Publications by authors named "Siddhartha Pati"

12 Publications

  • Page 1 of 1

Microbial Fabrication of Nanomaterial and Its Role in Disintegration of Exopolymeric Matrices of Biofilm.

Front Chem 2021 24;9:690590. Epub 2021 May 24.

Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Haringhata, India.

Bacterial biofilms are responsible for the development of various chronic wound-related and implant-mediated infections and confer protection to the pathogenic bacteria against antimicrobial drugs and host immune responses. Hence, biofilm-mediated chronic infections have created a tremendous burden upon healthcare systems worldwide. The development of biofilms upon the surface of medical implants has resulted in the failure of various implant-based surgeries and therapies. Although different conventional chemical and physical agents are used as antimicrobials, they fail to kill the sessile forms of bacterial pathogens due to the resistance exerted by the exopolysaccharide (EPS) matrices of the biofilm. One of the major techniques used in addressing such a problem is to directly check the biofilm formation by the use of novel antibiofilm materials, local drug delivery, and device-associated surface modifications, but the success of these techniques is still limited. The immense expansion in the field of nanoscience and nanotechnology has resulted in the development of novel nanomaterials as biocidal agents that can be either easily integrated within biomaterials to prevent the colonization of microbial cells or directly approach the pathogen overcoming the biofilm matrix. The antibiofilm efficacies of these nanomaterials are accomplished by the generation of oxidative stresses and through alterations of the genetic expressions. Microorganism-assisted synthesis of nanomaterials paved the path to success in such therapeutic approaches and is found to be more acceptable for its "greener" approach. Metallic nanoparticles functionalized with microbial enzymes, silver-platinum nanohybrids (AgPtNHs), bacterial nanowires, superparamagnetic iron oxide (FeO), and nanoparticles synthesized by both magnetotactic and non-magnetotactic bacteria showed are some of the examples of such agents used to attack the EPS.
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http://dx.doi.org/10.3389/fchem.2021.690590DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181132PMC
May 2021

Amylases: Biofilm Inducer or Biofilm Inhibitor?

Front Cell Infect Microbiol 2021 27;11:660048. Epub 2021 Apr 27.

Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Haringhata, India.

Biofilm is a syntrophic association of sessile groups of microbial cells that adhere to biotic and abiotic surfaces with the help of pili and extracellular polymeric substances (EPS). EPSs also prevent penetration of antimicrobials/antibiotics into the sessile groups of cells. Hence, methods and agents to avoid or remove biofilms are urgently needed. Enzymes play important roles in the removal of biofilm in natural environments and may be promising agents for this purpose. As the major component of the EPS is polysaccharide, amylase has inhibited EPS by preventing the adherence of the microbial cells, thus making amylase a suitable antimicrobial agent. On the other hand, salivary amylase binds to amylase-binding protein of plaque-forming and initiates the formation of biofilm. This review investigates the contradictory actions and microbe-associated genes of amylases, with emphasis on their structural and functional characteristics.
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http://dx.doi.org/10.3389/fcimb.2021.660048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112260PMC
April 2021

Bacterial Biopolymer: Its Role in Pathogenesis to Effective Biomaterials.

Polymers (Basel) 2021 Apr 12;13(8). Epub 2021 Apr 12.

Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Haringhata 741249, India.

Bacteria are considered as the major cell factories, which can effectively convert nitrogen and carbon sources to a wide variety of extracellular and intracellular biopolymers like polyamides, polysaccharides, polyphosphates, polyesters, proteinaceous compounds, and extracellular DNA. Bacterial biopolymers find applications in pathogenicity, and their diverse materialistic and chemical properties make them suitable to be used in medicinal industries. When these biopolymer compounds are obtained from pathogenic bacteria, they serve as important virulence factors, but when they are produced by non-pathogenic bacteria, they act as food components or biomaterials. There have been interdisciplinary studies going on to focus on the molecular mechanism of synthesis of bacterial biopolymers and identification of new targets for antimicrobial drugs, utilizing synthetic biology for designing and production of innovative biomaterials. This review sheds light on the mechanism of synthesis of bacterial biopolymers and its necessary modifications to be used as cell based micro-factories for the production of tailor-made biomaterials for high-end applications and their role in pathogenesis.
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http://dx.doi.org/10.3390/polym13081242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069653PMC
April 2021

Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer Cells.

Polymers (Basel) 2021 Apr 18;13(8). Epub 2021 Apr 18.

