Publications by authors named "Sisi Liu"

134 Publications

Facile Fabrication of Ultrasensitive Honeycomb Nano-Mesh Ultraviolet Photodetectors Based on Self-Assembled Plasmonic Architectures.

ACS Appl Mater Interfaces 2021 Jul 21. Epub 2021 Jul 21.

Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, China.

The dilemma of harvesting fugacious photons by photoactive nanomaterials of limited absorption volume fundamentally hinders the photodetection at relatively lower light intensities. To address the insufficient light utilization efficiency, spatial light confinement becomes an effective and promising approach. High-performance ultraviolet (UV) photodetectors based on the self-assembled Au nanoparticle/ZnO honeycomb nano-mesh (Au NP/ZnO HN) are demonstrated through a facile solution-processed method on anodized aluminum oxide (AAO) membranes. The congregated geometry of the self-assembled ZnO HNs is well-defined by the AAO matrixes, which also effectively collects the transmitted light beams back to the photoactive layers. Benefiting from surface plasmon resonance, the enhanced absorption of the ZnO HNs is eventually obtained via the recursive light utilization between Au NPs and AAO matrixes as a function of AAO pore diameters (). With a systematic control of the photodetector configurations, an optimal performance is obtained with growth duration of the ZnO HNs for 40 min on the AAO substrates ( = 100 nm), and an excellent responsivity of 23.4 A/W is witnessed even under a relatively low light intensity of 0.4 mW/cm, providing a novel route to realize high-performance UV photodetection under low-power illumination.
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http://dx.doi.org/10.1021/acsami.1c08739DOI Listing
July 2021

The global profiling of alkaloids in Aconitum stapfianum and analysis of detoxification material basis against Fuzi.

J Chromatogr A 2021 Jun 28;1652:462362. Epub 2021 Jun 28.

School of Resources Environmental & Chemical Engineering, Nanchang University, Nanchang 330031, China; Center for Post-doctoral research, China Academy of Chinese Medical Sciences, Beijng 100700, China. Electronic address:

Aconitum alkaloids are versatile in chemical structures and are well known for their bioactivity and toxicity. Cases of analogs with closely similar structures or positional isomers are widespread in herbs of the Aconitum genus. It is still challenging to rapidly identify unknown compounds via mass spectrometry, especially positional isomers. Herein, to profile the alkaloids of Aconitum stapfianum that possess bioactivity against intoxication by the lateral root of Aconitum carmichaelii (Fuzi), a strategy was developed by carefully determining the fragmentation pathways of authentic standards. A series of rules was summarized and involved charge site effects, hydrogen bonding effects, competitive channels between charge-remote reactions and charge migration reactions, and fragment patterns exhibiting a "diamond shape". Accordingly, a total of 124 alkaloids in A. stapfianum were tentatively characterized, including 85 potential new compounds and 24 sets of isomers. On the other hand, to explore the material basis of detoxification, a chemical constituent comparison was made between A. stapfianum and Fuzi, and principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed to identify markers that were different between the two . In total, 25 characteristic markers were identified to discriminate between these two herbal medicines, of which 14 compounds were specific for A. stapfianum and most of them were characteristic for a para-substituted benzoic acid ester at C-14.
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http://dx.doi.org/10.1016/j.chroma.2021.462362DOI Listing
June 2021

LiS In Situ Grown on Three-Dimensional Porous Carbon Architecture with Electron/Ion Channels and Dual Active Sites as Cathodes of Li-S Batteries.

ACS Appl Mater Interfaces 2021 Jul 6;13(28):32968-32977. Epub 2021 Jul 6.

National Base for International Science & Technology Cooperation, National Local Joint Engineering Laboratory for Key Materials of New Energy Storage Battery, Hunan Province Key Laboratory of Electrochemical Energy Storage & Conversion, School of Chemistry, Xiangtan University, Xiangtan 411105, China.

LiS-based Li-S batteries are taken as promising energy storage systems due to the high theoretical specific capacity/energy density and nature of a matching Li-metal-free anode. However, the cyclic stability of the LiS-based Li-S battery is seriously prevented by the shuttle effect of lithium polysulfides (LiPSs). Meanwhile, due to the poor electrical conductivity of LiS, the Li-S battery displays slow reaction kinetics. In this work, we design 3D-porous carbon (PC) architecture as a host for inhabiting the LiPS shuttle based on physical capture. Furthermore, this porous carbon architecture is modified by introducing two kinds of heteroatoms (N and S) to form dual active sites (named as NSPC) for chemically binding LiPSs and accelerating their conversion. The polyvinyl pyrrolidone-coated LiSO·HO is embedded in the NSPC skeleton and further forms the LiS/NSPC cathode via a carbothermal reduction process. In consequence, the NSPC architecture possesses continuous electron/ion channels and abundant active sites, which are beneficial to the fast diffusion of Li and timely conversion of sulfur species. As a result, the as-prepared LiS/NSPC cathode exhibits a high initial discharge capacity of 690 mAh g at a high rate of 1C and keeps a capacity of 587 mAh g after 200 cycles with a good capacity retention rate of 85% and low fading rate of 0.075% per cycle. Therefore, this work offers a brand-new platform to understand the synergistic effects of promoting reaction kinetics for LiS-based Li-S batteries.
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http://dx.doi.org/10.1021/acsami.1c07198DOI Listing
July 2021

The genus : species concepts, biological functions, and secondary metabolites.

Crit Rev Microbiol 2021 Jul 2:1-30. Epub 2021 Jul 2.

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, and Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, School of Life Sciences, Yunnan Institute of Microbiology, Yunnan University, Kunming, China.

