Publications by authors named "Silvia Santos"

115 Publications

Simulation of pediatric intubation using a low-cost videolaryngoscope in the setting of the COVID-19 pandemic.

Arch Argent Pediatr 2021 08;119(4):270-272

Hospital Interzonal de Agudos "Eva Perón", San Martín, provincia de Buenos Aires, Argentina.

In patients with SARS-CoV-2 infection, endotracheal intubation is a procedure with a high risk for transmission. A videolaryngoscopy is a supplementary level of health care provider protection, but commercial videolaryngoscopes are expensive and not always available in pediatric intensive care units in Argentina. Our objective was to describe intubation practice using an infant head mannequin with a low-cost, handcrafted videolaryngoscope. Fifteen pediatricians with no prior experience using the device participated in an intubation practice in a head mannequin with a handcrafted videolaryngoscope. The average time for the first attempt was 116.4 seconds (95 % confidence interval [CI]: 84.8- 148.0) and, for the second one, 44.2 seconds (95 % CI: 27.7-60.6). Time decreased significantly for the second attempt (p: 0.0001). A successful intubation was achieved with the device in all attempts, and the procedure duration decreased with the second practice.
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http://dx.doi.org/10.5546/aap.2021.eng.270DOI Listing
August 2021

Gerontotechnology for fall prevention: nursing care for older adults with Parkinson.

Rev Esc Enferm USP 2021 23;55:e03748. Epub 2021 Jun 23.

Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Enfermagem, Florianópolis, SC, Brasil.

Objective: To assess the contribution of gerontotechnologies in nursing care to older adults with Parkinson's disease, aiming at the prevention of falls.

Method: Convergent Assistance Research, through the development and evaluation of gerontechnologies focused on the prevention of falls in older adults with Parkinson's Disease. Clinical evaluation, interviews and workshops with older adults were carried out, as well as analysis and judgment of the materials developed by ten judges certified in gerontology by the Brazilian Society of Geriatrics and Gerontology.

Results: Older adults need at least six years of study to understand the educational gerontotechnology development. The objectives related to the content, understanding of the text, illustration, presentation, motivation and cultural adaptation were achieved.

Conclusion: The educational gerontotechnology developed has relevant content, which can be used by older adults, family members and caregivers, to generate clarification of questions on the theme of fall prevention for older adults with Parkinson's Disease.
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http://dx.doi.org/10.1590/S1980-220X2020018403748DOI Listing
August 2021

Training and practice of nurses in Primary Care management: perspectives of Schön's Theory.

Rev Bras Enferm 2021 18;74(3):e20200461. Epub 2021 Jun 18.

Universidade Federal Fluminense. Niterói, Rio de Janeiro, Brazil.

Objectives: to analyze the process of permanent professional training of Primary Health Care nurse managers from the perspective of reflective practice theory, built by Donald Schön.

Methods: this is a theoretical-reflective study.

Results: the findings apply to nurse managers' practice to deal with the dilemmas that present themselves in the professional routine according to their knowledge, skills and attitudes, anchored in the reflection-in-action movement, which unites the process of permanent and practical training reflective, favoring the development of skills relevant to management.

Final Considerations: nursing health managers, during their ongoing training process, can develop skills to act effectively in Primary Care, reflecting in practice and on practice, deciding on the need to remain in training to address the issues arising from that same practice.
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http://dx.doi.org/10.1590/0034-7167-2020-0461DOI Listing
June 2021

Evaluation of the Spanish population coverage of a prospective HLA haplobank of induced pluripotent stem cells.

Stem Cell Res Ther 2021 04 13;12(1):233. Epub 2021 Apr 13.

Banc de Sang i Teixits, Edifici Dr. Frederic Duran i Jordà, Passeig Taulat 116, 08005, Barcelona, Spain.

Background: iPSC (induced pluripotent stem cells) banks of iPSC lines with homozygous HLA (human leukocyte antigen) haplotypes (haplobanks) are proposed as an affordable and off-the-shelf approach to allogeneic transplantation of iPSC derived cell therapies. Cord blood banks offer an extensive source of HLA-typed cells suitable for reprogramming to iPSC. Several initiatives worldwide have been undertaken to create national and international iPSC haplobanks that match a significant part of a population.

Methods: To create an iPSC haplobank that serves the Spanish population (IPS-PANIA), we have searched the Spanish Bone Marrow Donor Registry (REDMO) to identify the most frequently estimated haplotypes. From the top ten donors identified, we estimated the population coverage using the criteria of zero mismatches in HLA-A, HLA-B, and HLA-DRB1 with different stringencies: high resolution, low resolution, and beneficial mismatch.

Results: We have calculated that ten cord blood units from homozygous donors stored at the Spanish cord blood banks can provide HLA-A, HLA-B, and HLA-DRB1 matching for 28.23% of the population.

Conclusion: We confirm the feasibility of using banked cord blood units to create an iPSC haplobank that will cover a significant percentage of the Spanish and international population for future advanced therapy replacement strategies.
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http://dx.doi.org/10.1186/s13287-021-02301-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042859PMC
April 2021

Genomic insights of international clones of Haemophilus influenzae causing invasive infections in vaccinated and unvaccinated infants.