Centre of Excellence, Khallikote University, Berhampur, Ganjam, Odisha 761008, India.

Diosgenin encapsulated PCL-Pluronic nanoparticles (PCL-F68-D-NPs) were developed using the nanoprecipitation method to improve performance in brain cancer (glioblastoma) therapy. The nanoparticles were characterized by dynamic light scattering (DLS)/Zeta potential, Fourier-transform infrared (FTIR) spectra, X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), and Transmission electron microscopy (TEM). The encapsulation efficiency, loading efficiency, and yield were calculated. The in vitro release rate was determined, and the kinetic model of diosgenin release was plotted and ascertained. The cytotoxicity was checked by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)assay against U87-MG cells (glioblastoma cell lines). The obtained nanoparticles demonstrated good size distribution, stability, morphology, chemical, and mechanical properties. The nanoparticles also possessed high encapsulation efficiency, loading efficiency, and yield. The release rate of Diosgenin was shown in a sustained manner. The in vitro cytotoxicity of PCL-F68-D-NPs showed higher toxicity against U87-MG cells than free Diosgenin.
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http://dx.doi.org/10.3390/polym13081322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073865PMC
April 2021

Population data and genetic characteristics of 12 X-STR loci using the Investigator® Argus X-12 Quality Sensor kit for the Kedayan population of Borneo in Malaysia.

Int J Legal Med 2021 Jul 30;135(4):1433-1435. Epub 2021 Mar 30.

Forensic Science Programme, School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia.

DNA profiling of X-chromosomal short tandem repeats (X-STR) has exceptional value in criminal investigations, especially for complex kinship and incest cases. In this study, Investigator® Argus X-12 Quality Sensor (QS) kits were successfully used to characterize 12 X-STR loci in 199 unrelated healthy Kedayan individuals living in Sabah and Sarawak, Malaysia. The LG1 haplogroup (DXS8378 - DXS10135 - DXS10148) has the largest HD (0.9799) as compared with all other closely linked haplotype groups examined (LG2; DXS7132-DXS10074-DXS10079, LG3; DXS10103-DXS10101-HPRTB and LG4; DXS10134-DXS7423-DXS10146). Data from statistical analysis showed that high combined of PD, PD, MEC_, MEC_, MEC_, and MEC_ values (0.999999994405922, 0.99999999999999, 0.999990463834938, 0.999999975914808, 0.999999975985006, and 0.999996491927194, respectively) in the Kedayan. In a two-dimensional scaling (MDS) plot and dendrogram constructed using allele frequencies at the 12 X-STR loci, Kedayan appear to be most closely related to their other Austronesian populations including the Malays and Filipinos as compared with other reference population groups. Findings from the present study thus demonstrate high genetic variability across the 12 tested X-STR loci and can be used for population studies and forensic applications.
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http://dx.doi.org/10.1007/s00414-021-02577-0DOI Listing
July 2021

Microbiologically-Synthesized Nanoparticles and Their Role in Silencing the Biofilm Signaling Cascade.

Front Microbiol 2021 25;12:636588. Epub 2021 Feb 25.

Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Haringhata, India.

The emergence of bacterial resistance to antibiotics has led to the search for alternate antimicrobial treatment strategies. Engineered nanoparticles (NPs) for efficient penetration into a living system have become more common in the world of health and hygiene. The use of microbial enzymes/proteins as a potential reducing agent for synthesizing NPs has increased rapidly in comparison to physical and chemical methods. It is a fast, environmentally safe, and cost-effective approach. Among the biogenic sources, fungi and bacteria are preferred not only for their ability to produce a higher titer of reductase enzyme to convert the ionic forms into their nano forms, but also for their convenience in cultivating and regulating the size and morphology of the synthesized NPs, which can effectively reduce the cost for large-scale manufacturing. Effective penetration through exopolysaccharides of a biofilm matrix enables the NPs to inhibit the bacterial growth. Biofilm is the consortia of sessile groups of microbial cells that are able to adhere to biotic and abiotic surfaces with the help extracellular polymeric substances and glycocalyx. These biofilms cause various chronic diseases and lead to biofouling on medical devices and implants. The NPs penetrate the biofilm and affect the quorum-sensing gene cascades and thereby hamper the cell-to-cell communication mechanism, which inhibits biofilm synthesis. This review focuses on the microbial nano-techniques that were used to produce various metallic and non-metallic nanoparticles and their "signal jamming effects" to inhibit biofilm formation. Detailed analysis and discussion is given to their interactions with various types of signal molecules and the genes responsible for the development of biofilm.
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http://dx.doi.org/10.3389/fmicb.2021.636588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947885PMC
February 2021

Horseshoe crab genomes reveal the evolution of genes and microRNAs after three rounds of whole genome duplication.