The genus has worldwide distribution with diverse host habitats and exhibits potential utilisation as biocontrol agent, bioreactor and antibiotic producer. In this review, we firstly comprehensively summarise the current taxonomic status of species, including their category names, morphological features, habitats, and multigene phylogenetic relationships. Some species possess vital biological functions and potential applications in medicine, agriculture, industry, and environmental protection. A total of 147 secondary metabolites have been reported so far from , among which 95 are novel. This paper serves to provide an overview of their diverse structures with chemical classification and biological activities. To date, 27 species of have been documented; however, only seven have been investigated for their secondary metabolites or biological functions. Our review is expected to draw more attention to this genus for providing a taxonomic reference, discovering extensive biological functions, and searching in-depth for new bioactive natural products.
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http://dx.doi.org/10.1080/1040841X.2021.1933898DOI Listing
July 2021

The Psychological and Quality of Life Impacts on Women in Hong Kong during the COVID-19 Pandemic.

Int J Environ Res Public Health 2021 Jun 23;18(13). Epub 2021 Jun 23.

School of Nursing, Tung Wah College, Hong Kong, China.

The coronavirus (COVID-19) pandemic has caused a global health crisis. The adverse impacts on Asian women, including those in Hong Kong, are substantial. This cross-sectional online study examined the impacts of COVID-19 on Hong Kong women, including psychological effects, self-belief in coping, and quality of life, and was conducted over 4 weeks from July to August 2020. Females aged over 18, living in Hong Kong, and that could read Chinese, were included. Among 417 participants, 50.8% were aged below 50, 66.7% were married, 57.1% were caregivers, 61.4% had a family income of
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http://dx.doi.org/10.3390/ijerph18136734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267857PMC
June 2021

Is partial ablation appropriate for benign thyroid nodules? A retrospective study with long-term follow-up after microwave ablation.

Int J Hyperthermia 2021 ;38(1):923-930

Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, P. R. China.

Objective: This retrospective study aimed to investigate the efficacy and safety of partial ablation (PA) for benign thyroid nodules (BTNs) using microwave ablation (MWA) in a long-term follow-up.

Materials And Methods: Between February 2015 and April 2019, 236 patients with 236 BTNs (maximum diameter ≥2 cm) treated with ultrasound-guided MWA were enrolled. Contrast-enhanced ultrasound (CEUS) was performed within 3 d after ablation to determine whether there was residual tissue according to which the patients were assigned PA or complete ablation (CA). The volume reduction ratio (VRR) and complications were evaluated during follow-up.

Results: Eighty-two patients were enrolled in the PA group, and 154 were enrolled in the CA group. Both groups achieved continuous reductions in nodule volume and increases in VRR within 2 years after ablation. Although the VRR of the PA group at 4 years was lower than that of the CA group (65.54 vs. 95.08%; <.05), PA still achieved 'technical efficacy' with a volume reduction of more than 50%. The complication and side effect rates between the two groups showed no significant difference (.05).

Conclusions: Both PA and CA were safe and effective in reducing the volumes of BTNs in the long-term follow-up. For nodules with a large initial volume and dangerous location, PA with a small amount of residual tissue may be acceptable.
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http://dx.doi.org/10.1080/02656736.2021.1936217DOI Listing
July 2021

Identification of Carbazole Alkaloid Derivatives with Acylhydrazone as Novel Anti-TMV Agents with the Guidance of a Digital Fluorescence Visual Screening.

J Agric Food Chem 2021 Jul 24;69(26):7458-7466. Epub 2021 Jun 24.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. China.

Difficulty in preventing crops from plant viruses urges to discover novel efficient antiviral chemicals, which is sped up by precise screening methods. Fluorescence-based methods have recently been applied as innovative and rapid tools for visually monitoring the replication of viruses and screening of antivirals, whereas the quantification of fluorescence signals mainly depends on manually calculating the fluorescent spots, which is time-consuming and imprecise. In the present work, the fluorescence spots were automatically identified, and the fluorescence area was directly quantified by a program developed in our group, which avoided subjective errors from the operators. We further employed this digital and visual screening assay to identify antivirals using the tobacco mosaic virus-green fluorescence protein (TMV-GFP) construct, in which the expression of GFP intuitively reflected the efficacy of antivirals. The accuracy of this assay was validated by quantifying the activities of the commercial antiviral inhibitors ribavirin and ningnanmycin and then was applied to evaluate the subtle activity differences of a series of newly synthesized carbazole and β-carboline alkaloid derivatives. Among them, compounds (76%) and (63%) exhibited anti-TMV activities comparable to that of ningnanmycin (65%) at 50 μM, and they delayed the multiplication of TMV in the early stage of infection without phytotoxicity. Taken together, these findings demonstrated that the digital and visual TMV-GFP screening method was competent to test the antiviral activities of compounds with subtle modifications and facilitated the discovery of novel antivirals.
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http://dx.doi.org/10.1021/acs.jafc.1c00897DOI Listing
July 2021

Self-Assembled Al Nanostructure/ZnO Quantum Dot Heterostructures for High Responsivity and Fast UV Photodetector.

Nanomicro Lett 2020 May 22;12(1):114. Epub 2020 May 22.

College of Electonics and Information, Kwangwoon University, Nowon-gu, Seoul, 01897, Republic of Korea.

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http://dx.doi.org/10.1007/s40820-020-00455-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770880PMC
May 2020

MicroRNA‑142‑3p suppresses cell proliferation, invasion and epithelial‑to‑mesenchymal transition via RAC1‑ERK1/2 signaling in colorectal cancer.

Mol Med Rep 2021 Aug 10;24(2). Epub 2021 Jun 10.

Department of Pathology, The First Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570102, P.R. China.

