Publications by authors named "Sinan Chen"

14 Publications

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Ultrasound-assisted ethanol extraction of Actinidia arguta pollen possesses antioxidant activity and protects DNA from oxidative damage.

J Food Biochem 2021 04 22;45(4):e13603. Epub 2021 Feb 22.

College of Food Science and Technology, Northwest University, Xi'an, China.

Actinidia arguta pollen owns abundant nutrients, such as vitamins, polyphenols, etc., however, little research on its antioxidant ability and biological function was conducted. In this study, we observed A. arguta pollen spore structure by SEM (Scanning electron microscope), analyzed the phenolic composition of A. arguta pollen extract (AAPE) obtained by four extraction methods (A: ultrasound-assisted extraction with water, B: heat reflux extraction with water, C: ultrasound-assisted extraction with ethanol, and D: heat reflux extraction with ethanol). Total phenolic content, total flavonoid content, antioxidant activities (ferric reducing/antioxidant power [FRAP], chelating activity, and DPPH scavenging activity) were also determined. Finally, we investigated its protective effect on DNA and lymphocytes damage response to oxidative stress. The results showed that the morphology of A. arguta pollen was similar to other pollen of the genus A. Lindl., but differs from them slightly in the specific morphology indicators. What is more, AAPE obtained by different extraction methods exhibited a protective effect against DNA oxidative damage, they also possessed a strong cytoprotection effect on mouse lymphocytes, especially the extraction obtained by method C, which had the highest total phenolic content (15.05 ± 0.34 mg GAE/g), strong ferrous ion-chelating ability (0.37 ± 0.023 mg Na EDTA/g), DPPH scavenging activity (IC  = 0.14 ± 0.04 mg/ml), and FRAP (7.13 ± 0.33 mg Trolox/g). This paper provided a new edible natural antioxidant for human which will protect us from oxidative stress. PRACTICAL APPLICATIONS: This study was the first to report the morphology of Actinidia arguta pollen by SEM analysis, and studied the effects of different extraction methods on antioxidant activities and cytoprotective effect of A. arguta pollen extract. We believed that our research makes a significant contribution to the literature, because the results of this research provide a reference for the development of a new edible natural antioxidant.
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http://dx.doi.org/10.1111/jfbc.13603DOI Listing
April 2021

A novel biocomposite scaffold with antibacterial potential and the ability to promote bone repair.

J Biomater Appl 2021 Sep 17;36(3):474-480. Epub 2021 Feb 17.

Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, Yuzhong, People's Republic of China.

Clinical treatment of bone defects caused by trauma, tumor resection and other bone diseases, especially bone defects that can lead to infection, remains a major challenge. Currently, autologous bone implantation is the gold standard for treatment of bone defects, but it is limited by secondary trauma and insufficient autologous material. Moreover, postoperative infection is an important factor affecting bone healing.AcN-RADARADARADARADA-CONH2 (RADA) is a new type of self-assembling peptide(SAP) composed of Arg,Ala,Asp and other amino acids was designed and prepared. The "RADA" self-assembling peptide hydrogels has excellent biological activity and it's completely biodegradable and non-toxic.It is also have been confirmed to promote cell proliferation, wound healing, tissue repair, and drug delivery. To promote bone regeneration and simultaneously prevent bacterial infection, we designed biocomposite scaffolds comprising RADA and calcium phosphate cement (CPC), termed RADA-CPC. The morphological features of the scaffold were characterized by scanning electron microscopy (SEM). In vitro studies demonstrated that RADA-CPC enhances osteoblast proliferation, differentiation and mineralization. In addition, the scaffold was used as a drug delivery system to treat postoperative infections by sustained release of ciprofloxacin (CIP). The RADA-CPC scaffold may have potential application prospects in orthopedics field because of its role in promoting bone repair and as a sustained-release drug carrier to prevent infections.
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http://dx.doi.org/10.1177/0885328221994448DOI Listing
September 2021

Efficacy and safety of the Amplatzer Duct Occluder II for ventricular septal defect closure: a meta-analysis.

Kardiol Pol 2021 04 19;79(4):401-409. Epub 2021 Jan 19.

Department of Ultrasonography, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Hospital of Traditional Chinese Medicine), Guangzhou, China.