Commun Biol 2021 01 19;4(1):83. Epub 2021 Jan 19.

School of Life Sciences, Simon F.S. Li Marine Science Laboratory, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China.

Whole genome duplication (WGD) has occurred in relatively few sexually reproducing invertebrates. Consequently, the WGD that occurred in the common ancestor of horseshoe crabs ~135 million years ago provides a rare opportunity to decipher the evolutionary consequences of a duplicated invertebrate genome. Here, we present a high-quality genome assembly for the mangrove horseshoe crab Carcinoscorpius rotundicauda (1.7 Gb, N50 = 90.2 Mb, with 89.8% sequences anchored to 16 pseudomolecules, 2n = 32), and a resequenced genome of the tri-spine horseshoe crab Tachypleus tridentatus (1.7 Gb, N50 = 109.7 Mb). Analyses of gene families, microRNAs, and synteny show that horseshoe crabs have undergone three rounds (3R) of WGD. Comparison of C. rotundicauda and T. tridentatus genomes from populations from several geographic locations further elucidates the diverse fates of both coding and noncoding genes. Together, the present study represents a cornerstone for improving our understanding of invertebrate WGD events on the evolutionary fates of genes and microRNAs, at both the individual and population level. We also provide improved genomic resources for horseshoe crabs, of applied value for breeding programs and conservation of this fascinating and unusual invertebrate lineage.
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http://dx.doi.org/10.1038/s42003-020-01637-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815833PMC
January 2021

A review on introduced spp. and emerging concerns.

Heliyon 2020 Nov 4;6(11):e05370. Epub 2020 Nov 4.

Institute of Tropical Biodiversity and Sustainable Development, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.

Peacock bass ( spp.) originates from the Neotropical environments of Brazil and Venezuela but, through trade and smuggling for aquarium keeping, sport fishing and aquaculture, it is now an emerging concern. Yet, less is known for spp. distribution and its ability to invade new environments. Aimed to communicate on spp. ecology, biology and introduction schemes from Scopus, Web of Science, Google Scholar and also National Centre for Biotechnology Information, this review also contains management strategies for invading fish species. While spp. can displace native fish populations, this concern is explained using ecological functions, physiological demands, direct and secondary invasion, disease tolerance and parasite spillover. Briefly, spp. has rapid embryogenesis (72 h) and matures in short periods (11-12 months), giving it an advantage to colonize new environments. With a large appetite, this true piscivore gains territorial control over water bodies by making it their feeding and nursery grounds. Perceived as an emerging concern after becoming introduced, seal-off or sport fishing were used to manage spp. but, this practice is not sustainable for the entire ecosystem. Hence, we recommend bottom-up management that involves community participation because they interact with the fish and have knowledge about their environment.
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http://dx.doi.org/10.1016/j.heliyon.2020.e05370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648196PMC
November 2020

Characterization of phytochemicals, minerals and medicinal activities of bael ( L.) pulp and differently dried edible leathers.

Heliyon 2020 Oct 30;6(10):e05382. Epub 2020 Oct 30.

Department of Food Technology and Biochemical Engineering, Faculty of Engineering and Technology, Jadavpur University, Jadavpur, Kolkata 700032, India.

(L.) is a seasonal fruit that contains significant amounts of bioactives like, phenolic acids (gallic acids, 2,3-dihydroxy benzoic acid, chlorogenic acid, -coumaric acid, vanillic acid), flavonoid (rutin), organic acids (oxalic acid, tartaric acid, malic acid, lactic acid, acetic acid, citric acid, propionic acid, succinic acid, fumaric acid), vitamin C, vitamin B group (thiamine, niacin, pyridoxine, pantothenic acid, biotin, cobalamins, riboflavin), tocopherols (αtocopherol, βtocopherol, γtocopherol, δtocopherol), carotenes (αcarotene, βcarotene, γcarotene, δcarotene) and also rich in essential minerals (potassium, calcium, phosphorus, sodium, iron, copper, manganese). This study provides a comprehensive composition analysis (determined using RP-HPLC and Energy Dispersive X-Ray Fluorescence (EDXRF) Spectroscopy). medicinal activities (antioxidant activity, anti-inflammatory activity, anti-diabetic activity) are quantified for different bael samples. The study also investigates the changes of these bioactive components with freeze, sun, hot air, and microwave drying. The study gives a proper vision to preserve the nutraceutically rich pulp by converting it into fruit leather.
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http://dx.doi.org/10.1016/j.heliyon.2020.e05382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610326PMC
October 2020

Structural Characterization and Antioxidant Potential of Chitosan by γ-Irradiation from the Carapace of Horseshoe Crab.