Aberrant expression of microRNAs (miRNAs/miRs) is associated with the initiation and progression of colorectal cancer (CRC), but how they regulate colorectal tumorigenesis is still unknown. The present study was designed to investigate the expression profile of miRNAs in human CRC tissues, and to reveal the molecular mechanism of miRNA‑142‑3p in suppressing colon cancer cell proliferation. The expression of miRNA was examined using an Exiqon miRNA array. Bioinformatics was used to predict the target genes of differentially expressed miRNAs and to analyze their biological function in CRC. The effect of miR‑142‑3p in colon cancer cells was evaluated using cell proliferation, colony formation and Transwell assays. Dual‑luciferase reporter gene assays were performed to investigate the association between miR‑142‑3p and Rac family small GTPase 1 (RAC1). The effect of miR‑142‑3p regulation on colon cancer proliferation was assessed through western blotting and quantitative polymerase chain reaction analysis. Compared with their expression in adjacent non‑cancer mucosal tissues, 76 miRNAs were upregulated and 102 miRNAs were downregulated in CRC. One of the most significantly and differentially regulated miRNAs was miR‑142‑3p, which was downregulated in 81.0% (51/63) of primary CRC tissues. After transfection of miR‑142‑3p mimics into colon cancer cells, proliferation and colony formation were decreased, and migration and invasion were markedly suppressed. was a possible target of miR‑142‑3p, which was confirmed by dual‑luciferase reporter assay. Transfection of miR‑142‑3p mimics decreased the levels of RAC1 and suppressed epithelial‑to‑mesenchymal transition in colon cancer cells. The phosphorylation of extraceullar signal‑regulated kinase (ERK) was decreased significantly by the inhibition of RAC1 or transfection of miR‑142‑3p mimics in colon cancer cells. In conclusion, aberrant miRNAs are implicated in CRC. Decreased expression of miR‑142‑3p may be associated with CRC tumorigenesis via Rac1‑ERK signaling.
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http://dx.doi.org/10.3892/mmr.2021.12207DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201444PMC
August 2021

Salting-out effect promoting highly efficient ambient ammonia synthesis.

Nat Commun 2021 May 27;12(1):3198. Epub 2021 May 27.

College of Energy, Key Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu Province, Soochow University, Suzhou, China.

The electroreduction of nitrogen to ammonia offers a promising alternative to the energy-intensive Haber-Bosch process. Unfortunately, the reaction suffers from low activity and selectivity, owing to competing hydrogen evolution and the poor accessibility of nitrogen to the electrocatalyst. Here, we report that deliberately triggering a salting-out effect in a highly concentrated electrolyte can simultaneously tackle the above challenges and achieve highly efficient ammonia synthesis. The solute ions exhibit strong affinity for the surrounding HO molecules, forming a hydration shell and limiting their efficacy as both proton sources and solvents. This not only effectively suppresses hydrogen evolution but also ensures considerable nitrogen flux at the reaction interface via heterogeneous nucleation of the precipitate, thus facilitating the subsequent reduction process in terms of both selectivity and activity. As expected, even when assembled with a metal-free electrocatalyst, a high Faradaic efficiency of 71 ± 1.9% is achieved with this proof-of-concept system.
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http://dx.doi.org/10.1038/s41467-021-23360-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160333PMC
May 2021

Sedimentary ancient DNA reveals a threat of warming-induced alpine habitat loss to Tibetan Plateau plant diversity.

Nat Commun 2021 05 20;12(1):2995. Epub 2021 May 20.

Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Polar Terrestrial Environmental Systems, Potsdam, Germany.

Studies along elevational gradients worldwide usually find the highest plant taxa richness in mid-elevation forest belts. Hence, an increase in upper elevation diversity is expected in the course of warming-related treeline rise. Here, we use a time-series approach to infer past taxa richness from sedimentary ancient DNA from the south-eastern Tibetan Plateau over the last ~18,000 years. We find the highest total plant taxa richness during the cool phase after glacier retreat when the area contained extensive and diverse alpine habitats (14-10 ka); followed by a decline when forests expanded during the warm early- to mid-Holocene (10-3.6 ka). Livestock grazing since 3.6 ka promoted plant taxa richness only weakly. Based on these inferred dependencies, our simulation yields a substantive decrease in plant taxa richness in response to warming-related alpine habitat loss over the next centuries. Accordingly, efforts of Tibetan biodiversity conservation should include conclusions from palaeoecological evidence.
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http://dx.doi.org/10.1038/s41467-021-22986-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137883PMC
May 2021

Insight into the mechanism of action of scoparone inhibiting egg development of Tetranychus cinnabarinus Boisduval.

Comp Biochem Physiol C Toxicol Pharmacol 2021 Aug 21;246:109055. Epub 2021 Apr 21.

Institute of Pesticide Science, College of Plant Protection, Southwest University, Chongqing 400715, PR China. Electronic address:

Investigating the mechanisms of action of natural bioactive products against pests is a vital strategy to develop novel promising biopesticides. Scoparone, isolated from Artemisia capillaris, exhibited potent oviposition inhibition activity against Tetranychus cinnabarinus (a crop-threatening mite pests with strong fecundity). To explore the underlying mechanism, the vitellogenin (Vg) protein content, and Vg gene expression of mites from three consecutive generations of G0 individuals exposed to scoparone were determined, revealing marked inhibition. This study is the first to explore the egg development defect behaviour of mite pests induced by scoparone. The egg-laying inhibition of mites by scoparone was significantly increased by 47.43% compared with that of the control when TcVg was silenced by RNA interference (RNAi), suggesting that egg-development inhibition of female T. cinnabarinus by scoparone was mediated by low Vg gene expression. Furthermore, scoparone bound to the Vg protein in vitro, and its K value was 218.9 μM, implying its potential function in inhibiting the egg development of mites by directly targeting the Vg protein. This study will lay the foundation for the future applications of scoparone as an agrochemical for controlling the strong egg-laying capacity mite pests in agriculture.
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http://dx.doi.org/10.1016/j.cbpc.2021.109055DOI Listing
August 2021

Secondary Metabolites of the Genus : Structures, Bioactivities and Biosynthesis.

Molecules 2021 Mar 26;26(7). Epub 2021 Mar 26.

Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, China.

are regarded as important sources for the generation of various bioactive secondary metabolites with rich chemical and bioactive diversities. falls under the rare actinomycete genus with the potential to produce antibiotics. In this review, all literatures were searched in the Web of Science, Google Scholar and PubMed up to March 2021. The keywords used in the search strategy were "", "secondary metabolite", "new or novel compound", "bioactivity", "biosynthetic pathway" and "derivatives". The objective in this review is to summarize the chemical structures and biological activities of secondary metabolites from the genus . A total of 159 compounds derived from 8 known and 18 unidentified species are summarized in this paper. These secondary metabolites are mainly categorized into polyphenols, linear polyketides, macrolides, macrolactams, thiazolyl peptides, cyclic peptides, glycopeptides, amide and amino derivatives, glycoside derivatives, enediyne derivatives and sesquiterpenes. Meanwhile, they mainly showed unique antimicrobial, anti-cancer, antioxidant, anti-hyperglycemic, and enzyme inhibition activities. In addition, the biosynthetic pathways of several potent bioactive compounds and derivatives are included and the prospect of the chemical substances obtained from is also discussed to provide ideas for their implementation in the field of therapeutics and drug discovery.
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http://dx.doi.org/10.3390/molecules26071884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037709PMC
March 2021

Sparse-view imaging of a fiber internal structure in holographic diffraction tomography via a convolutional neural network.

Appl Opt 2021 Feb;60(4):A234-A242

Deep learning has recently shown great potential in computational imaging. Here, we propose a deep-learning-based reconstruction method to realize the sparse-view imaging of a fiber internal structure in holographic diffraction tomography. By taking the sparse-view sinogram as the input and the cross-section image obtained by the dense-view sinogram as the ground truth, the neural network can reconstruct the cross-section image from the sparse-view sinogram. It performs better than the corresponding filtered back-projection algorithm with a sparse-view sinogram, both in the case of simulated data and real experimental data.
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http://dx.doi.org/10.1364/AO.404276DOI Listing
February 2021

Diffusion kurtosis imaging to evaluate the effect and mechanism of tetramethylpyrazine on cognitive impairment induced by lipopolysaccharide in rats.

Brain Imaging Behav 2021 Feb 11. Epub 2021 Feb 11.

Department of Radiology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong, 510630, China.

Using diffusion kurtosis imaging (DKI) to evaluate the brain changes, the therapeutic effect and mechanism of tetramethylpyrazine in rats with dementia induced by lipopolysaccharide. Thirty-six male Sprague-Dawley rats were randomly divided into control group and five groups pretreated with sham operation, lipopolysaccharide(150ug) and three doses of tetramethylpyrazine(5, 10, and 20 mg/mL respectively). The Morris water maze test was used to evaluate cognitive ability. DKI and histology were performed. Low-dose of tetramethylpyrazine pretreated rats showed lower escape latency(6th day: 15.92seconds(s) vs. 5.11 s, P = 0.001), spent more time in the target quadrant(15.67 s vs. 29.83 s, P = 0.009) and crossed the platform area more frequently(3.50 vs. 9.17, P = 0.001) than rats in the LPS-treated group. Compared to sham group, the fractional anisotropy (FA), axial diffusion (Da), mean kurtosis (MK), and axial kurtosis (Ka) values in the cortex of lipopolysaccharide group were lower (P = 0.021,0.003,0.003,0.001,respectively).The MK, Ka, Kr, and FA values in the hippocampus of the lipopolysaccharide group were higher (P = 0.01, 0.026,0.007,0.003,respectively),while MD and Da values were lower (P = 0.045,0.044, respectively). Tetramethylpyrazine-pretreated rats showed higher values of FA, MD, Da, MK, and Ka in the cortex, lower MK, Ka, Kr, and FA values and higher MD,Da values in the hippocampus than the lipopolysaccharide group. Histologically, prominent inflammatory cells infiltration in the brain parenchyma of lipopolysaccharide group were observed, while groups pretreated using tetramethylpyrazine were alleviated. Tetramethylpyrazine can improve cognitive dysfunction induced by lipopolysaccharide. DKI can sensitively detect microstructure integrity of brain parenchyma in a non-invasive manner.
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http://dx.doi.org/10.1007/s11682-021-00449-0DOI Listing
February 2021

Efficiently Passivated PbSe Quantum Dot Solids for Infrared Photovoltaics.

ACS Nano 2021 Feb 29;15(2):3376-3386. Epub 2021 Jan 29.

School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

Infrared (IR) solar cells are promising devices for significantly improving the power conversion efficiency of common solar cells by harvesting the low-energy IR photons. PbSe quantum dots (QDs) are superior IR photon absorbing materials due to their strong quantum confinement and thus strong interdot electronic coupling. However, the high chemical activity of PbSe QDs leads to etching and poor passivation in ligand exchange, resulting in a high trap-state density and a high open circuit voltage () deficit. Here we develop a hybrid ligand co-passivation strategy to simultaneously passivate the Pb and Se sites; that is, halide anions passivate the Pb sites and Cd cations passivate the Se sites. The cation and anion hybrid passivation substantially improves the quality of PbSe QD solids, giving rise to an excellent trap-state control and prolonged carrier lifetime. A high and a high short circuit current density () are achieved simultaneously in the IR QD solar cells based on this hybrid ligand treatment. Finally, a IR-PCE of 1.31% under the 1100-nm-filtered solar illumination is achieved in the PbSe QD solar cells, which is the highest IR-PCE for PbSe QD IR solar cells at present. Additionally, the PbSe QD devices show a high external quantum efficiency of 80% at ∼1295 nm.
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http://dx.doi.org/10.1021/acsnano.0c10373DOI Listing
February 2021

Analysis of synonymous codon usage of transcriptome database in .

PeerJ 2021 4;9:e10450. Epub 2021 Jan 4.

College of Traditional Chinese Medicine, Hebei University, Baoding, China.

Background: is an endangered and important medicinal plant in Asian countries, especially in China. However, there is little knowledge about the codon usage bias for CDSs. In this project, codon usage bias was determined based on the 2,626 predicted CDSs from R. palmatum transcriptome.