Microb Pathog 2021 Jan 28;150:104644. Epub 2020 Nov 28.

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, Brazil; Department of Clinical Analysis, Faculty of Pharmacy, University of São Paulo, São Paulo, Brazil. Electronic address:

The emergence of invasive Haemophilus influenzae infections in vaccinated patient is a public health concern. We have investigated the genomic basis of invasiveness and possible vaccine failure in H. influenzae causing invasive disease in vaccinated and unvaccinated children in Brazil. Three H. influenzae strains isolated from blood cultures of pediatric patients were sequenced. Serotype, MLST, resistome and virulome were predicted using bioinformatic tools, whereas single nucleotide polymorphisms (SNPs) analysis of cap loci and the presence of the putative virulence-enhancing IS1016-bexA partial deletion were predicted in silico. Infections were caused by H. influenzae type a (Hia), type b (Hib) and nontypeable (NTHi), belonging to international high-risk clones of sequence types ST23, ST6 and ST368, respectively, which have been identified in North American, European and Asian countries. Convergence of ampicillin resistance and virulence in Hib-ST6 was supported by bla and deletion in the bexA gene, whereas presence of SNPs in the cap-b locus was associated with antigenic modifications of the capsule structure. Hia-ST23 and NTHi-ST368 strains carried galU, lpsA, opsX, rfaF, iga1, lgtC and lic1/lic2 virulence genes, associated with colonization, adaptation and damage to the lung, or invasiveness. In summary, deletion in the bexA gene and presence of SNPs in the cap locus of Hib could be contributing to invasive disease and possible vaccine failure in pediatric patients, whereas serotype replacement of Hib with type "a" and NTHi strains denotes the ability of non-vaccine serotypes to re-colonize vaccinated patients. Finally, the dissemination of international high-risk clones of H. influenzae emphasizes the importance of monitoring changes in the molecular epidemiology of invasive H. influenzae disease.
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http://dx.doi.org/10.1016/j.micpath.2020.104644DOI Listing
January 2021

Economic assessment of a 40,000 t/y mixed plastic waste pyrolysis plant using direct heat treatment with molten metal: A case study of a plant located in Belgium.

Waste Manag 2021 Feb 13;120:698-707. Epub 2020 Nov 13.

Process and Chemical Engineering, School of Engineering, University College Cork, T12 YN60, Ireland. Electronic address:

Pyrolysis has been identified as an ideal process to recycle mixed plastic waste (MPW). This study investigates the economics of a 40,000 t/y MPW pyrolysis process, called PlastPyro, located in Belgium, to an accuracy of ±15% i.e. "Definite Estimate". The process uses molten metal in a direct heat treatment process to pyrolyse the waste. An internal rate of return (IRR) of 20% strongly indicates that a 40,000 t/y PlastPyro plant is financially attractive for private investors. The capital expenditure (CAPEX) is estimated to be €20.1 m or €26.1 m if the cost of capital is included. The operating expenditures (OPEX) of the plant are estimated €3.4 m per year. The sensitivity analysis shows six main variables having major impacts on the financial returns of a PlastPyro plant: (1) the addressable volume and quality of plastic waste, (2) the feedstock costs, (3) the capital and operating expenditures, (4) the revenues from the sale of the produced pyrolysis oil (P-oil), (5) the tipping fees and (6) the potential to co-locate a PlastPyro plant with a waste plastic sorting facility. For example, the 15-year low P-oil revenue price of €210/t results in an IRR of 20%; but on the 6th of March 2020 the P-oil price may have achieved €227/t, resulting in an IRR of 37%. The paper also shows that a reliable supply of MPW is available, and that reliable, accessible markets for the P-oil are available. Finally, cost estimates should state their accuracy and usually factorial cost estimates are not accurate enough to state the IRR.
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http://dx.doi.org/10.1016/j.wasman.2020.10.039DOI Listing
February 2021

Analysis of impact on tissue activity during COVID-19 outbreak: a survey of 8 banks in Spain.

Cell Tissue Bank 2020 Dec 15;21(4):557-562. Epub 2020 Oct 15.

Center for Blood Transfusion and Tissue Bank of Valencian Community, Valencia, Spain.

On March 19 World Health Organization declare the pandemic situation by outbreak coronavirus disease 2019 in the world. The pressure on the health care system has been very high in several countries. Spanish National Transplant Organization (ONT) have made many efforts in maintaining transplantation activity. Although the impact of the pandemic on organ activity has been analysed, to date, less data exist regarding the impact on tissue activity. The aim of this study has been the evaluation of the possible impact on the procurement, processing and distribution of tissues during the peak period of the pandemic COVID-19 in Spain. For this study, a multicentre analysis has been made with a survey of the tissue banks in Spain, during the period March 1 to April 30, 2020. Our data suggest that the impact of coronavirus in Spain has affected dramatically tissue donation but with a moderate effect on stored tissues such as bone, valves, vessels or skin. Tissue banks should prepare if future next pandemic waves surges so that tissue provision is guaranteed both in urgent and elective surgeries.
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http://dx.doi.org/10.1007/s10561-020-09853-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561239PMC
December 2020

Does vancomycin administered at an empirical dose ensure coverage of pediatric patients against gram-positive pathogens?