Background: Transcatheter closure has become an effective and safe method to repair ventricular septal defects (VSDs). However, the devices used for VSD closure in the past frequently led to serious complications, such as complete atrioventricular block. The second‑generation device, the Amplatzer Duct Occluder (ADO II), was originally designed to block small‑ and medium‑sized patent ductus arteriosus. Interestingly, there are some reports of the use of the ADO II to close VSD because of less complications.

Aims: A meta‑analysis of the literature was performed to systematically investigate the efficacy and safety of the ADO II for VSD closure.

Methods: The Embase, PubMed, Cochrane, and Web of Science databases were searched for original studies on VSD closure with the ADO II up to March 15, 2020. The random‑effects model and summary rate were employed to estimate the success and complications of VSD closure with the ADO II.

Results: A total of 13 articles comprising 478 patients with VSD were included. The age of the patients ranged from 0.5 to 55.7 years. The overall estimated device‑implantation success rate was 99% (95% CI, 98%-100%). Residual shunts (pooled rate, 4%; 95% CI, 1%-7%) and postoperative aortic valve regurgitation (pooled rate, 0%; 95% CI, 0-1%) were common complications. Only 3 patients developed device embolism.

Conclusions: The ADO II may be a safer and more effective transcatheter closure device for patients with VSD due to its higher success rate and lower complication rate, as compared with other devices.
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http://dx.doi.org/10.33963/KP.15762DOI Listing
April 2021

Nonintrusive Fine-Grained Home Care Monitoring: Characterizing Quality of In-Home Postural Changes Using Bone-Based Human Sensing.

Sensors (Basel) 2020 Oct 18;20(20). Epub 2020 Oct 18.

Graduate School of System Informatics, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.

In contrast to the physical activities of able-bodied people at home, most people who require long-term specific care (e.g., bedridden patients and patients who have difficulty walking) usually show more low-intensity slow physical activities with postural changes. Although the existing devices can detect data such as heart rate and the number of steps, they have been increasing the physical burden relying on long-term wearing. The purpose of this paper is to realize a noninvasive fine-grained home care monitoring system that is sustainable for people requiring special care. In the proposed method, we present a novel technique that integrates inexpensive camera devices and bone-based human sensing technologies to characterize the quality of in-home postural changes. We realize a local process in feature data acquisition once per second, which extends from a computer browser to Raspberry Pi. Our key idea is to regard the changes of the bounding box output by standalone pose estimation models in the shape and distance as the quality of the pose conversion, body movement, and positional changes. Furthermore, we use multiple servers to realize distributed processing that uploads data to implement home monitoring as a web service. Based on the experimental results, we conveyed our findings and advice to the subject that include where the daily living habits and the irregularity of home care timings needed improvement.
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http://dx.doi.org/10.3390/s20205894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588905PMC
October 2020

WITHDRAWN: Application of Echocardiographic Information Technology in the Research of Infective Endocarditis and Its Neurological Complications.

Neurosci Lett 2020 Jun 27:135217. Epub 2020 Jun 27.

Department of Ultrasonography, Guangdong Provincial Hospital of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou City, 510120, Guangdong Province, China. Electronic address:

This article has been withdrawn at the request of the Editor-in-Chief. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal.
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http://dx.doi.org/10.1016/j.neulet.2020.135217DOI Listing
June 2020

Controlled release of basic fibroblast growth factor from a peptide biomaterial for bone regeneration.

R Soc Open Sci 2020 Apr 1;7(4):191830. Epub 2020 Apr 1.

Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Chongqing, Yuzhong District 400016, People's Republic of China.

Self-assembled peptide scaffolds based on D-RADA16 are an important matrix for controlled drug release and three-dimensional cell culture. In this work, D-RADA16 peptide hydrogels were coated on artificial bone composed of nano-hydroxyapatite/polyamide 66 (nHA/PA66) to obtain a porous drug-releasing structure for treating bone defects. The developed materials were characterized via transmission electron microscopy and scanning electron microscopy. The proliferation and adhesion of bone mesenchymal stem cells (BMSCs) were examined by confocal laser microscopy and CCK-8 experiments. The osteogenic ability of the porous materials towards bone BMSCs was examined by staining with Alizarin Red S and alkaline phosphatase, and bioactivity was evaluated . The results revealed that nHA/PA66/D-RADA16/bFGF reduces the degradation rate of D-RADA16 hydrogels and prolongs sustained release of bFGF, which would promote BMSCs proliferation, adhesion and osteogenesis and bone repair . Thus, it deserves more attention and is worthy of further research.
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http://dx.doi.org/10.1098/rsos.191830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211882PMC
April 2020

Toward Flexible and Efficient Home Context Sensing: Capability Evaluation and Verification of Image-Based Cognitive APIs.