Polymers (Basel) 2020 Oct 15;12(10). Epub 2020 Oct 15.

School of Biotechnology, GIET University, Gunupur 765022, Odisha, India.

Natural product extraction is ingenuity that permits the mass manufacturing of specific products in a cost-effective manner. With the aim of obtaining an alternative chitosan supply, the carapace of dead horseshoe crabs seemed feasible. This sparked an investigation of the structural changes and antioxidant capacity of horseshoe crab chitosan (HCH) by γ-irradiation using Co source. Chitosan was extracted from the horseshoe crab (; Müller) carapace using heterogeneous chemical -deacetylation of chitin, followed by the irradiation of HCH using Co at a dose-dependent rate of 10 kGy/hour. The average molecular weight was determined by the viscosimetric method. Regarding the chemical properties, the crystal-like structures obtained from γ-irradiated chitosan powders were determined using Fourier transfer infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analyses. The change in chitosan structure was evident with dose-dependent rates between 10 and 20 kGy/hour. The antioxidant properties of horseshoe crab-derived chitosan were evaluated in vitro. The 20 kGy γ-irradiation applied to chitosan changed the structure and reduced the molecular weight, providing sufficient degradation for an increase in antioxidant activity. Our findings indicate that horseshoe crab chitosan can be employed for both scald-wound healing and long-term food preservation due to its buffer-like and radical ion scavenging ability.
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http://dx.doi.org/10.3390/polym12102361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602389PMC
October 2020

Characterization dataset for pre- and post-irradiated shrimp waste chitosan.

Data Brief 2020 Oct 25;32:106081. Epub 2020 Jul 25.

Research Division, Association for Biodiversity Conservation and Research (ABC), Devine Colony, 756001 Balasore, Odisha, India.

This dataset presents morphological features, elemental composition and functional groups of different pre- and post-gamma (γ)-irradiated chitosan (10kGy & 20kGy) prepared from shrimp waste. The γ-irradiated chitosan was characterized using Fourier transfer infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analyses. Thermogravimetry/differential thermal analysis (TG/DTA) were performed using Perkin Elmer Pyris Diamond DSC with a heating rate of 10 °C/minute and dynamic synthetic atmospheric air set at flow rate of 100 ml/minute. We observed γ-irradiated chitosan to have shorter polymer size, small pores and compacted structure with active alkyl and hydroxyl groups when compared to non-irradiated chitosan. Our data provides baseline understanding for structure of shrimp chitosan after Co exposure which means, the biopolymer becomes more stable and is considered suitable for vast food industry applications.
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http://dx.doi.org/10.1016/j.dib.2020.106081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397402PMC
October 2020

Dataset on leaching properties of coal ashes from Malaysian coal power plant.

Data Brief 2020 Aug 8;31:105843. Epub 2020 Jun 8.

Institute of Tropical Biodiversity and Sustainable Development, Universiti Malaysia Terengganu, 21300 Kuala Terengganu, Terengganu, Malaysia.

Coal combustion by-products (CCPs) (i.e. fly (FA) and bottom (BA) ashes) generated by power plants contain heavy metals. This research presents leaching properties of coal ashes (FA and BA) collected from Jimah coal-fired power station, Port Dickson, Negeri Sembilan using USEPA standard methods namely toxicity characteristic leaching procedure (TCLP), and synthetic precipitation leaching procedure (SPLP). Heavy metals like lead (Pb), zinc (Zn), copper (Cu) and arsenic (As) were quantified using atomic absorption spectrometer (AAS). The leached of heavy metals fluxes were Cu < Zn < Pb < As. As leached the most whilst indicating of possible contamination from As. Overall, the ranges of leached concentration were adhered to permissible limits of hazardous waste criteria for metal (Pb and As) and industrial effluent (Zn and Cu). The presented data has potential reuse as reference for the coal ash concrete mixed design application in construction industries.
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http://dx.doi.org/10.1016/j.dib.2020.105843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306575PMC
August 2020