Rev Bras Ter Intensiva 2020 Jul-Sep;32(3):391-397

Centro de Farmacocinética-Clínica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo - São Paulo (SP), Brasil.

Objective: To investigate the vancomycin effectiveness against gram-positive pathogens with the minimum inhibitory concentration of 1mg/L in pediatric patients based on the area under the curve and the minimum inhibitory concentration ratio > 400.

Methods: A population of 22 pediatric patients (13 boys) admitted to the pediatric intensive care unit with preserved renal function was stratified in two groups (G1 < 7 years and G2 ≥ 7 years). After the fourth dose administered of vancomycin (10 - 15mg/kg every 6 hours) was administered, two blood samples were collected (third and fifth hours), followed by serum measurement by immunoassays to investigate the pharmacokinetics and antimicrobial coverage.

Results: There was no difference between the groups regarding dose, trough level or area under the curve. Coverage against gram-positive pathogens with a minimum inhibitory concentration of 1mg/L occurred in only 46% of patients in both groups. The pharmacokinetics in both groups were altered relative to the reference values, and the groups differed in regard to increased total body clearance and shortening of the biological half-life, which were more pronounced in younger patients.

Conclusion: A minimum empirical dose of 60mg/kg per day should be prescribed for pediatric patients in intensive care units with preserved renal function. The use of the ratio between the area under the curve and minimum inhibitory concentration in the evaluation of vancomycin coverage is recommended to achieve the desired outcome, since the pharmacokinetics are altered in these patients, which may impact the effectiveness of the antimicrobial.
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http://dx.doi.org/10.5935/0103-507X.20200067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595722PMC
August 2021

An Optimized Protocol for ChIP-Seq from Human Embryonic Stem Cell Cultures.

STAR Protoc 2020 Sep 18;1(2):100062. Epub 2020 Sep 18.

Quantitative Cell Biology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

Chromatin immunoprecipitation (ChIP) followed by next-generation sequencing is a powerful technique that characterizes the genome-wide DNA-binding profile of a protein of interest. The general ChIP-seq workflow has been applied widely to many sample types and target proteins, but sample-specific optimization of various steps is necessary to achieve high-quality data. This protocol is specifically optimized for cultured human embryonic stem cells (hESCs), including steps to check sample quality and non-specific enrichment of "hyper-ChIPable" regions prior to sequencing. For complete details on the use and execution of this protocol, please refer to Gunne-Braden et al. (2020).
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http://dx.doi.org/10.1016/j.xpro.2020.100062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501726PMC
September 2020

Institutionalized elderly people with dementia: an integrative review on nursing care.

Rev Bras Enferm 2020 29;73 Suppl 3:e20180419. Epub 2020 Jul 29.

Universidade Federal do Paraná, Curitiba, Paraná, Brazil.

Objective: To ascertain the characteristics of the nursing care provided to institutionalized elderly people with dementia.

Methods: Integrative review of studies published between 2013 and 2017, in English, Spanish, and Portuguese, from the databases BDENF, LILACS, CINAHL, PubMed, SciELO, TRIP, and National Guideline Clearing House. Thematic data analysis was used.

Results: From the data collected in the 41 included studies, three themes emerged: Care with a focus on the needs of elderly people in LTCIEs; Care based on the work process of the nursing team; and Shared care. Final considerations: Elderly care can take place from different perspectives: that of the elderly; the nursing team; and it can be shared among the different parties involved. The importance of communication must be stressed, as do the development of skills and attitudes of the team, as well as proper training and support, good environment in the care process, and an approach centered on the institutionalized elderly.
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http://dx.doi.org/10.1590/0034-7167-2018-0419DOI Listing
June 2021

The Apparent Requirement for Protein Synthesis during G2 Phase Is due to Checkpoint Activation.

Cell Rep 2020 07;32(2):107901

Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305-5174, USA; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307, USA. Electronic address:

Protein synthesis inhibitors (e.g., cycloheximide) block mitotic entry, suggesting that cell cycle progression requires protein synthesis until right before mitosis. However, cycloheximide is also known to activate p38 mitogen-activated protein kinase (MAPK), which can delay mitotic entry through a G2/M checkpoint. Here, we ask whether checkpoint activation or a requirement for protein synthesis is responsible for the cycloheximide effect. We find that p38 inhibitors prevent cycloheximide-treated cells from arresting in G2 phase and that G2 duration is normal in approximately half of these cells. The Wee1 inhibitor MK-1775 and Wee1/Myt1 inhibitor PD0166285 also prevent cycloheximide from blocking mitotic entry, raising the possibility that Wee1 and/or Myt1 mediate the cycloheximide-induced G2 arrest. Thus, protein synthesis during G2 phase is not required for mitotic entry, at least when the p38 checkpoint pathway is abrogated. However, M phase progression is delayed in cycloheximide-plus-kinase-inhibitor-treated cells, emphasizing the different requirements of protein synthesis for timely entry and completion of mitosis.
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http://dx.doi.org/10.1016/j.celrep.2020.107901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802425PMC
July 2020

From clocks to dominoes: lessons on cell cycle remodelling from embryonic stem cells.