Methods: In this study, all codon usage bias parameters and nucleotide compositions were calculated by Python script, Codon W, DNA Star, CUSP of EMBOSS.

Results: The average GC and GC3 content are 46.57% and 46.6%, respectively, the results suggested that there exists a little more AT than GC in the genes, and the codon bias of genes preferred to end with A/T. We concluded that the codon bias in was affect by nucleotide composition, mutation pressure, natural selection, gene expression levels, and the mutation pressure is the prominent factor. In addition, we figured out 28 optimal codons and most of them ended with A or U. The project here can offer important information for further studies on enhancing the gene expression using codon optimization in heterogeneous expression system, predicting the genetic and evolutionary mechanisms in .
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http://dx.doi.org/10.7717/peerj.10450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789865PMC
January 2021

Corrigendum to Matrix stiffening induces endothelial dysfunction via the TRPV4/microRNA-6740/endothelin-1 mechanotransduction pathway Acta Biomaterialia, Volume 100, December 2019, Pages 52-60.

Acta Biomater 2021 Mar 23;122:387-388. Epub 2021 Jan 23.

Institute of Health Service and Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100039, P.R.China.

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http://dx.doi.org/10.1016/j.actbio.2021.01.009DOI Listing
March 2021

Geometric Confinement Guides the Expression of Cancer Stem Cell Molecular Markers CD44 via Cell Traction Forces.

ACS Biomater Sci Eng 2020 08 30;6(8):4623-4630. Epub 2020 Jul 30.

Institute of Biomechanics and Medical Engineering, Department of Engineering Mechanics, Tsinghua University, Beijing 100084, P. R. China.

Cancer stem cells (CSCs) play a critical role in the cancer metastasis and account for tumor heterogeneity. Growing evidence indicates that the CSC phenotypes are related to the tumor microenvironment. In this study, we report that the gradient of mechanical stresses guides the spatial patterning of the expression of CD44 and Yes-associated protein (YAP) in the geometrically confined multicellular sheets. Our study shows that the cytoskeletal contraction regulates the expression of CD44 through the translocation of YAP into the nucleus. The results demonstrate that geometric confinement and mechanical stresses are the regulators in the spatial patterning of CSC. It may help to understand the relationship between the tumor microenvironment and oncogenesis.
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http://dx.doi.org/10.1021/acsbiomaterials.0c00366DOI Listing
August 2020

A novel somatic BRCA2 point mutation in a metastatic pancreatic cancer patient: a case report.

BMC Med Genomics 2021 01 6;14(1). Epub 2021 Jan 6.

Department of Medical Oncology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China.

Background: In addition to ovarian and breast cancers, loss-of-function mutations in BRCA1 and BRCA2 genes are also linked to an increased risk of pancreatic cancer, with ~ 4 to 7% of pancreatic cancer patients harboring germline BRCA mutations. Most BRCA alterations in pancreatic cancer are frame-shifting indels, stop-gain, and splice-site mutations, but single nucleotide substitutions are rare. Recent studies demonstrated a significant progression-free survival (PFS) benefit from maintenance olaparib, a poly (ADP-ribose) polymerase (PARP) inhibitor administered to patients with germline BRCA mutations and metastatic pancreatic cancer.

Case Presentation: Here, we report a metastatic pancreatic cancer case who harbored a novel somatic BRCA2 c.6944T > C (p. I2315T) point mutation. After 6 weeks first-line chemotherapy, the patient was refractory to treatment and had a progressive disease. Due to the novel nonsynonymous BRCA2 point mutation, we decided to change the strategy by administering olaparib. The patient benefited from olaparib therapy and achieved a PFS of ~ 6.5 months.

Conclusions: We describe a patient carrying a novel somatic BRCA2 p. I2315T point mutation, which is first reported in metastatic pancreatic cancer. This case report indicates that a gene mutation-based strategy should be considered in the clinic to provide more effective treatment.
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http://dx.doi.org/10.1186/s12920-020-00850-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788890PMC
January 2021

Genomic and clinical characteristics of exon14 alterations in a large cohort of Chinese cancer patients revealed distinct features and a novel resistance mechanism for crizotinib.

J Cancer 2021 1;12(3):644-651. Epub 2021 Jan 1.

Department of Medical Oncology, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, China.

Alterations in exon 14 (ex14) and its flanking intronic regions have been identified in a variety of cancers. Patients with ex14 alterations often benefit from MET inhibitors such as crizotinib. Given the unique mutation profiles of Chinese lung cancer patients, it is necessary to investigate the prevalence of ex14 alterations in a large cohort of cancer patients. Cases carrying ex14 alterations were screened from 26,391 Chinese cancer patients by next-generation sequencing (NGS), and the clinicopathologic and molecular characteristics were reviewed. Compared to Western population (~3%), the frequency of ex14 alterations is much lower in Chinese cancer patients (0.7%, n=184) and lung cancer patients (1.1%, n=175). Seventy-eight distinct ex14 alterations, including several novel alteration types were detected. Concurrent copy gain and non-exon14 mutations were also found. copy gain (11%) and mutations (8%), (5%) and (5%), appeared in a mutually exclusive pattern. Female patients contain much less mutations than male patients (65% vs. 24%, FDR = 0.01). Co-amplification of and , and were identified, which indicated cell cycle dysregulation and alteration are important co-occurring features in patients with ex 14 alteration. Of 9 tissue specimens having PD-L1 immunohistochemistry (IHC) results, 5 of them (55.5%) were found PD-L1 positive, which is comparable to other types of tumor. In 14 crizotinib-treated patients, the median progression free survival (mPFS) was 7 months. Upon resistance to crizotinib, two patients acquired secondary mutations in and one patient acquired p.K601E that can be a novel resistance mechanism. Chinese cancer patients have a relatively lower frequency of ex14 alterations compared to Western patients. Patients with ex14 alterations showed distinct molecular characteristics and the representative case study showed responses to MET tyrosine kinase inhibitor (TKI).
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http://dx.doi.org/10.7150/jca.49391DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778531PMC
January 2021

Plant diversity in sedimentary DNA obtained from high-latitude (Siberia) and high-elevation lakes (China).