Sensors (Basel) 2020 Mar 6;20(5). Epub 2020 Mar 6.

Graduate School of System Informatics, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.

Cognitive Application Program Interface (API) is an API of emerging artificial intelligence (AI)-based cloud services, which extracts various contextual information from non-numerical multimedia data including image and audio. Our interest is to apply image-based cognitive APIs to implement flexible and efficient context sensing services in a smart home. In the existing approach with machine learning by us, with the complexity of recognition object and the number of the defined contexts increases by users, it still requires directly manually labeling a moderate scale of data for training and continually try to calling multiple cognitive APIs for feature extraction. In this paper, we propose a novel method that uses a small scale of labeled data to evaluate the capability of cognitive APIs in advance, before training features of the APIs with machine learning, for the flexible and efficient home context sensing. In the proposed method, we exploit document similarity measures and the concepts (i.e., internal cohesion and external isolation) integrate into clustering results, to see how the capability of different cognitive APIs for recognizing each context. By selecting the cognitive APIs that relatively adapt to the defined contexts and data based on the evaluation results, we have achieved the flexible integration and efficient process of cognitive APIs for home context sensing.
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http://dx.doi.org/10.3390/s20051442DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085595PMC
March 2020

Postoperative bone marrow edema lasts no more than 6 months after uncomplicated arthroscopic double-row rotator cuff repair with PEEK anchors.

Knee Surg Sports Traumatol Arthrosc 2021 Jan 14;29(1):162-169. Epub 2020 Feb 14.

Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuanjiagang, Yuzhong District, Chongqing, 400016, China.

Purpose: To assess the natural evolution of the osseous reaction following arthroscopic double-row rotator cuff repair with PEEK anchors and to analyze its correlation with clinical shoulder function.

Methods: Between 2015 and 2017, 159 patients received arthroscopic double-row rotator cuff repair with PEEK anchors and underwent serial clinical and radiological follow-up (3, 6, 12, and 24 months). Radiological results were analyzed by tendon integrity, bone marrow edema, and peri-implant osteolysis. Clinical shoulder function was evaluated with the Constant score.

Results: One-hundred and seventeen patients were enrolled; among them, 63% demonstrated bone marrow edema around the anchors on postoperative 3-month MRI. The edema area percentage was 41% ± 7%. At 6 months, edema was only seen in 12% of cases, with an area percentage of 18% ± 5%. At 12 and 24 months, edema was rarely present. Fluid signals around the anchor were observed in 17.6%, 42.7%, 33.3%, and 21.0% of patients at 3, 6, 12, and 24 months, respectively; the tunnel widening values were 1.1 ± 0.4 mm, 1.8 ± 0.5 mm, 2.3 ± 0.6 mm, and 2.2 ± 0.7 mm at each follow-up, respectively. The sign of osteolysis was significantly more obvious around the lateral anchor than around the medial anchor. The presence of an osseous reaction was not correlated with worse clinical outcome.

Conclusion: Osseous reactions following arthroscopic rotator cuff repair are common and significant even with PEEK anchors. Bone marrow edema does not last more than 6 months in patients without complications. Peri-implant osteolysis is more evident around the lateral anchor than around the medial anchor and improves gradually over time. The sign of osteolysis is not correlated with clinical shoulder function. Based on these findings, surgeons should be cautious about bone marrow edema lasting more than 6 months following arthroscopic rotator cuff repair.

Level Of Evidence: Level IV.
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http://dx.doi.org/10.1007/s00167-020-05897-7DOI Listing
January 2021

Integrating Multiple Models Using Image-as-Documents Approach for Recognizing Fine-Grained Home Contexts.

Sensors (Basel) 2020 Jan 25;20(3). Epub 2020 Jan 25.

Graduate School of System Informatics, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.