FEBS Lett 2020 Jun 14. Epub 2020 Jun 14.

Quantitative Cell Biology Lab, The Francis Crick Institute, London, UK.

Cell division is a fundamental cellular process and the evolutionarily conserved networks that control cell division cycles adapt during development, tissue regeneration, cell de-differentiation and reprogramming, and a variety of pathological conditions. Embryonic development is a prime example of such versatility: fast, clock-like divisions hallmarking embryonic cells at early developmental stages become slower and controlled during cellular differentiation and lineage specification. In this review, we compare and contrast the unique cell cycle of mouse and human embryonic stem cells with that of early embryonic cells and of differentiated cells. We propose that embryonic stem cells provide an extraordinarily useful model system to understand cell cycle remodelling during embryonic-to-somatic transitions. We discuss how cell cycle networks help sustain embryonic stem cell pluripotency and self-renewal and how they safeguard cell identity and proper cell number in differentiated cells. Finally, we highlight the incredible diversity in cell cycle regulation within mammals and discuss the implications of studying cell cycle remodelling for understanding healthy and disease states.
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http://dx.doi.org/10.1002/1873-3468.13862DOI Listing
June 2020

Integrated approach in the control and management of skin neglected tropical diseases in three health districts of Côte d'Ivoire.

BMC Public Health 2020 Apr 17;20(1):517. Epub 2020 Apr 17.

Programme National de Lutte contre l'Ulcère de Buruli, Abidjan, Côte d'Ivoire.

Background: Neglected tropical diseases (NTDs) comprise 20 communicable diseases that are prevalent in rural poor and remote communities with less access to the health system. For effective and efficient control, the WHO recommends that affected countries implement integrated control interventions that take into account the different co-endemic NTDs in the same community. However, implementing these integrated interventions involving several diseases with different etiologies, requiring different control approaches and driven by different vertical programs, remains a challenge. We report here the results and lessons learned from a pilot test of this integrated approach based on integrated screening of skin diseases in three co-endemic health districts of Côte d'Ivoire, a West African country endemic for Buruli ulcer, leprosy and yaw.

Method: This cross-sectional study took place from April 2016 to March 2017 in 3 districts of Côte d'Ivoire co-endemic for BU, leprosy and yaws. The study was carried out in 6 stages: identification of potentially co-endemic communities; stakeholder training; social mobilization; mobile medical consultations; case detection and management; and a review meeting.

Results: We included in the study all patients with skin signs and symptoms at the screening stage who voluntarily accepted screening. In total, 2310 persons screened had skin lesions at the screening stage. Among them, 07 cases were diagnosed with Buruli ulcer. There were 30 leprosy cases and 15 yaws detected. Other types of ulcerations and skin conditions have been identified and represent the majority of cases detected. We learned from this pilot experience that integration can be successfully implemented in co-endemic communities in Côte d'Ivoire. Health workers are motivated and available to implement integrated interventions instead of interventions focused on a single disease. However, it is essential to provide capacity building, a minimum of drugs and consumables for the care of the patients identified, as well as follow-up of identified patients, including those with other skin conditions.

Conclusions: The results of this study show that the integration of activities can be successfully implemented in co-endemic communities under the condition of staff capacity building and minimal care of identified patients.
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http://dx.doi.org/10.1186/s12889-020-08632-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164353PMC
April 2020

GATA3 Mediates a Fast, Irreversible Commitment to BMP4-Driven Differentiation in Human Embryonic Stem Cells.

Cell Stem Cell 2020 05 16;26(5):693-706.e9. Epub 2020 Apr 16.

The Francis Crick Institute, London, UK. Electronic address:

During early development, extrinsic triggers prompt pluripotent cells to begin the process of differentiation. When and how human embryonic stem cells (hESCs) irreversibly commit to differentiation is a fundamental yet unanswered question. By combining single-cell imaging, genomic approaches, and mathematical modeling, we find that hESCs commit to exiting pluripotency unexpectedly early. We show that bone morphogenetic protein 4 (BMP4), an important differentiation trigger, induces a subset of early genes to mirror the sustained, bistable dynamics of upstream signaling. Induction of one of these genes, GATA3, drives differentiation in the absence of BMP4. Conversely, GATA3 knockout delays differentiation and prevents fast commitment to differentiation. We show that positive feedback at the level of the GATA3-BMP4 axis induces fast, irreversible commitment to differentiation. We propose that early commitment may be a feature of BMP-driven fate choices and that interlinked feedback is the molecular basis for an irreversible transition from pluripotency to differentiation.
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http://dx.doi.org/10.1016/j.stem.2020.03.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487786PMC
May 2020

Epidemiological study of pediatric severe sepsis in Argentina.

Arch Argent Pediatr 2019 06;117(3):S135-S156

Comité Nacional de Emergencias y Cuidados Críticos, Sociedad Argentina de Pediatría.

Introduction: Severe sepsis (SS) is one of the leading causes of pediatric mortality in Argentina. The objective was to describe the epidemiological characteristics and to analyze risk factors associated with mortality from SS among patients hospitalized in pediatric intensive care units (PICU) in Argentina.

Population And Methods: Observational, prospective cohort study among patients with SS hospitalized in PICUs in Argentina from 8/1/2008 to 9/30/2008.