Biodivers Data J 2020 14;8:e57089. Epub 2020 Dec 14.

Institute of Environmental Science and Geography, University of Potsdam, Potsdam, Germany Institute of Environmental Science and Geography, University of Potsdam Potsdam Germany.

Background: Plant diversity in the Arctic and at high altitudes strongly depends on and rebounds to climatic and environmental variability and is nowadays tremendously impacted by recent climate warming. Therefore, past changes in plant diversity in the high Arctic and high-altitude regions are used to infer climatic and environmental changes through time and allow future predictions. Sedimentary DNA (sedDNA) is an established proxy for the detection of local plant diversity in lake sediments, but still relationships between environmental conditions and preservation of the plant sedDNA proxy are far from being fully understood. Studying modern relationships between environmental conditions and plant sedDNA will improve our understanding under which conditions sedDNA is well-preserved helping to a.) evaluate suitable localities for sedDNA approaches, b.) provide analogues for preservation conditions and c.) conduct reconstruction of plant diversity and climate change. This study investigates modern plant diversity applying a plant-specific metabarcoding approach on sedimentary DNA of surface sediment samples from 262 lake localities covering a large geographical, climatic and ecological gradient. Latitude ranges between 25°N and 73°N and longitude between 81°E and 161°E, including lowland lakes and elevated lakes up to 5168 m a.s.l. Further, our sampling localities cover a climatic gradient ranging in mean annual temperature between -15°C and +18°C and in mean annual precipitation between 36- and 935 mm. The localities in Siberia span over a large vegetational gradient including tundra, open woodland and boreal forest. Lake localities in China include alpine meadow, shrub, forest and steppe and also cultivated areas. The assessment of plant diversity in the underlying dataset was conducted by a specific plant metabarcoding approach.

New Information: We provide a large dataset of genetic plant diversity retrieved from surface sedimentary DNA from lakes in Siberia and China spanning over a large environmental gradient. Our dataset encompasses sedDNA sequence data of 259 surface lake sediments and three soil samples originating from Siberian and Chinese lakes. We used the established chloroplastidal P6 loop trnL marker for plant diversity assessment. The merged, filtered and assigned dataset includes 15,692,944 read counts resulting in 623 unique plant DNA sequence types which have a 100% match to either the EMBL or to the specific Arctic plant reference database. The underlying dataset includes a taxonomic list of identified plants and results from PCR replicates, as well as extraction blanks (BLANKs) and PCR negative controls (NTCs), which were run along with the investigated lake samples. This collection of plant metabarcoding data from modern lake sediments is still ongoing and additional data will be released in the future.
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http://dx.doi.org/10.3897/BDJ.8.e57089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7752886PMC
December 2020

Fusion in Lung Adenocarcinoma with Excellent Response Upon Alectinib Treatment: A Case Report and Literature Review.

Onco Targets Ther 2020 4;13:12515-12519. Epub 2020 Dec 4.

Department of Respiratory Oncology, Guangxi Medical University Affiliated Tumor Hospital, Nanning, China.

Non-small cell lung cancer (NSCLC) patients with anaplastic lymphoma kinase () rearrangement benefit from treatment with ALK inhibitors. Therefore, the identification of druggable fusions is necessary for NSCLC treatment. More than 90 fusion partners of have been reported in NSCLC patients, but the striatin gene () fusion has rarely been reported. Moreover, the response of - fusion patients treated with ALK inhibitors remains to be explored. A 64-year-old Chinese male with no history of smoking or alcohol consumption was diagnosed as stage IVB lung adenocarcinoma (LADC) (cT4N3M1c) in October 2018. Next-generation sequencing (NGS) targeting 425 cancer-related genes was performed on the plasma and supernatant of pleural effusion samples and revealed an - fusion. The patient received alectinib (600 mg, twice daily) as the first-line treatment with an excellent response exceeding 19 months. This is the first report of a NSCLC patient harboring an - fusion and exhibiting an excellent response to alectinib treatment. This case provides valuable information for therapeutic decision-making of patients with - fusions. Furthermore, this case also highlighted the advantage of performing targeted NGS on circulating tumor DNA for the identification and analysis of rare, druggable genomic alterations.
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http://dx.doi.org/10.2147/OTT.S282933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727031PMC
December 2020

Antitumor Efficacy of Oncolytic Herpes Virus Type 1 Armed with GM-CSF in Murine Uveal Melanoma Xenografts.

Cancer Manag Res 2020 18;12:11803-11812. Epub 2020 Nov 18.

Brain Tumor Research Center, Beijing Neurosurgical Institute, Beijing Laboratory of Biomedical Materials, Beijing Tiantan Hospital Affiliated to Capital Medical University, Beijing 100070, People's Republic of China.

Background: Uveal melanoma (UM) is the most common primary intraocular tumor in adults with a high incidence of metastasis. Standard care therapies for UM include enucleation and radiation, which are minimally effective in prolonging patient survival. Oncolytic virus treatment has become a new trend in cancer field. Of which, oncolytic herpes simplex virus type 1 (HSV-1) therapy is one of the most effective antitumor treatments. Here, we established an oncolytic HSV-1 encoding granulocyte-macrophage colony-stimulating factor (GM-CSF), tested its efficacy in UM therapy, and investigated the innate immune response induced by this virus.

