Pusat Perubatan Universiti Kebangsaan Malaysia
Primary Affiliation: Pusat Perubatan Universiti Kebangsaan Malaysia - Malaysia
PubMed Central Citations
10PubMed Central Citations
BMC Complement Altern Med 2013 Sep 5;13:217. Epub 2013 Sep 5.
Department of Pharmacology, Faculty of Medicine, The National University of Malaysia (UKM), Jalan Raja Muda Abd Aziz, 50300, Kuala Lumpur, Malaysia.
Fathilah et al. BMC Complementary and Alternative Medicine 2013, 13:217 http://www.biomedcentral.com/1472-6882/13/217
BMC Complementary and Alternative Medicine
Labisia Pumila var. alata (LPva) has shown potential as an alternative to estrogen replacement therapy (ERT) in prevention of estrogen-deficient osteoporosis. In earlier studies using postmenopausal model, LPva was able to reverse the ovariectomy-induced changes in biochemical markers, bone calcium, bone histomorphometric parameters and biomechanical strength. The mechanism behind these protective effects is unclear but LPva may have regulated factors that regulate bone remodeling. The aim of this study is to determine the bone-protective mechanism of LPva by measuring the expressions of several factors involved in bone formative and resorptive activities namely Osteoprotegerin (OPG), Receptor Activator of Nuclear Factor kappa-B Ligand (RANKL), Macrophage-Colony Stimulating Factor (MCSF) and Bone Morphogenetic Protein-2 (BMP-2). Thirty-two female Wistar rats were randomly divided into four groups: Sham-operated (Sham), ovariectomized control (OVXC), ovariectomized with Labisia pumila var. alata (LPva) and ovariectomized with ERT (Premarin(R)) (ERT). The LPva and ERT were administered via daily oral gavages at doses of 17.5 mg/kg and 64.5 mug/kg, respectively. Following two months of treatment, the rats were euthanized and the gene expressions of BMP-2, OPG, RANKL and MCSF in the femoral bones were measured using a branch - DNA technique. The RANKL gene expression was increased while the OPG and BMP-2 gene expressions were reduced in the OVXC group compared to the SHAM group. There were no significant changes in the MCSF gene expressions among the groups. Treatment with either LPva or ERT was able to prevent these ovariectomy-induced changes in the gene expressions in ovariectomized rats with similar efficacy. LPva may protect bone against estrogen deficiency-induced changes by regulating the RANKL, OPG and BMP-2 gene expressions. (PDF) Labisia pumila regulates bone-related.... Available from: https://www.researchgate.net/publication/256450502_Labisia_pumila_regulates_bone-related_genes_expressions_in_postmenopausal_osteoporosis_model [accessed Jul 30 2018].
Fathilah SN, 2013, Branched DNA: A Novel Technique for Molecular Diagnostics in Bone Studies. Research Updates in Medical Sciences 1(1) : 27-29
Research Updates in Medical Sciences, RUMes
Recently, there are increasing applications of molecular techniques for molecular diagnostics in bone studies. Bone is a mineralized tissue containing an abundant matrix, which makes RNA isolation difficult. The most common technique used for molecular diagnosis in bone disease is reverse transcriptase–polymerase chain reaction (RT-PCR) sequence amplification assay. Currently, there is an in-creasing use of the branched chain DNA (bDNA) signal amplification technology. This article provides an overview of bDNA technolo-gy for molecular diagnostics in bone studies. Molecular diagnostic assays using bDNA technology for detection of gene expressions are sensitive, specific and reliable tools in the detection of genes involved in bone diseases such as osteoporosis. (PDF) Branched DNA: A Novel Technique for.... Available from: https://www.researchgate.net/publication/237073220_Branched_DNA_A_Novel_Technique_for_Molecular_Diagnostics [accessed Jul 30 2018].
Evid Based Complement Alternat Med 2012 9;2012:948080. Epub 2012 Sep 9.
Department of Pharmacology, Universiti Kebangsaan Malaysia Medical Center, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia ; Division of Pharmacology, Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
J Ethnopharmacol 2012 Jun 21;142(1):294-9. Epub 2012 Apr 21.
Department of Pharmacology, Faculty of Medicine, National University of Malaysia-Universiti Kebangsaan Malaysia, Jalan Raja Muda Abd Aziz, 50300 KL, Malaysia.
Article in Journal of ethnopharmacology 142(1):294-9 · April 2012
Journal of ethnopharmacology
Labisia pumila var. alata (LP) is a phytoestrogenic herb with potential as an alternative to Estrogen Replacement Therapy (ERT) in the treatment of postmenopausal osteoporosis. LP has been reported to produce similar effects to ERT on the bone markers, but could not match ERT in terms of maintaining the bone calcium in postmenopausal osteoporosis rat model. This study aimed to examine in detail the effects of LP on the bone of postmenopausal osteoporosis rat model using bone histomorphometry. Thirty two female rats were randomly divided into groups of: Sham operated (Sham), ovariectomized control (OVXC), ovariectomized with Labisia pumila var. alata (LP) and ovariectomized with ERT (Premarin®) (ERT). The LP and ERT were administered through the route of oral gavage daily at the dose of 17.5 mg/kg and 64.5 μg/kg respectively. Following 2 months of treatment, rats were euthanized and the left femurs were dissected out and prepared for bone histomorphometry. Histomorphometric analysis revealed osteoporotic changes for the ovariectomized rats. Supplementation of LP to ovariectomized rats could prevent these osteoporotic changes, as effective as ERT. This confirmed that LP has potential as an alternative to ERT for prevention of postmenopausal osteoporosis. Labisia pumila protects the bone of estrogen-deficient rat model: A histomorphometric study | Request PDF. Available from: https://www.researchgate.net/publication/224866425_Labisia_pumila_protects_the_bone_of_estrogen-deficient_rat_model_A_histomorphometric_study [accessed Jul 30 2018].