Results: Two thousand five hundred and fifty nine patients were admitted at 59 PICUs. A total of 315 patients (335 events) were included. Patients' median age was 8 months (1-182). The frequency was 13.8 %, and 28-day mortality was 31.6 %. Patients with a higher mortality were those who were coming from rural areas, had comorbidities at the time of admission or a history of cancer, had shock or acute respiratory distress syndrome (ARDS), were administered low-dose corticosteroids or vasoactive drugs or more than 60 ml/kg of fluid in the first hour and in the first 6hours, and had severe malnutrition. Following adjustment by severity using a multivariate logistic regression model, only coming from a rural area, having shock or ARDS remained associated with 28-day mortality. Forty per cent of patients received antibiotics 60 minutes after diagnosis.

Conclusions: SS is an event with a high frequency and mortality in Argentina. In the severity adjusted multivariate analysis, only coming from a rural area, having ARDS and/or shock were associated with higher mortality.
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http://dx.doi.org/10.5546/aap.2019.eng.S135DOI Listing
June 2019

Effect of caffeine supplementation on exercise performance, power, markers of muscle damage, and perceived exertion in trained CrossFit men: a randomized, double-blind, placebo-controlled crossover trial.

J Sports Med Phys Fitness 2020 Feb 25;60(2):181-188. Epub 2019 Oct 25.

Clinical and Sports Nutrition Research Laboratory (LABINCE), Faculty of Nutrition, Federal University of Goias, Goiania, Brazil -

Background: Caffeine is a popular nutritional supplement among athletes. It is frequently used as an ergogenic aid to improve physical performance, delay fatigue, and increase muscle power. However, these effects have not been tested in CrossFit athletes. The aim of this study was to evaluate the effects of acute caffeine supplementation on workout performance, power, markers of muscle damage, and soreness in trained CrossFit men.

Methods: Nine men (28±2 years) with experience in CrossFit (2±0.3 years) were investigated in a randomized, double-blind, placebo-controlled crossover trial, with a 7-day washout between treatment periods. The athletes received anhydrous caffeine (CAF: 6 mg/kg body mass) or placebo (PLA) 60 minutes before a CrossFit workout with tasks that involved muscle strength, power, gymnastic movements, and metabolic conditioning. Blood samples were collected for creatine kinase (CK), C-reactive protein, and glucose determination. Workout performance, rating of perceived exertion (RPE), delayed-onset muscle soreness (DOMS), muscle strength (handgrip strength) and power (bench throw, jump squat and countermovement jump) were also evaluated.

Results: CAF resulted in higher glucose concentration after workout compared to PLA (+3.2 mmol/L, 95% CI: 2.1 to 4.3 vs. +1.5, 95% CI: -0.1 to 3.0 mmol/L, P=0.01). No differences were found between treatments in workout performance, CK, DOMS, RPE, muscle power and strength.

Conclusions: Acute CAF supplementation did not alter performance, markers of muscle damage, power, and RPE in trained CrossFit men.
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http://dx.doi.org/10.23736/S0022-4707.19.10043-6DOI Listing
February 2020

Tannin-Adsorbents for Water Decontamination and for the Recovery of Critical Metals: Current State and Future Perspectives.

Biotechnol J 2019 Dec 23;14(12):e1900060. Epub 2019 Sep 23.

Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials (LSRE-LCM), Chemical Engineering Department, Faculdade de Engenharia da Universidade do Porto, Porto, Portugal.

Biosorption is known as an effective way to clean-up water from organic and inorganic contaminants and has also emerged as a promising technology to recover critical substances. Tannins are renewable materials, coming from multiple vegetable sources. A variety of biosorbents have been developed from tannins, including tannin resins, rigid foams, composites with mesoporous silica, cellulose, collagen, and magnetic adsorbents. These materials have shown an excellent ability to uptake heavy-metal cations (Cd(II), Cu(II), Pb(II), Ni(II), Cr(III)), owning to the chelating ability provided by the plentiful adjacent hydroxyl groups. In addition, tannin-adsorbents have shown exceptional ability to remove Cr(VI), and to uptake Au(III) and Pd(II) from strong acidic solutions, which has evident application in the recovery of precious metals from e-wastes leaching. The fact that tannin-adsorbents can reduce the oxidation state of these adsorbates to Cr(III) and to elemental species of Au and Pd is interesting. Adsorption of dyes, surfactants, pharmaceuticals and antimony is also feasible, but the removal of certain metalloid species, such as arsenic and phosphate, seems to be limited even after applying chemical modifications. This article presents a systematic review on the preparation of tannin-adsorbents and their application in water decontamination and in the recovery of critical metals.
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http://dx.doi.org/10.1002/biot.201900060DOI Listing
December 2019

Eternal sunshine of the spotless cycle.

Mol Syst Biol 2019 04 5;15(4):e8864. Epub 2019 Apr 5.

Quantitative Cell Biology Laboratory, The Francis Crick Institute, London, UK.

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http://dx.doi.org/10.15252/msb.20198864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449696PMC
April 2019

Influence of the geometry of nanostructured hydroxyapatite and alginate composites in the initial phase of bone repair1.