Methods: Oncolytic HSV-1 expressing GM-CSF (HSV-GM-CSF) was constructed, then verified using qPCR and Western blot assays. Cell viability assays and transmission electron microscopy were conducted on three UM cell lines, MUM2B, 92.1, and MP41, to assess the cell-killing ability and virus infection of this virus. For in vivo experiments, BALB/c-nude mice in situ UM xenografts were established to testify the efficacy of the oncolytic virus, oncolytic HSV-1, and HSV-GM-CSF groups, respectively. IVIS images, ocular volumes, mice weights, and survivals were tracked to see the efficacy of the virus. Hematoxylin and eosin staining, immunohistochemistry, and flow cytometry analyses were conducted to demonstrate the immune activity after virus treatment.

Results: All three tested UM cell lines were sensitive to infection by HSV-GM-CSF. In vivo xenograft experiments revealed that oncolytic virus HSV-1 reduced UM tumor volume and that oncolytic virus HSV-1 armed with GM-CSF enhanced the antitumor effect compared with unmodified HSV-1. The bodyweights of untreated control group mice were significantly lower than those of mice in either virus-treated group (HSV-1 or HSV-GM-CSF). Follow-up survivals were prolonged in the virus-treated groups compared with the control group and were prolonged to a greater extent in the HSV-GM-CSF group than in the HSV-1 group. Macrophage stimulation was observed following HSV-GM-CSF treatment.

Conclusion: Our results indicate that the recombinant oncolytic virus HSV-GM-CSF is a potential therapeutic treatment for UM.
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http://dx.doi.org/10.2147/CMAR.S274605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680789PMC
November 2020

Piezo1 regulates migration and invasion of breast cancer cells via modulating cell mechanobiological properties.

Acta Biochim Biophys Sin (Shanghai) 2021 Jan;53(1):10-18

Institute of Biomechanics and Medical Engineering, School of Aerospace Engineering, Tsinghua University, Beijing 100084, China.

Cell migration and invasion are two essential processes during cancer metastasis. Increasing evidence has shown that the Piezo1 channel is involved in mediating cell migration and invasion in some types of cancers. However, the role of Piezo1 in the breast cancer and its underlying mechanisms have not been clarified yet. Here, we show that Piezo1 is high-expressed in breast cancer cell (BCC) lines, despite its complex expression in clinical patient database. Piezo1 knockdown (Piezo1-KD) promotes unconfined BCC migration, but impedes confined cell migration. Piezo1 may mediate BCC migration through the balances of cell adhesion, cell stiffness, and contractility. Furthermore, Piezo1-KD inhibits BCC invasion by impairing the invadopodium formation and suppressing the expression of metalloproteinases (MMPs) as well. However, the proliferation and cell cycle of BCCs are not significantly affected by Piezo1. Our study highlights a crucial role of Piezo1 in regulating migration and invasion of BCCs, indicating Piezo1 channel might be a new prognostic and therapeutic target in BCCs.
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http://dx.doi.org/10.1093/abbs/gmaa112DOI Listing
January 2021

Molecular diagnosis and clinical outcome of a lung cancer patient with -E285K mutated Li-Fraumeni syndrome harboring a somatic -KDD mutation.

Am J Transl Res 2020 15;12(10):6689-6693. Epub 2020 Oct 15.

The Second Department of Thoracic Medical Oncology, The Second Hospital of Dalian Medical University Dalian, Liaoning, China.

Objectives: Li-Fraumeni syndrome (LFS) is an autosomal dominant cancer predisposition, mostly caused by germline mutations. Lung adenocarcinoma (ADC) has been identified as the most frequent LFS-related cancer outside the common LFS core spectrum. EGFR-kinase domain duplication (KDD) is rare in lung cancer and the effective therapy for LFS patients with -KDD mutated ADC is unclear. This study reports the first case of a -mutated LFS patient with confirmed family history, developing advanced lung ADC harboring -KDD.

Materials And Methods: The patient's lung tumor, lymph nodes, liquid biopsies and germline control sample at various disease stages were subjected to next-generation sequencing (NGS). The germline mutation was confirmed using the peripheral blood of the patient's relatives by Sanger sequencing.

Results: A rare -KDD somatic mutation that was missed in the routine hotspots test, and a -E285K temperature-sensitive germline mutation were identified by NGS. The patient was diagnosed with breast cancer in 2006 and her family cancer history review revealed that seven out of 13 relatives were diagnosed or died from LFS-spectrum cancers before the age of 45 years. Three of the six relatives were positive for the -E285K germline mutation. This patient received multi-line chemotherapy followed by anlotinib, a multi-target tyrosine kinase inhibitor, upon the identification of -KDD, and achieved an overall survival of 18 months.

Conclusions: Our study highlights the importance of NGS in discovering rare genetic alterations to guide treatment decision-making, and provides meaningful insight into the potential treatment options for LFS patients with -KDD mutations.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653629PMC
October 2020

Efficacy of a novel double-controlled oncolytic adenovirus driven by the Ki67 core promoter and armed with IL-15 against glioblastoma cells.

Cell Biosci 2020 27;10:124. Epub 2020 Oct 27.

Brain Tumor Research Center, Beijing Neurosurgical Institute, Capital Medical University, Beijing, 100070 P.R. China.

Background: Glioblastoma (GBM) is an immunosuppressive, highly vascular and devastating malignant brain tumor. Even with progressive combination treatment that includes surgery, radiotherapy, and chemotherapy, the prognosis for GBM patients is still extremely poor. Oncolytic adenovirus (OAd) can specifically replicate in GBM cells, permitting the rapid copy of the therapeutic genes it carries. Moreover, E1A is an essential gene in adenoviral replication and is the first gene expressed upon viral infection. E1A expression can be regulated by the Ki67 promoter, while the CMV promoter drives therapeutic gene expression. However, the efficacy of a double-controlled OAd driven by the Ki67 core promoter and armed with IL-15 against GBM cells has not been investigated.

Methods: Fluorescence microscopy was performed to evaluate infection ability in the viruses. Cell viability was detected by CCK-8 assay. Levels of cytokines in different supernatants were determined by ELISA, and IL-15 gene expression was measured by RT-PCR. Angiogenic capacity was analyzed by tube formation assay.