To implement fine-grained context recognition that is accurate and affordable for general households, we present a novel technique that integrates multiple image-based cognitive APIs and light-weight machine learning. Our key idea is to regard every image as a document by exploiting "tags" derived by multiple APIs. The aim of this paper is to compare API-based models' performance and improve the recognition accuracy by preserving the affordability for general households. We present a novel method for further improving the recognition accuracy based on multiple cognitive APIs and four modules, fork integration, majority voting, score voting, and range voting.
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http://dx.doi.org/10.3390/s20030666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038333PMC
January 2020

Honey Polyphenols Ameliorate DSS-Induced Ulcerative Colitis via Modulating Gut Microbiota in Rats.

Mol Nutr Food Res 2019 12 12;63(23):e1900638. Epub 2019 Nov 12.

Collage of Food Science and Technology, Northwest University, 229 North TaiBai Road, Xi'an, 710069, China.

Scope: Ulcerative colitis (UC) is a multifaceted and recurrent immune disorder that requires long-term potent pharmacological treatment. Honey, as a natural food of nourishment and pharmaceutical value, has been found to defend against colitis.

Methods And Results: The effects of different constituents in honey are investigated on DSS-induced colitis in rats. Rats are given DSS, sugars, honey, polyphenols, or SASP for a week, with blood and colon samples collected for the biochemical parameters and inflammation-related gene analysis and colon contents for gut microbiota. The results show that pretreatments with honey polyphenols significantly improve SOD, GSH-Px, NO, and MPO levels and reduce DSS-induced colonic apoptosis, the colonic inflammatory cytokines IL-6, TNF-α, and TGF-β1 accompanied by downregulation of IL-1β, IL-6, TNF-α, and IFN-γ gene and upregulation of IκB-α gene. Furthermore, honey polyphenols and SASP show similar microbial community structure shifts and selective enrichment of key species. At the genus level, honey polyphenols significantly reduce the population of Bacteroides, Corynebacterium, and Proteus species. The correlation analysis indicates that colonic gene expression regulated by honey polyphenols is relative to the key species of gut microbiota.

Conclusions: Honey polyphenols improve intestinal inflammation and oxidative stress resistance via modulating gut microbiota, which is conducive to revealing the host-microbe interactions.
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http://dx.doi.org/10.1002/mnfr.201900638DOI Listing
December 2019

Jujube honey induces apoptosis in human hepatocellular carcinoma HepG2 cell via DNA damage, p53 expression, and caspase activation.

J Food Biochem 2019 11 1;43(11):e12998. Epub 2019 Aug 1.

College of Food Science and Technology, Northwest University, Xi'an, China.

Jujube honey, a very popular honey in China, has been shown to own numerous biological properties. However, the anticancer effect and the underlying mechanisms of jujube honey in HepG2 cells have not been investigated to date. This study was designed to evaluate jujube honey-induced apoptosis and its molecular mechanism in HepG2 and the following results were obtained. Firstly, treatment with jujube honey blocked cell cycle progression at the G0/G1 phase, reduced the mitochondrial membrane potential (Δψm), induced DNA damage, and upregulates p53 expression. Secondly, pro-apoptotic proteins Bax and Bad were upregulated and antiapoptotic proteins Bcl-2 and Bcl-xL were downregulated. Lastly, caspase activation and apoptotic death occurred in HepG2 cells. In conclusion, this study showed that jujube honey induces HepG2 cell apoptosis via DNA damage, p53 expression, and caspase activation. PRACTICAL APPLICATIONS: We demonstrate the molecular mechanism by which jujube honey triggers apoptosis in HepG2 cells. This new insight provided useful information on the use of jujube honey as a potential therapeutic and preventive agent.
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http://dx.doi.org/10.1111/jfbc.12998DOI Listing
November 2019

Rape bee pollen alleviates dextran sulfate sodium (DSS)-induced colitis by neutralizing IL-1β and regulating the gut microbiota in mice.

Food Res Int 2019 08 10;122:241-251. Epub 2019 Apr 10.