Acta Cir Bras 2019 Feb 28;34(2):e201900203. Epub 2019 Feb 28.

PhD, Associate Professor, Health Sciences Institute, UFBA, Salvador-BA, Brazil. Conception of the study, critical revision.

Purpose: To analyze, histomorphologically, the influence of the geometry of nanostructured hydroxyapatite and alginate (HAn/Alg) composites in the initial phase of the bone repair.

Methods: Fifteen rats were distributed to three groups: MiHA - bone defect filled with HAn/Alg microspheres; GrHA - bone defect filled with HAn/Alg granules; and DV - empty bone defect; evaluated after 15 days postoperatively. The experimental surgical model was the critical bone defect, ≅8.5 mm, in rat calvaria. After euthanasia the specimens were embedded in paraffin and stained with hematoxylin and eosin, picrosirius and Masson-Goldner's trichrome.

Results: The histomorphologic analysis showed, in the MiHA, deposition of osteoid matrix within some microspheres and circumjacent to the others, near the bone edges. In GrHA, the deposition of this matrix was scarce inside and adjacent to the granules. In these two groups, chronic granulomatous inflammation was noted, more evident in GrHA. In the DV, it was observed bone neoformation restricted to the bone edges and formation of connective tissue with reduced thickness in relation to the bone edges, throughout the defect.

Conclusion: The geometry of the biomaterials was determinant in the tissue response, since the microspheres showed more favorable to the bone regeneration in relation to the granules.
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http://dx.doi.org/10.1590/s0102-8650201900203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6585910PMC
February 2019

Multiplex-PCR for diagnosis of bacterial meningitis.

Braz J Microbiol 2019 Apr 22;50(2):435-443. Epub 2019 Feb 22.

Faculdade de Ciências Farmacêuticas, Department of Clinical Chemistry and Toxicology, Universidade de São Paulo (USP), São Paulo, Brazil.

Considering the great lethality and sequels caused by meningitis, rapid diagnosis and prompt treatment initiation have a great impact on patient outcome. Here, we developed a multiplex-PCR for simultaneous detection of the four most prevalent bacterial pathogens directly in CSF samples. The multiplex-PCR was designed to detect the following genes: fbsA (Streptococcus agalactiae), lytA (Streptococcus pneumoniae), crtA (Neisseria meningitidis), p6 (Haemophilus influenzae), and 16S rRNA (any bacterial agent). The multiplex-PCR showed a DNA detection limit of 1 pg/μL. Among 447 CSF samples tested, 40 were multiplex-PCR positive, in which 27 and 13 had positive and negative bacterial culture, respectively. Our multiplex-PCR is fast, reliable, and easily implementable into a laboratory routine for bacterial meningitis confirmation, especially for patients who previously started antimicrobial therapy. Our molecular approach can substantially improve clinical diagnosis and epidemiological measures of meningitis disease burden.
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http://dx.doi.org/10.1007/s42770-019-00055-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6863191PMC
April 2019

[Symbolic capital of the work of the female health visitors of the Public Health Service Foundation in the State of Alagoas, Brazil].

Hist Cienc Saude Manguinhos 2018 Jul-Sep;25(3):817-839

Enfermeira, Instituição de Longa Permanência para Idosos Lar São Francisco de Assis.

This study of a historical nature analyzes the work of health visitors of the Public Health Service Foundation in Alagoas between 1960 and 1990 and the symbolic capital attributed to them. Documental and oral sources were used, the latter produced in interviews conducted using thematic oral history, given by the visitors, the physician and nurse. The information was analyzed based on the concepts of habitus, field, symbolic capital and distinction of Pierre Bourdieu, and revealed that health visitors were relevant in the healthcare of the communities where they worked with distinctive approaches to inculcate health practices through home visits and authority to reconfigure the hygienic habitus of families. This activity gave them symbolic power and capital legitimized by the community.
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http://dx.doi.org/10.1590/S0104-59702018000400012DOI Listing
November 2019

Bioadsorptive removal of Pb(II) from aqueous solution by the biorefinery waste of Fucus spiralis.

Sci Total Environ 2019 Jan 18;648:1201-1209. Epub 2018 Aug 18.

Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials (LSRE-LCM), Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.

In the context of developing the circular economy that enables a more sustainable use of the available resources and minimum waste generation, marine macroalgae have attracted the attention of researchers and industry due to its potential as a renewable resource. The current work aims to contribute to the design of a complete biorefinery processing, using Fucus spiralis seaweed (brown division) as starting material, and to determine the potential of the derived waste as biosorbent of heavy metals in aqueous solution. The macroalgae waste was obtained after the sequential separation of polyphenols, fucoidan and alginate extracts from F. spiralis. The capacity of F. spiralis waste for Pb(II) removal was successfully tested through biosorption tests. The uptake of Pb(II) was found to be very fast (few hours to achieve equilibrium). Tests performed with an initial metal concentration of 20 mg/L established the best adsorbent dosage (0.50 g/L) and an optimum pH of 4.5. In these conditions, lead was almost completely removed from the aqueous solution. Maximum adsorption capacity predicted by Langmuir model was 132 ± 14 mg/g (pH 4.5 ± 0.5, 20 °C). Desorption studies were conducted with different possible eluents. The best results were obtained with EDTA 0.1 mol/L, generating a 95 ± 4% desorption. F. spiralis biomass can therefore be submitted to a complete biorefinery processing and design in the attempt to fulfil the "zero-waste" concept.
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http://dx.doi.org/10.1016/j.scitotenv.2018.08.210DOI Listing
January 2019

Characterization of the novel HLA-A*80:04 allele identified in a Spanish volunteer donor.

