-induced ICD markers in melanoma cells were also suppressed by either treatment with a STAT3 inhibitor or shRNA-mediated depletion of STAT3. Of translational importance, treatment of mice bearing melanoma cells-derived tumors with supernatants from NDV/FMW-infected cells significantly inhibited tumor growth. Our data authenticate that oncolytic NDV/FMW might be a potent inducer of ICD in melanoma cells, which is amalgamated with several forms of cell death. We also show that STAT3 plays a role in NDV/FMW-induced ICD in melanoma cells. Together, our data highlight oncolytic NDV as propitious for cancer therapeutics by stimulatingan anti-melanoma immune response.
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http://dx.doi.org/10.3389/fonc.2019.00436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548873PMC
May 2019

Large-area superelastic graphene aerogels based on a room-temperature reduction self-assembly strategy for sensing and particulate matter (PM and PM) capture.

Nanoscale 2019 May;11(21):10372-10380

School of Chemistry and Chemical Engineering, Beijing Institute of Technology, 100081, P. R. China.

Graphene aerogels are emerging low density and superelasticity macroscopic porous materials with various applications. However, it still remains a challenge to develop a versatile strategy under ambient conditions for fabricating large-area, high-performance graphene aerogels, which is crucial for their practical applications. Here, we report a novel room-temperature reduction self-assembly (RTRS) strategy to fabricate large-area graphene aerogels under ambient conditions. The strategy is based on using unique hydrazine hydrates as reducing agents to generate stable microbubbles beneficial for the formation of macroporous graphene hydrogels. Interestingly, the resultant hydrogel followed by a simple pre-freeze treatment can be naturally dried into graphene aerogels without noticeable volume shrinkage or structure cracking. Benefiting from the mild conditions, a large-area graphene aerogel with a diameter of up to 27 cm was prepared as an example. The as-formed aerogels exhibit a stable honeycomb-like coarse-pores structure, a low density of 3.6 mg cm-3 and superelasticity (rapidly recoverable from 95% compression) which are suitable for pressure/strain sensors. Moreover, the aerogel exhibits superior particulate matter adsorption efficiency (PM2.5: 93.7%, PM10: 96.2%) and good recycling ability. Importantly, the preparation process is cost-effective and easily scalable without the need for any special drying techniques and heating processes, which provides an ideal platform for mass production of graphene aerogels toward practical applications.
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http://dx.doi.org/10.1039/c9nr02071cDOI Listing
May 2019

Principle of integrated filtering and digitizing based on periodic signal multiplying.

Opt Lett 2019 Apr;44(7):1766-1769

We propose a generic integrated filtering and digitizing approach that can complete signal filtering and digitizing simultaneously based on periodic signal multiplying. The filtering response can be reconfigured flexibly by adjusting the temporal shape of the local periodic signal and/or the impulse response of photodetection. The principle and features of proposed structure are analyzed and experimentally verified in the electrical domain and the photonic domain. The system responses in line with the theoretical and simulation results are experimentally measured.
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http://dx.doi.org/10.1364/OL.44.001766DOI Listing
April 2019

Permutation Mutual Information: A Novel Approach for Measuring Neuronal Phase-Amplitude Coupling.

Brain Topogr 2018 03 5;31(2):186-201. Epub 2017 Oct 5.

College of Life Sciences and Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin, 300071, People's Republic of China.

Cross-frequency phase-amplitude coupling (PAC) in neuronal oscillations network plays an important functional role in large scale neuronal communication and neuronal encoding. In the present study, a novel approach named permutation mutual information (PMI) was applied in measuring PAC. It is derived from the permutation entropy based on the mutual information theory, by which the mutual information of permutations of two time series can be evaluated. In order to verify the ability of PMI, a numerical test was performed by using both simulation data and experimental data. The performances of PMI were compared with that of two well-known methods, which were the mean vector length (MVL) and the modulation index (MI). It was found that the performance of PMI was similar to that of MI when measuring PAC intensity, but the coupling sensitivity of PMI was the highest among all these three approaches. Moreover, there was the lowest sensitivity in the MVL measurement, suggesting that MVL was a more conservative approach in detecting the existence of PAC. In addition, an ROC analysis showed that PMI performed better in measuring PAC compared to that of others. Furthermore, the experimental data, obtained from rats' hippocampal CA3 regions, were analyzed by using the three approaches. The result was essentially in line with that of the simulation performances. In a word, the results suggest that PMI is a better choice for assessing PAC under the certain conditions.
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http://dx.doi.org/10.1007/s10548-017-0599-2DOI Listing
March 2018
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