Results: We successfully constructed a double-controlled oncolytic adenovirus driven by the Ki67 core promoter and armed with IL-15 that selectively infected and killed GBM cells while sparing normal cells. The adenoviruses prime IL-15 gene expression to significantly enhance anti-GBM efficacy through effective activation of microglial cells. Moreover, OAd not only directly inhibits angiogenesis but exhibits potent antiangiogenic capacity mediated by the reduction of VEGF secretion.

Conclusions: These results provide new insight into the effects of a novel double-controlled OAd driven by the Ki67 core promoter and armed with IL-15 in glioblastoma treatment, which may help in the development of novel therapies in solid tumors.
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http://dx.doi.org/10.1186/s13578-020-00485-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592588PMC
October 2020

Enhanced Spatial Light Confinement of All Inorganic Perovskite Photodetectors Based on Hybrid Plasmonic Nanostructures.

Small 2020 Nov 26;16(46):e2004234. Epub 2020 Oct 26.

Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, Sichuan, 610054, China.

3D incident light confinement by radical electromagnetic fields offers a facile and novel way to break through the performance limit of inorganic perovskite CsPbBr quantum dots (QDs). Herein, metallic nanoparticles decorated anodic aluminum oxide (AAO) hybrid plasmonic nanostructures with geometric control are first proposed for cyclic light utilization of perovskite photodetectors, enabled by spatially extended light confinement. The drastic multiple interference induced by plasmonic coupling within AAO matrixes are generated as a function of pore sizes, which can effectively collect the transmitted photons back to the surface. In addition, the self-assembled metallic nanoparticles simultaneously concentrate the incident and reflected light beams into the CsPbBr QD layers. The light confinement inherently stems from the metallic nanoparticles due to the variation of the near surface electromagnetic fields. As a result, perovskite photodetectors based on Al nanoparticles/AAO hybrid plasmonic nanostructures with a pore size of 220 nm exhibit enhanced photoresponse behavior with remarkably increased photocurrent by ≈43× and maintain low dark current under 490 nm light illumination at 1 V.
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http://dx.doi.org/10.1002/smll.202004234DOI Listing
November 2020

Macrophage polarization contributes to the efficacy of an oncolytic HSV-1 targeting human uveal melanoma in a murine xenograft model.

Exp Eye Res 2021 01 9;202:108285. Epub 2020 Oct 9.

Brain Tumor Research Center, Beijing Neurosurgical Institute, Department of Neurosurgery, Beijing Tiantan Hospital Affiliated to Capital Medical University, Beijing Laboratory of Biomedical Materials, Beijing, China. Electronic address:

Uveal melanoma (UM), the most common primary malignant tumor of the eye in adults, is difficult-to-treat. UM has a relatively high mortality secondary to distant metastasis and poor overall survival with existing therapies. The oncolytic virus herpes simplex virus type-1 (HSV-1) has been approved for clinical use in melanoma. This double-stranded DNA virus was suspected to directly activate lysis specifically in neoplastic cells. We tested the antitumor efficacy of recombinant oncolytic HSV-1-EGFP (oHSV-EGFP) in UM and characterized the local and systemic antitumor innate immune response in a murine xenograft model. We first determined the efficacy of the oncolytic virus in 92.1, MUM2B and MP41 cell lines. In murine xenograft models, oHSV-EGFP reduced intraocular tumors as well as systemic subcutaneous tumors. A significant change in cytokines was observed in viral infected cells, especially a rise in IFNγ. In vivo analyses showed increased anti-tumorigenic M1 macrophages and decreased pro-tumorigenic M2 macrophages in peripheral blood, and intraocular and distant tumors after intravitreal viral treatment. Increased infiltration of natural killer cells and mature dendritic cells was also detected after viral treatment. In addition, no virus was detected in major organs after the treatment. Our data support that oHSV-EGFP is effective, neoplasm specific, immune active and safe, providing possible clinical translatable options to treat ocular and metastatic UM.
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http://dx.doi.org/10.1016/j.exer.2020.108285DOI Listing
January 2021

Antitumor efficacy of oncolytic HSV-1 expressing cytosine deaminase is synergistically enhanced by DPD down-regulation and EMT inhibition in uveal melanoma xenograft.

Cancer Lett 2020 12 15;495:123-134. Epub 2020 Sep 15.

Brain Tumor Research Center, Beijing Neurosurgical Institute, Department of Neurosurgery, Beijing Tiantan Hospital Affiliated to Capital Medical University, Beijing Laboratory of Biomedical Materials, Beijing, China. Electronic address:

Uveal melanoma (UM) is the most common intraocular tumor in adults and has a high incidence of metastases. Possible treatments remain limited in UM with enucleation and radiation, leading to poor prognosis in this chemo-resistant carcinoma. Thus, urging demand for novel treatment is needed. We examined the antitumor efficacy of a new recombinant oncolytic herpes simplex virus type 1 (oHSV-1) armed with E.coli cytosine deaminase (CD). We determined the efficacy of the oncolytic virus in UM cell lines. In vivo experiments showed that oHSV-CD/5-fluorocytosine (5-FC) treatment reduce tumor volume and prolonged survival. We further demonstrated the molecular mechanisms of oHSV-CD/5-FC treatment. The oncolytic virus down-regulated IL-6 expression and thereby reversed the epithelial-mesenchymal transition (EMT) phenotype. Dihydropyrimidine dehydrogenase (DPD), the rate-limiting enzyme in 5-fluorouracil (5-FU) metabolism, was also down-regulated. Therefore, the efficacy of oHSV-CD/5-FC was synergistically enhanced by DPD down-regulation and EMT inhibition. This study provides solid evidence for the antitumor efficacy of oHSV-CD/5-FC treatment in vitro and in vivo. The molecular mechanisms of this treatment may bring a new therapeutic approach for future treatment of UM.
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http://dx.doi.org/10.1016/j.canlet.2020.09.013DOI Listing
December 2020