HLA 2018 10 4;92(4):245-246. Epub 2018 Sep 4.

Histocompatibilidad, Centro de Transfusión de Madrid, Madrid, Spain.

HLA-A*80:04 shows one amino acid replacement, A76 > E, when compared with the A*80:01 allele.
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http://dx.doi.org/10.1111/tan.13372DOI Listing
October 2018

Arsenate and arsenite adsorption onto iron-coated cork granulates.

Sci Total Environ 2018 Nov 20;642:1075-1089. Epub 2018 Jun 20.

Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials (LSRE-LCM), Departamento de Engenharia Química, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal. Electronic address:

There is a growing demand for low-cost, effective adsorbents for arsenic removal from water intended for human consumption in affected rural areas. This work presents a novel adsorbent based on the coating of cork granulates with iron (oxy)hydroxides for the removal of As(III) and As(V) from aqueous matrices. A 2 fractional factorial design was used to determine the optimal conditions for the iron coating procedure. The optimal adsorbent was produced by coating low-density cork granulates with iron (oxy)hydroxides precipitated from a 0.05 mol L FeCl solution at pH 7, 20 °C temperature and 20 g L S/L ratio, in a single coating cycle. Arsenic adsorption was found to be dependent on pH, with inverse trends for As(III) and As(V). The iron leaching from the adsorbent was also taken into account to select the optimum pH, which was pH 9 for As(III) and pH 3 for As(V). Adsorption kinetics were better described by the pseudo-second-order model for As(III) and the Elovich model for As(V). Equilibrium was reached in 16 h for As(III) at pH 9 and 48 h for As(V) at pH 3. The isotherm models indicated different adsorption behaviours for As(III) and As(V), with better fits by Langmuir and Freundlich models, respectively. The Langmuir maximum adsorption capacity of iron-coated cork adsorbent for As(III) at pH 9 was 4.9 ± 0.3 mg g. However, at low equilibrium concentrations, As(V) adsorption was higher than As(III) (e.g. 2.1 ± 0.2 mg g in equilibrium with 0.16 ± 0.03 mg L). Speciation studies and XPS analyses indicated that no substantial oxidation of As(III) to As(V) occurred during the adsorption process. The study shows that iron coating can enhance both arsenate and arsenite adsorption capacity of cork materials, leading to an innovative natural adsorbent with high resilience and stability, with possible application in arsenic remediation.
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http://dx.doi.org/10.1016/j.scitotenv.2018.06.170DOI Listing
November 2018

[Pediatric emergency medicine specialty: Welcome!]

Arch Argent Pediatr 2018 08;116(4):298-300

Comité de Emergencias y Cuidados Críticos, Sociedad Argentina de Pediatría.

Pediatric Emergency Medicine has developed around the world as a subspecialty of pediatrics. There is plenty of data reflecting a growing need for its services. The subspecialty was initiated in the United States and Argentina, together with other Latin American countries, followed the same path. Nevertheless, there is yet much to be done. It is necessary to strengthen its promotion and to stimulate research and teaching activities in order to continue improving the quality of care delivered to the pediatric population, their families and society as a whole.
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http://dx.doi.org/10.5546/aap.2018.298DOI Listing
August 2018

Repercussions of hospitalization due to fall of the elderly: health care and prevention.

Rev Bras Enferm 2018 ;71 Suppl 2:763-769

Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.

Objective: To know the repercussions of the fall reported by the elderly and their caregiver during hospitalization in a public hospital in Florianópolis city from October to December 2014.

Method: Exploratory research with a qualitative approach, conducted by depth interviews with 16 participants, the eight elderly were hospitalized for falls and eight elderly caregivers. Data analysis were performed through the Thematic Content Analysis.

Results: It was evidenced the thematic axis: Faller Elderly supported by four thematic categories: Changes caused by Falls, I am a faller, I take care of me and Prevention of the Fall. The repercussions of the fall were evidenced in the impairment of the health condition, self-care and functional capacity. We observed the naturalization of the phenomenon and the passivity with the harmful consequences of the event. Final Considerations: The fall is valued the more negative its repercussion, such as the need for hospitalization and surgery. Managing the vulnerability of the elderly, especially in primary care, evaluating their comorbidities and their internal and external environment, will minimize unfavorable consequences and the social and financial cost of hospitalizations.
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http://dx.doi.org/10.1590/0034-7167-2017-0069DOI Listing
October 2018

Impact of a program aimed at reducing catheter-related infections implemented in nine pediatric intensive care units in Argentina.

Arch Argent Pediatr 2018 Apr;116(2):93-97

Hospital de Pediatría "J. P. Garrahan", Ciudad Autónoma de Buenos Aires.

Introduction: Catheter-related infections (CRIs) cause a high level of morbidity and mortality with the increasing use of hospital resources.