College of Food Science and Technology, Northwest University, 229 North TaiBai Road, Xi'an 710069, China; Bee Product Research Center of Shaanxi Province, Xi'an 710065, China. Electronic address:

Colitis is a major chronic disease in today's society. Consumption of phenolic compounds can protect against colitis. We examined the phenolic compounds of rape bee pollen extract (RPE) and investigated its prevention of DSS-induced colitis in C57BL/6 mice. HPLC-DAD-ECD analysis showed that 7 phenolic compounds were tentatively identified in RPE, of which kaempferol had the highest content (19.87 mg/g), followed by sinapic acid and rosmarinic acid. RPE at 21.2 g/kg BW (HD - high-dose group) and 10.6 g/kg BW (LD - low-dose group) ameliorated colon shortening, spleen swelling and colon weight reduction, improved the structure of colonic villi, glandular structures and crypts, which was further confirmed by conventional histological assessment, and inhibited the activities of related inflammatory cytokines. In particular, IL-1β expression was downregulated by approximately 90% compared with that of the model group. Additionally, treatment with RPE altered the gut microbial structure of mice with colitis, showing that the abundances of Allobaculum and Bacteroides were significantly reduced and the abundance of Lactobacillus was markedly increased. This study indicated that RPE could protect the colon from DSS-induced colitis by increasing antioxidant capacity, alleviating inflammation and regulating the gut microbiota. This paper was conducted to investigate the potent protective effects of RPE treatments on a mouse model of colitis and provided new ideas for the application of rape bee pollen.
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http://dx.doi.org/10.1016/j.foodres.2019.04.022DOI Listing
August 2019

Effects of honey-extracted polyphenols on serum antioxidant capacity and metabolic phenotype in rats.

Food Funct 2019 May;10(5):2347-2358

School of Chemical Engineering, Northwest University, 229 North TaiBai Road, Xi'an, 710069, China.

It is generally known that honey polyphenols have antioxidant capacity and numerous biological functions. However, their metabolic phenotype after digestion has not been studied. In this study, the effects of honey-extracted polyphenols (HEPs) on serum antioxidant capacity and metabolic phenotype were revealed for the first time. Herein, sixteen male Sprague-Dawley rats were randomly divided into two groups: one group was administered HEPs (200 mg per kilogram body weight dose) and the other group was fed distilled water three times. Then, 1 h after the last gavage, the metabolic profiling of serum was analyzed by UHPLC-Orbitrap-HRMS and multivariate statistical analysis, and the following results were obtained. At first, twenty-five metabolites, including polyphenols, unsaturated fatty acids, and amino acids, were selected as potential biomarkers. Then, metabolic pathway analysis showed that several amino acid metabolism pathways, ubiquinone and other terpenoid-quinone biosynthesis, arachidonic acid metabolism, nicotinate and nicotinamide metabolism, and inositol phosphate metabolism were affected. Association analysis demonstrated that the alteration of metabolites may be responsible for the increased serum antioxidant capacity. This new insight into the effects on the metabolic phenotype after HEP intake prompted the study on the biological functions and emerging health benefits of HEPs.
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http://dx.doi.org/10.1039/c8fo02138dDOI Listing
May 2019

Impact of SchisandraChinensis Bee Pollen on Nonalcoholic Fatty Liver Disease and Gut Microbiota in HighFat Diet Induced Obese Mice.

Nutrients 2019 Feb 6;11(2). Epub 2019 Feb 6.

College of Food Science and Technology, Northwest University, 229 North TaiBai Road, Xi'an 710069, China.

bee pollen has been used as a health food in China for centuries; however, its bioactive constituents and functions are not very clear. In this study, we investigated the phenolic compounds of bee pollen extract (SCPE) by UHPLC-Q-Orbitrap-HRMS/HPLC-DAD-ECD and its prevention from nonalcoholic fatty liver disease (NAFLD) and modulation of gut microbiota in high fat diet induced obese C57BL/6 mice. The results showed that 12 phenolic compounds were identified in SCPE, and naringenin, rutin and chrysin were the main constituents. The content of naringenin reached 1.89 mg/g, and total phenolic content (TPC) of SCPE were 101.83 mg GA/g. After obese mice were administrated with SCPE at 7.86 and 15.72 g/kg BW for 8 weeks, body weight gains were reduced by 18.23% and 19.37%. SCPE could decrease fasting blood glucose, cut down the lipid accumulation in serum and liver, lessen oxidative injury and inflammation in obesity mice. Moreover, SCPE could effectively inhibit the formation of NAFLD by inhibition of LXR-α, SREBP-1c and FAS genes expression, and modulate the structural alteration of gut microbiota in obesity mice. These findings suggested that SCPE could attenuate the features of the metabolism syndrome in obesity mice, which can be used to prevent obesity and NAFLD of human beings.
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http://dx.doi.org/10.3390/nu11020346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412546PMC
February 2019
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