Objective: To describe the outcomes of a program implemented to reduce the rate of CRIs in pediatric intensive care units in Argentina.

Population And Methods: Collaborative, multi center, clinical-epidemiological, quasiexperimental, before-and-after intervention study. Children who had a central venous catheter during hospitalization in 9 pediatric intensive care units in the Autonomous City of Buenos Aires, Greater Buenos Aires, and other provinces between June 2011 and April 2012 were included. A bundle of measures based on health care staff training on catheter insertion, hand hygiene, and checklists was put into practice and implemented measures were monitored. The number and annual rate of CRIs and the rate of central venous catheter use before and after the program implementation were compared (Stata 8.0).

Results: The total number of CRIs was 117 and 74 before and after the intervention, respectively. The rate of CRIs was 8.6/1000 days of central venous catheter use and 5.8/1000 days before and after the intervention, respectively; RR: 0.82 (95% confidence interval: 0.68-0.98), p= 0.015. The rate of central venous catheter use decreased from 54% to 49%, a non-significant difference.

Conclusions: The program achieved a significant reduction in CRI rates. Based on the program, CRI surveillance was implemented in all participating pediatric intensive care units. Training and continuous surveillance are necessary to maintain and improve the outcomes accomplished with the program.
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http://dx.doi.org/10.5546/aap.2018.eng.93DOI Listing
April 2018

Genome sequence analysis of a hypermucoviscous/hypervirulent and MDR CTX-M-15/K19/ST29 Klebsiella pneumoniae isolated from human infection.

Pathog Dis 2017 12;75(9)

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, CEP 05508-000, Brazil.

The emergence of hypervirulent Klebsiella pneumoniae (hvKP) with multidrug resistance (MDR) profile is a worrisome public health issue. We report the first draft genome sequence of a hypermucoviscous (positive string test) and MDR K. pneumoniae serotype K19, belonging to ST29, isolated from human infection. This strain harboured multiple antimicrobial resistance genes, including blaCTX-M-15, besides yersiniabactin and type 3 fimbriae virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that K. pneumoniae K19/ST29 killed 100% of the larvae at 24 h post-infection, in a similar way to the known hypermucoviscous hvKP K1/ST23 lineage.
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http://dx.doi.org/10.1093/femspd/ftx121DOI Listing
December 2017

Obesity-Related Dietary Patterns and Health Status of Diabetes among At-Risk Latino College Students.

J Hispanic High Educ 2017 Oct 9;16(4):291-313. Epub 2016 Jun 9.

Oklahoma State University.

This study examined within-group differences in obesity-related dietary behaviors and the health status of 156 Latino students at-risk for diabetes due to family history. Approximately 58% of students were overweight/obese, with female students reporting a greater risk for diabetes. Consumption of meats, fried potatoes, breads, and tortillas was associated with higher BMI and diabetes risk indices. Differences in dietary behaviors were found based on students' college profile and sociodemographic characteristics. Implications for university-related intervention health initiatives are discussed.
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http://dx.doi.org/10.1177/1538192716653504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890233PMC
October 2017

OP9 Feeder Cells Are Superior to M2-10B4 Cells for the Generation of Mature and Functional Natural Killer Cells from Umbilical Cord Hematopoietic Progenitors.

Front Immunol 2017 30;8:755. Epub 2017 Jun 30.

Cell Therapy and Stem Cells Group, Basque Center for Transfusion and Human Tissues, Galdakao, Spain.

Adoptive natural killer (NK) cell therapy relies on the acquisition of large numbers of mature and functional NK cells. An option for future immunotherapy treatments is to use large amounts of NK cells derived and differentiated from umbilical cord blood (UCB) CD34 hematopoietic stem cells (HSCs), mainly because UCB is one of the most accessible HSC sources. In our study, we compared the potential of two stromal cell lines, OP9 and M2-10B4, for generation of mature and functional CD56 NK cells from UCB CD34 HSC. We generated higher number of CD56 NK cells in the presence of the OP9 cell line than when they were generated in the presence of M2-10B4 cells. Furthermore, higher frequency of CD56 NK cells was achieved earlier when cultures were performed with the OP9 cells than with the M2-10B4 cells. Additionally, we studied in detail the maturation stages of CD56 NK cells during the differentiation process. Our data show that by using both stromal cell lines, CD34 HSC differentiated into the terminal stages 4-5 of maturation resembled the differentiation pattern of human NK cells. Higher frequencies of more mature NK cells were reached earlier by using OP9 cell line than M2-10B4 cells. Alternatively, we observed that our NK cells expressed similar levels of granzyme B and perforin, and there were no significant differences between cultures performed in the presence of OP9 cell line or M2-10B4 cell line. Likewise, degranulation and cytotoxic activity against K562 target cells were very similar in both culture conditions. The results presented here provide an optimal strategy to generate high numbers of mature and functional NK cells , and point toward the use of the OP9 stromal cell line to accelerate the culture procedure to obtain them. Furthermore, this method could establish the basis for the generation of mature NK cells ready for cancer immunotherapy.
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http://dx.doi.org/10.3389/fimmu.2017.00755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491543PMC
June 2017
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